AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004435

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004435.t1 MTR_2g019670 99.338 151 1 0 1 151 1 151 1.12e-107 303
MS.gene004435.t1 MTR_4g096790 99.338 151 1 0 1 151 1 151 5.68e-107 301
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004435.t1 AT3G11510 89.855 138 14 0 3 140 2 139 8.87e-90 258
MS.gene004435.t1 AT3G52580 89.130 138 15 0 3 140 2 139 2.75e-89 256
MS.gene004435.t1 AT2G36160 88.406 138 16 0 3 140 2 139 1.64e-88 254
MS.gene004435.t1 ATCG00750 33.613 119 70 2 29 147 28 137 7.46e-12 59.7

Find 51 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCGTGCCCTTGCTCGTTC+TGG 0.240493 2.4:+67090241 MS.gene004435:CDS
AGCACCAGGGCCAGGTGTTT+TGG 0.248968 2.4:-67090209 None:intergenic
GCTCGTTCTGGAATGAAAAT+TGG 0.299156 2.4:+67090253 MS.gene004435:CDS
AATGTTACTGTCTCTTCCTT+TGG 0.327634 2.4:-67088853 None:intergenic
CCCTTCTACCACTCTTTCTA+CGG 0.354483 2.4:-67091264 None:intergenic
AGAGTGGTAGAAGGGGTAGA+AGG 0.383998 2.4:+67091272 MS.gene004435:CDS
AACATTGGGTCCAGCTGTTA+AGG 0.388167 2.4:+67088870 MS.gene004435:CDS
CGAAGAGCAGCTTGAGCACC+AGG 0.399712 2.4:-67090223 None:intergenic
AGTCGTCAAAGAGAAAGGTT+AGG 0.404664 2.4:+67088827 MS.gene004435:intron
TGCCGCTATGCTTGCAGCAC+AGG 0.424923 2.4:+67089877 MS.gene004435:CDS
TTTAGGTGGGATGAAAGTTA+AGG 0.429174 2.4:+67089829 MS.gene004435:intron
GGAATGAAAATTGGTCGTAT+AGG 0.435498 2.4:+67090262 MS.gene004435:CDS
GCAGCTTGAGCACCAGGGCC+AGG 0.435589 2.4:-67090217 None:intergenic
CAGCATGTTACTGATTTATC+CGG 0.442376 2.4:+67089044 MS.gene004435:intron
TGCTGCAAGCATAGCGGCAT+AGG 0.460137 2.4:-67089873 None:intergenic
GTGATAGTCGTCAAAGAGAA+AGG 0.460831 2.4:+67088822 MS.gene004435:intron
CACATTAAGCTCCGTGCCAC+TGG 0.465038 2.4:+67090175 MS.gene004435:CDS
CCCGTAGAAAGAGTGGTAGA+AGG 0.472164 2.4:+67091263 MS.gene004435:CDS
GAAACCCTTGTCCGTATCAC+TGG 0.485388 2.4:+67089071 MS.gene004435:CDS
TTTGGTCTTGTTTCCACCAG+TGG 0.501009 2.4:-67090191 None:intergenic
ACGGGTGCTATCGGAAGGAA+TGG 0.504362 2.4:-67091245 None:intergenic
ACGGACAAGGGTTTCCCTGC+CGG 0.518910 2.4:-67089063 None:intergenic
TGTTCACCATCCTTAACAGC+TGG 0.531081 2.4:-67088880 None:intergenic
ACTCTTTCTACGGGTGCTAT+CGG 0.532615 2.4:-67091254 None:intergenic
CCGTAGAAAGAGTGGTAGAA+GGG 0.533450 2.4:+67091264 MS.gene004435:CDS
AAAGGAAGAGACAGTAACAT+TGG 0.536923 2.4:+67088855 MS.gene004435:CDS
CCTTCTACCACTCTTTCTAC+GGG 0.539502 2.4:-67091263 None:intergenic
CATATATATACTAACAATGA+AGG 0.543037 2.4:-67088944 None:intergenic
ATGTTACTGATTTATCCGGC+AGG 0.543996 2.4:+67089048 MS.gene004435:CDS
