AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0996390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0996390 MtrunA17_Chr6g0481301 98.919 370 4 0 1 370 1 370 0.0 754
Msa0996390 MtrunA17_Chr5g0394641 33.673 98 51 4 37 130 19 106 5.91e-11 64.3
Msa0996390 MtrunA17_Chr1g0204981 29.197 137 85 4 32 166 18 144 6.52e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0996390 AT1G48405.1 33.835 266 146 3 41 306 7 242 1.88e-40 145
Msa0996390 AT3G17680.1 43.096 239 122 5 45 277 12 242 8.74e-36 133
Msa0996390 AT3G17680.3 43.096 239 122 5 45 277 44 274 1.48e-35 133
Msa0996390 AT3G17680.2 42.553 235 121 5 49 277 60 286 1.35e-33 128
Msa0996390 AT1G03470.2 36.905 84 48 2 34 115 13 93 2.25e-11 64.3
Msa0996390 AT1G03470.3 36.905 84 48 2 34 115 13 93 2.25e-11 64.3
Msa0996390 AT1G03470.1 36.905 84 48 2 34 115 13 93 2.25e-11 64.3
Msa0996390 AT1G03470.4 36.905 84 48 2 34 115 13 93 2.25e-11 64.3
Msa0996390 AT1G03080.3 33.028 109 57 5 32 137 17 112 6.02e-11 64.7
Msa0996390 AT1G03080.1 33.028 109 57 5 32 137 17 112 6.02e-11 64.7
Msa0996390 AT1G03080.2 33.028 109 57 5 32 137 17 112 6.02e-11 64.7

Find 91 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGCTTCTCACCAGCATTTC+TGG 0.111146 6_4:+74564195 Msa0996390:CDS
TTGATTTCTTCATGCATAAA+AGG 0.151774 6_4:+74564132 Msa0996390:CDS
GCTAGTAGTAGTAGATTTCT+AGG 0.275860 6_4:+74568682 Msa0996390:three_prime_UTR
CCTTGTGTAGAGGGGCTTAT+AGG 0.278491 6_4:+74564034 None:intergenic
GCCATTGGTTAAGGGTTCTA+TGG 0.286368 6_4:+74567937 Msa0996390:CDS
ACAAATGTTGCCATTGGTTA+AGG 0.287694 6_4:+74567928 Msa0996390:CDS
TCTTTCCACTTAAACCTTCT+CGG 0.324114 6_4:-74568451 None:intergenic
CAAAAGGACGTTCAACATTA+AGG 0.329958 6_4:-74568988 None:intergenic
AGAAGAGAGAGTCAAGAAAA+TGG 0.366420 6_4:+74568355 Msa0996390:CDS
GATTAGTTGATATCAGCTAT+TGG 0.376987 6_4:+74568861 Msa0996390:three_prime_UTR
TAGGTTACTAAGCATGTGTT+TGG 0.380988 6_4:+74568701 Msa0996390:three_prime_UTR
ATTAGTTGATATCAGCTATT+GGG 0.386796 6_4:+74568862 Msa0996390:three_prime_UTR
CAAATGTTGCCATTGGTTAA+GGG 0.388258 6_4:+74567929 Msa0996390:CDS
CAAGTTTCAACTTTGGAAGA+AGG 0.406416 6_4:+74567830 Msa0996390:CDS
TTCATATCCACAATAATGTT+GGG 0.408849 6_4:-74564081 None:intergenic
TCAAGGACAGATACATGGTT+TGG 0.408986 6_4:+74568297 Msa0996390:CDS
CTTTATAATTTGAAACTGAA+TGG 0.413446 6_4:+74569020 Msa0996390:three_prime_UTR
GAAGAGTTTGCTTGAGGTGT+TGG 0.416910 6_4:+74568081 Msa0996390:CDS
AGGATGAAAATGTTGGCAAT+TGG 0.428892 6_4:+74567632 Msa0996390:CDS
CTCTTCACAAGTTTCAACTT+TGG 0.429231 6_4:+74567823 Msa0996390:CDS
TGGTGGTAGATTTGGAAGAA+AGG 0.431166 6_4:+74567612 Msa0996390:intron
GTTCATATCCACAATAATGT+TGG 0.442137 6_4:-74564082 None:intergenic
GATCAAGAAGAAGTTATTAG+TGG 0.443016 6_4:+74567860 Msa0996390:CDS
GAGTTATAGAGTTAATAGTT+TGG 0.443249 6_4:+74568144 Msa0996390:CDS
CCACCAACAACATGCCCTCC+TGG 0.444437 6_4:+74564175 Msa0996390:CDS
GAAAGTATTGTGAGTCTTCG+AGG 0.448170 6_4:+74568044 Msa0996390:CDS
TTTGCCTCTAGTATTATAGC+TGG 0.449053 6_4:+74568893 Msa0996390:three_prime_UTR
TTATCAACAAATGTTGCCAT+TGG 0.462796 6_4:+74567922 Msa0996390:CDS
GAGTCAAGTTGATTCGGATA+TGG 0.463764 6_4:+74567886 Msa0996390:CDS
TGGTGTGAGTCAAGTTGATT+CGG 0.464121 6_4:+74567880 Msa0996390:CDS
TGATTTCTTCATGCATAAAA+GGG 0.465405 6_4:+74564133 Msa0996390:CDS
GGAAGAAAGGATGAAAATGT+TGG 0.467904 6_4:+74567625 Msa0996390:CDS
TGGTGGTGAAGGGATAGCTT+TGG 0.472422 6_4:+74568405 Msa0996390:CDS
TTTGGAAGTTCATGTTCAAA+TGG 0.473753 6_4:+74568423 Msa0996390:CDS
CAAGAAAATGGCGGTTGTAT+CGG 0.480959 6_4:+74568367 Msa0996390:CDS
TAAATACATACCAGAAATGC+TGG 0.485585 6_4:-74564205 None:intergenic
GGAAGAAAGGTCATAGTTTG+TGG 0.489302 6_4:+74568506 Msa0996390:CDS
GCCTCTAGTATTATAGCTGG+TGG 0.499964 6_4:+74568896 Msa0996390:three_prime_UTR
