AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1201890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1201890 MtrunA17_Chr8g0372141 96.891 193 6 0 1 193 1 193 9.91e-141 390
Msa1201890 MtrunA17_Chr5g0405171 78.462 195 39 3 1 193 14 207 7.34e-110 312
Msa1201890 MtrunA17_Chr2g0277151 63.514 148 48 2 12 159 2 143 2.74e-64 196
Msa1201890 MtrunA17_Chr4g0063501 57.639 144 57 1 12 155 4 143 2.88e-56 175
Msa1201890 MtrunA17_Chr4g0003581 54.362 149 61 2 12 157 8 152 2.25e-55 173
Msa1201890 MtrunA17_Chr4g0020201 47.887 142 72 2 14 154 35 175 1.20e-47 154
Msa1201890 MtrunA17_Chr2g0332851 49.333 150 68 3 12 157 8 153 4.90e-47 152
Msa1201890 MtrunA17_Chr2g0332841 48.667 150 69 3 12 157 8 153 2.04e-45 147
Msa1201890 MtrunA17_Chr1g0212241 44.444 135 66 3 30 161 3 131 3.96e-36 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1201890 AT1G15380.2 57.718 149 56 2 12 157 8 152 1.88e-58 181
Msa1201890 AT1G15380.1 57.718 149 56 2 12 157 8 152 1.88e-58 181
Msa1201890 AT1G80160.1 52.349 149 64 2 12 157 8 152 1.28e-54 171
Msa1201890 AT1G80160.3 52.349 149 64 2 12 157 49 193 3.62e-54 171
Msa1201890 AT2G28420.1 50.000 144 68 2 14 156 17 157 6.37e-49 157
Msa1201890 AT1G80160.2 48.077 104 47 2 57 157 27 126 1.74e-31 111

Find 72 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCAAATTCATCATAATT+TGG 0.184282 8_1:+55171800 None:intergenic
TTGTGTAGGTTGTATAATTA+TGG 0.227214 8_1:-55171848 Msa1201890:intron
ATGGTTGTCCTTGGGATTAA+TGG 0.258018 8_1:+55171757 None:intergenic
TATGAAGAAGTCTTGGGTTT+TGG 0.266509 8_1:-55172018 Msa1201890:CDS
TAATTACTTGGAATGATATA+TGG 0.268558 8_1:+55171738 None:intergenic
TTCTGCTTCCTGCTCTTTCA+AGG 0.282455 8_1:-55171462 Msa1201890:CDS
TTATTCATATTGTTGAAATA+AGG 0.292446 8_1:-55171334 Msa1201890:three_prime_UTR
ATTTAGTGGCTGGGTATATA+TGG 0.299391 8_1:+55170999 None:intergenic
TAAGGGAGAATATTGGAAAA+TGG 0.308317 8_1:-55172139 Msa1201890:exon
TGTGTAGGTTGTATAATTAT+GGG 0.310343 8_1:-55171847 Msa1201890:intron
CAACACTGATCTGCATAATA+TGG 0.311667 8_1:+55172056 None:intergenic
TTCTAACAAGTGTGGGTTCA+TGG 0.316320 8_1:-55171411 Msa1201890:CDS
CACGATATACAACATAGTTT+TGG 0.316398 8_1:+55171197 None:intergenic
GCAGAAGAAATGGGAACTAT+TGG 0.335608 8_1:+55171479 None:intergenic
ATGGGTCGTGATTCACTCAT+AGG 0.351235 8_1:+55171776 None:intergenic
GAAAATGAGGTTGGAAGAAA+TGG 0.352191 8_1:-55171612 Msa1201890:CDS
TCAAACTATACAATTTATAT+AGG 0.356737 8_1:+55171066 None:intergenic
CCTTCCTCTTTCAAATTCAA+TGG 0.370841 8_1:-55171982 Msa1201890:CDS
AAGGGAGAATATTGGAAAAT+GGG 0.376216 8_1:-55172138 Msa1201890:exon
AGAGCAGGAAGCAGAAGAAA+TGG 0.381171 8_1:+55171469 None:intergenic
AAAATGGGGATTGAGGAAAT+TGG 0.400288 8_1:-55172123 Msa1201890:CDS
TGGTTGTCCTTGGGATTAAT+GGG 0.407514 8_1:+55171758 None:intergenic
GATGAAGTATGTTACAGCTT+TGG 0.428272 8_1:-55171588 Msa1201890:CDS
