Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1205960 | XP_024636505.1 | 92.000 | 150 | 11 | 1 | 1 | 149 | 1 | 150 | 1.23e-93 | 277 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1205960 | G7JF71 | 92.000 | 150 | 11 | 1 | 1 | 149 | 1 | 150 | 5.87e-94 | 277 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa1205960 | 0.833266 | 5.884784e-56 | -8.615850e-47 |
| Msa0025330 | Msa1205960 | 0.822947 | 1.802936e-53 | -8.615850e-47 |
| Msa0028640 | Msa1205960 | 0.802890 | 4.526533e-49 | -8.615850e-47 |
| Msa0036960 | Msa1205960 | 0.809447 | 1.883797e-50 | -8.615850e-47 |
| Msa0052730 | Msa1205960 | 0.807613 | 4.641405e-50 | -8.615850e-47 |
| Msa0072370 | Msa1205960 | 0.801032 | 1.090155e-48 | -8.615850e-47 |
| Msa0072900 | Msa1205960 | 0.803878 | 2.825278e-49 | -8.615850e-47 |
| Msa0609430 | Msa1205960 | 0.828947 | 6.773011e-55 | -8.615850e-47 |
| Msa0611380 | Msa1205960 | 0.818458 | 1.939582e-52 | -8.615850e-47 |
| Msa0631120 | Msa1205960 | 0.812578 | 3.951436e-51 | -8.615850e-47 |
| Msa0647980 | Msa1205960 | 0.804712 | 1.893353e-49 | -8.615850e-47 |
| Msa0650070 | Msa1205960 | 0.814356 | 1.606611e-51 | -8.615850e-47 |
| Msa0662500 | Msa1205960 | 0.811758 | 5.965322e-51 | -8.615850e-47 |
| Msa0669340 | Msa1205960 | 0.805314 | 1.416890e-49 | -8.615850e-47 |
| Msa0684500 | Msa1205960 | 0.827867 | 1.234073e-54 | -8.615850e-47 |
| Msa0688480 | Msa1205960 | 0.803459 | 3.450759e-49 | -8.615850e-47 |
| Msa0692060 | Msa1205960 | 0.826765 | 2.266350e-54 | -8.615850e-47 |
| Msa0716450 | Msa1205960 | 0.800839 | 1.193838e-48 | -8.615850e-47 |
| Msa0727710 | Msa1205960 | 0.814841 | 1.254558e-51 | -8.615850e-47 |
| Msa0745790 | Msa1205960 | 0.814170 | 1.765680e-51 | -8.615850e-47 |
| Msa0758320 | Msa1205960 | 0.805092 | 1.577102e-49 | -8.615850e-47 |
| Msa0796530 | Msa1205960 | 0.801948 | 7.075620e-49 | -8.615850e-47 |
| Msa0837980 | Msa1205960 | 0.802450 | 5.577785e-49 | -8.615850e-47 |
| Msa0121090 | Msa1205960 | 0.804881 | 1.746033e-49 | -8.615850e-47 |
| Msa0141100 | Msa1205960 | 0.829694 | 4.460625e-55 | -8.615850e-47 |
| Msa0157290 | Msa1205960 | 0.833283 | 5.830780e-56 | -8.615850e-47 |
| Msa0160130 | Msa1205960 | 0.816535 | 5.259981e-52 | -8.615850e-47 |
| Msa0163980 | Msa1205960 | 0.818476 | 1.921281e-52 | -8.615850e-47 |
| Msa0164980 | Msa1205960 | 0.814704 | 1.345080e-51 | -8.615850e-47 |
| Msa0174320 | Msa1205960 | 0.809623 | 1.726826e-50 | -8.615850e-47 |
| Msa0223690 | Msa1205960 | 0.806655 | 7.402981e-50 | -8.615850e-47 |
| Msa0225600 | Msa1205960 | 0.803404 | 3.542683e-49 | -8.615850e-47 |
| Msa0366240 | Msa1205960 | 0.803063 | 4.168782e-49 | -8.615850e-47 |
