AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1114030


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1114030 MtrunA17_Chr3g0131571 82.812 64 2 2 26 80 96 159 8.01e-19 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1114030 AT5G51010.1 58.730 63 17 2 26 79 91 153 2.35e-16 68.9

Find 16 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATACATTTCAAACTTAT+CGG 0.341806 7_3:+60640815 None:intergenic
GTGGGAGTTGTAGTTCTTGC+AGG 0.375454 7_3:-60639748 Msa1114030:CDS
ACTCCCACGGCAACTGCAAT+AGG 0.395621 7_3:+60639763 None:intergenic
AATACGTAAGATGTCTGGTT+TGG 0.398048 7_3:-60640882 None:intergenic
ATCAATAGTTTCTTGATCCT+TGG 0.407175 7_3:+60640841 None:intergenic
TTTGTTGACATCTGTTGCAT+AGG 0.431914 7_3:+60639972 None:intergenic
ATTCCAATACGTAAGATGTC+TGG 0.438510 7_3:-60640887 None:intergenic
TAGGGCTCAAACCTTCGTTT+AGG 0.438783 7_3:+60639991 None:intergenic
TTGTTGACATCTGTTGCATA+GGG 0.445845 7_3:+60639973 None:intergenic
TAACTTTATCATATTTGCCA+AGG 0.528266 7_3:-60640858 Msa1114030:CDS
ACTTCCTATTGCAGTTGCCG+TGG 0.541016 7_3:-60639767 Msa1114030:CDS
CTTCCTATTGCAGTTGCCGT+GGG 0.547663 7_3:-60639766 Msa1114030:CDS
ATGTTAGAAAGGCGTTAGAA+AGG 0.548907 7_3:-60639933 Msa1114030:intron
AAATGAAACAGATGTTAGAA+AGG 0.550334 7_3:-60639944 Msa1114030:CDS
TGCAAGAACTACAACTCCCA+CGG 0.635141 7_3:+60639750 None:intergenic
TTTGTGGTGCTCCTAAACGA+AGG 0.648339 7_3:-60640002 Msa1114030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATTATACTGATAAGAA+AGG - chr7_3:60640481-60640500 Msa1114030:intron 15.0%
!! TAATATTATACTGATAAGAA+AGG - chr7_3:60640481-60640500 Msa1114030:intron 15.0%
!! TGGTAAGATATATATTTAGT+TGG - chr7_3:60640140-60640159 Msa1114030:intron 20.0%
!!! ATATATATTTAGTTGGTTTG+TGG - chr7_3:60640147-60640166 Msa1114030:intron 20.0%
!! ATATTAGACCTTAATAAACT+TGG + chr7_3:60640467-60640486 None:intergenic 20.0%
!! ATGTCAAAAACAGAAATAAT+TGG + chr7_3:60640772-60640791 None:intergenic 20.0%
!! TGGTAAGATATATATTTAGT+TGG - chr7_3:60640140-60640159 Msa1114030:intron 20.0%
!!! ATATATATTTAGTTGGTTTG+TGG - chr7_3:60640147-60640166 Msa1114030:intron 20.0%
!! ATATTAGACCTTAATAAACT+TGG + chr7_3:60640467-60640486 None:intergenic 20.0%
!! ATGTCAAAAACAGAAATAAT+TGG + chr7_3:60640772-60640791 None:intergenic 20.0%
! TAACTTTATCATATTTGCCA+AGG - chr7_3:60639731-60639750 Msa1114030:CDS 25.0%
! AGCATACATTTCAAACTTAT+CGG + chr7_3:60639777-60639796 None:intergenic 25.0%
! TTAATGTGTTACAATAATGC+TGG - chr7_3:60639856-60639875 Msa1114030:intron 25.0%
! AAAATGTATTGCCTAAATTG+TGG + chr7_3:60640351-60640370 None:intergenic 25.0%
! TTAGAGTTTCGATAAAATCA+TGG - chr7_3:60640369-60640388 Msa1114030:intron 25.0%
