AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0599420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0599420 MtrunA17_Chr4g0023621 97.500 160 4 0 1 160 1 160 1.57e-112 316
Msa0599420 MtrunA17_Chr2g0323081 71.519 158 45 0 1 158 1 158 1.65e-80 235
Msa0599420 MtrunA17_Chr4g0049001 55.696 158 70 0 1 158 1 158 9.91e-68 202
Msa0599420 MtrunA17_Chr2g0280491 55.063 158 71 0 1 158 1 158 2.90e-59 182
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0599420 AT2G32280.1 63.750 160 58 0 1 160 1 160 1.39e-77 228
Msa0599420 AT2G32280.2 63.750 160 58 0 1 160 1 160 1.39e-77 228
Msa0599420 AT4G21310.1 63.291 158 58 0 1 158 1 158 1.81e-72 215
Msa0599420 AT4G21310.2 60.000 120 48 0 39 158 1 120 1.37e-51 161
Msa0599420 AT1G11500.1 39.375 160 90 1 6 158 6 165 1.43e-33 117
Msa0599420 AT1G11500.3 39.375 160 90 1 6 158 11 170 2.17e-33 117
Msa0599420 AT1G11500.2 42.105 114 66 0 45 158 24 137 2.14e-27 100
Msa0599420 AT1G05291.1 40.000 145 80 3 12 153 23 163 6.62e-24 92.8

Find 62 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAAAACACAAATATTTC+TGG 0.152103 4_2:+38973975 None:intergenic
TGCGATTATGGCCCCTGTTT+TGG 0.195648 4_2:-38971554 Msa0599420:three_prime_UTR
TTCTCAACAAGTTGCGATTA+TGG 0.218709 4_2:-38971566 Msa0599420:three_prime_UTR
GATGTTGCAGCTGGTATTCT+TGG 0.255223 4_2:-38973899 Msa0599420:CDS
AATCATATTTCCCTTATGTT+AGG 0.259505 4_2:+38971604 None:intergenic
GTTGGGTGCAGCAGTGCTAT+TGG 0.296588 4_2:-38973657 Msa0599420:CDS
TGATTTACTCATTATTATCT+TGG 0.313721 4_2:-38971734 Msa0599420:three_prime_UTR
TTGGGTGCAGCAGTGCTATT+GGG 0.315421 4_2:-38973656 Msa0599420:CDS
CAATTGTGTTTGTTCTCAAC+AGG 0.330421 4_2:-38973597 Msa0599420:CDS
ATTGCAACCACCTAACAAAT+TGG 0.332978 4_2:+38973616 None:intergenic
GGATTGTCCATGCTGGTAAT+AGG 0.335567 4_2:-38972032 Msa0599420:CDS
AGTCATGATGCCTTCATGTT+GGG 0.345983 4_2:-38973680 Msa0599420:CDS
GGCAAAAGAGACCCATGTTT+AGG 0.350325 4_2:-38971647 Msa0599420:three_prime_UTR
TATATTACCAGGTTAAAATA+AGG 0.351790 4_2:+38973531 None:intergenic
GATTGTCCATGCTGGTAATA+GGG 0.353853 4_2:-38972031 Msa0599420:CDS
GACGCCACGTGTCCCAAAAC+AGG 0.355530 4_2:+38971541 None:intergenic
CAACATCCATGATAACAATT+AGG 0.363009 4_2:+38973915 None:intergenic
GCAAAAGAGACCCATGTTTA+GGG 0.381325 4_2:-38971646 Msa0599420:three_prime_UTR
ATCATATTTCCCTTATGTTA+GGG 0.383201 4_2:+38971605 None:intergenic
CATTGTTACCACTGCTTCCA+TGG 0.385326 4_2:-38971898 Msa0599420:CDS
ATCAGGGTTGTGGTGGCTGT+TGG 0.394905 4_2:-38972053 Msa0599420:intron
CACCATCACTTTCTGTCCAT+TGG 0.397413 4_2:-38971966 Msa0599420:CDS
GATGCCTTCATGTTGGGGTT+GGG 0.408304 4_2:-38973674 Msa0599420:CDS
AAAGTACTGAGAGTTGTCTA+TGG 0.408478 4_2:+38973555 None:intergenic
AGCTGGTATTCTTGGCTTTG+AGG 0.413890 4_2:-38973891 Msa0599420:CDS
