AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0867610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0867610 MtrunA17_Chr2g0320231 92.357 314 12 2 1 308 1 308 0.0 570
Msa0867610 MtrunA17_Chr8g0379121 64.493 138 43 2 32 167 48 181 2.38e-45 159
Msa0867610 MtrunA17_Chr2g0283951 56.410 156 50 5 35 183 37 181 5.37e-43 151
Msa0867610 MtrunA17_Chr7g0243081 66.667 111 36 1 52 162 27 136 6.29e-42 148
Msa0867610 MtrunA17_Chr1g0162931 69.737 76 23 0 84 159 5 80 1.74e-31 119
Msa0867610 MtrunA17_Chr1g0210911 60.000 90 33 2 83 169 8 97 1.90e-30 117
Msa0867610 MtrunA17_Chr2g0288601 64.865 74 26 0 75 148 49 122 1.12e-29 109
Msa0867610 MtrunA17_Chr3g0095611 55.696 79 29 1 75 153 3 75 2.75e-23 95.5
Msa0867610 MtrunA17_Chr1g0154261 64.789 71 22 2 89 158 120 188 1.57e-21 92.4
Msa0867610 MtrunA17_Chr6g0479431 68.966 58 18 0 96 153 201 258 1.35e-20 90.9
Msa0867610 MtrunA17_Chr5g0410741 68.966 58 18 0 96 153 218 275 2.97e-20 90.1
Msa0867610 MtrunA17_Chr3g0109981 68.966 58 18 0 96 153 273 330 4.04e-20 90.5
Msa0867610 MtrunA17_Chr8g0366281 63.934 61 22 0 96 156 172 232 7.27e-20 88.6
Msa0867610 MtrunA17_Chr7g0261911 67.857 56 18 0 98 153 263 318 1.82e-19 88.6
Msa0867610 MtrunA17_Chr7g0274471 49.425 87 43 1 98 183 219 305 4.01e-19 87.4
Msa0867610 MtrunA17_Chr1g0190301 61.194 67 25 1 87 153 166 231 4.63e-19 86.7
Msa0867610 MtrunA17_Chr7g0258551 50.000 96 40 3 93 187 208 296 4.92e-19 86.7
Msa0867610 MtrunA17_Chr1g0175091 50.000 96 39 3 70 164 239 326 3.32e-17 81.6
Msa0867610 MtrunA17_Chr6g0461641 62.264 53 20 0 98 150 43 95 6.45e-17 80.5
Msa0867610 MtrunA17_Chr5g0409731 60.345 58 23 0 93 150 21 78 6.48e-17 79.3
Msa0867610 MtrunA17_Chr2g0321461 58.929 56 23 0 98 153 46 101 7.61e-17 80.5
Msa0867610 MtrunA17_Chr7g0254981 65.000 60 20 1 99 157 52 111 2.43e-16 77.8
Msa0867610 MtrunA17_Chr2g0290991 42.857 105 50 2 98 202 40 134 3.19e-16 77.8
Msa0867610 MtrunA17_Chr1g0197171 60.714 56 22 0 98 153 45 100 3.82e-16 77.4
Msa0867610 MtrunA17_Chr1g0175081 54.839 62 27 1 99 159 24 85 4.00e-16 72.8
Msa0867610 MtrunA17_Chr2g0291021 65.455 55 19 0 99 153 51 105 1.59e-15 75.9
Msa0867610 MtrunA17_Chr5g0417521 52.830 53 25 0 98 150 16 68 4.02e-15 75.1
Msa0867610 MtrunA17_Chr8g0356501 55.357 56 25 0 98 153 38 93 9.65e-15 73.9
Msa0867610 MtrunA17_Chr2g0310011 55.357 56 25 0 98 153 36 91 2.30e-14 72.8
Msa0867610 MtrunA17_Chr4g0042451 52.830 53 25 0 98 150 18 70 1.21e-13 70.5
Msa0867610 MtrunA17_Chr1g0175071 58.491 53 22 0 99 151 179 231 1.43e-13 70.1
Msa0867610 MtrunA17_Chr7g0242521 61.017 59 20 1 99 154 15 73 1.79e-13 68.9
Msa0867610 MtrunA17_Chr7g0258531 58.491 53 22 0 99 151 215 267 7.52e-12 65.5
Msa0867610 MtrunA17_Chr4g0009901 43.243 74 38 2 80 150 148 220 8.31e-12 64.7
Msa0867610 MtrunA17_Chr4g0062921 39.130 92 48 1 67 150 197 288 1.42e-11 64.7
Msa0867610 MtrunA17_Chr4g0045361 45.455 66 36 0 85 150 262 327 2.17e-11 64.3
Msa0867610 MtrunA17_Chr4g0031961 53.125 64 29 1 87 150 121 183 2.37e-11 63.5
Msa0867610 MtrunA17_Chr2g0304841 52.727 55 25 1 96 150 212 265 2.53e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0867610 AT2G38300.1 73.256 86 23 0 77 162 34 119 5.80e-38 137
Msa0867610 AT2G42660.1 67.021 94 20 2 89 177 42 129 9.40e-35 127
Msa0867610 AT2G40260.1 75.325 77 19 0 80 156 65 141 4.03e-33 126
Msa0867610 AT4G04605.1 65.882 85 27 1 89 171 6 90 1.55e-31 117
Msa0867610 AT2G02060.2 67.059 85 21 2 66 150 5 82 2.13e-28 110
Msa0867610 AT2G02060.1 67.059 85 21 2 66 150 5 82 2.13e-28 110