GTCGTCAAAGAGAAAGGTTA+GGG 0.549356 2.4:+67088828 MS.gene004435:intron
AAGACCAAAACACCTGGCCC+TGG 0.552706 2.4:+67090205 MS.gene004435:CDS
AAGGAAGAGACAGTAACATT+GGG 0.554128 2.4:+67088856 MS.gene004435:CDS
TCTTGTTTCCACCAGTGGCA+CGG 0.555351 2.4:-67090186 None:intergenic
GAGAAAGGTTAGGGAGCCAA+AGG 0.560093 2.4:+67088837 MS.gene004435:CDS
ACTCATACATACCAGTGATA+CGG 0.570439 2.4:-67089082 None:intergenic
ATTAAGCTCCGTGCCACTGG+TGG 0.571382 2.4:+67090178 MS.gene004435:CDS
GCTGCAAGCATAGCGGCATA+GGG 0.578463 2.4:-67089872 None:intergenic
TTTCATTCCAGAACGAGCAA+GGG 0.582609 2.4:-67090248 None:intergenic
CGGGTGCTATCGGAAGGAAT+GGG 0.585535 2.4:-67091244 None:intergenic
GGAAACAAGACCAAAACACC+TGG 0.596535 2.4:+67090199 MS.gene004435:CDS
TTTCTACGGGTGCTATCGGA+AGG 0.607952 2.4:-67091250 None:intergenic
ACATACCAGTGATACGGACA+AGG 0.611084 2.4:-67089076 None:intergenic
TGTTACTGATTTATCCGGCA+GGG 0.618792 2.4:+67089049 MS.gene004435:CDS
TTGGGTCCAGCTGTTAAGGA+TGG 0.623308 2.4:+67088874 MS.gene004435:CDS
CGTAGAAAGAGTGGTAGAAG+GGG 0.630512 2.4:+67091265 MS.gene004435:CDS
GATAGCACCCGTAGAAAGAG+TGG 0.631829 2.4:+67091256 MS.gene004435:CDS
GGATGTTGCTACTAGATGCA+AGG 0.645225 2.4:+67089898 MS.gene004435:CDS
CATACCAGTGATACGGACAA+GGG 0.691082 2.4:-67089075 None:intergenic
ACGGAGCTTAATGTGAAGTG+CGG 0.698863 2.4:-67090167 None:intergenic
ATCCTGTGCTGCAAGCATAG+CGG 0.706099 2.4:-67089879 None:intergenic
GAAGAGCAGCTTGAGCACCA+GGG 0.706692 2.4:-67090222 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTAAAAAATTACATATTTA+AGG + chr2.4:67089182-67089201 MS.gene004435:intron 10.0%
!!! AGCATTATTTTTTTTTATAT+GGG + chr2.4:67090087-67090106 MS.gene004435:intron 10.0%
!!! TAGCATTATTTTTTTTTATA+TGG + chr2.4:67090086-67090105 MS.gene004435:intron 10.0%
!!! TTATAAGTTTTTAATTTAGA+TGG + chr2.4:67089686-67089705 MS.gene004435:intron 10.0%
!! ATTTGATAAGTTAATTTAGA+TGG + chr2.4:67089630-67089649 MS.gene004435:intron 15.0%
!! TTTGAAAAACCTTAAAAATT+TGG + chr2.4:67089216-67089235 MS.gene004435:intron 15.0%
!!! GCATTATTTTTTTTTATATG+GGG + chr2.4:67090088-67090107 MS.gene004435:intron 15.0%
!!! TACAAATAACCAAATTTTTA+AGG - chr2.4:67089228-67089247 None:intergenic 15.0%
!!! TTAGTTACTATCATATTTTA+AGG + chr2.4:67089150-67089169 MS.gene004435:intron 15.0%
!!! TTGTATTTTTTCCAAATTTT+AGG + chr2.4:67089812-67089831 MS.gene004435:intron 15.0%
!! AATATTCACTATAGTACATA+TGG + chr2.4:67090631-67090650 MS.gene004435:intron 20.0%
!! ATTGAAAAATAATTATTCCC+GGG - chr2.4:67089546-67089565 None:intergenic 20.0%
!! CATATATATACTAACAATGA+AGG - chr2.4:67088947-67088966 None:intergenic 20.0%
