AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0268400


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268400 MtrunA17_CPg0492961 95.489 133 6 0 1 133 50 182 7.80e-89 256
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268400 ATCG00130.1 81.538 130 24 0 1 130 52 181 1.53e-72 215

Find 25 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCTAATATTGATATGTTT+GGG 0.275522 2_2:-70214203 Msa0268400:CDS
GGTGCTAATATTGATATGTT+TGG 0.316362 2_2:-70214204 Msa0268400:CDS
CTACTTTCCTTAAGCGGGCC+CGG 0.340021 2_2:+70214469 None:intergenic
TAATTCAATTTATACAACTT+TGG 0.388293 2_2:-70214379 Msa0268400:CDS
TGAATTGAAAGATTCATGTT+TGG 0.391532 2_2:-70214644 None:intergenic
AGAAAGCCCGGGCCCGCTTA+AGG 0.414131 2_2:-70214476 Msa0268400:CDS
CGTTTCTACTTTCCTTAAGC+GGG 0.444690 2_2:+70214464 None:intergenic
GCGGATCGGTTTCGAGTGAA+TGG 0.446998 2_2:-70214438 Msa0268400:CDS
TTCAAAATTCAGAAGAACTA+CGG 0.449969 2_2:-70214521 Msa0268400:CDS
GGAAGCAGCCGTTGAACAGC+TGG 0.450450 2_2:-70214499 Msa0268400:CDS
ACAAGCATTACAAGGAGCTC+TGG 0.452163 2_2:-70214274 Msa0268400:CDS
TACTTTCCTTAAGCGGGCCC+GGG 0.461743 2_2:+70214470 None:intergenic
TTGAACAGCTGGAGAAAGCC+CGG 0.472109 2_2:-70214488 Msa0268400:CDS
GGCTTTCTCCAGCTGTTCAA+CGG 0.504402 2_2:+70214491 None:intergenic
TCAAAATTCAGAAGAACTAC+GGG 0.510719 2_2:-70214520 Msa0268400:CDS
CCGTTTCTACTTTCCTTAAG+CGG 0.521089 2_2:+70214463 None:intergenic
CAAGCATTACAAGGAGCTCT+GGG 0.568354 2_2:-70214273 Msa0268400:CDS
AAGTAGAAACGGAAGCGGAT+CGG 0.570689 2_2:-70214452 Msa0268400:CDS
CCGCTTAAGGAAAGTAGAAA+CGG 0.573518 2_2:-70214463 Msa0268400:CDS
TGAACAGCTGGAGAAAGCCC+GGG 0.575165 2_2:-70214487 Msa0268400:CDS
TGCTAATATTGATATGTTTG+GGG 0.586102 2_2:-70214202 Msa0268400:CDS
CGATTAATCAAGTGCAACAG+AGG 0.596922 2_2:-70214305 Msa0268400:CDS
GAGTTACATTTACGTACGAT+CGG 0.617220 2_2:-70214225 Msa0268400:CDS
AAGGAAAGTAGAAACGGAAG+CGG 0.622485 2_2:-70214457 Msa0268400:CDS
GATTAATCAAGTGCAACAGA+GGG 0.644246 2_2:-70214304 Msa0268400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTCAATTTATACAACTT+TGG - chr2_2:70214429-70214448 Msa0268400:CDS 15.0%
! TTCAAAATTCAGAAGAACTA+CGG - chr2_2:70214287-70214306 Msa0268400:CDS 25.0%
!! GATTCATTTTGAACAACAAA+GGG - chr2_2:70214480-70214499 Msa0268400:CDS 25.0%
! GTGCTAATATTGATATGTTT+GGG - chr2_2:70214605-70214624 Msa0268400:intron 25.0%
! TGCTAATATTGATATGTTTG+GGG - chr2_2:70214606-70214625 Msa0268400:intron 25.0%
TCAAAATTCAGAAGAACTAC+GGG - chr2_2:70214288-70214307 Msa0268400:CDS 30.0%
! CAATTTTTCTCGTTCTATCT+CGG + chr2_2:70214401-70214420 None:intergenic 30.0%
! CGATTCATTTTGAACAACAA+AGG - chr2_2:70214479-70214498 Msa0268400:CDS 30.0%
! GTTTTACAACAAGCATTACA+AGG - chr2_2:70214526-70214545 Msa0268400:CDS 30.0%