Msa0867610 AT1G14600.1 67.606 71 23 0 89 159 15 85 1.73e-27 108
Msa0867610 AT4G04555.1 61.644 73 28 0 85 157 2 74 3.59e-25 97.4
Msa0867610 AT4G04580.1 60.870 69 27 0 89 157 6 74 7.91e-24 95.9
Msa0867610 AT5G42630.2 65.079 63 22 0 96 158 104 166 7.03e-22 92.0
Msa0867610 AT5G42630.1 65.079 63 22 0 96 158 104 166 1.21e-21 92.8
Msa0867610 AT1G32240.2 68.966 58 18 0 96 153 212 269 2.78e-20 90.5
Msa0867610 AT4G17695.1 62.319 69 24 1 88 156 157 223 3.16e-20 89.7
Msa0867610 AT1G32240.1 68.966 58 18 0 96 153 212 269 3.54e-20 90.5
Msa0867610 AT5G16560.1 68.966 58 18 0 96 153 218 275 4.82e-20 90.1
Msa0867610 AT5G29000.4 36.250 160 89 5 5 153 130 287 9.03e-20 88.2
Msa0867610 AT5G29000.2 36.250 160 89 6 5 153 130 287 4.15e-19 87.4
Msa0867610 AT5G29000.1 36.250 160 89 6 5 153 87 244 4.58e-19 87.0
Msa0867610 AT5G29000.3 36.250 160 89 6 5 153 87 244 4.58e-19 87.0
Msa0867610 AT4G28610.1 48.276 87 43 1 99 183 227 313 6.73e-19 86.7
Msa0867610 AT5G45580.1 47.191 89 42 1 93 181 3 86 1.81e-18 83.6
Msa0867610 AT5G45580.2 47.191 89 42 1 93 181 19 102 2.57e-18 83.6
Msa0867610 AT3G04450.3 52.778 72 29 1 99 165 240 311 6.38e-18 83.2
Msa0867610 AT3G04450.4 61.818 55 21 0 99 153 200 254 2.82e-17 82.0
Msa0867610 AT3G04450.2 61.818 55 21 0 99 153 200 254 2.82e-17 82.0
Msa0867610 AT3G04450.1 61.818 55 21 0 99 153 240 294 2.95e-17 82.4
Msa0867610 AT3G13040.3 67.273 55 18 0 99 153 243 297 1.14e-16 80.5
Msa0867610 AT3G13040.1 67.273 55 18 0 99 153 243 297 1.14e-16 80.5
Msa0867610 AT3G13040.2 67.273 55 18 0 99 153 243 297 1.14e-16 80.5
Msa0867610 AT5G18240.2 47.126 87 43 2 82 167 30 114 1.52e-16 79.7
Msa0867610 AT5G18240.3 47.126 87 43 2 82 167 30 114 1.52e-16 79.7
Msa0867610 AT5G18240.1 43.269 104 56 2 65 167 13 114 1.91e-16 79.7
Msa0867610 AT5G18240.4 43.269 104 56 2 65 167 13 114 1.91e-16 79.7
Msa0867610 AT5G18240.5 43.269 104 56 2 65 167 13 114 1.95e-16 79.7
Msa0867610 AT2G06020.1 47.561 82 40 1 80 158 66 147 4.16e-16 77.8
Msa0867610 AT3G04030.1 55.932 59 25 1 98 155 46 104 5.80e-16 78.2
Msa0867610 AT3G04030.3 55.932 59 25 1 98 155 46 104 6.21e-16 78.2
Msa0867610 AT2G01060.1 55.844 77 21 2 99 162 17 93 1.11e-15 76.3
Msa0867610 AT3G12730.1 36.885 122 60 3 98 213 24 134 1.32e-15 75.1
Msa0867610 AT2G20400.2 63.636 55 20 0 99 153 233 287 3.28e-15 75.9
Msa0867610 AT2G20400.1 63.636 55 20 0 99 153 233 287 3.39e-15 75.9
Msa0867610 AT5G06800.3 56.452 62 26 1 99 159 194 255 3.42e-15 74.7
Msa0867610 AT3G24120.1 56.604 53 23 0 98 150 42 94 8.39e-15 73.9
Msa0867610 AT3G24120.2 56.604 53 23 0 98 150 42 94 8.81e-15 73.9
Msa0867610 AT4G13640.2 56.604 53 23 0 98 150 38 90 8.97e-15 73.9
Msa0867610 AT4G13640.1 56.604 53 23 0 98 150 38 90 9.04e-15 73.9
Msa0867610 AT5G06800.2 56.452 62 26 1 99 159 194 255 1.62e-14 73.9
Msa0867610 AT5G06800.1 60.000 55 22 0 99 153 194 248 1.66e-14 73.9
Msa0867610 AT1G79430.2 55.357 56 25 0 98 153 35 90 1.70e-14 73.6
Msa0867610 AT1G69580.1 34.711 121 73 3 98 213 35 154 2.73e-14 72.8
Msa0867610 AT1G69580.2 40.426 94 51 2 98 186 35 128 5.03e-14 72.0
Msa0867610 AT3G04030.2 54.237 59 25 2 98 155 46 103 1.52e-13 70.9
Msa0867610 AT5G59570.2 57.143 56 23 1 95 150 139 193 1.22e-11 64.7
Msa0867610 AT5G59570.1 57.143 56 23 1 95 150 139 193 1.22e-11 64.7
Msa0867610 AT2G03500.1 50.746 67 31 1 84 150 222 286 2.50e-11 64.3
Msa0867610 AT3G62670.1 44.872 78 38 2 78 150 191 268 2.71e-11 64.3
Msa0867610 AT4G31920.1 40.000 105 61 2 85 189 171 273 3.58e-11 64.3