! TTTCTCTACCAAGTTTATTA+AGG - chr7_3:60640456-60640475 Msa1114030:intron 25.0%
! TACTGATAAGAAAGGAAAAA+TGG - chr7_3:60640489-60640508 Msa1114030:intron 25.0%
! ACTGATAAGAAAGGAAAAAT+GGG - chr7_3:60640490-60640509 Msa1114030:intron 25.0%
! TTGTAAGAAGCAAAAGAAAT+AGG - chr7_3:60640518-60640537 Msa1114030:intron 25.0%
! TGTAAGAAGCAAAAGAAATA+GGG - chr7_3:60640519-60640538 Msa1114030:intron 25.0%
! AAATGAAACAGATGTTAGAA+AGG - chr7_3:60640645-60640664 Msa1114030:intron 25.0%
!!! TTTATTATGCTGATTTCAAC+AGG - chr7_3:60640794-60640813 Msa1114030:intron 25.0%
! TAACTTTATCATATTTGCCA+AGG - chr7_3:60639731-60639750 Msa1114030:CDS 25.0%
! AGCATACATTTCAAACTTAT+CGG + chr7_3:60639777-60639796 None:intergenic 25.0%
! TTAATGTGTTACAATAATGC+TGG - chr7_3:60639856-60639875 Msa1114030:intron 25.0%
! AAAATGTATTGCCTAAATTG+TGG + chr7_3:60640351-60640370 None:intergenic 25.0%
! TTAGAGTTTCGATAAAATCA+TGG - chr7_3:60640369-60640388 Msa1114030:intron 25.0%
! TTTCTCTACCAAGTTTATTA+AGG - chr7_3:60640456-60640475 Msa1114030:intron 25.0%
! TACTGATAAGAAAGGAAAAA+TGG - chr7_3:60640489-60640508 Msa1114030:intron 25.0%
! ACTGATAAGAAAGGAAAAAT+GGG - chr7_3:60640490-60640509 Msa1114030:intron 25.0%
! TTGTAAGAAGCAAAAGAAAT+AGG - chr7_3:60640518-60640537 Msa1114030:intron 25.0%
! TGTAAGAAGCAAAAGAAATA+GGG - chr7_3:60640519-60640538 Msa1114030:intron 25.0%
! AAATGAAACAGATGTTAGAA+AGG - chr7_3:60640645-60640664 Msa1114030:intron 25.0%
!!! TTTATTATGCTGATTTCAAC+AGG - chr7_3:60640794-60640813 Msa1114030:intron 25.0%
ATCAATAGTTTCTTGATCCT+TGG + chr7_3:60639751-60639770 None:intergenic 30.0%
CTCAATTTCATCAAGTCATT+AGG + chr7_3:60639904-60639923 None:intergenic 30.0%
! ATTAACTGTCATGGATTACT+TGG - chr7_3:60640238-60640257 Msa1114030:intron 30.0%
! TTAACTGTCATGGATTACTT+GGG - chr7_3:60640239-60640258 Msa1114030:intron 30.0%
TCAAATTCTAAGTGCATGTT+AGG - chr7_3:60640282-60640301 Msa1114030:intron 30.0%
CACAATTGTTACCACAATTT+AGG - chr7_3:60640337-60640356 Msa1114030:intron 30.0%
TCACTACCAATCATAAAGAT+TGG - chr7_3:60640424-60640443 Msa1114030:intron 30.0%
ATGAGACCAATCTTTATGAT+TGG + chr7_3:60640433-60640452 None:intergenic 30.0%
GTAAGAAGCAAAAGAAATAG+GGG - chr7_3:60640520-60640539 Msa1114030:intron 30.0%
GCATTATGATTGTCTGAAAA+TGG - chr7_3:60640554-60640573 Msa1114030:intron 30.0%
ATGCAAAAAGAAAACGACTA+TGG + chr7_3:60640728-60640747 None:intergenic 30.0%
ATCAATAGTTTCTTGATCCT+TGG + chr7_3:60639751-60639770 None:intergenic 30.0%
CTCAATTTCATCAAGTCATT+AGG + chr7_3:60639904-60639923 None:intergenic 30.0%
! ATTAACTGTCATGGATTACT+TGG - chr7_3:60640238-60640257 Msa1114030:intron 30.0%
! TTAACTGTCATGGATTACTT+GGG - chr7_3:60640239-60640258 Msa1114030:intron 30.0%
