AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0796580


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0796580 MtrunA17_Chr5g0399701 90.769 65 5 1 17 81 59 122 3.22e-21 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCGGGCCCATGCTCATGCT+TGG 0.274606 5_3:-8635757 None:intergenic
ATGCTACCATTATTCTTTGC+TGG 0.315901 5_3:+8635934 Msa0796580:CDS
CTCATGCTTGGGCTCATACT+TGG 0.360267 5_3:-8635745 None:intergenic
CTGTGAACAACCGCGTTGCT+TGG 0.369585 5_3:-8635898 None:intergenic
TTGCTTGGACTTGAAGCAAC+TGG 0.388265 5_3:-8635883 None:intergenic
GAAGGTGATGGTGAGGAAGC+TGG 0.389101 5_3:-8635835 None:intergenic
TCGGGCCCATGCTCATGCTT+GGG 0.410770 5_3:-8635756 None:intergenic
GGGAAAGGTGGGCTCATACT+TGG 0.429231 5_3:-8635793 None:intergenic
CTTGGACTCATGCTCATGCT+CGG 0.451338 5_3:-8635775 None:intergenic
GCAGTACCAGCAAAGAATAA+TGG 0.458474 5_3:-8635940 None:intergenic
TTGGACTCATGCTCATGCTC+GGG 0.527157 5_3:-8635774 None:intergenic
GGAGATGGTGAAGCAGCAGT+AGG 0.535138 5_3:-8635814 None:intergenic
ATTATCAGACATCGATTCTC+CGG 0.547569 5_3:-8635860 None:intergenic
GATTCTCCGGTGGAAGGTGA+TGG 0.549766 5_3:-8635847 None:intergenic
TATGAGCCCAAGCATGAGCA+TGG 0.552073 5_3:+8635750 Msa0796580:CDS
GAAGCAGCAGTAGGGAAAGG+TGG 0.580658 5_3:-8635805 None:intergenic
AAGCAGCAGTAGGGAAAGGT+GGG 0.588458 5_3:-8635804 None:intergenic
TCCTCACCATCACCTTCCAC+CGG 0.596602 5_3:+8635841 Msa0796580:CDS
GGTGAAGCAGCAGTAGGGAA+AGG 0.605004 5_3:-8635808 None:intergenic
GATGGTGAGGAAGCTGGAGA+TGG 0.605949 5_3:-8635829 None:intergenic
GAGATGGTGAAGCAGCAGTA+GGG 0.615443 5_3:-8635813 None:intergenic
GACATCGATTCTCCGGTGGA+AGG 0.621189 5_3:-8635853 None:intergenic
TCCGGTGGAAGGTGATGGTG+AGG 0.623620 5_3:-8635842 None:intergenic
ATGAGCCCAAGCATGAGCAT+GGG 0.631721 5_3:+8635751 Msa0796580:CDS
ATCAGACATCGATTCTCCGG+TGG 0.683845 5_3:-8635857 None:intergenic
TGCTTCAAGTCCAAGCAACG+CGG 0.698866 5_3:+8635888 Msa0796580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATCAGACATCGATTCTC+CGG - chr5_3:8635863-8635882 None:intergenic 35.0%
ATGCTACCATTATTCTTTGC+TGG + chr5_3:8635934-8635953 Msa0796580:CDS 35.0%
GCAGTACCAGCAAAGAATAA+TGG - chr5_3:8635943-8635962 None:intergenic 40.0%
TTGCTTGGACTTGAAGCAAC+TGG - chr5_3:8635886-8635905 None:intergenic 45.0%
CTCATGCTTGGGCTCATACT+TGG - chr5_3:8635748-8635767 None:intergenic 50.0%
