Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0268740 | KAF3776839.1 | 74.260 | 676 | 51 | 10 | 1 | 554 | 1 | 675 | 0.0 | 913 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0268740 | sp|Q2PMS8|ATPA_SOYBN | 79.765 | 425 | 7 | 5 | 209 | 554 | 7 | 431 | 0.0 | 637 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0268740 | A0A4S4EYF3 | 70.130 | 616 | 34 | 8 | 18 | 554 | 138 | 682 | 0.0 | 783 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002690 | Msa0268740 | 0.824631 | 7.270990e-54 | -8.615850e-47 |
| Msa0002700 | Msa0268740 | 0.826444 | 2.703391e-54 | -8.615850e-47 |
| Msa0016830 | Msa0268740 | 0.819311 | 1.241433e-52 | -8.615850e-47 |
| Msa0021570 | Msa0268740 | 0.824252 | 8.928623e-54 | -8.615850e-47 |
| Msa0028410 | Msa0268740 | 0.822648 | 2.116595e-53 | -8.615850e-47 |
| Msa0028450 | Msa0268740 | 0.805471 | 1.314049e-49 | -8.615850e-47 |
| Msa0030710 | Msa0268740 | 0.818562 | 1.837214e-52 | -8.615850e-47 |
| Msa0034800 | Msa0268740 | 0.801028 | 1.092521e-48 | -8.615850e-47 |
| Msa0054100 | Msa0268740 | 0.834777 | 2.463396e-56 | -8.615850e-47 |
| Msa0054110 | Msa0268740 | 0.807909 | 4.014252e-50 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0268740 | MtrunA17_Chr4g0015651 | 80.471 | 425 | 4 | 5 | 209 | 554 | 7 | 431 | 0.0 | 640 |
| Msa0268740 | MtrunA17_Chr4g0016741 | 80.471 | 425 | 4 | 5 | 209 | 554 | 7 | 431 | 0.0 | 640 |
| Msa0268740 | MtrunA17_CPg0492941 | 80.471 | 425 | 4 | 5 | 209 | 554 | 7 | 431 | 0.0 | 640 |
| Msa0268740 | MtrunA17_MTg0490471 | 54.042 | 433 | 111 | 6 | 210 | 554 | 9 | 441 | 1.38e-139 | 414 |
| Msa0268740 | MtrunA17_Chr2g0278821 | 84.656 | 189 | 15 | 2 | 380 | 554 | 24 | 212 | 1.42e-104 | 316 |
| Msa0268740 | MtrunA17_CPg0492961 | 94.118 | 119 | 2 | 1 | 95 | 208 | 49 | 167 | 7.09e-69 | 220 |
| Msa0268740 | MtrunA17_Chr2g0286451 | 65.574 | 183 | 8 | 2 | 271 | 398 | 7 | 189 | 2.19e-67 | 216 |
| Msa0268740 | MtrunA17_Chr4g0040631 | 88.034 | 117 | 4 | 1 | 209 | 315 | 7 | 123 | 9.17e-59 | 192 |
| Msa0268740 | MtrunA17_Chr4g0016711 | 98.765 | 81 | 1 | 0 | 1 | 81 | 1 | 81 | 4.21e-46 | 156 |
| Msa0268740 | MtrunA17_CPg0492971 | 98.765 | 81 | 1 | 0 | 1 | 81 | 1 | 81 | 4.21e-46 | 156 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0268740 | ATCG00120.1 | 77.647 | 425 | 16 | 5 | 209 | 554 | 7 | 431 | 0.0 | 620 |
| Msa0268740 | ATMG01190.1 | 51.039 | 433 | 124 | 6 | 210 | 554 | 9 | 441 | 1.90e-130 | 390 |
