AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1390100


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 21 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAACACTGAGAAGCGAAAA+TGG 0.300690 tig0021331:+56615 Msa1390100:CDS
AAGAAGAAGCAAGGTGTAGA+TGG 0.344753 tig0021331:-56684 None:intergenic
CATACGAGCTCATCCCTTCA+AGG 0.437361 tig0021331:-57917 None:intergenic
AACGAGGTTCCTAACCTTGA+AGG 0.452298 tig0021331:+57903 Msa1390100:CDS
GAGCTCATCCCTTCAAGGTT+AGG 0.453636 tig0021331:-57912 None:intergenic
ATAGGAATCATCTTAGAGAT+AGG 0.479459 tig0021331:+57991 Msa1390100:CDS
ATCATCTTAGAGATAGGATG+TGG 0.498557 tig0021331:+57997 Msa1390100:CDS
TTAACAAGGTTATCTGATGA+AGG 0.516699 tig0021331:+57948 Msa1390100:CDS
TGATGAAGGTGCAGCTGAGA+TGG 0.559464 tig0021331:+57962 Msa1390100:CDS
CAGCTGAGATGGCAATGGAT+AGG 0.572746 tig0021331:+57973 Msa1390100:CDS
CGTATGTTGAAAACTTAACA+AGG 0.583345 tig0021331:+57934 Msa1390100:CDS
AGCGAAAATGGCGATTTCGA+CGG 0.590473 tig0021331:+56627 Msa1390100:CDS
ACGAGGTTCCTAACCTTGAA+GGG 0.611095 tig0021331:+57904 Msa1390100:CDS
AAAACTTGAGGCGGTGAACG+AGG 0.615754 tig0021331:+57887 Msa1390100:intron
ACCGTGCTTCTTCTTCTCTG+CGG 0.616187 tig0021331:+56727 Msa1390100:CDS
ACCGCAGAGAAGAAGAAGCA+CGG 0.624330 tig0021331:-56728 None:intergenic
AGGTGCAGCTGAGATGGCAA+TGG 0.647281 tig0021331:+57968 Msa1390100:CDS
TCATCTTAGAGATAGGATGT+GGG 0.679475 tig0021331:+57998 Msa1390100:CDS
GCGGTGGAGAAGAAGAAGCA+AGG 0.680210 tig0021331:-56693 None:intergenic
ACGGTAGAGAAGAAACGCGG+TGG 0.741441 tig0021331:-56709 None:intergenic
AGCACGGTAGAGAAGAAACG+CGG 0.793637 tig0021331:-56712 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGTTTTTTTTATCTAAAC+AGG - tig0021331:57064-57083 None:intergenic 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG - tig0021331:57400-57419 None:intergenic 15.0%
!!! TAGTTTTAAAAGTAATACTT+TGG - tig0021331:57503-57522 None:intergenic 15.0%
!! AAATTATCCTTATTAACTAT+TGG - tig0021331:57527-57546 None:intergenic 15.0%
!! AATAGTTAATAAGGATAATT+TGG + tig0021331:57526-57545 Msa1390100:intron 15.0%
!!! TAAGTTTTTTTTATCTAAAC+AGG - tig0021331:57064-57083 None:intergenic 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG - tig0021331:57400-57419 None:intergenic 15.0%
!!! TAGTTTTAAAAGTAATACTT+TGG - tig0021331:57503-57522 None:intergenic 15.0%
!! AAATTATCCTTATTAACTAT+TGG - tig0021331:57527-57546 None:intergenic 15.0%
!! AATAGTTAATAAGGATAATT+TGG + tig0021331:57526-57545 Msa1390100:intron 15.0%
!! AGTAAAAAAATCGCTATATT+AGG - tig0021331:56830-56849 None:intergenic 20.0%
!! AAAAACACAAAATCATAGAA+CGG - tig0021331:56882-56901 None:intergenic 20.0%
!!! TCTATGTAAATTTAAGATTG+CGG + tig0021331:56906-56925 Msa1390100:intron 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG - tig0021331:57440-57459 None:intergenic 20.0%
!! AAACTAACCAATAGTTAATA+AGG + tig0021331:57517-57536 Msa1390100:intron 20.0%
!! AATGATTAAGAGAAAGAAAA+TGG - tig0021331:57560-57579 None:intergenic 20.0%
!! TATACAAATACTAGATAAGT+AGG - tig0021331:57693-57712 None:intergenic 20.0%
!! TACTTATCTAGTATTTGTAT+AGG + tig0021331:57692-57711 Msa1390100:intron 20.0%