TTGTCTCCTAATTGTTATCA+TGG 0.414420 4_2:-38973921 Msa0599420:CDS
TTGAAAACACAAATATTTCT+GGG 0.422188 4_2:+38973976 None:intergenic
TGATGCCTTCATGTTGGGGT+TGG 0.426974 4_2:-38973675 Msa0599420:CDS
CGCAACTTGTTGAGAAGATA+AGG 0.431968 4_2:+38971573 None:intergenic
AAAACAGGTTAAGCATTTGA+AGG 0.491275 4_2:-38973729 Msa0599420:intron
CATCACTTTCTGTCCATTGG+TGG 0.495015 4_2:-38971963 Msa0599420:CDS
TGTGTTGTTGATCAGGGTTG+TGG 0.499842 4_2:-38972063 Msa0599420:intron
TAGGGGCTATGTCAAACAAC+AGG 0.503152 4_2:-38972013 Msa0599420:CDS
TACAAAATGGCTAGAGTAGG+AGG 0.513279 4_2:-38973953 Msa0599420:exon
AACATGAAGGCATCATGACT+TGG 0.534290 4_2:+38973683 None:intergenic
GTCATGATGCCTTCATGTTG+GGG 0.535583 4_2:-38973679 Msa0599420:CDS
GGCTGTTGGATTGTCCATGC+TGG 0.535782 4_2:-38972039 Msa0599420:CDS
TTCTCAACAGGAATTTGAGA+AGG 0.542952 4_2:-38973585 Msa0599420:CDS
CATATTTCCCTTATGTTAGG+GGG 0.545265 4_2:+38971607 None:intergenic
AAAACATGAAATTCCACCAA+TGG 0.549360 4_2:+38971950 None:intergenic
TCTTACAAAATACTAATCCA+TGG 0.550482 4_2:+38971881 None:intergenic
AGAGCATATTTCCCTAAACA+TGG 0.551374 4_2:+38971635 None:intergenic
AGGTTAAGCATTTGAAGGTG+TGG 0.557434 4_2:-38973724 Msa0599420:CDS
CAATACAAAATGGCTAGAGT+AGG 0.563356 4_2:-38973956 Msa0599420:exon
AAGTCATGATGCCTTCATGT+TGG 0.571287 4_2:-38973681 Msa0599420:CDS
ATTGTCCATGCTGGTAATAG+GGG 0.576738 4_2:-38972030 Msa0599420:CDS
GTTATCATGGATGTTGCAGC+TGG 0.584347 4_2:-38973908 Msa0599420:CDS
GTTGTTGATCAGGGTTGTGG+TGG 0.588245 4_2:-38972060 Msa0599420:intron
AAGTACTGAGAGTTGTCTAT+GGG 0.588754 4_2:+38973556 None:intergenic
ATACTAATCCATGGAAGCAG+TGG 0.591196 4_2:+38971890 None:intergenic
TATGTCAAACAACAGGTCAA+AGG 0.592422 4_2:-38972006 Msa0599420:CDS
ACGCCACGTGTCCCAAAACA+GGG 0.593408 4_2:+38971542 None:intergenic
TCATATTTCCCTTATGTTAG+GGG 0.602228 4_2:+38971606 None:intergenic
TGCACCCAACCCCAACATGA+AGG 0.603813 4_2:+38973670 None:intergenic
TACTGAGAGTTGTCTATGGG+AGG 0.604411 4_2:+38973559 None:intergenic
AACAGGTCAAAGGATTCTTG+TGG 0.609881 4_2:-38971996 Msa0599420:CDS
GGCTGAAATAGCACAAAACA+AGG 0.619758 4_2:-38973870 Msa0599420:intron
CATAGCCCCTATTACCAGCA+TGG 0.640867 4_2:+38972025 None:intergenic
AGTTCTAAGATTAGAAACAG+TGG 0.646953 4_2:+38974037 None:intergenic
GAGCATATTTCCCTAAACAT+GGG 0.648214 4_2:+38971636 None:intergenic
CACCAATGGACAGAAAGTGA+TGG 0.662217 4_2:+38971964 None:intergenic
CGCCACGTGTCCCAAAACAG+GGG 0.699563 4_2:+38971543 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGAAATAATTAATAACA+AGG - chr4_2:38971792-38971811 Msa0599420:three_prime_UTR 10.0%
!! TAAAATATATTCCAAAATGT+TGG + chr4_2:38971755-38971774 None:intergenic 15.0%
!!! TTTTTTATTTTCAACAAAAC+AGG - chr4_2:38971840-38971859 Msa0599420:three_prime_UTR 15.0%
