AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1206650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1206650 MtrunA17_Chr4g0055651 100.000 197 0 0 11 207 6 202 8.72e-148 409
Msa1206650 MtrunA17_Chr7g0250531 87.817 197 24 0 11 207 6 202 2.90e-132 369
Msa1206650 MtrunA17_Chr8g0335471 87.879 198 23 1 11 207 6 203 5.21e-130 363
Msa1206650 MtrunA17_Chr2g0276931 77.551 196 43 1 11 205 7 202 2.58e-114 324
Msa1206650 MtrunA17_Chr4g0030911 60.099 203 71 4 11 205 13 213 5.73e-85 250
Msa1206650 MtrunA17_Chr1g0212521 58.621 203 75 3 11 205 13 214 5.28e-84 248
Msa1206650 MtrunA17_Chr1g0182321 58.738 206 74 4 11 207 13 216 5.58e-84 248
Msa1206650 MtrunA17_Chr7g0263071 59.223 206 73 4 11 207 13 216 1.32e-83 247
Msa1206650 MtrunA17_Chr1g0182301 58.252 206 75 4 11 207 13 216 2.41e-83 246
Msa1206650 MtrunA17_Chr2g0327911 52.427 206 81 5 11 205 13 212 8.18e-71 214
Msa1206650 MtrunA17_Chr4g0032201 46.602 206 101 2 11 207 10 215 4.90e-64 197
Msa1206650 MtrunA17_Chr2g0276941 47.115 208 99 3 11 207 13 220 1.17e-61 191
Msa1206650 MtrunA17_Chr2g0290891 45.411 207 101 1 12 206 5 211 5.27e-61 189
Msa1206650 MtrunA17_Chr6g0458081 54.268 164 75 0 11 174 4 167 8.60e-61 189
Msa1206650 MtrunA17_Chr2g0315551 46.860 207 100 2 11 207 11 217 1.68e-59 186
Msa1206650 MtrunA17_Chr7g0234201 47.059 204 99 1 12 206 12 215 4.96e-59 184
Msa1206650 MtrunA17_Chr1g0189361 46.698 212 97 3 11 206 12 223 5.15e-59 184
Msa1206650 MtrunA17_Chr3g0112681 45.714 210 98 3 11 207 10 216 6.09e-59 184
Msa1206650 MtrunA17_Chr8g0384311 48.241 199 98 4 11 205 11 208 1.59e-58 183
Msa1206650 MtrunA17_Chr3g0123821 44.231 208 103 2 11 205 14 221 1.60e-58 183
Msa1206650 MtrunA17_Chr8g0381841 53.293 167 76 2 11 176 15 180 2.26e-58 183
Msa1206650 MtrunA17_Chr7g0265001 47.761 201 100 3 11 206 14 214 2.93e-58 182
Msa1206650 MtrunA17_Chr2g0283131 45.411 207 103 1 11 207 11 217 5.72e-58 182
Msa1206650 MtrunA17_Chr4g0046261 45.411 207 103 1 11 207 11 217 5.97e-58 182
Msa1206650 MtrunA17_Chr8g0349711 51.534 163 79 0 12 174 5 167 2.20e-57 180
Msa1206650 MtrunA17_Chr4g0040781 44.928 207 104 1 11 207 11 217 4.93e-57 179
Msa1206650 MtrunA17_Chr2g0325091 45.631 206 103 1 11 207 9 214 5.08e-56 177
Msa1206650 MtrunA17_Chr1g0159481 48.756 201 97 4 11 206 15 214 1.16e-55 176
Msa1206650 MtrunA17_Chr8g0349681 52.695 167 79 0 12 178 383 549 1.19e-55 186
Msa1206650 MtrunA17_Chr8g0349681 49.080 163 83 0 12 174 174 336 1.92e-50 172
Msa1206650 MtrunA17_Chr8g0349681 50.000 160 80 0 12 171 12 171 6.00e-50 170
Msa1206650 MtrunA17_Chr2g0319951 45.933 209 100 3 11 207 11 218 1.26e-55 176
Msa1206650 MtrunA17_Chr8g0346421 44.041 193 108 0 11 203 24 216 1.34e-55 176
Msa1206650 MtrunA17_Chr7g0275991 43.878 196 106 2 11 203 18 212 8.76e-55 174
Msa1206650 MtrunA17_Chr1g0180291 47.000 200 100 5 11 205 12 210 1.34e-54 173
Msa1206650 MtrunA17_Chr7g0231131 43.478 207 105 2 11 205 13 219 3.58e-54 172
Msa1206650 MtrunA17_Chr6g0482721 50.276 181 88 2 11 190 10 189 4.28e-54 172
Msa1206650 MtrunA17_Chr8g0351861 47.368 171 90 0 11 181 14 184 1.12e-53 171
Msa1206650 MtrunA17_Chr3g0130271 46.000 200 102 5 11 205 10 208 2.92e-53 169
Msa1206650 MtrunA17_Chr1g0174281 49.390 164 83 0 11 174 13 176 6.21e-53 169
Msa1206650 MtrunA17_Chr8g0392931 47.596 208 96 4 11 206 12 218 1.12e-52 168
Msa1206650 MtrunA17_Chr7g0214241 47.126 174 92 0 12 185 14 187 3.48e-52 167
Msa1206650 MtrunA17_Chr1g0197331 47.500 200 101 4 11 206 13 212 5.56e-52 166
Msa1206650 MtrunA17_Chr3g0109721 44.103 195 100 4 15 206 12 200 6.24e-52 166
Msa1206650 MtrunA17_Chr5g0433561 42.268 194 105 3 15 206 12 200 2.35e-50 162
Msa1206650 MtrunA17_Chr8g0390561 41.237 194 106 3 15 206 11 198 8.99e-48 155
Msa1206650 MtrunA17_Chr1g0199331 42.236 161 92 1 15 174 33 193 2.91e-43 144
Msa1206650 MtrunA17_Chr8g0382221 37.056 197 120 1 14 206 10 206 7.55e-43 143
Msa1206650 MtrunA17_Chr5g0400131 36.735 196 119 1 14 204 10 205 2.11e-42 142
Msa1206650 MtrunA17_Chr4g0055081 36.041 197 118 2 14 204 10 204 1.64e-41 139
Msa1206650 MtrunA17_Chr3g0123971 41.615 161 94 0 14 174 10 170 2.63e-41 139
Msa1206650 MtrunA17_Chr2g0331691 38.308 201 113 5 13 205 10 207 6.42e-40 135
Msa1206650 MtrunA17_Chr4g0004661 38.308 201 113 5 13 205 64 261 6.64e-39 134
Msa1206650 MtrunA17_Chr4g0034871 40.476 168 93 3 13 173 8 175 5.56e-38 130
Msa1206650 MtrunA17_Chr6g0466301 35.922 206 112 6 13 206 8 205 3.64e-35 123
Msa1206650 MtrunA17_Chr6g0465491 35.922 206 112 6 13 206 8 205 3.64e-35 123
Msa1206650 MtrunA17_Chr1g0201121 37.952 166 96 3 13 171 8 173 6.39e-34 120
Msa1206650 MtrunA17_Chr8g0383261 36.747 166 98 2 13 171 12 177 7.15e-33 117
Msa1206650 MtrunA17_Chr3g0138121 30.435 184 119 4 14 193 14 192 3.38e-24 95.5
Msa1206650 MtrunA17_Chr3g0138101 30.435 184 119 4 14 193 14 192 3.42e-24 95.5
Msa1206650 MtrunA17_Chr3g0138111 30.435 184 119 4 14 193 14 192 3.88e-24 95.1
Msa1206650 MtrunA17_Chr6g0465471 33.690 187 104 6 30 204 7 185 8.41e-24 94.0
Msa1206650 MtrunA17_Chr6g0466161 33.690 187 104 6 30 204 7 185 1.27e-23 93.6
Msa1206650 MtrunA17_Chr4g0037021 31.737 167 100 5 13 167 6 170 7.11e-23 91.3
Msa1206650 MtrunA17_Chr6g0486041 31.737 167 100 5 13 167 6 170 7.34e-23 91.7
Msa1206650 MtrunA17_Chr8g0371501 31.953 169 101 5 13 169 6 172 1.75e-22 90.1
Msa1206650 MtrunA17_Chr4g0046211 35.772 123 76 3 13 134 6 126 3.05e-22 88.2