| Msa0268740 | AT2G07698.1 | 49.260 | 473 | 150 | 7 | 172 | 554 | 239 | 711 | 5.86e-129 | 395 |
| Msa0268740 | ATCG00130.1 | 78.689 | 122 | 21 | 1 | 93 | 209 | 49 | 170 | 1.00e-57 | 191 |
| Msa0268740 | ATCG00140.1 | 95.062 | 81 | 4 | 0 | 1 | 81 | 1 | 81 | 5.31e-44 | 151 |
| Msa0268740 | AT4G38510.5 | 27.794 | 349 | 166 | 10 | 227 | 502 | 50 | 385 | 3.88e-21 | 97.1 |
| Msa0268740 | AT4G38510.1 | 27.794 | 349 | 166 | 10 | 227 | 502 | 43 | 378 | 4.43e-21 | 97.1 |
| Msa0268740 | AT4G38510.3 | 27.794 | 349 | 166 | 10 | 227 | 502 | 43 | 378 | 4.43e-21 | 97.1 |
| Msa0268740 | AT4G38510.4 | 27.794 | 349 | 166 | 10 | 227 | 502 | 43 | 378 | 4.43e-21 | 97.1 |
| Msa0268740 | AT4G38510.2 | 27.794 | 349 | 166 | 10 | 227 | 502 | 43 | 378 | 4.43e-21 | 97.1 |
Find 125 sgRNAs with CRISPR-Local
Find 262 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGATTAAACAAAAGGAT+TGG | 0.459809 | 2_2:+70386737 | None:intergenic |
| AAAGCCATGAAACAGGTAGC+TGG | 0.558354 | 2_2:-70383722 | Msa0268740:intron |
| AAATATTTGGCCATCTGTAA+TGG | 0.434228 | 2_2:+70383832 | None:intergenic |
| AAATGTTGGTGTTGTATTAA+TGG | 0.258988 | 2_2:-70384632 | Msa0268740:CDS |
| AAATTTCAGCTTCGGAATCT+CGG | 0.492871 | 2_2:-70384480 | Msa0268740:intron |
| AAGCTGAAACATCTCCTGAT+TGG | 0.409535 | 2_2:+70383876 | None:intergenic |
| AAGGAAAGTAGAAACGGAAG+CGG | 0.622485 | 2_2:-70385227 | Msa0268740:CDS |
| AAGTAGAAACGGAAGCGGAT+CGG | 0.570689 | 2_2:-70385222 | Msa0268740:CDS |
| AATCAGACCAGCTATTAATG+TGG | 0.625394 | 2_2:-70383783 | Msa0268740:CDS |
| AATGTAATTTCCATTACAGA+TGG | 0.557336 | 2_2:-70383842 | Msa0268740:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CCGAATGAAGAATAAATTGT+AGG | + | chr2_2:70383543-70383562 | None:intergenic | 30.0% | |
| AACTAGTATCTACTTCTAAG+AGG | - | chr2_2:70383670-70383689 | Msa0268740:CDS | 30.0% | |
| ATAAATCTAAGTGTAGTGCT+AGG | - | chr2_2:70384045-70384064 | Msa0268740:CDS | 30.0% | |
| CAATTTCCGTATTAACATAG+TGG | + | chr2_2:70384361-70384380 | None:intergenic | 30.0% | |
| CACTATGTTAATACGGAAAT+TGG | - | chr2_2:70384359-70384378 | Msa0268740:CDS | 30.0% | |
| CGATGAAAAATAGGAAAAAC+AGG | - | chr2_2:70384435-70384454 | Msa0268740:intron | 30.0% | |
| TCAATTCCGCATTGTATAAA+AGG | + | chr2_2:70384478-70384497 | None:intergenic | 30.0% | |
| AGAAATAGAGAAAAGAGGAT+AGG | - | chr2_2:70384631-70384650 | Msa0268740:CDS | 30.0% | |
| GCTCATTCGATAATAAAGAT+AGG | - | chr2_2:70384653-70384672 | Msa0268740:CDS | 30.0% | |