!!! CCAAATAAAATATTTTACAT+AGG + chr4_2:38972202-38972221 None:intergenic 15.0%
!!! CCTATGTAAAATATTTTATT+TGG - chr4_2:38972199-38972218 Msa0599420:intron 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG + chr4_2:38972527-38972546 None:intergenic 15.0%
!! AGAAAAAATTAAAGTTGTAT+TGG + chr4_2:38972567-38972586 None:intergenic 15.0%
!! AAATAGAATGAAATAATCAT+TGG + chr4_2:38972693-38972712 None:intergenic 15.0%
!! AAAATGTTTGAATATCTAAT+CGG - chr4_2:38972981-38973000 Msa0599420:intron 15.0%
!!! AAATAGTACCTTTTTTAATT+GGG - chr4_2:38973376-38973395 Msa0599420:intron 15.0%
!! ATATTTAGTGAATGATTATT+TGG + chr4_2:38973484-38973503 None:intergenic 15.0%
!! TTGAAAACACAAATATTTCT+GGG + chr4_2:38971611-38971630 None:intergenic 20.0%
!! ATTGAAAACACAAATATTTC+TGG + chr4_2:38971612-38971631 None:intergenic 20.0%
!!! TTTGTGTTTTCAATACAAAA+TGG - chr4_2:38971618-38971637 Msa0599420:three_prime_UTR 20.0%
!! TATATTACCAGGTTAAAATA+AGG + chr4_2:38972056-38972075 None:intergenic 20.0%
!!! ATATTTTACATAGGAAAAAC+CGG + chr4_2:38972193-38972212 None:intergenic 20.0%
!! CAAATAATCTTCAAATTACA+AGG + chr4_2:38972232-38972251 None:intergenic 20.0%
!! CTTGTAATTTGAAGATTATT+TGG - chr4_2:38972230-38972249 Msa0599420:intron 20.0%
!! AAAATTTGCACAAATTAACA+GGG + chr4_2:38972343-38972362 None:intergenic 20.0%
!!! AATTTGAAGTTTTTTGGTTT+TGG + chr4_2:38972417-38972436 None:intergenic 20.0%
!!! ATCCAAAAAGAAAATTTTAC+AGG - chr4_2:38972448-38972467 Msa0599420:intron 20.0%
!!! TCCAAAAAGAAAATTTTACA+GGG - chr4_2:38972449-38972468 Msa0599420:intron 20.0%
!!! TGTCTTTTTCTTATAAAGTA+AGG - chr4_2:38972644-38972663 Msa0599420:intron 20.0%
!!! GTCTTTTTCTTATAAAGTAA+GGG - chr4_2:38972645-38972664 Msa0599420:intron 20.0%
!! AAATGTTTGAATATCTAATC+GGG - chr4_2:38972982-38973001 Msa0599420:intron 20.0%
!!! ATTTTAAAGAAAAGTGAAAG+TGG - chr4_2:38973069-38973088 Msa0599420:intron 20.0%
!!! ACAATTAGACCATTTTAATT+CGG - chr4_2:38973188-38973207 Msa0599420:intron 20.0%
!!! CAAATAGTACCTTTTTTAAT+TGG - chr4_2:38973375-38973394 Msa0599420:intron 20.0%
!!! AATAGTACCTTTTTTAATTG+GGG - chr4_2:38973377-38973396 Msa0599420:intron 20.0%
!! TATTGGATCAAAATACATTT+AGG + chr4_2:38973424-38973443 None:intergenic 20.0%
!! TATCAGTTACATTTAGTTAT+TGG + chr4_2:38973441-38973460 None:intergenic 20.0%
!! TGATTTACTCATTATTATCT+TGG - chr4_2:38973850-38973869 Msa0599420:intron 20.0%
!!! CTTTTGCCTTTTTTTAATAA+GGG + chr4_2:38973925-38973944 None:intergenic 20.0%
!!! TCTTTTGCCTTTTTTTAATA+AGG + chr4_2:38973926-38973945 None:intergenic 20.0%
! TTGGAATAACAAAACTAGTT+AGG - chr4_2:38972101-38972120 Msa0599420:intron 25.0%
! AAATTTGCACAAATTAACAG+GGG + chr4_2:38972342-38972361 None:intergenic 25.0%
! CAAAATTTGCACAAATTAAC+AGG + chr4_2:38972344-38972363 None:intergenic 25.0%
