AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene002746

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002746.t1 MTR_2g019670 99.338 151 1 0 1 151 1 151 1.12e-107 303
MS.gene002746.t1 MTR_4g096790 99.338 151 1 0 1 151 1 151 5.68e-107 301
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002746.t1 AT3G11510 89.855 138 14 0 3 140 2 139 8.87e-90 258
MS.gene002746.t1 AT3G52580 89.130 138 15 0 3 140 2 139 2.75e-89 256
MS.gene002746.t1 AT2G36160 88.406 138 16 0 3 140 2 139 1.64e-88 254
MS.gene002746.t1 ATCG00750 33.613 119 70 2 29 147 28 137 7.46e-12 59.7

Find 51 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCGTGCCCTTGCTCGTTC+TGG 0.240493 2.1:+67494645 MS.gene002746:CDS
AGCACCAGGGCCAGGTGTTT+TGG 0.248968 2.1:-67494613 None:intergenic
GCTCGTTCTGGAATGAAAAT+TGG 0.299156 2.1:+67494657 MS.gene002746:CDS
AATGTTACTGTCTCTTCCTT+TGG 0.327634 2.1:-67493258 None:intergenic
CCCTTCTACCACTCTTTCTA+CGG 0.354483 2.1:-67495668 None:intergenic
AGAGTGGTAGAAGGGGTAGA+AGG 0.383998 2.1:+67495676 MS.gene002746:CDS
AACATTGGGTCCAGCTGTTA+AGG 0.388167 2.1:+67493275 MS.gene002746:CDS
CGAAGAGCAGCTTGAGCACC+AGG 0.399712 2.1:-67494627 None:intergenic
AGTCGTCAAAGAGAAAGGTT+AGG 0.404664 2.1:+67493232 MS.gene002746:intron
TGCCGCTATGCTTGCAGCAC+AGG 0.424923 2.1:+67494280 MS.gene002746:CDS
TTTAGGTGGGATGAAAGTTA+AGG 0.429174 2.1:+67494232 MS.gene002746:intron
GGAATGAAAATTGGTCGTAT+AGG 0.435498 2.1:+67494666 MS.gene002746:CDS
GCAGCTTGAGCACCAGGGCC+AGG 0.435589 2.1:-67494621 None:intergenic
CAGCATGTTACTGATTTATC+CGG 0.442376 2.1:+67493449 MS.gene002746:intron
TGCTGCAAGCATAGCGGCAT+AGG 0.460137 2.1:-67494276 None:intergenic
GTGATAGTCGTCAAAGAGAA+AGG 0.460831 2.1:+67493227 MS.gene002746:intron
CACATTAAGCTCCGTGCCAC+TGG 0.465038 2.1:+67494579 MS.gene002746:CDS
CCCGTAGAAAGAGTGGTAGA+AGG 0.472164 2.1:+67495667 MS.gene002746:CDS
GAAACCCTCGTCCGTATCAC+TGG 0.498735 2.1:+67493476 MS.gene002746:CDS
TTTGGTCTTGTTTCCACCAG+TGG 0.501009 2.1:-67494595 None:intergenic
ACGGGTGCTATCGGAAGGAA+TGG 0.504362 2.1:-67495649 None:intergenic
TGTTCACCATCCTTAACAGC+TGG 0.531081 2.1:-67493285 None:intergenic
ACTCTTTCTACGGGTGCTAT+CGG 0.532615 2.1:-67495658 None:intergenic
CCGTAGAAAGAGTGGTAGAA+GGG 0.533450 2.1:+67495668 MS.gene002746:CDS
AAAGGAAGAGACAGTAACAT+TGG 0.536923 2.1:+67493260 MS.gene002746:CDS
CCTTCTACCACTCTTTCTAC+GGG 0.539502 2.1:-67495667 None:intergenic
CATATATATACTAACAATGA+AGG 0.543037 2.1:-67493349 None:intergenic
ATGTTACTGATTTATCCGGC+AGG 0.543996 2.1:+67493453 MS.gene002746:CDS
ACGGACGAGGGTTTCCCTGC+CGG 0.547105 2.1:-67493468 None:intergenic
