AlfalfaGEDB | Msa0300180

M. sativa cultivar zhongmu-4 / Msa0300180

KEGG: -
GO: GO:0000075 GO:0000077 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0006974 GO:0008150 GO:0009314 GO:0009628 GO:0009889 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010564 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031570 GO:0033043 GO:0033554 GO:0040020 GO:0043565 GO:0045786 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051445 GO:0051716 GO:0051726 GO:0051783 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2000241 GO:2001141
PFAM: PF02365.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0300180	XP_013465160.1	98.951	286	2	1	1	286	1	285	0.0	591

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0300180	sp\|O49459\|NAC73_ARATH	78.607	201	41	2	25	224	36	235	1.22e-115	337

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0300180	A0A072VM12	98.951	286	2	1	1	286	1	285	0.0	591

TFs/TRs:

Gene ID	Type	Classification
Msa0300180	TF	NAC

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0016670	Msa0300180	0.803340	3.653099e-49	-8.615850e-47
Msa0017510	Msa0300180	0.824253	8.920801e-54	-8.615850e-47
Msa0025330	Msa0300180	0.812877	3.399126e-51	-8.615850e-47
Msa0072900	Msa0300180	0.823588	1.277295e-53	-8.615850e-47
Msa0081520	Msa0300180	0.807329	5.331814e-50	-8.615850e-47
Msa0118570	Msa0300180	0.816986	4.166307e-52	-8.615850e-47
Msa0121090	Msa0300180	0.801765	7.716801e-49	-8.615850e-47
Msa0126370	Msa0300180	0.805884	1.075815e-49	-8.615850e-47
Msa0157290	Msa0300180	0.824452	8.011794e-54	-8.615850e-47
Msa0160130	Msa0300180	0.811628	6.365315e-51	-8.615850e-47
Msa0173890	Msa0300180	0.811608	6.428977e-51	-8.615850e-47
Msa0223490	Msa0300180	0.800172	1.632344e-48	-8.615850e-47
Msa0225600	Msa0300180	0.817074	3.981023e-52	-8.615850e-47
Msa0243600	Msa0300180	0.818063	2.382819e-52	-8.615850e-47
Msa0244860	Msa0300180	0.814568	1.441878e-51	-8.615850e-47
Msa0248970	Msa0300180	0.817640	2.969796e-52	-8.615850e-47
Msa0261340	Msa0300180	0.800752	1.243621e-48	-8.615850e-47
Msa0264590	Msa0300180	0.817385	3.389179e-52	-8.615850e-47
Msa0266450	Msa0300180	0.802631	5.119965e-49	-8.615850e-47
Msa0283450	Msa0300180	0.804946	1.692534e-49	-8.615850e-47
Msa0300180	Msa0300210	0.860377	2.154510e-63	-8.615850e-47
Msa0300180	Msa0319940	0.850091	2.123792e-60	-8.615850e-47
Msa0300180	Msa0332000	0.805646	1.207322e-49	-8.615850e-47
Msa0300180	Msa0337540	0.849223	3.711086e-60	-8.615850e-47
Msa0300180	Msa0339980	0.821260	4.438090e-53	-8.615850e-47
Msa0300180	Msa0388770	0.810555	1.087621e-50	-8.615850e-47
Msa0300180	Msa0410650	0.821748	3.423538e-53	-8.615850e-47
Msa0300180	Msa0417880	0.802572	5.264090e-49	-8.615850e-47
Msa0300180	Msa0507680	0.815355	9.647015e-52	-8.615850e-47
Msa0300180	Msa0532070	0.803376	3.591310e-49	-8.615850e-47
Msa0300180	Msa0546680	0.800864	1.179912e-48	-8.615850e-47
Msa0300180	Msa0548620	0.838009	3.708465e-57	-8.615850e-47
Msa0300180	Msa0548660	0.818299	2.107252e-52	-8.615850e-47
Msa0300180	Msa0574890	0.810644	1.040336e-50	-8.615850e-47
Msa0300180	Msa0609430	0.820095	8.222109e-53	-8.615850e-47
Msa0300180	Msa0626450	0.800508	1.394883e-48	-8.615850e-47
Msa0300180	Msa0626490	0.803213	3.880786e-49	-8.615850e-47
Msa0300180	Msa0639430	0.802510	5.422889e-49	-8.615850e-47
Msa0300180	Msa0650070	0.807091	5.985901e-50	-8.615850e-47
Msa0300180	Msa0683570	0.801101	1.055220e-48	-8.615850e-47
Msa0300180	Msa0684500	0.822569	2.208092e-53	-8.615850e-47
Msa0300180	Msa0692060	0.806355	8.562234e-50	-8.615850e-47
Msa0300180	Msa0698770	0.828124	1.070121e-54	-8.615850e-47
Msa0300180	Msa0880140	0.824125	9.563281e-54	-8.615850e-47
Msa0300180	Msa0902760	0.803180	3.942439e-49	-8.615850e-47
Msa0300180	Msa0974060	0.824816	6.573999e-54	-8.615850e-47
Msa0300180	Msa1013400	0.810205	1.293929e-50	-8.615850e-47
Msa0300180	Msa1020630	0.812486	4.138588e-51	-8.615850e-47
Msa0300180	Msa1024740	0.818785	1.635047e-52	-8.615850e-47
Msa0300180	Msa1063500	0.830434	2.943900e-55	-8.615850e-47
Msa0300180	Msa1082500	0.805711	1.169799e-49	-8.615850e-47
Msa0300180	Msa1085200	0.823257	1.527021e-53	-8.615850e-47
Msa0300180	Msa1093530	0.802683	4.994687e-49	-8.615850e-47
Msa0300180	Msa1108790	0.800084	1.700934e-48	-8.615850e-47
Msa0300180	Msa1113800	0.827592	1.437156e-54	-8.615850e-47
Msa0300180	Msa1147900	0.805522	1.281575e-49	-8.615850e-47
Msa0300180	Msa1151910	0.808367	3.206506e-50	-8.615850e-47
Msa0300180	Msa1160500	0.820274	7.478758e-53	-8.615850e-47
Msa0300180	Msa1182860	0.815616	8.436423e-52	-8.615850e-47
Msa0300180	Msa1197590	0.814349	1.611672e-51	-8.615850e-47