!! AGTAAAAAAATCGCTATATT+AGG - tig0021331:56830-56849 None:intergenic 20.0%
!! AAAAACACAAAATCATAGAA+CGG - tig0021331:56882-56901 None:intergenic 20.0%
!!! TCTATGTAAATTTAAGATTG+CGG + tig0021331:56906-56925 Msa1390100:intron 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG - tig0021331:57440-57459 None:intergenic 20.0%
!! AAACTAACCAATAGTTAATA+AGG + tig0021331:57517-57536 Msa1390100:intron 20.0%
!! AATGATTAAGAGAAAGAAAA+TGG - tig0021331:57560-57579 None:intergenic 20.0%
!! TATACAAATACTAGATAAGT+AGG - tig0021331:57693-57712 None:intergenic 20.0%
!! TACTTATCTAGTATTTGTAT+AGG + tig0021331:57692-57711 Msa1390100:intron 20.0%
! TGTATCTTTCAATATTCACA+CGG + tig0021331:57321-57340 Msa1390100:intron 25.0%
! TAGAGAAGTAAACAATCATA+AGG - tig0021331:57720-57739 None:intergenic 25.0%
!! TTATTGTTACTTAGTATGAC+AGG + tig0021331:57843-57862 Msa1390100:intron 25.0%
! TAACTGATATTGAAAACTTG+AGG + tig0021331:57875-57894 Msa1390100:intron 25.0%
! TGTATCTTTCAATATTCACA+CGG + tig0021331:57321-57340 Msa1390100:intron 25.0%
! TAGAGAAGTAAACAATCATA+AGG - tig0021331:57720-57739 None:intergenic 25.0%
!! TTATTGTTACTTAGTATGAC+AGG + tig0021331:57843-57862 Msa1390100:intron 25.0%
! TAACTGATATTGAAAACTTG+AGG + tig0021331:57875-57894 Msa1390100:intron 25.0%
!! TTAGTTTTTCTGCAGAATGA+GGG - tig0021331:56591-56610 None:intergenic 30.0%
TGTAGATGGAAGAAAAATCA+CGG - tig0021331:56673-56692 None:intergenic 30.0%
GATTGATATTTGCAGCTTAT+AGG + tig0021331:57025-57044 Msa1390100:intron 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG - tig0021331:57411-57430 None:intergenic 30.0%
!! AAACGACACTCATTTTGAAA+CGG + tig0021331:57609-57628 Msa1390100:intron 30.0%
CTCGATAAGAGGAAAATAAA+AGG - tig0021331:57806-57825 None:intergenic 30.0%
CGTATGTTGAAAACTTAACA+AGG + tig0021331:57934-57953 Msa1390100:CDS 30.0%
TTAACAAGGTTATCTGATGA+AGG + tig0021331:57948-57967 Msa1390100:CDS 30.0%
ATAGGAATCATCTTAGAGAT+AGG + tig0021331:57991-58010 Msa1390100:CDS 30.0%
!! TTAGTTTTTCTGCAGAATGA+GGG - tig0021331:56591-56610 None:intergenic 30.0%
TGTAGATGGAAGAAAAATCA+CGG - tig0021331:56673-56692 None:intergenic 30.0%
GATTGATATTTGCAGCTTAT+AGG + tig0021331:57025-57044 Msa1390100:intron 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG - tig0021331:57411-57430 None:intergenic 30.0%
!! AAACGACACTCATTTTGAAA+CGG + tig0021331:57609-57628 Msa1390100:intron 30.0%
CTCGATAAGAGGAAAATAAA+AGG - tig0021331:57806-57825 None:intergenic 30.0%
CGTATGTTGAAAACTTAACA+AGG + tig0021331:57934-57953 Msa1390100:CDS 30.0%
TTAACAAGGTTATCTGATGA+AGG + tig0021331:57948-57967 Msa1390100:CDS 30.0%
ATAGGAATCATCTTAGAGAT+AGG + tig0021331:57991-58010 Msa1390100:CDS 30.0%
!!! GTTAGTTTTTCTGCAGAATG+AGG - tig0021331:56592-56611 None:intergenic 35.0%
ATATTTGCAGCTTATAGGTC+TGG + tig0021331:57030-57049 Msa1390100:intron 35.0%
TTCAATATTCACACGGATAC+CGG + tig0021331:57328-57347 Msa1390100:intron 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - tig0021331:57374-57393 None:intergenic 35.0%
! TGTGCAGAGTATTAATTGGA+GGG - tig0021331:57474-57493 None:intergenic 35.0%
! GTAGTGTGCAGAGTATTAAT+TGG - tig0021331:57478-57497 None:intergenic 35.0%
