AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0889850


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0889850 MtrunA17_Chr3g0117091 67.164 67 7 1 1 52 1 67 8.99e-23 88.2
Msa0889850 MtrunA17_Chr4g0058631 65.672 67 8 1 1 52 1 67 1.12e-21 85.1
Msa0889850 MtrunA17_Chr7g0270891 62.687 67 10 1 1 52 1 67 1.78e-20 82.0
Msa0889850 MtrunA17_Chr1g0193371 62.687 67 10 1 1 52 1 67 3.41e-20 81.3
Msa0889850 MtrunA17_Chr7g0271241 62.687 67 10 1 1 52 1 67 4.69e-20 80.9
Msa0889850 MtrunA17_Chr1g0211161 63.077 65 9 1 3 52 4 68 1.18e-19 78.2
Msa0889850 MtrunA17_Chr1g0193801 61.194 67 11 1 1 52 1 67 2.62e-19 79.0
Msa0889850 MtrunA17_Chr2g0330041 56.716 67 14 1 1 52 1 67 6.17e-19 77.8
Msa0889850 MtrunA17_Chr8g0342511 50.667 75 22 1 1 60 1 75 1.47e-18 72.0
Msa0889850 MtrunA17_Chr8g0342541 52.055 73 20 1 1 58 1 73 2.17e-18 76.3
Msa0889850 MtrunA17_Chr8g0342521 52.055 73 20 2 1 58 1 73 1.21e-17 73.9
Msa0889850 MtrunA17_Chr8g0342531 53.731 67 16 1 1 52 1 67 2.26e-16 68.2
Msa0889850 MtrunA17_Chr8g0387421 50.725 69 19 1 1 54 1 69 3.89e-16 69.7
Msa0889850 MtrunA17_Chr8g0342461 48.649 74 22 1 1 58 1 74 4.65e-16 66.6
Msa0889850 MtrunA17_Chr8g0342471 48.649 74 22 1 1 58 1 74 4.80e-16 66.6
Msa0889850 MtrunA17_Chr8g0342481 50.000 74 21 1 1 58 1 74 5.71e-16 69.7
Msa0889850 MtrunA17_Chr5g0397231 50.725 69 19 1 1 54 1 69 9.01e-16 68.9
Msa0889850 MtrunA17_Chr2g0309911 52.239 67 16 2 1 52 1 66 1.03e-14 65.9
Msa0889850 MtrunA17_Chr8g0385321 52.239 67 17 1 1 52 1 67 1.41e-13 62.8
Msa0889850 MtrunA17_Chr4g0042611 43.836 73 26 1 1 58 1 73 3.29e-13 58.2
Msa0889850 MtrunA17_Chr3g0115821 46.269 67 21 1 1 52 1 67 4.21e-13 61.6
Msa0889850 MtrunA17_Chr3g0131121 49.254 67 19 1 1 52 1 67 4.60e-13 61.2
Msa0889850 MtrunA17_Chr6g0453471 45.205 73 25 1 1 58 1 73 7.68e-13 60.8
Msa0889850 MtrunA17_Chr2g0328771 60.345 58 4 2 12 52 20 75 1.33e-12 60.1
Msa0889850 MtrunA17_Chr4g0032401 57.143 56 9 1 12 52 17 72 1.48e-12 60.1
Msa0889850 MtrunA17_Chr1g0203411 44.776 67 22 1 1 52 1 67 1.51e-12 59.7
Msa0889850 MtrunA17_Chr4g0039971 46.269 67 21 1 1 52 2 68 3.07e-12 58.9
Msa0889850 MtrunA17_Chr3g0110351 49.254 67 19 1 1 52 1 67 3.62e-12 58.9
Msa0889850 MtrunA17_Chr7g0228891 52.239 67 17 2 1 52 1 67 4.02e-12 58.5
Msa0889850 MtrunA17_Chr5g0434571 49.254 67 19 1 1 52 37 103 7.25e-12 58.2
Msa0889850 MtrunA17_Chr4g0042671 43.836 73 26 1 1 58 1 73 1.16e-11 57.4