CAGAAGAAATGGGAACTATT+GGG 0.429676 8_1:+55171480 None:intergenic
AGGTTGTATAATTATGGGTT+TGG 0.430257 8_1:-55171842 Msa1201890:CDS
GAGGTTCTATGAAGAAGTCT+TGG 0.431037 8_1:-55172025 Msa1201890:CDS
AGTTCTATCATGTATCCATC+AGG 0.435736 8_1:+55171521 None:intergenic
AAACAGTCTTCTTAAAGCTC+TGG 0.447084 8_1:+55171432 None:intergenic
GAATGATATATGGTTGTCCT+TGG 0.451197 8_1:+55171748 None:intergenic
AAAATGAGGTTGGAAGAAAT+GGG 0.458153 8_1:-55171611 Msa1201890:CDS
AGGTTCTATGAAGAAGTCTT+GGG 0.458934 8_1:-55172024 Msa1201890:CDS
TTGTGCAGTGTACAGATGTT+GGG 0.460233 8_1:-55171640 Msa1201890:intron
TGCTCCATTGAATTTGAAAG+AGG 0.466642 8_1:+55171978 None:intergenic
CACATGATTCAGTGAGAGAA+GGG 0.472092 8_1:+55172080 None:intergenic
TTTGTGCAGTGTACAGATGT+TGG 0.472216 8_1:-55171641 Msa1201890:intron
GAGCAGGAAGCAGAAGAAAT+GGG 0.476522 8_1:+55171470 None:intergenic
CTGAGTGAAGAAGTGTCCTA+AGG 0.490013 8_1:-55172157 Msa1201890:five_prime_UTR
TGAAGGAATCAAGGTGGAGC+AGG 0.491848 8_1:-55171558 Msa1201890:CDS
AGTGTCCTAAGGGAGAATAT+TGG 0.492635 8_1:-55172146 Msa1201890:five_prime_UTR
TGGGTCGTGATTCACTCATA+GGG 0.496984 8_1:+55171777 None:intergenic
TGTTCTTCAAGACAAGGAAA+AGG 0.517132 8_1:-55171258 Msa1201890:three_prime_UTR
TCATGTATCCATCAGGGTCA+TGG 0.518219 8_1:+55171528 None:intergenic
ACAGCTTTGGTAGAGGATGA+AGG 0.518692 8_1:-55171575 Msa1201890:CDS
CCATTGAATTTGAAAGAGGA+AGG 0.520942 8_1:+55171982 None:intergenic
AATGATATATGGTTGTCCTT+GGG 0.521545 8_1:+55171749 None:intergenic
GCCTCGTGCCTTGAAAGAGC+AGG 0.526263 8_1:+55171454 None:intergenic
CTTTCAAATTCAATGGAGCA+TGG 0.540814 8_1:-55171975 Msa1201890:intron
GTATGTTACAGCTTTGGTAG+AGG 0.543537 8_1:-55171582 Msa1201890:CDS
TGAGTGAAGAAGTGTCCTAA+GGG 0.543669 8_1:-55172156 Msa1201890:five_prime_UTR
TTCAGTGAGAGAAGGGGTAG+TGG 0.543925 8_1:+55172087 None:intergenic
TGGGCTAGTGAAAATGAGGT+TGG 0.545299 8_1:-55171621 Msa1201890:CDS
GGTAGAGGATGAAGGAATCA+AGG 0.549754 8_1:-55171567 Msa1201890:CDS
CATATTATGCAGATCAGTGT+TGG 0.552916 8_1:-55172055 Msa1201890:CDS
GTGTTCTTCCATGACCCTGA+TGG 0.553401 8_1:-55171536 Msa1201890:CDS
AAGACTGTTTCTAACAAGTG+TGG 0.559960 8_1:-55171419 Msa1201890:CDS
ATATTGGAAAATGGGGATTG+AGG 0.566391 8_1:-55172130 Msa1201890:exon
AGACTGTTTCTAACAAGTGT+GGG 0.576406 8_1:-55171418 Msa1201890:CDS
AGGGAGAATATTGGAAAATG+GGG 0.577637 8_1:-55172137 Msa1201890:exon
GATCAGTGTTGGACTCTATG+AGG 0.578559 8_1:-55172044 Msa1201890:CDS
AGTGTTTGTTCTTCAAGACA+AGG 0.586875 8_1:-55171264 Msa1201890:three_prime_UTR
TCCTGCTCTTTCAAGGCACG+AGG 0.591048 8_1:-55171455 Msa1201890:CDS
ACACATGATTCAGTGAGAGA+AGG 0.601179 8_1:+55172079 None:intergenic
GTTCTATCATGTATCCATCA+GGG 0.630489 8_1:+55171522 None:intergenic
CATTGAATTTGAAAGAGGAA+GGG 0.632398 8_1:+55171983 None:intergenic
TATGATGCGAAGCTTGAGCA+TGG 0.640468 8_1:-55171381 Msa1201890:CDS
ATCACGACCCATTAATCCCA+AGG 0.645670 8_1:-55171765 Msa1201890:CDS