TCAAATTCTAAGTGCATGTT+AGG - chr7_3:60640282-60640301 Msa1114030:intron 30.0%
CACAATTGTTACCACAATTT+AGG - chr7_3:60640337-60640356 Msa1114030:intron 30.0%
TCACTACCAATCATAAAGAT+TGG - chr7_3:60640424-60640443 Msa1114030:intron 30.0%
ATGAGACCAATCTTTATGAT+TGG + chr7_3:60640433-60640452 None:intergenic 30.0%
GTAAGAAGCAAAAGAAATAG+GGG - chr7_3:60640520-60640539 Msa1114030:intron 30.0%
GCATTATGATTGTCTGAAAA+TGG - chr7_3:60640554-60640573 Msa1114030:intron 30.0%
ATGCAAAAAGAAAACGACTA+TGG + chr7_3:60640728-60640747 None:intergenic 30.0%
ATGTGTTACAATAATGCTGG+TGG - chr7_3:60639859-60639878 Msa1114030:intron 35.0%
TGTGTTACAATAATGCTGGT+GGG - chr7_3:60639860-60639879 Msa1114030:intron 35.0%
GAAATTGAGAACATTTCACG+TGG - chr7_3:60639915-60639934 Msa1114030:intron 35.0%
!!! TCTGTTTCTATGCCGTTTTA+TGG - chr7_3:60639981-60640000 Msa1114030:CDS 35.0%
ATTGTGTGGTGAAACTTTGT+GGG + chr7_3:60640079-60640098 None:intergenic 35.0%
AATTGTGTGGTGAAACTTTG+TGG + chr7_3:60640080-60640099 None:intergenic 35.0%
GTTTCACCACACAATTGTTA+AGG - chr7_3:60640084-60640103 Msa1114030:intron 35.0%
TCAACTCCTTAACAATTGTG+TGG + chr7_3:60640093-60640112 None:intergenic 35.0%
!!! TGATGTGGTTTTTGGCAATT+TGG - chr7_3:60640120-60640139 Msa1114030:intron 35.0%
CGAAACAGAATTAACTGTCA+TGG - chr7_3:60640229-60640248 Msa1114030:intron 35.0%
GTTAGGTTCAGTGAATTTGA+TGG - chr7_3:60640299-60640318 Msa1114030:intron 35.0%
!! CTCTTAAGTTCTGAATTTGG+TGG + chr7_3:60640397-60640416 None:intergenic 35.0%
!! CTGCTCTTAAGTTCTGAATT+TGG + chr7_3:60640400-60640419 None:intergenic 35.0%
! AAATGGTGTTTTGCAGTTTG+TGG - chr7_3:60640571-60640590 Msa1114030:intron 35.0%
TTGTTGACATCTGTTGCATA+GGG + chr7_3:60640619-60640638 None:intergenic 35.0%
TTTGTTGACATCTGTTGCAT+AGG + chr7_3:60640620-60640639 None:intergenic 35.0%
!! ATGTTAGAAAGGCGTTAGAA+AGG - chr7_3:60640656-60640675 Msa1114030:intron 35.0%
ATGTGTTACAATAATGCTGG+TGG - chr7_3:60639859-60639878 Msa1114030:intron 35.0%
TGTGTTACAATAATGCTGGT+GGG - chr7_3:60639860-60639879 Msa1114030:intron 35.0%
GAAATTGAGAACATTTCACG+TGG - chr7_3:60639915-60639934 Msa1114030:intron 35.0%
!!! TCTGTTTCTATGCCGTTTTA+TGG - chr7_3:60639981-60640000 Msa1114030:CDS 35.0%
ATTGTGTGGTGAAACTTTGT+GGG + chr7_3:60640079-60640098 None:intergenic 35.0%
AATTGTGTGGTGAAACTTTG+TGG + chr7_3:60640080-60640099 None:intergenic 35.0%
GTTTCACCACACAATTGTTA+AGG - chr7_3:60640084-60640103 Msa1114030:intron 35.0%
TCAACTCCTTAACAATTGTG+TGG + chr7_3:60640093-60640112 None:intergenic 35.0%
!!! TGATGTGGTTTTTGGCAATT+TGG - chr7_3:60640120-60640139 Msa1114030:intron 35.0%
CGAAACAGAATTAACTGTCA+TGG - chr7_3:60640229-60640248 Msa1114030:intron 35.0%