| Msa0374850 | Msa1205960 | 0.826558 | 2.539451e-54 | -8.615850e-47 |
| Msa0375090 | Msa1205960 | 0.816806 | 4.573594e-52 | -8.615850e-47 |
| Msa0470340 | Msa1205960 | 0.811064 | 8.437762e-51 | -8.615850e-47 |
| Msa1013400 | Msa1205960 | 0.821069 | 4.910721e-53 | -8.615850e-47 |
| Msa1024740 | Msa1205960 | 0.816887 | 4.385064e-52 | -8.615850e-47 |
| Msa1082500 | Msa1205960 | 0.802565 | 5.282731e-49 | -8.615850e-47 |
| Msa1093530 | Msa1205960 | 0.811113 | 8.236660e-51 | -8.615850e-47 |
| Msa0266450 | Msa1205960 | 0.818191 | 2.229175e-52 | -8.615850e-47 |
| Msa0279790 | Msa1205960 | 0.803890 | 2.808901e-49 | -8.615850e-47 |
| Msa0300180 | Msa1205960 | 0.812539 | 4.028826e-51 | -8.615850e-47 |
| Msa0337540 | Msa1205960 | 0.848355 | 6.462204e-60 | -8.615850e-47 |
| Msa0350990 | Msa1205960 | 0.800813 | 1.208654e-48 | -8.615850e-47 |
| Msa0351100 | Msa1205960 | 0.815150 | 1.071279e-51 | -8.615850e-47 |
| Msa1111490 | Msa1205960 | 0.800815 | 1.207624e-48 | -8.615850e-47 |
| Msa1147900 | Msa1205960 | 0.814266 | 1.681518e-51 | -8.615850e-47 |
| Msa1151910 | Msa1205960 | 0.805190 | 1.504880e-49 | -8.615850e-47 |
| Msa1172450 | Msa1205960 | 0.812564 | 3.978630e-51 | -8.615850e-47 |
| Msa1173100 | Msa1205960 | 0.801361 | 9.335421e-49 | -8.615850e-47 |
| Msa1179460 | Msa1205960 | 0.806449 | 8.182115e-50 | -8.615850e-47 |
| Msa1193290 | Msa1205960 | 0.800391 | 1.473519e-48 | -8.615850e-47 |
| Msa1197590 | Msa1205960 | 0.850988 | 1.188918e-60 | -8.615850e-47 |
| Msa1201890 | Msa1205960 | 0.809567 | 1.775575e-50 | -8.615850e-47 |
| Msa1203000 | Msa1205960 | 0.804017 | 2.642817e-49 | -8.615850e-47 |
| Msa1205960 | Msa1219260 | 0.823811 | 1.132811e-53 | -8.615850e-47 |
| Msa1205960 | Msa1252140 | 0.803103 | 4.089202e-49 | -8.615850e-47 |
| Msa1205960 | Msa1254700 | 0.825318 | 5.002920e-54 | -8.615850e-47 |
| Msa1205960 | Msa1266960 | 0.817811 | 2.717468e-52 | -8.615850e-47 |
| Msa1205960 | Msa1291870 | 0.814840 | 1.255441e-51 | -8.615850e-47 |
| Msa1205960 | Msa1297180 | 0.821305 | 4.333859e-53 | -8.615850e-47 |
| Msa1205960 | Msa1303250 | 0.801615 | 8.282222e-49 | -8.615850e-47 |
| Msa1205960 | Msa1340710 | 0.801322 | 9.510934e-49 | -8.615850e-47 |
| Msa1205960 | Msa1346370 | 0.817978 | 2.491078e-52 | -8.615850e-47 |
| Msa1205960 | Msa1353470 | 0.801372 | 9.290463e-49 | -8.615850e-47 |
| Msa1205960 | Msa1397250 | 0.809100 | 2.235640e-50 | -8.615850e-47 |
| Msa1205960 | Msa1399560 | 0.809012 | 2.334600e-50 | -8.615850e-47 |
| Msa1205960 | Msa1401150 | 0.816157 | 6.389933e-52 | -8.615850e-47 |
| Msa1205960 | Msa1411020 | 0.801908 | 7.210370e-49 | -8.615850e-47 |