TATAAAGAAGTTGAAACAAA+AGG 0.499966 6_4:-74569004 None:intergenic
GGTTCATGAGGTTGGTGTCA+TGG 0.507161 6_4:+74568018 Msa0996390:CDS
AGGTTTAAGTGGAAAGAAGA+AGG 0.511084 6_4:+74568457 Msa0996390:CDS
TGCGCTTGATCTTGATCAGA+CGG 0.511806 6_4:+74568577 Msa0996390:exon
AGATACATGGTTTGGAGAAG+AGG 0.513312 6_4:+74568305 Msa0996390:CDS
CTTCTGCATGATTTGTACAA+TGG 0.516625 6_4:+74567737 Msa0996390:CDS
GGCGGTTGTATCGGCAACAA+AGG 0.518796 6_4:+74568376 Msa0996390:CDS
AAGAAGAAGGAAATTCATCT+AGG 0.520316 6_4:+74568470 Msa0996390:CDS
AGAAATGCTGGTGAGAAGCC+AGG 0.522647 6_4:-74564193 None:intergenic
AGGAAAATTGAAGATCTTCA+AGG 0.526049 6_4:+74568280 Msa0996390:CDS
TCTACAATTAGTATATCAAA+AGG 0.529520 6_4:-74568840 None:intergenic
GATCTTCAAGGACAGATACA+TGG 0.532234 6_4:+74568292 Msa0996390:CDS
AATCGCAGCAAAAGCATGGT+TGG 0.537808 6_4:-74568752 None:intergenic
GCAAAGGATATTGATGGAGA+AGG 0.539636 6_4:+74564056 Msa0996390:exon
ACAAGTGAAGATGAAGAAGA+AGG 0.541251 6_4:+74567656 Msa0996390:CDS
TTATAGGCAAAGGATATTGA+TGG 0.546816 6_4:+74564050 Msa0996390:exon
AAAGGGAAGAATGGTGGTGA+AGG 0.547748 6_4:+74568394 Msa0996390:CDS
ATAGAGTTTATCTATTGCAT+AGG 0.555558 6_4:+74568260 Msa0996390:CDS
CAAAGGATATTGATGGAGAA+GGG 0.558379 6_4:+74564057 Msa0996390:exon
GATCAAGATCAAGCGCAAGT+TGG 0.564528 6_4:-74568571 None:intergenic
CCAGGAGGGCATGTTGTTGG+TGG 0.564949 6_4:-74564175 None:intergenic
CAAAGAGCTTGCGTCCGAGT+CGG 0.566207 6_4:+74568177 Msa0996390:CDS
AAGCCAGGAGGGCATGTTGT+TGG 0.572709 6_4:-74564178 None:intergenic
AAGGGAAGAATGGTGGTGAA+GGG 0.574928 6_4:+74568395 Msa0996390:CDS
ATCGGCAACAAAGGGAAGAA+TGG 0.575270 6_4:+74568385 Msa0996390:CDS
TGCAGAAGTGAAAGTAGTTG+AGG 0.584459 6_4:-74567722 None:intergenic
AGAAGCCGAGAAGGTTTAAG+TGG 0.585872 6_4:+74568446 Msa0996390:CDS
GCGGTTGTATCGGCAACAAA+GGG 0.593239 6_4:+74568377 Msa0996390:CDS
TTCAAATGGAGAAGCCGAGA+AGG 0.593987 6_4:+74568437 Msa0996390:CDS
AACATGGACTTGTTGCTATG+TGG 0.596720 6_4:+74568541 Msa0996390:CDS
CTAAGTGTGTGCTGAAGATG+AGG 0.598254 6_4:+74568230 Msa0996390:CDS
GAAGGGACCCAACATTATTG+TGG 0.599311 6_4:+74564074 Msa0996390:CDS
TAGAAATCGCAGCAAAAGCA+TGG 0.604612 6_4:-74568756 None:intergenic
ACATGGACTTGTTGCTATGT+GGG 0.605405 6_4:+74568542 Msa0996390:CDS
ACCATAGAACCCTTAACCAA+TGG 0.605516 6_4:-74567938 None:intergenic
GTTACAGAAGAGTTTGCTTG+AGG 0.610285 6_4:+74568075 Msa0996390:CDS
AAGTGTTTGTATGTAACGAT+CGG 0.614205 6_4:-74567774 None:intergenic
TTGATCAGACGGTTCAGATG+TGG 0.617813 6_4:+74568588 Msa0996390:three_prime_UTR
TCTCTTGATGAAAACCGACT+CGG 0.619580 6_4:-74568191 None:intergenic
ATGCTGGTGAGAAGCCAGGA+GGG 0.624757 6_4:-74564189 None:intergenic
CACACACTTAGCTAACTCAC+CGG 0.626155 6_4:-74568218 None:intergenic
GTACTGTTGAATGAGAATGC+TGG 0.626374 6_4:+74568118 Msa0996390:CDS
ACCACGTGATTGTACGTAGT+CGG 0.637839 6_4:-74568611 None:intergenic
GTAGAGGGGCTTATAGGCAA+AGG 0.640600 6_4:+74564040 None:intergenic
ACCACCAGCTATAATACTAG+AGG 0.659287 6_4:-74568897 None:intergenic
GGAACAAGGAGTATTATGAG+AGG 0.665037 6_4:+74568326 Msa0996390:CDS
GGCAACAAAGGGAAGAATGG+TGG 0.678048 6_4:+74568388 Msa0996390:CDS
AATGCTGGTGAGAAGCCAGG+AGG 0.697605 6_4:-74564190 None:intergenic
TGGTTTGGAGAAGAGGAACA+AGG 0.704148 6_4:+74568312 Msa0996390:CDS
GCCGACTACGTACAATCACG+TGG 0.714108 6_4:+74568610 Msa0996390:three_prime_UTR
AGAGAGAGTCAAGAAAATGG+CGG 0.718855 6_4:+74568358 Msa0996390:CDS
GTGTGTGCTGAAGATGAGGG+AGG 0.735654 6_4:+74568234 Msa0996390:CDS
TAAGTGTGTGCTGAAGATGA+GGG 0.745974 6_4:+74568231 Msa0996390:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTGGAATATTTTTTTT+TGG + chr6_4:74564827-74564846 Msa0996390:intron 10.0%
!! AATAGTATCAATAATATTAA+AGG + chr6_4:74565449-74565468 Msa0996390:intron 10.0%