Msa0867610 AT3G16857.3 43.158 95 49 3 60 150 126 219 4.27e-11 63.9
Msa0867610 AT3G16857.2 43.158 95 49 3 60 150 196 289 5.25e-11 63.9
Msa0867610 AT3G46640.2 56.364 55 23 1 96 150 143 196 5.44e-11 63.2
Msa0867610 AT3G46640.1 56.364 55 23 1 96 150 143 196 5.44e-11 63.2
Msa0867610 AT4G37180.1 50.847 59 29 0 92 150 205 263 6.15e-11 63.2
Msa0867610 AT3G16857.1 43.158 95 49 3 60 150 196 289 6.34e-11 63.5
Msa0867610 AT3G46640.3 56.364 55 23 1 96 150 143 196 7.29e-11 62.8
Msa0867610 AT4G37180.2 50.847 59 29 0 92 150 212 270 7.31e-11 62.8
Msa0867610 AT5G05090.1 52.000 75 33 3 77 150 61 133 7.48e-11 62.0

Find 70 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACAAGGTTTAATAAATTT+TGG 0.129635 6_1:+608120 Msa0867610:five_prime_UTR
AGATATGTTAGTTAAATATT+TGG 0.161045 6_1:-608221 None:intergenic
TTTATAAACAAGTATCTTAT+AGG 0.188196 6_1:+608021 Msa0867610:five_prime_UTR
GAAATTTGAACATAAATTAA+TGG 0.280764 6_1:+608340 Msa0867610:exon
AATATAACTATCGTATTATA+TGG 0.284509 6_1:-608249 None:intergenic
CTTACTTGGCTAGACCTAAT+AGG 0.308735 6_1:-610667 None:intergenic
GATATGTTAGTTAAATATTT+GGG 0.317333 6_1:-608220 None:intergenic
TTCACTGAATACACAATGTT+TGG 0.328197 6_1:+610808 Msa0867610:CDS
AATATTTAACTAACATATCT+TGG 0.346553 6_1:+608224 Msa0867610:five_prime_UTR
GCAAGGAAGGCATCACATTA+TGG 0.348737 6_1:+609982 Msa0867610:CDS
GTCCTCTCACATTCATTAAC+TGG 0.352977 6_1:-609315 None:intergenic
CATTAACTGGAGAACTAACT+TGG 0.357662 6_1:-609302 None:intergenic
CGGGTAGTAATTGACTTACT+TGG 0.374141 6_1:-610681 None:intergenic
TTCACATCTATTTGATGTAA+AGG 0.385780 6_1:+610618 Msa0867610:CDS
TCAAAGCTCATGACACTTCA+AGG 0.388453 6_1:+610086 Msa0867610:CDS
ACATGTGAAGAGTCATTTGC+AGG 0.413814 6_1:+609348 Msa0867610:CDS
GAAATAATATTGCACCTATT+AGG 0.414171 6_1:+610653 Msa0867610:CDS
AATAATAATTCTAGTGAAGA+AGG 0.443311 6_1:+608581 Msa0867610:CDS
TTATCTCAAGGGGATGATAA+TGG 0.444178 6_1:+610715 Msa0867610:CDS
TTGTGCTTGTTCCTAAGTCT+TGG 0.452034 6_1:-608103 None:intergenic
GTGCATGCTGTTGAGAGGCT+TGG 0.454830 6_1:+608689 Msa0867610:CDS
CATCACATTATGGATATGTA+TGG 0.459114 6_1:+609992 Msa0867610:CDS
ACAAAAGATAAATGAAGATC+AGG 0.462114 6_1:-608668 None:intergenic
AGTGAAAGTAGTAGCGGTAT+CGG 0.469081 6_1:+608395 Msa0867610:CDS
CTTCGAATCGGTAAAGAGGC+AGG 0.473218 6_1:-608425 None:intergenic
AATTCTAGTGAAGAAGGAAA+AGG 0.475804 6_1:+608587 Msa0867610:CDS
GAAGAATTACAAACTTATGT+TGG 0.479251 6_1:+608272 Msa0867610:five_prime_UTR
TTTGTAGATTTCATGAGAGT+GGG 0.483163 6_1:+610539 Msa0867610:intron
CATGCTGTTGAGAGGCTTGG+AGG 0.487742 6_1:+608692 Msa0867610:CDS
CGAAAATGCCTAGGCTTCGT+TGG 0.491348 6_1:+608642 Msa0867610:CDS
TTCAAATTGTAAGTTTACAT+AGG 0.499050 6_1:+608071 Msa0867610:five_prime_UTR
ATAATTGATAGAACTAGAAA+AGG 0.502223 6_1:-610761 None:intergenic
ATTCTAGTGAAGAAGGAAAA+GGG 0.515576 6_1:+608588 Msa0867610:CDS
ATTGTGTATTCAGTGAAGAT+AGG 0.520662 6_1:-610802 None:intergenic
AACGAAGAAGCCGTGGACGA+TGG 0.526235 6_1:+608470 Msa0867610:CDS
AGGAAAAGGGCGTGGAAGTA+CGG 0.529396 6_1:+608601 Msa0867610:CDS
ATACACAATGTTTGGTTCTG+AGG 0.536912 6_1:+610816 Msa0867610:CDS
AAAATAGAGCAATGCAAGGA+AGG 0.540544 6_1:+609969 Msa0867610:CDS
TATGATGGTTGTTTCAGTAG+AGG 0.540634 6_1:-610055 None:intergenic
GAACTAGAAAAGGTGCTCCA+TGG 0.547694 6_1:-610751 None:intergenic
ATTGTAAGTTTACATAGGTG+TGG 0.549908 6_1:+608076 Msa0867610:five_prime_UTR
TCACTCTTGACATCTTTGCT+AGG 0.551418 6_1:-608377 None:intergenic
GCTTTGATTTCATTATATGA+TGG 0.554601 6_1:-610070 None:intergenic
TCAGGAGTCCAACGAAGCCT+AGG 0.559435 6_1:-608650 None:intergenic