!! GATAATTGTATTTAATCTGA+TGG - chr2.4:67090420-67090439 None:intergenic 20.0%
!! GATTGAAAAATAATTATTCC+CGG - chr2.4:67089547-67089566 None:intergenic 20.0%
!! TATTGTATAGAATAACTGTA+AGG + chr2.4:67090548-67090567 MS.gene004435:intron 20.0%
!! TCAATCTTTGTAACAAATAT+GGG + chr2.4:67089561-67089580 MS.gene004435:intron 20.0%
!! TTCAATCTTTGTAACAAATA+TGG + chr2.4:67089560-67089579 MS.gene004435:intron 20.0%
!!! ATTTTTTCCAAATTTTAGGT+GGG + chr2.4:67089816-67089835 MS.gene004435:intron 20.0%
!!! GAATTTTTTTTAAAATACCC+TGG - chr2.4:67089400-67089419 None:intergenic 20.0%
!!! TATTTTTTCCAAATTTTAGG+TGG + chr2.4:67089815-67089834 MS.gene004435:intron 20.0%
!!! TGAATGTTTTTGTTTTGTTT+TGG + chr2.4:67090048-67090067 MS.gene004435:intron 20.0%
!!! TGCTCTTATTTTAAGTTATT+TGG + chr2.4:67090662-67090681 MS.gene004435:intron 20.0%
!!! TGTACTGATTCAAAATATTT+TGG + chr2.4:67090126-67090145 MS.gene004435:intron 20.0%
!!! TTCAAGTTATTTGAGTTATT+TGG + chr2.4:67090493-67090512 MS.gene004435:intron 20.0%
! AATTGAAAAAATTCCTAGTC+TGG - chr2.4:67091067-67091086 None:intergenic 25.0%
! ACAAAATTTAATGGGAATGT+TGG + chr2.4:67089481-67089500 MS.gene004435:intron 25.0%
! AGTTATAGTTAAGAGAAACA+TGG + chr2.4:67090603-67090622 MS.gene004435:intron 25.0%
! ATATTTACGATGTGAAAGTT+AGG + chr2.4:67090356-67090375 MS.gene004435:intron 25.0%
! CAAAATTTAATGGGAATGTT+GGG + chr2.4:67089482-67089501 MS.gene004435:intron 25.0%
! GAATAATTAATTTCACTGAG+AGG - chr2.4:67091135-67091154 None:intergenic 25.0%
! GCGTATTGTATAGAATTATT+TGG + chr2.4:67090519-67090538 MS.gene004435:intron 25.0%
! TAACTCTCTATAATTGTTCT+TGG - chr2.4:67089256-67089275 None:intergenic 25.0%
! TAATAGGTAAAAGAGAATCA+GGG + chr2.4:67090759-67090778 MS.gene004435:intron 25.0%
! TTTCTCTTAACTATAACTAC+TGG - chr2.4:67090601-67090620 None:intergenic 25.0%
!! AAATAAATTCCAGTTTTCCT+AGG - chr2.4:67089444-67089463 None:intergenic 25.0%
!! TGTTAGGATTTTTATCTTAG+TGG + chr2.4:67089304-67089323 MS.gene004435:intron 25.0%
!!! CATGTTTTAATTACTAGATG+TGG + chr2.4:67089771-67089790 MS.gene004435:intron 25.0%
AAACCTTAAACTATCTTCTG+TGG - chr2.4:67090324-67090343 None:intergenic 30.0%
AACCTTAAACTATCTTCTGT+GGG - chr2.4:67090323-67090342 None:intergenic 30.0%
ACTGTGTTTACACTGTTAAA+TGG + chr2.4:67090006-67090025 MS.gene004435:intron 30.0%
AGCATGATTATTCTATACTC+TGG + chr2.4:67089277-67089296 MS.gene004435:intron 30.0%
ATAATTATTCCCGGGTATAA+AGG - chr2.4:67089538-67089557 None:intergenic 30.0%
ATCTGTACTGTATTGAACAA+CGG + chr2.4:67090686-67090705 MS.gene004435:intron 30.0%
GTAATAGGTAAAAGAGAATC+AGG + chr2.4:67090758-67090777 MS.gene004435:intron 30.0%