ATGTTGGGCTAGTGAAAATG+AGG 0.654745 8_1:-55171625 Msa1201890:CDS
GGGTCGTGATTCACTCATAG+GGG 0.689430 8_1:+55171778 None:intergenic
AAATGAGGTTGGAAGAAATG+GGG 0.690532 8_1:-55171610 Msa1201890:CDS
ATAAATTGTATAGTTTGACA+AGG 0.690700 8_1:-55171061 Msa1201890:three_prime_UTR
ACATGATTCAGTGAGAGAAG+GGG 0.696257 8_1:+55172081 None:intergenic
AGAGGATGAAGGAATCAAGG+TGG 0.720837 8_1:-55171564 Msa1201890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAGTTTCATAAAGAAAA+TGG + chr8_1:55171278-55171297 None:intergenic 15.0%
!!! AAACTTTATATCCATTATTT+TGG - chr8_1:55171289-55171308 Msa1201890:three_prime_UTR 15.0%
!! TTATTCATATTGTTGAAATA+AGG - chr8_1:55171866-55171885 Msa1201890:intron 15.0%
!! TCAAACTATACAATTTATAT+AGG + chr8_1:55172137-55172156 None:intergenic 15.0%
!! TACCAAAAAACTTATTAAAT+AGG - chr8_1:55172173-55172192 Msa1201890:five_prime_UTR 15.0%
!! ATAAAGTTTCATAAAGAAAA+TGG + chr8_1:55171278-55171297 None:intergenic 15.0%
!!! AAACTTTATATCCATTATTT+TGG - chr8_1:55171289-55171308 Msa1201890:three_prime_UTR 15.0%
!! TTATTCATATTGTTGAAATA+AGG - chr8_1:55171866-55171885 Msa1201890:intron 15.0%
!! TCAAACTATACAATTTATAT+AGG + chr8_1:55172137-55172156 None:intergenic 15.0%
!! TACCAAAAAACTTATTAAAT+AGG - chr8_1:55172173-55172192 Msa1201890:five_prime_UTR 15.0%
!!! TTTTTTTTTACGTATTAGCT+TGG + chr8_1:55171017-55171036 None:intergenic 20.0%
!! TATTTGTTCAACCAAAATAA+TGG + chr8_1:55171303-55171322 None:intergenic 20.0%
!! ACACAAAACAAAACAAAAAA+TGG + chr8_1:55171338-55171357 None:intergenic 20.0%
!!! TTTTTTGTTTTGTTTTGTGT+AGG - chr8_1:55171338-55171357 Msa1201890:three_prime_UTR 20.0%
!! TAATTACTTGGAATGATATA+TGG + chr8_1:55171465-55171484 None:intergenic 20.0%
!!! TTACTAAAAGTGTTTCAATT+TGG - chr8_1:55171535-55171554 Msa1201890:CDS 20.0%
!!! ACTTGTAACAAATTTTTAAC+AGG - chr8_1:55172067-55172086 Msa1201890:CDS 20.0%
!!! CTTGTAACAAATTTTTAACA+GGG - chr8_1:55172068-55172087 Msa1201890:CDS 20.0%
!!! TTGTAACAAATTTTTAACAG+GGG - chr8_1:55172069-55172088 Msa1201890:CDS 20.0%
!! ATAAATTGTATAGTTTGACA+AGG - chr8_1:55172139-55172158 Msa1201890:exon 20.0%
!!! CACCTATTTAATAAGTTTTT+TGG + chr8_1:55172178-55172197 None:intergenic 20.0%
!! CAAAAAACTTATTAAATAGG+TGG - chr8_1:55172176-55172195 Msa1201890:five_prime_UTR 20.0%
!!! TTTTTTTTTACGTATTAGCT+TGG + chr8_1:55171017-55171036 None:intergenic 20.0%
!! TATTTGTTCAACCAAAATAA+TGG + chr8_1:55171303-55171322 None:intergenic 20.0%
!! ACACAAAACAAAACAAAAAA+TGG + chr8_1:55171338-55171357 None:intergenic 20.0%
!!! TTTTTTGTTTTGTTTTGTGT+AGG - chr8_1:55171338-55171357 Msa1201890:three_prime_UTR 20.0%
!! TAATTACTTGGAATGATATA+TGG + chr8_1:55171465-55171484 None:intergenic 20.0%
!!! TTACTAAAAGTGTTTCAATT+TGG - chr8_1:55171535-55171554 Msa1201890:CDS 20.0%
!!! ACTTGTAACAAATTTTTAAC+AGG - chr8_1:55172067-55172086 Msa1201890:CDS 20.0%
