AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1460130


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1460130 MtrunA17_CPg0492431 99.438 178 1 0 1 178 106 283 5.01e-129 363
Msa1460130 MtrunA17_Chr3g0090851 99.438 178 1 0 1 178 106 283 5.01e-129 363
Msa1460130 MtrunA17_Chr4g0016091 99.438 178 1 0 1 178 106 283 5.01e-129 363
Msa1460130 MtrunA17_Chr1g0195541 99.415 171 1 0 1 171 1 171 3.91e-123 344
Msa1460130 MtrunA17_Chr1g0153231 88.889 81 9 0 58 138 1 81 1.51e-46 147
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1460130 ATCG00890.1 97.794 136 3 0 1 136 1 136 1.74e-90 270
Msa1460130 ATCG01250.1 97.794 136 3 0 1 136 1 136 1.74e-90 270

Find 46 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGGTTTCTCTTGGCTATA+TGG 0.197078 tig0040677:+9814 Msa1460130:CDS
TGGGGCAAGCTCTTCTATTC+TGG 0.197255 tig0040677:+9789 Msa1460130:CDS
CTATCTATTTCTGAGATGTT+TGG 0.246287 tig0040677:+10036 Msa1460130:CDS
ATTAGACCGTACATCTTTCT+TGG 0.278852 tig0040677:-9737 None:intergenic
ATATTCATCACTGTAGGAAT+TGG 0.280413 tig0040677:+9928 Msa1460130:CDS
AGCTCTTCTATTCTGGTTCA+TGG 0.287942 tig0040677:+9796 Msa1460130:CDS
TTCTGGTTCATGGTTTCTCT+TGG 0.336946 tig0040677:+9806 Msa1460130:CDS
TTATGCTCCTATCTACTATC+TGG 0.367530 tig0040677:+9709 Msa1460130:CDS
CCCTAGCCCCTTCTCATCAA+TGG 0.393307 tig0040677:+9962 Msa1460130:CDS
TATTCATCACTGTAGGAATT+GGG 0.417349 tig0040677:+9929 Msa1460130:CDS
TCTTGGCTATATGGTTCGTC+CGG 0.418558 tig0040677:+9823 Msa1460130:CDS
CTTGGCTATATGGTTCGTCC+GGG 0.468938 tig0040677:+9824 Msa1460130:CDS
TACTATGAAATATTTACTCA+TGG 0.474204 tig0040677:+9765 Msa1460130:CDS
TAACAAGTAAAGTTATGTCT+TGG 0.481945 tig0040677:-10111 None:intergenic
GGTATATCCAGATAGTAGAT+AGG 0.488329 tig0040677:-9716 None:intergenic
ATAAGCGCAATTGAAATTCC+AGG 0.496050 tig0040677:-9907 None:intergenic
TGAAATATTTACTCATGGGT+GGG 0.506663 tig0040677:+9770 Msa1460130:CDS
CGCACTCCTTCATATACGTC+AGG 0.510042 tig0040677:-9988 None:intergenic
TCCATTGATGAGAAGGGGCT+AGG 0.510524 tig0040677:-9963 None:intergenic
TTGAAGCTCTATCTCTCCCC+CGG 0.517737 tig0040677:-9842 None:intergenic
ACACAAATGTATAACTCCCC+TGG 0.530663 tig0040677:+9889 Msa1460130:CDS
ATGAAATATTTACTCATGGG+TGG 0.533610 tig0040677:+9769 Msa1460130:CDS
GAAATATTTACTCATGGGTG+GGG 0.537789 tig0040677:+9771 Msa1460130:CDS
CAGGAGTCCATTGATGAGAA+GGG 0.542754 tig0040677:-9969 None:intergenic
GAGCTTCAAGAAATAGTGAA+TGG 0.546870 tig0040677:+9856 Msa1460130:CDS
TCAGGAGTCCATTGATGAGA+AGG 0.551074 tig0040677:-9970 None:intergenic
CCATTGATGAGAAGGGGCTA+GGG 0.552174 tig0040677:-9962 None:intergenic
TTCATATTAACCGCTACTCT+AGG 0.552302 tig0040677:+9622 Msa1460130:CDS
GAAAGATGTACGGTCTAATG+AGG 0.565206 tig0040677:+9741 Msa1460130:CDS
TAAGCGCAATTGAAATTCCA+GGG 0.565360 tig0040677:-9906 None:intergenic
TGGCTATATGGTTCGTCCGG+GGG 0.574450 tig0040677:+9826 Msa1460130:CDS
TTGGCTATATGGTTCGTCCG+GGG 0.582473 tig0040677:+9825 Msa1460130:CDS
ACTATGAAATATTTACTCAT+GGG 0.584827 tig0040677:+9766 Msa1460130:CDS
TGGACTCCTGACGTATATGA+AGG 0.588163 tig0040677:+9982 Msa1460130:CDS
CATCTCAGAAATAGATAGAG+AGG 0.593972 tig0040677:-10031 None:intergenic
GCGCTTATATTCATCACTGT+AGG 0.594447 tig0040677:+9922 Msa1460130:CDS
ATATTAACCGCTACTCTAGG+AGG 0.609728 tig0040677:+9625 Msa1460130:CDS
AGTTATGTCTTGGTCCGAGT+GGG 0.616525 tig0040677:-10101 None:intergenic
GATATACCAAGAAAGATGTA+CGG 0.625842 tig0040677:+9731 Msa1460130:CDS
AGGAGTCCATTGATGAGAAG+GGG 0.632944 tig0040677:-9968 None:intergenic
TCAGAAATAGATAGAGAGGT+CGG 0.633834 tig0040677:-10027 None:intergenic
AAGTTATGTCTTGGTCCGAG+TGG 0.648471 tig0040677:-10102 None:intergenic
AAACATTCCTCCTAGAGTAG+CGG 0.663526 tig0040677:-9632 None:intergenic
AGAGACATGCTATTCCCACT+CGG 0.691777 tig0040677:+10087 Msa1460130:CDS
CTGACGTATATGAAGGAGTG+CGG 0.702764 tig0040677:+9989 Msa1460130:CDS