Msa0889850 MtrunA17_Chr7g0217741 43.836 73 26 1 1 58 1 73 1.21e-11 57.4
Msa0889850 MtrunA17_Chr6g0470971 56.364 55 9 1 13 52 15 69 1.39e-11 57.0
Msa0889850 MtrunA17_Chr7g0274581 42.029 69 25 1 1 54 1 69 1.42e-11 56.2
Msa0889850 MtrunA17_Chr1g0203441 54.386 57 11 1 13 54 14 70 1.47e-11 57.0
Msa0889850 MtrunA17_Chr2g0311221 45.455 66 21 1 2 52 4 69 1.62e-11 57.0
Msa0889850 MtrunA17_Chr1g0152011 45.588 68 21 1 1 52 1 68 1.68e-11 57.0
Msa0889850 MtrunA17_Chr3g0103281 45.455 66 21 1 1 51 1 66 1.81e-11 57.0
Msa0889850 MtrunA17_Chr7g0261281 44.776 67 22 1 1 52 1 67 2.02e-11 56.6
Msa0889850 MtrunA17_Chr3g0088461 54.237 59 12 1 9 52 19 77 2.07e-11 56.6
Msa0889850 MtrunA17_Chr3g0135311 47.059 68 20 2 1 52 1 68 2.16e-11 56.6
Msa0889850 MtrunA17_Chr5g0410961 43.478 69 24 1 1 54 1 69 2.43e-11 56.6
Msa0889850 MtrunA17_Chr2g0323411 43.836 73 26 1 1 58 1 73 2.54e-11 56.6
Msa0889850 MtrunA17_Chr4g0037141 47.761 67 20 2 1 52 1 67 2.61e-11 56.2
Msa0889850 MtrunA17_Chr1g0151941 43.284 67 22 2 1 51 1 67 2.90e-11 55.8
Msa0889850 MtrunA17_Chr6g0452271 43.662 71 19 2 1 53 1 68 2.93e-11 56.2
Msa0889850 MtrunA17_Chr2g0281071 48.571 70 20 3 1 54 1 70 3.82e-11 55.8
Msa0889850 MtrunA17_Chr7g0264641 47.059 68 20 2 1 52 1 68 5.06e-11 55.5
Msa0889850 MtrunA17_Chr5g0400821 44.776 67 21 1 1 51 1 67 6.70e-11 55.1
Msa0889850 MtrunA17_Chr4g0057031 46.269 67 21 1 1 52 1 67 6.91e-11 55.1
Msa0889850 MtrunA17_Chr2g0323351 46.269 67 21 1 1 52 1 67 9.59e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0889850 AT5G26660.1 62.687 67 10 1 1 52 1 67 3.07e-20 81.6
Msa0889850 AT4G01680.3 61.194 67 11 1 1 52 1 67 1.49e-19 78.2
Msa0889850 AT1G09540.1 61.194 67 11 1 1 52 1 67 2.60e-19 79.3
Msa0889850 AT4G01680.1 61.194 67 11 1 1 52 1 67 3.15e-19 78.6
Msa0889850 AT1G57560.1 61.194 67 11 1 1 52 1 67 7.62e-19 77.4
Msa0889850 AT1G57560.2 61.194 67 11 1 1 52 1 67 9.29e-19 76.6
Msa0889850 AT4G01680.2 51.899 79 11 1 1 52 1 79 1.84e-17 73.9
Msa0889850 AT3G13890.2 55.224 67 15 1 1 52 1 67 3.17e-17 73.6
Msa0889850 AT3G13890.1 48.684 76 15 1 1 52 1 76 6.36e-16 69.7
Msa0889850 AT1G63910.1 50.725 69 19 1 1 54 1 69 1.42e-15 68.9
Msa0889850 AT3G12720.1 52.239 67 16 2 1 52 12 77 6.25e-14 63.9
Msa0889850 AT3G02940.2 47.761 67 20 1 1 52 1 67 1.84e-13 62.8
Msa0889850 AT3G02940.1 47.761 67 20 1 1 52 1 67 2.16e-13 62.8
Msa0889850 AT3G02940.3 47.761 67 20 1 1 52 1 67 2.16e-13 62.8
Msa0889850 AT4G12350.1 47.761 67 20 1 1 52 1 67 2.97e-13 62.0