!!! CTTGTAACAAATTTTTAACA+GGG - chr8_1:55172068-55172087 Msa1201890:CDS 20.0%
!!! TTGTAACAAATTTTTAACAG+GGG - chr8_1:55172069-55172088 Msa1201890:CDS 20.0%
!! ATAAATTGTATAGTTTGACA+AGG - chr8_1:55172139-55172158 Msa1201890:exon 20.0%
!!! CACCTATTTAATAAGTTTTT+TGG + chr8_1:55172178-55172197 None:intergenic 20.0%
!! CAAAAAACTTATTAAATAGG+TGG - chr8_1:55172176-55172195 Msa1201890:five_prime_UTR 20.0%
! GGTAATAAAGCAAAGTATAT+AGG + chr8_1:55171257-55171276 None:intergenic 25.0%
! TTGTGTAGGTTGTATAATTA+TGG - chr8_1:55171352-55171371 Msa1201890:exon 25.0%
! TGTGTAGGTTGTATAATTAT+GGG - chr8_1:55171353-55171372 Msa1201890:exon 25.0%
! GCATCAAATTCATCATAATT+TGG + chr8_1:55171403-55171422 None:intergenic 25.0%
!!! TGTTATTTTCTTCATTCGTA+GGG + chr8_1:55172113-55172132 None:intergenic 25.0%
! GGTAATAAAGCAAAGTATAT+AGG + chr8_1:55171257-55171276 None:intergenic 25.0%
! TTGTGTAGGTTGTATAATTA+TGG - chr8_1:55171352-55171371 Msa1201890:exon 25.0%
! TGTGTAGGTTGTATAATTAT+GGG - chr8_1:55171353-55171372 Msa1201890:exon 25.0%
! GCATCAAATTCATCATAATT+TGG + chr8_1:55171403-55171422 None:intergenic 25.0%
!!! TGTTATTTTCTTCATTCGTA+GGG + chr8_1:55172113-55172132 None:intergenic 25.0%
! ATTTTCCAATATTCTCCCTT+AGG + chr8_1:55171062-55171081 None:intergenic 30.0%
TAAGGGAGAATATTGGAAAA+TGG - chr8_1:55171061-55171080 Msa1201890:three_prime_UTR 30.0%
AAGGGAGAATATTGGAAAAT+GGG - chr8_1:55171062-55171081 Msa1201890:three_prime_UTR 30.0%
CATTGAATTTGAAAGAGGAA+GGG + chr8_1:55171220-55171239 None:intergenic 30.0%
AGGTTGTATAATTATGGGTT+TGG - chr8_1:55171358-55171377 Msa1201890:CDS 30.0%
AATGATATATGGTTGTCCTT+GGG + chr8_1:55171454-55171473 None:intergenic 30.0%
! ACTAGAAATTTAGAATGCAC+AGG + chr8_1:55171489-55171508 None:intergenic 30.0%
AAAATGAGGTTGGAAGAAAT+GGG - chr8_1:55171589-55171608 Msa1201890:CDS 30.0%
CTTATCTTACAGACAAAAAG+AGG + chr8_1:55171913-55171932 None:intergenic 30.0%
! CTCTTTTTGTCTGTAAGATA+AGG - chr8_1:55171911-55171930 Msa1201890:intron 30.0%
! CACGATATACAACATAGTTT+TGG + chr8_1:55172006-55172025 None:intergenic 30.0%
!!! CTGTTATTTTCTTCATTCGT+AGG + chr8_1:55172114-55172133 None:intergenic 30.0%
! ATTTTCCAATATTCTCCCTT+AGG + chr8_1:55171062-55171081 None:intergenic 30.0%
TAAGGGAGAATATTGGAAAA+TGG - chr8_1:55171061-55171080 Msa1201890:three_prime_UTR 30.0%
AAGGGAGAATATTGGAAAAT+GGG - chr8_1:55171062-55171081 Msa1201890:three_prime_UTR 30.0%
CATTGAATTTGAAAGAGGAA+GGG + chr8_1:55171220-55171239 None:intergenic 30.0%
AGGTTGTATAATTATGGGTT+TGG - chr8_1:55171358-55171377 Msa1201890:CDS 30.0%
AATGATATATGGTTGTCCTT+GGG + chr8_1:55171454-55171473 None:intergenic 30.0%
! ACTAGAAATTTAGAATGCAC+AGG + chr8_1:55171489-55171508 None:intergenic 30.0%
AAAATGAGGTTGGAAGAAAT+GGG - chr8_1:55171589-55171608 Msa1201890:CDS 30.0%
CTTATCTTACAGACAAAAAG+AGG + chr8_1:55171913-55171932 None:intergenic 30.0%