Msa0300180	Msa1201890	0.811631	6.356282e-51	-8.615850e-47
Msa0300180	Msa1205960	0.812539	4.028826e-51	-8.615850e-47
Msa0300180	Msa1209460	0.801917	7.179751e-49	-8.615850e-47
Msa0300180	Msa1254700	0.805791	1.125401e-49	-8.615850e-47
Msa0300180	Msa1292830	0.815908	7.265518e-52	-8.615850e-47
Msa0300180	Msa1340710	0.802318	5.939423e-49	-8.615850e-47
Msa0300180	Msa1409870	0.805210	1.490000e-49	-8.615850e-47
Msa0300180	Msa1442820	0.807709	4.426792e-50	-8.615850e-47
Msa0300180	Msa1460390	0.804843	1.777733e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0300180	MtrunA17_Chr2g0324961	98.951	286	2	1	1	286	1	285	0.0	591
Msa0300180	MtrunA17_Chr4g0040541	76.552	290	58	5	1	286	1	284	8.84e-157	438
Msa0300180	MtrunA17_Chr4g0046431	64.561	285	87	7	9	285	26	304	1.82e-124	357
Msa0300180	MtrunA17_Chr2g0282871	57.770	296	112	5	1	286	1	293	3.08e-115	333
Msa0300180	MtrunA17_Chr8g0367761	82.682	179	30	1	26	204	46	223	1.75e-108	315
Msa0300180	MtrunA17_Chr8g0389661	73.232	198	41	4	27	217	30	222	1.63e-95	290
Msa0300180	MtrunA17_Chr1g0198751	57.592	191	77	2	25	215	37	223	1.82e-70	225
Msa0300180	MtrunA17_Chr5g0422431	54.950	202	87	2	20	221	32	229	4.19e-70	223
Msa0300180	MtrunA17_Chr7g0227071	55.135	185	79	2	25	208	34	215	1.29e-63	207
Msa0300180	MtrunA17_Chr1g0198741	56.250	112	49	0	104	215	7	118	2.51e-35	128
Msa0300180	MtrunA17_Chr1g0147421	32.500	200	105	9	49	240	27	204	1.97e-15	75.5
Msa0300180	MtrunA17_Chr6g0484441	32.961	179	93	7	48	217	4	164	1.68e-14	72.4
Msa0300180	MtrunA17_Chr7g0253011	33.503	197	94	9	49	227	17	194	1.79e-14	72.8
Msa0300180	MtrunA17_Chr3g0091921	32.240	183	95	7	49	220	16	180	1.43e-13	69.7
Msa0300180	MtrunA17_Chr4g0060931	32.164	171	87	7	49	209	17	168	4.34e-13	68.6
Msa0300180	MtrunA17_Chr7g0217711	32.857	140	77	4	51	189	21	144	9.68e-13	65.5
Msa0300180	MtrunA17_Chr2g0318101	28.834	163	96	6	34	193	1	146	5.08e-12	65.5
Msa0300180	MtrunA17_Chr3g0130731	34.247	146	74	7	49	191	7	133	2.39e-11	63.2
Msa0300180	MtrunA17_Chr8g0384721	32.877	146	76	6	49	191	7	133	3.19e-11	62.8
Msa0300180	MtrunA17_Chr2g0316971	31.548	168	88	7	49	206	23	173	3.34e-11	63.2
Msa0300180	MtrunA17_Chr1g0183741	27.711	166	97	6	48	208	9	156	3.47e-11	62.4
Msa0300180	MtrunA17_Chr3g0124761	33.333	144	78	6	49	191	7	133	3.98e-11	62.4
Msa0300180	MtrunA17_Chr8g0385991	32.432	148	79	7	49	193	14	143	6.33e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0300180	AT4G28500.1	78.607	201	41	2	25	224	36	235	1.24e-116	337
Msa0300180	AT1G28470.1	75.773	194	45	1	24	215	50	243	4.39e-105	308
Msa0300180	AT4G29230.3	70.647	201	46	2	25	217	29	224	5.28e-97	292
Msa0300180	AT4G29230.1	70.647	201	46	2	25	217	29	224	1.66e-96	293
Msa0300180	AT4G29230.2	70.647	201	45	2	25	217	29	223	4.09e-96	292
Msa0300180	AT5G56620.3	67.839	199	49	4	27	214	25	219	3.75e-90	274
Msa0300180	AT5G56620.2	67.839	199	49	4	27	214	25	219	4.68e-90	273
Msa0300180	AT5G56620.1	67.839	199	49	4	27	214	25	219	7.61e-90	272
Msa0300180	AT1G25580.1	56.614	189	72	4	25	213	37	215	2.29e-65	211
Msa0300180	AT5G14490.2	50.262	191	86	4	30	213	30	218	1.82e-54	178
Msa0300180	AT5G14490.4	50.262	191	86	4	30	213	30	218	2.99e-54	179
Msa0300180	AT5G14490.1	50.262	191	86	4	30	213	33	221	3.44e-54	179
Msa0300180	AT5G14490.3	50.262	191	86	4	30	213	33	221	3.96e-54	180
Msa0300180	AT3G01600.1	50.532	188	85	3	30	210	30	216	2.02e-53	178
Msa0300180	AT3G04060.1	33.520	179	95	6	41	211	12	174	8.35e-16	77.0
Msa0300180	AT5G18270.2	35.196	179	90	7	42	211	14	175	1.05e-15	76.6
Msa0300180	AT5G18270.1	35.196	179	90	7	42	211	14	175	1.60e-15	75.9
Msa0300180	AT3G18400.1	34.940	166	82	8	48	206	4	150	1.37e-14	73.2
Msa0300180	AT5G07680.2	32.738	168	90	5	49	209	3	154	1.56e-13	70.1
Msa0300180	AT3G29035.1	33.529	170	82	8	49	207	24	173	1.61e-13	70.1
Msa0300180	AT5G07680.1	32.738	168	90	5	49	209	17	168	1.62e-13	70.1
Msa0300180	AT5G39610.1	34.524	168	81	7	49	206	20	168	2.91e-13	68.9
Msa0300180	AT5G61430.1	32.544	169	89	6	49	209	16	167	4.33e-13	68.9
Msa0300180	AT1G01720.1	32.000	150	84	5	43	191	1	133	8.67e-13	67.8
Msa0300180	AT5G08790.1	33.562	146	75	6	49	191	7	133	7.35e-12	64.7
Msa0300180	AT1G02230.1	32.192	146	74	7	50	188	4	131	1.49e-11	64.7
Msa0300180	AT4G35580.3	29.592	196	105	10	42	226	2	175	2.32e-11	64.3
Msa0300180	AT4G35580.2	29.592	196	105	10	42	226	2	175	2.37e-11	64.3
Msa0300180	AT4G35580.1	29.592	196	105	10	42	226	2	175	2.59e-11	64.3
Msa0300180	AT1G76420.1	31.285	179	93	9	48	216	21	179	2.61e-11	63.5
Msa0300180	AT1G33060.2	29.878	164	88	8	42	198	17	160	3.32e-11	63.9
Msa0300180	AT1G33060.1	29.878	164	88	8	42	198	17	160	3.43e-11	63.9
