AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0833950


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0833950 MtrunA17_Chr5g0399701 74.419 129 24 3 1 127 1 122 7.64e-33 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCTCATGATTGGGCTTCC+TGG 0.247172 5_4:+7029452 None:intergenic
CTTCCTGGTGGCAGTGATAC+AGG 0.253219 5_4:+7029467 None:intergenic
GTGATACAGGGGAGGGTGCT+TGG 0.269078 5_4:+7029480 None:intergenic
CTCGGGCCCATGCTCATGCT+TGG 0.274606 5_4:+7029383 None:intergenic
CTCATGCTTGGGCTCATGCT+CGG 0.309614 5_4:+7029425 None:intergenic
CTCGGGCTCATGCTCATGAT+TGG 0.309691 5_4:+7029443 None:intergenic
ATGCTACCATTATTCTTTGC+TGG 0.315901 5_4:-7029206 Msa0833950:CDS
CTCATGCTTGGGCTCATACT+TGG 0.360267 5_4:+7029395 None:intergenic
CTGTGAACAACCGCGTTGCT+TGG 0.369585 5_4:+7029242 None:intergenic
TCATGCTTGGGCTCATGCTC+GGG 0.375831 5_4:+7029426 None:intergenic
TTGCTTGGACTTGAAGCAAC+TGG 0.388265 5_4:+7029257 None:intergenic
GAAGGTGATGGTGAGGAAGC+TGG 0.389101 5_4:+7029305 None:intergenic
CTTGGACTCATGCTCATGCT+TGG 0.391770 5_4:+7029413 None:intergenic
TGATACAGGGGAGGGTGCTT+GGG 0.395750 5_4:+7029481 None:intergenic
TCGGGCCCATGCTCATGCTT+GGG 0.410770 5_4:+7029384 None:intergenic
GGGAAAGGTGGGCTCATACT+TGG 0.429231 5_4:+7029347 None:intergenic
TTGGACTCATGCTCATGCTT+GGG 0.445374 5_4:+7029414 None:intergenic
CTTGGACTCATGCTCATGCT+CGG 0.451338 5_4:+7029365 None:intergenic
GCTAGCCATGTCTCTTGCTC+TGG 0.451838 5_4:-7029519 Msa0833950:CDS
GCAGTACCAGCAAAGAATAA+TGG 0.458474 5_4:+7029200 None:intergenic
TTCCTGGTGGCAGTGATACA+GGG 0.459768 5_4:+7029468 None:intergenic
AGGGGAGGGTGCTTGGGCTA+TGG 0.461739 5_4:+7029487 None:intergenic
TCGGGCTCATGCTCATGATT+GGG 0.467264 5_4:+7029444 None:intergenic
TTGGACTCATGCTCATGCTC+GGG 0.527157 5_4:+7029366 None:intergenic
GGAGATGGTGAAGCAGCAGT+AGG 0.535138 5_4:+7029326 None:intergenic
GGTGGCAGTGATACAGGGGA+GGG 0.541381 5_4:+7029473 None:intergenic
ATTATCAGACATCGATTCTC+CGG 0.547569 5_4:+7029280 None:intergenic
GATTCTCCGGTGGAAGGTGA+TGG 0.549766 5_4:+7029293 None:intergenic
TATGAGCCCAAGCATGAGCA+TGG 0.552073 5_4:-7029390 Msa0833950:CDS
TCCTGGTGGCAGTGATACAG+GGG 0.558755 5_4:+7029469 None:intergenic
TGGTGGCAGTGATACAGGGG+AGG 0.573893 5_4:+7029472 None:intergenic
TCCCCTGTATCACTGCCACC+AGG 0.577522 5_4:-7029470 Msa0833950:CDS
GAAGCAGCAGTAGGGAAAGG+TGG 0.580658 5_4:+7029335 None:intergenic
AAGCAGCAGTAGGGAAAGGT+GGG 0.588458 5_4:+7029336 None:intergenic
TCCTCACCATCACCTTCCAC+CGG 0.596602 5_4:-7029299 Msa0833950:CDS
GGTGAAGCAGCAGTAGGGAA+AGG 0.605004 5_4:+7029332 None:intergenic