! CTCTTTTTGTCTGTAAGATA+AGG - chr8_1:55171911-55171930 Msa1201890:intron 30.0%
! CACGATATACAACATAGTTT+TGG + chr8_1:55172006-55172025 None:intergenic 30.0%
!!! CTGTTATTTTCTTCATTCGT+AGG + chr8_1:55172114-55172133 None:intergenic 30.0%
AGGGAGAATATTGGAAAATG+GGG - chr8_1:55171063-55171082 Msa1201890:three_prime_UTR 35.0%
ATATTGGAAAATGGGGATTG+AGG - chr8_1:55171070-55171089 Msa1201890:three_prime_UTR 35.0%
AAAATGGGGATTGAGGAAAT+TGG - chr8_1:55171077-55171096 Msa1201890:three_prime_UTR 35.0%
CAACACTGATCTGCATAATA+TGG + chr8_1:55171147-55171166 None:intergenic 35.0%
CATATTATGCAGATCAGTGT+TGG - chr8_1:55171145-55171164 Msa1201890:three_prime_UTR 35.0%
AGGTTCTATGAAGAAGTCTT+GGG - chr8_1:55171176-55171195 Msa1201890:three_prime_UTR 35.0%
! TATGAAGAAGTCTTGGGTTT+TGG - chr8_1:55171182-55171201 Msa1201890:three_prime_UTR 35.0%
CCATTGAATTTGAAAGAGGA+AGG + chr8_1:55171221-55171240 None:intergenic 35.0%
CCTTCCTCTTTCAAATTCAA+TGG - chr8_1:55171218-55171237 Msa1201890:three_prime_UTR 35.0%
TGCTCCATTGAATTTGAAAG+AGG + chr8_1:55171225-55171244 None:intergenic 35.0%
CTTTCAAATTCAATGGAGCA+TGG - chr8_1:55171225-55171244 Msa1201890:three_prime_UTR 35.0%
GAATGATATATGGTTGTCCT+TGG + chr8_1:55171455-55171474 None:intergenic 35.0%
GAATGCACAGGTTAATTACT+TGG + chr8_1:55171477-55171496 None:intergenic 35.0%
GAAAATGAGGTTGGAAGAAA+TGG - chr8_1:55171588-55171607 Msa1201890:CDS 35.0%
AAATGAGGTTGGAAGAAATG+GGG - chr8_1:55171590-55171609 Msa1201890:CDS 35.0%
GATGAAGTATGTTACAGCTT+TGG - chr8_1:55171612-55171631 Msa1201890:CDS 35.0%
GTTCTATCATGTATCCATCA+GGG + chr8_1:55171681-55171700 None:intergenic 35.0%
AGTTCTATCATGTATCCATC+AGG + chr8_1:55171682-55171701 None:intergenic 35.0%
CAGAAGAAATGGGAACTATT+GGG + chr8_1:55171723-55171742 None:intergenic 35.0%
AAACAGTCTTCTTAAAGCTC+TGG + chr8_1:55171771-55171790 None:intergenic 35.0%
!! AAGACTGTTTCTAACAAGTG+TGG - chr8_1:55171781-55171800 Msa1201890:CDS 35.0%
!! AGACTGTTTCTAACAAGTGT+GGG - chr8_1:55171782-55171801 Msa1201890:CDS 35.0%
AGTGTTTGTTCTTCAAGACA+AGG - chr8_1:55171936-55171955 Msa1201890:intron 35.0%
TGTTCTTCAAGACAAGGAAA+AGG - chr8_1:55171942-55171961 Msa1201890:intron 35.0%
AGGGAGAATATTGGAAAATG+GGG - chr8_1:55171063-55171082 Msa1201890:three_prime_UTR 35.0%
ATATTGGAAAATGGGGATTG+AGG - chr8_1:55171070-55171089 Msa1201890:three_prime_UTR 35.0%
AAAATGGGGATTGAGGAAAT+TGG - chr8_1:55171077-55171096 Msa1201890:three_prime_UTR 35.0%
CAACACTGATCTGCATAATA+TGG + chr8_1:55171147-55171166 None:intergenic 35.0%
CATATTATGCAGATCAGTGT+TGG - chr8_1:55171145-55171164 Msa1201890:three_prime_UTR 35.0%
AGGTTCTATGAAGAAGTCTT+GGG - chr8_1:55171176-55171195 Msa1201890:three_prime_UTR 35.0%
! TATGAAGAAGTCTTGGGTTT+TGG - chr8_1:55171182-55171201 Msa1201890:three_prime_UTR 35.0%
CCATTGAATTTGAAAGAGGA+AGG + chr8_1:55171221-55171240 None:intergenic 35.0%