GGTGCTAATATTGATATGTT+TGG - chr2_2:70214604-70214623 Msa0268400:intron 30.0%
GATTAATCAAGTGCAACAGA+GGG - chr2_2:70214504-70214523 Msa0268400:CDS 35.0%
GAGTTACATTTACGTACGAT+CGG - chr2_2:70214583-70214602 Msa0268400:intron 35.0%
CGTTTCTACTTTCCTTAAGC+GGG + chr2_2:70214347-70214366 None:intergenic 40.0%
CCGTTTCTACTTTCCTTAAG+CGG + chr2_2:70214348-70214367 None:intergenic 40.0%
CCGCTTAAGGAAAGTAGAAA+CGG - chr2_2:70214345-70214364 Msa0268400:CDS 40.0%
AAGGAAAGTAGAAACGGAAG+CGG - chr2_2:70214351-70214370 Msa0268400:CDS 40.0%
CGATTAATCAAGTGCAACAG+AGG - chr2_2:70214503-70214522 Msa0268400:CDS 40.0%
AAGTAGAAACGGAAGCGGAT+CGG - chr2_2:70214356-70214375 Msa0268400:CDS 45.0%
ACAAGCATTACAAGGAGCTC+TGG - chr2_2:70214534-70214553 Msa0268400:CDS 45.0%
CAAGCATTACAAGGAGCTCT+GGG - chr2_2:70214535-70214554 Msa0268400:CDS 45.0%
GGCTTTCTCCAGCTGTTCAA+CGG + chr2_2:70214320-70214339 None:intergenic 50.0%
TTGAACAGCTGGAGAAAGCC+CGG - chr2_2:70214320-70214339 Msa0268400:CDS 50.0%
TGAACAGCTGGAGAAAGCCC+GGG - chr2_2:70214321-70214340 Msa0268400:CDS 55.0%
TACTTTCCTTAAGCGGGCCC+GGG + chr2_2:70214341-70214360 None:intergenic 55.0%
CTACTTTCCTTAAGCGGGCC+CGG + chr2_2:70214342-70214361 None:intergenic 55.0%
! GCGGATCGGTTTCGAGTGAA+TGG - chr2_2:70214370-70214389 Msa0268400:CDS 55.0%
GGAAGCAGCCGTTGAACAGC+TGG - chr2_2:70214309-70214328 Msa0268400:CDS 60.0%
AGAAAGCCCGGGCCCGCTTA+AGG - chr2_2:70214332-70214351 Msa0268400:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 70214179 70214651 70214179 ID=Msa0268400;Name=Msa0268400
chr2_2 mRNA 70214179 70214651 70214179 ID=Msa0268400-mRNA-1;Parent=Msa0268400;Name=Msa0268400-mRNA-1;_AED=0.47;_eAED=0.48;_QI=0|0|0|1|1|1|2|0|133
chr2_2 exon 70214645 70214651 70214645 ID=Msa0268400-mRNA-1:exon:22528;Parent=Msa0268400-mRNA-1
chr2_2 exon 70214179 70214573 70214179 ID=Msa0268400-mRNA-1:exon:22527;Parent=Msa0268400-mRNA-1
chr2_2 CDS 70214645 70214651 70214645 ID=Msa0268400-mRNA-1:cds;Parent=Msa0268400-mRNA-1
chr2_2 CDS 70214179 70214573 70214179 ID=Msa0268400-mRNA-1:cds;Parent=Msa0268400-mRNA-1
Gene Sequence

>Msa0268400

ATGTTTGATAATCGAAAACAGAGAATCTTGAGAACTATTCAAAATTCAGAAGAACTACGGGAAGCAGCCGTTGAACAGCTGGAGAAAGCCCGGGCCCGCTTAAGGAAAGTAGAAACGGAAGCGGATCGGTTTCGAGTGAATGGTTATTCCGAGATAGAACGAGAAAAATTGAATTTAATTAATTCAATTTATACAACTTTGGAACAATTCGAAAATTACAAAAATGAAACGATTCATTTTGAACAACAAAGGGCGATTAATCAAGTGCAACAGAGGGTTTTACAACAAGCATTACAAGGAGCTCTGGGAACTCTGAATAGTTGCTTAAACAACGAGTTACATTTACGTACGATCGGTGCTAATATTGATATGTTTGGGGCGATGAAAGAAAAAAAGAACTGA

Protein sequence

>Msa0268400

MFDNRKQRILRTIQNSEELREAAVEQLEKARARLRKVETEADRFRVNGYSEIEREKLNLINSIYTTLEQFENYKNETIHFEQQRAINQVQQRVLQQALQGALGTLNSCLNNELHLRTIGANIDMFGAMKEKKN*