!!! CTGTTAATTTGTGCAAATTT+TGG - chr4_2:38972342-38972361 Msa0599420:intron 25.0%
! ATTGTACAAAATCGATGATA+TGG + chr4_2:38972378-38972397 None:intergenic 25.0%
!!! CCTTGTAATTTGAAGTTTTT+TGG + chr4_2:38972423-38972442 None:intergenic 25.0%
! CCAAAAAACTTCAAATTACA+AGG - chr4_2:38972420-38972439 Msa0599420:intron 25.0%
! AAAACTTCAAATTACAAGGA+CGG - chr4_2:38972424-38972443 Msa0599420:intron 25.0%
!! CCAAAAAGAAAATTTTACAG+GGG - chr4_2:38972450-38972469 Msa0599420:intron 25.0%
!! CAAAAAGAAAATTTTACAGG+GGG - chr4_2:38972451-38972470 Msa0599420:intron 25.0%
!!! TTTAAAATAACTGTCGCTTT+AGG + chr4_2:38972735-38972754 None:intergenic 25.0%
!!! AAGCGACAGTTATTTTAAAA+CGG - chr4_2:38972736-38972755 Msa0599420:intron 25.0%
! TTGTATCATTGTGATACTTA+TGG + chr4_2:38972824-38972843 None:intergenic 25.0%
! ACGTAAATTTGGAGAAAATT+GGG + chr4_2:38972877-38972896 None:intergenic 25.0%
! AACGTAAATTTGGAGAAAAT+TGG + chr4_2:38972878-38972897 None:intergenic 25.0%
! AATGTTTGAATATCTAATCG+GGG - chr4_2:38972983-38973002 Msa0599420:intron 25.0%
! ATTTCACAATCATGATACTT+AGG - chr4_2:38973156-38973175 Msa0599420:intron 25.0%
! TTTCACAATCATGATACTTA+GGG - chr4_2:38973157-38973176 Msa0599420:intron 25.0%
!!! CAATTAGACCATTTTAATTC+GGG - chr4_2:38973189-38973208 Msa0599420:intron 25.0%
! TATGTTTCCGAATAAAACTA+TGG + chr4_2:38973329-38973348 None:intergenic 25.0%
! TCTTACAAAATACTAATCCA+TGG + chr4_2:38973706-38973725 None:intergenic 25.0%
!! CTTTTGTAATAATTGTGCTA+AGG - chr4_2:38973816-38973835 Msa0599420:intron 25.0%
!!! GCAACATTTTAAAACTTATG+GGG + chr4_2:38973893-38973912 None:intergenic 25.0%
!!! CGCAACATTTTAAAACTTAT+GGG + chr4_2:38973894-38973913 None:intergenic 25.0%
!!! TCGCAACATTTTAAAACTTA+TGG + chr4_2:38973895-38973914 None:intergenic 25.0%
!!! GTTTTAAAATGTTGCGATTT+TGG - chr4_2:38973898-38973917 Msa0599420:CDS 25.0%
! TTTGGTCCCTTATTAAAAAA+AGG - chr4_2:38973916-38973935 Msa0599420:CDS 25.0%
! ATCATATTTCCCTTATGTTA+GGG + chr4_2:38973982-38974001 None:intergenic 25.0%
! AATCATATTTCCCTTATGTT+AGG + chr4_2:38973983-38974002 None:intergenic 25.0%
!! AGTTCTAAGATTAGAAACAG+TGG + chr4_2:38971550-38971569 None:intergenic 30.0%
TTGTCTCCTAATTGTTATCA+TGG - chr4_2:38971663-38971682 Msa0599420:three_prime_UTR 30.0%
CAACATCCATGATAACAATT+AGG + chr4_2:38971672-38971691 None:intergenic 30.0%
! TATGCTTGAATCCAACATTT+TGG - chr4_2:38971741-38971760 Msa0599420:three_prime_UTR 30.0%
AAAACAGGTTAAGCATTTGA+AGG - chr4_2:38971855-38971874 Msa0599420:three_prime_UTR 30.0%
!!! CATGTTTTAGCCAATTTGTT+AGG - chr4_2:38971958-38971977 Msa0599420:CDS 30.0%
!!! TACTTTGCCTTATTTTAACC+TGG - chr4_2:38972046-38972065 Msa0599420:CDS 30.0%
!!! TAGTTTTGTTATTCCAAAGC+AGG + chr4_2:38972098-38972117 None:intergenic 30.0%