GTCGTCAAAGAGAAAGGTTA+GGG 0.549356 2.1:+67493233 MS.gene002746:intron
AAGACCAAAACACCTGGCCC+TGG 0.552706 2.1:+67494609 MS.gene002746:CDS
AAGGAAGAGACAGTAACATT+GGG 0.554128 2.1:+67493261 MS.gene002746:CDS
TCTTGTTTCCACCAGTGGCA+CGG 0.555351 2.1:-67494590 None:intergenic
GAGAAAGGTTAGGGAGCCAA+AGG 0.560093 2.1:+67493242 MS.gene002746:CDS
ACTCATACATACCAGTGATA+CGG 0.570439 2.1:-67493487 None:intergenic
ATTAAGCTCCGTGCCACTGG+TGG 0.571382 2.1:+67494582 MS.gene002746:CDS
GCTGCAAGCATAGCGGCATA+GGG 0.578463 2.1:-67494275 None:intergenic
TTTCATTCCAGAACGAGCAA+GGG 0.582609 2.1:-67494652 None:intergenic
ACATACCAGTGATACGGACG+AGG 0.584850 2.1:-67493481 None:intergenic
CGGGTGCTATCGGAAGGAAT+GGG 0.585535 2.1:-67495648 None:intergenic
GGAAACAAGACCAAAACACC+TGG 0.596535 2.1:+67494603 MS.gene002746:CDS
TTTCTACGGGTGCTATCGGA+AGG 0.607952 2.1:-67495654 None:intergenic
TGTTACTGATTTATCCGGCA+GGG 0.618792 2.1:+67493454 MS.gene002746:CDS
TTGGGTCCAGCTGTTAAGGA+TGG 0.623308 2.1:+67493279 MS.gene002746:CDS
CGTAGAAAGAGTGGTAGAAG+GGG 0.630512 2.1:+67495669 MS.gene002746:CDS
GATAGCACCCGTAGAAAGAG+TGG 0.631829 2.1:+67495660 MS.gene002746:CDS
GGATGTTGCTACTAGATGCA+AGG 0.645225 2.1:+67494301 MS.gene002746:CDS
ACGGAGCTTAATGTGAAGTG+CGG 0.698863 2.1:-67494571 None:intergenic
ATCCTGTGCTGCAAGCATAG+CGG 0.706099 2.1:-67494282 None:intergenic
GAAGAGCAGCTTGAGCACCA+GGG 0.706692 2.1:-67494626 None:intergenic
CATACCAGTGATACGGACGA+GGG 0.727503 2.1:-67493480 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTAAAAAATTACATATTTA+AGG + chr2.1:67493587-67493606 MS.gene002746:intron 10.0%
!!! AGCATTATTTTTTTTTTATA+TGG + chr2.1:67494490-67494509 MS.gene002746:intron 10.0%
!!! CATTATTTTTTTTTTATATG+GGG + chr2.1:67494492-67494511 MS.gene002746:intron 10.0%
!!! GCATTATTTTTTTTTTATAT+GGG + chr2.1:67494491-67494510 MS.gene002746:intron 10.0%
!!! TTATAAGTTTTTAATTTAGA+TGG + chr2.1:67494089-67494108 MS.gene002746:intron 10.0%
!! ATTTGATAAGTTAATTTAGA+TGG + chr2.1:67494033-67494052 MS.gene002746:intron 15.0%
!! TTTGAAAAACCTTAAAAATT+TGG + chr2.1:67493620-67493639 MS.gene002746:intron 15.0%
!!! TACAAATAACCAAATTTTTA+AGG - chr2.1:67493632-67493651 None:intergenic 15.0%
!!! TTAGTTACTATCATATTTTA+AGG + chr2.1:67493555-67493574 MS.gene002746:intron 15.0%
!!! TTGTTTTTTTTCCAAATTTT+AGG + chr2.1:67494215-67494234 MS.gene002746:intron 15.0%
!! AATATTCACTATAGTACATA+TGG + chr2.1:67495035-67495054 MS.gene002746:intron 20.0%
!! ATTGAAAAATAATTATTCCC+GGG - chr2.1:67493950-67493969 None:intergenic 20.0%
!! CATATATATACTAACAATGA+AGG - chr2.1:67493352-67493371 None:intergenic 20.0%