Msa0889850 AT4G28110.1 45.205 73 25 2 1 58 1 73 4.18e-13 61.6
Msa0889850 AT1G34670.1 46.269 67 21 1 1 52 1 67 4.77e-13 61.6
Msa0889850 AT3G01140.2 45.205 73 25 1 1 58 1 73 5.25e-13 61.6
Msa0889850 AT2G31180.1 46.269 67 21 1 1 52 1 67 5.57e-13 60.8
Msa0889850 AT5G15310.1 46.377 69 22 1 1 54 1 69 1.19e-12 60.5
Msa0889850 AT5G15310.2 46.377 69 22 1 1 54 1 69 1.41e-12 60.5
Msa0889850 AT5G16600.1 50.746 67 18 1 1 52 1 67 1.42e-12 60.5
Msa0889850 AT4G05100.1 50.000 70 19 3 1 54 1 70 1.62e-12 60.1
Msa0889850 AT1G06180.1 49.254 67 19 1 1 52 1 67 1.74e-12 59.7
Msa0889850 AT5G15310.4 46.377 69 22 1 1 54 1 69 1.88e-12 59.7
Msa0889850 AT1G35515.1 46.269 67 21 1 1 52 1 67 2.11e-12 58.9
Msa0889850 AT1G79180.1 48.485 66 19 1 2 52 4 69 3.98e-12 58.9
Msa0889850 AT2G16720.1 47.761 67 20 1 1 52 1 67 3.99e-12 58.9
Msa0889850 AT3G61250.1 47.761 67 20 1 1 52 1 67 4.49e-12 58.9
Msa0889850 AT5G16770.4 44.776 67 22 1 1 52 1 67 5.33e-12 58.5
Msa0889850 AT5G16770.3 44.776 67 22 1 1 52 1 67 5.33e-12 58.5
Msa0889850 AT5G60890.1 41.791 67 24 1 1 52 1 67 5.85e-12 58.5
Msa0889850 AT5G56110.1 45.455 66 21 1 1 51 1 66 5.94e-12 58.5
Msa0889850 AT4G21440.1 47.826 69 21 2 1 54 1 69 7.25e-12 58.5
Msa0889850 AT3G28470.1 47.059 68 21 2 1 53 1 68 7.41e-12 58.2
Msa0889850 AT5G16770.1 44.776 67 22 1 1 52 1 67 7.70e-12 58.2
Msa0889850 AT5G16770.2 44.776 67 22 1 1 52 1 67 7.70e-12 58.2
Msa0889850 AT1G66230.1 49.254 67 19 1 1 52 1 67 9.65e-12 57.8
Msa0889850 AT5G52260.1 54.237 59 12 2 9 52 9 67 1.06e-11 57.8
Msa0889850 AT4G22680.1 47.761 67 20 1 1 52 1 67 1.10e-11 57.8
Msa0889850 AT4G09460.1 46.269 67 21 1 1 52 1 67 1.63e-11 57.0
Msa0889850 AT5G23000.1 44.118 68 22 1 1 52 1 68 1.68e-11 57.4
Msa0889850 AT4G34990.1 47.761 67 20 1 1 52 1 67 1.83e-11 57.0
Msa0889850 AT4G25560.1 55.357 56 10 2 13 53 11 66 2.54e-11 56.6
Msa0889850 AT4G38620.1 47.761 67 20 1 1 52 1 67 2.86e-11 56.6
Msa0889850 AT3G23250.1 43.284 67 23 1 1 52 1 67 4.23e-11 56.2
Msa0889850 AT5G65790.1 44.118 68 22 1 1 52 1 68 4.83e-11 56.2
Msa0889850 AT1G18570.1 42.647 68 23 2 1 52 1 68 5.10e-11 55.8
Msa0889850 AT3G01140.1 44.118 68 23 1 6 58 49 116 5.30e-11 55.8
Msa0889850 AT3G48920.1 52.542 59 13 2 9 52 15 73 5.47e-11 55.8
Msa0889850 AT5G14340.1 47.761 67 20 1 1 52 13 79 6.46e-11 55.5
Msa0889850 AT3G08500.1 55.932 59 11 2 9 52 27 85 6.67e-11 55.8
Msa0889850 AT5G57620.1 44.118 68 22 1 1 52 1 68 9.14e-11 55.5