Msa0300180	AT1G02220.1	32.394	142	76	6	50	188	4	128	8.60e-11	62.4

Find 87 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTTCTTCAAATTGTAATTA+AGG	0.185444	2_3:+55573472	None:intergenic
AACTTGCCACTCACGAAAAT+TGG	0.190888	2_3:+55573918	None:intergenic
TTCTTAATTCCTCTTACTAA+TGG	0.263928	2_3:+55575068	None:intergenic
TCTTAATTCCTCTTACTAAT+GGG	0.282660	2_3:+55575069	None:intergenic
TTATACATAGAAATGGAATA+AGG	0.294893	2_3:-55574780	Msa0300180:five_prime_UTR
TCAAGAGATTCTTGAACATT+TGG	0.312906	2_3:-55574346	Msa0300180:CDS
GAAAGCCAGAGAAAACAAAT+TGG	0.314159	2_3:-55573845	Msa0300180:CDS
AGCCTTTGTTATGCATGTTT+TGG	0.339605	2_3:+55573261	None:intergenic
CACATTGTCTAGGCTGTGTT+TGG	0.340883	2_3:+55573748	None:intergenic
AGGCTTGATGCCTTTATACT+TGG	0.353127	2_3:-55573243	Msa0300180:three_prime_UTR
TTCGTGAGTGGCAAGTTAAA+AGG	0.353219	2_3:-55573912	Msa0300180:CDS
TTGTAGTTCAAGACTGCTTA+AGG	0.353973	2_3:+55573179	None:intergenic
AGAGGAGAAAGAAGGAGAAT+TGG	0.354646	2_3:-55573790	Msa0300180:CDS
TAATCATAGTACTATCCTTA+TGG	0.361262	2_3:-55574716	Msa0300180:CDS
TAATATTTACCCAAGTATAA+AGG	0.364369	2_3:+55573233	None:intergenic
AATTGACCTGGTAACTTCTC+TGG	0.367159	2_3:+55574228	None:intergenic
CATTATTTCCAAGATGGTAT+TGG	0.373788	2_3:+55573814	None:intergenic
GAAGAACTTGTCTCTTCTAA+AGG	0.383819	2_3:+55574925	None:intergenic
CTCTCTCTCTGTCTCCCACT+TGG	0.387584	2_3:-55574837	Msa0300180:five_prime_UTR
GAGTTACCTGGATTACCTGC+TGG	0.403541	2_3:-55574390	Msa0300180:CDS
AAGAACTTGTCTCTTCTAAA+GGG	0.412652	2_3:+55574926	None:intergenic
CTGCTTCATCTGAAAACTAA+TGG	0.421652	2_3:-55573410	Msa0300180:three_prime_UTR
GGGATGAACTCATCAATTAA+AGG	0.427373	2_3:+55574288	None:intergenic
TATGCAGGTGTCGGCAAAGA+TGG	0.429616	2_3:-55574065	Msa0300180:intron
CCTCTTCATTATTTCCAAGA+TGG	0.434430	2_3:+55573808	None:intergenic
GAGTTCATCCCTACTCTTGA+AGG	0.437508	2_3:-55574276	Msa0300180:CDS
GAGACAAGATGGCATAAAAC+AGG	0.437609	2_3:-55573951	Msa0300180:CDS
TATACATAGAAATGGAATAA+GGG	0.441055	2_3:-55574779	Msa0300180:five_prime_UTR
CTGTGTTTGGTAGAAAACTT+TGG	0.443831	2_3:+55573761	None:intergenic
CCTACTCTTGAAGGAGAGAA+TGG	0.452976	2_3:-55574267	Msa0300180:CDS
ATAACAAACAGTAGTTTAAG+AGG	0.466751	2_3:-55573443	Msa0300180:three_prime_UTR
AGAGATTCTTGAACATTTGG+AGG	0.472757	2_3:-55574343	Msa0300180:CDS
AGATAGTTTGAGTTAAGAGA+TGG	0.473289	2_3:+55574862	None:intergenic
TCATGCCACTTCTTGTTGAA+AGG	0.473832	2_3:+55574958	None:intergenic
TCATTCACCCCATTAGTAAG+AGG	0.474578	2_3:-55575077	None:intergenic
CCATCGAAAGCATACACAAC+AGG	0.476509	2_3:-55574017	Msa0300180:CDS
TTCACCCCAGCAGGTAATCC+AGG	0.479471	2_3:+55574384	None:intergenic
CCATTCTCTCCTTCAAGAGT+AGG	0.481831	2_3:+55574267	None:intergenic
GGATCAAACTTCACCCCAGC+AGG	0.493343	2_3:+55574375	None:intergenic
GTTATGCACCAATACCATCT+TGG	0.493624	2_3:-55573822	Msa0300180:CDS
CATTCTCTCCTTCAAGAGTA+GGG	0.494352	2_3:+55574268	None:intergenic
GGCTTGATGCCTTTATACTT+GGG	0.495299	2_3:-55573242	Msa0300180:three_prime_UTR
TATCTTTGCGTCTCTCCATA+AGG	0.495599	2_3:+55574701	None:intergenic
TGATCCGACATTTCTTTCAC+AGG	0.495756	2_3:-55574040	Msa0300180:CDS
AGTTACCTGGATTACCTGCT+GGG	0.500946	2_3:-55574389	Msa0300180:CDS
TCATATCAAATGCCAAGATC+AGG	0.502996	2_3:-55574647	Msa0300180:intron
AAAGCCAGAGAAAACAAATT+GGG	0.504516	2_3:-55573844	Msa0300180:CDS
GAATGTGAAGAACCTGATCT+TGG	0.505797	2_3:+55574635	None:intergenic
GTTAATGAATTATAGGGTGC+TGG	0.506242	2_3:-55574420	Msa0300180:intron
CCTGTTGTGTATGCTTTCGA+TGG	0.507588	2_3:+55574017	None:intergenic
TTCCACCTTTCAACAAGAAG+TGG	0.515646	2_3:-55574963	Msa0300180:five_prime_UTR
CTTAATTCCTCTTACTAATG+GGG	0.519058	2_3:+55575070	None:intergenic
AATAATGAAGAGGAGAAAGA+AGG	0.526318	2_3:-55573798	Msa0300180:CDS
TAACAAACAGTAGTTTAAGA+GGG	0.529081	2_3:-55573442	Msa0300180:three_prime_UTR
TGCCACTTCTTGTTGAAAGG+TGG	0.541565	2_3:+55574961	None:intergenic
TCAAGAATCTCTTGATCAGT+AGG	0.542994	2_3:+55574354	None:intergenic
CTTCTTGTTGAAAGGTGGAA+AGG	0.546015	2_3:+55574966	None:intergenic
GCTGGAATTCATGAGTTACC+TGG	0.547070	2_3:-55574402	Msa0300180:CDS
CAGATGGAAGTGAGACAAGA+TGG	0.555906	2_3:-55573962	Msa0300180:CDS
AAAGATGTACCATCATGGAC+AGG	0.570055	2_3:+55573540	None:intergenic
TTCAACAAGAAGTGGCATGA+GGG	0.571306	2_3:-55574955	Msa0300180:five_prime_UTR
ATTAGTAAGAGGAATTAAGA+AGG	0.576149	2_3:-55575066	Msa0300180:five_prime_UTR
CATCTGAAAACTAATGGTGA+GGG	0.578479	2_3:-55573404	Msa0300180:three_prime_UTR
GAATGAAAGATGTACCATCA+TGG	0.578897	2_3:+55573535	None:intergenic
TAACATGAAATCGTGATGCA+TGG	0.580045	2_3:-55573497	Msa0300180:three_prime_UTR