AATGAGATATCATCACAAGA+AGG 0.560760 6_1:+608545 Msa0867610:CDS
AAGGGCGTGGAAGTACGGTT+AGG 0.570454 6_1:+608606 Msa0867610:CDS
GGTGTGGAATTCCAAGACTT+AGG 0.582021 6_1:+608092 Msa0867610:five_prime_UTR
ATGAGATATCATCACAAGAA+GGG 0.586610 6_1:+608546 Msa0867610:CDS
ATGGATATGTATGGACACTT+TGG 0.587338 6_1:+610001 Msa0867610:CDS
AGTGAAGAAGGAAAAGGGCG+TGG 0.587852 6_1:+608593 Msa0867610:CDS
ATTAACTGGAGAACTAACTT+GGG 0.599704 6_1:-609301 None:intergenic
ATGATCATCTCCATCGTCCA+CGG 0.603158 6_1:-608480 None:intergenic
CTTCAGATCAAGTTCAGTGT+GGG 0.604465 6_1:+610585 Msa0867610:CDS
ATGTGAGGTCGAAAATGCCT+AGG 0.605149 6_1:+608633 Msa0867610:CDS
TTGTTGCTTATTTCACCACA+AGG 0.610333 6_1:-608524 None:intergenic
ATGCTGTTGAGAGGCTTGGA+GGG 0.612873 6_1:+608693 Msa0867610:CDS
GTCAAGAGTGAAAGTAGTAG+CGG 0.626941 6_1:+608389 Msa0867610:CDS
AAGCTTGATGAGTCTGGCCA+AGG 0.633707 6_1:+609811 Msa0867610:CDS
CGATTTAAACGAAGAAGCCG+TGG 0.635364 6_1:+608463 Msa0867610:CDS
AAGACTTAGGAACAAGCACA+AGG 0.643200 6_1:+608105 Msa0867610:five_prime_UTR
TGTTTCAGTAGAGGAGACGA+TGG 0.646955 6_1:-610046 None:intergenic
ATTTGAACATAAATTAATGG+CGG 0.647988 6_1:+608343 Msa0867610:exon
CTCCAGTTAATGAATGTGAG+AGG 0.648001 6_1:+609313 Msa0867610:CDS
GTTTCAGTAGAGGAGACGAT+GGG 0.648534 6_1:-610045 None:intergenic
TGTTGCTTATTTCACCACAA+GGG 0.657001 6_1:-608523 None:intergenic
GTGATAAGTGAAGGCCCTTG+TGG 0.681709 6_1:+608509 Msa0867610:CDS
TCTTCAGATCAAGTTCAGTG+TGG 0.702689 6_1:+610584 Msa0867610:CDS
AATCTTTCTAAAGCACAACA+TGG 0.705910 6_1:+608163 Msa0867610:five_prime_UTR
CATGTGATAGTGATAAGTGA+AGG 0.715293 6_1:+608500 Msa0867610:CDS
GTACGGTTAGGCAATATGTG+AGG 0.731217 6_1:+608618 Msa0867610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTAATGTATTTATTTTG+AGG + chr6_1:609239-609258 Msa0867610:intron 10.0%
!!! ATTTAATGTATTTATTTTGA+GGG + chr6_1:609240-609259 Msa0867610:intron 10.0%
!! AAAAATAAAATAAAATTCCA+TGG + chr6_1:610165-610184 Msa0867610:intron 10.0%
!! TATATAAGAAAAATATGTAA+TGG - chr6_1:610433-610452 None:intergenic 10.0%
!! TACTTGTTTATAAAAAAGTA+CGG - chr6_1:608015-608034 None:intergenic 15.0%
!! TTTATAAACAAGTATCTTAT+AGG + chr6_1:608021-608040 Msa0867610:five_prime_UTR 15.0%
!! GATATGTTAGTTAAATATTT+GGG - chr6_1:608223-608242 None:intergenic 15.0%
!! AGATATGTTAGTTAAATATT+TGG - chr6_1:608224-608243 None:intergenic 15.0%
!! AATATTTAACTAACATATCT+TGG + chr6_1:608224-608243 Msa0867610:five_prime_UTR 15.0%
!! AATATAACTATCGTATTATA+TGG - chr6_1:608252-608271 None:intergenic 15.0%
!! GAAATTTGAACATAAATTAA+TGG + chr6_1:608340-608359 Msa0867610:exon 15.0%
!! ACATAATTTAGTGTAATTAT+AGG - chr6_1:609526-609545 None:intergenic 15.0%
!!! CGTTTTAATTATGATTTTTT+TGG + chr6_1:609765-609784 Msa0867610:intron 15.0%
!!! TTTAATTATGATTTTTTTGG+AGG + chr6_1:609768-609787 Msa0867610:intron 15.0%
!!! AAATAGAAACATTTAAAACA+TGG + chr6_1:610478-610497 Msa0867610:intron 15.0%
!!! ACTTGTTTATAAAAAAGTAC+GGG - chr6_1:608014-608033 None:intergenic 20.0%
!! TTCAAATTGTAAGTTTACAT+AGG + chr6_1:608071-608090 Msa0867610:five_prime_UTR 20.0%
!! ATTTGAACATAAATTAATGG+CGG + chr6_1:608343-608362 Msa0867610:exon 20.0%
!! AATAATAATTCTAGTGAAGA+AGG + chr6_1:608581-608600 Msa0867610:CDS 20.0%
!! CATAAATTTATCATTACCTT+TGG + chr6_1:608752-608771 Msa0867610:intron 20.0%
!! CACTTAATCTTATATAGATA+TGG + chr6_1:609200-609219 Msa0867610:intron 20.0%
!! TAACAATATTGGATATATGT+AGG + chr6_1:609271-609290 Msa0867610:intron 20.0%
!! GGTAATATATATTCTTGTAT+AGG + chr6_1:609369-609388 Msa0867610:intron 20.0%