AAAACACGATACTCGATAAG+AGG - tig0021331:57817-57836 None:intergenic 35.0%
CTGATATTGAAAACTTGAGG+CGG + tig0021331:57878-57897 Msa1390100:intron 35.0%
ATCATCTTAGAGATAGGATG+TGG + tig0021331:57997-58016 Msa1390100:CDS 35.0%
TCATCTTAGAGATAGGATGT+GGG + tig0021331:57998-58017 Msa1390100:CDS 35.0%
!!! GTTAGTTTTTCTGCAGAATG+AGG - tig0021331:56592-56611 None:intergenic 35.0%
ATATTTGCAGCTTATAGGTC+TGG + tig0021331:57030-57049 Msa1390100:intron 35.0%
TTCAATATTCACACGGATAC+CGG + tig0021331:57328-57347 Msa1390100:intron 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - tig0021331:57374-57393 None:intergenic 35.0%
! TGTGCAGAGTATTAATTGGA+GGG - tig0021331:57474-57493 None:intergenic 35.0%
! GTAGTGTGCAGAGTATTAAT+TGG - tig0021331:57478-57497 None:intergenic 35.0%
AAAACACGATACTCGATAAG+AGG - tig0021331:57817-57836 None:intergenic 35.0%
CTGATATTGAAAACTTGAGG+CGG + tig0021331:57878-57897 Msa1390100:intron 35.0%
ATCATCTTAGAGATAGGATG+TGG + tig0021331:57997-58016 Msa1390100:CDS 35.0%
TCATCTTAGAGATAGGATGT+GGG + tig0021331:57998-58017 Msa1390100:CDS 35.0%
AGAACACTGAGAAGCGAAAA+TGG + tig0021331:56615-56634 Msa1390100:CDS 40.0%
AGATGGAAGAAAAATCACGG+CGG - tig0021331:56670-56689 None:intergenic 40.0%
AAGAAGAAGCAAGGTGTAGA+TGG - tig0021331:56687-56706 None:intergenic 40.0%
!!! TTCTTCTCTGCGGTTTTTTC+AGG + tig0021331:56737-56756 Msa1390100:CDS 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - tig0021331:57613-57632 None:intergenic 40.0%
! GTGTGCAGAGTATTAATTGG+AGG - tig0021331:57475-57494 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + tig0021331:57613-57632 Msa1390100:intron 40.0%
!! AACGGAGGGAGTATTTGTTT+AGG + tig0021331:57627-57646 Msa1390100:intron 40.0%
AGAACACTGAGAAGCGAAAA+TGG + tig0021331:56615-56634 Msa1390100:CDS 40.0%
AGATGGAAGAAAAATCACGG+CGG - tig0021331:56670-56689 None:intergenic 40.0%
AAGAAGAAGCAAGGTGTAGA+TGG - tig0021331:56687-56706 None:intergenic 40.0%
!!! TTCTTCTCTGCGGTTTTTTC+AGG + tig0021331:56737-56756 Msa1390100:CDS 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - tig0021331:57613-57632 None:intergenic 40.0%
! GTGTGCAGAGTATTAATTGG+AGG - tig0021331:57475-57494 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + tig0021331:57613-57632 Msa1390100:intron 40.0%
!! AACGGAGGGAGTATTTGTTT+AGG + tig0021331:57627-57646 Msa1390100:intron 40.0%
AGCGAAAATGGCGATTTCGA+CGG + tig0021331:56627-56646 Msa1390100:CDS 45.0%
AGAAAAATCACGGCGGAGAT+CGG - tig0021331:56663-56682 None:intergenic 45.0%
!! GGGAGTACAGGTTTTGTATC+CGG - tig0021331:57350-57369 None:intergenic 45.0%
!!! TTTTGAAACGGAGGGAGTAC+AGG - tig0021331:57362-57381 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - tig0021331:57612-57631 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG + tig0021331:57612-57631 Msa1390100:intron 45.0%
AACGAGGTTCCTAACCTTGA+AGG + tig0021331:57903-57922 Msa1390100:CDS 45.0%
ACGAGGTTCCTAACCTTGAA+GGG + tig0021331:57904-57923 Msa1390100:CDS 45.0%
AGCGAAAATGGCGATTTCGA+CGG + tig0021331:56627-56646 Msa1390100:CDS 45.0%
AGAAAAATCACGGCGGAGAT+CGG - tig0021331:56663-56682 None:intergenic 45.0%
!! GGGAGTACAGGTTTTGTATC+CGG - tig0021331:57350-57369 None:intergenic 45.0%