TAAGTATATTCTCTCTCTTC+TGG - chr2.4:67090472-67090491 None:intergenic 30.0%
TTGAATATTCCTAGGAAAAC+TGG + chr2.4:67089432-67089451 MS.gene004435:intron 30.0%
! AACTGACTCAAATTATTCCT+AGG + chr2.4:67089336-67089355 MS.gene004435:intron 30.0%
! ATGTTGCTGTTGATATTGTT+TGG + chr2.4:67089738-67089757 MS.gene004435:intron 30.0%
! GTTTTTGTCAAAACACTCTT+AGG - chr2.4:67089598-67089617 None:intergenic 30.0%
! TCAAACCAAAATGCGTTTTT+GGG + chr2.4:67089973-67089992 MS.gene004435:intron 30.0%
! TGATGGTGTCTAGTATTATA+TGG - chr2.4:67090403-67090422 None:intergenic 30.0%
!! TTTTTATGTTGCTATTGCAG+AGG + chr2.4:67091213-67091232 MS.gene004435:intron 30.0%
!!! GATTCAAAATATTTTGGAGC+AGG + chr2.4:67090132-67090151 MS.gene004435:intron 30.0%
AAAGACAGTAGTTTGAATCC+AGG + chr2.4:67089379-67089398 MS.gene004435:intron 35.0%
AAAGAGAATCAGGGATATCT+AGG + chr2.4:67090768-67090787 MS.gene004435:intron 35.0%
AAAGGAAGAGACAGTAACAT+TGG + chr2.4:67088855-67088874 MS.gene004435:CDS 35.0%
AACCCACAGAAGATAGTTTA+AGG + chr2.4:67090318-67090337 MS.gene004435:intron 35.0%
AAGACAGTAGTTTGAATCCA+GGG + chr2.4:67089380-67089399 MS.gene004435:intron 35.0%
AAGGAAGAGACAGTAACATT+GGG + chr2.4:67088856-67088875 MS.gene004435:CDS 35.0%
AATGTTACTGTCTCTTCCTT+TGG - chr2.4:67088856-67088875 None:intergenic 35.0%
ACCAGAAAATCCAAATGTCT+TGG - chr2.4:67090876-67090895 None:intergenic 35.0%
ACTCATACATACCAGTGATA+CGG - chr2.4:67089085-67089104 None:intergenic 35.0%
ACTGAGTACGATATGGTAAT+AGG + chr2.4:67090743-67090762 MS.gene004435:intron 35.0%
ATTTCACTGAGAGGAAGATA+AGG - chr2.4:67091126-67091145 None:intergenic 35.0%
CAGCAACATGAATGTATCTT+AGG - chr2.4:67089727-67089746 None:intergenic 35.0%
CCAGAAAATCCAAATGTCTT+GGG - chr2.4:67090875-67090894 None:intergenic 35.0%
CTAGGAAAATATGTTGCACA+AGG + chr2.4:67090786-67090805 MS.gene004435:intron 35.0%
CTTTCATCCCACCTAAAATT+TGG - chr2.4:67089826-67089845 None:intergenic 35.0%
GCTGTTGATATTGTTTGGTT+TGG + chr2.4:67089743-67089762 MS.gene004435:intron 35.0%
GGAATGAAAATTGGTCGTAT+AGG + chr2.4:67090262-67090281 MS.gene004435:CDS 35.0%
TATCGTACTCAGTTCATCAA+CGG - chr2.4:67090736-67090755 None:intergenic 35.0%
TCTCTCTTCTGGTAAAATAG+AGG - chr2.4:67090461-67090480 None:intergenic 35.0%
TTCAAACTGAAGCTCAGAAA+CGG - chr2.4:67090032-67090051 None:intergenic 35.0%
TTCACAAGAACCTTTATACC+CGG + chr2.4:67089525-67089544 MS.gene004435:intron 35.0%
TTCTAGTGTATGTTGTTCCT+AGG - chr2.4:67089356-67089375 None:intergenic 35.0%
TTGATGAACTGAGTACGATA+TGG + chr2.4:67090736-67090755 MS.gene004435:intron 35.0%
TTTAGGTGGGATGAAAGTTA+AGG + chr2.4:67089829-67089848 MS.gene004435:intron 35.0%