!! ATAGTATCAATAATATTAAA+GGG + chr6_4:74565450-74565469 Msa0996390:intron 10.0%
!!! AAATTTTGAAAAACAAAAAT+TGG - chr6_4:74566745-74566764 None:intergenic 10.0%
!!! AGTTGTATTTTTTATTTTTA+TGG + chr6_4:74567430-74567449 Msa0996390:intron 10.0%
!! AAAAAAAAAAATTGGCAATA+AGG - chr6_4:74564521-74564540 None:intergenic 15.0%
!!! AATATTTTTTTTTGGCAATA+AGG + chr6_4:74564835-74564854 Msa0996390:intron 15.0%
!! TTTAAATGGTTAATAAACTT+TGG - chr6_4:74565185-74565204 None:intergenic 15.0%
!! AATTTCTCATTTATTACTAT+AGG - chr6_4:74565389-74565408 None:intergenic 15.0%
!!! TATTTTTCTTTTTCCATTTA+AGG - chr6_4:74565425-74565444 None:intergenic 15.0%
!! TTAAATTCATATATCAATCT+AGG - chr6_4:74565782-74565801 None:intergenic 15.0%
!! ACAATATATAATATTACTTG+TGG - chr6_4:74566119-74566138 None:intergenic 15.0%
!!! GGTTTAAAATACTATATTAT+AGG - chr6_4:74566296-74566315 None:intergenic 15.0%
!!! AAAGAACTTTATTATAGTTT+TGG + chr6_4:74566776-74566795 Msa0996390:intron 15.0%
!! TATGACAAAAATAATAATGT+AGG - chr6_4:74567126-74567145 None:intergenic 15.0%
!! AAATAAAAAATTCTCTTACA+TGG - chr6_4:74567481-74567500 None:intergenic 15.0%
!!! TTTAATGTTTTTTTTCTTCA+TGG + chr6_4:74567528-74567547 Msa0996390:intron 15.0%
!! ACAAATTGAAAGACATAAAT+TGG + chr6_4:74564392-74564411 Msa0996390:intron 20.0%
!!! TAACTATGTTACAGATTTTA+AGG - chr6_4:74564419-74564438 None:intergenic 20.0%
!! TTAGGTTAATAATTCTCATA+CGG + chr6_4:74564801-74564820 Msa0996390:intron 20.0%
!! AGAAAAAAAATCTATTCTTG+AGG + chr6_4:74564860-74564879 Msa0996390:intron 20.0%
!! ACTAGTTATCTGAATTTAAA+TGG - chr6_4:74565199-74565218 None:intergenic 20.0%
!! AAAGGAAAAAAAAATCGATT+TGG - chr6_4:74565277-74565296 None:intergenic 20.0%
!! TAGAAAAATGTTGTATTGTA+AGG - chr6_4:74565547-74565566 None:intergenic 20.0%
!!! ATTTTTCTATCAATCATCAA+TGG + chr6_4:74565558-74565577 Msa0996390:intron 20.0%
!!! TTTTTCTATCAATCATCAAT+GGG + chr6_4:74565559-74565578 Msa0996390:intron 20.0%
!! AAAAATGTACTTTAGTAACA+TGG - chr6_4:74565716-74565735 None:intergenic 20.0%
!! AATCATATAAATCACATCAT+TGG - chr6_4:74566207-74566226 None:intergenic 20.0%
!! AATTAATCACAAATTAGACA+CGG - chr6_4:74566363-74566382 None:intergenic 20.0%
!!! AATGTAAGAATCTTTTTCAT+AGG + chr6_4:74566540-74566559 Msa0996390:intron 20.0%
!! AAAATTGGTATGTAAAGTTA+TGG - chr6_4:74566730-74566749 None:intergenic 20.0%
!!! TTTTCAAAATTTGAAACCAA+TGG + chr6_4:74566753-74566772 Msa0996390:intron 20.0%
!! TATAATAAAGTTCTTTCCAT+TGG - chr6_4:74566772-74566791 None:intergenic 20.0%
!! ACAAAAACCATAAATAATTC+TGG - chr6_4:74567180-74567199 None:intergenic 20.0%
!!! AGATTTATATGTGTACTTTT+TGG + chr6_4:74568650-74568669 Msa0996390:three_prime_UTR 20.0%
!! TCTACAATTAGTATATCAAA+AGG - chr6_4:74568843-74568862 None:intergenic 20.0%
!! TATAAAGAAGTTGAAACAAA+AGG - chr6_4:74569007-74569026 None:intergenic 20.0%
!!! CTTTATAATTTGAAACTGAA+TGG + chr6_4:74569020-74569039 Msa0996390:three_prime_UTR 20.0%
! TTCATATCCACAATAATGTT+GGG - chr6_4:74564084-74564103 None:intergenic 25.0%
! TTGATTTCTTCATGCATAAA+AGG + chr6_4:74564132-74564151 Msa0996390:CDS 25.0%
! TGATTTCTTCATGCATAAAA+GGG + chr6_4:74564133-74564152 Msa0996390:CDS 25.0%
! TGAAGAAAGTAATATAGGTA+CGG - chr6_4:74564241-74564260 None:intergenic 25.0%
! CATCATGAAGAAAGTAATAT+AGG - chr6_4:74564246-74564265 None:intergenic 25.0%
!! GTTAAATTAGCCCTTTTATA+AGG + chr6_4:74564435-74564454 Msa0996390:intron 25.0%
! TGGGTTAATAATTCTCATAA+GGG - chr6_4:74564556-74564575 None:intergenic 25.0%
! TTGGGTTAATAATTCTCATA+AGG - chr6_4:74564557-74564576 None:intergenic 25.0%
! ATGGCAATAAAAACTAAAAG+TGG + chr6_4:74564773-74564792 Msa0996390:intron 25.0%
! AAACTAAAAGTGGATCATTT+AGG + chr6_4:74564783-74564802 Msa0996390:intron 25.0%
! TAGGTTAATAATTCTCATAC+GGG + chr6_4:74564802-74564821 Msa0996390:intron 25.0%