AAGCGCAATTGAAATTCCAG+GGG 0.720091 tig0040677:-9905 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTATGAAATATTTACTCA+TGG + tig0040677:9765-9784 Msa1460130:CDS 20.0%
!! ACTATGAAATATTTACTCAT+GGG + tig0040677:9766-9785 Msa1460130:CDS 20.0%
!!! TTGGATTTTTCAAAACTCTA+TGG + tig0040677:10055-10074 Msa1460130:CDS 25.0%
! TAACAAGTAAAGTTATGTCT+TGG - tig0040677:10114-10133 None:intergenic 25.0%
GATATACCAAGAAAGATGTA+CGG + tig0040677:9731-9750 Msa1460130:CDS 30.0%
ATGAAATATTTACTCATGGG+TGG + tig0040677:9769-9788 Msa1460130:CDS 30.0%
TGAAATATTTACTCATGGGT+GGG + tig0040677:9770-9789 Msa1460130:CDS 30.0%
ATATTCATCACTGTAGGAAT+TGG + tig0040677:9928-9947 Msa1460130:CDS 30.0%
TATTCATCACTGTAGGAATT+GGG + tig0040677:9929-9948 Msa1460130:CDS 30.0%
!! CTATCTATTTCTGAGATGTT+TGG + tig0040677:10036-10055 Msa1460130:CDS 30.0%
TTCATATTAACCGCTACTCT+AGG + tig0040677:9622-9641 Msa1460130:CDS 35.0%
TTATGCTCCTATCTACTATC+TGG + tig0040677:9709-9728 Msa1460130:CDS 35.0%
GGTATATCCAGATAGTAGAT+AGG - tig0040677:9719-9738 None:intergenic 35.0%
ATTAGACCGTACATCTTTCT+TGG - tig0040677:9740-9759 None:intergenic 35.0%
GAAATATTTACTCATGGGTG+GGG + tig0040677:9771-9790 Msa1460130:CDS 35.0%
GAGCTTCAAGAAATAGTGAA+TGG + tig0040677:9856-9875 Msa1460130:CDS 35.0%
TAAGCGCAATTGAAATTCCA+GGG - tig0040677:9909-9928 None:intergenic 35.0%
ATAAGCGCAATTGAAATTCC+AGG - tig0040677:9910-9929 None:intergenic 35.0%
TCAGAAATAGATAGAGAGGT+CGG - tig0040677:10030-10049 None:intergenic 35.0%
CATCTCAGAAATAGATAGAG+AGG - tig0040677:10034-10053 None:intergenic 35.0%
ATATTAACCGCTACTCTAGG+AGG + tig0040677:9625-9644 Msa1460130:CDS 40.0%
AAACATTCCTCCTAGAGTAG+CGG - tig0040677:9635-9654 None:intergenic 40.0%
GAAAGATGTACGGTCTAATG+AGG + tig0040677:9741-9760 Msa1460130:CDS 40.0%
!! AGCTCTTCTATTCTGGTTCA+TGG + tig0040677:9796-9815 Msa1460130:CDS 40.0%
!! TTCTGGTTCATGGTTTCTCT+TGG + tig0040677:9806-9825 Msa1460130:CDS 40.0%
CATGGTTTCTCTTGGCTATA+TGG + tig0040677:9814-9833 Msa1460130:CDS 40.0%
ACACAAATGTATAACTCCCC+TGG + tig0040677:9889-9908 Msa1460130:CDS 40.0%
AAGCGCAATTGAAATTCCAG+GGG - tig0040677:9908-9927 None:intergenic 40.0%
GCGCTTATATTCATCACTGT+AGG + tig0040677:9922-9941 Msa1460130:CDS 40.0%
TCTTGGCTATATGGTTCGTC+CGG + tig0040677:9823-9842 Msa1460130:CDS 45.0%
AGGAGTCCATTGATGAGAAG+GGG - tig0040677:9971-9990 None:intergenic 45.0%
CAGGAGTCCATTGATGAGAA+GGG - tig0040677:9972-9991 None:intergenic 45.0%
TCAGGAGTCCATTGATGAGA+AGG - tig0040677:9973-9992 None:intergenic 45.0%
TGGACTCCTGACGTATATGA+AGG + tig0040677:9982-10001 Msa1460130:CDS 45.0%
CTGACGTATATGAAGGAGTG+CGG + tig0040677:9989-10008 Msa1460130:CDS 45.0%
AGAGACATGCTATTCCCACT+CGG + tig0040677:10087-10106 Msa1460130:CDS 45.0%
AGTTATGTCTTGGTCCGAGT+GGG - tig0040677:10104-10123 None:intergenic 45.0%
AAGTTATGTCTTGGTCCGAG+TGG - tig0040677:10105-10124 None:intergenic 45.0%
TGGGGCAAGCTCTTCTATTC+TGG + tig0040677:9789-9808 Msa1460130:CDS 50.0%
CTTGGCTATATGGTTCGTCC+GGG + tig0040677:9824-9843 Msa1460130:CDS 50.0%
TTGGCTATATGGTTCGTCCG+GGG + tig0040677:9825-9844 Msa1460130:CDS 50.0%
TTGAAGCTCTATCTCTCCCC+CGG - tig0040677:9845-9864 None:intergenic 50.0%
CCATTGATGAGAAGGGGCTA+GGG - tig0040677:9965-9984 None:intergenic 50.0%
TCCATTGATGAGAAGGGGCT+AGG - tig0040677:9966-9985 None:intergenic 50.0%
CGCACTCCTTCATATACGTC+AGG - tig0040677:9991-10010 None:intergenic 50.0%
TGGCTATATGGTTCGTCCGG+GGG + tig0040677:9826-9845 Msa1460130:CDS 55.0%
CCCTAGCCCCTTCTCATCAA+TGG + tig0040677:9962-9981 Msa1460130:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0040677 gene 9598 10134 9598 ID=Msa1460130;Name=Msa1460130
tig0040677 mRNA 9598 10134 9598 ID=Msa1460130-mRNA-1;Parent=Msa1460130;Name=Msa1460130-mRNA-1;_AED=0.58;_eAED=0.58;_QI=0|-1|0|1|-1|1|1|0|178
tig0040677 exon 9598 10134 9598 ID=Msa1460130-mRNA-1:exon:22582;Parent=Msa1460130-mRNA-1
tig0040677 CDS 9598 10134 9598 ID=Msa1460130-mRNA-1:cds;Parent=Msa1460130-mRNA-1
Gene Sequence