ACCGAAATTTCCTGAAATTA+CGG + chr4_2:38972284-38972303 None:intergenic 30.0%
! TGAAATTTGCTCAAATTGCA+GGG + chr4_2:38972313-38972332 None:intergenic 30.0%
! CCCCTGTAAAATTTTCTTTT+TGG + chr4_2:38972453-38972472 None:intergenic 30.0%
!!! GACAGTTATTTTAAAACGGA+GGG - chr4_2:38972740-38972759 Msa0599420:intron 30.0%
AATTTGGAGAAAATTGGGAT+GGG + chr4_2:38972872-38972891 None:intergenic 30.0%
AAATTTGGAGAAAATTGGGA+TGG + chr4_2:38972873-38972892 None:intergenic 30.0%
ATGTCATCTCAACGTAAATT+TGG + chr4_2:38972888-38972907 None:intergenic 30.0%
GAATTAAAGAATAGCCACAT+TGG + chr4_2:38972942-38972961 None:intergenic 30.0%
!!! CTACGTACCATAGTTTTATT+CGG - chr4_2:38973319-38973338 Msa0599420:intron 30.0%
ATGAACTCCCCAATTAAAAA+AGG + chr4_2:38973387-38973406 None:intergenic 30.0%
GATCAACAACACAATCAAAA+GGG + chr4_2:38973514-38973533 None:intergenic 30.0%
TGATCAACAACACAATCAAA+AGG + chr4_2:38973515-38973534 None:intergenic 30.0%
!! TTTTGATTGTGTTGTTGATC+AGG - chr4_2:38973514-38973533 Msa0599420:intron 30.0%
! TTTGATTGTGTTGTTGATCA+GGG - chr4_2:38973515-38973534 Msa0599420:intron 30.0%
AAAACATGAAATTCCACCAA+TGG + chr4_2:38973637-38973656 None:intergenic 30.0%
!! GGAATTTCATGTTTTGTTCA+TGG - chr4_2:38973642-38973661 Msa0599420:CDS 30.0%
!!! GTTCATGTTTTTAGTGCTTT+TGG - chr4_2:38973748-38973767 Msa0599420:intron 30.0%
TCATATTTCCCTTATGTTAG+GGG + chr4_2:38973981-38974000 None:intergenic 30.0%
! GATTTTCTTTCTGTTCTAGC+TGG + chr4_2:38971589-38971608 None:intergenic 35.0%
CAATACAAAATGGCTAGAGT+AGG - chr4_2:38971628-38971647 Msa0599420:three_prime_UTR 35.0%
!!! GTTTTAGCCAATTTGTTAGG+TGG - chr4_2:38971961-38971980 Msa0599420:CDS 35.0%
ATTGCAACCACCTAACAAAT+TGG + chr4_2:38971971-38971990 None:intergenic 35.0%
CAATTGTGTTTGTTCTCAAC+AGG - chr4_2:38971987-38972006 Msa0599420:CDS 35.0%
TTCTCAACAGGAATTTGAGA+AGG - chr4_2:38971999-38972018 Msa0599420:CDS 35.0%
!! AAGTACTGAGAGTTGTCTAT+GGG + chr4_2:38972031-38972050 None:intergenic 35.0%
!! AAAGTACTGAGAGTTGTCTA+TGG + chr4_2:38972032-38972051 None:intergenic 35.0%
!!! TTTTGTTATTCCAAAGCAGG+AGG + chr4_2:38972095-38972114 None:intergenic 35.0%
! GTGTTTTACCCCTGTAATAA+AGG - chr4_2:38972161-38972180 Msa0599420:intron 35.0%
GGAAATGACCTTTATTACAG+GGG + chr4_2:38972172-38972191 None:intergenic 35.0%
CGGAAATGACCTTTATTACA+GGG + chr4_2:38972173-38972192 None:intergenic 35.0%
CCTGTAATAAAGGTCATTTC+CGG - chr4_2:38972171-38972190 Msa0599420:intron 35.0%
GAAATTTCCTGAAATTACGG+GGG + chr4_2:38972281-38972300 None:intergenic 35.0%
CGAAATTTCCTGAAATTACG+GGG + chr4_2:38972282-38972301 None:intergenic 35.0%
CCGAAATTTCCTGAAATTAC+GGG + chr4_2:38972283-38972302 None:intergenic 35.0%
CCCGTAATTTCAGGAAATTT+CGG - chr4_2:38972280-38972299 Msa0599420:intron 35.0%