| AGATAGGGAAATTTCCTATA+CGG | - | chr2_2:70384669-70384688 | Msa0268740:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_2 | gene | 70383160 | 70386988 | 70383160 | ID=Msa0268740;Name=Msa0268740 |
| chr2_2 | mRNA | 70383160 | 70386988 | 70383160 | ID=Msa0268740-mRNA-1;Parent=Msa0268740;Name=Msa0268740-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|0|0|0.9|0|0|10|0|556 |
| chr2_2 | exon | 70386749 | 70386988 | 70386749 | ID=Msa0268740-mRNA-1:exon:22658;Parent=Msa0268740-mRNA-1 |
| chr2_2 | exon | 70385209 | 70385395 | 70385209 | ID=Msa0268740-mRNA-1:exon:22657;Parent=Msa0268740-mRNA-1 |
| chr2_2 | exon | 70384996 | 70385193 | 70384996 | ID=Msa0268740-mRNA-1:exon:22656;Parent=Msa0268740-mRNA-1 |
| chr2_2 | exon | 70384743 | 70384844 | 70384743 | ID=Msa0268740-mRNA-1:exon:22655;Parent=Msa0268740-mRNA-1 |
| chr2_2 | exon | 70384481 | 70384712 | 70384481 | ID=Msa0268740-mRNA-1:exon:22654;Parent=Msa0268740-mRNA-1 |
| chr2_2 | exon | 70384266 | 70384444 | 70384266 | ID=Msa0268740-mRNA-1:exon:22653;Parent=Msa0268740-mRNA-1 |
| chr2_2 | exon | 70383936 | 70384136 | 70383936 | ID=Msa0268740-mRNA-1:exon:22652;Parent=Msa0268740-mRNA-1 |
| chr2_2 | exon | 70383766 | 70383929 | 70383766 | ID=Msa0268740-mRNA-1:exon:22651;Parent=Msa0268740-mRNA-1 |
| chr2_2 | exon | 70383574 | 70383729 | 70383574 | ID=Msa0268740-mRNA-1:exon:22650;Parent=Msa0268740-mRNA-1 |
| chr2_2 | exon | 70383160 | 70383171 | 70383160 | ID=Msa0268740-mRNA-1:exon:22649;Parent=Msa0268740-mRNA-1 |
| chr2_2 | CDS | 70386749 | 70386988 | 70386749 | ID=Msa0268740-mRNA-1:cds;Parent=Msa0268740-mRNA-1 |
| chr2_2 | CDS | 70385209 | 70385395 | 70385209 | ID=Msa0268740-mRNA-1:cds;Parent=Msa0268740-mRNA-1 |
| chr2_2 | CDS | 70384996 | 70385193 | 70384996 | ID=Msa0268740-mRNA-1:cds;Parent=Msa0268740-mRNA-1 |
| chr2_2 | CDS | 70384743 | 70384844 | 70384743 | ID=Msa0268740-mRNA-1:cds;Parent=Msa0268740-mRNA-1 |
| chr2_2 | CDS | 70384481 | 70384712 | 70384481 | ID=Msa0268740-mRNA-1:cds;Parent=Msa0268740-mRNA-1 |
| chr2_2 | CDS | 70384266 | 70384444 | 70384266 | ID=Msa0268740-mRNA-1:cds;Parent=Msa0268740-mRNA-1 |
| chr2_2 | CDS | 70383936 | 70384136 | 70383936 | ID=Msa0268740-mRNA-1:cds;Parent=Msa0268740-mRNA-1 |
| chr2_2 | CDS | 70383766 | 70383929 | 70383766 | ID=Msa0268740-mRNA-1:cds;Parent=Msa0268740-mRNA-1 |
| chr2_2 | CDS | 70383574 | 70383729 | 70383574 | ID=Msa0268740-mRNA-1:cds;Parent=Msa0268740-mRNA-1 |
