AlfalfaGEDB | Msa1016320

M. sativa cultivar zhongmu-4 / Msa1016320

KEGG: -
GO: -
PFAM: PF01107.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1016320	RHN45423.1	90.583	223	20	1	4	226	5	226	8.62e-141	403

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1016320	A0A396GY75	90.583	223	20	1	4	226	5	226	4.12e-141	403

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003890	Msa1016320	0.824816	6.575377e-54	-8.615850e-47
Msa0006070	Msa1016320	0.811723	6.069885e-51	-8.615850e-47
Msa0006110	Msa1016320	0.854312	1.338702e-61	-8.615850e-47
Msa0007990	Msa1016320	0.819334	1.226729e-52	-8.615850e-47
Msa0011170	Msa1016320	0.828632	8.071851e-55	-8.615850e-47
Msa0011660	Msa1016320	-0.816186	6.296470e-52	-8.615850e-47
Msa0015390	Msa1016320	0.829816	4.166273e-55	-8.615850e-47
Msa0017510	Msa1016320	0.835951	1.244396e-56	-8.615850e-47
Msa0018650	Msa1016320	0.804088	2.555427e-49	-8.615850e-47
Msa0020430	Msa1016320	0.805691	1.181151e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1016320	MtrunA17_Chr7g0231021	90.583	223	20	1	4	226	5	226	7.93e-145	403

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 24 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTTGAAGATTCAGTCT+TGG	0.484593	7_1:+32461483	Msa1016320:CDS
AGAGTTAAGTGTATAATCAA+TGG	0.469570	7_1:+32461782	Msa1016320:three_prime_UTR
AGATTCAGTCTTGGTACGTT+TGG	0.383413	7_1:+32461492	Msa1016320:CDS
AGCATCTATAGTCACAACCA+AGG	0.708480	7_1:-32461599	None:intergenic
AGGAAGAAATCTATTAAACT+TGG	0.468019	7_1:+32461673	Msa1016320:CDS
AGGGTGACATAAAGTTTAAT+TGG	0.341843	7_1:+32461530	Msa1016320:CDS
ATAGGGTTTGTTATAAATTA+AGG	0.230557	7_1:+32461653	Msa1016320:CDS
ATCTTTAAAAGTTAGGTATA+GGG	0.395637	7_1:+32461636	Msa1016320:CDS
ATGAAGAAATATGCTGAATT+TGG	0.243744	7_1:+32461843	Msa1016320:three_prime_UTR
CAATTGTGAAATGTAAATTC+TGG	0.337215	7_1:-32462018	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTACAAGCCAAGTACAAA+AGG	+	chr7_1:32458801-32458820	Msa1016320:intron	30.0%
TACCAAGTTGCACAAATTAT+TGG	-	chr7_1:32458836-32458855	None:intergenic	30.0%
TTTGGTCCTTAAAATGCTTA+AGG	+	chr7_1:32459033-32459052	Msa1016320:intron	30.0%
ATATCCAGGTCTTAAAATCT+CGG	-	chr7_1:32459151-32459170	None:intergenic	30.0%
CTTTCCAATTACGATCATAA+AGG	-	chr7_1:32459384-32459403	None:intergenic	30.0%
CTTTATGATCGTAATTGGAA+AGG	+	chr7_1:32459382-32459401	Msa1016320:intron	30.0%
GATTATGAATTGGAATGTAG+AGG	+	chr7_1:32459422-32459441	Msa1016320:intron	30.0%
CAATTGTCTGATAAGTTCAA+GGG	+	chr7_1:32459499-32459518	Msa1016320:intron	30.0%
GCAATGATTAATTGAGCTTA+GGG	+	chr7_1:32459586-32459605	Msa1016320:intron	30.0%
ATGCTAGAATTAACGATTAC+AGG	+	chr7_1:32459645-32459664	Msa1016320:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_1	gene	32458779	32462079	32458779	ID=Msa1016320;Name=Msa1016320
chr7_1	mRNA	32458779	32462079	32458779	ID=Msa1016320-mRNA-1;Parent=Msa1016320;Name=Msa1016320-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0\|0\|0.5\|0.5\|0\|0.5\|2\|317\|226
chr7_1	exon	32458779	32458784	32458779	ID=Msa1016320-mRNA-1:exon:6400;Parent=Msa1016320-mRNA-1
chr7_1	exon	32461088	32462079	32461088	ID=Msa1016320-mRNA-1:exon:6401;Parent=Msa1016320-mRNA-1
chr7_1	CDS	32458779	32458784	32458779	ID=Msa1016320-mRNA-1:cds;Parent=Msa1016320-mRNA-1
chr7_1	CDS	32461088	32461762	32461088	ID=Msa1016320-mRNA-1:cds;Parent=Msa1016320-mRNA-1
chr7_1	three_prime_UTR	32461763	32462079	32461763	ID=Msa1016320-mRNA-1:three_prime_utr;Parent=Msa1016320-mRNA-1

Gene Sequence

>Msa1016320

ATGAGAGGACAAGATCATCAATCATCACTCCGAATGGATTCTTTTTATTTTGTTCCTGCTGAGAACACAATTGATGATAGTGAAAAGGAATACAGTATTGTTCACTTCCAATATTCCAACAACAACGTCATAAAAAAAATATCAACTAGTCAAATTTATAAGCAAACACTATTTTCAAAATTCAATTTTCTAAAACCTTACAAAATTACAACAATAGAAGAAAATCTTTCAGCAGAGAAATACAAAGATGTGTATTTGCTATCAAGAGATGACATTAGAAGTCATAGAAGTAACTATAATTTTCTTCATATTGGGTTGGTACAATTTTCTATAGTTAATAGCTATCGAATTTCACAAGAAACACTTAATGTGTCAATAAGTTTGAGAGATTCTAAGTACCAAAAATTTGAAGATTCAGTCTTGGTACGTTTGGATTCAGATTTGTGTAAGGGTGACATAAAGTTTAATTGGTTTCCAAATTTTTCGGCACGTTTGTCTGATCTAGCGAATTCGAACGCCTTGGTTGTGACTATAGATGCTCCAGATTGTCGTCGATCTTTAAAAGTTAGGTATAGGGTTTGTTATAAATTAAGGAAGAAATCTATTAAACTTGGTTATCTATTTAAAAATCCTATGGTAGAGGTGGACACTGAGAAAACAAATGTTGTTATCCCCAGATAA

Protein sequence

>Msa1016320

MRGQDHQSSLRMDSFYFVPAENTIDDSEKEYSIVHFQYSNNNVIKKISTSQIYKQTLFSKFNFLKPYKITTIEENLSAEKYKDVYLLSRDDIRSHRSNYNFLHIGLVQFSIVNSYRISQETLNVSISLRDSKYQKFEDSVLVRLDSDLCKGDIKFNWFPNFSARLSDLANSNALVVTIDAPDCRRSLKVRYRVCYKLRKKSIKLGYLFKNPMVEVDTEKTNVVIPR*