AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1449730


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 11 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAAATTCACATAATGAT+TGG 0.393884 tig0032678:+27089 None:intergenic
TGGTTTGGTGCAGGTTATGA+TGG 0.410190 tig0032678:-29719 None:intergenic
TTTCCCATGATGGGAAAAGC+TGG 0.461719 tig0032678:-29682 Msa1449730:CDS
AGAAGATTATGCTTCTCAAC+GGG 0.498617 tig0032678:-27168 Msa1449730:CDS
ATGGGAAAAGCTGGGTTGAC+TGG 0.514865 tig0032678:-29673 Msa1449730:intron
TTCCCATGATGGGAAAAGCT+GGG 0.535647 tig0032678:-29681 Msa1449730:CDS
GGTTTGGTGCAGGTTATGAT+GGG 0.539945 tig0032678:-29718 None:intergenic
TAGAAGATTATGCTTCTCAA+CGG 0.625011 tig0032678:-27169 Msa1449730:CDS
TGGAACATCGAGGTGGCTGA+AGG 0.628575 tig0032678:+27109 Msa1449720:intergenic
TAATGATTGGAACATCGAGG+TGG 0.726775 tig0032678:+27102 Msa1449720:intergenic
ACATAATGATTGGAACATCG+AGG 0.733437 tig0032678:+27099 Msa1449720:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATATTCATAAAATAG+GGG + tig0032678:27261-27280 Msa1449730:intergenic 10.0%
!! GTTAATAATATTCATAAAAT+AGG + tig0032678:27263-27282 Msa1449730:intergenic 10.0%
!! TTGATATATTCATAAAATAT+TGG + tig0032678:28081-28100 Msa1449730:intergenic 10.0%
!!! ATATTTTATGAATATATCAA+TGG - tig0032678:28081-28100 Msa1449730:intron 10.0%
!! AATATAATAAAGACATAAGT+GGG + tig0032678:27227-27246 Msa1449730:intergenic 15.0%
!! AAATATAATAAAGACATAAG+TGG + tig0032678:27228-27247 Msa1449730:intergenic 15.0%
!! AATCAAAGAATCTATTAATT+CGG - tig0032678:27410-27429 Msa1449730:intron 15.0%
!! TAAAATTACTATAAGGTTAT+TGG - tig0032678:27652-27671 Msa1449730:intron 15.0%
!! TTATAAATGTTGATCAATAT+AGG + tig0032678:27969-27988 Msa1449730:intergenic 15.0%
!! TATTGATCAACATTTATAAT+TGG - tig0032678:27970-27989 Msa1449730:intron 15.0%
!! TAAATTTACTAAAACTAACA+AGG - tig0032678:28015-28034 Msa1449730:intron 15.0%
!! TATATTCATAAAATATTGGA+TGG + tig0032678:28077-28096 Msa1449730:intergenic 15.0%
!!! TTTTTATGCTTTTGAATATA+AGG - tig0032678:28476-28495 Msa1449730:intron 15.0%
!!! TAATAATTTTATCCCATTTA+CGG - tig0032678:28570-28589 Msa1449730:intron 15.0%
!! ACTAGATAATATCTATTAAT+TGG - tig0032678:28598-28617 Msa1449730:intron 15.0%
!! AAAAATGAAATTGACAATTT+CGG - tig0032678:28851-28870 Msa1449730:intron 15.0%
!!! ACAATCATATTTAGTTTTGT+GGG + tig0032678:27200-27219 Msa1449730:intergenic 20.0%
!!! TACAATCATATTTAGTTTTG+TGG + tig0032678:27201-27220 Msa1449730:intergenic 20.0%
!! GGAATAATTTATTATTCTCT+CGG + tig0032678:27337-27356 Msa1449730:intergenic 20.0%
!! TGTTCGATAAAATTACTATA+AGG - tig0032678:27645-27664 Msa1449730:intron 20.0%
!! ACATGTAAAAAATGTAATTC+TGG + tig0032678:27688-27707 Msa1449730:intergenic 20.0%
!!! ATTTCATTTTTAATTTGCTG+CGG + tig0032678:27910-27929 Msa1449730:intergenic 20.0%
!! CATTTATAATTGGACAAAAA+TGG - tig0032678:27980-27999 Msa1449730:intron 20.0%
!! TATTCTACTAATTTGATATC+AGG + tig0032678:28212-28231 Msa1449730:intergenic 20.0%
!! CATTGAATAATTGATATATC+TGG + tig0032678:28351-28370 Msa1449730:intergenic 20.0%