TATGAGCCCAAGCATGAGCA+TGG + chr5_3:8635750-8635769 Msa0796580:CDS 50.0%
ATGAGCCCAAGCATGAGCAT+GGG + chr5_3:8635751-8635770 Msa0796580:CDS 50.0%
TTGGACTCATGCTCATGCTC+GGG - chr5_3:8635777-8635796 None:intergenic 50.0%
CTTGGACTCATGCTCATGCT+CGG - chr5_3:8635778-8635797 None:intergenic 50.0%
AAGCAGCAGTAGGGAAAGGT+GGG - chr5_3:8635807-8635826 None:intergenic 50.0%
GAGATGGTGAAGCAGCAGTA+GGG - chr5_3:8635816-8635835 None:intergenic 50.0%
!! ATCAGACATCGATTCTCCGG+TGG - chr5_3:8635860-8635879 None:intergenic 50.0%
TGCTTCAAGTCCAAGCAACG+CGG + chr5_3:8635888-8635907 Msa0796580:CDS 50.0%
! GGGAAAGGTGGGCTCATACT+TGG - chr5_3:8635796-8635815 None:intergenic 55.0%
GAAGCAGCAGTAGGGAAAGG+TGG - chr5_3:8635808-8635827 None:intergenic 55.0%
GGTGAAGCAGCAGTAGGGAA+AGG - chr5_3:8635811-8635830 None:intergenic 55.0%
GGAGATGGTGAAGCAGCAGT+AGG - chr5_3:8635817-8635836 None:intergenic 55.0%
GATGGTGAGGAAGCTGGAGA+TGG - chr5_3:8635832-8635851 None:intergenic 55.0%
! GAAGGTGATGGTGAGGAAGC+TGG - chr5_3:8635838-8635857 None:intergenic 55.0%
TCCTCACCATCACCTTCCAC+CGG + chr5_3:8635841-8635860 Msa0796580:CDS 55.0%
GATTCTCCGGTGGAAGGTGA+TGG - chr5_3:8635850-8635869 None:intergenic 55.0%
!! GACATCGATTCTCCGGTGGA+AGG - chr5_3:8635856-8635875 None:intergenic 55.0%
CTGTGAACAACCGCGTTGCT+TGG - chr5_3:8635901-8635920 None:intergenic 55.0%
TCGGGCCCATGCTCATGCTT+GGG - chr5_3:8635759-8635778 None:intergenic 60.0%
! TCCGGTGGAAGGTGATGGTG+AGG - chr5_3:8635845-8635864 None:intergenic 60.0%
CTCGGGCCCATGCTCATGCT+TGG - chr5_3:8635760-8635779 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 8635733 8635978 8635733 ID=Msa0796580;Name=Msa0796580
chr5_3 mRNA 8635733 8635978 8635733 ID=Msa0796580-mRNA-1;Parent=Msa0796580;Name=Msa0796580-mRNA-1;_AED=0.19;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|81
chr5_3 exon 8635733 8635978 8635733 ID=Msa0796580-mRNA-1:exon:3642;Parent=Msa0796580-mRNA-1
chr5_3 CDS 8635733 8635978 8635733 ID=Msa0796580-mRNA-1:cds;Parent=Msa0796580-mRNA-1
Gene Sequence

>Msa0796580

ATGAGCATGAGTCCAAGTATGAGCCCAAGCATGAGCATGGGCCCGAGCATGAGCATGAGTCCAAGTATGAGCCCACCTTTCCCTACTGCTGCTTCACCATCTCCAGCTTCCTCACCATCACCTTCCACCGGAGAATCGATGTCTGATAATCCAGTTGCTTCAAGTCCAAGCAACGCGGTTGTTCACAGAAGCAGTGTTTTCATGCTACCATTATTCTTTGCTGGTACTGCACTGCTTCTTGCTTAA

Protein sequence

>Msa0796580

MSMSPSMSPSMSMGPSMSMSPSMSPPFPTAASPSPASSPSPSTGESMSDNPVASSPSNAVVHRSSVFMLPLFFAGTALLLA*