TTTCAGTCTAATCCACGAAA+GGG + chr2.4:67091030-67091049 MS.gene004435:intron 35.0%
! AAGACATTTGGATTTTCTGG+TGG + chr2.4:67090875-67090894 MS.gene004435:intron 35.0%
! AAGGATGGTGAACATGTTTT+CGG + chr2.4:67088889-67088908 MS.gene004435:CDS 35.0%
! GTCAAACCAAAATGCGTTTT+TGG + chr2.4:67089972-67089991 MS.gene004435:intron 35.0%
! TCAACGGTGAACCTTATTTA+CGG - chr2.4:67090720-67090739 None:intergenic 35.0%
! TCTATACTCTGGTTTGTGTT+AGG + chr2.4:67089288-67089307 MS.gene004435:intron 35.0%
! TTTCACCCAAAAACGCATTT+TGG - chr2.4:67089981-67090000 None:intergenic 35.0%
!! CAGCATGTTACTGATTTATC+CGG + chr2.4:67089044-67089063 MS.gene004435:intron 35.0%
!! TTTCGTGGATTAGACTGAAA+CGG - chr2.4:67091030-67091049 None:intergenic 35.0%
ACAGGATTTAGCAAGCAAAC+AGG - chr2.4:67091002-67091021 None:intergenic 40.0%
AGTCGTCAAAGAGAAAGGTT+AGG + chr2.4:67088827-67088846 MS.gene004435:intron 40.0%
ATCCAAATGTCTTGGGACTA+AGG - chr2.4:67090868-67090887 None:intergenic 40.0%
ATCCTTAGTCCCAAGACATT+TGG + chr2.4:67090863-67090882 MS.gene004435:intron 40.0%
ATTTAGCAAGCAAACAGGGA+AGG - chr2.4:67090997-67091016 None:intergenic 40.0%
CAGGATTTAGCAAGCAAACA+GGG - chr2.4:67091001-67091020 None:intergenic 40.0%
CATCGTAAATATCAGAGAGC+AGG - chr2.4:67090348-67090367 None:intergenic 40.0%
CCACACTCTTGAATATTCCT+AGG + chr2.4:67089424-67089443 MS.gene004435:intron 40.0%
CCTAGGAATATTCAAGAGTG+TGG - chr2.4:67089427-67089446 None:intergenic 40.0%
GGATAGACACTTCATCTCTA+TGG - chr2.4:67090847-67090866 None:intergenic 40.0%
GTCGTCAAAGAGAAAGGTTA+GGG + chr2.4:67088828-67088847 MS.gene004435:intron 40.0%
GTGATAGTCGTCAAAGAGAA+AGG + chr2.4:67088822-67088841 MS.gene004435:intron 40.0%
GTTTCAGTCTAATCCACGAA+AGG + chr2.4:67091029-67091048 MS.gene004435:intron 40.0%
TAGACTGAAACGGTGATTAC+AGG - chr2.4:67091020-67091039 None:intergenic 40.0%
TCACAAGAACCTTTATACCC+GGG + chr2.4:67089526-67089545 MS.gene004435:intron 40.0%
TTTCATTCCAGAACGAGCAA+GGG - chr2.4:67090251-67090270 None:intergenic 40.0%
! CCCAAGACATTTGGATTTTC+TGG + chr2.4:67090872-67090891 MS.gene004435:intron 40.0%
! TCTACCACGTGAGTTCTTTT+TGG - chr2.4:67090943-67090962 None:intergenic 40.0%
! TTTTCATTCCAGAACGAGCA+AGG - chr2.4:67090252-67090271 None:intergenic 40.0%
!! ACATTTGGATTTTCTGGTGG+TGG + chr2.4:67090878-67090897 MS.gene004435:intron 40.0%
!! ATGTTACTGATTTATCCGGC+AGG + chr2.4:67089048-67089067 MS.gene004435:CDS 40.0%
!! GCTCGTTCTGGAATGAAAAT+TGG + chr2.4:67090253-67090272 MS.gene004435:CDS 40.0%
!! TGTTACTGATTTATCCGGCA+GGG + chr2.4:67089049-67089068 MS.gene004435:CDS 40.0%
!!! AATATTTTGGAGCAGGAGCT+CGG + chr2.4:67090139-67090158 MS.gene004435:intron 40.0%