!! GATAATTGTATTTAATCTGA+TGG - chr2.1:67494824-67494843 None:intergenic 20.0%
!! GATTGAAAAATAATTATTCC+CGG - chr2.1:67493951-67493970 None:intergenic 20.0%
!! TATTGTATAGAATAACTGTA+AGG + chr2.1:67494952-67494971 MS.gene002746:intron 20.0%
!!! GAATTTTTTTTAAAATACCC+TGG - chr2.1:67493804-67493823 None:intergenic 20.0%
!!! TGAATGTTTTTGTTTTGTTT+TGG + chr2.1:67494451-67494470 MS.gene002746:intron 20.0%
!!! TGCTCTTATTTTAAGTTATT+TGG + chr2.1:67495066-67495085 MS.gene002746:intron 20.0%
!!! TGTACTGATTCAAAATATTT+TGG + chr2.1:67494530-67494549 MS.gene002746:intron 20.0%
!!! TTCAAGTTATTTGAGTTATT+TGG + chr2.1:67494897-67494916 MS.gene002746:intron 20.0%
!!! TTTTTTTCCAAATTTTAGGT+GGG + chr2.1:67494219-67494238 MS.gene002746:intron 20.0%
!!! TTTTTTTTCCAAATTTTAGG+TGG + chr2.1:67494218-67494237 MS.gene002746:intron 20.0%
! AATTGAAAAAATTCCTAGTC+TGG - chr2.1:67495471-67495490 None:intergenic 25.0%
! ACAAAATTTAATGGGAATGT+TGG + chr2.1:67493885-67493904 MS.gene002746:intron 25.0%
! AGTTATAGTTAAGAGAAACA+TGG + chr2.1:67495007-67495026 MS.gene002746:intron 25.0%
! ATATTTACGATGTGAAAGTT+AGG + chr2.1:67494760-67494779 MS.gene002746:intron 25.0%
! CAAAATTTAATGGGAATGTT+GGG + chr2.1:67493886-67493905 MS.gene002746:intron 25.0%
! GAATAATTAATTTCACTGAG+AGG - chr2.1:67495539-67495558 None:intergenic 25.0%
! GCGTATTGTATAGAATTATT+TGG + chr2.1:67494923-67494942 MS.gene002746:intron 25.0%
! TAACTCTCTATAATTGTTCT+TGG - chr2.1:67493660-67493679 None:intergenic 25.0%
! TAATAGGTAAAAGAGAATCA+GGG + chr2.1:67495163-67495182 MS.gene002746:intron 25.0%
! TTCAATCCTGTAACAAATAT+GGG + chr2.1:67493964-67493983 MS.gene002746:intron 25.0%
! TTTCAATCCTGTAACAAATA+TGG + chr2.1:67493963-67493982 MS.gene002746:intron 25.0%
!! AAATAAATTCCAGTTTTCCT+AGG - chr2.1:67493848-67493867 None:intergenic 25.0%
!! TGTTAGGATTTTTATCTTTG+TGG + chr2.1:67493708-67493727 MS.gene002746:intron 25.0%
!!! GAATTGCATGTTTTAATTAC+TGG + chr2.1:67494168-67494187 MS.gene002746:intron 25.0%
AAACCTTAAACTATCTTCTG+TGG - chr2.1:67494728-67494747 None:intergenic 30.0%
AACCTTAAACTATCTTCTGT+GGG - chr2.1:67494727-67494746 None:intergenic 30.0%
ACTGTGTTTACACTGTTAAA+TGG + chr2.1:67494409-67494428 MS.gene002746:intron 30.0%
AGCATGATTATTCTATACTC+TGG + chr2.1:67493681-67493700 MS.gene002746:intron 30.0%
ATAATTATTCCCGGGTATAA+AGG - chr2.1:67493942-67493961 None:intergenic 30.0%
ATCTGTACTGTATTGAACAA+CGG + chr2.1:67495090-67495109 MS.gene002746:intron 30.0%
GTAATAGGTAAAAGAGAATC+AGG + chr2.1:67495162-67495181 MS.gene002746:intron 30.0%
TAAGTATATTCTCTCTCTTC+TGG - chr2.1:67494876-67494895 None:intergenic 30.0%