ACTCATCCAGAGAAGTTACC+AGG	0.581252	2_3:-55574234	Msa0300180:intron
TCATCTGAAAACTAATGGTG+AGG	0.589188	2_3:-55573405	Msa0300180:three_prime_UTR
TTGATATGATGACCACATGT+AGG	0.596208	2_3:+55574661	None:intergenic
AATGAACCACCACATTGTCT+AGG	0.596526	2_3:+55573738	None:intergenic
TGTAGTTAGTGTAAAGCACA+AGG	0.598633	2_3:+55573877	None:intergenic
ATGGCCTGTGAAAGAAATGT+CGG	0.608865	2_3:+55574036	None:intergenic
CTAATGGTGAGGGATAAGTA+AGG	0.613968	2_3:-55573394	Msa0300180:three_prime_UTR
TCAACAAGAAGTGGCATGAG+GGG	0.619040	2_3:-55574954	Msa0300180:five_prime_UTR
AACATTTGGAGGCAAAAGTG+AGG	0.622163	2_3:-55574332	Msa0300180:CDS
CACCAAAACATGCATAACAA+AGG	0.630371	2_3:-55573263	Msa0300180:three_prime_UTR
CCATCTTGGAAATAATGAAG+AGG	0.636615	2_3:-55573808	Msa0300180:CDS
TTTCAACAAGAAGTGGCATG+AGG	0.638930	2_3:-55574956	Msa0300180:five_prime_UTR
ATCAGAACTTGTCCTACATG+TGG	0.643015	2_3:-55574673	Msa0300180:CDS
AACAAACAGTAGTTTAAGAG+GGG	0.647446	2_3:-55573441	Msa0300180:three_prime_UTR
AAAGTGCACACAGATGCAGA+TGG	0.660294	2_3:-55573978	Msa0300180:CDS
GGCATAAAACAGGTAAAACA+AGG	0.665333	2_3:-55573941	Msa0300180:CDS
CAAACACAGCCTAGACAATG+TGG	0.666262	2_3:-55573747	Msa0300180:CDS
ACATAGAAATGGAATAAGGG+AGG	0.680191	2_3:-55574776	Msa0300180:five_prime_UTR
ACACAGCCTAGACAATGTGG+TGG	0.735435	2_3:-55573744	Msa0300180:CDS
TCAAGGTGTGAATCATGAGG+TGG	0.742861	2_3:-55573670	Msa0300180:CDS
TGATCAAGGTGTGAATCATG+AGG	0.746754	2_3:-55573673	Msa0300180:CDS
GTTACCTGGATTACCTGCTG+GGG	0.752999	2_3:-55574388	Msa0300180:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATGATATTATTATTTTTCTT+TGG	-	chr2_3:55573706-55573725	Msa0300180:CDS	10.0%
!!	TACTAATAATATAAATATTG+TGG	-	chr2_3:55573762-55573781	Msa0300180:CDS	10.0%
!!!	ATGATATTATTATTTTTCTT+TGG	-	chr2_3:55573706-55573725	Msa0300180:CDS	10.0%
!!	TACTAATAATATAAATATTG+TGG	-	chr2_3:55573762-55573781	Msa0300180:CDS	10.0%
!!!	TCTTTTATTATACATAGAAA+TGG	-	chr2_3:55573419-55573438	Msa0300180:three_prime_UTR	15.0%
!!	ATAAGGCATATATATATATT+TGG	+	chr2_3:55574011-55574030	None:intergenic	15.0%
!!!	TTTTCTGATAAAAAACTAAA+TGG	-	chr2_3:55574510-55574529	Msa0300180:intron	15.0%
!!	ATTTCTTCAAATTGTAATTA+AGG	+	chr2_3:55574737-55574756	None:intergenic	15.0%
!!!	TCTTTTATTATACATAGAAA+TGG	-	chr2_3:55573419-55573438	Msa0300180:three_prime_UTR	15.0%
!!	ATAAGGCATATATATATATT+TGG	+	chr2_3:55574011-55574030	None:intergenic	15.0%
!!!	TTTTCTGATAAAAAACTAAA+TGG	-	chr2_3:55574510-55574529	Msa0300180:intron	15.0%
!!	ATTTCTTCAAATTGTAATTA+AGG	+	chr2_3:55574737-55574756	None:intergenic	15.0%
!!!	AAATTCTACCTTACTTTTTT+TGG	+	chr2_3:55573216-55573235	None:intergenic	20.0%
!!	TTATACATAGAAATGGAATA+AGG	-	chr2_3:55573426-55573445	Msa0300180:three_prime_UTR	20.0%
!!	TATACATAGAAATGGAATAA+GGG	-	chr2_3:55573427-55573446	Msa0300180:three_prime_UTR	20.0%
!!!	TAGTACTATGATTATTTTCA+GGG	+	chr2_3:55573484-55573503	None:intergenic	20.0%
!!!	ATAGTACTATGATTATTTTC+AGG	+	chr2_3:55573485-55573504	None:intergenic	20.0%
!!!	TTAAAGCTTTAACAAAATCA+CGG	+	chr2_3:55573642-55573661	None:intergenic	20.0%
!!	GAAATGGAAAGTAAATTATA+AGG	+	chr2_3:55574028-55574047	None:intergenic	20.0%
!!	CTATGAAAGAATAAAAGAAA+TGG	+	chr2_3:55574044-55574063	None:intergenic	20.0%
!!	ATTATATAGGATATAAGATG+AGG	+	chr2_3:55574083-55574102	None:intergenic	20.0%
!!	CACAAAAAATGTCATTATAT+AGG	+	chr2_3:55574096-55574115	None:intergenic	20.0%
!!!	CATGTTAATATTTTCTTTCA+AGG	+	chr2_3:55574696-55574715	None:intergenic	20.0%
!!	TAATATTTACCCAAGTATAA+AGG	+	chr2_3:55574976-55574995	None:intergenic	20.0%
!!!	AAATTCTACCTTACTTTTTT+TGG	+	chr2_3:55573216-55573235	None:intergenic	20.0%
!!	TTATACATAGAAATGGAATA+AGG	-	chr2_3:55573426-55573445	Msa0300180:three_prime_UTR	20.0%
!!	TATACATAGAAATGGAATAA+GGG	-	chr2_3:55573427-55573446	Msa0300180:three_prime_UTR	20.0%
!!!	TAGTACTATGATTATTTTCA+GGG	+	chr2_3:55573484-55573503	None:intergenic	20.0%
!!!	ATAGTACTATGATTATTTTC+AGG	+	chr2_3:55573485-55573504	None:intergenic	20.0%
!!!	TTAAAGCTTTAACAAAATCA+CGG	+	chr2_3:55573642-55573661	None:intergenic	20.0%
!!	GAAATGGAAAGTAAATTATA+AGG	+	chr2_3:55574028-55574047	None:intergenic	20.0%
!!	CTATGAAAGAATAAAAGAAA+TGG	+	chr2_3:55574044-55574063	None:intergenic	20.0%
!!	ATTATATAGGATATAAGATG+AGG	+	chr2_3:55574083-55574102	None:intergenic	20.0%
!!	CACAAAAAATGTCATTATAT+AGG	+	chr2_3:55574096-55574115	None:intergenic	20.0%
!!!	CATGTTAATATTTTCTTTCA+AGG	+	chr2_3:55574696-55574715	None:intergenic	20.0%
!!	TAATATTTACCCAAGTATAA+AGG	+	chr2_3:55574976-55574995	None:intergenic	20.0%
!	TCTTAATTCCTCTTACTAAT+GGG	+	chr2_3:55573140-55573159	None:intergenic	25.0%
!	TTCTTAATTCCTCTTACTAA+TGG	+	chr2_3:55573141-55573160	None:intergenic	25.0%