!!! TGATTTTCTCACATTATTAA+CGG + chr6_1:609545-609564 Msa0867610:intron 20.0%
!! TAGATTAATTGTGTTTGTAA+TGG + chr6_1:609619-609638 Msa0867610:intron 20.0%
!! AGATTAATTGTGTTTGTAAT+GGG + chr6_1:609620-609639 Msa0867610:intron 20.0%
!!! TGATTTTGCTAACAATATTA+AGG - chr6_1:609849-609868 None:intergenic 20.0%
!! ACATTAAATGATTGAATGTT+GGG - chr6_1:610285-610304 None:intergenic 20.0%
!! AGTAATAGTGCAAAATATTT+GGG - chr6_1:610387-610406 None:intergenic 20.0%
!! TAGTAATAGTGCAAAATATT+TGG - chr6_1:610388-610407 None:intergenic 20.0%
!! ATAATTGATAGAACTAGAAA+AGG - chr6_1:610764-610783 None:intergenic 20.0%
!! GCACAAGGTTTAATAAATTT+TGG + chr6_1:608120-608139 Msa0867610:five_prime_UTR 25.0%
! GAAGAATTACAAACTTATGT+TGG + chr6_1:608272-608291 Msa0867610:five_prime_UTR 25.0%
! ACAAAAGATAAATGAAGATC+AGG - chr6_1:608671-608690 None:intergenic 25.0%
! TTATGAGTTCTGTATTTGTT+GGG + chr6_1:608791-608810 Msa0867610:intron 25.0%
! ATTATGAATGTACTAAACGT+TGG + chr6_1:608843-608862 Msa0867610:intron 25.0%
! ATTCAGAATCGTAATACATT+AGG - chr6_1:608898-608917 None:intergenic 25.0%
! TAATGTATTACGATTCTGAA+TGG + chr6_1:608897-608916 Msa0867610:intron 25.0%
! AATTCAGAGATCAATATTGT+CGG - chr6_1:608994-609013 None:intergenic 25.0%
! GATCTCTGAATTATATCTTT+AGG + chr6_1:609002-609021 Msa0867610:intron 25.0%
!!! TTAATTTGTGTGTTTTCTAC+CGG + chr6_1:609049-609068 Msa0867610:intron 25.0%
!! TCCATATTATAGTTTGAGTT+GGG - chr6_1:609120-609139 None:intergenic 25.0%
! ATCCATATTATAGTTTGAGT+TGG - chr6_1:609121-609140 None:intergenic 25.0%
!! AAAATGAGAAACAGTTGATA+GGG - chr6_1:609144-609163 None:intergenic 25.0%
! AAAAATGAGAAACAGTTGAT+AGG - chr6_1:609145-609164 None:intergenic 25.0%
!!! GGGTTTTTAGTTAACAATAT+TGG + chr6_1:609260-609279 Msa0867610:intron 25.0%
! TTGATTTATGAACAACACTT+AGG + chr6_1:609397-609416 Msa0867610:intron 25.0%
! ATATTAAATCATGCATGTGA+TGG - chr6_1:609917-609936 None:intergenic 25.0%
!!! TTGCATTGCTCTATTTTTTT+GGG - chr6_1:609966-609985 None:intergenic 25.0%
!! GCTTTGATTTCATTATATGA+TGG - chr6_1:610073-610092 None:intergenic 25.0%
! GACATTAAATGATTGAATGT+TGG - chr6_1:610286-610305 None:intergenic 25.0%
! TTCACATCTATTTGATGTAA+AGG + chr6_1:610618-610637 Msa0867610:CDS 25.0%
! GAAATAATATTGCACCTATT+AGG + chr6_1:610653-610672 Msa0867610:CDS 25.0%
!!! TTGAGATAAGCTTTTTGATT+CGG - chr6_1:610704-610723 None:intergenic 25.0%
! AATCAAAAAGCTTATCTCAA+GGG + chr6_1:610704-610723 Msa0867610:CDS 25.0%
ATTGTAAGTTTACATAGGTG+TGG + chr6_1:608076-608095 Msa0867610:five_prime_UTR 30.0%
!! AATCTTTCTAAAGCACAACA+TGG + chr6_1:608163-608182 Msa0867610:five_prime_UTR 30.0%
!!! GCTCAATTTTTTTGTCTACT+AGG + chr6_1:608294-608313 Msa0867610:five_prime_UTR 30.0%
AATGAGATATCATCACAAGA+AGG + chr6_1:608545-608564 Msa0867610:CDS 30.0%
ATGAGATATCATCACAAGAA+GGG + chr6_1:608546-608565 Msa0867610:CDS 30.0%
AATTCTAGTGAAGAAGGAAA+AGG + chr6_1:608587-608606 Msa0867610:CDS 30.0%
ATTCTAGTGAAGAAGGAAAA+GGG + chr6_1:608588-608607 Msa0867610:CDS 30.0%
! GAACTCATAAGTTGTTTCAT+TGG - chr6_1:608781-608800 None:intergenic 30.0%
CTTATGAGTTCTGTATTTGT+TGG + chr6_1:608790-608809 Msa0867610:intron 30.0%
ATTCAGAGATCAATATTGTC+GGG - chr6_1:608993-609012 None:intergenic 30.0%
!! CTTTAGGAAGTAGTTCTTTT+TGG + chr6_1:609018-609037 Msa0867610:intron 30.0%
TCCCAACTCAAACTATAATA+TGG + chr6_1:609116-609135 Msa0867610:intron 30.0%
ATTAACTGGAGAACTAACTT+GGG - chr6_1:609304-609323 None:intergenic 30.0%
TTTAGTGTAATTATAGGCGT+CGG - chr6_1:609520-609539 None:intergenic 30.0%