TTGAATATTCCTAGGAAAAC+TGG + chr2.1:67493836-67493855 MS.gene002746:intron 30.0%
TTTCTCTTAACTATAACTGC+TGG - chr2.1:67495005-67495024 None:intergenic 30.0%
! AACTGACTCAAATTATTCCT+AGG + chr2.1:67493740-67493759 MS.gene002746:intron 30.0%
! AGTATACCCATATTTGTTAC+AGG - chr2.1:67493973-67493992 None:intergenic 30.0%
! ATGTTGCTGTTGATATTGTT+TGG + chr2.1:67494141-67494160 MS.gene002746:intron 30.0%
! GTTTTTGTCAAAACACTCTT+AGG - chr2.1:67494001-67494020 None:intergenic 30.0%
! TCAAACCAAAATGCGTTTTT+GGG + chr2.1:67494376-67494395 MS.gene002746:intron 30.0%
!! TTTTTATGTTGCTATTGCAG+AGG + chr2.1:67495617-67495636 MS.gene002746:intron 30.0%
!!! CATGTTTTAATTACTGGATG+TGG + chr2.1:67494174-67494193 MS.gene002746:intron 30.0%
!!! GATTCAAAATATTTTGGAGC+AGG + chr2.1:67494536-67494555 MS.gene002746:intron 30.0%
AAAGACAGTAGTTTGAATCC+AGG + chr2.1:67493783-67493802 MS.gene002746:intron 35.0%
AAAGAGAATCAGGGATATCT+AGG + chr2.1:67495172-67495191 MS.gene002746:intron 35.0%
AAAGGAAGAGACAGTAACAT+TGG + chr2.1:67493260-67493279 MS.gene002746:CDS 35.0%
AACCCACAGAAGATAGTTTA+AGG + chr2.1:67494722-67494741 MS.gene002746:intron 35.0%
AAGACAGTAGTTTGAATCCA+GGG + chr2.1:67493784-67493803 MS.gene002746:intron 35.0%
AAGGAAGAGACAGTAACATT+GGG + chr2.1:67493261-67493280 MS.gene002746:CDS 35.0%
AATGTTACTGTCTCTTCCTT+TGG - chr2.1:67493261-67493280 None:intergenic 35.0%
ACCAGAAAATCCAAATGTCT+TGG - chr2.1:67495280-67495299 None:intergenic 35.0%
ACTCATACATACCAGTGATA+CGG - chr2.1:67493490-67493509 None:intergenic 35.0%
ACTGAGTACGATATGGTAAT+AGG + chr2.1:67495147-67495166 MS.gene002746:intron 35.0%
ATTTCACTGAGAGGAAGATA+AGG - chr2.1:67495530-67495549 None:intergenic 35.0%
CAGCAACATGAATGTATCTT+AGG - chr2.1:67494130-67494149 None:intergenic 35.0%
CCAGAAAATCCAAATGTCTT+GGG - chr2.1:67495279-67495298 None:intergenic 35.0%
CTAGGAAAATATGTTGCACA+AGG + chr2.1:67495190-67495209 MS.gene002746:intron 35.0%
CTTTCATCCCACCTAAAATT+TGG - chr2.1:67494229-67494248 None:intergenic 35.0%
GCTGTTGATATTGTTTGGTT+TGG + chr2.1:67494146-67494165 MS.gene002746:intron 35.0%
GGAATGAAAATTGGTCGTAT+AGG + chr2.1:67494666-67494685 MS.gene002746:CDS 35.0%
TATCGTACTCAGTTCATCAA+CGG - chr2.1:67495140-67495159 None:intergenic 35.0%
TCTCTCTTCTGGTAAAATAG+AGG - chr2.1:67494865-67494884 None:intergenic 35.0%
TTCACAAGAACCTTTATACC+CGG + chr2.1:67493929-67493948 MS.gene002746:intron 35.0%
TTCTAGTGTATGTTGTTCCT+AGG - chr2.1:67493760-67493779 None:intergenic 35.0%
TTGATGAACTGAGTACGATA+TGG + chr2.1:67495140-67495159 MS.gene002746:intron 35.0%
TTTAGGTGGGATGAAAGTTA+AGG + chr2.1:67494232-67494251 MS.gene002746:intron 35.0%