Msa1206650 MtrunA17_Chr5g0405791 31.361 169 102 5 13 169 6 172 9.46e-22 88.2
Msa1206650 MtrunA17_Chr1g0178291 35.928 167 100 3 16 182 431 590 3.86e-21 90.9
Msa1206650 MtrunA17_Chr3g0117931 30.055 183 109 6 13 183 8 183 4.10e-21 87.0
Msa1206650 MtrunA17_Chr2g0325101 29.586 169 105 5 13 169 6 172 4.50e-21 86.7
Msa1206650 MtrunA17_Chr6g0466431 35.354 99 55 1 13 111 8 97 6.73e-17 72.8
Msa1206650 MtrunA17_Chr6g0465611 35.354 99 55 1 13 111 8 97 6.73e-17 72.8
Msa1206650 MtrunA17_Chr5g0434561 36.232 138 78 4 14 150 20 148 3.57e-15 70.5
Msa1206650 MtrunA17_Chr5g0401071 28.994 169 110 5 14 177 20 183 1.18e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1206650 AT5G47200.1 89.848 197 20 0 11 207 6 202 1.47e-134 375
Msa1206650 AT4G17530.1 89.340 197 21 0 11 207 6 202 8.91e-134 374
Msa1206650 AT1G02130.1 86.869 198 25 1 11 207 6 203 9.78e-130 363
Msa1206650 AT3G11730.1 75.758 198 45 3 11 205 6 203 1.84e-111 317
Msa1206650 AT5G59840.1 59.709 206 72 4 11 207 13 216 4.84e-85 251
Msa1206650 AT3G46060.2 58.738 206 74 4 11 207 13 216 9.79e-84 248
Msa1206650 AT3G46060.3 58.738 206 74 4 11 207 13 216 9.79e-84 248
Msa1206650 AT3G46060.1 58.738 206 74 4 11 207 13 216 9.79e-84 248
Msa1206650 AT3G53610.2 57.561 205 78 3 11 207 13 216 9.42e-83 245
Msa1206650 AT3G53610.3 57.561 205 78 3 11 207 13 216 9.42e-83 245
Msa1206650 AT3G53610.1 57.561 205 78 3 11 207 13 216 9.42e-83 245
Msa1206650 AT3G09900.1 58.537 205 75 3 11 206 13 216 1.57e-80 239
Msa1206650 AT5G03520.1 59.113 203 75 3 11 206 13 214 7.17e-80 238
Msa1206650 AT4G17160.1 48.756 201 95 2 12 205 5 204 9.11e-65 199
Msa1206650 AT5G47520.1 49.048 210 94 2 11 207 12 221 1.39e-63 196
Msa1206650 AT1G07410.1 45.854 205 103 2 11 207 10 214 3.24e-62 193
Msa1206650 AT4G35860.1 45.116 215 92 2 11 206 4 211 3.29e-62 192
Msa1206650 AT4G17170.1 46.377 207 99 2 12 206 5 211 2.64e-61 190
Msa1206650 AT1G09630.1 47.115 208 98 3 11 207 10 216 1.34e-60 189
Msa1206650 AT5G03520.2 54.819 166 67 3 48 206 40 204 1.42e-60 188
Msa1206650 AT4G39990.1 46.190 210 99 4 11 207 15 223 3.72e-60 188
Msa1206650 AT3G07410.1 48.309 207 96 3 11 206 10 216 5.70e-60 187
Msa1206650 AT5G45750.1 47.573 206 99 2 11 207 11 216 5.84e-60 187
Msa1206650 AT3G15060.1 48.558 208 95 3 11 207 11 217 2.04e-59 186
Msa1206650 AT1G28550.1 47.368 209 97 3 11 207 11 218 5.02e-59 185
Msa1206650 AT1G16920.1 47.573 206 99 2 11 207 11 216 6.13e-59 184
Msa1206650 AT4G18430.1 46.117 206 101 1 11 206 11 216 6.39e-59 184
Msa1206650 AT5G65270.1 45.540 213 99 5 11 207 15 226 7.00e-59 185
Msa1206650 AT3G46830.1 44.231 208 105 1 11 207 10 217 9.25e-59 184
Msa1206650 AT3G46830.2 44.231 208 105 1 11 207 10 217 9.25e-59 184
Msa1206650 AT5G59150.1 43.269 208 107 1 11 207 10 217 1.18e-58 184
Msa1206650 AT5G47960.1 44.762 210 103 3 11 207 13 222 2.14e-58 183
Msa1206650 AT5G60860.1 45.894 207 102 1 11 207 11 217 2.14e-58 183
Msa1206650 AT2G33870.1 46.890 209 98 3 11 207 11 218 3.16e-58 183
Msa1206650 AT1G06400.1 45.146 206 104 2 11 207 11 216 4.69e-58 182
Msa1206650 AT4G18800.1 46.078 204 103 2 11 207 11 214 2.36e-57 180
Msa1206650 AT1G05810.1 44.231 208 105 2 11 207 10 217 7.68e-56 177
Msa1206650 AT1G05810.2 44.231 208 105 2 11 207 56 263 1.92e-55 177
Msa1206650 AT2G43130.1 44.608 204 105 2 11 206 10 213 4.04e-55 175
Msa1206650 AT1G18200.1 47.236 199 95 1 11 199 11 209 2.24e-54 173
Msa1206650 AT5G03530.2 48.259 201 98 4 11 206 11 210 2.71e-54 172
Msa1206650 AT5G03530.1 48.259 201 98 4 11 206 11 210 2.71e-54 172
Msa1206650 AT3G12160.1 43.269 208 104 3 11 205 13 219 3.85e-54 172
Msa1206650 AT2G31680.1 43.062 209 107 2 11 207 10 218 1.79e-53 171
Msa1206650 AT1G73640.1 48.677 189 92 2 11 195 11 198 3.10e-53 171
Msa1206650 AT1G43890.3 54.217 166 73 3 11 174 11 175 3.54e-53 170
Msa1206650 AT1G43890.1 54.217 166 73 3 11 174 11 175 3.54e-53 170
Msa1206650 AT1G43890.2 54.217 166 73 3 11 174 11 175 3.54e-53 170
Msa1206650 AT1G01200.1 44.388 196 106 2 11 203 26 221 1.07e-50 164
Msa1206650 AT4G19640.1 41.969 193 107 2 15 206 12 200 2.37e-48 157
Msa1206650 AT5G45130.1 41.538 195 105 4 15 206 12 200 4.88e-46 151
Msa1206650 AT3G54840.1 43.353 173 92 3 15 186 35 202 1.12e-44 147
Msa1206650 AT5G45130.2 40.933 193 105 4 17 206 22 208 1.47e-44 147
Msa1206650 AT3G54840.3 43.023 172 92 3 16 186 2 168 2.03e-44 146
Msa1206650 AT2G44610.1 37.879 198 118 1 14 206 10 207 2.51e-44 147
Msa1206650 AT3G54840.2 44.805 154 84 1 15 167 35 188 6.15e-44 145
Msa1206650 AT3G09910.5 45.109 184 97 4 11 192 11 192 1.64e-43 144
Msa1206650 AT3G09910.4 45.109 184 97 4 11 192 11 192 1.64e-43 144
Msa1206650 AT3G09910.3 44.221 199 105 4 11 207 11 205 3.19e-43 144
Msa1206650 AT3G09910.1 44.221 199 105 4 11 207 11 205 3.19e-43 144
Msa1206650 AT3G09910.2 44.221 199 105 4 11 207 11 205 3.19e-43 144
Msa1206650 AT4G35860.2 53.333 120 56 0 55 174 2 121 1.93e-42 140
Msa1206650 AT2G22290.1 38.191 199 115 3 14 206 10 206 7.71e-42 140
Msa1206650 AT5G10260.1 36.041 197 121 1 14 205 10 206 7.30e-41 138
Msa1206650 AT1G52280.1 39.500 200 110 5 13 204 8 204 1.01e-40 137
Msa1206650 AT4G39890.1 41.975 162 93 1 14 174 10 171 1.35e-40 137
Msa1206650 AT5G59150.2 39.634 164 88 1 55 207 3 166 2.95e-40 135
Msa1206650 AT3G18820.1 42.857 168 89 3 13 173 8 175 3.59e-40 136
Msa1206650 AT3G16100.1 38.500 200 112 5 13 204 8 204 1.66e-39 134
Msa1206650 AT5G64990.1 39.759 166 100 0 14 179 8 173 2.97e-38 131