!! TATCAATAAATACTTTCTCT+AGG + tig0032678:28410-28429 Msa1449730:intergenic 20.0%
!!! TCATTGTATTATTTGCTATT+TGG - tig0032678:28943-28962 Msa1449730:intron 20.0%
!!! AAATTTCTAGTAAATCGAAA+TGG + tig0032678:29401-29420 Msa1449730:intergenic 20.0%
!! TTTGATGTGAATAATAATGT+GGG + tig0032678:29515-29534 Msa1449730:intergenic 20.0%
!! ATTTGATGTGAATAATAATG+TGG + tig0032678:29516-29535 Msa1449730:intergenic 20.0%
!!! AATCATATTTAGTTTTGTGG+GGG + tig0032678:27198-27217 Msa1449730:intergenic 25.0%
!!! CAATCATATTTAGTTTTGTG+GGG + tig0032678:27199-27218 Msa1449730:intergenic 25.0%
! CTAATTCAGAGTATATTAGA+AGG - tig0032678:27437-27456 Msa1449730:intron 25.0%
! TTGTACAAAATATGCAGAAT+TGG + tig0032678:27554-27573 Msa1449730:intergenic 25.0%
! TTATAATTGGACAAAAATGG+AGG - tig0032678:27983-28002 Msa1449730:intron 25.0%
! TATAATTGGACAAAAATGGA+GGG - tig0032678:27984-28003 Msa1449730:intron 25.0%
!!! ACTTGTTAATTTTCCATGAT+GGG + tig0032678:28122-28141 Msa1449730:intergenic 25.0%
!! AAAAGGTGACTTTTTATGAT+GGG + tig0032678:28148-28167 Msa1449730:intergenic 25.0%
!! TATTATTTTCTGACAGCAAA+TGG + tig0032678:28555-28574 Msa1449730:intergenic 25.0%
!!! TGTATTATTTGCTATTTGGA+TGG - tig0032678:28947-28966 Msa1449730:intron 25.0%
! AAAAATGATATTGATGAGTG+TGG - tig0032678:29208-29227 Msa1449730:intron 25.0%
! AAAATGATATTGATGAGTGT+GGG - tig0032678:29209-29228 Msa1449730:intron 25.0%
! AAAACAGAAATGAAATCACT+TGG + tig0032678:29468-29487 Msa1449730:intergenic 25.0%
! ACTTCATAATTTTCCCATGA+TGG - tig0032678:27111-27130 Msa1449730:CDS 30.0%
! CTTCATAATTTTCCCATGAT+GGG - tig0032678:27112-27131 Msa1449730:CDS 30.0%
ATAACAATACAGTGATTGTG+GGG + tig0032678:27358-27377 Msa1449730:intergenic 30.0%
GATAACAATACAGTGATTGT+GGG + tig0032678:27359-27378 Msa1449730:intergenic 30.0%
TGATAACAATACAGTGATTG+TGG + tig0032678:27360-27379 Msa1449730:intergenic 30.0%
ACTAAAACTAACAAGGCAAT+TGG - tig0032678:28022-28041 Msa1449730:intron 30.0%
! TATTTGACATTTTCCCATCA+TGG - tig0032678:28106-28125 Msa1449730:intron 30.0%
!!! GACTTGTTAATTTTCCATGA+TGG + tig0032678:28123-28142 Msa1449730:intergenic 30.0%
! CAAAAGGTGACTTTTTATGA+TGG + tig0032678:28149-28168 Msa1449730:intergenic 30.0%
! CATCATAAAAAGTCACCTTT+TGG - tig0032678:28147-28166 Msa1449730:intron 30.0%
!!! AAAGTCACCTTTTGGTTTTA+CGG - tig0032678:28155-28174 Msa1449730:intron 30.0%
!!! ATCAGGTGTATCTTTTTTAG+AGG + tig0032678:28195-28214 Msa1449730:intergenic 30.0%
ATCTAGTGTTATCCGTAAAT+GGG + tig0032678:28585-28604 Msa1449730:intergenic 30.0%
TATCTAGTGTTATCCGTAAA+TGG + tig0032678:28586-28605 Msa1449730:intergenic 30.0%
AATGAAATTGACAATTTCGG+CGG - tig0032678:28854-28873 Msa1449730:intron 30.0%
AATTACATCGTTCATTCAAG+GGG + tig0032678:28920-28939 Msa1449730:intergenic 30.0%
CAATTACATCGTTCATTCAA+GGG + tig0032678:28921-28940 Msa1449730:intergenic 30.0%
TCAATTACATCGTTCATTCA+AGG + tig0032678:28922-28941 Msa1449730:intergenic 30.0%
TCAAATAGAAGAAACTGAGT+AGG + tig0032678:28997-29016 Msa1449730:intergenic 30.0%