>Msa1460130

ATGGCTATAACAGAGTTTCTATTATTCATATTAACCGCTACTCTAGGAGGAATGTTTTTATGCAGCGCTAATGATTTAATAACTATCTTTGTAGCTCTAGAATGTTTCAGTTTATGCTCCTATCTACTATCTGGATATACCAAGAAAGATGTACGGTCTAATGAGGCTACTATGAAATATTTACTCATGGGTGGGGCAAGCTCTTCTATTCTGGTTCATGGTTTCTCTTGGCTATATGGTTCGTCCGGGGGAGAGATAGAGCTTCAAGAAATAGTGAATGGTCTTATCAATACACAAATGTATAACTCCCCTGGAATTTCAATTGCGCTTATATTCATCACTGTAGGAATTGGGTTCAAGCTTTCCCTAGCCCCTTCTCATCAATGGACTCCTGACGTATATGAAGGAGTGCGGTTCGTTCGAGAAATTCCGACCTCTCTATCTATTTCTGAGATGTTTGGATTTTTCAAAACTCTATGGACATGCAGAAGAGACATGCTATTCCCACTCGGACCAAGACATAACTTTACTTGTTAA

Protein sequence

>Msa1460130

MAITEFLLFILTATLGGMFLCSANDLITIFVALECFSLCSYLLSGYTKKDVRSNEATMKYLLMGGASSSILVHGFSWLYGSSGGEIELQEIVNGLINTQMYNSPGISIALIFITVGIGFKLSLAPSHQWTPDVYEGVRFVREIPTSLSISEMFGFFKTLWTCRRDMLFPLGPRHNFTC*