Msa1206650 AT4G19640.3 45.802 131 71 0 15 145 12 142 2.45e-37 129
Msa1206650 AT1G49300.2 37.000 200 115 5 13 204 8 204 4.69e-37 128
Msa1206650 AT1G49300.1 37.000 200 115 5 13 204 8 204 4.69e-37 128
Msa1206650 AT2G21880.1 39.157 166 94 3 13 171 9 174 2.24e-36 127
Msa1206650 AT4G19640.2 46.400 125 67 0 15 139 12 136 2.42e-36 125
Msa1206650 AT5G64990.2 38.150 173 100 1 14 179 8 180 1.79e-35 124
Msa1206650 AT1G22740.1 35.784 204 109 5 13 204 8 201 1.82e-35 124
Msa1206650 AT4G09720.1 39.759 166 93 3 13 171 8 173 8.97e-34 120
Msa1206650 AT4G09720.4 37.427 171 95 3 13 171 8 178 9.06e-32 115
Msa1206650 AT5G39620.1 40.000 165 92 4 14 171 6 170 2.44e-31 114
Msa1206650 AT4G09720.3 36.158 177 95 3 13 171 8 184 9.96e-31 112
Msa1206650 AT2G21880.4 40.146 137 75 3 42 171 13 149 1.65e-28 105
Msa1206650 AT2G21880.3 40.146 137 75 3 42 171 13 149 1.65e-28 105
Msa1206650 AT2G21880.2 34.337 166 94 4 13 171 9 166 1.76e-26 101
Msa1206650 AT4G09720.6 40.876 137 74 3 42 171 3 139 3.71e-26 99.4
Msa1206650 AT4G09720.5 40.876 137 74 3 42 171 3 139 3.71e-26 99.4
Msa1206650 AT4G09720.2 40.876 137 74 3 42 171 3 139 3.71e-26 99.4
Msa1206650 AT2G44690.1 34.132 167 96 5 13 167 18 182 4.09e-25 97.8
Msa1206650 AT4G35020.1 33.728 169 98 5 13 169 6 172 1.10e-24 96.3
Msa1206650 AT4G35020.2 33.728 169 98 5 13 169 6 172 1.10e-24 96.3
Msa1206650 AT4G35020.3 33.728 169 98 5 13 169 6 172 1.10e-24 96.3
Msa1206650 AT4G28950.1 33.333 165 98 5 13 167 6 168 3.06e-24 95.5
Msa1206650 AT5G55080.1 30.864 162 107 4 14 174 14 171 3.40e-24 95.9
Msa1206650 AT5G55190.1 30.435 184 119 4 14 193 14 192 4.43e-24 95.5
Msa1206650 AT5G20020.1 28.796 191 129 3 14 200 14 201 6.55e-24 95.1
Msa1206650 AT5G20010.1 31.056 161 108 2 14 174 14 171 7.67e-24 94.7
Msa1206650 AT3G51300.1 32.934 167 98 5 15 169 8 172 8.95e-24 94.0
Msa1206650 AT1G75840.1 32.544 169 100 5 13 169 6 172 2.15e-23 92.8
Msa1206650 AT2G17800.2 32.544 169 100 5 13 169 6 172 4.21e-23 92.0
Msa1206650 AT2G17800.1 32.544 169 100 5 13 169 6 172 4.21e-23 92.0
Msa1206650 AT4G35950.1 32.544 169 100 5 13 169 6 172 6.70e-23 91.7
Msa1206650 AT5G45970.1 30.539 167 102 5 13 167 6 170 3.85e-22 89.7
Msa1206650 AT5G46025.1 40.187 107 63 1 75 180 6 112 8.11e-22 86.7
Msa1206650 AT1G20090.1 31.361 169 102 5 13 169 5 171 1.07e-21 88.6
Msa1206650 AT3G48040.1 30.178 169 104 5 13 169 8 174 1.02e-20 86.3
Msa1206650 AT5G62880.1 29.885 174 108 5 13 174 8 179 5.54e-20 84.3
Msa1206650 AT3G48040.2 33.333 123 79 3 13 134 8 128 3.88e-19 81.3
Msa1206650 AT5G62880.2 34.146 123 78 3 13 134 8 128 5.18e-19 81.3
Msa1206650 AT4G35020.4 35.965 114 70 3 15 127 8 119 1.71e-18 79.0
Msa1206650 AT4G35020.5 35.965 114 70 3 15 127 8 119 1.71e-18 79.0
Msa1206650 AT5G37680.1 34.058 138 81 4 14 150 20 148 3.33e-14 68.6
Msa1206650 AT5G67560.1 33.962 159 95 5 14 167 20 173 3.81e-14 68.2
Msa1206650 AT3G51290.2 32.394 142 82 5 40 169 634 773 4.33e-14 70.9
Msa1206650 AT3G49870.2 31.609 174 99 6 14 177 1 164 5.24e-14 67.4
Msa1206650 AT3G49870.1 31.111 180 104 6 8 177 14 183 1.08e-13 67.0
Msa1206650 AT3G22950.1 29.688 128 84 3 6 132 10 132 4.02e-12 62.8
Msa1206650 AT3G22950.2 29.688 128 84 3 6 132 10 132 4.02e-12 62.8
Msa1206650 AT3G49860.1 28.205 156 104 4 14 165 12 163 2.50e-11 60.5
Msa1206650 AT5G37680.2 35.780 109 60 4 43 150 2 101 3.70e-11 58.9

Find 60 sgRNAs with CRISPR-Local

Find 286 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACTGGTTGTCCTCGAATT+TGG 0.168291 8_1:-63716188 None:intergenic
ACTTACAATTTGAAGTTTAA+TGG 0.206147 8_1:-63714998 None:intergenic
CCTCGAATTTGGACTGTTGC+AGG 0.237144 8_1:-63716177 None:intergenic
CAGGCATTTGCAGATGAAAT+TGG 0.240708 8_1:+63715897 Msa1206650:intron
CTGCTGGTCAAGAACGTTTC+CGG 0.274066 8_1:+63715471 Msa1206650:CDS
AATAATATCATATCTTTGAT+TGG 0.289625 8_1:+63714237 None:intergenic
TGAGTAACGTAAAATGTGTT+TGG 0.320714 8_1:-63716458 None:intergenic
GAGTAACGTAAAATGTGTTT+GGG 0.326797 8_1:-63716457 None:intergenic
TGAAATTGGAATTCCTTTCA+TGG 0.332549 8_1:+63715911 Msa1206650:CDS
ATAATATCATATCTTTGATT+GGG 0.352683 8_1:+63714238 None:intergenic
TCACTTATCTCTTTAGTTGT+TGG 0.357342 8_1:+63716499 Msa1206650:three_prime_UTR
CAATGTGGAACAAGCTTTCA+TGG 0.386569 8_1:+63715956 Msa1206650:CDS
TAACATTGTTAAAGCTCTCT+TGG 0.389666 8_1:-63715650 None:intergenic
ACGAGCAGCAACCAGCCTTC+TGG 0.395079 8_1:-63716212 None:intergenic
TCTTAGAGACCAAGTATTCA+TGG 0.395534 8_1:+63716234 Msa1206650:exon
TACTCAATAGCTTCCCGTAT+TGG 0.396704 8_1:+63716475 Msa1206650:three_prime_UTR
AATTTGGACTGTTGCAGGCC+TGG 0.409513 8_1:-63716172 None:intergenic
AATGTTAACAAGCTTCTAGT+TGG 0.417837 8_1:+63715711 Msa1206650:CDS
CTTCTAAGACCATGAATACT+TGG 0.425115 8_1:-63716243 None:intergenic
AAGAGATAAGTGAGCCAATA+CGG 0.427083 8_1:-63716489 None:intergenic
TGTTGCAGGCCTGGCACTGT+TGG 0.432139 8_1:-63716163 None:intergenic
CAACCAGCCTTCTGGTTCAC+TGG 0.436527 8_1:-63716204 None:intergenic
GAAAATCCGCACTGTTGAGC+AGG 0.471772 8_1:+63714966 Msa1206650:intron
GTCAACCTGCCAACAGTGCC+AGG 0.473985 8_1:+63716154 Msa1206650:CDS
AGCTACTATCGTGGGGCTCA+TGG 0.480037 8_1:+63715506 Msa1206650:CDS
ATAGTCACTGAAAATAAGAT+AGG 0.486963 8_1:-63714265 None:intergenic
GAAAGCTTGTTCCACATTGG+AGG 0.502915 8_1:-63715952 None:intergenic
TTGATTGGAGATTCTGGTGT+GGG 0.506444 8_1:+63714303 Msa1206650:CDS
ATTGTATAGTGGGACACTGC+TGG 0.512939 8_1:+63715455 Msa1206650:intron