! CTGAAATTTGCTCAAATTGC+AGG + chr4_2:38972314-38972333 None:intergenic 35.0%
ACAAAATCGATGATATGGCA+GGG + chr4_2:38972373-38972392 None:intergenic 35.0%
TACAAAATCGATGATATGGC+AGG + chr4_2:38972374-38972393 None:intergenic 35.0%
! AAATTTTACAGGGGGAAAAC+TGG - chr4_2:38972459-38972478 Msa0599420:intron 35.0%
!! AAGCGACACTCATTTTGAAA+CGG + chr4_2:38972501-38972520 None:intergenic 35.0%
AAATTGTGTGAGCGAAAAGT+TGG + chr4_2:38972606-38972625 None:intergenic 35.0%
!!! CGACAGTTATTTTAAAACGG+AGG - chr4_2:38972739-38972758 Msa0599420:intron 35.0%
ATTTGGAGAAAATTGGGATG+GGG + chr4_2:38972871-38972890 None:intergenic 35.0%
TGAAAGTGGCTATGAGATTT+GGG - chr4_2:38973083-38973102 Msa0599420:intron 35.0%
! TGTCACTTCAGCGTAAAATT+TGG - chr4_2:38973124-38973143 Msa0599420:intron 35.0%
TCGATTCACCCGAATTAAAA+TGG + chr4_2:38973200-38973219 None:intergenic 35.0%
TATGTCAAACAACAGGTCAA+AGG - chr4_2:38973578-38973597 Msa0599420:CDS 35.0%
GAGCATATTTCCCTAAACAT+GGG + chr4_2:38973951-38973970 None:intergenic 35.0%
AGAGCATATTTCCCTAAACA+TGG + chr4_2:38973952-38973971 None:intergenic 35.0%
CATATTTCCCTTATGTTAGG+GGG + chr4_2:38973980-38973999 None:intergenic 35.0%
TTCTCAACAAGTTGCGATTA+TGG - chr4_2:38974018-38974037 Msa0599420:five_prime_UTR 35.0%
TACAAAATGGCTAGAGTAGG+AGG - chr4_2:38971631-38971650 Msa0599420:three_prime_UTR 40.0%
GGCTGAAATAGCACAAAACA+AGG - chr4_2:38971714-38971733 Msa0599420:three_prime_UTR 40.0%
AGGTTAAGCATTTGAAGGTG+TGG - chr4_2:38971860-38971879 Msa0599420:three_prime_UTR 40.0%
!! AACATGAAGGCATCATGACT+TGG + chr4_2:38971904-38971923 None:intergenic 40.0%
AAGTCATGATGCCTTCATGT+TGG - chr4_2:38971903-38971922 Msa0599420:CDS 40.0%
AGTCATGATGCCTTCATGTT+GGG - chr4_2:38971904-38971923 Msa0599420:CDS 40.0%
AGAGGCAGAGATATATTACC+AGG + chr4_2:38972067-38972086 None:intergenic 40.0%
CCGGAAATGACCTTTATTAC+AGG + chr4_2:38972174-38972193 None:intergenic 40.0%
! GGGGGCAAAATTTTTCATGA+AGG + chr4_2:38972263-38972282 None:intergenic 40.0%
! AATTTTGCCCCCGTAATTTC+AGG - chr4_2:38972271-38972290 Msa0599420:intron 40.0%
! AATTTGCTCAAATTGCAGGG+AGG + chr4_2:38972310-38972329 None:intergenic 40.0%
CAAAATCGATGATATGGCAG+GGG + chr4_2:38972372-38972391 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + chr4_2:38972497-38972516 None:intergenic 40.0%
AAAGTTGGAGAGGGTAAAGT+TGG + chr4_2:38972591-38972610 None:intergenic 40.0%
!! ATAACTGTCGCTTTAGGCTT+TGG + chr4_2:38972729-38972748 None:intergenic 40.0%
! AAACGGAGGGAGTATATTAC+AGG - chr4_2:38972753-38972772 Msa0599420:intron 40.0%
! AACGGAGGGAGTATATTACA+GGG - chr4_2:38972754-38972773 Msa0599420:intron 40.0%
! ATGGGGACAAGAAAGCATAA+GGG + chr4_2:38972854-38972873 None:intergenic 40.0%
GTGAAAGTGGCTATGAGATT+TGG - chr4_2:38973082-38973101 Msa0599420:intron 40.0%
