AlfalfaGEDB | MS.gene21833

M. sativa cultivar XinJiangDaYe / MS.gene21833

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21833.t1	XP_003610737.1	92.6	190	14	0	1	190	1	190	1.10E-92	349.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21833.t1	P05332	31.8	179	111	3	15	187	2	175	8.6e-14	78.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21833.t1	G7K3P2	92.6	190	14	0	1	190	1	190	7.8e-93	349.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene21833.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene21833	MS.gene050493	PPI
MS.gene21833	MS.gene070945	PPI
MS.gene29937	MS.gene21833	PPI
MS.gene21833	MS.gene001033	PPI
MS.gene21833	MS.gene024854	PPI
MS.gene006418	MS.gene21833	PPI
MS.gene21833	MS.gene22888	PPI
MS.gene21833	MS.gene04530	PPI
MS.gene21833	MS.gene043813	PPI
MS.gene21833	MS.gene024614	PPI
MS.gene21833	MS.gene21831	PPI
MS.gene21833	MS.gene024612	PPI
MS.gene024854	MS.gene21833	PPI
MS.gene21833	MS.gene21830	PPI
MS.gene21833	MS.gene34371	PPI
MS.gene21833	MS.gene001034	PPI
MS.gene21833	MS.gene21827	PPI
MS.gene21833	MS.gene070944	PPI
MS.gene001033	MS.gene21833	PPI
MS.gene21833	MS.gene071361	PPI
MS.gene49468	MS.gene21833	PPI
MS.gene34370	MS.gene21833	PPI
MS.gene21833	MS.gene49470	PPI
MS.gene21833	MS.gene77154	PPI
MS.gene21833	MS.gene49468	PPI
MS.gene21833	MS.gene006417	PPI
MS.gene77154	MS.gene21833	PPI
MS.gene21833	MS.gene74082	PPI
MS.gene21833	MS.gene024857	PPI
MS.gene21833	MS.gene95801	PPI
MS.gene21833	MS.gene001037	PPI
MS.gene043815	MS.gene21833	PPI
MS.gene95802	MS.gene21833	PPI
MS.gene21833	MS.gene071357	PPI
MS.gene21833	MS.gene21828	PPI
MS.gene49472	MS.gene21833	PPI
MS.gene024858	MS.gene21833	PPI
MS.gene21833	MS.gene024859	PPI
MS.gene21833	MS.gene95802	PPI
MS.gene024855	MS.gene21833	PPI
MS.gene21833	MS.gene001032	PPI
MS.gene21833	MS.gene49471	PPI
MS.gene21833	MS.gene006418	PPI
MS.gene001038	MS.gene21833	PPI
MS.gene21830	MS.gene21833	PPI
MS.gene21833	MS.gene001038	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21833.t1	MTR_5g006450	92.632	190	14	0	1	190	1	190	3.01e-127	356
MS.gene21833.t1	MTR_5g006470	76.316	190	43	1	1	190	1	188	1.19e-101	291
MS.gene21833.t1	MTR_5g006520	76.842	190	44	0	1	190	1719	1908	2.33e-96	306
MS.gene21833.t1	MTR_5g006520	50.667	75	14	1	97	171	1655	1706	1.09e-11	63.2
MS.gene21833.t1	MTR_5g006460	74.359	195	43	5	1	190	1	193	1.37e-95	276
MS.gene21833.t1	MTR_5g006510	73.298	191	44	3	1	190	7	191	1.38e-94	273
MS.gene21833.t1	MTR_1g103110	63.542	192	66	2	1	190	1	190	7.97e-84	246
MS.gene21833.t1	MTR_1g103150	59.375	192	74	2	1	190	1	190	4.79e-78	231
MS.gene21833.t1	MTR_7g114410	41.860	172	97	1	16	187	3	171	8.18e-47	152
MS.gene21833.t1	MTR_3g463610	39.766	171	103	0	17	187	3	173	3.99e-44	146
MS.gene21833.t1	MTR_7g114410	41.401	157	89	1	16	172	3	156	8.94e-43	140
MS.gene21833.t1	MTR_7g024760	41.667	168	93	3	20	187	7	169	3.99e-38	129
MS.gene21833.t1	MTR_7g024780	39.766	171	99	2	17	187	4	170	9.09e-38	128
MS.gene21833.t1	MTR_7g024765	39.286	168	97	3	20	187	7	169	3.02e-36	124
MS.gene21833.t1	MTR_1g103320	53.846	78	34	1	53	130	13	88	1.15e-19	80.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21833.t1	AT2G32030	51.190	168	75	3	21	187	24	185	4.17e-57	178
MS.gene21833.t1	AT2G32020	50.000	168	77	2	21	187	19	180	1.03e-52	167
MS.gene21833.t1	AT3G22560	38.235	170	100	2	20	187	6	172	3.16e-36	124