TTTCAGTCTAATCCACGAAA+GGG + chr2.1:67495434-67495453 MS.gene002746:intron 35.0%
! AAGACATTTGGATTTTCTGG+TGG + chr2.1:67495279-67495298 MS.gene002746:intron 35.0%
! AAGGATGGTGAACATGTTTT+CGG + chr2.1:67493294-67493313 MS.gene002746:CDS 35.0%
! GTCAAACCAAAATGCGTTTT+TGG + chr2.1:67494375-67494394 MS.gene002746:intron 35.0%
! TCAACGGTGAACCTTATTTA+CGG - chr2.1:67495124-67495143 None:intergenic 35.0%
! TCTATACTCTGGTTTGTGTT+AGG + chr2.1:67493692-67493711 MS.gene002746:intron 35.0%
! TTTCACCCAAAAACGCATTT+TGG - chr2.1:67494384-67494403 None:intergenic 35.0%
!! CAGCATGTTACTGATTTATC+CGG + chr2.1:67493449-67493468 MS.gene002746:intron 35.0%
!! TTTCGTGGATTAGACTGAAA+CGG - chr2.1:67495434-67495453 None:intergenic 35.0%
ACAGGATTTAGCAAGCAAAC+AGG - chr2.1:67495406-67495425 None:intergenic 40.0%
AGTCGTCAAAGAGAAAGGTT+AGG + chr2.1:67493232-67493251 MS.gene002746:intron 40.0%
ATCCAAATGTCTTGGGACTA+AGG - chr2.1:67495272-67495291 None:intergenic 40.0%
ATCCTTAGTCCCAAGACATT+TGG + chr2.1:67495267-67495286 MS.gene002746:intron 40.0%
ATTTAGCAAGCAAACAGGGA+AGG - chr2.1:67495401-67495420 None:intergenic 40.0%
CAGGATTTAGCAAGCAAACA+GGG - chr2.1:67495405-67495424 None:intergenic 40.0%
CATCGTAAATATCAGAGAGC+AGG - chr2.1:67494752-67494771 None:intergenic 40.0%
CCACACTCTTGAATATTCCT+AGG + chr2.1:67493828-67493847 MS.gene002746:intron 40.0%
CCTAGGAATATTCAAGAGTG+TGG - chr2.1:67493831-67493850 None:intergenic 40.0%
GGATAGACACTTCATCTCTA+TGG - chr2.1:67495251-67495270 None:intergenic 40.0%
GTCGTCAAAGAGAAAGGTTA+GGG + chr2.1:67493233-67493252 MS.gene002746:intron 40.0%
GTGATAGTCGTCAAAGAGAA+AGG + chr2.1:67493227-67493246 MS.gene002746:intron 40.0%
GTTTCAGTCTAATCCACGAA+AGG + chr2.1:67495433-67495452 MS.gene002746:intron 40.0%
TAGACTGAAACGGTGATTAC+AGG - chr2.1:67495424-67495443 None:intergenic 40.0%
TCACAAGAACCTTTATACCC+GGG + chr2.1:67493930-67493949 MS.gene002746:intron 40.0%
TTTCATTCCAGAACGAGCAA+GGG - chr2.1:67494655-67494674 None:intergenic 40.0%
! CCCAAGACATTTGGATTTTC+TGG + chr2.1:67495276-67495295 MS.gene002746:intron 40.0%
! TCTACCACGTGAGTTCTTTT+TGG - chr2.1:67495347-67495366 None:intergenic 40.0%
! TTTTCATTCCAGAACGAGCA+AGG - chr2.1:67494656-67494675 None:intergenic 40.0%
!! ACATTTGGATTTTCTGGTGG+TGG + chr2.1:67495282-67495301 MS.gene002746:intron 40.0%
!! ATGTTACTGATTTATCCGGC+AGG + chr2.1:67493453-67493472 MS.gene002746:CDS 40.0%
!! GCTCGTTCTGGAATGAAAAT+TGG + chr2.1:67494657-67494676 MS.gene002746:CDS 40.0%
!! TGTTACTGATTTATCCGGCA+GGG + chr2.1:67493454-67493473 MS.gene002746:CDS 40.0%
!!! AATATTTTGGAGCAGGAGCT+CGG + chr2.1:67494543-67494562 MS.gene002746:intron 40.0%