CACTTCAGCGTAAAATTTGG+AGG - chr4_2:38973127-38973146 Msa0599420:intron 40.0%
ACTATGGTACGTAGTCTAAC+AGG + chr4_2:38973313-38973332 None:intergenic 40.0%
GATTGTCCATGCTGGTAATA+GGG - chr4_2:38973553-38973572 Msa0599420:CDS 40.0%
ATTGTCCATGCTGGTAATAG+GGG - chr4_2:38973554-38973573 Msa0599420:CDS 40.0%
AACAGGTCAAAGGATTCTTG+TGG - chr4_2:38973588-38973607 Msa0599420:CDS 40.0%
ATACTAATCCATGGAAGCAG+TGG + chr4_2:38973697-38973716 None:intergenic 40.0%
GCAAAAGAGACCCATGTTTA+GGG - chr4_2:38973938-38973957 Msa0599420:CDS 40.0%
! TCTTTTGACCCCCTAACATA+AGG - chr4_2:38973969-38973988 Msa0599420:exon 40.0%
! CTTTTGACCCCCTAACATAA+GGG - chr4_2:38973970-38973989 Msa0599420:five_prime_UTR 40.0%
!! CGCAACTTGTTGAGAAGATA+AGG + chr4_2:38974014-38974033 None:intergenic 40.0%
GTTATCATGGATGTTGCAGC+TGG - chr4_2:38971676-38971695 Msa0599420:three_prime_UTR 45.0%
GATGTTGCAGCTGGTATTCT+TGG - chr4_2:38971685-38971704 Msa0599420:three_prime_UTR 45.0%
!! AGCTGGTATTCTTGGCTTTG+AGG - chr4_2:38971693-38971712 Msa0599420:three_prime_UTR 45.0%
GTCATGATGCCTTCATGTTG+GGG - chr4_2:38971905-38971924 Msa0599420:CDS 45.0%
TACTGAGAGTTGTCTATGGG+AGG + chr4_2:38972028-38972047 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG + chr4_2:38972498-38972517 None:intergenic 45.0%
TGTGAGCGAAAAGTTGGAGA+GGG + chr4_2:38972600-38972619 None:intergenic 45.0%
! ACGGAGGGAGTATATTACAG+GGG - chr4_2:38972755-38972774 Msa0599420:intron 45.0%
! TGGGGACAAGAAAGCATAAG+GGG + chr4_2:38972853-38972872 None:intergenic 45.0%
! GATGGGGACAAGAAAGCATA+AGG + chr4_2:38972855-38972874 None:intergenic 45.0%
! TGTGTTGTTGATCAGGGTTG+TGG - chr4_2:38973521-38973540 Msa0599420:intron 45.0%
GGATTGTCCATGCTGGTAAT+AGG - chr4_2:38973552-38973571 Msa0599420:CDS 45.0%
TAGGGGCTATGTCAAACAAC+AGG - chr4_2:38973571-38973590 Msa0599420:CDS 45.0%
CACCATCACTTTCTGTCCAT+TGG - chr4_2:38973618-38973637 Msa0599420:CDS 45.0%
CACCAATGGACAGAAAGTGA+TGG + chr4_2:38973623-38973642 None:intergenic 45.0%
CATCACTTTCTGTCCATTGG+TGG - chr4_2:38973621-38973640 Msa0599420:CDS 45.0%
CATTGTTACCACTGCTTCCA+TGG - chr4_2:38973686-38973705 Msa0599420:CDS 45.0%
GGCAAAAGAGACCCATGTTT+AGG - chr4_2:38973937-38973956 Msa0599420:CDS 45.0%
TGATGCCTTCATGTTGGGGT+TGG - chr4_2:38971909-38971928 Msa0599420:CDS 50.0%
GATGCCTTCATGTTGGGGTT+GGG - chr4_2:38971910-38971929 Msa0599420:CDS 50.0%
! TTGGGTGCAGCAGTGCTATT+GGG - chr4_2:38971928-38971947 Msa0599420:CDS 50.0%
GTGTGAGCGAAAAGTTGGAG+AGG + chr4_2:38972601-38972620 None:intergenic 50.0%
! CGGAGGGAGTATATTACAGG+GGG - chr4_2:38972756-38972775 Msa0599420:intron 50.0%
CCACATTGGCCAAGAGAGTT+GGG + chr4_2:38972928-38972947 None:intergenic 50.0%
GTTGTTGATCAGGGTTGTGG+TGG - chr4_2:38973524-38973543 Msa0599420:intron 50.0%
CATAGCCCCTATTACCAGCA+TGG + chr4_2:38973562-38973581 None:intergenic 50.0%