TCCGCACTGTTGAGCAGGAT+GGG 0.520866 8_1:+63714971 Msa1206650:CDS
TTAGCACTAGTTTCCATGAA+AGG 0.524383 8_1:-63715924 None:intergenic
ATCCGCACTGTTGAGCAGGA+TGG 0.528930 8_1:+63714970 Msa1206650:CDS
AATCACCTTTGCTGTTTCAG+AGG 0.533991 8_1:-63715769 None:intergenic
AGTAGCTGCTAGTTATAGTC+CGG 0.542099 8_1:-63715490 None:intergenic
TAATATCATATCTTTGATTG+GGG 0.543013 8_1:+63714239 None:intergenic
TAACTAGCAGCTACTATCGT+GGG 0.548282 8_1:+63715498 Msa1206650:CDS
AGAGATAAGTGAGCCAATAC+GGG 0.555982 8_1:-63716488 None:intergenic
TGTGTTTGGGGCAACGAACA+AGG 0.563689 8_1:-63716444 None:intergenic
GACAGCTATATCAGTACCAT+CGG 0.567693 8_1:+63714843 Msa1206650:CDS
CAGAACTTACAGCAAACCTC+AGG 0.568006 8_1:-63714341 None:intergenic
TGGAAACAAGAGCGATCTCT+CGG 0.568213 8_1:+63715731 Msa1206650:CDS
GCAAGTCATGTCTCCTCCTG+AGG 0.576917 8_1:+63714325 Msa1206650:CDS
TGGTACTGATATAGCTGTCA+AGG 0.577148 8_1:-63714839 None:intergenic
CCTGCAACAGTCCAAATTCG+AGG 0.584589 8_1:+63716177 Msa1206650:CDS
CATGAAAGCTTGTTCCACAT+TGG 0.594293 8_1:-63715955 None:intergenic
CAACCAGTGAACCAGAAGGC+TGG 0.595916 8_1:+63716201 Msa1206650:CDS
GGAACAAGCTTTCATGGCCA+TGG 0.601950 8_1:+63715962 Msa1206650:CDS
GTTGATTGGAGATTCTGGTG+TGG 0.609925 8_1:+63714302 Msa1206650:CDS
AACTTACAGCAAACCTCAGG+AGG 0.618028 8_1:-63714338 None:intergenic
AGAAGTTCCATGTGTCTCTG+TGG 0.620705 8_1:+63716261 Msa1206650:three_prime_UTR
ATAACTAGCAGCTACTATCG+TGG 0.626182 8_1:+63715497 Msa1206650:CDS
GCTTACAAAGTCCACTCCGA+TGG 0.633467 8_1:-63714859 None:intergenic
GCTTTAACAATGTTAAGCAG+TGG 0.636327 8_1:+63715658 Msa1206650:CDS
TTACATGCCACAGAGACACA+TGG 0.640787 8_1:-63716268 None:intergenic
TGTGTCCTCTGAAACAGCAA+AGG 0.645238 8_1:+63715764 Msa1206650:CDS
TCCCATCCTGCTCAACAGTG+CGG 0.647894 8_1:-63714972 None:intergenic
AGTAACGTAAAATGTGTTTG+GGG 0.658888 8_1:-63716456 None:intergenic
AACTAGCAGCTACTATCGTG+GGG 0.674225 8_1:+63715499 Msa1206650:CDS
CTATATCAGTACCATCGGAG+TGG 0.681244 8_1:+63714848 Msa1206650:CDS
AGGACAACCAGTGAACCAGA+AGG 0.685270 8_1:+63716197 Msa1206650:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATGTAATAGTAAAAAAT+GGG - chr8_1:63714446-63714465 None:intergenic 10.0%
!! AAAATGTAATAGTAAAAAAT+GGG - chr8_1:63714446-63714465 None:intergenic 10.0%
!! GAAAAAAAAAAAAAAAACAG+AGG - chr8_1:63714369-63714388 None:intergenic 15.0%
!! GAAAATGTAATAGTAAAAAA+TGG - chr8_1:63714447-63714466 None:intergenic 15.0%
!!! CTTTTTAAATTTTTTTTTGC+AGG + chr8_1:63715608-63715627 Msa1206650:intron 15.0%
!! ACATCAAAATATTAGATTAA+AGG - chr8_1:63715860-63715879 None:intergenic 15.0%
!! AAAAAATTATGTACAAAAGT+AGG + chr8_1:63716397-63716416 Msa1206650:three_prime_UTR 15.0%
!! GAAAAAAAAAAAAAAAACAG+AGG - chr8_1:63714369-63714388 None:intergenic 15.0%
!! GAAAATGTAATAGTAAAAAA+TGG - chr8_1:63714447-63714466 None:intergenic 15.0%
!!! CTTTTTAAATTTTTTTTTGC+AGG + chr8_1:63715608-63715627 Msa1206650:intron 15.0%
!! ACATCAAAATATTAGATTAA+AGG - chr8_1:63715860-63715879 None:intergenic 15.0%
!! AAAAAATTATGTACAAAAGT+AGG + chr8_1:63716397-63716416 Msa1206650:three_prime_UTR 15.0%
!!! TTTTTTTCCTTCTTTAGTTA+TGG + chr8_1:63714381-63714400 Msa1206650:intron 20.0%
!! AGTTGAATATAAAGAACATA+TGG + chr8_1:63714473-63714492 Msa1206650:intron 20.0%
!! ACTTCATAAAATATCATTCA+AGG - chr8_1:63714766-63714785 None:intergenic 20.0%
!!! TTGAATGATATTTTATGAAG+TGG + chr8_1:63714765-63714784 Msa1206650:intron 20.0%
!! ATAAGTTTAAATGGTAGATA+TGG - chr8_1:63714888-63714907 None:intergenic 20.0%
!! AACATGGAAATAAGTTTAAA+TGG - chr8_1:63714897-63714916 None:intergenic 20.0%
!!! ACTTACAATTTGAAGTTTAA+TGG - chr8_1:63715001-63715020 None:intergenic 20.0%
!! TTTAGATGTAGAAAACAAAT+TGG - chr8_1:63715111-63715130 None:intergenic 20.0%
!! TTATATGTCCATTTGATAAA+TGG + chr8_1:63715246-63715265 Msa1206650:intron 20.0%
!! TTGATTTCCTAATATTCAAT+GGG - chr8_1:63715347-63715366 None:intergenic 20.0%
!!! ATTGATTTCCTAATATTCAA+TGG - chr8_1:63715348-63715367 None:intergenic 20.0%
!!! TGCATTTTTTTATTGTATAG+TGG + chr8_1:63715444-63715463 Msa1206650:intron 20.0%
!!! GCATTTTTTTATTGTATAGT+GGG + chr8_1:63715445-63715464 Msa1206650:intron 20.0%
!! CAAAATATTAGATTAAAGGA+AGG - chr8_1:63715856-63715875 None:intergenic 20.0%
!!! TTTTTTTCCTTCTTTAGTTA+TGG + chr8_1:63714381-63714400 Msa1206650:intron 20.0%
!! AGTTGAATATAAAGAACATA+TGG + chr8_1:63714473-63714492 Msa1206650:intron 20.0%
!! ACTTCATAAAATATCATTCA+AGG - chr8_1:63714766-63714785 None:intergenic 20.0%
!!! TTGAATGATATTTTATGAAG+TGG + chr8_1:63714765-63714784 Msa1206650:intron 20.0%
!! ATAAGTTTAAATGGTAGATA+TGG - chr8_1:63714888-63714907 None:intergenic 20.0%
!! AACATGGAAATAAGTTTAAA+TGG - chr8_1:63714897-63714916 None:intergenic 20.0%
!!! ACTTACAATTTGAAGTTTAA+TGG - chr8_1:63715001-63715020 None:intergenic 20.0%
!! TTTAGATGTAGAAAACAAAT+TGG - chr8_1:63715111-63715130 None:intergenic 20.0%
!! TTATATGTCCATTTGATAAA+TGG + chr8_1:63715246-63715265 Msa1206650:intron 20.0%
!! TTGATTTCCTAATATTCAAT+GGG - chr8_1:63715347-63715366 None:intergenic 20.0%
!!! ATTGATTTCCTAATATTCAA+TGG - chr8_1:63715348-63715367 None:intergenic 20.0%
!!! TGCATTTTTTTATTGTATAG+TGG + chr8_1:63715444-63715463 Msa1206650:intron 20.0%
!!! GCATTTTTTTATTGTATAGT+GGG + chr8_1:63715445-63715464 Msa1206650:intron 20.0%
!! CAAAATATTAGATTAAAGGA+AGG - chr8_1:63715856-63715875 None:intergenic 20.0%
! ATAGTCACTGAAAATAAGAT+AGG - chr8_1:63714268-63714287 None:intergenic 25.0%