! GTTAAGTAAACAAACAGAAA+TGG - chr6_4:74564992-74565011 None:intergenic 25.0%
! TTTCAACCAATATTTGATCT+CGG + chr6_4:74565133-74565152 Msa0996390:intron 25.0%
!!! CCTATTTGGTTTTTTTTGAA+AGG - chr6_4:74565295-74565314 None:intergenic 25.0%
! CCTTTCAAAAAAAACCAAAT+AGG + chr6_4:74565292-74565311 Msa0996390:intron 25.0%
! CTATATCAATGACATCAAAT+TGG - chr6_4:74565335-74565354 None:intergenic 25.0%
! CAATTTGATGTCATTGATAT+AGG + chr6_4:74565333-74565352 Msa0996390:intron 25.0%
! GTGAAGAAATCTGAATTAAA+TGG + chr6_4:74565485-74565504 Msa0996390:intron 25.0%
! TGGTTAATATAATGTGGTTA+TGG + chr6_4:74566075-74566094 Msa0996390:intron 25.0%
! ATATAATGTGGTTATGGTAT+AGG + chr6_4:74566081-74566100 Msa0996390:intron 25.0%
! TATAATGTGGTTATGGTATA+GGG + chr6_4:74566082-74566101 Msa0996390:intron 25.0%
! ACAATGTTGTAGAATAGTAT+AGG - chr6_4:74567069-74567088 None:intergenic 25.0%
! TGTACTACCAGAATTATTTA+TGG + chr6_4:74567170-74567189 Msa0996390:intron 25.0%
! CAAACAAAGACACAATAAAA+GGG - chr6_4:74567210-74567229 None:intergenic 25.0%
!!! AAGTTGTGTTTTTTTCTTCA+TGG + chr6_4:74567330-74567349 Msa0996390:intron 25.0%
!!! TTTTTATGGTTTTGTACCTA+TGG + chr6_4:74567444-74567463 Msa0996390:intron 25.0%
! ATGTGAACTTGGTTTATTTA+TGG + chr6_4:74567592-74567611 Msa0996390:intron 25.0%
! TTTATTTATGGTGGTAGATT+TGG + chr6_4:74567604-74567623 Msa0996390:intron 25.0%
!!! GAATGTTATGTGTGATTTTT+TGG + chr6_4:74567997-74568016 Msa0996390:CDS 25.0%
! GAGTTATAGAGTTAATAGTT+TGG + chr6_4:74568144-74568163 Msa0996390:CDS 25.0%
! ATAGAGTTTATCTATTGCAT+AGG + chr6_4:74568260-74568279 Msa0996390:CDS 25.0%
!! AAAATCAAGCTTTTAAGCTA+TGG + chr6_4:74568788-74568807 Msa0996390:three_prime_UTR 25.0%
!! ATTAGTTGATATCAGCTATT+GGG + chr6_4:74568862-74568881 Msa0996390:three_prime_UTR 25.0%
TTATAGGCAAAGGATATTGA+TGG + chr6_4:74564050-74564069 Msa0996390:exon 30.0%
GTTCATATCCACAATAATGT+TGG - chr6_4:74564085-74564104 None:intergenic 30.0%
TAAATACATACCAGAAATGC+TGG - chr6_4:74564208-74564227 None:intergenic 30.0%
ATGATGAAATTGTTTGAGTG+TGG + chr6_4:74564260-74564279 Msa0996390:intron 30.0%
GAGATTTGGACCTTATAAAA+GGG - chr6_4:74564448-74564467 None:intergenic 30.0%
AGAGATTTGGACCTTATAAA+AGG - chr6_4:74564449-74564468 None:intergenic 30.0%
AACCTTAAAGTAGGTCATTT+GGG - chr6_4:74564575-74564594 None:intergenic 30.0%
AAACCTTAAAGTAGGTCATT+TGG - chr6_4:74564576-74564595 None:intergenic 30.0%
TTCATTACAGCCAAATGAAA+TGG + chr6_4:74564611-74564630 Msa0996390:intron 30.0%
TTTAAAAAAAACGGCTGCTT+AGG + chr6_4:74564695-74564714 Msa0996390:intron 30.0%
CTATTCTTGAGGTACAATAT+TGG + chr6_4:74564871-74564890 Msa0996390:intron 30.0%
GAACAAATTCACACATCAAT+TGG - chr6_4:74565087-74565106 None:intergenic 30.0%
! ATTGATGTGTGAATTTGTTC+TGG + chr6_4:74565087-74565106 Msa0996390:intron 30.0%
ATGAATCCGAGATCAAATAT+TGG - chr6_4:74565142-74565161 None:intergenic 30.0%
AAAAGAAAGTGACACCTATT+TGG - chr6_4:74565309-74565328 None:intergenic 30.0%
TTTCAAAAGCTTGCCTTAAA+TGG + chr6_4:74565409-74565428 Msa0996390:intron 30.0%
CATTTCAGTATCATACAGTA+TGG - chr6_4:74565618-74565637 None:intergenic 30.0%
ACATGGTATACAATGATGTA+CGG - chr6_4:74565699-74565718 None:intergenic 30.0%
! GACACATTGTTTTCTAATAC+TGG + chr6_4:74566007-74566026 Msa0996390:intron 30.0%
!! TTGAAGTGTTGCTAATAGAT+TGG + chr6_4:74566052-74566071 Msa0996390:intron 30.0%
! GATTGGTGGTTAATATAATG+TGG + chr6_4:74566069-74566088 Msa0996390:intron 30.0%
ATTGTAGGATAACAAAGGTA+AGG - chr6_4:74566317-74566336 None:intergenic 30.0%
AACACATTGTAGGATAACAA+AGG - chr6_4:74566322-74566341 None:intergenic 30.0%
! ATCCTACAATGTGTTTGTTT+TGG + chr6_4:74566327-74566346 Msa0996390:intron 30.0%
ATCCAAAACAAACACATTGT+AGG - chr6_4:74566332-74566351 None:intergenic 30.0%