! TGCGATTATGGCCCCTGTTT+TGG - chr4_2:38974030-38974049 Msa0599420:five_prime_UTR 50.0%
! GCGATTATGGCCCCTGTTTT+GGG - chr4_2:38974031-38974050 Msa0599420:five_prime_UTR 50.0%
TGCACCCAACCCCAACATGA+AGG + chr4_2:38971917-38971936 None:intergenic 55.0%
GTTGGGTGCAGCAGTGCTAT+TGG - chr4_2:38971927-38971946 Msa0599420:CDS 55.0%
CCAAAGCAGGAGGACATGAG+AGG + chr4_2:38972085-38972104 None:intergenic 55.0%
CCTCTCATGTCCTCCTGCTT+TGG - chr4_2:38972082-38972101 Msa0599420:intron 55.0%
GCCAAGAGAGTTGGGAGTCA+CGG + chr4_2:38972920-38972939 None:intergenic 55.0%
CCCAACTCTCTTGGCCAATG+TGG - chr4_2:38972925-38972944 Msa0599420:intron 55.0%
GCCACATTGGCCAAGAGAGT+TGG + chr4_2:38972929-38972948 None:intergenic 55.0%
ATCAGGGTTGTGGTGGCTGT+TGG - chr4_2:38973531-38973550 Msa0599420:intron 55.0%
GGCTGTTGGATTGTCCATGC+TGG - chr4_2:38973545-38973564 Msa0599420:CDS 55.0%
GCCGTGACTCCCAACTCTCT+TGG - chr4_2:38972916-38972935 Msa0599420:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 38971546 38974060 38971546 ID=Msa0599420;Name=Msa0599420
chr4_2 mRNA 38971546 38974060 38971546 ID=Msa0599420-mRNA-1;Parent=Msa0599420;Name=Msa0599420-mRNA-1;_AED=0.09;_eAED=0.09;_QI=91|1|1|1|1|1|3|347|160
chr4_2 exon 38971546 38972070 38971546 ID=Msa0599420-mRNA-1:exon:8558;Parent=Msa0599420-mRNA-1
chr4_2 exon 38973539 38973744 38973539 ID=Msa0599420-mRNA-1:exon:8557;Parent=Msa0599420-mRNA-1
chr4_2 exon 38973871 38974060 38973871 ID=Msa0599420-mRNA-1:exon:8556;Parent=Msa0599420-mRNA-1
chr4_2 five_prime_UTR 38973970 38974060 38973970 ID=Msa0599420-mRNA-1:five_prime_utr;Parent=Msa0599420-mRNA-1
chr4_2 CDS 38973871 38973969 38973871 ID=Msa0599420-mRNA-1:cds;Parent=Msa0599420-mRNA-1
chr4_2 CDS 38973539 38973744 38973539 ID=Msa0599420-mRNA-1:cds;Parent=Msa0599420-mRNA-1
chr4_2 CDS 38971893 38972070 38971893 ID=Msa0599420-mRNA-1:cds;Parent=Msa0599420-mRNA-1
chr4_2 three_prime_UTR 38971546 38971892 38971546 ID=Msa0599420-mRNA-1:three_prime_utr;Parent=Msa0599420-mRNA-1
Gene Sequence

>Msa0599420

ATGGCTAGAGTAGGAGGCATTGTTGTTTGTCTCCTAATTGTTATCATGGATGTTGCAGCTGGTATTCTTGGCTTTGAGGCTGAAATAGCACAAAACAAGGTTAAGCATTTGAAGGTGTGGATATTTGAGTGTAGAGAACCAAGTCATGATGCCTTCATGTTGGGGTTGGGTGCAGCAGTGCTATTGGGACTATCACATGTTTTAGCCAATTTGTTAGGTGGTTGCAATTGTGTTTGTTCTCAACAGGAATTTGAGAAGGCTTCCTCCCATAGACAACTCTCAGTACTTTGCCTTATTTTAACCTGGGTTGTGGTGGCTGTTGGATTGTCCATGCTGGTAATAGGGGCTATGTCAAACAACAGGTCAAAGGATTCTTGTGGTTTCTCACACCATCACTTTCTGTCCATTGGTGGAATTTCATGTTTTGTTCATGGTCTCTTCTCTGTTATATATTACATTGTTACCACTGCTTCCATGGATTAG
Protein sequence

>Msa0599420

MARVGGIVVCLLIVIMDVAAGILGFEAEIAQNKVKHLKVWIFECREPSHDAFMLGLGAAVLLGLSHVLANLLGGCNCVCSQQEFEKASSHRQLSVLCLILTWVVVAVGLSMLVIGAMSNNRSKDSCGFSHHHFLSIGGISCFVHGLFSVIYYIVTTASMD*