AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024612


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024612.t1 MTR_7g114410 97.727 176 4 0 1 176 1 176 2.02e-127 355
MS.gene024612.t1 MTR_7g114410 97.436 156 4 0 1 156 1 156 4.11e-112 316
MS.gene024612.t1 MTR_3g463610 62.573 171 61 1 4 171 3 173 1.21e-79 236
MS.gene024612.t1 MTR_7g024780 58.523 176 72 1 1 176 1 175 4.06e-71 213
MS.gene024612.t1 MTR_7g024765 60.920 174 66 2 1 174 1 172 4.52e-67 203
MS.gene024612.t1 MTR_7g024760 59.195 174 69 2 1 174 1 172 7.32e-67 202
MS.gene024612.t1 MTR_1g103150 46.784 171 86 2 4 171 19 187 6.47e-48 154
MS.gene024612.t1 MTR_1g103110 45.614 171 88 2 4 171 19 187 7.08e-47 152
MS.gene024612.t1 MTR_5g006450 42.442 172 96 1 3 171 16 187 1.47e-46 151
MS.gene024612.t1 MTR_5g006520 44.444 171 92 1 4 171 1735 1905 4.15e-45 159
MS.gene024612.t1 MTR_5g006470 43.787 169 94 1 4 171 17 185 2.12e-44 145
MS.gene024612.t1 MTR_5g006510 42.775 173 91 4 4 171 19 188 1.83e-41 138
MS.gene024612.t1 MTR_5g006460 43.429 175 90 5 4 171 18 190 1.86e-41 138
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024612.t1 AT3G22560 55.422 166 72 1 8 171 7 172 9.75e-64 194
MS.gene024612.t1 AT2G32030 47.305 167 84 2 8 173 24 187 4.77e-57 178
MS.gene024612.t1 AT2G32020 47.701 174 87 2 1 173 12 182 1.17e-56 177

Find 0 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAAAGTATACTTATATCAA+AGG - 51739:30933-30952 MS.gene024612:CDS 20.0%
!!! ATGATTTTTCTGATTTGTTA+AGG - 51739:30829-30848 MS.gene024612:CDS 20.0%
AGACATCTTTGATGAAAGTT+AGG + 51739:30626-30645 MS.gene024612:intergenic 30.0%
GACATCTTTGATGAAAGTTA+GGG + 51739:30625-30644 MS.gene024612:intergenic 30.0%
! TTTTACTTACGTGGATAACA+AGG - 51739:30860-30879 MS.gene024612:CDS 30.0%
!! TTATATCAAAGGTACCATCA+TGG - 51739:30944-30963 MS.gene024612:CDS 30.0%
!!! AGCAATTTTGATTGCTTTTG+TGG + 51739:30793-30812 MS.gene024612:intergenic 30.0%
!!! TTCTCAAAGAGTTTTGGAAA+AGG - 51739:30884-30903 MS.gene024612:CDS 30.0%
AAGTAACCAAAAACATCCGA+TGG - 51739:30574-30593 MS.gene024612:CDS 35.0%
ACGTTGACGATTTCATGTTA+TGG - 51739:30538-30557 MS.gene024612:CDS 35.0%
ATACACAGAAACAAAACCGA+TGG + 51739:30694-30713 MS.gene024612:intergenic 35.0%
ATGGAAAACTTGCAATTCGA+GGG - 51739:30593-30612 MS.gene024612:CDS 35.0%
CAGGAGACAAAATAATCAAC+AGG + 51739:31011-31030 MS.gene024612:intergenic 35.0%
CGTTGACGATTTCATGTTAT+GGG - 51739:30539-30558 MS.gene024612:CDS 35.0%
GAGACAAAATAATCAACAGG+AGG + 51739:31008-31027 MS.gene024612:intergenic 35.0%
! AGTTTTCCATCGGATGTTTT+TGG + 51739:30583-30602 MS.gene024612:intergenic 35.0%
! ATCTATCTAGGATATCTCTG+AGG - 51739:30499-30518 MS.gene024612:CDS 35.0%
! ATGCTGTTGCTTCTAACTAT+TGG - 51739:30754-30773 MS.gene024612:CDS 35.0%
! TCGAATTGCAAGTTTTCCAT+CGG + 51739:30593-30612 MS.gene024612:intergenic 35.0%
! TGCTGTTGCTTCTAACTATT+GGG - 51739:30755-30774 MS.gene024612:CDS 35.0%
!! TTCTGATTTGTTAAGGTTGC+AGG - 51739:30836-30855 MS.gene024612:CDS 35.0%
!!! AAAGAGTTTTGGAAAAGGCT+GGG - 51739:30889-30908 MS.gene024612:CDS 35.0%
!!! GTTTTGTTTCTGTGTATCCT+TGG - 51739:30697-30716 MS.gene024612:CDS 35.0%
AAAGACAGCCAAATCCATGA+TGG + 51739:30961-30980 MS.gene024611:intergenic 40.0%
ACGATTTCATGTTATGGGCA+GGG - 51739:30544-30563 MS.gene024612:CDS 40.0%
CAGAAACAAAACCGATGGAA+CGG + 51739:30689-30708 MS.gene024612:intergenic 40.0%
CTTCTAACTATTGGGGTCAA+GGG - 51739:30763-30782 MS.gene024612:CDS 40.0%
GATGGAAAACTTGCAATTCG+AGG - 51739:30592-30611 MS.gene024612:CDS 40.0%
! CAAGGCTTCTCAAAGAGTTT+TGG - 51739:30878-30897 MS.gene024612:CDS 40.0%
! GCTGTTGCTTCTAACTATTG+GGG - 51739:30756-30775 MS.gene024612:CDS 40.0%
! TCAACGTCTGTTAGCTTGAA+TGG + 51739:30525-30544 MS.gene024612:intergenic 40.0%
! TTCTAAGAACACCCTCTCTA+AGG + 51739:30917-30936 MS.gene024612:intergenic 40.0%
!! CAAAGGTACCATCATGGATT+TGG - 51739:30950-30969 MS.gene024612:CDS 40.0%
!!! CAAAGAGTTTTGGAAAAGGC+TGG - 51739:30888-30907 MS.gene024612:CDS 40.0%
ATCCTTGGTCAGGTGATGAT+AGG - 51739:30712-30731 MS.gene024612:CDS 45.0%
CGATTTCATGTTATGGGCAG+GGG - 51739:30545-30564 MS.gene024612:CDS 45.0%
GACGATTTCATGTTATGGGC+AGG - 51739:30543-30562 MS.gene024612:CDS 45.0%
GATAGGTGCAAAGCTGACAT+AGG - 51739:30729-30748 MS.gene024612:CDS 45.0%
GCTTCTAACTATTGGGGTCA+AGG - 51739:30762-30781 MS.gene024612:CDS 45.0%
GTTTCTGTGTATCCTTGGTC+AGG - 51739:30702-30721 MS.gene024612:CDS 45.0%
TGGCAAAGGTGGATCTATCT+AGG - 51739:30487-30506 MS.gene024612:CDS 45.0%
! GTTGCAGGCTTTTACTTACG+TGG - 51739:30851-30870 MS.gene024612:CDS 45.0%
AAAGGCTGGGTTCCTTAGAG+AGG - 51739:30902-30921 MS.gene024612:CDS 50.0%
AAGGCTGGGTTCCTTAGAGA+GGG - 51739:30903-30922 MS.gene024612:CDS 50.0%
AGACAGATCGAATGACGCCA+AGG + 51739:30660-30679 MS.gene024612:intergenic 50.0%
ATGTCTGCATACCTCACCCT+TGG - 51739:30640-30659 MS.gene024612:CDS 50.0%
CACCTATCATCACCTGACCA+AGG + 51739:30717-30736 MS.gene024612:intergenic 50.0%
GACAGATCGAATGACGCCAA+GGG + 51739:30659-30678 MS.gene024612:intergenic 50.0%
ATCGAATGACGCCAAGGGTG+AGG + 51739:30654-30673 MS.gene024612:intergenic 55.0%
TGTCTCGACGACCGTTCCAT+CGG - 51739:30675-30694 MS.gene024612:CDS 55.0%