AACATTGGGTCCAGCTGTTA+AGG + chr2.4:67088870-67088889 MS.gene004435:CDS 45.0%
ACATACCAGTGATACGGACA+AGG - chr2.4:67089079-67089098 None:intergenic 45.0%
ACGGAGCTTAATGTGAAGTG+CGG - chr2.4:67090170-67090189 None:intergenic 45.0%
ACTGAGAGGAAGATAAGGTC+AGG - chr2.4:67091121-67091140 None:intergenic 45.0%
CAGTCCAAAAAGAACTCACG+TGG + chr2.4:67090936-67090955 MS.gene004435:intron 45.0%
CATACCAGTGATACGGACAA+GGG - chr2.4:67089078-67089097 None:intergenic 45.0%
CCCTTCTACCACTCTTTCTA+CGG - chr2.4:67091267-67091286 None:intergenic 45.0%
CCGTAGAAAGAGTGGTAGAA+GGG + chr2.4:67091264-67091283 MS.gene004435:CDS 45.0%
CCTTCTACCACTCTTTCTAC+GGG - chr2.4:67091266-67091285 None:intergenic 45.0%
CGGTCGAATTGCCGTAAATA+AGG + chr2.4:67090706-67090725 MS.gene004435:intron 45.0%
CGTAGAAAGAGTGGTAGAAG+GGG + chr2.4:67091265-67091284 MS.gene004435:CDS 45.0%
CTGAGAGGAAGATAAGGTCA+GGG - chr2.4:67091120-67091139 None:intergenic 45.0%
GAAATGTGACAACCCTTTCG+TGG - chr2.4:67091045-67091064 None:intergenic 45.0%
GGAAACAAGACCAAAACACC+TGG + chr2.4:67090199-67090218 MS.gene004435:CDS 45.0%
GGATGTTGCTACTAGATGCA+AGG + chr2.4:67089898-67089917 MS.gene004435:CDS 45.0%
GGTTGTCACATTTCCAGACT+AGG + chr2.4:67091051-67091070 MS.gene004435:intron 45.0%
GTTGCTACTAGATGCAAGGT+CGG + chr2.4:67089902-67089921 MS.gene004435:intron 45.0%
TGTTCACCATCCTTAACAGC+TGG - chr2.4:67088883-67088902 None:intergenic 45.0%
! ACTCTTTCTACGGGTGCTAT+CGG - chr2.4:67091257-67091276 None:intergenic 45.0%
! CACGTGAGTTCTTTTTGGAC+TGG - chr2.4:67090938-67090957 None:intergenic 45.0%
!! TTTGGTCTTGTTTCCACCAG+TGG - chr2.4:67090194-67090213 None:intergenic 45.0%
!! AAAATTATACAAAATTTAAT+GGG + chr2.4:67089473-67089492 MS.gene004435:intron 5.0%
!! TAAAATTATACAAAATTTAA+TGG + chr2.4:67089472-67089491 MS.gene004435:intron 5.0%
AGAGTGGTAGAAGGGGTAGA+AGG + chr2.4:67091272-67091291 MS.gene004435:CDS 50.0%
ATCCTGTGCTGCAAGCATAG+CGG - chr2.4:67089882-67089901 None:intergenic 50.0%
CCCGTAGAAAGAGTGGTAGA+AGG + chr2.4:67091263-67091282 MS.gene004435:CDS 50.0%
GAAACCCTTGTCCGTATCAC+TGG + chr2.4:67089071-67089090 MS.gene004435:CDS 50.0%
GAGAAAGGTTAGGGAGCCAA+AGG + chr2.4:67088837-67088856 MS.gene004435:CDS 50.0%
GATAGCACCCGTAGAAAGAG+TGG + chr2.4:67091256-67091275 MS.gene004435:CDS 50.0%
TTGGGTCCAGCTGTTAAGGA+TGG + chr2.4:67088874-67088893 MS.gene004435:CDS 50.0%
! TCTTGTTTCCACCAGTGGCA+CGG - chr2.4:67090189-67090208 None:intergenic 50.0%
! TTTCTACGGGTGCTATCGGA+AGG - chr2.4:67091253-67091272 None:intergenic 50.0%
!! TTTCTGGTGGTGGTTGCTGT+AGG + chr2.4:67090888-67090907 MS.gene004435:intron 50.0%