!	ATTAGTAAGAGGAATTAAGA+AGG	-	chr2_3:55573140-55573159	Msa0300180:three_prime_UTR	25.0%
!	ATCAAGTACCAAAAAAAGTA+AGG	-	chr2_3:55573205-55573224	Msa0300180:three_prime_UTR	25.0%
!	TAATCATAGTACTATCCTTA+TGG	-	chr2_3:55573490-55573509	Msa0300180:three_prime_UTR	25.0%
!!!	TGGTGATGTTAATGATTTTA+TGG	-	chr2_3:55573726-55573745	Msa0300180:CDS	25.0%
!!	TTGTGGTGTTAATGAATTAT+AGG	-	chr2_3:55573779-55573798	Msa0300180:CDS	25.0%
!!	TGTGGTGTTAATGAATTATA+GGG	-	chr2_3:55573780-55573799	Msa0300180:CDS	25.0%
!	AGGCATATATATATATTTGG+TGG	+	chr2_3:55574008-55574027	None:intergenic	25.0%
!!	ATATTTTACGTCTAATATGC+AGG	-	chr2_3:55574126-55574145	Msa0300180:intron	25.0%
!	AAAAAACTAAATGGTGATCA+AGG	-	chr2_3:55574519-55574538	Msa0300180:intron	25.0%
!!	TCTTTCATATCTTTTGATCA+AGG	-	chr2_3:55574600-55574619	Msa0300180:intron	25.0%
!	ATAACAAACAGTAGTTTAAG+AGG	-	chr2_3:55574763-55574782	Msa0300180:exon	25.0%
!	TAACAAACAGTAGTTTAAGA+GGG	-	chr2_3:55574764-55574783	Msa0300180:exon	25.0%
!	TCTTAATTCCTCTTACTAAT+GGG	+	chr2_3:55573140-55573159	None:intergenic	25.0%
!	TTCTTAATTCCTCTTACTAA+TGG	+	chr2_3:55573141-55573160	None:intergenic	25.0%
!	ATTAGTAAGAGGAATTAAGA+AGG	-	chr2_3:55573140-55573159	Msa0300180:three_prime_UTR	25.0%
!	ATCAAGTACCAAAAAAAGTA+AGG	-	chr2_3:55573205-55573224	Msa0300180:three_prime_UTR	25.0%
!	TAATCATAGTACTATCCTTA+TGG	-	chr2_3:55573490-55573509	Msa0300180:three_prime_UTR	25.0%
!!!	TGGTGATGTTAATGATTTTA+TGG	-	chr2_3:55573726-55573745	Msa0300180:CDS	25.0%
!!	TTGTGGTGTTAATGAATTAT+AGG	-	chr2_3:55573779-55573798	Msa0300180:CDS	25.0%
!!	TGTGGTGTTAATGAATTATA+GGG	-	chr2_3:55573780-55573799	Msa0300180:CDS	25.0%
!	AGGCATATATATATATTTGG+TGG	+	chr2_3:55574008-55574027	None:intergenic	25.0%
!!	ATATTTTACGTCTAATATGC+AGG	-	chr2_3:55574126-55574145	Msa0300180:intron	25.0%
!	AAAAAACTAAATGGTGATCA+AGG	-	chr2_3:55574519-55574538	Msa0300180:intron	25.0%
!!	TCTTTCATATCTTTTGATCA+AGG	-	chr2_3:55574600-55574619	Msa0300180:intron	25.0%
!	ATAACAAACAGTAGTTTAAG+AGG	-	chr2_3:55574763-55574782	Msa0300180:exon	25.0%
!	TAACAAACAGTAGTTTAAGA+GGG	-	chr2_3:55574764-55574783	Msa0300180:exon	25.0%
	AAGAACTTGTCTCTTCTAAA+GGG	+	chr2_3:55573283-55573302	None:intergenic	30.0%
!	AGATAGTTTGAGTTAAGAGA+TGG	+	chr2_3:55573347-55573366	None:intergenic	30.0%
	TCAAGAGATTCTTGAACATT+TGG	-	chr2_3:55573860-55573879	Msa0300180:CDS	30.0%
	AAAGCCAGAGAAAACAAATT+GGG	-	chr2_3:55574362-55574381	Msa0300180:CDS	30.0%
!!	ATAACCCAATTTGTTTTCTC+TGG	+	chr2_3:55574369-55574388	None:intergenic	30.0%
	CATTATTTCCAAGATGGTAT+TGG	+	chr2_3:55574395-55574414	None:intergenic	30.0%
	AATAATGAAGAGGAGAAAGA+AGG	-	chr2_3:55574408-55574427	Msa0300180:CDS	30.0%
	GTGGTTAATCATAAAAACAG+TGG	-	chr2_3:55574555-55574574	Msa0300180:intron	30.0%
	AACAAACAGTAGTTTAAGAG+GGG	-	chr2_3:55574765-55574784	Msa0300180:five_prime_UTR	30.0%
!!!	TACAATTTTTGAAGTGTAGC+TGG	-	chr2_3:55574900-55574919	Msa0300180:five_prime_UTR	30.0%
	AAGAACTTGTCTCTTCTAAA+GGG	+	chr2_3:55573283-55573302	None:intergenic	30.0%
!	AGATAGTTTGAGTTAAGAGA+TGG	+	chr2_3:55573347-55573366	None:intergenic	30.0%
	TCAAGAGATTCTTGAACATT+TGG	-	chr2_3:55573860-55573879	Msa0300180:CDS	30.0%
	AAAGCCAGAGAAAACAAATT+GGG	-	chr2_3:55574362-55574381	Msa0300180:CDS	30.0%
!!	ATAACCCAATTTGTTTTCTC+TGG	+	chr2_3:55574369-55574388	None:intergenic	30.0%
	CATTATTTCCAAGATGGTAT+TGG	+	chr2_3:55574395-55574414	None:intergenic	30.0%
	AATAATGAAGAGGAGAAAGA+AGG	-	chr2_3:55574408-55574427	Msa0300180:CDS	30.0%
	GTGGTTAATCATAAAAACAG+TGG	-	chr2_3:55574555-55574574	Msa0300180:intron	30.0%
	AACAAACAGTAGTTTAAGAG+GGG	-	chr2_3:55574765-55574784	Msa0300180:five_prime_UTR	30.0%
!!!	TACAATTTTTGAAGTGTAGC+TGG	-	chr2_3:55574900-55574919	Msa0300180:five_prime_UTR	30.0%
	GAAGAACTTGTCTCTTCTAA+AGG	+	chr2_3:55573284-55573303	None:intergenic	35.0%
	ACATAGAAATGGAATAAGGG+AGG	-	chr2_3:55573430-55573449	Msa0300180:three_prime_UTR	35.0%
	TTGATATGATGACCACATGT+AGG	+	chr2_3:55573548-55573567	None:intergenic	35.0%
	TCATATCAAATGCCAAGATC+AGG	-	chr2_3:55573559-55573578	Msa0300180:CDS	35.0%
	CTTTAACAAAATCACGGTGT+CGG	+	chr2_3:55573636-55573655	None:intergenic	35.0%
	GTTAATGAATTATAGGGTGC+TGG	-	chr2_3:55573786-55573805	Msa0300180:CDS	35.0%
	TCAAGAATCTCTTGATCAGT+AGG	+	chr2_3:55573855-55573874	None:intergenic	35.0%
	AGAGATTCTTGAACATTTGG+AGG	-	chr2_3:55573863-55573882	Msa0300180:CDS	35.0%
	GGGATGAACTCATCAATTAA+AGG	+	chr2_3:55573921-55573940	None:intergenic	35.0%
	GGCATAAAACAGGTAAAACA+AGG	-	chr2_3:55574265-55574284	Msa0300180:CDS	35.0%
	TGTAGTTAGTGTAAAGCACA+AGG	+	chr2_3:55574332-55574351	None:intergenic	35.0%
