AlfalfaGEDB | Msa1261850

M. sativa cultivar zhongmu-4 / Msa1261850

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261850	XP_024638398.1	79.720	143	11	4	1	136	1	132	6.96e-54	192

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0046560	Msa1261850	0.806319	8.713695e-50	-8.615850e-47
Msa0074750	Msa1261850	0.805571	1.251883e-49	-8.615850e-47
Msa0081940	Msa1261850	0.806608	7.572986e-50	-8.615850e-47
Msa0119440	Msa1261850	0.818617	1.785306e-52	-8.615850e-47
Msa0128430	Msa1261850	0.813094	3.046490e-51	-8.615850e-47
Msa0146080	Msa1261850	0.804631	1.969004e-49	-8.615850e-47
Msa0171980	Msa1261850	0.815327	9.785132e-52	-8.615850e-47
Msa0192640	Msa1261850	0.826152	3.173210e-54	-8.615850e-47
Msa0229360	Msa1261850	0.801326	9.493674e-49	-8.615850e-47
Msa0238030	Msa1261850	0.817532	3.140967e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261850	MtrunA17_Chr4g0064311	79.720	143	11	4	1	136	1	132	6.40e-58	192

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261850	AT5G20250.4	43.902	82	33	1	55	136	62	130	5.46e-12	62.4

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Find 24 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGATCGGGCAATTCTAAC+CGG	0.468947	8_2:+64243682	Msa1261850:CDS
AAGAGTGTGTACCAGAAATT+TGG	0.279444	8_2:-64243254	None:intergenic
ACCTTAAAAGAAGAAATTAA+TGG	0.329731	8_2:-64243390	None:intergenic
AGATATTTGAATAATACAGT+TGG	0.497915	8_2:-64243213	None:intergenic
AGTTACGTTGGCGGATACTC+CGG	0.517952	8_2:-64243701	None:intergenic
CCCGCTGTTCGTGTTTCCGA+CGG	0.540092	8_2:+64243646	Msa1261850:CDS
CCCGTCGGAAACACGAACAG+CGG	0.644746	8_2:-64243647	None:intergenic
CCGCTGTTCGTGTTTCCGAC+GGG	0.549298	8_2:+64243647	Msa1261850:CDS
CCGTCGGAAACACGAACAGC+GGG	0.507750	8_2:-64243646	None:intergenic
GAAACTAATGGTAAAAGATC+GGG	0.500863	8_2:+64243669	Msa1261850:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAGAAACATGTTAACAACAA+TGG	+	chr8_2:64243071-64243090	Msa1261850:CDS	30.0%
TTTATAGGAAGAGAAGAAGA+AGG	-	chr8_2:64243120-64243139	None:intergenic	30.0%
GGATTGGGTTTGATATTTAT+AGG	-	chr8_2:64243135-64243154	None:intergenic	30.0%
TATGACTATGAATACGTGAA+AGG	-	chr8_2:64243188-64243207	None:intergenic	30.0%
GGAAACTAATGGTAAAAGAT+CGG	+	chr8_2:64243668-64243687	Msa1261850:CDS	30.0%
GAAACTAATGGTAAAAGATC+GGG	+	chr8_2:64243669-64243688	Msa1261850:CDS	30.0%
TTGTTGGGAAAGAAATGGAT+TGG	-	chr8_2:64243151-64243170	None:intergenic	35.0%
GGAATGTGTGATGTTGTTGT+TGG	-	chr8_2:64243167-64243186	None:intergenic	40.0%
TTATCCGTTGTAGAGAAGAG+AGG	+	chr8_2:64243594-64243613	Msa1261850:intron	40.0%
TCAACCTCTCTTCTCTACAA+CGG	-	chr8_2:64243601-64243620	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	64243026	64243738	64243026	ID=Msa1261850;Name=Msa1261850
chr8_2	mRNA	64243026	64243738	64243026	ID=Msa1261850-mRNA-1;Parent=Msa1261850;Name=Msa1261850-mRNA-1;_AED=0.10;_eAED=0.10;_QI=16\|1\|0.66\|1\|1\|1\|3\|0\|136
chr8_2	exon	64243026	64243264	64243026	ID=Msa1261850-mRNA-1:exon:20798;Parent=Msa1261850-mRNA-1
chr8_2	exon	64243358	64243410	64243358	ID=Msa1261850-mRNA-1:exon:20799;Parent=Msa1261850-mRNA-1
chr8_2	exon	64243607	64243738	64243607	ID=Msa1261850-mRNA-1:exon:20800;Parent=Msa1261850-mRNA-1
chr8_2	five_prime_UTR	64243026	64243041	64243026	ID=Msa1261850-mRNA-1:five_prime_utr;Parent=Msa1261850-mRNA-1
chr8_2	CDS	64243042	64243264	64243042	ID=Msa1261850-mRNA-1:cds;Parent=Msa1261850-mRNA-1
chr8_2	CDS	64243358	64243410	64243358	ID=Msa1261850-mRNA-1:cds;Parent=Msa1261850-mRNA-1
chr8_2	CDS	64243607	64243738	64243607	ID=Msa1261850-mRNA-1:cds;Parent=Msa1261850-mRNA-1

Gene Sequence

>Msa1261850

ATGATAGTTCTTGTTAACAACACTTTGACGAGAAACATGTTAACAACAATGGTGTTCAACTCATGTTATTATAATCCTTCTTCTTCTCTTCCTATAAATATCAAACCCAATCCATTTCTTTCCCAACAACAACATCACACATTCCTTTCACGTATTCATAGTCATAATAATCCAACTGTATTATTCAAATATCTCAACCACAAAAATCATCTCCAAATTTCTGATTTTACAAAACACTATTCAAATACAACATTTCCATTAATTTCTTCTTTTAAGAGAAGAGAGGTTGAGGTTGAAGAAGAAATGACGATCAAACCCGCTGTTCGTGTTTCCGACGGGAAACTAATGGTAAAAGATCGGGCAATTCTAACCGGAGTATCCGCCAACGTAACTGAGACGTCAGCAGCT

Protein sequence

>Msa1261850

MIVLVNNTLTRNMLTTMVFNSCYYNPSSSLPINIKPNPFLSQQQHHTFLSRIHSHNNPTVLFKYLNHKNHLQISDFTKHYSNTTFPLISSFKRREVEVEEEMTIKPAVRVSDGKLMVKDRAILTGVSANVTETSAA