AACATTGGGTCCAGCTGTTA+AGG + chr2.1:67493275-67493294 MS.gene002746:CDS 45.0%
ACGGAGCTTAATGTGAAGTG+CGG - chr2.1:67494574-67494593 None:intergenic 45.0%
ACTGAGAGGAAGATAAGGTC+AGG - chr2.1:67495525-67495544 None:intergenic 45.0%
CAGTCCAAAAAGAACTCACG+TGG + chr2.1:67495340-67495359 MS.gene002746:intron 45.0%
CCCTTCTACCACTCTTTCTA+CGG - chr2.1:67495671-67495690 None:intergenic 45.0%
CCGTAGAAAGAGTGGTAGAA+GGG + chr2.1:67495668-67495687 MS.gene002746:CDS 45.0%
CCTTCTACCACTCTTTCTAC+GGG - chr2.1:67495670-67495689 None:intergenic 45.0%
CGGTCGAATTGCCGTAAATA+AGG + chr2.1:67495110-67495129 MS.gene002746:intron 45.0%
CGTAGAAAGAGTGGTAGAAG+GGG + chr2.1:67495669-67495688 MS.gene002746:CDS 45.0%
CTGAGAGGAAGATAAGGTCA+GGG - chr2.1:67495524-67495543 None:intergenic 45.0%
GAAATGTGACAACCCTTTCG+TGG - chr2.1:67495449-67495468 None:intergenic 45.0%
GGAAACAAGACCAAAACACC+TGG + chr2.1:67494603-67494622 MS.gene002746:CDS 45.0%
GGATGTTGCTACTAGATGCA+AGG + chr2.1:67494301-67494320 MS.gene002746:CDS 45.0%
GGTTGTCACATTTCCAGACT+AGG + chr2.1:67495455-67495474 MS.gene002746:intron 45.0%
GTTGCTACTAGATGCAAGGT+CGG + chr2.1:67494305-67494324 MS.gene002746:intron 45.0%
TGTTCACCATCCTTAACAGC+TGG - chr2.1:67493288-67493307 None:intergenic 45.0%
! ACTCTTTCTACGGGTGCTAT+CGG - chr2.1:67495661-67495680 None:intergenic 45.0%
! CACGTGAGTTCTTTTTGGAC+TGG - chr2.1:67495342-67495361 None:intergenic 45.0%
!! TTTGGTCTTGTTTCCACCAG+TGG - chr2.1:67494598-67494617 None:intergenic 45.0%
!! AAAATTATACAAAATTTAAT+GGG + chr2.1:67493877-67493896 MS.gene002746:intron 5.0%
!! TAAAATTATACAAAATTTAA+TGG + chr2.1:67493876-67493895 MS.gene002746:intron 5.0%
ACATACCAGTGATACGGACG+AGG - chr2.1:67493484-67493503 None:intergenic 50.0%
AGAGTGGTAGAAGGGGTAGA+AGG + chr2.1:67495676-67495695 MS.gene002746:CDS 50.0%
ATCCTGTGCTGCAAGCATAG+CGG - chr2.1:67494285-67494304 None:intergenic 50.0%
CATACCAGTGATACGGACGA+GGG - chr2.1:67493483-67493502 None:intergenic 50.0%
CCCGTAGAAAGAGTGGTAGA+AGG + chr2.1:67495667-67495686 MS.gene002746:CDS 50.0%
GAGAAAGGTTAGGGAGCCAA+AGG + chr2.1:67493242-67493261 MS.gene002746:CDS 50.0%
GATAGCACCCGTAGAAAGAG+TGG + chr2.1:67495660-67495679 MS.gene002746:CDS 50.0%
TTGGGTCCAGCTGTTAAGGA+TGG + chr2.1:67493279-67493298 MS.gene002746:CDS 50.0%
! TCTTGTTTCCACCAGTGGCA+CGG - chr2.1:67494593-67494612 None:intergenic 50.0%
! TTTCTACGGGTGCTATCGGA+AGG - chr2.1:67495657-67495676 None:intergenic 50.0%
!! TTTCTGGTGGTGGTTGCTGT+AGG + chr2.1:67495292-67495311 MS.gene002746:intron 50.0%
AAGACCAAAACACCTGGCCC+TGG + chr2.1:67494609-67494628 MS.gene002746:CDS 55.0%