	GAAAGCCAGAGAAAACAAAT+TGG	-	chr2_3:55574361-55574380	Msa0300180:CDS	35.0%
	CCTCTTCATTATTTCCAAGA+TGG	+	chr2_3:55574401-55574420	None:intergenic	35.0%
	CCATCTTGGAAATAATGAAG+AGG	-	chr2_3:55574398-55574417	Msa0300180:CDS	35.0%
	CTGTGTTTGGTAGAAAACTT+TGG	+	chr2_3:55574448-55574467	None:intergenic	35.0%
	GAATGAAAGATGTACCATCA+TGG	+	chr2_3:55574674-55574693	None:intergenic	35.0%
	TAACATGAAATCGTGATGCA+TGG	-	chr2_3:55574709-55574728	Msa0300180:CDS	35.0%
	CTGCTTCATCTGAAAACTAA+TGG	-	chr2_3:55574796-55574815	Msa0300180:five_prime_UTR	35.0%
	TCATCTGAAAACTAATGGTG+AGG	-	chr2_3:55574801-55574820	Msa0300180:five_prime_UTR	35.0%
	CATCTGAAAACTAATGGTGA+GGG	-	chr2_3:55574802-55574821	Msa0300180:five_prime_UTR	35.0%
	CACCAAAACATGCATAACAA+AGG	-	chr2_3:55574943-55574962	Msa0300180:five_prime_UTR	35.0%
!!	AGCCTTTGTTATGCATGTTT+TGG	+	chr2_3:55574948-55574967	None:intergenic	35.0%
	TTGTAGTTCAAGACTGCTTA+AGG	+	chr2_3:55575030-55575049	None:intergenic	35.0%
	GAAGAACTTGTCTCTTCTAA+AGG	+	chr2_3:55573284-55573303	None:intergenic	35.0%
	ACATAGAAATGGAATAAGGG+AGG	-	chr2_3:55573430-55573449	Msa0300180:three_prime_UTR	35.0%
	TTGATATGATGACCACATGT+AGG	+	chr2_3:55573548-55573567	None:intergenic	35.0%
	TCATATCAAATGCCAAGATC+AGG	-	chr2_3:55573559-55573578	Msa0300180:CDS	35.0%
	CTTTAACAAAATCACGGTGT+CGG	+	chr2_3:55573636-55573655	None:intergenic	35.0%
	GTTAATGAATTATAGGGTGC+TGG	-	chr2_3:55573786-55573805	Msa0300180:CDS	35.0%
	TCAAGAATCTCTTGATCAGT+AGG	+	chr2_3:55573855-55573874	None:intergenic	35.0%
	AGAGATTCTTGAACATTTGG+AGG	-	chr2_3:55573863-55573882	Msa0300180:CDS	35.0%
	GGGATGAACTCATCAATTAA+AGG	+	chr2_3:55573921-55573940	None:intergenic	35.0%
	GGCATAAAACAGGTAAAACA+AGG	-	chr2_3:55574265-55574284	Msa0300180:CDS	35.0%
	TGTAGTTAGTGTAAAGCACA+AGG	+	chr2_3:55574332-55574351	None:intergenic	35.0%
	GAAAGCCAGAGAAAACAAAT+TGG	-	chr2_3:55574361-55574380	Msa0300180:CDS	35.0%
	CCTCTTCATTATTTCCAAGA+TGG	+	chr2_3:55574401-55574420	None:intergenic	35.0%
	CCATCTTGGAAATAATGAAG+AGG	-	chr2_3:55574398-55574417	Msa0300180:CDS	35.0%
	CTGTGTTTGGTAGAAAACTT+TGG	+	chr2_3:55574448-55574467	None:intergenic	35.0%
	GAATGAAAGATGTACCATCA+TGG	+	chr2_3:55574674-55574693	None:intergenic	35.0%
	TAACATGAAATCGTGATGCA+TGG	-	chr2_3:55574709-55574728	Msa0300180:CDS	35.0%
	CTGCTTCATCTGAAAACTAA+TGG	-	chr2_3:55574796-55574815	Msa0300180:five_prime_UTR	35.0%
	TCATCTGAAAACTAATGGTG+AGG	-	chr2_3:55574801-55574820	Msa0300180:five_prime_UTR	35.0%
	CATCTGAAAACTAATGGTGA+GGG	-	chr2_3:55574802-55574821	Msa0300180:five_prime_UTR	35.0%
	CACCAAAACATGCATAACAA+AGG	-	chr2_3:55574943-55574962	Msa0300180:five_prime_UTR	35.0%
!!	AGCCTTTGTTATGCATGTTT+TGG	+	chr2_3:55574948-55574967	None:intergenic	35.0%
	TTGTAGTTCAAGACTGCTTA+AGG	+	chr2_3:55575030-55575049	None:intergenic	35.0%
	CTTCTTGTTGAAAGGTGGAA+AGG	+	chr2_3:55573243-55573262	None:intergenic	40.0%
	TTCCACCTTTCAACAAGAAG+TGG	-	chr2_3:55573243-55573262	Msa0300180:three_prime_UTR	40.0%
!	TCATGCCACTTCTTGTTGAA+AGG	+	chr2_3:55573251-55573270	None:intergenic	40.0%
	TTTCAACAAGAAGTGGCATG+AGG	-	chr2_3:55573250-55573269	Msa0300180:three_prime_UTR	40.0%
	TTCAACAAGAAGTGGCATGA+GGG	-	chr2_3:55573251-55573270	Msa0300180:three_prime_UTR	40.0%
	CCAAAAAAGAGAGACCAAGT+GGG	+	chr2_3:55573386-55573405	None:intergenic	40.0%
	ACCAAAAAAGAGAGACCAAG+TGG	+	chr2_3:55573387-55573406	None:intergenic	40.0%
	TATCTTTGCGTCTCTCCATA+AGG	+	chr2_3:55573508-55573527	None:intergenic	40.0%
	ATCAGAACTTGTCCTACATG+TGG	-	chr2_3:55573533-55573552	Msa0300180:CDS	40.0%
	GAATGTGAAGAACCTGATCT+TGG	+	chr2_3:55573574-55573593	None:intergenic	40.0%
!	GTTGTGTGTCATCGTGATTT+TGG	+	chr2_3:55573678-55573697	None:intergenic	40.0%
	AACATTTGGAGGCAAAAGTG+AGG	-	chr2_3:55573874-55573893	Msa0300180:CDS	40.0%
	CATTCTCTCCTTCAAGAGTA+GGG	+	chr2_3:55573941-55573960	None:intergenic	40.0%
	AATTGACCTGGTAACTTCTC+TGG	+	chr2_3:55573981-55574000	None:intergenic	40.0%
!	TTTGGTGGCTTAAATTGACC+TGG	+	chr2_3:55573993-55574012	None:intergenic	40.0%
	TACGTCTAATATGCAGGTGT+CGG	-	chr2_3:55574132-55574151	Msa0300180:intron	40.0%
	TGATCCGACATTTCTTTCAC+AGG	-	chr2_3:55574166-55574185	Msa0300180:intron	40.0%
	ATGGCCTGTGAAAGAAATGT+CGG	+	chr2_3:55574173-55574192	None:intergenic	40.0%
	GAGACAAGATGGCATAAAAC+AGG	-	chr2_3:55574255-55574274	Msa0300180:CDS	40.0%
	AACTTGCCACTCACGAAAAT+TGG	+	chr2_3:55574291-55574310	None:intergenic	40.0%
	TTCGTGAGTGGCAAGTTAAA+AGG	-	chr2_3:55574294-55574313	Msa0300180:CDS	40.0%
	GTTATGCACCAATACCATCT+TGG	-	chr2_3:55574384-55574403	Msa0300180:CDS	40.0%
	AGAGGAGAAAGAAGGAGAAT+TGG	-	chr2_3:55574416-55574435	Msa0300180:intron	40.0%