| chr2_2 | CDS | 70383160 | 70383171 | 70383160 | ID=Msa0268740-mRNA-1:cds;Parent=Msa0268740-mRNA-1 |
| Gene Sequence |
>Msa0268740 ATGGATCCACTGATTTCTGCTGCTTCTGTTATCGCTGCTGGGTTGGCCGTCGGGCTTGCTTCTATTGGACCTGGAGTTGGTCAAGGTACAGCTGCAGGGCAAGCTGTAGAAGGGATTGCGCGACAACCGGAAGCAGAGGACAAAATAAGGGGTACTTTATTACTTAGTCTGGCTTTTATGGAAGCTTTAACTATTTATGGGCTGGTTGTAGCATTAGCACTTTTATTTGCCAATCCTTTTACATTTTTAAATAAATGCAAAGAGAATCTACTTTCATTAAGTGATTTATTAGATAATCGAAAACAGAGAATCTTGAGAACTATTCAAAATTCAGAAGAACTACGGGAAGCAGCCGTTGAACAGCTGGAGAAAGCCCGGGCCCGCTTAAGGAAAGTAGAAACGGAAGCGGATCGGTTTCGAGTGAATGAACGAGAAAAATTGAATTTAATTAATTCAATTTATACAACTTTGGAACAATTCGAAAATTACAAAAATGAAACGATTCATTTTGAACAACAAAGGGCGATTAATCAAGTGCAACAGAGGGTTTTACAACAAGCATTACAAGGAGCTCTGGGAACTCTGAATAGTTGCTTAAACAACGAGTTACATTTACGTACGATCGATGAAATTAGTAAAATTATCCGTGAACGTATTGAGCAATATAATACCGAAGTAAAAATTGTAAATACAGGTACTGTACTTCAAGTAGGCGACGGCATTGCTCGTGAATTAGTCGAATTTGAAGAGGGTACTGTAGGCATTGCTTTGAATTTGGAATCCAAAAATGTTGGTGTTGTATTAATGGGTGATGGTTTGCTGATACAAGAGGGAAGTTCGGTAAAAGCAACAGGAAGAATTGCTCAGATACCAGTAAGTGAGGGTTATTTGGGTCGTGTTGTAAATGCCCTAGCTAAACCTATTGATGGTCGAGGAGAAATTTCAGCTTCGGAATCTCGACGTTCCGTGTATGAGCCTCTTCAAACGGGACTTATTGCTATTGATTCTATGATCCCTATAGGGCGTGGCCAGCGAGAATTAATTATTGGGGACAGACAAACAGGTAAAACAGCAGTAGCAACAGATACAATTCTCAATCAACAGGGACAAAATGTAATATGTGTTTATGTAGCTATTGGTGCAGCTCTGGCTGAATATTTTATGTACCGTGAACGTCACACTTTAATCATTTATGACGATCCCTCCAAACAAGCACAGGCTTATCGCCAAATGTCTCTTCTATTACGAAGACCACCAGGTCGCGAAGCGTATCCCGGAGATGTTTTTTATTTACATTCTCGTCTTTTGGAAAGAGCCGCTAAATTAAGTTCTCAGTTAGGAAGTATGACTGCTTTACCAATAGTTGAGACCCAATCAGGAGATGTTTCAGCTTATATTCCTACTAATGTAATTTCCATTACAGATGGCCAAATATTTTTATCTGCCGATCTATTCAATGCTGGAATCAGACCAGCTATTAATGTGGGTATTTCCGTTTCCAGAGTAGCTGGTAAATTAAAATTGGAATTGGCACAATTCGCAGAATTAGAAGCTTTTGCACAATTCGCTTCTGATCTCGATAAAGCTACGCAAAATCAATTGGCAAGAGGTCAACGATTGCGCGAGTTGCTTAAACAATCCCAATCAGCTCCTCTCACTATACGAATATAA |
| Protein sequence |
>Msa0268740 MDPLISAASVIAAGLAVGLASIGPGVGQGTAAGQAVEGIARQPEAEDKIRGTLLLSLAFMEALTIYGLVVALALLFANPFTFLNKCKENLLSLSDLLDNRKQRILRTIQNSEELREAAVEQLEKARARLRKVETEADRFRVNEREKLNLINSIYTTLEQFENYKNETIHFEQQRAINQVQQRVLQQALQGALGTLNSCLNNELHLRTIDEISKIIRERIEQYNTEVKIVNTGTVLQVGDGIARELVEFEEGTVGIALNLESKNVGVVLMGDGLLIQEGSSVKATGRIAQIPVSEGYLGRVVNALAKPIDGRGEISASESRRSVYEPLQTGLIAIDSMIPIGRGQRELIIGDRQTGKTAVATDTILNQQGQNVICVYVAIGAALAEYFMYRERHTLIIYDDPSKQAQAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSSQLGSMTALPIVETQSGDVSAYIPTNVISITDGQIFLSADLFNAGIRPAINVGISVSRVAGKLKLELAQFAELEAFAQFASDLDKATQNQLARGQRLRELLKQSQSAPLTIRI* |