GATGGTGAGGAAGCTGGAGA+TGG 0.605949 5_4:+7029311 None:intergenic
GAGATGGTGAAGCAGCAGTA+GGG 0.615443 5_4:+7029327 None:intergenic
CTCATGATTGGGCTTCCTGG+TGG 0.617444 5_4:+7029455 None:intergenic
GACATCGATTCTCCGGTGGA+AGG 0.621189 5_4:+7029287 None:intergenic
TCCGGTGGAAGGTGATGGTG+AGG 0.623620 5_4:+7029298 None:intergenic
ATGAGCCCAAGCATGAGCAT+GGG 0.631721 5_4:-7029389 Msa0833950:CDS
TGTCACCAGAGCAAGAGACA+TGG 0.667198 5_4:+7029514 None:intergenic
AGACATGGCTAGCATGAGTG+TGG 0.667612 5_4:+7029529 None:intergenic
ATCAGACATCGATTCTCCGG+TGG 0.683845 5_4:+7029283 None:intergenic
TGCTTCAAGTCCAAGCAACG+CGG 0.698866 5_4:-7029252 Msa0833950:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATCAGACATCGATTCTC+CGG + chr5_4:7029452-7029471 None:intergenic 35.0%
ATGCTACCATTATTCTTTGC+TGG - chr5_4:7029523-7029542 Msa0833950:CDS 35.0%
GCAGTACCAGCAAAGAATAA+TGG + chr5_4:7029532-7029551 None:intergenic 40.0%
TTGGACTCATGCTCATGCTT+GGG + chr5_4:7029318-7029337 None:intergenic 45.0%
TTGCTTGGACTTGAAGCAAC+TGG + chr5_4:7029475-7029494 None:intergenic 45.0%
AGACATGGCTAGCATGAGTG+TGG + chr5_4:7029203-7029222 None:intergenic 50.0%
TGTCACCAGAGCAAGAGACA+TGG + chr5_4:7029218-7029237 None:intergenic 50.0%
TTCCTGGTGGCAGTGATACA+GGG + chr5_4:7029264-7029283 None:intergenic 50.0%
ATGCTCATGATTGGGCTTCC+TGG + chr5_4:7029280-7029299 None:intergenic 50.0%
TCGGGCTCATGCTCATGATT+GGG + chr5_4:7029288-7029307 None:intergenic 50.0%
CTTGGACTCATGCTCATGCT+TGG + chr5_4:7029319-7029338 None:intergenic 50.0%
CTCATGCTTGGGCTCATACT+TGG + chr5_4:7029337-7029356 None:intergenic 50.0%
TATGAGCCCAAGCATGAGCA+TGG - chr5_4:7029339-7029358 Msa0833950:CDS 50.0%
ATGAGCCCAAGCATGAGCAT+GGG - chr5_4:7029310-7029329 Msa0833950:CDS 50.0%
TTGGACTCATGCTCATGCTC+GGG + chr5_4:7029366-7029385 None:intergenic 50.0%
CTTGGACTCATGCTCATGCT+CGG + chr5_4:7029319-7029338 None:intergenic 50.0%
AAGCAGCAGTAGGGAAAGGT+GGG + chr5_4:7029396-7029415 None:intergenic 50.0%
GAGATGGTGAAGCAGCAGTA+GGG + chr5_4:7029405-7029424 None:intergenic 50.0%
!! ATCAGACATCGATTCTCCGG+TGG + chr5_4:7029449-7029468 None:intergenic 50.0%
TGCTTCAAGTCCAAGCAACG+CGG - chr5_4:7029477-7029496 Msa0833950:CDS 50.0%
GCTAGCCATGTCTCTTGCTC+TGG - chr5_4:7029210-7029229 Msa0833950:CDS 55.0%
!! TGATACAGGGGAGGGTGCTT+GGG + chr5_4:7029251-7029270 None:intergenic 55.0%
TCCTGGTGGCAGTGATACAG+GGG + chr5_4:7029263-7029282 None:intergenic 55.0%
CTTCCTGGTGGCAGTGATAC+AGG + chr5_4:7029265-7029284 None:intergenic 55.0%
CTCATGATTGGGCTTCCTGG+TGG + chr5_4:7029277-7029296 None:intergenic 55.0%