	AATGAACCACCACATTGTCT+AGG	+	chr2_3:55574471-55574490	None:intergenic	40.0%
	TGATCAAGGTGTGAATCATG+AGG	-	chr2_3:55574533-55574552	Msa0300180:intron	40.0%
!	TCTCATTTTCCTGTCCATGA+TGG	-	chr2_3:55574657-55574676	Msa0300180:CDS	40.0%
	AAAGATGTACCATCATGGAC+AGG	+	chr2_3:55574669-55574688	None:intergenic	40.0%
	CTAATGGTGAGGGATAAGTA+AGG	-	chr2_3:55574812-55574831	Msa0300180:five_prime_UTR	40.0%
	AGGCTTGATGCCTTTATACT+TGG	-	chr2_3:55574963-55574982	Msa0300180:five_prime_UTR	40.0%
	GGCTTGATGCCTTTATACTT+GGG	-	chr2_3:55574964-55574983	Msa0300180:five_prime_UTR	40.0%
	CTTCTTGTTGAAAGGTGGAA+AGG	+	chr2_3:55573243-55573262	None:intergenic	40.0%
	TTCCACCTTTCAACAAGAAG+TGG	-	chr2_3:55573243-55573262	Msa0300180:three_prime_UTR	40.0%
!	TCATGCCACTTCTTGTTGAA+AGG	+	chr2_3:55573251-55573270	None:intergenic	40.0%
	TTTCAACAAGAAGTGGCATG+AGG	-	chr2_3:55573250-55573269	Msa0300180:three_prime_UTR	40.0%
	TTCAACAAGAAGTGGCATGA+GGG	-	chr2_3:55573251-55573270	Msa0300180:three_prime_UTR	40.0%
	CCAAAAAAGAGAGACCAAGT+GGG	+	chr2_3:55573386-55573405	None:intergenic	40.0%
	ACCAAAAAAGAGAGACCAAG+TGG	+	chr2_3:55573387-55573406	None:intergenic	40.0%
	TATCTTTGCGTCTCTCCATA+AGG	+	chr2_3:55573508-55573527	None:intergenic	40.0%
	ATCAGAACTTGTCCTACATG+TGG	-	chr2_3:55573533-55573552	Msa0300180:CDS	40.0%
	GAATGTGAAGAACCTGATCT+TGG	+	chr2_3:55573574-55573593	None:intergenic	40.0%
!	GTTGTGTGTCATCGTGATTT+TGG	+	chr2_3:55573678-55573697	None:intergenic	40.0%
	AACATTTGGAGGCAAAAGTG+AGG	-	chr2_3:55573874-55573893	Msa0300180:CDS	40.0%
	CATTCTCTCCTTCAAGAGTA+GGG	+	chr2_3:55573941-55573960	None:intergenic	40.0%
	AATTGACCTGGTAACTTCTC+TGG	+	chr2_3:55573981-55574000	None:intergenic	40.0%
!	TTTGGTGGCTTAAATTGACC+TGG	+	chr2_3:55573993-55574012	None:intergenic	40.0%
	TACGTCTAATATGCAGGTGT+CGG	-	chr2_3:55574132-55574151	Msa0300180:intron	40.0%
	TGATCCGACATTTCTTTCAC+AGG	-	chr2_3:55574166-55574185	Msa0300180:intron	40.0%
	ATGGCCTGTGAAAGAAATGT+CGG	+	chr2_3:55574173-55574192	None:intergenic	40.0%
	GAGACAAGATGGCATAAAAC+AGG	-	chr2_3:55574255-55574274	Msa0300180:CDS	40.0%
	AACTTGCCACTCACGAAAAT+TGG	+	chr2_3:55574291-55574310	None:intergenic	40.0%
	TTCGTGAGTGGCAAGTTAAA+AGG	-	chr2_3:55574294-55574313	Msa0300180:CDS	40.0%
	GTTATGCACCAATACCATCT+TGG	-	chr2_3:55574384-55574403	Msa0300180:CDS	40.0%
	AGAGGAGAAAGAAGGAGAAT+TGG	-	chr2_3:55574416-55574435	Msa0300180:intron	40.0%
	AATGAACCACCACATTGTCT+AGG	+	chr2_3:55574471-55574490	None:intergenic	40.0%
	TGATCAAGGTGTGAATCATG+AGG	-	chr2_3:55574533-55574552	Msa0300180:intron	40.0%
!	TCTCATTTTCCTGTCCATGA+TGG	-	chr2_3:55574657-55574676	Msa0300180:CDS	40.0%
	AAAGATGTACCATCATGGAC+AGG	+	chr2_3:55574669-55574688	None:intergenic	40.0%
	CTAATGGTGAGGGATAAGTA+AGG	-	chr2_3:55574812-55574831	Msa0300180:five_prime_UTR	40.0%
	AGGCTTGATGCCTTTATACT+TGG	-	chr2_3:55574963-55574982	Msa0300180:five_prime_UTR	40.0%
	GGCTTGATGCCTTTATACTT+GGG	-	chr2_3:55574964-55574983	Msa0300180:five_prime_UTR	40.0%
!	TGCCACTTCTTGTTGAAAGG+TGG	+	chr2_3:55573248-55573267	None:intergenic	45.0%
	TCAACAAGAAGTGGCATGAG+GGG	-	chr2_3:55573252-55573271	Msa0300180:three_prime_UTR	45.0%
!!!	CCCACTTGGTCTCTCTTTTT+TGG	-	chr2_3:55573383-55573402	Msa0300180:three_prime_UTR	45.0%
	AGGTTCTTCACATTCGTGCA+TGG	-	chr2_3:55573579-55573598	Msa0300180:CDS	45.0%
	GCTGGAATTCATGAGTTACC+TGG	-	chr2_3:55573804-55573823	Msa0300180:CDS	45.0%
	AGTTACCTGGATTACCTGCT+GGG	-	chr2_3:55573817-55573836	Msa0300180:CDS	45.0%
	GAGTTCATCCCTACTCTTGA+AGG	-	chr2_3:55573930-55573949	Msa0300180:CDS	45.0%
	CCATTCTCTCCTTCAAGAGT+AGG	+	chr2_3:55573942-55573961	None:intergenic	45.0%
	CCTACTCTTGAAGGAGAGAA+TGG	-	chr2_3:55573939-55573958	Msa0300180:CDS	45.0%
	ACTCATCCAGAGAAGTTACC+AGG	-	chr2_3:55573972-55573991	Msa0300180:CDS	45.0%
	CCTGTTGTGTATGCTTTCGA+TGG	+	chr2_3:55574192-55574211	None:intergenic	45.0%
!	CCATCGAAAGCATACACAAC+AGG	-	chr2_3:55574189-55574208	Msa0300180:intron	45.0%
	AAAGTGCACACAGATGCAGA+TGG	-	chr2_3:55574228-55574247	Msa0300180:intron	45.0%
	CAGATGGAAGTGAGACAAGA+TGG	-	chr2_3:55574244-55574263	Msa0300180:CDS	45.0%
!	ACAAGGCCAATTTTCGTGAG+TGG	-	chr2_3:55574282-55574301	Msa0300180:CDS	45.0%
	CACATTGTCTAGGCTGTGTT+TGG	+	chr2_3:55574461-55574480	None:intergenic	45.0%
	CAAACACAGCCTAGACAATG+TGG	-	chr2_3:55574459-55574478	Msa0300180:intron	45.0%
	TCAAGGTGTGAATCATGAGG+TGG	-	chr2_3:55574536-55574555	Msa0300180:intron	45.0%
!	TGCCACTTCTTGTTGAAAGG+TGG	+	chr2_3:55573248-55573267	None:intergenic	45.0%
	TCAACAAGAAGTGGCATGAG+GGG	-	chr2_3:55573252-55573271	Msa0300180:three_prime_UTR	45.0%
!!!	CCCACTTGGTCTCTCTTTTT+TGG	-	chr2_3:55573383-55573402	Msa0300180:three_prime_UTR	45.0%
