Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1261850 | XP_024638398.1 | 79.720 | 143 | 11 | 4 | 1 | 136 | 1 | 132 | 6.96e-54 | 192 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0046560 | Msa1261850 | 0.806319 | 8.713695e-50 | -8.615850e-47 |
| Msa0074750 | Msa1261850 | 0.805571 | 1.251883e-49 | -8.615850e-47 |
| Msa0081940 | Msa1261850 | 0.806608 | 7.572986e-50 | -8.615850e-47 |
| Msa0119440 | Msa1261850 | 0.818617 | 1.785306e-52 | -8.615850e-47 |
| Msa0640940 | Msa1261850 | 0.803724 | 3.040674e-49 | -8.615850e-47 |
| Msa0743530 | Msa1261850 | 0.819872 | 9.245902e-53 | -8.615850e-47 |
| Msa0789560 | Msa1261850 | 0.820426 | 6.901015e-53 | -8.615850e-47 |
| Msa0128430 | Msa1261850 | 0.813094 | 3.046490e-51 | -8.615850e-47 |
| Msa0146080 | Msa1261850 | 0.804631 | 1.969004e-49 | -8.615850e-47 |
| Msa0171980 | Msa1261850 | 0.815327 | 9.785132e-52 | -8.615850e-47 |
| Msa0192640 | Msa1261850 | 0.826152 | 3.173210e-54 | -8.615850e-47 |
| Msa0229360 | Msa1261850 | 0.801326 | 9.493674e-49 | -8.615850e-47 |
| Msa0238030 | Msa1261850 | 0.817532 | 3.140967e-52 | -8.615850e-47 |
| Msa1234620 | Msa1261850 | 0.809996 | 1.435330e-50 | -8.615850e-47 |
| Msa1242640 | Msa1261850 | 0.800910 | 1.154679e-48 | -8.615850e-47 |
| Msa1254620 | Msa1261850 | 0.807241 | 5.563632e-50 | -8.615850e-47 |
| Msa1261850 | Msa1297530 | 0.810007 | 1.427625e-50 | -8.615850e-47 |
| Msa1261850 | Msa1336040 | 0.805959 | 1.037556e-49 | -8.615850e-47 |
| Msa1261850 | Msa1344310 | 0.809485 | 1.848379e-50 | -8.615850e-47 |
| Msa1261850 | Msa1370590 | 0.803856 | 2.855308e-49 | -8.615850e-47 |
| Msa1261850 | Msa1384100 | 0.802112 | 6.546280e-49 | -8.615850e-47 |
| Msa1261850 | Msa1416260 | 0.819415 | 1.175401e-52 | -8.615850e-47 |
| Msa1261850 | Msa1421900 | 0.823471 | 1.360568e-53 | -8.615850e-47 |
| Msa1261850 | Msa1424350 | 0.817086 | 3.957407e-52 | -8.615850e-47 |
| Msa1261850 | Msa1426910 | 0.824042 | 9.998713e-54 | -8.615850e-47 |
| Msa1261850 | Msa1444120 | 0.801955 | 7.051608e-49 | -8.615850e-47 |
| Msa1261850 | Msa1454960 | 0.804766 | 1.845190e-49 | -8.615850e-47 |
| Msa0382370 | Msa1261850 | 0.802968 | 4.360887e-49 | -8.615850e-47 |
| Msa0472770 | Msa1261850 | 0.807194 | 5.693010e-50 | -8.615850e-47 |
| Msa1010460 | Msa1261850 | 0.820192 | 7.808868e-53 | -8.615850e-47 |
| Msa1063420 | Msa1261850 | 0.801476 | 8.843434e-49 | -8.615850e-47 |
| Msa0312910 | Msa1261850 | 0.818236 | 2.178124e-52 | -8.615850e-47 |
| Msa0321690 | Msa1261850 | 0.804515 | 2.082047e-49 | -8.615850e-47 |
| Msa0322900 | Msa1261850 | 0.815609 | 8.467585e-52 | -8.615850e-47 |
| Msa1127430 | Msa1261850 | 0.812869 | 3.412936e-51 | -8.615850e-47 |
| Msa1188560 | Msa1261850 | 0.805831 | 1.103718e-49 | -8.615850e-47 |
| Msa1205590 | Msa1261850 | 0.801285 | 9.678342e-49 | -8.615850e-47 |
| Msa1206650 | Msa1261850 | 0.810641 | 1.041847e-50 | -8.615850e-47 |
| Msa1208790 | Msa1261850 | 0.818606 | 1.795353e-52 | -8.615850e-47 |
| Msa1214320 | Msa1261850 | 0.805396 | 1.362247e-49 | -8.615850e-47 |
| Msa0482940 | Msa1261850 | 0.800094 | 1.693391e-48 | -8.615850e-47 |
| Msa0582280 | Msa1261850 | 0.817719 | 2.849894e-52 | -8.615850e-47 |
| Msa0583670 | Msa1261850 | 0.808274 | 3.356383e-50 | -8.615850e-47 |