!! TTATTTCTAAATGACATCCG+AGG - tig0032678:29107-29126 Msa1449730:intron 30.0%
ATGATCATCCTTACTACTTT+GGG - tig0032678:29130-29149 Msa1449730:intron 30.0%
ATATCTCATCTAAAGGAGTA+AGG + tig0032678:29179-29198 Msa1449730:intergenic 30.0%
CAAACTGATATCTCATCTAA+AGG + tig0032678:29186-29205 Msa1449730:intergenic 30.0%
AAATGATATTGATGAGTGTG+GGG - tig0032678:29210-29229 Msa1449730:intron 30.0%
AAGACTTGGAGTAAAGATAA+CGG - tig0032678:29259-29278 Msa1449730:intron 30.0%
!!! TTGCTTTTTCTTTCCATCTT+TGG - tig0032678:29312-29331 Msa1449730:intron 30.0%
TGGATAAGTTAGTACTTAGT+AGG + tig0032678:29448-29467 Msa1449730:intergenic 30.0%
TGGGAATGAAATGCTTTATA+TGG + tig0032678:29496-29515 Msa1449730:intergenic 30.0%
! AACAAGAAGTATGACGTTAT+TGG + tig0032678:29567-29586 Msa1449730:intergenic 30.0%
TAGAAGATTATGCTTCTCAA+CGG - tig0032678:29634-29653 Msa1449730:intron 30.0%
! AACGTCATTTGAGTTATGCA+TGG + tig0032678:27295-27314 Msa1449730:intergenic 35.0%
AATGACGTTCTTATAGTGAC+CGG - tig0032678:27306-27325 Msa1449730:intron 35.0%
ATATACCTGTCGTAACAAAC+AGG - tig0032678:27763-27782 Msa1449730:intron 35.0%
ATGATTGAGCTTAGCAGATT+TGG + tig0032678:27789-27808 Msa1449730:intergenic 35.0%
AAAAAAAGAGACAGTCCATC+AGG - tig0032678:27940-27959 Msa1449730:intron 35.0%
AAAAAAGAGACAGTCCATCA+GGG - tig0032678:27941-27960 Msa1449730:intron 35.0%
AACAAGGCAATTGGAACAAA+AGG - tig0032678:28031-28050 Msa1449730:intron 35.0%
AGTTGTACCGTAAAACCAAA+AGG + tig0032678:28165-28184 Msa1449730:intergenic 35.0%
GAAACTGAGTAGGTCATAAA+TGG + tig0032678:28987-29006 Msa1449730:intergenic 35.0%
GATGATCATCCTTACTACTT+TGG - tig0032678:29129-29148 Msa1449730:intron 35.0%
TTGAAAGACCCAAAGTAGTA+AGG + tig0032678:29141-29160 Msa1449730:intergenic 35.0%
CAGTTTCACGTTATAAGACT+TGG - tig0032678:29245-29264 Msa1449730:intron 35.0%
AAAAGCAATGCTGGTACTTT+GGG + tig0032678:29300-29319 Msa1449730:intergenic 35.0%
AAAAAGCAATGCTGGTACTT+TGG + tig0032678:29301-29320 Msa1449730:intergenic 35.0%
ATGGAAAGAAAAAGCAATGC+TGG + tig0032678:29309-29328 Msa1449730:intergenic 35.0%
AAGGAAGAAAAAGCCAAAGA+TGG + tig0032678:29328-29347 Msa1449730:intergenic 35.0%
! TTCCTTTTTGACCAGTTTCT+GGG - tig0032678:29342-29361 Msa1449730:intron 35.0%
AGTTAGTACTTAGTAGGTAC+TGG + tig0032678:29442-29461 Msa1449730:intergenic 35.0%
! GCTAAAGTTTTCAGACAACA+TGG - tig0032678:29539-29558 Msa1449730:intron 35.0%
AGAAGATTATGCTTCTCAAC+GGG - tig0032678:29635-29654 Msa1449730:intron 35.0%
ACATAATGATTGGAACATCG+AGG + tig0032678:29707-29726 Msa1449730:intergenic 35.0%
GGGGGTGAAAACAGTAATAA+AGG + tig0032678:27180-27199 Msa1449730:intergenic 40.0%
TATTATTCTCTCGGTCTCAC+CGG + tig0032678:27328-27347 Msa1449730:intergenic 40.0%
GATCAATATAGGATCCCTGA+TGG + tig0032678:27958-27977 Msa1449730:intergenic 40.0%
AGGTACCCAATCTCATATCT+AGG - tig0032678:28051-28070 Msa1449730:intron 40.0%
GATGGCCTAGATATGAGATT+GGG + tig0032678:28059-28078 Msa1449730:intergenic 40.0%
TAATCGTGTGACTCACTTAG+TGG - tig0032678:29063-29082 Msa1449730:intron 40.0%