AACATGTGCTAAGAAAAAGT+TGG + chr6_4:74566512-74566531 Msa0996390:intron 30.0%
AATCTTGGTTGAAATCAGAT+TGG - chr6_4:74566626-74566645 None:intergenic 30.0%
TTATGGCAAAGGTTAAAGAA+AGG - chr6_4:74566713-74566732 None:intergenic 30.0%
GTACTTCAAATATGTTGATG+TGG - chr6_4:74566803-74566822 None:intergenic 30.0%
TGGGAAATGTGTAATTCTAA+CGG - chr6_4:74566865-74566884 None:intergenic 30.0%
ACAAGACATGAGGATAAAAT+GGG - chr6_4:74566884-74566903 None:intergenic 30.0%
GTTGTAGAATAGTATAGGTT+AGG - chr6_4:74567064-74567083 None:intergenic 30.0%
CCAAACAAAGACACAATAAA+AGG - chr6_4:74567211-74567230 None:intergenic 30.0%
! CCTTTTATTGTGTCTTTGTT+TGG + chr6_4:74567208-74567227 Msa0996390:intron 30.0%
ATATAGACCAGATCTCAATA+GGG - chr6_4:74567271-74567290 None:intergenic 30.0%
AATATAGACCAGATCTCAAT+AGG - chr6_4:74567272-74567291 None:intergenic 30.0%
! GGTCTATATTAACCTTCTTT+TGG + chr6_4:74567282-74567301 Msa0996390:intron 30.0%
!!! TCTTCATGGTTTTGAAGTTA+TGG + chr6_4:74567542-74567561 Msa0996390:intron 30.0%
TACTAACTCAAATGTGAACT+TGG + chr6_4:74567581-74567600 Msa0996390:intron 30.0%
TGAACTTGGTTTATTTATGG+TGG + chr6_4:74567595-74567614 Msa0996390:intron 30.0%
AAGTGTTTGTATGTAACGAT+CGG - chr6_4:74567777-74567796 None:intergenic 30.0%
GATCAAGAAGAAGTTATTAG+TGG + chr6_4:74567860-74567879 Msa0996390:CDS 30.0%
TTATCAACAAATGTTGCCAT+TGG + chr6_4:74567922-74567941 Msa0996390:CDS 30.0%
AGGAAAATTGAAGATCTTCA+AGG + chr6_4:74568280-74568299 Msa0996390:CDS 30.0%
TTTGGAAGTTCATGTTCAAA+TGG + chr6_4:74568423-74568442 Msa0996390:CDS 30.0%
AAGAAGAAGGAAATTCATCT+AGG + chr6_4:74568470-74568489 Msa0996390:CDS 30.0%
! GATTAGTTGATATCAGCTAT+TGG + chr6_4:74568861-74568880 Msa0996390:three_prime_UTR 30.0%
CAAAGGATATTGATGGAGAA+GGG + chr6_4:74564057-74564076 Msa0996390:exon 35.0%
TCAAATCAAACCCATCGTTT+AGG - chr6_4:74564472-74564491 None:intergenic 35.0%
AACCCAAATGACCTACTTTA+AGG + chr6_4:74564570-74564589 Msa0996390:intron 35.0%
!! GACCTACTTTAAGGTTTGTT+TGG + chr6_4:74564579-74564598 Msa0996390:intron 35.0%
GACCAAACAAACCTTAAAGT+AGG - chr6_4:74564584-74564603 None:intergenic 35.0%
!!! TTTTTTTTAAACAGCTGCCC+AGG - chr6_4:74564686-74564705 None:intergenic 35.0%
GGGCAGCTGTTTAAAAAAAA+CGG + chr6_4:74564686-74564705 Msa0996390:intron 35.0%
GTTACAGTCAAATGAAATGG+CGG - chr6_4:74564744-74564763 None:intergenic 35.0%
CTCGTTACAGTCAAATGAAA+TGG - chr6_4:74564747-74564766 None:intergenic 35.0%
ATTCTCATACGGGTCATAAT+TGG + chr6_4:74564812-74564831 Msa0996390:intron 35.0%
! GTGAATTTGTTCTGGCAATT+CGG + chr6_4:74565095-74565114 Msa0996390:intron 35.0%
ATCAATCATCAATGGGAAGA+AGG + chr6_4:74565566-74565585 Msa0996390:intron 35.0%
TCAATCATCAATGGGAAGAA+GGG + chr6_4:74565567-74565586 Msa0996390:intron 35.0%
TTCAGTATCATACAGTATGG+AGG - chr6_4:74565615-74565634 None:intergenic 35.0%
! AAGTGTTGCTAATAGATTGG+TGG + chr6_4:74566055-74566074 Msa0996390:intron 35.0%
AACAGATTGACACCATCATA+GGG - chr6_4:74566587-74566606 None:intergenic 35.0%
AAACAGATTGACACCATCAT+AGG - chr6_4:74566588-74566607 None:intergenic 35.0%
GTTGAAATCAGATTGGAGAT+TGG - chr6_4:74566619-74566638 None:intergenic 35.0%
! TGACAATGGATTTGGAATCT+TGG - chr6_4:74566641-74566660 None:intergenic 35.0%
! CAGAACAATGACAATGGATT+TGG - chr6_4:74566649-74566668 None:intergenic 35.0%
CAAATCCATTGTCATTGTTC+TGG + chr6_4:74566647-74566666 Msa0996390:intron 35.0%
GGTATGTAAAGTTATGGCAA+AGG - chr6_4:74566724-74566743 None:intergenic 35.0%
CACAAGACATGAGGATAAAA+TGG - chr6_4:74566885-74566904 None:intergenic 35.0%
TATAAAGTGCACAAGACATG+AGG - chr6_4:74566894-74566913 None:intergenic 35.0%
GCTATGAACTTGAGTAAATC+AGG + chr6_4:74567360-74567379 Msa0996390:intron 35.0%
CATGGTTAATTCAGTTCCAT+AGG - chr6_4:74567463-74567482 None:intergenic 35.0%