!! AATGATATTTTATGAAGTGG+AGG + chr8_1:63714768-63714787 Msa1206650:intron 25.0%
!! TTTTTCTTTTTCTGTGAAAC+AGG + chr8_1:63714806-63714825 Msa1206650:intron 25.0%
! TACTAAAGTTGAAAACAACA+TGG - chr8_1:63714913-63714932 None:intergenic 25.0%
! AATACTGAACAAGTTGTTAT+TGG + chr8_1:63715045-63715064 Msa1206650:intron 25.0%
! CATTTGAACAGTATTATTTG+AGG + chr8_1:63715310-63715329 Msa1206650:intron 25.0%
! AAAGTTACCCATTGAATATT+AGG + chr8_1:63715337-63715356 Msa1206650:intron 25.0%
!! AATTTCCTTTTCAAACACTT+CGG + chr8_1:63715365-63715384 Msa1206650:intron 25.0%
!! AATTGTGAGTCTTTTTAAAC+CGG + chr8_1:63715532-63715551 Msa1206650:intron 25.0%
!! ATTTCCTTTTTACATTGTCT+TGG + chr8_1:63715568-63715587 Msa1206650:intron 25.0%
!! TTTCCTTTTTACATTGTCTT+GGG + chr8_1:63715569-63715588 Msa1206650:intron 25.0%
! TATGGAATTTCTGTATGTAA+AGG + chr8_1:63716005-63716024 Msa1206650:intron 25.0%
! GTAAATGAGGTTAATAATCA+AGG - chr8_1:63716102-63716121 None:intergenic 25.0%
! ATAGTCACTGAAAATAAGAT+AGG - chr8_1:63714268-63714287 None:intergenic 25.0%
!! AATGATATTTTATGAAGTGG+AGG + chr8_1:63714768-63714787 Msa1206650:intron 25.0%
!! TTTTTCTTTTTCTGTGAAAC+AGG + chr8_1:63714806-63714825 Msa1206650:intron 25.0%
! TACTAAAGTTGAAAACAACA+TGG - chr8_1:63714913-63714932 None:intergenic 25.0%
! AATACTGAACAAGTTGTTAT+TGG + chr8_1:63715045-63715064 Msa1206650:intron 25.0%
! CATTTGAACAGTATTATTTG+AGG + chr8_1:63715310-63715329 Msa1206650:intron 25.0%
! AAAGTTACCCATTGAATATT+AGG + chr8_1:63715337-63715356 Msa1206650:intron 25.0%
!! AATTTCCTTTTCAAACACTT+CGG + chr8_1:63715365-63715384 Msa1206650:intron 25.0%
!! AATTGTGAGTCTTTTTAAAC+CGG + chr8_1:63715532-63715551 Msa1206650:intron 25.0%
!! ATTTCCTTTTTACATTGTCT+TGG + chr8_1:63715568-63715587 Msa1206650:intron 25.0%
!! TTTCCTTTTTACATTGTCTT+GGG + chr8_1:63715569-63715588 Msa1206650:intron 25.0%
! TATGGAATTTCTGTATGTAA+AGG + chr8_1:63716005-63716024 Msa1206650:intron 25.0%
! GTAAATGAGGTTAATAATCA+AGG - chr8_1:63716102-63716121 None:intergenic 25.0%
! TTGTTCAAGCTTTTGTTGAT+TGG + chr8_1:63714288-63714307 Msa1206650:CDS 30.0%
AAGATGACCATAACTAAAGA+AGG - chr8_1:63714391-63714410 None:intergenic 30.0%
! GAACATATGGATGTGTTTTT+AGG + chr8_1:63714486-63714505 Msa1206650:intron 30.0%
TCTTGACCATAAACAATGTA+AGG + chr8_1:63714587-63714606 Msa1206650:intron 30.0%
!!! GTCATTTTTATGTGAGAAGA+AGG - chr8_1:63714697-63714716 None:intergenic 30.0%
!! ATTTTCTAAAGAAGGAAGCA+TGG - chr8_1:63714952-63714971 None:intergenic 30.0%
!!! TCCATGAGAAGTTTTATTTG+AGG + chr8_1:63715197-63715216 Msa1206650:intron 30.0%
TCCTCAAATAAAACTTCTCA+TGG - chr8_1:63715201-63715220 None:intergenic 30.0%
! AATTGGTAACTGGAAAAACT+TGG - chr8_1:63715278-63715297 None:intergenic 30.0%
AAATGATCCAAGAACACAAT+TGG - chr8_1:63715295-63715314 None:intergenic 30.0%
AAAAGGAAATTGCTGACAAA+GGG - chr8_1:63715558-63715577 None:intergenic 30.0%
AAAAAGGAAATTGCTGACAA+AGG - chr8_1:63715559-63715578 None:intergenic 30.0%
TTTCCCAAGACAATGTAAAA+AGG - chr8_1:63715575-63715594 None:intergenic 30.0%
TAACATTGTTAAAGCTCTCT+TGG - chr8_1:63715653-63715672 None:intergenic 30.0%
! TAACATTTTCACTTGCATAG+CGG - chr8_1:63715698-63715717 None:intergenic 30.0%
AATGTTAACAAGCTTCTAGT+TGG + chr8_1:63715711-63715730 Msa1206650:CDS 30.0%
AAAAAAATGTCACTGGAAGT+GGG - chr8_1:63715828-63715847 None:intergenic 30.0%
AAAAAAAATGTCACTGGAAG+TGG - chr8_1:63715829-63715848 None:intergenic 30.0%
GGACTTAAAAAAAATGTCAC+TGG - chr8_1:63715835-63715854 None:intergenic 30.0%
TGAAATTGGAATTCCTTTCA+TGG + chr8_1:63715911-63715930 Msa1206650:CDS 30.0%
GCTGAAATTAAAAACAGGTA+TGG + chr8_1:63715987-63716006 Msa1206650:intron 30.0%
! CAGTATCTATTGACTAAACA+AGG - chr8_1:63716074-63716093 None:intergenic 30.0%
AGTAACGTAAAATGTGTTTG+GGG - chr8_1:63716459-63716478 None:intergenic 30.0%
GAGTAACGTAAAATGTGTTT+GGG - chr8_1:63716460-63716479 None:intergenic 30.0%
TGAGTAACGTAAAATGTGTT+TGG - chr8_1:63716461-63716480 None:intergenic 30.0%
TCACTTATCTCTTTAGTTGT+TGG + chr8_1:63716499-63716518 Msa1206650:three_prime_UTR 30.0%
! TTGTTCAAGCTTTTGTTGAT+TGG + chr8_1:63714288-63714307 Msa1206650:CDS 30.0%
AAGATGACCATAACTAAAGA+AGG - chr8_1:63714391-63714410 None:intergenic 30.0%
! GAACATATGGATGTGTTTTT+AGG + chr8_1:63714486-63714505 Msa1206650:intron 30.0%
TCTTGACCATAAACAATGTA+AGG + chr8_1:63714587-63714606 Msa1206650:intron 30.0%
!!! GTCATTTTTATGTGAGAAGA+AGG - chr8_1:63714697-63714716 None:intergenic 30.0%
!! ATTTTCTAAAGAAGGAAGCA+TGG - chr8_1:63714952-63714971 None:intergenic 30.0%
!!! TCCATGAGAAGTTTTATTTG+AGG + chr8_1:63715197-63715216 Msa1206650:intron 30.0%
TCCTCAAATAAAACTTCTCA+TGG - chr8_1:63715201-63715220 None:intergenic 30.0%
! AATTGGTAACTGGAAAAACT+TGG - chr8_1:63715278-63715297 None:intergenic 30.0%
AAATGATCCAAGAACACAAT+TGG - chr8_1:63715295-63715314 None:intergenic 30.0%
AAAAGGAAATTGCTGACAAA+GGG - chr8_1:63715558-63715577 None:intergenic 30.0%
AAAAAGGAAATTGCTGACAA+AGG - chr8_1:63715559-63715578 None:intergenic 30.0%
TTTCCCAAGACAATGTAAAA+AGG - chr8_1:63715575-63715594 None:intergenic 30.0%
TAACATTGTTAAAGCTCTCT+TGG - chr8_1:63715653-63715672 None:intergenic 30.0%
! TAACATTTTCACTTGCATAG+CGG - chr8_1:63715698-63715717 None:intergenic 30.0%
AATGTTAACAAGCTTCTAGT+TGG + chr8_1:63715711-63715730 Msa1206650:CDS 30.0%
AAAAAAATGTCACTGGAAGT+GGG - chr8_1:63715828-63715847 None:intergenic 30.0%
AAAAAAAATGTCACTGGAAG+TGG - chr8_1:63715829-63715848 None:intergenic 30.0%