CACATTAAGCTCCGTGCCAC+TGG + chr2.1:67494579-67494598 MS.gene002746:CDS 55.0%
GAAACCCTCGTCCGTATCAC+TGG + chr2.1:67493476-67493495 MS.gene002746:CDS 55.0%
GAAGAGCAGCTTGAGCACCA+GGG - chr2.1:67494629-67494648 None:intergenic 55.0%
GCTACTAGATGCAAGGTCGG+TGG + chr2.1:67494308-67494327 MS.gene002746:intron 55.0%
GCTGCAAGCATAGCGGCATA+GGG - chr2.1:67494278-67494297 None:intergenic 55.0%
TGCTGCAAGCATAGCGGCAT+AGG - chr2.1:67494279-67494298 None:intergenic 55.0%
! ACGGGTGCTATCGGAAGGAA+TGG - chr2.1:67495652-67495671 None:intergenic 55.0%
! ATTAAGCTCCGTGCCACTGG+TGG + chr2.1:67494582-67494601 MS.gene002746:CDS 55.0%
! CGGGTGCTATCGGAAGGAAT+GGG - chr2.1:67495651-67495670 None:intergenic 55.0%
CGAAGAGCAGCTTGAGCACC+AGG - chr2.1:67494630-67494649 None:intergenic 60.0%
CTTCGTGCCCTTGCTCGTTC+TGG + chr2.1:67494645-67494664 MS.gene002746:CDS 60.0%
TGCCGCTATGCTTGCAGCAC+AGG + chr2.1:67494280-67494299 MS.gene002746:CDS 60.0%
! AGCACCAGGGCCAGGTGTTT+TGG - chr2.1:67494616-67494635 None:intergenic 60.0%
ACGGACGAGGGTTTCCCTGC+CGG - chr2.1:67493471-67493490 None:intergenic 65.0%
GCAGCTTGAGCACCAGGGCC+AGG - chr2.1:67494624-67494643 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 67493139 67495704 67493139 ID=MS.gene002746
chr2.1 mRNA 67493139 67495704 67493139 ID=MS.gene002746.t1;Parent=MS.gene002746
chr2.1 exon 67493139 67493141 67493139 ID=MS.gene002746.t1.exon1;Parent=MS.gene002746.t1
chr2.1 CDS 67493139 67493141 67493139 ID=cds.MS.gene002746.t1;Parent=MS.gene002746.t1
chr2.1 exon 67493234 67493356 67493234 ID=MS.gene002746.t1.exon2;Parent=MS.gene002746.t1
chr2.1 CDS 67493234 67493356 67493234 ID=cds.MS.gene002746.t1;Parent=MS.gene002746.t1
chr2.1 exon 67493452 67493497 67493452 ID=MS.gene002746.t1.exon3;Parent=MS.gene002746.t1
chr2.1 CDS 67493452 67493497 67493452 ID=cds.MS.gene002746.t1;Parent=MS.gene002746.t1
chr2.1 exon 67494237 67494322 67494237 ID=MS.gene002746.t1.exon4;Parent=MS.gene002746.t1
chr2.1 CDS 67494237 67494322 67494237 ID=cds.MS.gene002746.t1;Parent=MS.gene002746.t1
chr2.1 exon 67494558 67494687 67494558 ID=MS.gene002746.t1.exon5;Parent=MS.gene002746.t1
chr2.1 CDS 67494558 67494687 67494558 ID=cds.MS.gene002746.t1;Parent=MS.gene002746.t1
chr2.1 exon 67495637 67495704 67495637 ID=MS.gene002746.t1.exon6;Parent=MS.gene002746.t1
chr2.1 CDS 67495637 67495704 67495637 ID=cds.MS.gene002746.t1;Parent=MS.gene002746.t1
Gene Sequence

>MS.gene002746