GGAAGAAAGGATGAAAATGT+TGG + chr6_4:74567625-74567644 Msa0996390:CDS 35.0%
AGGATGAAAATGTTGGCAAT+TGG + chr6_4:74567632-74567651 Msa0996390:CDS 35.0%
ACAAGTGAAGATGAAGAAGA+AGG + chr6_4:74567656-74567675 Msa0996390:CDS 35.0%
! CTTCTGCATGATTTGTACAA+TGG + chr6_4:74567737-74567756 Msa0996390:CDS 35.0%
CTCTTCACAAGTTTCAACTT+TGG + chr6_4:74567823-74567842 Msa0996390:CDS 35.0%
! CAAGTTTCAACTTTGGAAGA+AGG + chr6_4:74567830-74567849 Msa0996390:CDS 35.0%
! ACAAATGTTGCCATTGGTTA+AGG + chr6_4:74567928-74567947 Msa0996390:CDS 35.0%
! CAAATGTTGCCATTGGTTAA+GGG + chr6_4:74567929-74567948 Msa0996390:CDS 35.0%
!!! GTGTGATTTTTTGGTTCATG+AGG + chr6_4:74568006-74568025 Msa0996390:CDS 35.0%
!!! GATTTTTTGGTTCATGAGGT+TGG + chr6_4:74568010-74568029 Msa0996390:CDS 35.0%
! GTTTTCATCAAGAGAAAAGC+CGG + chr6_4:74568199-74568218 Msa0996390:CDS 35.0%
AGAAGAGAGAGTCAAGAAAA+TGG + chr6_4:74568355-74568374 Msa0996390:CDS 35.0%
TCTTTCCACTTAAACCTTCT+CGG - chr6_4:74568454-74568473 None:intergenic 35.0%
AGGTTTAAGTGGAAAGAAGA+AGG + chr6_4:74568457-74568476 Msa0996390:CDS 35.0%
! TCATCTAGGAAGAATTTTGC+TGG + chr6_4:74568484-74568503 Msa0996390:CDS 35.0%
! CATCTAGGAAGAATTTTGCT+GGG + chr6_4:74568485-74568504 Msa0996390:CDS 35.0%
! AAGAATTTTGCTGGGAAGAA+AGG + chr6_4:74568493-74568512 Msa0996390:CDS 35.0%
GCTAGTAGTAGTAGATTTCT+AGG + chr6_4:74568682-74568701 Msa0996390:three_prime_UTR 35.0%
TAGGTTACTAAGCATGTGTT+TGG + chr6_4:74568701-74568720 Msa0996390:three_prime_UTR 35.0%
TTTGCCTCTAGTATTATAGC+TGG + chr6_4:74568893-74568912 Msa0996390:three_prime_UTR 35.0%
CAAAAGGACGTTCAACATTA+AGG - chr6_4:74568991-74569010 None:intergenic 35.0%
GCAAAGGATATTGATGGAGA+AGG + chr6_4:74564056-74564075 Msa0996390:exon 40.0%
!! AGTGTGGACTAGAGAAATGT+GGG + chr6_4:74564276-74564295 Msa0996390:intron 40.0%
TCCAAATCTCTCCTAAACGA+TGG + chr6_4:74564458-74564477 Msa0996390:intron 40.0%
CCAAATCTCTCCTAAACGAT+GGG + chr6_4:74564459-74564478 Msa0996390:intron 40.0%
GCCAAATGAAATGGCTGTAA+TGG + chr6_4:74564620-74564639 Msa0996390:intron 40.0%
GCCATTACAGCCATTTCATT+TGG - chr6_4:74564624-74564643 None:intergenic 40.0%
! AATTTGTTCTGGCAATTCGG+AGG + chr6_4:74565098-74565117 Msa0996390:intron 40.0%
ACAAACGTCCAAGTCTTCTT+TGG + chr6_4:74565661-74565680 Msa0996390:intron 40.0%
!! TGCTGTTTCCAAAGAAGACT+TGG - chr6_4:74565672-74565691 None:intergenic 40.0%
CAATGTGTCTTCGACACATA+TGG - chr6_4:74565996-74566015 None:intergenic 40.0%
GTGCTAAGAAAAAGTTGGCA+TGG + chr6_4:74566517-74566536 Msa0996390:intron 40.0%
TTTCATAGGCTGCTATTGAC+AGG + chr6_4:74566554-74566573 Msa0996390:intron 40.0%
ACAGATTGACACCATCATAG+GGG - chr6_4:74566586-74566605 None:intergenic 40.0%
ATGAGCCAGAACAATGACAA+TGG - chr6_4:74566655-74566674 None:intergenic 40.0%
TCATCAACCCTATTGAGATC+TGG + chr6_4:74567261-74567280 Msa0996390:intron 40.0%
CCAATGAGAACACCAAAAGA+AGG - chr6_4:74567297-74567316 None:intergenic 40.0%
!!! CCTTCTTTTGGTGTTCTCAT+TGG + chr6_4:74567294-74567313 Msa0996390:intron 40.0%
TGGTGGTAGATTTGGAAGAA+AGG + chr6_4:74567612-74567631 Msa0996390:intron 40.0%
TGCAGAAGTGAAAGTAGTTG+AGG - chr6_4:74567725-74567744 None:intergenic 40.0%
!! TGGTGTGAGTCAAGTTGATT+CGG + chr6_4:74567880-74567899 Msa0996390:CDS 40.0%
!! GAGTCAAGTTGATTCGGATA+TGG + chr6_4:74567886-74567905 Msa0996390:CDS 40.0%
ACCATAGAACCCTTAACCAA+TGG - chr6_4:74567941-74567960 None:intergenic 40.0%
!! GAAAGTATTGTGAGTCTTCG+AGG + chr6_4:74568044-74568063 Msa0996390:CDS 40.0%
! GTTACAGAAGAGTTTGCTTG+AGG + chr6_4:74568075-74568094 Msa0996390:CDS 40.0%
GTACTGTTGAATGAGAATGC+TGG + chr6_4:74568118-74568137 Msa0996390:CDS 40.0%
TCTCTTGATGAAAACCGACT+CGG - chr6_4:74568194-74568213 None:intergenic 40.0%
TAAGTGTGTGCTGAAGATGA+GGG + chr6_4:74568231-74568250 Msa0996390:CDS 40.0%