GTTAGGTTCAGTGAATTTGA+TGG - chr7_3:60640299-60640318 Msa1114030:intron 35.0%
!! CTCTTAAGTTCTGAATTTGG+TGG + chr7_3:60640397-60640416 None:intergenic 35.0%
!! CTGCTCTTAAGTTCTGAATT+TGG + chr7_3:60640400-60640419 None:intergenic 35.0%
! AAATGGTGTTTTGCAGTTTG+TGG - chr7_3:60640571-60640590 Msa1114030:intron 35.0%
TTGTTGACATCTGTTGCATA+GGG + chr7_3:60640619-60640638 None:intergenic 35.0%
TTTGTTGACATCTGTTGCAT+AGG + chr7_3:60640620-60640639 None:intergenic 35.0%
!! ATGTTAGAAAGGCGTTAGAA+AGG - chr7_3:60640656-60640675 Msa1114030:intron 35.0%
GTGTTACAATAATGCTGGTG+GGG - chr7_3:60639861-60639880 Msa1114030:intron 40.0%
GTGCTGCAAAAGCCATAAAA+CGG + chr7_3:60639996-60640015 None:intergenic 40.0%
!! AAGAGAGTAGAAGCACCATT+TGG + chr7_3:60640052-60640071 None:intergenic 40.0%
! ACAACCTCTGATGTGGTTTT+TGG - chr7_3:60640112-60640131 Msa1114030:intron 40.0%
ATTGCCAAAAACCACATCAG+AGG + chr7_3:60640119-60640138 None:intergenic 40.0%
ATGTCTTTCAGAAGCTGTCT+TGG - chr7_3:60640173-60640192 Msa1114030:intron 40.0%
TTTCAGAAGCTGTCTTGGTT+TGG - chr7_3:60640178-60640197 Msa1114030:intron 40.0%
GTTAATTCTGTTTCGTCAGC+AGG + chr7_3:60640224-60640243 None:intergenic 40.0%
TCCAAAGAGATGAAGCAACT+GGG - chr7_3:60640694-60640713 Msa1114030:intron 40.0%
GTGTTACAATAATGCTGGTG+GGG - chr7_3:60639861-60639880 Msa1114030:intron 40.0%
GTGCTGCAAAAGCCATAAAA+CGG + chr7_3:60639996-60640015 None:intergenic 40.0%
!! AAGAGAGTAGAAGCACCATT+TGG + chr7_3:60640052-60640071 None:intergenic 40.0%
! ACAACCTCTGATGTGGTTTT+TGG - chr7_3:60640112-60640131 Msa1114030:intron 40.0%
ATTGCCAAAAACCACATCAG+AGG + chr7_3:60640119-60640138 None:intergenic 40.0%
ATGTCTTTCAGAAGCTGTCT+TGG - chr7_3:60640173-60640192 Msa1114030:intron 40.0%
TTTCAGAAGCTGTCTTGGTT+TGG - chr7_3:60640178-60640197 Msa1114030:intron 40.0%
GTTAATTCTGTTTCGTCAGC+AGG + chr7_3:60640224-60640243 None:intergenic 40.0%
TCCAAAGAGATGAAGCAACT+GGG - chr7_3:60640694-60640713 Msa1114030:intron 40.0%
TTTGTGGTGCTCCTAAACGA+AGG - chr7_3:60640587-60640606 Msa1114030:intron 45.0%
TAGGGCTCAAACCTTCGTTT+AGG + chr7_3:60640601-60640620 None:intergenic 45.0%
CTCCAAAGAGATGAAGCAAC+TGG - chr7_3:60640693-60640712 Msa1114030:intron 45.0%
ACCCAGTTGCTTCATCTCTT+TGG + chr7_3:60640698-60640717 None:intergenic 45.0%
TGCAAGAACTACAACTCCCA+CGG + chr7_3:60640842-60640861 None:intergenic 45.0%
TTTGTGGTGCTCCTAAACGA+AGG - chr7_3:60640587-60640606 Msa1114030:intron 45.0%
TAGGGCTCAAACCTTCGTTT+AGG + chr7_3:60640601-60640620 None:intergenic 45.0%
CTCCAAAGAGATGAAGCAAC+TGG - chr7_3:60640693-60640712 Msa1114030:intron 45.0%
ACCCAGTTGCTTCATCTCTT+TGG + chr7_3:60640698-60640717 None:intergenic 45.0%