GGACTTAAAAAAAATGTCAC+TGG - chr8_1:63715835-63715854 None:intergenic 30.0%
TGAAATTGGAATTCCTTTCA+TGG + chr8_1:63715911-63715930 Msa1206650:CDS 30.0%
GCTGAAATTAAAAACAGGTA+TGG + chr8_1:63715987-63716006 Msa1206650:intron 30.0%
! CAGTATCTATTGACTAAACA+AGG - chr8_1:63716074-63716093 None:intergenic 30.0%
AGTAACGTAAAATGTGTTTG+GGG - chr8_1:63716459-63716478 None:intergenic 30.0%
GAGTAACGTAAAATGTGTTT+GGG - chr8_1:63716460-63716479 None:intergenic 30.0%
TGAGTAACGTAAAATGTGTT+TGG - chr8_1:63716461-63716480 None:intergenic 30.0%
TCACTTATCTCTTTAGTTGT+TGG + chr8_1:63716499-63716518 Msa1206650:three_prime_UTR 30.0%
!! CTTTTGTTGATTGGAGATTC+TGG + chr8_1:63714297-63714316 Msa1206650:CDS 35.0%
!! AGGCATTGAAAAAGTGTGTA+TGG + chr8_1:63714506-63714525 Msa1206650:intron 35.0%
TATATGCACCAGAGAGTAAA+AGG + chr8_1:63714558-63714577 Msa1206650:intron 35.0%
! GATTTACTCCTTTTACTCTC+TGG - chr8_1:63714569-63714588 None:intergenic 35.0%
CCTTGACCTTACATTGTTTA+TGG - chr8_1:63714596-63714615 None:intergenic 35.0%
CCATAAACAATGTAAGGTCA+AGG + chr8_1:63714593-63714612 Msa1206650:intron 35.0%
CTATTTGTGGTTTGATCGAA+CGG + chr8_1:63714660-63714679 Msa1206650:intron 35.0%
!! TTATTGGTTCTGATCTCTGA+TGG + chr8_1:63715061-63715080 Msa1206650:intron 35.0%
!! TATTGGTTCTGATCTCTGAT+GGG + chr8_1:63715062-63715081 Msa1206650:intron 35.0%
GGAATGAGCCATTTATCAAA+TGG - chr8_1:63715257-63715276 None:intergenic 35.0%
ACATTGTCTTGGGAAAACAA+TGG + chr8_1:63715579-63715598 Msa1206650:intron 35.0%
GCTTTAACAATGTTAAGCAG+TGG + chr8_1:63715658-63715677 Msa1206650:CDS 35.0%
! TTAGCACTAGTTTCCATGAA+AGG - chr8_1:63715927-63715946 None:intergenic 35.0%
!!! GTTTTTAATTTCAGCAGCCA+TGG - chr8_1:63715982-63716001 None:intergenic 35.0%
TGGCTGCTGAAATTAAAAAC+AGG + chr8_1:63715982-63716001 Msa1206650:CDS 35.0%
AGTTCACAAACTCATGCAAA+AGG - chr8_1:63716043-63716062 None:intergenic 35.0%
! CCATTCTGTGAAAAAAGTAC+CGG - chr8_1:63716131-63716150 None:intergenic 35.0%
TCTTAGAGACCAAGTATTCA+TGG + chr8_1:63716234-63716253 Msa1206650:exon 35.0%
!! CTTCTAAGACCATGAATACT+TGG - chr8_1:63716246-63716265 None:intergenic 35.0%
AAGAGATAAGTGAGCCAATA+CGG - chr8_1:63716492-63716511 None:intergenic 35.0%
!! CTTTTGTTGATTGGAGATTC+TGG + chr8_1:63714297-63714316 Msa1206650:CDS 35.0%
!! AGGCATTGAAAAAGTGTGTA+TGG + chr8_1:63714506-63714525 Msa1206650:intron 35.0%
TATATGCACCAGAGAGTAAA+AGG + chr8_1:63714558-63714577 Msa1206650:intron 35.0%
! GATTTACTCCTTTTACTCTC+TGG - chr8_1:63714569-63714588 None:intergenic 35.0%
CCTTGACCTTACATTGTTTA+TGG - chr8_1:63714596-63714615 None:intergenic 35.0%
CCATAAACAATGTAAGGTCA+AGG + chr8_1:63714593-63714612 Msa1206650:intron 35.0%
CTATTTGTGGTTTGATCGAA+CGG + chr8_1:63714660-63714679 Msa1206650:intron 35.0%
!! TTATTGGTTCTGATCTCTGA+TGG + chr8_1:63715061-63715080 Msa1206650:intron 35.0%
!! TATTGGTTCTGATCTCTGAT+GGG + chr8_1:63715062-63715081 Msa1206650:intron 35.0%
GGAATGAGCCATTTATCAAA+TGG - chr8_1:63715257-63715276 None:intergenic 35.0%
ACATTGTCTTGGGAAAACAA+TGG + chr8_1:63715579-63715598 Msa1206650:intron 35.0%
GCTTTAACAATGTTAAGCAG+TGG + chr8_1:63715658-63715677 Msa1206650:CDS 35.0%
! TTAGCACTAGTTTCCATGAA+AGG - chr8_1:63715927-63715946 None:intergenic 35.0%
!!! GTTTTTAATTTCAGCAGCCA+TGG - chr8_1:63715982-63716001 None:intergenic 35.0%
TGGCTGCTGAAATTAAAAAC+AGG + chr8_1:63715982-63716001 Msa1206650:CDS 35.0%
AGTTCACAAACTCATGCAAA+AGG - chr8_1:63716043-63716062 None:intergenic 35.0%
! CCATTCTGTGAAAAAAGTAC+CGG - chr8_1:63716131-63716150 None:intergenic 35.0%
TCTTAGAGACCAAGTATTCA+TGG + chr8_1:63716234-63716253 Msa1206650:exon 35.0%
!! CTTCTAAGACCATGAATACT+TGG - chr8_1:63716246-63716265 None:intergenic 35.0%
AAGAGATAAGTGAGCCAATA+CGG - chr8_1:63716492-63716511 None:intergenic 35.0%
TTGATTGGAGATTCTGGTGT+GGG + chr8_1:63714303-63714322 Msa1206650:CDS 40.0%
TACATAGTGAGATTGTGAGC+AGG + chr8_1:63714534-63714553 Msa1206650:intron 40.0%
CAGAGTCACATCTCTATTTG+TGG + chr8_1:63714647-63714666 Msa1206650:intron 40.0%
!! TGGTACTGATATAGCTGTCA+AGG - chr8_1:63714842-63714861 None:intergenic 40.0%
GACAGCTATATCAGTACCAT+CGG + chr8_1:63714843-63714862 Msa1206650:CDS 40.0%
!! CAGTGCGGATTTTCTAAAGA+AGG - chr8_1:63714960-63714979 None:intergenic 40.0%
! CCAAGAACACAATTGGTAAC+TGG - chr8_1:63715288-63715307 None:intergenic 40.0%
CCAGTTACCAATTGTGTTCT+TGG + chr8_1:63715285-63715304 Msa1206650:intron 40.0%
! TTGAGCCGAAGTGTTTGAAA+AGG - chr8_1:63715373-63715392 None:intergenic 40.0%
AACACTTCGGCTCAATTATC+TGG + chr8_1:63715378-63715397 Msa1206650:intron 40.0%
ACACTTCGGCTCAATTATCT+GGG + chr8_1:63715379-63715398 Msa1206650:intron 40.0%
AGTAGCTGCTAGTTATAGTC+CGG - chr8_1:63715493-63715512 None:intergenic 40.0%
ATAACTAGCAGCTACTATCG+TGG + chr8_1:63715497-63715516 Msa1206650:CDS 40.0%
TAACTAGCAGCTACTATCGT+GGG + chr8_1:63715498-63715517 Msa1206650:CDS 40.0%
!! AATCACCTTTGCTGTTTCAG+AGG - chr8_1:63715772-63715791 None:intergenic 40.0%
TGGAAGTGGGACATGTATAT+GGG - chr8_1:63715815-63715834 None:intergenic 40.0%
! GTTTGTGCCTTTTGTACATC+AGG + chr8_1:63715878-63715897 Msa1206650:intron 40.0%
GCAAATGCCTGATGTACAAA+AGG - chr8_1:63715888-63715907 None:intergenic 40.0%
CAGGCATTTGCAGATGAAAT+TGG + chr8_1:63715897-63715916 Msa1206650:intron 40.0%
TGCTAAAAATGCCTCCAATG+TGG + chr8_1:63715941-63715960 Msa1206650:CDS 40.0%
! CATGAAAGCTTGTTCCACAT+TGG - chr8_1:63715958-63715977 None:intergenic 40.0%
CAATGTGGAACAAGCTTTCA+TGG + chr8_1:63715956-63715975 Msa1206650:CDS 40.0%