| Msa0585070 | Msa1261850 | 0.806374 | 8.487033e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1261850 | MtrunA17_Chr4g0064311 | 79.720 | 143 | 11 | 4 | 1 | 136 | 1 | 132 | 6.40e-58 | 192 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1261850 | AT5G20250.4 | 43.902 | 82 | 33 | 1 | 55 | 136 | 62 | 130 | 5.46e-12 | 62.4 |
Find 24 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAGTGTGTACCAGAAATT+TGG | 0.279444 | 8_2:-64243254 | None:intergenic |
| ACCTTAAAAGAAGAAATTAA+TGG | 0.329731 | 8_2:-64243390 | None:intergenic |
| GGATTGGGTTTGATATTTAT+AGG | 0.341953 | 8_2:-64243132 | None:intergenic |
| TGTTTCCGACGGGAAACTAA+TGG | 0.384898 | 8_2:+64243657 | Msa1261850:CDS |
| TTTATAGGAAGAGAAGAAGA+AGG | 0.460246 | 8_2:-64243117 | None:intergenic |
| TGTTGTTGTTGGGAAAGAAA+TGG | 0.467610 | 8_2:-64243153 | None:intergenic |
| AAAGATCGGGCAATTCTAAC+CGG | 0.468947 | 8_2:+64243682 | Msa1261850:CDS |
| GGAATGTGTGATGTTGTTGT+TGG | 0.492962 | 8_2:-64243164 | None:intergenic |
| AGATATTTGAATAATACAGT+TGG | 0.497915 | 8_2:-64243213 | None:intergenic |
| GAAACTAATGGTAAAAGATC+GGG | 0.500863 | 8_2:+64243669 | Msa1261850:CDS |
| GGAAACTAATGGTAAAAGAT+CGG | 0.506270 | 8_2:+64243668 | Msa1261850:CDS |
| CCGTCGGAAACACGAACAGC+GGG | 0.507750 | 8_2:-64243646 | None:intergenic |
| AGTTACGTTGGCGGATACTC+CGG | 0.517952 | 8_2:-64243701 | None:intergenic |
| TTGTTGGGAAAGAAATGGAT+TGG | 0.521258 | 8_2:-64243148 | None:intergenic |
| TGTTGGGAAAGAAATGGATT+GGG | 0.526616 | 8_2:-64243147 | None:intergenic |
| CCCGCTGTTCGTGTTTCCGA+CGG | 0.540092 | 8_2:+64243646 | Msa1261850:CDS |
| GAATGTGTGATGTTGTTGTT+GGG | 0.543302 | 8_2:-64243163 | None:intergenic |
| CCGCTGTTCGTGTTTCCGAC+GGG | 0.549298 | 8_2:+64243647 | Msa1261850:CDS |
| TGACGTCTCAGTTACGTTGG+CGG | 0.579093 | 8_2:-64243710 | None:intergenic |
| TATGACTATGAATACGTGAA+AGG | 0.580032 | 8_2:-64243185 | None:intergenic |
| GTTGTAGAGAAGAGAGGTTG+AGG | 0.603393 | 8_2:+64243600 | Msa1261850:intron |
| TTATCCGTTGTAGAGAAGAG+AGG | 0.618799 | 8_2:+64243594 | Msa1261850:intron |
| GAGAAACATGTTAACAACAA+TGG | 0.640489 | 8_2:+64243071 | Msa1261850:CDS |
| CCCGTCGGAAACACGAACAG+CGG | 0.644746 | 8_2:-64243647 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTATGAATAAAAAGTAAA+AGG | - | chr8_2:64243319-64243338 | None:intergenic | 15.0% |
| !! | ATATAAAATAATGAAAATGG+AGG | - | chr8_2:64243564-64243583 | None:intergenic | 15.0% |
| !! | AGATATTTGAATAATACAGT+TGG | - | chr8_2:64243216-64243235 | None:intergenic | 20.0% |
| !!! | TCCATTAATTTCTTCTTTTA+AGG | + | chr8_2:64243389-64243408 | Msa1261850:CDS | 20.0% |
| !! | ACCTTAAAAGAAGAAATTAA+TGG | - | chr8_2:64243393-64243412 | None:intergenic | 20.0% |
| !! | AAATTTGGAGATGATTTTTG+TGG | - | chr8_2:64243242-64243261 | None:intergenic | 25.0% |
| ! | AAAAATCATCTCCAAATTTC+TGG | + | chr8_2:64243243-64243262 | Msa1261850:CDS | 25.0% |
| ! | AAGGAACAAAAAAGAATTAC+AGG | - | chr8_2:64243300-64243319 | None:intergenic | 25.0% |
| ! | TGATATCACAATGAATATGA+CGG | + | chr8_2:64243456-64243475 | Msa1261850:intron | 25.0% |
| GAGAAACATGTTAACAACAA+TGG | + | chr8_2:64243071-64243090 | Msa1261850:CDS | 30.0% | |
| TTTATAGGAAGAGAAGAAGA+AGG | - | chr8_2:64243120-64243139 | None:intergenic | 30.0% | |