Find 10 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGCTTCTCATCTTCTTC+AGG 0.263770 6_1:-59995980 None:intergenic
AAATACTAAGAAAATGTTTA+TGG 0.293080 6_1:+59995954 Msa0889850:CDS
GATCTTTCTCTCTTCAAATC+AGG 0.358949 6_1:-59996241 None:intergenic
TCCAACACCAAACATGGTTA+TGG 0.395418 6_1:-59996008 None:intergenic
TTTGCTTTAAACAACAGAAA+TGG 0.418770 6_1:-59995934 None:intergenic
TAAGATGGATAAACTATTTG+AGG 0.452664 6_1:+59996218 Msa0889850:CDS
ATGTATTGCAGGGTTGCAAA+TGG 0.456300 6_1:+59996181 Msa0889850:intron
AAAGCTTCTCATCTTCTTCA+GGG 0.470762 6_1:-59995979 None:intergenic
GAAAGAGTTGTAGATTAAGA+TGG 0.491121 6_1:+59996203 Msa0889850:CDS
AAGCTTCTCATCTTCTTCAG+GGG 0.607980 6_1:-59995978 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATACTAAGAAAATGTTTA+TGG + chr6_1:59995954-59995973 Msa0889850:CDS 15.0%
!! TTGTAAAGTAAAAAAAGATA+AGG - chr6_1:59996120-59996139 None:intergenic 15.0%
!!! TCATTTTTTTATGTATTGCA+GGG + chr6_1:59996171-59996190 Msa0889850:intron 20.0%
!! TTTGCTTTAAACAACAGAAA+TGG - chr6_1:59995937-59995956 None:intergenic 25.0%
!! TTAATATTACCAGCTAGTTT+AGG - chr6_1:59996049-59996068 None:intergenic 25.0%
!!! CTCATTTTTTTATGTATTGC+AGG + chr6_1:59996170-59996189 Msa0889850:intron 25.0%
! TAAGATGGATAAACTATTTG+AGG + chr6_1:59996218-59996237 Msa0889850:CDS 25.0%
TTCAATTCCATAACCATGTT+TGG + chr6_1:59996001-59996020 Msa0889850:intron 30.0%
GAAAGAGTTGTAGATTAAGA+TGG + chr6_1:59996203-59996222 Msa0889850:CDS 30.0%
AAAGCTTCTCATCTTCTTCA+GGG - chr6_1:59995982-59996001 None:intergenic 35.0%
AAAAGCTTCTCATCTTCTTC+AGG - chr6_1:59995983-59996002 None:intergenic 35.0%
GATCTTTCTCTCTTCAAATC+AGG - chr6_1:59996244-59996263 None:intergenic 35.0%
AAGCTTCTCATCTTCTTCAG+GGG - chr6_1:59995981-59996000 None:intergenic 40.0%
!! TCCATAACCATGTTTGGTGT+TGG + chr6_1:59996007-59996026 Msa0889850:intron 40.0%
TCCAACACCAAACATGGTTA+TGG - chr6_1:59996011-59996030 None:intergenic 40.0%
CCAATATCCAACACCAAACA+TGG - chr6_1:59996017-59996036 None:intergenic 40.0%
!! CCATGTTTGGTGTTGGATAT+TGG + chr6_1:59996014-59996033 Msa0889850:intron 40.0%
AGTTGAGTTCCTAAACTAGC+TGG + chr6_1:59996037-59996056 Msa0889850:intron 40.0%
ATGTATTGCAGGGTTGCAAA+TGG + chr6_1:59996181-59996200 Msa0889850:intron 40.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 59995926 59996282 59995926 ID=Msa0889850;Name=Msa0889850
chr6_1 mRNA 59995926 59996282 59995926 ID=Msa0889850-mRNA-1;Parent=Msa0889850;Name=Msa0889850-mRNA-1;_AED=0.32;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|60
chr6_1 exon 59995926 59996017 59995926 ID=Msa0889850-mRNA-1:exon:11351;Parent=Msa0889850-mRNA-1
chr6_1 exon 59996192 59996282 59996192 ID=Msa0889850-mRNA-1:exon:11352;Parent=Msa0889850-mRNA-1
chr6_1 CDS 59995926 59996017 59995926 ID=Msa0889850-mRNA-1:cds;Parent=Msa0889850-mRNA-1
chr6_1 CDS 59996192 59996282 59996192 ID=Msa0889850-mRNA-1:cds;Parent=Msa0889850-mRNA-1
Gene Sequence

>Msa0889850

ATGGGTCGCCATTTCTGTTGTTTAAAGCAAATACTAAGAAAATGTTTATGGTCCCCTGAAGAAGATGAGAAGCTTTTCAATTCCATAACCATGGTTGCAAATGGAAAGAGTTGTAGATTAAGATGGATAAACTATTTGAGGCCTGATTTGAAGAGAGAAAGATCTTATTATCAATCTTCATGA

Protein sequence

>Msa0889850

MGRHFCCLKQILRKCLWSPEEDEKLFNSITMVANGKSCRLRWINYLRPDLKRERSYYQSS*