	AGGTTCTTCACATTCGTGCA+TGG	-	chr2_3:55573579-55573598	Msa0300180:CDS	45.0%
	GCTGGAATTCATGAGTTACC+TGG	-	chr2_3:55573804-55573823	Msa0300180:CDS	45.0%
	AGTTACCTGGATTACCTGCT+GGG	-	chr2_3:55573817-55573836	Msa0300180:CDS	45.0%
	GAGTTCATCCCTACTCTTGA+AGG	-	chr2_3:55573930-55573949	Msa0300180:CDS	45.0%
	CCATTCTCTCCTTCAAGAGT+AGG	+	chr2_3:55573942-55573961	None:intergenic	45.0%
	CCTACTCTTGAAGGAGAGAA+TGG	-	chr2_3:55573939-55573958	Msa0300180:CDS	45.0%
	ACTCATCCAGAGAAGTTACC+AGG	-	chr2_3:55573972-55573991	Msa0300180:CDS	45.0%
	CCTGTTGTGTATGCTTTCGA+TGG	+	chr2_3:55574192-55574211	None:intergenic	45.0%
!	CCATCGAAAGCATACACAAC+AGG	-	chr2_3:55574189-55574208	Msa0300180:intron	45.0%
	AAAGTGCACACAGATGCAGA+TGG	-	chr2_3:55574228-55574247	Msa0300180:intron	45.0%
	CAGATGGAAGTGAGACAAGA+TGG	-	chr2_3:55574244-55574263	Msa0300180:CDS	45.0%
!	ACAAGGCCAATTTTCGTGAG+TGG	-	chr2_3:55574282-55574301	Msa0300180:CDS	45.0%
	CACATTGTCTAGGCTGTGTT+TGG	+	chr2_3:55574461-55574480	None:intergenic	45.0%
	CAAACACAGCCTAGACAATG+TGG	-	chr2_3:55574459-55574478	Msa0300180:intron	45.0%
	TCAAGGTGTGAATCATGAGG+TGG	-	chr2_3:55574536-55574555	Msa0300180:intron	45.0%
	GAGTTACCTGGATTACCTGC+TGG	-	chr2_3:55573816-55573835	Msa0300180:CDS	50.0%
	GTTACCTGGATTACCTGCTG+GGG	-	chr2_3:55573818-55573837	Msa0300180:CDS	50.0%
!!	TATGCAGGTGTCGGCAAAGA+TGG	-	chr2_3:55574141-55574160	Msa0300180:intron	50.0%
	ACACAGCCTAGACAATGTGG+TGG	-	chr2_3:55574462-55574481	Msa0300180:intron	50.0%
	GAGTTACCTGGATTACCTGC+TGG	-	chr2_3:55573816-55573835	Msa0300180:CDS	50.0%
	GTTACCTGGATTACCTGCTG+GGG	-	chr2_3:55573818-55573837	Msa0300180:CDS	50.0%
!!	TATGCAGGTGTCGGCAAAGA+TGG	-	chr2_3:55574141-55574160	Msa0300180:intron	50.0%
	ACACAGCCTAGACAATGTGG+TGG	-	chr2_3:55574462-55574481	Msa0300180:intron	50.0%
	CTCTCTCTCTGTCTCCCACT+TGG	-	chr2_3:55573369-55573388	Msa0300180:three_prime_UTR	55.0%
	TTCACCCCAGCAGGTAATCC+AGG	+	chr2_3:55573825-55573844	None:intergenic	55.0%
	GGATCAAACTTCACCCCAGC+AGG	+	chr2_3:55573834-55573853	None:intergenic	55.0%
	CTCTCTCTCTGTCTCCCACT+TGG	-	chr2_3:55573369-55573388	Msa0300180:three_prime_UTR	55.0%
	TTCACCCCAGCAGGTAATCC+AGG	+	chr2_3:55573825-55573844	None:intergenic	55.0%
	GGATCAAACTTCACCCCAGC+AGG	+	chr2_3:55573834-55573853	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	55573137	55575091	55573137	ID=Msa0300180;Name=Msa0300180
chr2_3	mRNA	55573137	55575091	55573137	ID=Msa0300180-mRNA-1;Parent=Msa0300180;Name=Msa0300180-mRNA-1;_AED=0.07;_eAED=0.07;_QI=327\|1\|1\|1\|1\|1\|3\|393\|286
chr2_3	exon	55573137	55574080	55573137	ID=Msa0300180-mRNA-1:exon:17794;Parent=Msa0300180-mRNA-1
chr2_3	exon	55574235	55574427	55574235	ID=Msa0300180-mRNA-1:exon:17793;Parent=Msa0300180-mRNA-1
chr2_3	exon	55574648	55575091	55574648	ID=Msa0300180-mRNA-1:exon:17792;Parent=Msa0300180-mRNA-1
chr2_3	five_prime_UTR	55574765	55575091	55574765	ID=Msa0300180-mRNA-1:five_prime_utr;Parent=Msa0300180-mRNA-1
chr2_3	CDS	55574648	55574764	55574648	ID=Msa0300180-mRNA-1:cds;Parent=Msa0300180-mRNA-1
chr2_3	CDS	55574235	55574427	55574235	ID=Msa0300180-mRNA-1:cds;Parent=Msa0300180-mRNA-1
chr2_3	CDS	55573530	55574080	55573530	ID=Msa0300180-mRNA-1:cds;Parent=Msa0300180-mRNA-1
chr2_3	three_prime_UTR	55573137	55573529	55573137	ID=Msa0300180-mRNA-1:three_prime_utr;Parent=Msa0300180-mRNA-1

Gene Sequence

>Msa0300180

ATGACTCAGTGTAGTTACCCTGAAAATAATCATAGTACTATCCTTATGGAGAGACGCAAAGATAGCTTAATCAGAACTTGTCCTACATGTGGTCATCATATCAAATGCCAAGATCAGGGTGCTGGAATTCATGAGTTACCTGGATTACCTGCTGGGGTGAAGTTTGATCCTACTGATCAAGAGATTCTTGAACATTTGGAGGCAAAAGTGAGGTCTGATATTCAAATGCTTCATCCTTTAATTGATGAGTTCATCCCTACTCTTGAAGGAGAGAATGGAATCTGCTGTACTCATCCAGAGAAGTTACCAGGTGTCGGCAAAGATGGATTGATCCGACATTTCTTTCACAGGCCATCGAAAGCATACACAACAGGAACGAGAAAAAGAAGAAAAGTGCACACAGATGCAGATGGAAGTGAGACAAGATGGCATAAAACAGGTAAAACAAGGCCAATTTTCGTGAGTGGCAAGTTAAAAGGTTATAAGAAAATCCTTGTGCTTTACACTAACTACAGAAAGCAAAGAAAGCCAGAGAAAACAAATTGGGTTATGCACCAATACCATCTTGGAAATAATGAAGAGGAGAAAGAAGGAGAATTGGTAGTTTCCAAAGTTTTCTACCAAACACAGCCTAGACAATGTGGTGGTTCATTGATGAAAGACTCAGCATCATTTTCTGATAAAAAACTAAATGGTGATCAAGGTGTGAATCATGAGGTGGTTAATCATAAAAACAGTGGATTTGTTGAATACTATAGTACTTCTTTCATATCTTTTGATCAAGGTGAACAACATAGATCAAACAATGCTCAAGTGATTTCTCATTTTCCTGTCCATGATGGTACATCTTTCATTCCTTGA

Protein sequence

>Msa0300180

MTQCSYPENNHSTILMERRKDSLIRTCPTCGHHIKCQDQGAGIHELPGLPAGVKFDPTDQEILEHLEAKVRSDIQMLHPLIDEFIPTLEGENGICCTHPEKLPGVGKDGLIRHFFHRPSKAYTTGTRKRRKVHTDADGSETRWHKTGKTRPIFVSGKLKGYKKILVLYTNYRKQRKPEKTNWVMHQYHLGNNEEEKEGELVVSKVFYQTQPRQCGGSLMKDSASFSDKKLNGDQGVNHEVVNHKNSGFVEYYSTSFISFDQGEQHRSNNAQVISHFPVHDGTSFIP*