TGCAAGAACTACAACTCCCA+CGG + chr7_3:60640842-60640861 None:intergenic 45.0%
TGCTGGTGGGGTTGTAAACA+AGG - chr7_3:60639873-60639892 Msa1114030:intron 50.0%
ATTTGGACTGCTGGAGCAGT+CGG + chr7_3:60640035-60640054 None:intergenic 50.0%
!! AGAAGCACCATTTGGACTGC+TGG + chr7_3:60640044-60640063 None:intergenic 50.0%
GGAGTTGACAACCTCTGATG+TGG - chr7_3:60640105-60640124 Msa1114030:intron 50.0%
ACTTCCTATTGCAGTTGCCG+TGG - chr7_3:60640822-60640841 Msa1114030:CDS 50.0%
CTTCCTATTGCAGTTGCCGT+GGG - chr7_3:60640823-60640842 Msa1114030:CDS 50.0%
GTGGGAGTTGTAGTTCTTGC+AGG - chr7_3:60640841-60640860 Msa1114030:CDS 50.0%
TGCTGGTGGGGTTGTAAACA+AGG - chr7_3:60639873-60639892 Msa1114030:intron 50.0%
ATTTGGACTGCTGGAGCAGT+CGG + chr7_3:60640035-60640054 None:intergenic 50.0%
!! AGAAGCACCATTTGGACTGC+TGG + chr7_3:60640044-60640063 None:intergenic 50.0%
GGAGTTGACAACCTCTGATG+TGG - chr7_3:60640105-60640124 Msa1114030:intron 50.0%
ACTTCCTATTGCAGTTGCCG+TGG - chr7_3:60640822-60640841 Msa1114030:CDS 50.0%
CTTCCTATTGCAGTTGCCGT+GGG - chr7_3:60640823-60640842 Msa1114030:CDS 50.0%
GTGGGAGTTGTAGTTCTTGC+AGG - chr7_3:60640841-60640860 Msa1114030:CDS 50.0%
GACTGCTCCAGCAGTCCAAA+TGG - chr7_3:60640034-60640053 Msa1114030:intron 55.0%
ACTCCCACGGCAACTGCAAT+AGG + chr7_3:60640829-60640848 None:intergenic 55.0%
GACTGCTCCAGCAGTCCAAA+TGG - chr7_3:60640034-60640053 Msa1114030:intron 55.0%
ACTCCCACGGCAACTGCAAT+AGG + chr7_3:60640829-60640848 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 60639717 60640894 60639717 ID=Msa1114030;Name=Msa1114030
chr7_3 mRNA 60639717 60640894 60639717 ID=Msa1114030-mRNA-1;Parent=Msa1114030;Name=Msa1114030-mRNA-1;_AED=0.54;_eAED=0.85;_QI=0|0|0|0.66|0.5|0.66|3|0|80
chr7_3 exon 60640822 60640894 60640822 ID=Msa1114030-mRNA-1:exon:13712;Parent=Msa1114030-mRNA-1
chr7_3 exon 60639934 60640024 60639934 ID=Msa1114030-mRNA-1:exon:13711;Parent=Msa1114030-mRNA-1
chr7_3 exon 60639717 60639795 60639717 ID=Msa1114030-mRNA-1:exon:13710;Parent=Msa1114030-mRNA-1
chr7_3 CDS 60640822 60640894 60640822 ID=Msa1114030-mRNA-1:cds;Parent=Msa1114030-mRNA-1
chr7_3 CDS 60639934 60640024 60639934 ID=Msa1114030-mRNA-1:cds;Parent=Msa1114030-mRNA-1
chr7_3 CDS 60639717 60639795 60639717 ID=Msa1114030-mRNA-1:cds;Parent=Msa1114030-mRNA-1
Gene Sequence

>Msa1114030

ATGTCTGGTTTGGATAACTTTATCATATTTGCCAAGGATCAAGAAACTATTGATGCACCGATAAGTTTGAAATTTTGTGGTGCTCCTAAACGAAGGTTTGAGCCCTATGCAACAGATGTCAACAAAAAAGCAAATGAAACAGATGTTAGAAAGGCGTTAGAAAGGAAAGCACTTCCTATTGCAGTTGCCGTGGGAGTTGTAGTTCTTGCAGGATTATACTTCTATCTCAATAGCACATTTTAG

Protein sequence

>Msa1114030

MSGLDNFIIFAKDQETIDAPISLKFCGAPKRRFEPYATDVNKKANETDVRKALERKALPIAVAVGVVVLAGLYFYLNSTF*