GATCTTCAAGGACAGATACA+TGG + chr6_4:74568292-74568311 Msa0996390:CDS 40.0%
TCAAGGACAGATACATGGTT+TGG + chr6_4:74568297-74568316 Msa0996390:CDS 40.0%
! AGATACATGGTTTGGAGAAG+AGG + chr6_4:74568305-74568324 Msa0996390:CDS 40.0%
!! GGAACAAGGAGTATTATGAG+AGG + chr6_4:74568326-74568345 Msa0996390:CDS 40.0%
AGAGAGAGTCAAGAAAATGG+CGG + chr6_4:74568358-74568377 Msa0996390:CDS 40.0%
CAAGAAAATGGCGGTTGTAT+CGG + chr6_4:74568367-74568386 Msa0996390:CDS 40.0%
GGAAGAAAGGTCATAGTTTG+TGG + chr6_4:74568506-74568525 Msa0996390:CDS 40.0%
TAGAAATCGCAGCAAAAGCA+TGG - chr6_4:74568759-74568778 None:intergenic 40.0%
ACCACCAGCTATAATACTAG+AGG - chr6_4:74568900-74568919 None:intergenic 40.0%
GAAGGGACCCAACATTATTG+TGG + chr6_4:74564074-74564093 Msa0996390:CDS 45.0%
!! GAGTGTGGACTAGAGAAATG+TGG + chr6_4:74564275-74564294 Msa0996390:intron 45.0%
!! GTGTGGACTAGAGAAATGTG+GGG + chr6_4:74564277-74564296 Msa0996390:intron 45.0%
CCCATCGTTTAGGAGAGATT+TGG - chr6_4:74564462-74564481 None:intergenic 45.0%
CAGAACACGACACGTGTTAT+GGG + chr6_4:74564643-74564662 Msa0996390:intron 45.0%
!!! TGTTATGGGCAGCCGTTTTT+TGG + chr6_4:74564657-74564676 Msa0996390:intron 45.0%
GTCAAATGAAATGGCGGCAA+TGG - chr6_4:74564738-74564757 None:intergenic 45.0%
TGACTGTAACGAGCACTACA+TGG + chr6_4:74564754-74564773 Msa0996390:intron 45.0%
ATCAATGGGAAGAAGGGATG+AGG + chr6_4:74565573-74565592 Msa0996390:intron 45.0%
TCAATGGGAAGAAGGGATGA+GGG + chr6_4:74565574-74565593 Msa0996390:intron 45.0%
ACAGGTATCTACCCCTATGA+TGG + chr6_4:74566572-74566591 Msa0996390:intron 45.0%
! GCCATTGGTTAAGGGTTCTA+TGG + chr6_4:74567937-74567956 Msa0996390:CDS 45.0%
! GAAGAGTTTGCTTGAGGTGT+TGG + chr6_4:74568081-74568100 Msa0996390:CDS 45.0%
CACACACTTAGCTAACTCAC+CGG - chr6_4:74568221-74568240 None:intergenic 45.0%
CTAAGTGTGTGCTGAAGATG+AGG + chr6_4:74568230-74568249 Msa0996390:CDS 45.0%
! TGGTTTGGAGAAGAGGAACA+AGG + chr6_4:74568312-74568331 Msa0996390:CDS 45.0%
ATCGGCAACAAAGGGAAGAA+TGG + chr6_4:74568385-74568404 Msa0996390:CDS 45.0%
! AAAGGGAAGAATGGTGGTGA+AGG + chr6_4:74568394-74568413 Msa0996390:CDS 45.0%
! AAGGGAAGAATGGTGGTGAA+GGG + chr6_4:74568395-74568414 Msa0996390:CDS 45.0%
TTCAAATGGAGAAGCCGAGA+AGG + chr6_4:74568437-74568456 Msa0996390:CDS 45.0%
AGAAGCCGAGAAGGTTTAAG+TGG + chr6_4:74568446-74568465 Msa0996390:CDS 45.0%
GATCAAGATCAAGCGCAAGT+TGG - chr6_4:74568574-74568593 None:intergenic 45.0%
TGCGCTTGATCTTGATCAGA+CGG + chr6_4:74568577-74568596 Msa0996390:exon 45.0%
TTGATCAGACGGTTCAGATG+TGG + chr6_4:74568588-74568607 Msa0996390:three_prime_UTR 45.0%
ACCACGTGATTGTACGTAGT+CGG - chr6_4:74568614-74568633 None:intergenic 45.0%
! AATCGCAGCAAAAGCATGGT+TGG - chr6_4:74568755-74568774 None:intergenic 45.0%
GCCTCTAGTATTATAGCTGG+TGG + chr6_4:74568896-74568915 Msa0996390:three_prime_UTR 45.0%
!! AAATGAAAAAAAAAAAAAAT+TGG - chr6_4:74564529-74564548 None:intergenic 5.0%
AGAAATGCTGGTGAGAAGCC+AGG - chr6_4:74564196-74564215 None:intergenic 50.0%
TGGCTTCTCACCAGCATTTC+TGG + chr6_4:74564195-74564214 Msa0996390:CDS 50.0%
GCAGAACACGACACGTGTTA+TGG + chr6_4:74564642-74564661 Msa0996390:intron 50.0%
!!! CAGCCGTTTTTTGGTGTCCT+GGG + chr6_4:74564666-74564685 Msa0996390:intron 50.0%
CTGCCCAGGACACCAAAAAA+CGG - chr6_4:74564672-74564691 None:intergenic 50.0%
TGTTCTGGCAATTCGGAGGT+CGG + chr6_4:74565102-74565121 Msa0996390:intron 50.0%
! AGAGAAGCGCGTGACAACAT+GGG - chr6_4:74565750-74565769 None:intergenic 50.0%
! AAGAGAAGCGCGTGACAACA+TGG - chr6_4:74565751-74565770 None:intergenic 50.0%
GCACTTAGCACATGCATCTC+AGG + chr6_4:74566957-74566976 Msa0996390:intron 50.0%
!! GGTTCATGAGGTTGGTGTCA+TGG + chr6_4:74568018-74568037 Msa0996390:CDS 50.0%
! GCGGTTGTATCGGCAACAAA+GGG + chr6_4:74568377-74568396 Msa0996390:CDS 50.0%
GGCAACAAAGGGAAGAATGG+TGG + chr6_4:74568388-74568407 Msa0996390:CDS 50.0%