!!! CCGGTACTTTTTTCACAGAA+TGG + chr8_1:63716128-63716147 Msa1206650:intron 40.0%
TACTCAATAGCTTCCCGTAT+TGG + chr8_1:63716475-63716494 Msa1206650:three_prime_UTR 40.0%
AGAGATAAGTGAGCCAATAC+GGG - chr8_1:63716491-63716510 None:intergenic 40.0%
TTGATTGGAGATTCTGGTGT+GGG + chr8_1:63714303-63714322 Msa1206650:CDS 40.0%
TACATAGTGAGATTGTGAGC+AGG + chr8_1:63714534-63714553 Msa1206650:intron 40.0%
CAGAGTCACATCTCTATTTG+TGG + chr8_1:63714647-63714666 Msa1206650:intron 40.0%
!! TGGTACTGATATAGCTGTCA+AGG - chr8_1:63714842-63714861 None:intergenic 40.0%
GACAGCTATATCAGTACCAT+CGG + chr8_1:63714843-63714862 Msa1206650:CDS 40.0%
!! CAGTGCGGATTTTCTAAAGA+AGG - chr8_1:63714960-63714979 None:intergenic 40.0%
! CCAAGAACACAATTGGTAAC+TGG - chr8_1:63715288-63715307 None:intergenic 40.0%
CCAGTTACCAATTGTGTTCT+TGG + chr8_1:63715285-63715304 Msa1206650:intron 40.0%
! TTGAGCCGAAGTGTTTGAAA+AGG - chr8_1:63715373-63715392 None:intergenic 40.0%
AACACTTCGGCTCAATTATC+TGG + chr8_1:63715378-63715397 Msa1206650:intron 40.0%
ACACTTCGGCTCAATTATCT+GGG + chr8_1:63715379-63715398 Msa1206650:intron 40.0%
AGTAGCTGCTAGTTATAGTC+CGG - chr8_1:63715493-63715512 None:intergenic 40.0%
ATAACTAGCAGCTACTATCG+TGG + chr8_1:63715497-63715516 Msa1206650:CDS 40.0%
TAACTAGCAGCTACTATCGT+GGG + chr8_1:63715498-63715517 Msa1206650:CDS 40.0%
!! AATCACCTTTGCTGTTTCAG+AGG - chr8_1:63715772-63715791 None:intergenic 40.0%
TGGAAGTGGGACATGTATAT+GGG - chr8_1:63715815-63715834 None:intergenic 40.0%
! GTTTGTGCCTTTTGTACATC+AGG + chr8_1:63715878-63715897 Msa1206650:intron 40.0%
GCAAATGCCTGATGTACAAA+AGG - chr8_1:63715888-63715907 None:intergenic 40.0%
CAGGCATTTGCAGATGAAAT+TGG + chr8_1:63715897-63715916 Msa1206650:intron 40.0%
TGCTAAAAATGCCTCCAATG+TGG + chr8_1:63715941-63715960 Msa1206650:CDS 40.0%
! CATGAAAGCTTGTTCCACAT+TGG - chr8_1:63715958-63715977 None:intergenic 40.0%
CAATGTGGAACAAGCTTTCA+TGG + chr8_1:63715956-63715975 Msa1206650:CDS 40.0%
!!! CCGGTACTTTTTTCACAGAA+TGG + chr8_1:63716128-63716147 Msa1206650:intron 40.0%
TACTCAATAGCTTCCCGTAT+TGG + chr8_1:63716475-63716494 Msa1206650:three_prime_UTR 40.0%
AGAGATAAGTGAGCCAATAC+GGG - chr8_1:63716491-63716510 None:intergenic 40.0%
GTTGATTGGAGATTCTGGTG+TGG + chr8_1:63714302-63714321 Msa1206650:CDS 45.0%
AACTTACAGCAAACCTCAGG+AGG - chr8_1:63714341-63714360 None:intergenic 45.0%
CAGAACTTACAGCAAACCTC+AGG - chr8_1:63714344-63714363 None:intergenic 45.0%
ACTCTCTCACTTGACAGTCA+AGG + chr8_1:63714715-63714734 Msa1206650:intron 45.0%
CTATATCAGTACCATCGGAG+TGG + chr8_1:63714848-63714867 Msa1206650:CDS 45.0%
! TCGGCTCAATTATCTGGGAT+TGG + chr8_1:63715384-63715403 Msa1206650:intron 45.0%
! ATTGTATAGTGGGACACTGC+TGG + chr8_1:63715455-63715474 Msa1206650:intron 45.0%
AACTAGCAGCTACTATCGTG+GGG + chr8_1:63715499-63715518 Msa1206650:CDS 45.0%
TGGAAACAAGAGCGATCTCT+CGG + chr8_1:63715731-63715750 Msa1206650:CDS 45.0%
!! TGTGTCCTCTGAAACAGCAA+AGG + chr8_1:63715764-63715783 Msa1206650:CDS 45.0%
GGAAGTGGGACATGTATATG+GGG - chr8_1:63715814-63715833 None:intergenic 45.0%
CTGGAAGTGGGACATGTATA+TGG - chr8_1:63715816-63715835 None:intergenic 45.0%
! GAAAGCTTGTTCCACATTGG+AGG - chr8_1:63715955-63715974 None:intergenic 45.0%
TAACCTCATTTACTCGTCGC+CGG + chr8_1:63716109-63716128 Msa1206650:intron 45.0%
! TCACTGGTTGTCCTCGAATT+TGG - chr8_1:63716191-63716210 None:intergenic 45.0%
AGAAGTTCCATGTGTCTCTG+TGG + chr8_1:63716261-63716280 Msa1206650:three_prime_UTR 45.0%
TTACATGCCACAGAGACACA+TGG - chr8_1:63716271-63716290 None:intergenic 45.0%
GTTGATTGGAGATTCTGGTG+TGG + chr8_1:63714302-63714321 Msa1206650:CDS 45.0%
AACTTACAGCAAACCTCAGG+AGG - chr8_1:63714341-63714360 None:intergenic 45.0%
CAGAACTTACAGCAAACCTC+AGG - chr8_1:63714344-63714363 None:intergenic 45.0%
ACTCTCTCACTTGACAGTCA+AGG + chr8_1:63714715-63714734 Msa1206650:intron 45.0%
CTATATCAGTACCATCGGAG+TGG + chr8_1:63714848-63714867 Msa1206650:CDS 45.0%
! TCGGCTCAATTATCTGGGAT+TGG + chr8_1:63715384-63715403 Msa1206650:intron 45.0%
! ATTGTATAGTGGGACACTGC+TGG + chr8_1:63715455-63715474 Msa1206650:intron 45.0%
AACTAGCAGCTACTATCGTG+GGG + chr8_1:63715499-63715518 Msa1206650:CDS 45.0%
TGGAAACAAGAGCGATCTCT+CGG + chr8_1:63715731-63715750 Msa1206650:CDS 45.0%
!! TGTGTCCTCTGAAACAGCAA+AGG + chr8_1:63715764-63715783 Msa1206650:CDS 45.0%
GGAAGTGGGACATGTATATG+GGG - chr8_1:63715814-63715833 None:intergenic 45.0%
CTGGAAGTGGGACATGTATA+TGG - chr8_1:63715816-63715835 None:intergenic 45.0%
! GAAAGCTTGTTCCACATTGG+AGG - chr8_1:63715955-63715974 None:intergenic 45.0%
TAACCTCATTTACTCGTCGC+CGG + chr8_1:63716109-63716128 Msa1206650:intron 45.0%
! TCACTGGTTGTCCTCGAATT+TGG - chr8_1:63716191-63716210 None:intergenic 45.0%
AGAAGTTCCATGTGTCTCTG+TGG + chr8_1:63716261-63716280 Msa1206650:three_prime_UTR 45.0%
TTACATGCCACAGAGACACA+TGG - chr8_1:63716271-63716290 None:intergenic 45.0%
GCTTACAAAGTCCACTCCGA+TGG - chr8_1:63714862-63714881 None:intergenic 50.0%
GAAAATCCGCACTGTTGAGC+AGG + chr8_1:63714966-63714985 Msa1206650:intron 50.0%
CTGCTGGTCAAGAACGTTTC+CGG + chr8_1:63715471-63715490 Msa1206650:CDS 50.0%
GGAAATTGCTGACAAAGGGC+CGG - chr8_1:63715554-63715573 None:intergenic 50.0%
GGAACAAGCTTTCATGGCCA+TGG + chr8_1:63715962-63715981 Msa1206650:CDS 50.0%
AATTTGGACTGTTGCAGGCC+TGG - chr8_1:63716175-63716194 None:intergenic 50.0%
CCTCGAATTTGGACTGTTGC+AGG - chr8_1:63716180-63716199 None:intergenic 50.0%