| GGATTGGGTTTGATATTTAT+AGG | - | chr8_2:64243135-64243154 | None:intergenic | 30.0% | |
| TATGACTATGAATACGTGAA+AGG | - | chr8_2:64243188-64243207 | None:intergenic | 30.0% | |
| GGAAACTAATGGTAAAAGAT+CGG | + | chr8_2:64243668-64243687 | Msa1261850:CDS | 30.0% | |
| GAAACTAATGGTAAAAGATC+GGG | + | chr8_2:64243669-64243688 | Msa1261850:CDS | 30.0% | |
| ! | TGTTGGGAAAGAAATGGATT+GGG | - | chr8_2:64243150-64243169 | None:intergenic | 35.0% |
| TTGTTGGGAAAGAAATGGAT+TGG | - | chr8_2:64243151-64243170 | None:intergenic | 35.0% | |
| ! | TGTTGTTGTTGGGAAAGAAA+TGG | - | chr8_2:64243156-64243175 | None:intergenic | 35.0% |
| ! | GAATGTGTGATGTTGTTGTT+GGG | - | chr8_2:64243166-64243185 | None:intergenic | 35.0% |
| ! | AAGAGTGTGTACCAGAAATT+TGG | - | chr8_2:64243257-64243276 | None:intergenic | 35.0% |
| !! | TTTTACCATTAGTTTCCCGT+CGG | - | chr8_2:64243665-64243684 | None:intergenic | 35.0% |
| GGAATGTGTGATGTTGTTGT+TGG | - | chr8_2:64243167-64243186 | None:intergenic | 40.0% | |
| TTATCCGTTGTAGAGAAGAG+AGG | + | chr8_2:64243594-64243613 | Msa1261850:intron | 40.0% | |
| TCAACCTCTCTTCTCTACAA+CGG | - | chr8_2:64243601-64243620 | None:intergenic | 40.0% | |
| AAAGATCGGGCAATTCTAAC+CGG | + | chr8_2:64243682-64243701 | Msa1261850:CDS | 40.0% | |
| GTTGTAGAGAAGAGAGGTTG+AGG | + | chr8_2:64243600-64243619 | Msa1261850:intron | 45.0% | |
| TGTTTCCGACGGGAAACTAA+TGG | + | chr8_2:64243657-64243676 | Msa1261850:CDS | 45.0% | |
| !! | TAAATATAAAATAATGAAAA+TGG | - | chr8_2:64243567-64243586 | None:intergenic | 5.0% |
| ! | AGTTACGTTGGCGGATACTC+CGG | - | chr8_2:64243704-64243723 | None:intergenic | 50.0% |
| TGACGTCTCAGTTACGTTGG+CGG | - | chr8_2:64243713-64243732 | None:intergenic | 50.0% | |
| TGCTGACGTCTCAGTTACGT+TGG | - | chr8_2:64243716-64243735 | None:intergenic | 50.0% | |
| CCGTCGGAAACACGAACAGC+GGG | - | chr8_2:64243649-64243668 | None:intergenic | 60.0% | |
| CCCGCTGTTCGTGTTTCCGA+CGG | + | chr8_2:64243646-64243665 | Msa1261850:CDS | 60.0% | |
| CCCGTCGGAAACACGAACAG+CGG | - | chr8_2:64243650-64243669 | None:intergenic | 60.0% | |
| CCGCTGTTCGTGTTTCCGAC+GGG | + | chr8_2:64243647-64243666 | Msa1261850:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 64243026 | 64243738 | 64243026 | ID=Msa1261850;Name=Msa1261850 |
| chr8_2 | mRNA | 64243026 | 64243738 | 64243026 | ID=Msa1261850-mRNA-1;Parent=Msa1261850;Name=Msa1261850-mRNA-1;_AED=0.10;_eAED=0.10;_QI=16|1|0.66|1|1|1|3|0|136 |
| chr8_2 | exon | 64243026 | 64243264 | 64243026 | ID=Msa1261850-mRNA-1:exon:20798;Parent=Msa1261850-mRNA-1 |
| chr8_2 | exon | 64243358 | 64243410 | 64243358 | ID=Msa1261850-mRNA-1:exon:20799;Parent=Msa1261850-mRNA-1 |
| chr8_2 | exon | 64243607 | 64243738 | 64243607 | ID=Msa1261850-mRNA-1:exon:20800;Parent=Msa1261850-mRNA-1 |
| chr8_2 | five_prime_UTR | 64243026 | 64243041 | 64243026 | ID=Msa1261850-mRNA-1:five_prime_utr;Parent=Msa1261850-mRNA-1 |
| chr8_2 | CDS | 64243042 | 64243264 | 64243042 | ID=Msa1261850-mRNA-1:cds;Parent=Msa1261850-mRNA-1 |
| chr8_2 | CDS | 64243358 | 64243410 | 64243358 | ID=Msa1261850-mRNA-1:cds;Parent=Msa1261850-mRNA-1 |
| chr8_2 | CDS | 64243607 | 64243738 | 64243607 | ID=Msa1261850-mRNA-1:cds;Parent=Msa1261850-mRNA-1 |
| Gene Sequence |
| Protein sequence |