AAGACCAAAACACCTGGCCC+TGG + chr2.4:67090205-67090224 MS.gene004435:CDS 55.0%
CACATTAAGCTCCGTGCCAC+TGG + chr2.4:67090175-67090194 MS.gene004435:CDS 55.0%
GAAGAGCAGCTTGAGCACCA+GGG - chr2.4:67090225-67090244 None:intergenic 55.0%
GCTGCAAGCATAGCGGCATA+GGG - chr2.4:67089875-67089894 None:intergenic 55.0%
TGCTGCAAGCATAGCGGCAT+AGG - chr2.4:67089876-67089895 None:intergenic 55.0%
! ACGGGTGCTATCGGAAGGAA+TGG - chr2.4:67091248-67091267 None:intergenic 55.0%
! ATTAAGCTCCGTGCCACTGG+TGG + chr2.4:67090178-67090197 MS.gene004435:CDS 55.0%
! CGGGTGCTATCGGAAGGAAT+GGG - chr2.4:67091247-67091266 None:intergenic 55.0%
ACGGACAAGGGTTTCCCTGC+CGG - chr2.4:67089066-67089085 None:intergenic 60.0%
CGAAGAGCAGCTTGAGCACC+AGG - chr2.4:67090226-67090245 None:intergenic 60.0%
CTTCGTGCCCTTGCTCGTTC+TGG + chr2.4:67090241-67090260 MS.gene004435:CDS 60.0%
TGCCGCTATGCTTGCAGCAC+AGG + chr2.4:67089877-67089896 MS.gene004435:CDS 60.0%
! AGCACCAGGGCCAGGTGTTT+TGG - chr2.4:67090212-67090231 None:intergenic 60.0%
GCAGCTTGAGCACCAGGGCC+AGG - chr2.4:67090220-67090239 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 67088724 67091300 67088724 ID=MS.gene004435
chr2.4 mRNA 67088724 67091300 67088724 ID=MS.gene004435.t1;Parent=MS.gene004435
chr2.4 exon 67088724 67088726 67088724 ID=MS.gene004435.t1.exon1;Parent=MS.gene004435.t1
chr2.4 CDS 67088724 67088726 67088724 ID=cds.MS.gene004435.t1;Parent=MS.gene004435.t1
chr2.4 exon 67088829 67088951 67088829 ID=MS.gene004435.t1.exon2;Parent=MS.gene004435.t1
chr2.4 CDS 67088829 67088951 67088829 ID=cds.MS.gene004435.t1;Parent=MS.gene004435.t1
chr2.4 exon 67089047 67089092 67089047 ID=MS.gene004435.t1.exon3;Parent=MS.gene004435.t1
chr2.4 CDS 67089047 67089092 67089047 ID=cds.MS.gene004435.t1;Parent=MS.gene004435.t1
chr2.4 exon 67089834 67089919 67089834 ID=MS.gene004435.t1.exon4;Parent=MS.gene004435.t1
chr2.4 CDS 67089834 67089919 67089834 ID=cds.MS.gene004435.t1;Parent=MS.gene004435.t1
chr2.4 exon 67090154 67090283 67090154 ID=MS.gene004435.t1.exon5;Parent=MS.gene004435.t1
chr2.4 CDS 67090154 67090283 67090154 ID=cds.MS.gene004435.t1;Parent=MS.gene004435.t1
chr2.4 exon 67091233 67091300 67091233 ID=MS.gene004435.t1.exon6;Parent=MS.gene004435.t1
chr2.4 CDS 67091233 67091300 67091233 ID=cds.MS.gene004435.t1;Parent=MS.gene004435.t1
Gene Sequence

>MS.gene004435