AAGAAAAAGACATTCCCAAA+GGG - chr6_1:609723-609742 None:intergenic 30.0%
AAAGAAAAAGACATTCCCAA+AGG - chr6_1:609724-609743 None:intergenic 30.0%
!! TAAGGTTCTGAAATATACCT+TGG - chr6_1:609831-609850 None:intergenic 30.0%
!!! CTTGCATTGCTCTATTTTTT+TGG - chr6_1:609967-609986 None:intergenic 30.0%
CAAAAAAATAGAGCAATGCA+AGG + chr6_1:609965-609984 Msa0867610:CDS 30.0%
CATCACATTATGGATATGTA+TGG + chr6_1:609992-610011 Msa0867610:CDS 30.0%
GATATCATACCCAAACTTTA+GGG - chr6_1:610263-610282 None:intergenic 30.0%
!! TAGTACCCTAAAGTTTTGTA+GGG - chr6_1:610356-610375 None:intergenic 30.0%
!! TTAGTACCCTAAAGTTTTGT+AGG - chr6_1:610357-610376 None:intergenic 30.0%
! TTTTGTAGATTTCATGAGAG+TGG + chr6_1:610538-610557 Msa0867610:intron 30.0%
TTTGTAGATTTCATGAGAGT+GGG + chr6_1:610539-610558 Msa0867610:intron 30.0%
!! TGAGATAAGCTTTTTGATTC+GGG - chr6_1:610703-610722 None:intergenic 30.0%
GAATCAAAAAGCTTATCTCA+AGG + chr6_1:610703-610722 Msa0867610:CDS 30.0%
ATCAAAAAGCTTATCTCAAG+GGG + chr6_1:610705-610724 Msa0867610:CDS 30.0%
ATTGTGTATTCAGTGAAGAT+AGG - chr6_1:610805-610824 None:intergenic 30.0%
TTCACTGAATACACAATGTT+TGG + chr6_1:610808-610827 Msa0867610:CDS 30.0%
! TTTTCTTCGAATCGGTAAAG+AGG - chr6_1:608432-608451 None:intergenic 35.0%
! AACGAACGTTTTCTTCGAAT+CGG - chr6_1:608440-608459 None:intergenic 35.0%
! CATGTGATAGTGATAAGTGA+AGG + chr6_1:608500-608519 Msa0867610:CDS 35.0%
! TGTTGCTTATTTCACCACAA+GGG - chr6_1:608526-608545 None:intergenic 35.0%
! TTGTTGCTTATTTCACCACA+AGG - chr6_1:608527-608546 None:intergenic 35.0%
! GATGTAGTATTTTCCTCTCT+TGG + chr6_1:608921-608940 Msa0867610:intron 35.0%
TTCAGAGATCAATATTGTCG+GGG - chr6_1:608992-609011 None:intergenic 35.0%
!!! AGTAGTTCTTTTTGGTGTCA+TGG + chr6_1:609026-609045 Msa0867610:intron 35.0%
CATTAACTGGAGAACTAACT+TGG - chr6_1:609305-609324 None:intergenic 35.0%
TCTCACATTATTAACGGTGT+CGG + chr6_1:609551-609570 Msa0867610:intron 35.0%
! ACTCATCAAGCTTTTTACTC+CGG - chr6_1:609804-609823 None:intergenic 35.0%
AGTAAAAAGCTTGATGAGTC+TGG + chr6_1:609805-609824 Msa0867610:CDS 35.0%
AAAATAGAGCAATGCAAGGA+AGG + chr6_1:609969-609988 Msa0867610:CDS 35.0%
ATGGATATGTATGGACACTT+TGG + chr6_1:610001-610020 Msa0867610:CDS 35.0%
! TATGATGGTTGTTTCAGTAG+AGG - chr6_1:610058-610077 None:intergenic 35.0%
GGATATCATACCCAAACTTT+AGG - chr6_1:610264-610283 None:intergenic 35.0%
ACCATACCCTACAAAACTTT+AGG + chr6_1:610347-610366 Msa0867610:intron 35.0%
CCATACCCTACAAAACTTTA+GGG + chr6_1:610348-610367 Msa0867610:intron 35.0%
TTATCTCAAGGGGATGATAA+TGG + chr6_1:610715-610734 Msa0867610:CDS 35.0%
! ATGGTGCAAAACATTTTCCA+TGG + chr6_1:610734-610753 Msa0867610:CDS 35.0%
!! ATACACAATGTTTGGTTCTG+AGG + chr6_1:610816-610835 Msa0867610:CDS 35.0%
TTGTGCTTGTTCCTAAGTCT+TGG - chr6_1:608106-608125 None:intergenic 40.0%
AAGACTTAGGAACAAGCACA+AGG + chr6_1:608105-608124 Msa0867610:five_prime_UTR 40.0%
TCACTCTTGACATCTTTGCT+AGG - chr6_1:608380-608399 None:intergenic 40.0%
GTCAAGAGTGAAAGTAGTAG+CGG + chr6_1:608389-608408 Msa0867610:CDS 40.0%
AGTGAAAGTAGTAGCGGTAT+CGG + chr6_1:608395-608414 Msa0867610:CDS 40.0%
! GTATTTTCCTCTCTTGGTCT+TGG + chr6_1:608927-608946 Msa0867610:intron 40.0%
AATATTGTCGGGGAGTTCAA+TGG - chr6_1:608982-609001 None:intergenic 40.0%
!! GTTTGAGTTGGGACGTTAAA+AGG - chr6_1:609109-609128 None:intergenic 40.0%
CTCCAGTTAATGAATGTGAG+AGG + chr6_1:609313-609332 Msa0867610:CDS 40.0%
GTCCTCTCACATTCATTAAC+TGG - chr6_1:609318-609337 None:intergenic 40.0%
ACATGTGAAGAGTCATTTGC+AGG + chr6_1:609348-609367 Msa0867610:CDS 40.0%