CCTTCCTCTTTCAAATTCAA+TGG - chr8_1:55171218-55171237 Msa1201890:three_prime_UTR 35.0%
TGCTCCATTGAATTTGAAAG+AGG + chr8_1:55171225-55171244 None:intergenic 35.0%
CTTTCAAATTCAATGGAGCA+TGG - chr8_1:55171225-55171244 Msa1201890:three_prime_UTR 35.0%
GAATGATATATGGTTGTCCT+TGG + chr8_1:55171455-55171474 None:intergenic 35.0%
GAATGCACAGGTTAATTACT+TGG + chr8_1:55171477-55171496 None:intergenic 35.0%
GAAAATGAGGTTGGAAGAAA+TGG - chr8_1:55171588-55171607 Msa1201890:CDS 35.0%
AAATGAGGTTGGAAGAAATG+GGG - chr8_1:55171590-55171609 Msa1201890:CDS 35.0%
GATGAAGTATGTTACAGCTT+TGG - chr8_1:55171612-55171631 Msa1201890:CDS 35.0%
GTTCTATCATGTATCCATCA+GGG + chr8_1:55171681-55171700 None:intergenic 35.0%
AGTTCTATCATGTATCCATC+AGG + chr8_1:55171682-55171701 None:intergenic 35.0%
CAGAAGAAATGGGAACTATT+GGG + chr8_1:55171723-55171742 None:intergenic 35.0%
AAACAGTCTTCTTAAAGCTC+TGG + chr8_1:55171771-55171790 None:intergenic 35.0%
!! AAGACTGTTTCTAACAAGTG+TGG - chr8_1:55171781-55171800 Msa1201890:CDS 35.0%
!! AGACTGTTTCTAACAAGTGT+GGG - chr8_1:55171782-55171801 Msa1201890:CDS 35.0%
AGTGTTTGTTCTTCAAGACA+AGG - chr8_1:55171936-55171955 Msa1201890:intron 35.0%
TGTTCTTCAAGACAAGGAAA+AGG - chr8_1:55171942-55171961 Msa1201890:intron 35.0%
!! TGAGTGAAGAAGTGTCCTAA+GGG - chr8_1:55171044-55171063 Msa1201890:three_prime_UTR 40.0%
AGTGTCCTAAGGGAGAATAT+TGG - chr8_1:55171054-55171073 Msa1201890:three_prime_UTR 40.0%
ACATGATTCAGTGAGAGAAG+GGG + chr8_1:55171122-55171141 None:intergenic 40.0%
CACATGATTCAGTGAGAGAA+GGG + chr8_1:55171123-55171142 None:intergenic 40.0%
ACACATGATTCAGTGAGAGA+AGG + chr8_1:55171124-55171143 None:intergenic 40.0%
GAGGTTCTATGAAGAAGTCT+TGG - chr8_1:55171175-55171194 Msa1201890:three_prime_UTR 40.0%
TGGTTGTCCTTGGGATTAAT+GGG + chr8_1:55171445-55171464 None:intergenic 40.0%
ATGGTTGTCCTTGGGATTAA+TGG + chr8_1:55171446-55171465 None:intergenic 40.0%
TTTGTGCAGTGTACAGATGT+TGG - chr8_1:55171559-55171578 Msa1201890:CDS 40.0%
TTGTGCAGTGTACAGATGTT+GGG - chr8_1:55171560-55171579 Msa1201890:CDS 40.0%
!! ATGTTGGGCTAGTGAAAATG+AGG - chr8_1:55171575-55171594 Msa1201890:CDS 40.0%
! GTATGTTACAGCTTTGGTAG+AGG - chr8_1:55171618-55171637 Msa1201890:CDS 40.0%
GCAGAAGAAATGGGAACTAT+TGG + chr8_1:55171724-55171743 None:intergenic 40.0%
TTCTAACAAGTGTGGGTTCA+TGG - chr8_1:55171789-55171808 Msa1201890:CDS 40.0%
! TACGTATTAGCTTGGAAGTG+TGG + chr8_1:55171009-55171028 None:intergenic 40.0%
!! TGAGTGAAGAAGTGTCCTAA+GGG - chr8_1:55171044-55171063 Msa1201890:three_prime_UTR 40.0%
AGTGTCCTAAGGGAGAATAT+TGG - chr8_1:55171054-55171073 Msa1201890:three_prime_UTR 40.0%
ACATGATTCAGTGAGAGAAG+GGG + chr8_1:55171122-55171141 None:intergenic 40.0%
CACATGATTCAGTGAGAGAA+GGG + chr8_1:55171123-55171142 None:intergenic 40.0%
ACACATGATTCAGTGAGAGA+AGG + chr8_1:55171124-55171143 None:intergenic 40.0%