CTCGGGCTCATGCTCATGAT+TGG + chr5_4:7029289-7029308 None:intergenic 55.0%
TCATGCTTGGGCTCATGCTC+GGG + chr5_4:7029306-7029325 None:intergenic 55.0%
CTCATGCTTGGGCTCATGCT+CGG + chr5_4:7029307-7029326 None:intergenic 55.0%
! GGGAAAGGTGGGCTCATACT+TGG + chr5_4:7029385-7029404 None:intergenic 55.0%
GAAGCAGCAGTAGGGAAAGG+TGG + chr5_4:7029397-7029416 None:intergenic 55.0%
GGTGAAGCAGCAGTAGGGAA+AGG + chr5_4:7029400-7029419 None:intergenic 55.0%
GGAGATGGTGAAGCAGCAGT+AGG + chr5_4:7029406-7029425 None:intergenic 55.0%
GATGGTGAGGAAGCTGGAGA+TGG + chr5_4:7029421-7029440 None:intergenic 55.0%
! GAAGGTGATGGTGAGGAAGC+TGG + chr5_4:7029427-7029446 None:intergenic 55.0%
TCCTCACCATCACCTTCCAC+CGG - chr5_4:7029430-7029449 Msa0833950:CDS 55.0%
GATTCTCCGGTGGAAGGTGA+TGG + chr5_4:7029439-7029458 None:intergenic 55.0%
!! GACATCGATTCTCCGGTGGA+AGG + chr5_4:7029445-7029464 None:intergenic 55.0%
CTGTGAACAACCGCGTTGCT+TGG + chr5_4:7029490-7029509 None:intergenic 55.0%
!! GTGATACAGGGGAGGGTGCT+TGG + chr5_4:7029252-7029271 None:intergenic 60.0%
GGTGGCAGTGATACAGGGGA+GGG + chr5_4:7029259-7029278 None:intergenic 60.0%
TGGTGGCAGTGATACAGGGG+AGG + chr5_4:7029260-7029279 None:intergenic 60.0%
TCCCCTGTATCACTGCCACC+AGG - chr5_4:7029259-7029278 Msa0833950:CDS 60.0%
TCGGGCCCATGCTCATGCTT+GGG + chr5_4:7029348-7029367 None:intergenic 60.0%
! TCCGGTGGAAGGTGATGGTG+AGG + chr5_4:7029434-7029453 None:intergenic 60.0%
!! AGGGGAGGGTGCTTGGGCTA+TGG + chr5_4:7029245-7029264 None:intergenic 65.0%
CTCGGGCCCATGCTCATGCT+TGG + chr5_4:7029349-7029368 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 7029184 7029567 7029184 ID=Msa0833950;Name=Msa0833950
chr5_4 mRNA 7029184 7029567 7029184 ID=Msa0833950-mRNA-1;Parent=Msa0833950;Name=Msa0833950-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|127
chr5_4 exon 7029184 7029567 7029184 ID=Msa0833950-mRNA-1:exon:3107;Parent=Msa0833950-mRNA-1
chr5_4 CDS 7029184 7029567 7029184 ID=Msa0833950-mRNA-1:cds;Parent=Msa0833950-mRNA-1
Gene Sequence

>Msa0833950

ATGGCACTCACACGTGCCACACTCATGCTAGCCATGTCTCTTGCTCTGGTGACATTGTCCATAGCCCAAGCACCCTCCCCTGTATCACTGCCACCAGGAAGCCCAATCATGAGCATGAGCCCGAGCATGAGCCCAAGCATGAGCATGAGTCCAAGTATGAGCCCAAGCATGAGCATGGGCCCGAGCATGAGCATGAGTCCAAGTATGAGCCCACCTTTCCCTACTGCTGCTTCACCATCTCCAGCTTCCTCACCATCACCTTCCACCGGAGAATCGATGTCTGATAATCCAGTTGCTTCAAGTCCAAGCAACGCGGTTGTTCACAGAAGCAGTGTTTTCATGCTACCATTATTCTTTGCTGGTACTGCACTGCTTCTTGCTTAA

Protein sequence

>Msa0833950

MALTRATLMLAMSLALVTLSIAQAPSPVSLPPGSPIMSMSPSMSPSMSMSPSMSPSMSMGPSMSMSPSMSPPFPTAASPSPASSPSPSTGESMSDNPVASSPSNAVVHRSSVFMLPLFFAGTALLLA*