ATGGTATCTTCATACGTACATCGATTCTCTAATTGATTTAAAGCAACACTTGTTCGATTTAATTGATGATGTTTTTTTTTTTTTTTTTGTGATAGTCGTCAAAGAGAAAGGTTAGGGAGCCAAAGGAAGAGACAGTAACATTGGGTCCAGCTGTTAAGGATGGTGAACATGTTTTCGGCGTTGCTCGCATTTTTGCTTCGTTTAACGACACCTTCATTGTTAGTATATATATGACTCTGTTTCTTTTTTTTTAGTTTGAATTATTGTTTTTTGAAGTTTGATTTTGATGATTTGAATGTTTTTTTTGTTACAGCATGTTACTGATTTATCCGGCAGGGAAACCCTCGTCCGTATCACTGGTATGTATGAGTTTTTTCTTCATATATCATTTTTCAGCTGTTTGTTAGTTATTAATTTTAGTTACTATCATATTTTAAGGTTTAATGTAGTTAAAAAATTACATATTTAAGGTATTTTTTTTTTTGAAAAACCTTAAAAATTTGGTTATTTGTATTAGACCAAGAACAATTATAGAGAGTTATAGCATGATTATTCTATACTCTGGTTTGTGTTAGGATTTTTATCTTTGTGGCGTTTGTTTAACTGACTCAAATTATTCCTAGGAACAACATACACTAGAATATAAAGACAGTAGTTTGAATCCAGGGTATTTTAAAAAAAATTCTTTGCCACACTCTTGAATATTCCTAGGAAAACTGGAATTTATTTTCTTTTAATAAAATTATACAAAATTTAATGGGAATGTTGGGTGTTGTGAGAAGTTATTTCATTCACAAGAACCTTTATACCCGGGAATAATTATTTTTCAATCCTGTAACAAATATGGGTATACTCAATTCCTAAGAGTGTTTTGACAAAAACTGTTTACGAGTGATTTGATAAGTTAATTTAGATGGAATTAATTTGATCAACACTAAGATAATATTATGTTATAAGTTTTTAATTTAGATGGAATTAATTTGATTAACCTAAGATACATTCATGTTGCTGTTGATATTGTTTGGTTTGGAATTGCATGTTTTAATTACTGGATGTGGTTTTCTCATATTTTTATTTTGTTTTTTTTCCAAATTTTAGGTGGGATGAAAGTTAAGGCTGACAGAGATGAGTCATCTCCCTATGCCGCTATGCTTGCAGCACAGGATGTTGCTACTAGATGCAAGGTCGGTGGATTTCTCATTAATATATAATGCTTTCTATTTACACTGATAACTTGTCAAACCAAAATGCGTTTTTGGGTGAAAATTGAACTGTGTTTACACTGTTAAATGGCTGTTTCTGAGCTTCAGTTTGAATGTTTTTGTTTTGTTTTGGCATTTGTTTAGTAAGTAGCATTATTTTTTTTTTATATGGGGAGCTGTCAAGTTTGTTGTACTGATTCAAAATATTTTGGAGCAGGAGCTCGGTATTACCGCACTTCACATTAAGCTCCGTGCCACTGGTGGAAACAAGACCAAAACACCTGGCCCTGGTGCTCAAGCTGCTCTTCGTGCCCTTGCTCGTTCTGGAATGAAAATTGGTCGTATAGGTATTTGCATTTACTGAAATTCTATTTTTTTGTTAACCCACAGAAGATAGTTTAAGGTTTACCTGCTCTCTGATATTTACGATGTGAAAGTTAGGAATGTTACTCTGTACGATCGATCATATAATACTAGACACCATCAGATTAAATACAATTATCGAAAAGAAAACTATATAGCCTCTATTTTACCAGAAGAGAGAGAATATACTTATTTCAAGTTATTTGAGTTATTTGGCGTGCGTATTGTATAGAATTATTTGGCGTGCGTATTGTATAGAATAACTGTAAGGTTGACTAATTGAGTCATGTAAGATATTCCAGCAGTTATAGTTAAGAGAAACATGGATATTAATATTCACTATAGTACATATGGTTTGTGTATGCTCTTATTTTAAGTTATTTGGCATCTGTACTGTATTGAACAACGGTCGAATTGCCGTAAATAAGGTTCACCGTTGATGAACTGAGTACGATATGGTAATAGGTAAAAGAGAATCAGGGATATCTAGGAAAATATGTTGCACAAGGAAATAATGATAAATCAAAGAAAAATAATGCACAAACCATAGAGATGAAGTGTCTATCCTTAGTCCCAAGACATTTGGATTTTCTGGTGGTGGTTGCTGTAGGTATTTTATATTTGCGTAATATTGTCCAGTCCAAAAAGAACTCACGTGGTAGATGCAACAAAATAATTTTGCATTCTTTCATTGCCTTCCCTGTTTGCTTGCTAAATCCTGTAATCACCGTTTCAGTCTAATCCACGAAAGGGTTGTCACATTTCCAGACTAGGAATTTTTTCAATTATAAAGATGATGTTTGTCTTTTTAAGTAAACCCTGACCTTATCTTCCTCTCAGTGAAATTAATTATTCTGTCAATCATCATTATTATTTCTACATTTTGTTGTATGTTATTTTCTAATTGATTTCATTTTTATGTTGCTATTGCAGAGGATGTCACTCCCATTCCTTCCGATAGCACCCGTAGAAAGAGTGGTAGAAGGGGTAGAAGGCTTTAG
Protein sequence

>MS.gene002746.t1

MSSKRKVREPKEETVTLGPAVKDGEHVFGVARIFASFNDTFIHVTDLSGRETLVRITGGMKVKADRDESSPYAAMLAAQDVATRCKELGITALHIKLRATGGNKTKTPGPGAQAALRALARSGMKIGRIEDVTPIPSDSTRRKSGRRGRRL