| Msa1205960 | Msa1443840 | 0.801031 | 1.090615e-48 | -8.615850e-47 |
| Msa1205960 | Msa1460390 | 0.824923 | 6.204420e-54 | -8.615850e-47 |
| Msa0484780 | Msa1205960 | 0.802368 | 5.799662e-49 | -8.615850e-47 |
| Msa0531430 | Msa1205960 | 0.821875 | 3.200279e-53 | -8.615850e-47 |
| Msa0534590 | Msa1205960 | 0.820601 | 6.291656e-53 | -8.615850e-47 |
| Msa0548620 | Msa1205960 | 0.810701 | 1.011518e-50 | -8.615850e-47 |
| Msa0590800 | Msa1205960 | 0.808455 | 3.072065e-50 | -8.615850e-47 |
| Msa0601750 | Msa1205960 | 0.802762 | 4.809101e-49 | -8.615850e-47 |
| Msa0871270 | Msa1205960 | 0.802663 | 5.040882e-49 | -8.615850e-47 |
| Msa0880140 | Msa1205960 | 0.834196 | 3.446203e-56 | -8.615850e-47 |
| Msa0902760 | Msa1205960 | 0.810967 | 8.856403e-51 | -8.615850e-47 |
| Msa0934890 | Msa1205960 | 0.803016 | 4.263180e-49 | -8.615850e-47 |
| Msa0938220 | Msa1205960 | 0.818442 | 1.956526e-52 | -8.615850e-47 |
| Msa0968590 | Msa1205960 | 0.800146 | 1.652949e-48 | -8.615850e-47 |
| Msa0975340 | Msa1205960 | 0.805432 | 1.338647e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1205960 | MtrunA17_Chr4g0054221 | 92.000 | 150 | 11 | 1 | 1 | 149 | 1 | 150 | 1.13e-97 | 277 |
| Msa1205960 | MtrunA17_Chr4g0054211 | 54.918 | 122 | 41 | 6 | 4 | 121 | 5 | 116 | 1.03e-32 | 112 |
| Msa1205960 | MtrunA17_Chr3g0134971 | 32.278 | 158 | 81 | 6 | 6 | 146 | 7 | 155 | 2.11e-14 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1205960 | AT5G65925.1 | 32.374 | 139 | 75 | 7 | 1 | 125 | 1 | 134 | 3.80e-11 | 58.2 |
Find 34 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTTTACCAGGAACCTTAA+TGG | 0.240275 | 8_1:-62651933 | None:intergenic |
| GGTACATGAAGGCACCATTA+AGG | 0.380437 | 8_1:+62651919 | Msa1205960:CDS |
| CGGAGTCGCAGTGTTGGAAT+TGG | 0.390004 | 8_1:+62652188 | Msa1205960:CDS |
| GATGAAAATCGTGTTGAGTT+AGG | 0.392028 | 8_1:+62652143 | Msa1205960:CDS |
| GTAGGACATCCCATGGTTGC+AGG | 0.417097 | 8_1:-62652014 | None:intergenic |
| CACAAGTGATGATGATTTCA+AGG | 0.436445 | 8_1:+62652088 | Msa1205960:CDS |
| GAAGGCACCATTAAGGTTCC+TGG | 0.439699 | 8_1:+62651926 | Msa1205960:CDS |
| CGCAACTTTACTCCTTTCCT+AGG | 0.451673 | 8_1:+62652036 | Msa1205960:CDS |
| ACCATTAAGGTTCCTGGTAA+AGG | 0.453532 | 8_1:+62651932 | Msa1205960:CDS |
| TTCTGCTTCTAGAATAAAGA+AGG | 0.463761 | 8_1:-62652271 | None:intergenic |
| CTATGTAGATCCTGCAACCA+TGG | 0.486263 | 8_1:+62652004 | Msa1205960:CDS |
| GCAAAGGAAGCAAGCTCAGT+AGG | 0.492598 | 8_1:+62651898 | Msa1205960:CDS |
| AAGGTTCCTGGTAAAGGTAA+AGG | 0.501075 | 8_1:+62651938 | Msa1205960:CDS |
| GAGTTAACACTGAAGCTCCT+AGG | 0.519744 | 8_1:-62652053 | None:intergenic |
