AlfalfaGEDB | Msa1086470

M. sativa cultivar zhongmu-4 / Msa1086470

KEGG: K02111|K02132
GO: -
PFAM: PF00006.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1086470	YP_009368364.1	79.155	355	5	4	1	286	77	431	0.0	528

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1086470	sp\|Q33C53\|ATPA_NICTO	79.155	355	5	4	1	286	77	431	0.0	528

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1086470	A0A4P8F197	80.282	355	1	4	1	286	77	431	0.0	535

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001040	Msa1086470	0.810176	1.313080e-50	-8.615850e-47
Msa0002110	Msa1086470	0.807909	4.015316e-50	-8.615850e-47
Msa0002690	Msa1086470	0.845524	3.849894e-59	-8.615850e-47
Msa0002700	Msa1086470	0.845954	2.941325e-59	-8.615850e-47
Msa0004180	Msa1086470	0.816215	6.202709e-52	-8.615850e-47
Msa0016830	Msa1086470	0.811255	7.669908e-51	-8.615850e-47
Msa0021570	Msa1086470	0.844021	9.782951e-59	-8.615850e-47
Msa0027120	Msa1086470	0.800487	1.408225e-48	-8.615850e-47
Msa0028410	Msa1086470	0.832648	8.383975e-56	-8.615850e-47
Msa0028450	Msa1086470	0.824300	8.695454e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1086470	MtrunA17_Chr4g0015651	79.718	355	3	4	1	286	77	431	0.0	529
Msa1086470	MtrunA17_Chr4g0016741	79.718	355	3	4	1	286	77	431	0.0	529
Msa1086470	MtrunA17_CPg0492941	79.718	355	3	4	1	286	77	431	0.0	529
Msa1086470	MtrunA17_MTg0490471	56.044	364	82	5	1	286	78	441	4.82e-122	358
Msa1086470	MtrunA17_Chr2g0278821	84.656	189	15	2	112	286	24	212	1.69e-107	314
Msa1086470	MtrunA17_Chr2g0286451	65.574	183	8	2	3	130	7	189	4.21e-70	215
Msa1086470	MtrunA17_Chr4g0061471	73.333	75	8	1	24	86	1	75	1.82e-28	104
Msa1086470	MtrunA17_Chr4g0040631	95.745	47	2	0	1	47	77	123	3.09e-22	90.1
Msa1086470	MtrunA17_Chr1g0211291	28.283	297	140	7	11	234	84	380	4.17e-21	92.8
Msa1086470	MtrunA17_Chr7g0250051	28.041	296	141	7	11	234	83	378	1.42e-20	91.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1086470	ATCG00120.1	77.465	355	11	4	1	286	77	431	0.0	517
Msa1086470	ATMG01190.1	53.846	364	90	5	1	286	78	441	1.44e-116	345
Msa1086470	AT2G07698.1	54.121	364	89	5	1	286	348	711	1.51e-114	347
Msa1086470	AT4G38510.1	28.378	296	140	7	11	234	83	378	1.12e-21	94.7
Msa1086470	AT4G38510.3	28.378	296	140	7	11	234	83	378	1.12e-21	94.7
Msa1086470	AT4G38510.4	28.378	296	140	7	11	234	83	378	1.12e-21	94.7
Msa1086470	AT4G38510.2	28.378	296	140	7	11	234	83	378	1.12e-21	94.7
Msa1086470	AT4G38510.5	28.378	296	140	7	11	234	90	385	1.15e-21	94.7
Msa1086470	AT1G76030.1	28.378	296	140	7	11	234	82	377	9.54e-21	92.0
Msa1086470	AT1G20260.1	36.000	125	79	1	111	234	254	378	2.17e-15	76.3