GAGGTTCTATGAAGAAGTCT+TGG - chr8_1:55171175-55171194 Msa1201890:three_prime_UTR 40.0%
TGGTTGTCCTTGGGATTAAT+GGG + chr8_1:55171445-55171464 None:intergenic 40.0%
ATGGTTGTCCTTGGGATTAA+TGG + chr8_1:55171446-55171465 None:intergenic 40.0%
TTTGTGCAGTGTACAGATGT+TGG - chr8_1:55171559-55171578 Msa1201890:CDS 40.0%
TTGTGCAGTGTACAGATGTT+GGG - chr8_1:55171560-55171579 Msa1201890:CDS 40.0%
!! ATGTTGGGCTAGTGAAAATG+AGG - chr8_1:55171575-55171594 Msa1201890:CDS 40.0%
! GTATGTTACAGCTTTGGTAG+AGG - chr8_1:55171618-55171637 Msa1201890:CDS 40.0%
GCAGAAGAAATGGGAACTAT+TGG + chr8_1:55171724-55171743 None:intergenic 40.0%
TTCTAACAAGTGTGGGTTCA+TGG - chr8_1:55171789-55171808 Msa1201890:CDS 40.0%
!! CTGAGTGAAGAAGTGTCCTA+AGG - chr8_1:55171043-55171062 Msa1201890:three_prime_UTR 45.0%
! GATCAGTGTTGGACTCTATG+AGG - chr8_1:55171156-55171175 Msa1201890:three_prime_UTR 45.0%
TGGGTCGTGATTCACTCATA+GGG + chr8_1:55171426-55171445 None:intergenic 45.0%
ATGGGTCGTGATTCACTCAT+AGG + chr8_1:55171427-55171446 None:intergenic 45.0%
ATCACGACCCATTAATCCCA+AGG - chr8_1:55171435-55171454 Msa1201890:CDS 45.0%
!! TGGGCTAGTGAAAATGAGGT+TGG - chr8_1:55171579-55171598 Msa1201890:CDS 45.0%
! ACAGCTTTGGTAGAGGATGA+AGG - chr8_1:55171625-55171644 Msa1201890:CDS 45.0%
GGTAGAGGATGAAGGAATCA+AGG - chr8_1:55171633-55171652 Msa1201890:CDS 45.0%
AGAGGATGAAGGAATCAAGG+TGG - chr8_1:55171636-55171655 Msa1201890:intron 45.0%
TCATGTATCCATCAGGGTCA+TGG + chr8_1:55171675-55171694 None:intergenic 45.0%
GAGCAGGAAGCAGAAGAAAT+GGG + chr8_1:55171733-55171752 None:intergenic 45.0%
AGAGCAGGAAGCAGAAGAAA+TGG + chr8_1:55171734-55171753 None:intergenic 45.0%
TTCTGCTTCCTGCTCTTTCA+AGG - chr8_1:55171738-55171757 Msa1201890:intron 45.0%
TATGATGCGAAGCTTGAGCA+TGG - chr8_1:55171819-55171838 Msa1201890:CDS 45.0%
!! CTGAGTGAAGAAGTGTCCTA+AGG - chr8_1:55171043-55171062 Msa1201890:three_prime_UTR 45.0%
! GATCAGTGTTGGACTCTATG+AGG - chr8_1:55171156-55171175 Msa1201890:three_prime_UTR 45.0%
TGGGTCGTGATTCACTCATA+GGG + chr8_1:55171426-55171445 None:intergenic 45.0%
ATGGGTCGTGATTCACTCAT+AGG + chr8_1:55171427-55171446 None:intergenic 45.0%
ATCACGACCCATTAATCCCA+AGG - chr8_1:55171435-55171454 Msa1201890:CDS 45.0%
!! TGGGCTAGTGAAAATGAGGT+TGG - chr8_1:55171579-55171598 Msa1201890:CDS 45.0%
! ACAGCTTTGGTAGAGGATGA+AGG - chr8_1:55171625-55171644 Msa1201890:CDS 45.0%
GGTAGAGGATGAAGGAATCA+AGG - chr8_1:55171633-55171652 Msa1201890:CDS 45.0%
AGAGGATGAAGGAATCAAGG+TGG - chr8_1:55171636-55171655 Msa1201890:intron 45.0%
TCATGTATCCATCAGGGTCA+TGG + chr8_1:55171675-55171694 None:intergenic 45.0%
GAGCAGGAAGCAGAAGAAAT+GGG + chr8_1:55171733-55171752 None:intergenic 45.0%
AGAGCAGGAAGCAGAAGAAA+TGG + chr8_1:55171734-55171753 None:intergenic 45.0%
TTCTGCTTCCTGCTCTTTCA+AGG - chr8_1:55171738-55171757 Msa1201890:intron 45.0%
TATGATGCGAAGCTTGAGCA+TGG - chr8_1:55171819-55171838 Msa1201890:CDS 45.0%