| TCGTAAGGGCTGCGTCACTT+AGG | 0.537263 | 8_1:+62652114 | Msa1205960:CDS |
| GTGCCTCGGAGTCGCAGTGT+TGG | 0.539129 | 8_1:+62652182 | Msa1205960:CDS |
| GTAAAGGACATGTACGTCAA+TGG | 0.539882 | 8_1:+62651954 | Msa1205960:CDS |
| ACATGTCCTTTACCTTTACC+AGG | 0.540520 | 8_1:-62651944 | None:intergenic |
| ATGATTTCAAGGAACTCGTA+AGG | 0.547916 | 8_1:+62652099 | Msa1205960:CDS |
| TGAAGATGATGACAATATCA+AGG | 0.548852 | 8_1:+62652241 | Msa1205960:CDS |
| TCTGCTTCTAGAATAAAGAA+GGG | 0.562308 | 8_1:-62652270 | None:intergenic |
| CATGGTTGCAGGATCTACAT+AGG | 0.567986 | 8_1:-62652003 | None:intergenic |
| CATATTTGCTCTACTGCGAA+TGG | 0.570269 | 8_1:-62652300 | None:intergenic |
| TATGTAGATCCTGCAACCAT+GGG | 0.570418 | 8_1:+62652005 | Msa1205960:CDS |
| CTTGAAATCATCATCACTTG+TGG | 0.583473 | 8_1:-62652087 | None:intergenic |
| GTCGCAGTGTTGGAATTGGA+AGG | 0.585409 | 8_1:+62652192 | Msa1205960:CDS |
| AACACTGAAGCTCCTAGGAA+AGG | 0.606093 | 8_1:-62652048 | None:intergenic |
| GGAAAGGAGTAAAGTTGCGT+AGG | 0.616682 | 8_1:-62652032 | None:intergenic |
| CGAAAGCGATGAAAGTGCCT+CGG | 0.617025 | 8_1:+62652168 | Msa1205960:CDS |
| CAAGCTCAGTAGGTACATGA+AGG | 0.617983 | 8_1:+62651908 | Msa1205960:CDS |
| TGATTTCAAGGAACTCGTAA+GGG | 0.632665 | 8_1:+62652100 | Msa1205960:CDS |
| CTGCTTCTAGAATAAAGAAG+GGG | 0.634070 | 8_1:-62652269 | None:intergenic |
| AAGTTGCGTAGGACATCCCA+TGG | 0.638342 | 8_1:-62652021 | None:intergenic |
| ATTCCAACACTGCGACTCCG+AGG | 0.689214 | 8_1:-62652185 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAAAAAAGATAAGAGCAA+AGG | + | chr8_1:62651882-62651901 | Msa1205960:CDS | 25.0% |
| TGAAGATGATGACAATATCA+AGG | + | chr8_1:62652241-62652260 | Msa1205960:CDS | 30.0% | |
| !! | TCTGCTTCTAGAATAAAGAA+GGG | - | chr8_1:62652273-62652292 | None:intergenic | 30.0% |
| !! | TTCTGCTTCTAGAATAAAGA+AGG | - | chr8_1:62652274-62652293 | None:intergenic | 30.0% |
| AGGAAAAATCACGAGAACAT+TGG | - | chr8_1:62651986-62652005 | None:intergenic | 35.0% | |
| CTTGAAATCATCATCACTTG+TGG | - | chr8_1:62652090-62652109 | None:intergenic | 35.0% | |
| ! | CACAAGTGATGATGATTTCA+AGG | + | chr8_1:62652088-62652107 | Msa1205960:CDS | 35.0% |
| ATGATTTCAAGGAACTCGTA+AGG | + | chr8_1:62652099-62652118 | Msa1205960:CDS | 35.0% | |
| TGATTTCAAGGAACTCGTAA+GGG | + | chr8_1:62652100-62652119 | Msa1205960:CDS | 35.0% | |
| GATGAAAATCGTGTTGAGTT+AGG | + | chr8_1:62652143-62652162 | Msa1205960:CDS | 35.0% | |
| !! | CTGCTTCTAGAATAAAGAAG+GGG | - | chr8_1:62652272-62652291 | None:intergenic | 35.0% |
| ACCATTAAGGTTCCTGGTAA+AGG | + | chr8_1:62651932-62651951 | Msa1205960:CDS | 40.0% | |