GTAGTAAGGATGATCATCCT+CGG + tig0032678:29127-29146 Msa1449730:intergenic 40.0%
!! GTACTTTGGGACTGAATTTC+TGG + tig0032678:29287-29306 Msa1449730:intergenic 40.0%
! CTTCCTTTTTGACCAGTTTC+TGG - tig0032678:29341-29360 Msa1449730:intron 40.0%
ACTGGACAAGAGGACAATAA+AGG + tig0032678:29424-29443 Msa1449730:intergenic 40.0%
! CTCAGTCTTCTACGTTTTCT+GGG - tig0032678:29596-29615 Msa1449730:intron 40.0%
TAATGATTGGAACATCGAGG+TGG + tig0032678:29704-29723 Msa1449730:intergenic 40.0%
! TTTCCCATGATGGGAAAAGC+TGG - tig0032678:27121-27140 Msa1449730:CDS 45.0%
! TTCCCATGATGGGAAAAGCT+GGG - tig0032678:27122-27141 Msa1449730:CDS 45.0%
! AACCCAGCTTTTCCCATCAT+GGG + tig0032678:27127-27146 Msa1449730:intergenic 45.0%
! TTTGGCCTGTTTGTTACGAC+AGG + tig0032678:27771-27790 Msa1449730:intergenic 45.0%
GGATGGCCTAGATATGAGAT+TGG + tig0032678:28060-28079 Msa1449730:intergenic 45.0%
!! TTCGGCGGACTACATTCATA+TGG - tig0032678:28869-28888 Msa1449730:intron 45.0%
CACCCAGAAACTGGTCAAAA+AGG + tig0032678:29347-29366 Msa1449730:intergenic 45.0%
TTTCTGGGTGTCTTGGCTAA+GGG - tig0032678:29357-29376 Msa1449730:intron 45.0%
!! CTTAGTAGGTACTGGACAAG+AGG + tig0032678:29434-29453 Msa1449730:intergenic 45.0%
CCAGAAAACGTAGAAGACTG+AGG + tig0032678:29598-29617 Msa1449730:intergenic 45.0%
! CCTCAGTCTTCTACGTTTTC+TGG - tig0032678:29595-29614 Msa1449730:intron 45.0%
!! TTAATAATATTCATAAAATA+GGG + tig0032678:27262-27281 Msa1449730:intergenic 5.0%
!!! AATTTTTTTACTTTTTTTTT+AGG - tig0032678:27858-27877 Msa1449730:intron 5.0%
! CAACCCAGCTTTTCCCATCA+TGG + tig0032678:27128-27147 Msa1449730:intergenic 50.0%
! ATGGGAAAAGCTGGGTTGAC+TGG - tig0032678:27130-27149 Msa1449730:CDS 50.0%
ATTGATGAGTGTGGGGCACA+TGG - tig0032678:29217-29236 Msa1449730:intron 50.0%
! TGACCAGTTTCTGGGTGTCT+TGG - tig0032678:29350-29369 Msa1449730:intron 50.0%
TAGCCAAGACACCCAGAAAC+TGG + tig0032678:29356-29375 Msa1449730:intergenic 50.0%
! GTTTCTGGGTGTCTTGGCTA+AGG - tig0032678:29356-29375 Msa1449730:intron 50.0%
TGGAACATCGAGGTGGCTGA+AGG + tig0032678:29697-29716 Msa1449730:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0032678 gene 27097 29728 27097 ID=Msa1449730;Name=Msa1449730
tig0032678 mRNA 27097 29728 27097 ID=Msa1449730-mRNA-1;Parent=Msa1449730;Name=Msa1449730-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|0|0|0.5|1|1|2|0|60
tig0032678 exon 29674 29728 29674 ID=Msa1449730-mRNA-1:exon:17099;Parent=Msa1449730-mRNA-1
tig0032678 exon 27097 27224 27097 ID=Msa1449730-mRNA-1:exon:17098;Parent=Msa1449730-mRNA-1
tig0032678 CDS 29674 29728 29674 ID=Msa1449730-mRNA-1:cds;Parent=Msa1449730-mRNA-1
tig0032678 CDS 27097 27224 27097 ID=Msa1449730-mRNA-1:cds;Parent=Msa1449730-mRNA-1
Gene Sequence

>Msa1449730

GTTATGATGGGAAGACTTCATAATTTTCCCATGATGGGAAAAGCTGGGTTGACTGTCTTCTACGTTTTCTGGGATGCTCGAATTTTGCTAGAAGATTATGCTTCTCAACGGGATCGTCTTCATTTTCAATCGTCTTCATTCAGTTACACCTTCAGCCACCTCGATGTTCCAATCATTATGTGA

Protein sequence

>Msa1449730

VMMGRLHNFPMMGKAGLTVFYVFWDARILLEDYASQRDRLHFQSSSFSYTFSHLDVPIIM*