TTCAGTGAGAGAAGGGGTAG+TGG + chr8_1:55171116-55171135 None:intergenic 50.0%
GGGTCGTGATTCACTCATAG+GGG + chr8_1:55171425-55171444 None:intergenic 50.0%
TGAAGGAATCAAGGTGGAGC+AGG - chr8_1:55171642-55171661 Msa1201890:intron 50.0%
GTGTTCTTCCATGACCCTGA+TGG - chr8_1:55171664-55171683 Msa1201890:intron 50.0%
TTCAGTGAGAGAAGGGGTAG+TGG + chr8_1:55171116-55171135 None:intergenic 50.0%
GGGTCGTGATTCACTCATAG+GGG + chr8_1:55171425-55171444 None:intergenic 50.0%
TGAAGGAATCAAGGTGGAGC+AGG - chr8_1:55171642-55171661 Msa1201890:intron 50.0%
GTGTTCTTCCATGACCCTGA+TGG - chr8_1:55171664-55171683 Msa1201890:intron 50.0%
TCCTGCTCTTTCAAGGCACG+AGG - chr8_1:55171745-55171764 Msa1201890:CDS 55.0%
TCCTGCTCTTTCAAGGCACG+AGG - chr8_1:55171745-55171764 Msa1201890:CDS 55.0%
GCCTCGTGCCTTGAAAGAGC+AGG + chr8_1:55171749-55171768 None:intergenic 60.0%
GCCTCGTGCCTTGAAAGAGC+AGG + chr8_1:55171749-55171768 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 55171009 55172213 55171009 ID=Msa1201890;Name=Msa1201890
chr8_1 mRNA 55171009 55172213 55171009 ID=Msa1201890-mRNA-1;Parent=Msa1201890;Name=Msa1201890-mRNA-1;_AED=0.08;_eAED=0.08;_QI=71|1|1|1|1|1|3|349|193
chr8_1 exon 55171009 55171654 55171009 ID=Msa1201890-mRNA-1:exon:17841;Parent=Msa1201890-mRNA-1
chr8_1 exon 55171745 55171862 55171745 ID=Msa1201890-mRNA-1:exon:17840;Parent=Msa1201890-mRNA-1
chr8_1 exon 55171976 55172213 55171976 ID=Msa1201890-mRNA-1:exon:17839;Parent=Msa1201890-mRNA-1
chr8_1 five_prime_UTR 55172143 55172213 55172143 ID=Msa1201890-mRNA-1:five_prime_utr;Parent=Msa1201890-mRNA-1
chr8_1 CDS 55171976 55172142 55171976 ID=Msa1201890-mRNA-1:cds;Parent=Msa1201890-mRNA-1
chr8_1 CDS 55171745 55171862 55171745 ID=Msa1201890-mRNA-1:cds;Parent=Msa1201890-mRNA-1
chr8_1 CDS 55171358 55171654 55171358 ID=Msa1201890-mRNA-1:cds;Parent=Msa1201890-mRNA-1
chr8_1 three_prime_UTR 55171009 55171357 55171009 ID=Msa1201890-mRNA-1:three_prime_utr;Parent=Msa1201890-mRNA-1
Gene Sequence

>Msa1201890

ATGGGGATTGAGGAAATTGGCAGCTATGAAGCACCACTACCCCTTCTCTCACTGAATCATGTGTCCATATTATGCAGATCAGTGTTGGACTCTATGAGGTTCTATGAAGAAGTCTTGGGTTTTGGTCTCATCAAACGCCCTTCCTCTTTCAAATTCAATGGAGCATGGTTGTATAATTATGGGTTTGGCATACACTTGCTTGAGAATCCAAATTATGATGAATTTGATGCCCCTATGAGTGAATCACGACCCATTAATCCCAAGGACAACCATATATCATTCCAATGTACAGATGTTGGGCTAGTGAAAATGAGGTTGGAAGAAATGGGGATGAAGTATGTTACAGCTTTGGTAGAGGATGAAGGAATCAAGGTGGAGCAGGTGTTCTTCCATGACCCTGATGGATACATGATAGAACTTTGCAACTGTGAGAATATCCCAATAGTTCCCATTTCTTCTGCTTCCTGCTCTTTCAAGGCACGAGGCCAGAGCTTTAAGAAGACTGTTTCTAACAAGTGTGGGTTCATGGAAAATGTTATGATGCGAAGCTTGAGCATGGACATGATGAACTTTGCGTTTTAA
Protein sequence

>Msa1201890

MGIEEIGSYEAPLPLLSLNHVSILCRSVLDSMRFYEEVLGFGLIKRPSSFKFNGAWLYNYGFGIHLLENPNYDEFDAPMSESRPINPKDNHISFQCTDVGLVKMRLEEMGMKYVTALVEDEGIKVEQVFFHDPDGYMIELCNCENIPIVPISSASCSFKARGQSFKKTVSNKCGFMENVMMRSLSMDMMNFAF*