| ACCTTTACCAGGAACCTTAA+TGG | - | chr8_1:62651936-62651955 | None:intergenic | 40.0% | |
| AAGGTTCCTGGTAAAGGTAA+AGG | + | chr8_1:62651938-62651957 | Msa1205960:CDS | 40.0% | |
| ACATGTCCTTTACCTTTACC+AGG | - | chr8_1:62651947-62651966 | None:intergenic | 40.0% | |
| GTAAAGGACATGTACGTCAA+TGG | + | chr8_1:62651954-62651973 | Msa1205960:CDS | 40.0% | |
| TATGTAGATCCTGCAACCAT+GGG | + | chr8_1:62652005-62652024 | Msa1205960:CDS | 40.0% | |
| CATATTTGCTCTACTGCGAA+TGG | - | chr8_1:62652303-62652322 | None:intergenic | 40.0% | |
| CAAGCTCAGTAGGTACATGA+AGG | + | chr8_1:62651908-62651927 | Msa1205960:CDS | 45.0% | |
| !! | GGTACATGAAGGCACCATTA+AGG | + | chr8_1:62651919-62651938 | Msa1205960:CDS | 45.0% |
| CATGGTTGCAGGATCTACAT+AGG | - | chr8_1:62652006-62652025 | None:intergenic | 45.0% | |
| CTATGTAGATCCTGCAACCA+TGG | + | chr8_1:62652004-62652023 | Msa1205960:CDS | 45.0% | |
| GGAAAGGAGTAAAGTTGCGT+AGG | - | chr8_1:62652035-62652054 | None:intergenic | 45.0% | |
| CGCAACTTTACTCCTTTCCT+AGG | + | chr8_1:62652036-62652055 | Msa1205960:CDS | 45.0% | |
| AACACTGAAGCTCCTAGGAA+AGG | - | chr8_1:62652051-62652070 | None:intergenic | 45.0% | |
| GAGTTAACACTGAAGCTCCT+AGG | - | chr8_1:62652056-62652075 | None:intergenic | 45.0% | |
| GCAAAGGAAGCAAGCTCAGT+AGG | + | chr8_1:62651898-62651917 | Msa1205960:CDS | 50.0% | |
| !! | GAAGGCACCATTAAGGTTCC+TGG | + | chr8_1:62651926-62651945 | Msa1205960:CDS | 50.0% |
| AAGTTGCGTAGGACATCCCA+TGG | - | chr8_1:62652024-62652043 | None:intergenic | 50.0% | |
| !! | CGAAAGCGATGAAAGTGCCT+CGG | + | chr8_1:62652168-62652187 | Msa1205960:CDS | 50.0% |
| ! | GTCGCAGTGTTGGAATTGGA+AGG | + | chr8_1:62652192-62652211 | Msa1205960:CDS | 50.0% |
| GTAGGACATCCCATGGTTGC+AGG | - | chr8_1:62652017-62652036 | None:intergenic | 55.0% | |
| TCGTAAGGGCTGCGTCACTT+AGG | + | chr8_1:62652114-62652133 | Msa1205960:CDS | 55.0% | |
| ATTCCAACACTGCGACTCCG+AGG | - | chr8_1:62652188-62652207 | None:intergenic | 55.0% | |
| ! | CGGAGTCGCAGTGTTGGAAT+TGG | + | chr8_1:62652188-62652207 | Msa1205960:CDS | 55.0% |
| GTGCCTCGGAGTCGCAGTGT+TGG | + | chr8_1:62652182-62652201 | Msa1205960:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 62651879 | 62652328 | 62651879 | ID=Msa1205960;Name=Msa1205960 |
| chr8_1 | mRNA | 62651879 | 62652328 | 62651879 | ID=Msa1205960-mRNA-1;Parent=Msa1205960;Name=Msa1205960-mRNA-1;_AED=0.29;_eAED=0.30;_QI=0|-1|0|1|-1|1|1|0|149 |
| chr8_1 | exon | 62651879 | 62652328 | 62651879 | ID=Msa1205960-mRNA-1:exon:20310;Parent=Msa1205960-mRNA-1 |
| chr8_1 | CDS | 62651879 | 62652328 | 62651879 | ID=Msa1205960-mRNA-1:cds;Parent=Msa1205960-mRNA-1 |
| Gene Sequence |
| Protein sequence |