Chromosome Type Strat End Strand Name
51739 gene 30486 31043 30486 ID=MS.gene024612
51739 mRNA 30486 31043 30486 ID=MS.gene024612.t1;Parent=MS.gene024612
51739 exon 30486 31043 30486 ID=MS.gene024612.t1.exon1;Parent=MS.gene024612.t1
51739 CDS 30486 31043 30486 ID=cds.MS.gene024612.t1;Parent=MS.gene024612.t1
Gene Sequence

>MS.gene024612

ATGGCAAAGGTGGATCTATCTAGGATATCTCTGAGGCCATTCAAGCTAACAGACGTTGACGATTTCATGTTATGGGCAGGGGACGATGAAGTAACCAAAAACATCCGATGGAAAACTTGCAATTCGAGGGAAGAAGCCCTAACTTTCATCAAAGATGTCTGCATACCTCACCCTTGGCGTCATTCGATCTGTCTCGACGACCGTTCCATCGGTTTTGTTTCTGTGTATCCTTGGTCAGGTGATGATAGGTGCAAAGCTGACATAGGTTATGCTGTTGCTTCTAACTATTGGGGTCAAGGGATAGCCACAAAAGCAATCAAAATTGCTCTGTCTCAAGTGTTTAATGATTTTTCTGATTTGTTAAGGTTGCAGGCTTTTACTTACGTGGATAACAAGGCTTCTCAAAGAGTTTTGGAAAAGGCTGGGTTCCTTAGAGAGGGTGTTCTTAGAAAGTATACTTATATCAAAGGTACCATCATGGATTTGGCTGTCTTTAGTTTTTTGTCAACAGATGAAATTCCTCCTGTTGATTATTTTGTCTCCTGTTACTCTTTATGA

Protein sequence

>MS.gene024612.t1

MAKVDLSRISLRPFKLTDVDDFMLWAGDDEVTKNIRWKTCNSREEALTFIKDVCIPHPWRHSICLDDRSIGFVSVYPWSGDDRCKADIGYAVASNYWGQGIATKAIKIALSQVFNDFSDLLRLQAFTYVDNKASQRVLEKAGFLREGVLRKYTYIKGTIMDLAVFSFLSTDEIPPVDYFVSCYSL