ATGGTATCTTCATACGTACATCGATTCTCTAATTGATTTAAAGCAACACTTGTTCGATTTAATTGATGATGTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGATAGTCGTCAAAGAGAAAGGTTAGGGAGCCAAAGGAAGAGACAGTAACATTGGGTCCAGCTGTTAAGGATGGTGAACATGTTTTCGGCGTTGCTCGCATTTTTGCTTCGTTTAACGACACCTTCATTGTTAGTATATATATGACTCTGTTTCTTTTTTTTTAGTTTGAATTATTGTTTTTTGAAGTTTGATTTTGATGATTTGAATGTTTTTTTTGTTACAGCATGTTACTGATTTATCCGGCAGGGAAACCCTTGTCCGTATCACTGGTATGTATGAGTTTTTTCTTCATATATCATTTTTCAGCTGTTTGTTAGTTATTAATTTTAGTTACTATCATATTTTAAGGTTTAATGTAGTTAAAAAATTACATATTTAAGGTATTTTTTTTTTTTGAAAAACCTTAAAAATTTGGTTATTTGTATTAGACCAAGAACAATTATAGAGAGTTATAGCATGATTATTCTATACTCTGGTTTGTGTTAGGATTTTTATCTTAGTGGCGTTTGTTTAACTGACTCAAATTATTCCTAGGAACAACATACACTAGAATATAAAGACAGTAGTTTGAATCCAGGGTATTTTAAAAAAAATTCTTTGCCACACTCTTGAATATTCCTAGGAAAACTGGAATTTATTTTCTTTTAATAAAATTATACAAAATTTAATGGGAATGTTGGGTGTTGTGAGAAGTTATTTCATTCACAAGAACCTTTATACCCGGGAATAATTATTTTTCAATCTTTGTAACAAATATGGGTATACTCAATTCCTAAGAGTGTTTTGACAAAAACTGTTTACGAGTGATTTGATAAGTTAATTTAGATGGAATTAATTTGATCAACACTAAGATAATATTATGTTATAAGTTTTTAATTTAGATGGAATTAATTTGATTAACCTAAGATACATTCATGTTGCTGTTGATATTGTTTGGTTTGGAATTGCATGTTTTAATTACTAGATGTGGTTTTCTCATATTTTTATTTTGTATTTTTTCCAAATTTTAGGTGGGATGAAAGTTAAGGCTGACAGAGATGAGTCATCTCCCTATGCCGCTATGCTTGCAGCACAGGATGTTGCTACTAGATGCAAGGTCGGTAGATTTCTCATTAATATATAATGCTTTCTATTTACACTGATAACTTGTCAAACCAAAATGCGTTTTTGGGTGAAAATTGAACTGTGTTTACACTGTTAAATGGCCGTTTCTGAGCTTCAGTTTGAATGTTTTTGTTTTGTTTTGGCATTTGTTTAGTAAGTAGCATTATTTTTTTTTATATGGGGAGCTGTCAAGTTTGTTGTACTGATTCAAAATATTTTGGAGCAGGAGCTCGGTATTACCGCACTTCACATTAAGCTCCGTGCCACTGGTGGAAACAAGACCAAAACACCTGGCCCTGGTGCTCAAGCTGCTCTTCGTGCCCTTGCTCGTTCTGGAATGAAAATTGGTCGTATAGGTATTTGCATTTACTGAAATTCTATTTTTTTGTTAACCCACAGAAGATAGTTTAAGGTTTACCTGCTCTCTGATATTTACGATGTGAAAGTTAGGAATGTTACTCTGTACGATCGACCATATAATACTAGACACCATCAGATTAAATACAATTATCGAAAAGAAAACTATATAGCCTCTATTTTACCAGAAGAGAGAGAATATACTTATTTCAAGTTATTTGAGTTATTTGGCGTGCGTATTGTATAGAATTATTTGGCGTGCGTATTGTATAGAATAACTGTAAGGTTGACTAATTGAGTCATGTAAGATATTCCAGTAGTTATAGTTAAGAGAAACATGGATATTAATATTCACTATAGTACATATGGTTTGTGTATGCTCTTATTTTAAGTTATTTGGCATCTGTACTGTATTGAACAACGGTCGAATTGCCGTAAATAAGGTTCACCGTTGATGAACTGAGTACGATATGGTAATAGGTAAAAGAGAATCAGGGATATCTAGGAAAATATGTTGCACAAGGAAATAATGATAAATCAAAGAAAAATAATGCACAAACCATAGAGATGAAGTGTCTATCCTTAGTCCCAAGACATTTGGATTTTCTGGTGGTGGTTGCTGTAGGTATTTTATATTTGCGTAATATTGTCCAGTCCAAAAAGAACTCACGTGGTAGATGCAACAAAATAATTTTGCATTCTTTCATTGCCTTCCCTGTTTGCTTGCTAAATCCTGTAATCACCGTTTCAGTCTAATCCACGAAAGGGTTGTCACATTTCCAGACTAGGAATTTTTTCAATTATAAAGATGATGTTTGTCTTTTTAAGTAAACCCTGACCTTATCTTCCTCTCAGTGAAATTAATTATTCTGTCAATCATCATTATTATTTCTACATTTTGTTGTATGTTATTTTCTAATTGATTTCATTTTTATGTTGCTATTGCAGAGGATGTCACTCCCATTCCTTCCGATAGCACCCGTAGAAAGAGTGGTAGAAGGGGTAGAAGGCTTTAG
Protein sequence

>MS.gene004435.t1

MSSKRKVREPKEETVTLGPAVKDGEHVFGVARIFASFNDTFIHVTDLSGRETLVRITGGMKVKADRDESSPYAAMLAAQDVATRCKELGITALHIKLRATGGNKTKTPGPGAQAALRALARSGMKIGRIEDVTPIPSDSTRRKSGRRGRRL