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Find 69 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCCATGAAACAGGTAGC+TGG	0.558354	7_2:+87411582	Msa1086470:intron
AAATATTTGGCCATCTGTAA+TGG	0.434228	7_2:-87411472	None:intergenic
AAATTTCAGCTTCGGAATCT+CGG	0.492871	7_2:+87410824	Msa1086470:CDS
AAGCTGAAACATCTCCTGAT+TGG	0.409535	7_2:-87411428	None:intergenic
AATCAGACCAGCTATTAATG+TGG	0.625394	7_2:+87411521	Msa1086470:CDS
AATGTAATTTCCATTACAGA+TGG	0.557336	7_2:+87411462	Msa1086470:CDS
ACCAGTAAGTGAGGGTTATT+TGG	0.166311	7_2:+87410756	Msa1086470:CDS
ACCATCAATAGGTTTAGCTA+GGG	0.601516	7_2:-87410794	None:intergenic
ACGCAAAATCAATTGGCAAG+AGG	0.645378	7_2:+87411681	Msa1086470:CDS
AGATACAATTCTCAATCAAC+AGG	0.401486	7_2:+87411005	Msa1086470:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGATTCTATGATCCCTATA+GGG	+	chr7_2:87410923-87410942	Msa1086470:CDS	30.0%
AGATACAATTCTCAATCAAC+AGG	+	chr7_2:87411005-87411024	Msa1086470:CDS	30.0%
GATACAATTCTCAATCAACA+GGG	+	chr7_2:87411006-87411025	Msa1086470:CDS	30.0%
GTAACTACTTTACAAGAAAG+AGG	+	chr7_2:87411090-87411109	Msa1086470:intron	30.0%
AACTACTTTACAAGAAAGAG+GGG	+	chr7_2:87411092-87411111	Msa1086470:intron	30.0%
AATGGAGTACACTATTATAG+TGG	+	chr7_2:87411116-87411135	Msa1086470:intron	30.0%
TTCGTAATAGAAGAGACATT+TGG	-	chr7_2:87411281-87411300	None:intergenic	30.0%
GCTAAATTAAGTTCTCAGTT+AGG	+	chr7_2:87411369-87411388	Msa1086470:CDS	30.0%
AAATATTTGGCCATCTGTAA+TGG	-	chr7_2:87411475-87411494	None:intergenic	30.0%
GATCGGCAGATAAAAATATT+TGG	-	chr7_2:87411488-87411507	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	87410691	87412164	87410691	ID=Msa1086470;Name=Msa1086470
chr7_2	mRNA	87410691	87412164	87410691	ID=Msa1086470-mRNA-1;Parent=Msa1086470;Name=Msa1086470-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0\|0\|0\|0.83\|0\|0\|6\|0\|288
chr7_2	exon	87410691	87410845	87410691	ID=Msa1086470-mRNA-1:exon:23811;Parent=Msa1086470-mRNA-1
chr7_2	exon	87410882	87411060	87410882	ID=Msa1086470-mRNA-1:exon:23812;Parent=Msa1086470-mRNA-1
chr7_2	exon	87411190	87411390	87411190	ID=Msa1086470-mRNA-1:exon:23813;Parent=Msa1086470-mRNA-1
chr7_2	exon	87411397	87411560	87411397	ID=Msa1086470-mRNA-1:exon:23814;Parent=Msa1086470-mRNA-1
chr7_2	exon	87411597	87411752	87411597	ID=Msa1086470-mRNA-1:exon:23815;Parent=Msa1086470-mRNA-1
chr7_2	exon	87412153	87412164	87412153	ID=Msa1086470-mRNA-1:exon:23816;Parent=Msa1086470-mRNA-1
chr7_2	CDS	87410691	87410845	87410691	ID=Msa1086470-mRNA-1:cds;Parent=Msa1086470-mRNA-1
chr7_2	CDS	87410882	87411060	87410882	ID=Msa1086470-mRNA-1:cds;Parent=Msa1086470-mRNA-1
chr7_2	CDS	87411190	87411390	87411190	ID=Msa1086470-mRNA-1:cds;Parent=Msa1086470-mRNA-1
chr7_2	CDS	87411397	87411560	87411397	ID=Msa1086470-mRNA-1:cds;Parent=Msa1086470-mRNA-1
chr7_2	CDS	87411597	87411752	87411597	ID=Msa1086470-mRNA-1:cds;Parent=Msa1086470-mRNA-1
chr7_2	CDS	87412153	87412164	87412153	ID=Msa1086470-mRNA-1:cds;Parent=Msa1086470-mRNA-1

Gene Sequence

>Msa1086470

ATGGGTGATGGTTTGCTGATACAAGAGGGAAGTTCGGTAAAAGCAACAGGAAGAATTGCTCAGATACCAGTAAGTGAGGGTTATTTGGGTCGTGTTGTAAATGCCCTAGCTAAACCTATTGATGGTCGAGGAGAAATTTCAGCTTCGGAATCTCGACGTTCCGTGTATGAGCCTCTTCAAACGGGACTTATTGCTATTGATTCTATGATCCCTATAGGGCGTGGCCAGCGAGAATTAATTATTGGGGACAGACAAACAGGTAAAACAGCAGTAGCAACAGATACAATTCTCAATCAACAGGGACAAAATGTAATATGTGTTTATGTAGCTATTGGTGCAGCTCTGGCTGAATATTTTATGTACCGTGAACGTCACACTTTAATCATTTATGACGATCCCTCCAAACAAGCACAGGCTTATCGCCAAATGTCTCTTCTATTACGAAGACCACCAGGTCGCGAAGCGTATCCCGGAGATGTTTTTTATTTACATTCTCGTCTTTTGGAAAGAGCCGCTAAATTAAGTTCTCAGTTAGGAAGTATGACTGCTTTACCAATAGTTGAGACCCAATCAGGAGATGTTTCAGCTTATATTCCTACTAATGTAATTTCCATTACAGATGGCCAAATATTTTTATCTGCCGATCTATTCAATGCTGGAATCAGACCAGCTATTAATGTGGGTATTTCCGTTTCCAGAGTAGCTGGTAAATTAAAATTGGAATTGGCACAATTCGCAGAATTAGAAGCTTTTGCACAATTCGCTTCTGATCTCGATAAAGCTACGCAAAATCAATTGGCAAGAGGTCAACGATTGCGCGAGTTGCTTAAACAATCCCAATCAGCTCCTCTCACTATACGAATATAA

Protein sequence

>Msa1086470

MGDGLLIQEGSSVKATGRIAQIPVSEGYLGRVVNALAKPIDGRGEISASESRRSVYEPLQTGLIAIDSMIPIGRGQRELIIGDRQTGKTAVATDTILNQQGQNVICVYVAIGAALAEYFMYRERHTLIIYDDPSKQAQAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSSQLGSMTALPIVETQSGDVSAYIPTNVISITDGQIFLSADLFNAGIRPAINVGISVSRVAGKLKLELAQFAELEAFAQFASDLDKATQNQLARGQRLRELLKQSQSAPLTIRI*