TGAACAACACTTAGGAATCG+AGG + chr6_1:609405-609424 Msa0867610:intron 40.0%
GCACATACACTCCTTTGATT+AGG + chr6_1:609462-609481 Msa0867610:intron 40.0%
CTCACATTATTAACGGTGTC+GGG + chr6_1:609552-609571 Msa0867610:intron 40.0%
! TCACATTATTAACGGTGTCG+GGG + chr6_1:609553-609572 Msa0867610:intron 40.0%
AGTTGAGAAACATGCCCTTT+GGG + chr6_1:609706-609725 Msa0867610:intron 40.0%
!! TTTTGGAGGTTCAGATGTAC+CGG + chr6_1:609782-609801 Msa0867610:intron 40.0%
TGCCTATTCGATGTTTCCAA+GGG + chr6_1:609885-609904 Msa0867610:intron 40.0%
AACCCTTGGAAACATCGAAT+AGG - chr6_1:609890-609909 None:intergenic 40.0%
!! CATGTGATGGATAAAACCCT+TGG - chr6_1:609904-609923 None:intergenic 40.0%
TCAAAGCTCATGACACTTCA+AGG + chr6_1:610086-610105 Msa0867610:CDS 40.0%
TCCATGGACATTAACTGATG+TGG + chr6_1:610181-610200 Msa0867610:intron 40.0%
ACCACATCAGTTAATGTCCA+TGG - chr6_1:610185-610204 None:intergenic 40.0%
TGGACATTAACTGATGTGGT+TGG + chr6_1:610185-610204 Msa0867610:intron 40.0%
ACTTCCGAACCCTAAAGTTT+GGG + chr6_1:610251-610270 Msa0867610:intron 40.0%
!! CATACCCAAACTTTAGGGTT+CGG - chr6_1:610258-610277 None:intergenic 40.0%
!! CCCTAAAGTTTTGTAGGGTA+TGG - chr6_1:610351-610370 None:intergenic 40.0%
TCTTCAGATCAAGTTCAGTG+TGG + chr6_1:610584-610603 Msa0867610:CDS 40.0%
CTTCAGATCAAGTTCAGTGT+GGG + chr6_1:610585-610604 Msa0867610:CDS 40.0%
CTTACTTGGCTAGACCTAAT+AGG - chr6_1:610670-610689 None:intergenic 40.0%
! CGGGTAGTAATTGACTTACT+TGG - chr6_1:610684-610703 None:intergenic 40.0%
GGTGTGGAATTCCAAGACTT+AGG + chr6_1:608092-608111 Msa0867610:five_prime_UTR 45.0%
CGATTTAAACGAAGAAGCCG+TGG + chr6_1:608463-608482 Msa0867610:CDS 45.0%
ATGATCATCTCCATCGTCCA+CGG - chr6_1:608483-608502 None:intergenic 45.0%
GTACGGTTAGGCAATATGTG+AGG + chr6_1:608618-608637 Msa0867610:CDS 45.0%
ATGTGAGGTCGAAAATGCCT+AGG + chr6_1:608633-608652 Msa0867610:CDS 45.0%
! CTTTTGTGCATGCTGTTGAG+AGG + chr6_1:608684-608703 Msa0867610:CDS 45.0%
! GTTGTTTCATTGGTCGCCAA+AGG - chr6_1:608771-608790 None:intergenic 45.0%
CAATGTTCCAAGACCAAGAG+AGG - chr6_1:608937-608956 None:intergenic 45.0%
!! CTCTCTTGGTCTTGGAACAT+TGG + chr6_1:608935-608954 Msa0867610:intron 45.0%
! TCAGGGGGTTGATTAATGTC+CGG - chr6_1:609071-609090 None:intergenic 45.0%
CATTAATCAACCCCCTGAAC+TGG + chr6_1:609073-609092 Msa0867610:intron 45.0%
!! GTAATTATAGGCGTCGGTGT+CGG - chr6_1:609514-609533 None:intergenic 45.0%
GTCAGTGTTTGTGTCGTATC+CGG + chr6_1:609577-609596 Msa0867610:intron 45.0%
TGAAGCATGAGCACAAACAC+CGG - chr6_1:609599-609618 None:intergenic 45.0%
GAGTTGAGAAACATGCCCTT+TGG + chr6_1:609705-609724 Msa0867610:intron 45.0%
GTGCCTATTCGATGTTTCCA+AGG + chr6_1:609884-609903 Msa0867610:intron 45.0%
!! GCAAGGAAGGCATCACATTA+TGG + chr6_1:609982-610001 Msa0867610:CDS 45.0%
GTTTCAGTAGAGGAGACGAT+GGG - chr6_1:610048-610067 None:intergenic 45.0%
TGTTTCAGTAGAGGAGACGA+TGG - chr6_1:610049-610068 None:intergenic 45.0%
CACTTCCGAACCCTAAAGTT+TGG + chr6_1:610250-610269 Msa0867610:intron 45.0%
!! GAACTAGAAAAGGTGCTCCA+TGG - chr6_1:610754-610773 None:intergenic 45.0%
CTTCGAATCGGTAAAGAGGC+AGG - chr6_1:608428-608447 None:intergenic 50.0%
GTGATAAGTGAAGGCCCTTG+TGG + chr6_1:608509-608528 Msa0867610:CDS 50.0%
AGTGAAGAAGGAAAAGGGCG+TGG + chr6_1:608593-608612 Msa0867610:CDS 50.0%
! AGGAAAAGGGCGTGGAAGTA+CGG + chr6_1:608601-608620 Msa0867610:CDS 50.0%
CGAAAATGCCTAGGCTTCGT+TGG + chr6_1:608642-608661 Msa0867610:CDS 50.0%
ATGCTGTTGAGAGGCTTGGA+GGG + chr6_1:608693-608712 Msa0867610:CDS 50.0%
AGTGTCAAGACCAGTTCAGG+GGG - chr6_1:609086-609105 None:intergenic 50.0%