CCTGCAACAGTCCAAATTCG+AGG + chr8_1:63716177-63716196 Msa1206650:CDS 50.0%
AGGACAACCAGTGAACCAGA+AGG + chr8_1:63716197-63716216 Msa1206650:CDS 50.0%
! TGTGTTTGGGGCAACGAACA+AGG - chr8_1:63716447-63716466 None:intergenic 50.0%
GCTTACAAAGTCCACTCCGA+TGG - chr8_1:63714862-63714881 None:intergenic 50.0%
GAAAATCCGCACTGTTGAGC+AGG + chr8_1:63714966-63714985 Msa1206650:intron 50.0%
CTGCTGGTCAAGAACGTTTC+CGG + chr8_1:63715471-63715490 Msa1206650:CDS 50.0%
GGAAATTGCTGACAAAGGGC+CGG - chr8_1:63715554-63715573 None:intergenic 50.0%
GGAACAAGCTTTCATGGCCA+TGG + chr8_1:63715962-63715981 Msa1206650:CDS 50.0%
AATTTGGACTGTTGCAGGCC+TGG - chr8_1:63716175-63716194 None:intergenic 50.0%
CCTCGAATTTGGACTGTTGC+AGG - chr8_1:63716180-63716199 None:intergenic 50.0%
CCTGCAACAGTCCAAATTCG+AGG + chr8_1:63716177-63716196 Msa1206650:CDS 50.0%
AGGACAACCAGTGAACCAGA+AGG + chr8_1:63716197-63716216 Msa1206650:CDS 50.0%
! TGTGTTTGGGGCAACGAACA+AGG - chr8_1:63716447-63716466 None:intergenic 50.0%
GCAAGTCATGTCTCCTCCTG+AGG + chr8_1:63714325-63714344 Msa1206650:CDS 55.0%
ATCCGCACTGTTGAGCAGGA+TGG + chr8_1:63714970-63714989 Msa1206650:CDS 55.0%
TCCGCACTGTTGAGCAGGAT+GGG + chr8_1:63714971-63714990 Msa1206650:CDS 55.0%
TCCCATCCTGCTCAACAGTG+CGG - chr8_1:63714975-63714994 None:intergenic 55.0%
AGCTACTATCGTGGGGCTCA+TGG + chr8_1:63715506-63715525 Msa1206650:CDS 55.0%
! GTACCGGCGACGAGTAAATG+AGG - chr8_1:63716115-63716134 None:intergenic 55.0%
CAACCAGTGAACCAGAAGGC+TGG + chr8_1:63716201-63716220 Msa1206650:CDS 55.0%
!! CAACCAGCCTTCTGGTTCAC+TGG - chr8_1:63716207-63716226 None:intergenic 55.0%
GCAAGTCATGTCTCCTCCTG+AGG + chr8_1:63714325-63714344 Msa1206650:CDS 55.0%
ATCCGCACTGTTGAGCAGGA+TGG + chr8_1:63714970-63714989 Msa1206650:CDS 55.0%
TCCGCACTGTTGAGCAGGAT+GGG + chr8_1:63714971-63714990 Msa1206650:CDS 55.0%
TCCCATCCTGCTCAACAGTG+CGG - chr8_1:63714975-63714994 None:intergenic 55.0%
AGCTACTATCGTGGGGCTCA+TGG + chr8_1:63715506-63715525 Msa1206650:CDS 55.0%
! GTACCGGCGACGAGTAAATG+AGG - chr8_1:63716115-63716134 None:intergenic 55.0%
CAACCAGTGAACCAGAAGGC+TGG + chr8_1:63716201-63716220 Msa1206650:CDS 55.0%
!! CAACCAGCCTTCTGGTTCAC+TGG - chr8_1:63716207-63716226 None:intergenic 55.0%
GTCAACCTGCCAACAGTGCC+AGG + chr8_1:63716154-63716173 Msa1206650:CDS 60.0%
TGTTGCAGGCCTGGCACTGT+TGG - chr8_1:63716166-63716185 None:intergenic 60.0%
ACGAGCAGCAACCAGCCTTC+TGG - chr8_1:63716215-63716234 None:intergenic 60.0%
GTCAACCTGCCAACAGTGCC+AGG + chr8_1:63716154-63716173 Msa1206650:CDS 60.0%
TGTTGCAGGCCTGGCACTGT+TGG - chr8_1:63716166-63716185 None:intergenic 60.0%
ACGAGCAGCAACCAGCCTTC+TGG - chr8_1:63716215-63716234 None:intergenic 60.0%
GCAGGCCTGGCACTGTTGGC+AGG - chr8_1:63716162-63716181 None:intergenic 70.0%
GCAGGCCTGGCACTGTTGGC+AGG - chr8_1:63716162-63716181 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 63714252 63716590 63714252 ID=Msa1206650;Name=Msa1206650
chr8_1 mRNA 63714252 63716590 63714252 ID=Msa1206650-mRNA-1;Parent=Msa1206650;Name=Msa1206650-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|1|0.85|1|1|1|7|351|207
chr8_1 exon 63714252 63714353 63714252 ID=Msa1206650-mRNA-1:exon:20761;Parent=Msa1206650-mRNA-1
chr8_1 exon 63714828 63714875 63714828 ID=Msa1206650-mRNA-1:exon:20762;Parent=Msa1206650-mRNA-1
chr8_1 exon 63714967 63715014 63714967 ID=Msa1206650-mRNA-1:exon:20763;Parent=Msa1206650-mRNA-1
chr8_1 exon 63715464 63715535 63715464 ID=Msa1206650-mRNA-1:exon:20764;Parent=Msa1206650-mRNA-1
chr8_1 exon 63715630 63715785 63715630 ID=Msa1206650-mRNA-1:exon:20765;Parent=Msa1206650-mRNA-1
chr8_1 exon 63715900 63716003 63715900 ID=Msa1206650-mRNA-1:exon:20766;Parent=Msa1206650-mRNA-1
chr8_1 exon 63716146 63716590 63716146 ID=Msa1206650-mRNA-1:exon:20767;Parent=Msa1206650-mRNA-1
chr8_1 CDS 63714252 63714353 63714252 ID=Msa1206650-mRNA-1:cds;Parent=Msa1206650-mRNA-1
chr8_1 CDS 63714828 63714875 63714828 ID=Msa1206650-mRNA-1:cds;Parent=Msa1206650-mRNA-1
chr8_1 CDS 63714967 63715014 63714967 ID=Msa1206650-mRNA-1:cds;Parent=Msa1206650-mRNA-1
chr8_1 CDS 63715464 63715535 63715464 ID=Msa1206650-mRNA-1:cds;Parent=Msa1206650-mRNA-1
chr8_1 CDS 63715630 63715785 63715630 ID=Msa1206650-mRNA-1:cds;Parent=Msa1206650-mRNA-1
chr8_1 CDS 63715900 63716003 63715900 ID=Msa1206650-mRNA-1:cds;Parent=Msa1206650-mRNA-1
chr8_1 CDS 63716146 63716239 63716146 ID=Msa1206650-mRNA-1:cds;Parent=Msa1206650-mRNA-1
chr8_1 three_prime_UTR 63716240 63716590 63716240 ID=Msa1206650-mRNA-1:three_prime_utr;Parent=Msa1206650-mRNA-1
Gene Sequence

>Msa1206650

TTGATTGGGGCATCCTATCTTATTTTCAGTGACTATTTGTTCAAGCTTTTGTTGATTGGAGATTCTGGTGTGGGCAAGTCATGTCTCCTCCTGAGGTTTGCTGATGATTCATACCTTGACAGCTATATCAGTACCATCGGAGTGGACTTTAAAATCCGCACTGTTGAGCAGGATGGGAAGACCATTAAACTTCAAATTTGGGACACTGCTGGTCAAGAACGTTTCCGGACTATAACTAGCAGCTACTATCGTGGGGCTCATGGCATAATTGTTGTTTACGATGTCACTGACCAAGAGAGCTTTAACAATGTTAAGCAGTGGCTGAATGAAATTGACCGCTATGCAAGTGAAAATGTTAACAAGCTTCTAGTTGGAAACAAGAGCGATCTCTCGGACAAAAAAGTTGTGTCCTCTGAAACAGCAAAGGCATTTGCAGATGAAATTGGAATTCCTTTCATGGAAACTAGTGCTAAAAATGCCTCCAATGTGGAACAAGCTTTCATGGCCATGGCTGCTGAAATTAAAAACAGAATGGCGAGTCAACCTGCCAACAGTGCCAGGCCTGCAACAGTCCAAATTCGAGGACAACCAGTGAACCAGAAGGCTGGTTGCTGCTCGTCTTAG
Protein sequence

>Msa1206650

LIGASYLIFSDYLFKLLLIGDSGVGKSCLLLRFADDSYLDSYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNKSDLSDKKVVSSETAKAFADEIGIPFMETSAKNASNVEQAFMAMAAEIKNRMASQPANSARPATVQIRGQPVNQKAGCCSS*