! TGGTGGTGAAGGGATAGCTT+TGG + chr6_4:74568405-74568424 Msa0996390:CDS 50.0%
! AAGCCAGGAGGGCATGTTGT+TGG - chr6_4:74564181-74564200 None:intergenic 55.0%
ATGCTGGTGAGAAGCCAGGA+GGG - chr6_4:74564192-74564211 None:intergenic 55.0%
AATGCTGGTGAGAAGCCAGG+AGG - chr6_4:74564193-74564212 None:intergenic 55.0%
!!! GCAGCCGTTTTTTGGTGTCC+TGG + chr6_4:74564665-74564684 Msa0996390:intron 55.0%
GTTCTGGCAATTCGGAGGTC+GGG + chr6_4:74565103-74565122 Msa0996390:intron 55.0%
ATGGGAAGAAGGGATGAGGG+AGG + chr6_4:74565577-74565596 Msa0996390:intron 55.0%
GAAGAAGGGATGAGGGAGGA+AGG + chr6_4:74565581-74565600 Msa0996390:intron 55.0%
! GAGAAGCGCGTGACAACATG+GGG - chr6_4:74565749-74565768 None:intergenic 55.0%
CAAAGAGCTTGCGTCCGAGT+CGG + chr6_4:74568177-74568196 Msa0996390:CDS 55.0%
GTGTGTGCTGAAGATGAGGG+AGG + chr6_4:74568234-74568253 Msa0996390:CDS 55.0%
! GGCGGTTGTATCGGCAACAA+AGG + chr6_4:74568376-74568395 Msa0996390:CDS 55.0%
GCCGACTACGTACAATCACG+TGG + chr6_4:74568610-74568629 Msa0996390:three_prime_UTR 55.0%
! CCAGGAGGGCATGTTGTTGG+TGG - chr6_4:74564178-74564197 None:intergenic 60.0%
CCACCAACAACATGCCCTCC+TGG + chr6_4:74564175-74564194 Msa0996390:CDS 60.0%
GCTGCTTAGGACACGTGTCG+TGG + chr6_4:74564708-74564727 Msa0996390:intron 60.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 74564044 74569061 74564044 ID=Msa0996390;Name=Msa0996390
chr6_4 mRNA 74564044 74569061 74564044 ID=Msa0996390-mRNA-1;Parent=Msa0996390;Name=Msa0996390-mRNA-1;_AED=0.08;_eAED=0.08;_QI=25|1|0.5|1|1|1|2|476|370
chr6_4 exon 74564044 74564216 74564044 ID=Msa0996390-mRNA-1:exon:13943;Parent=Msa0996390-mRNA-1
chr6_4 exon 74567621 74569061 74567621 ID=Msa0996390-mRNA-1:exon:13944;Parent=Msa0996390-mRNA-1
chr6_4 five_prime_UTR 74564044 74564068 74564044 ID=Msa0996390-mRNA-1:five_prime_utr;Parent=Msa0996390-mRNA-1
chr6_4 CDS 74564069 74564216 74564069 ID=Msa0996390-mRNA-1:cds;Parent=Msa0996390-mRNA-1
chr6_4 CDS 74567621 74568585 74567621 ID=Msa0996390-mRNA-1:cds;Parent=Msa0996390-mRNA-1
chr6_4 three_prime_UTR 74568586 74569061 74568586 ID=Msa0996390-mRNA-1:three_prime_utr;Parent=Msa0996390-mRNA-1
Gene Sequence

>Msa0996390

ATGGAGAAGGGACCCAACATTATTGTGGATATGAACAAAGACAAAGTGATTTCAAATTCTCAATTGATTTCTTCATGCATAAAAGGGTTTAAAGACAAGAACATCACCACCAACAACATGCCCTCCTGGCTTCTCACCAGCATTTCTGATTTGGAAGAAAGGATGAAAATGTTGGCAATTGGAACAAGTGAAGATGAAGAAGAAGGTGACACTTTTGCTGAAAGAGCAGAAACTTACTATCAAAAGAGACCTCAACTACTTTCACTTCTGCATGATTTGTACAATGGCTATGTCACTTTATCCGATCGTTACATACAAACACTTGCGAAGCATAAGCATCATAGTAGACACTCTTCACAAGTTTCAACTTTGGAAGAAGGATTTTCTGATCAAGAAGAAGTTATTAGTGGTGTGAGTCAAGTTGATTCGGATATGGAAAGTTCAATCTCTTATCAACAAATGTTGCCATTGGTTAAGGGTTCTATGGTTGATGTTGATGAAATTGTAGCTGAGCTTGTGATGAAGAATGTTATGTGTGATTTTTTGGTTCATGAGGTTGGTGTCATGGAGAGAAAGTATTGTGAGTCTTCGAGGAAAAGTGAGTTACAGAAGAGTTTGCTTGAGGTGTTGGAATCTGAGAGACTTGTACTGTTGAATGAGAATGCTGGTTTGAGTTATAGAGTTAATAGTTTGGTTGAAGAGAACAAAGAGCTTGCGTCCGAGTCGGTTTTCATCAAGAGAAAAGCCGGTGAGTTAGCTAAGTGTGTGCTGAAGATGAGGGAGGATCATAGAGTTTATCTATTGCATAGGAAAATTGAAGATCTTCAAGGACAGATACATGGTTTGGAGAAGAGGAACAAGGAGTATTATGAGAGGCTTTTGAGAAGAGAGAGTCAAGAAAATGGCGGTTGTATCGGCAACAAAGGGAAGAATGGTGGTGAAGGGATAGCTTTGGAAGTTCATGTTCAAATGGAGAAGCCGAGAAGGTTTAAGTGGAAAGAAGAAGGAAATTCATCTAGGAAGAATTTTGCTGGGAAGAAAGGTCATAGTTTGTGGAAAAAGTTGAAGAACATGGACTTGTTGCTATGTGGGACAAATCCAACTTGCGCTTGA
Protein sequence

>Msa0996390

MEKGPNIIVDMNKDKVISNSQLISSCIKGFKDKNITTNNMPSWLLTSISDLEERMKMLAIGTSEDEEEGDTFAERAETYYQKRPQLLSLLHDLYNGYVTLSDRYIQTLAKHKHHSRHSSQVSTLEEGFSDQEEVISGVSQVDSDMESSISYQQMLPLVKGSMVDVDEIVAELVMKNVMCDFLVHEVGVMERKYCESSRKSELQKSLLEVLESERLVLLNENAGLSYRVNSLVEENKELASESVFIKRKAGELAKCVLKMREDHRVYLLHRKIEDLQGQIHGLEKRNKEYYERLLRRESQENGGCIGNKGKNGGEGIALEVHVQMEKPRRFKWKEEGNSSRKNFAGKKGHSLWKKLKNMDLLLCGTNPTCA*