GAGTGTCAAGACCAGTTCAG+GGG - chr6_1:609087-609106 None:intergenic 50.0%
! GGAGTGTCAAGACCAGTTCA+GGG - chr6_1:609088-609107 None:intergenic 50.0%
!! AGGAGTGTCAAGACCAGTTC+AGG - chr6_1:609089-609108 None:intergenic 50.0%
ACTGGGACACGCCTAATCAA+AGG - chr6_1:609476-609495 None:intergenic 50.0%
AAGCTTGATGAGTCTGGCCA+AGG + chr6_1:609811-609830 Msa0867610:CDS 50.0%
AACGAAGAAGCCGTGGACGA+TGG + chr6_1:608470-608489 Msa0867610:CDS 55.0%
! AAGGGCGTGGAAGTACGGTT+AGG + chr6_1:608606-608625 Msa0867610:CDS 55.0%
TCAGGAGTCCAACGAAGCCT+AGG - chr6_1:608653-608672 None:intergenic 55.0%
GTGCATGCTGTTGAGAGGCT+TGG + chr6_1:608689-608708 Msa0867610:CDS 55.0%
CATGCTGTTGAGAGGCTTGG+AGG + chr6_1:608692-608711 Msa0867610:CDS 55.0%
GATTAGGCGTGTCCCAGTGT+TGG + chr6_1:609478-609497 Msa0867610:intron 55.0%
GGACACACGTGTCCAACACT+GGG - chr6_1:609493-609512 None:intergenic 55.0%
CGGACACACGTGTCCAACAC+TGG - chr6_1:609494-609513 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 607965 610849 607965 ID=Msa0867610;Name=Msa0867610
chr6_1 mRNA 607965 610849 607965 ID=Msa0867610-mRNA-1;Parent=Msa0867610;Name=Msa0867610-mRNA-1;_AED=0.07;_eAED=0.09;_QI=394|1|0.8|1|1|1|5|0|310
chr6_1 exon 607965 608722 607965 ID=Msa0867610-mRNA-1:exon:187;Parent=Msa0867610-mRNA-1
chr6_1 exon 609293 609369 609293 ID=Msa0867610-mRNA-1:exon:188;Parent=Msa0867610-mRNA-1
chr6_1 exon 609796 609832 609796 ID=Msa0867610-mRNA-1:exon:189;Parent=Msa0867610-mRNA-1
chr6_1 exon 609957 610107 609957 ID=Msa0867610-mRNA-1:exon:190;Parent=Msa0867610-mRNA-1
chr6_1 exon 610546 610849 610546 ID=Msa0867610-mRNA-1:exon:191;Parent=Msa0867610-mRNA-1
chr6_1 five_prime_UTR 607965 608358 607965 ID=Msa0867610-mRNA-1:five_prime_utr;Parent=Msa0867610-mRNA-1
chr6_1 CDS 608359 608722 608359 ID=Msa0867610-mRNA-1:cds;Parent=Msa0867610-mRNA-1
chr6_1 CDS 609293 609369 609293 ID=Msa0867610-mRNA-1:cds;Parent=Msa0867610-mRNA-1
chr6_1 CDS 609796 609832 609796 ID=Msa0867610-mRNA-1:cds;Parent=Msa0867610-mRNA-1
chr6_1 CDS 609957 610107 609957 ID=Msa0867610-mRNA-1:cds;Parent=Msa0867610-mRNA-1
chr6_1 CDS 610546 610849 610546 ID=Msa0867610-mRNA-1:cds;Parent=Msa0867610-mRNA-1
Gene Sequence

>Msa0867610

ATGGCGGAGATTCACATACCTAGCAAAGATGTCAAGAGTGAAAGTAGTAGCGGTATCGGAGCAAAACCTGCCTCTTTACCGATTCGAAGAAAACGTTCGTTCTTCGATTTAAACGAAGAAGCCGTGGACGATGGAGATGATCATGTGATAGTGATAAGTGAAGGCCCTTGTGGTGAAATAAGCAACAATGAGATATCATCACAAGAAGGGAATTTGAGTAGCAATAATAATTCTAGTGAAGAAGGAAAAGGGCGTGGAAGTACGGTTAGGCAATATGTGAGGTCGAAAATGCCTAGGCTTCGTTGGACTCCTGATCTTCATTTATCTTTTGTGCATGCTGTTGAGAGGCTTGGAGGGCAAGAAAGAGCAACACCCAAGTTAGTTCTCCAGTTAATGAATGTGAGAGGACTTAGCATTGCACATGTGAAGAGTCATTTGCAGATGTACCGGAGTAAAAAGCTTGATGAGTCTGGCCAAGTTTTGTCCCAAAAAAATAGAGCAATGCAAGGAAGGCATCACATTATGGATATGTATGGACACTTTGGTGTTGAAAGCAACTATTTGCACCCATCGTCTCCTCTACTGAAACAACCATCATATAATGAAATCAAAGCTCATGACACTTCAAGATTTCATGAGAGTGGGCTTTTCAATAATAATCACGTGATCTTCAGATCAAGTTCAGTGTGGGACAAAGATTTTTCACATCTATTTGATGTAAAGGATGCAATTACAAGAAATAATATTGCACCTATTAGGTCTAGCCAAGTAAGTCAATTACTACCCGAATCAAAAAGCTTATCTCAAGGGGATGATAATGGTGCAAAACATTTTCCATGGAGCACCTTTTCTAGTTCTATCAATTATCAACACAGCTCTTGCAGTCCTATCTTCACTGAATACACAATGTTTGGTTCTGAGGTATGTCCTTAA
Protein sequence

>Msa0867610

MAEIHIPSKDVKSESSSGIGAKPASLPIRRKRSFFDLNEEAVDDGDDHVIVISEGPCGEISNNEISSQEGNLSSNNNSSEEGKGRGSTVRQYVRSKMPRLRWTPDLHLSFVHAVERLGGQERATPKLVLQLMNVRGLSIAHVKSHLQMYRSKKLDESGQVLSQKNRAMQGRHHIMDMYGHFGVESNYLHPSSPLLKQPSYNEIKAHDTSRFHESGLFNNNHVIFRSSSVWDKDFSHLFDVKDAITRNNIAPIRSSQVSQLLPESKSLSQGDDNGAKHFPWSTFSSSINYQHSSCSPIFTEYTMFGSEVCP*