!!! TTTTGAAACGGAGGGAGTAC+AGG - tig0021331:57362-57381 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - tig0021331:57612-57631 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG + tig0021331:57612-57631 Msa1390100:intron 45.0%
AACGAGGTTCCTAACCTTGA+AGG + tig0021331:57903-57922 Msa1390100:CDS 45.0%
ACGAGGTTCCTAACCTTGAA+GGG + tig0021331:57904-57923 Msa1390100:CDS 45.0%
AGCACGGTAGAGAAGAAACG+CGG - tig0021331:56715-56734 None:intergenic 50.0%
ACCGTGCTTCTTCTTCTCTG+CGG + tig0021331:56727-56746 Msa1390100:CDS 50.0%
ACCGCAGAGAAGAAGAAGCA+CGG - tig0021331:56731-56750 None:intergenic 50.0%
AAAACTTGAGGCGGTGAACG+AGG + tig0021331:57887-57906 Msa1390100:intron 50.0%
GAGCTCATCCCTTCAAGGTT+AGG - tig0021331:57915-57934 None:intergenic 50.0%
CATACGAGCTCATCCCTTCA+AGG - tig0021331:57920-57939 None:intergenic 50.0%
! TGATGAAGGTGCAGCTGAGA+TGG + tig0021331:57962-57981 Msa1390100:CDS 50.0%
CAGCTGAGATGGCAATGGAT+AGG + tig0021331:57973-57992 Msa1390100:CDS 50.0%
AGCACGGTAGAGAAGAAACG+CGG - tig0021331:56715-56734 None:intergenic 50.0%
ACCGTGCTTCTTCTTCTCTG+CGG + tig0021331:56727-56746 Msa1390100:CDS 50.0%
ACCGCAGAGAAGAAGAAGCA+CGG - tig0021331:56731-56750 None:intergenic 50.0%
AAAACTTGAGGCGGTGAACG+AGG + tig0021331:57887-57906 Msa1390100:intron 50.0%
GAGCTCATCCCTTCAAGGTT+AGG - tig0021331:57915-57934 None:intergenic 50.0%
CATACGAGCTCATCCCTTCA+AGG - tig0021331:57920-57939 None:intergenic 50.0%
! TGATGAAGGTGCAGCTGAGA+TGG + tig0021331:57962-57981 Msa1390100:CDS 50.0%
CAGCTGAGATGGCAATGGAT+AGG + tig0021331:57973-57992 Msa1390100:CDS 50.0%
GCGGTGGAGAAGAAGAAGCA+AGG - tig0021331:56696-56715 None:intergenic 55.0%
ACGGTAGAGAAGAAACGCGG+TGG - tig0021331:56712-56731 None:intergenic 55.0%
AGGTGCAGCTGAGATGGCAA+TGG + tig0021331:57968-57987 Msa1390100:CDS 55.0%
GCGGTGGAGAAGAAGAAGCA+AGG - tig0021331:56696-56715 None:intergenic 55.0%
ACGGTAGAGAAGAAACGCGG+TGG - tig0021331:56712-56731 None:intergenic 55.0%
AGGTGCAGCTGAGATGGCAA+TGG + tig0021331:57968-57987 Msa1390100:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0021331 gene 56567 58037 56567 ID=Msa1390100;Name=Msa1390100
tig0021331 mRNA 56567 58037 56567 ID=Msa1390100-mRNA-1;Parent=Msa1390100;Name=Msa1390100-mRNA-1;_AED=0.16;_eAED=0.52;_QI=0|0|0|1|1|1|2|0|110
tig0021331 exon 56567 56758 56567 ID=Msa1390100-mRNA-1:exon:11994;Parent=Msa1390100-mRNA-1
tig0021331 exon 57897 58037 57897 ID=Msa1390100-mRNA-1:exon:11995;Parent=Msa1390100-mRNA-1
tig0021331 CDS 56567 56758 56567 ID=Msa1390100-mRNA-1:cds;Parent=Msa1390100-mRNA-1
tig0021331 CDS 57897 58037 57897 ID=Msa1390100-mRNA-1:cds;Parent=Msa1390100-mRNA-1
Gene Sequence

>Msa1390100

AAAAATTGGATCAAAAATTATCCCTCATTCTGCAGAAAAACTAACGTGAGAACACTGAGAAGCGAAAATGGCGATTTCGACGGCGATTCTTCTCCGATCTCCGCCGTGATTTTTCTTCCATCTACACCTTGCTTCTTCTTCTCCACCGCGTTTCTTCTCTACCGTGCTTCTTCTTCTCTGCGGTTTTTTCAGGCGGTGAACGAGGTTCCTAACCTTGAAGGGATGAGCTCGTATGTTGAAAACTTAACAAGGTTATCTGATGAAGGTGCAGCTGAGATGGCAATGGATAGGAATCATCTTAGAGATAGGATGTGGGTACATCGTCATAATTAA

Protein sequence

>Msa1390100

KNWIKNYPSFCRKTNVRTLRSENGDFDGDSSPISAVIFLPSTPCFFFSTAFLLYRASSSLRFFQAVNEVPNLEGMSSYVENLTRLSDEGAAEMAMDRNHLRDRMWVHRHN*