Find 50 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAAATCAATTCTCTCTTCTT+TGG	0.250774	5.2:+1213886	None:intergenic
ATGTTCTAGGCTCTAAATAT+TGG	0.292142	5.2:-1213604	MS.gene21833:CDS
TCCTTAGAACTCCCTCCTTT+TGG	0.297796	5.2:+1213444	None:intergenic
GCAGAACTTGGCTATGTTCT+AGG	0.302641	5.2:-1213617	MS.gene21833:CDS
TGTTCTAGGCTCTAAATATT+GGG	0.316974	5.2:-1213603	MS.gene21833:CDS
GAAGGGCTCGGAGTGTGATT+TGG	0.319800	5.2:+1213861	None:intergenic
GAGTTCCCAAGAGCAGAATT+TGG	0.329740	5.2:+1213787	None:intergenic
GCTCTAAATATTGGGGTAAA+GGG	0.356917	5.2:-1213595	MS.gene21833:CDS
AAGGGCTCGGAGTGTGATTT+GGG	0.367793	5.2:+1213862	None:intergenic
CTAAACAATTGAATTATAGT+TGG	0.392086	5.2:+1213357	None:intergenic
TGCATCAATGATCATGCAAT+TGG	0.393506	5.2:-1213692	MS.gene21833:CDS
ATATAGCTACCAAGTTTCTA+TGG	0.402105	5.2:-1213727	MS.gene21833:CDS
TCCAAAAGGAGGGAGTTCTA+AGG	0.410439	5.2:-1213445	MS.gene21833:CDS
GTTCACCTGGTGATAAATCT+AGG	0.421080	5.2:-1213649	MS.gene21833:CDS
GGCTCTAAATATTGGGGTAA+AGG	0.422886	5.2:-1213596	MS.gene21833:CDS
AAGTGGCCAAATTCTGCTCT+TGG	0.423997	5.2:-1213793	MS.gene21833:CDS
GTTGATGCCATCATCCTTGC+TGG	0.429573	5.2:+1213760	None:intergenic
GTGAGTTGTCTTACTTGAAA+AGG	0.431397	5.2:-1213529	MS.gene21833:CDS
TGCTGGGTCTCAAAGAGTGC+TGG	0.460731	5.2:-1213480	MS.gene21833:CDS
AGTGGCCAAATTCTGCTCTT+GGG	0.474265	5.2:-1213792	MS.gene21833:CDS
AGGAACAAATGTGCAGAACT+TGG	0.477895	5.2:-1213629	MS.gene21833:CDS
AGGAAATATTTGTTCATGAA+AGG	0.480337	5.2:-1213425	MS.gene21833:CDS
GCTTGAAGCTTTAGTTGATG+TGG	0.488968	5.2:-1213507	MS.gene21833:CDS
TGCTTTGCACCATAGAAACT+TGG	0.496119	5.2:+1213718	None:intergenic
ATTCTCTCTTCTTTGGCACA+TGG	0.499559	5.2:+1213893	None:intergenic
TTCTGACCTTGATGATCTCA+TGG	0.505164	5.2:-1213834	MS.gene21833:CDS
CATCAAGGTCAGAAAGTTGA+AGG	0.505953	5.2:+1213843	None:intergenic
TGTTGTGAAACAAGTGGTTA+AGG	0.511157	5.2:-1213561	MS.gene21833:CDS
TTAGTTGATGTGGAAAATGC+TGG	0.527178	5.2:-1213497	MS.gene21833:CDS
GGTCAGAAAGTTGAAGGGCT+CGG	0.528148	5.2:+1213849	None:intergenic
TCTCAAAGAGTGCTGGAGAA+AGG	0.534460	5.2:-1213473	MS.gene21833:CDS
TTGTTCCTAGATTTATCACC+AGG	0.541277	5.2:+1213644	None:intergenic
TCTCTATCCTCAAGTTCACC+TGG	0.545696	5.2:-1213662	MS.gene21833:CDS
CTCTACACCAGCAAGGATGA+TGG	0.559987	5.2:-1213767	MS.gene21833:CDS
GAAAGGTGGTTTCCAAAAGG+AGG	0.562242	5.2:-1213456	MS.gene21833:CDS
CTCTAAATATTGGGGTAAAG+GGG	0.564900	5.2:-1213594	MS.gene21833:CDS
GGAGAAAGGTGGTTTCCAAA+AGG	0.565921	5.2:-1213459	MS.gene21833:CDS
TAGTTGATGTGGAAAATGCT+GGG	0.573363	5.2:-1213496	MS.gene21833:CDS
TTTATCACCAGGTGAACTTG+AGG	0.575731	5.2:+1213655	None:intergenic
GCATCAATGATCATGCAATT+GGG	0.576565	5.2:-1213691	MS.gene21833:CDS
CACTTGTTTCACAACACATG+TGG	0.594420	5.2:+1213568	None:intergenic
ACCTTGATGATCTCATGGTG+TGG	0.606125	5.2:-1213829	MS.gene21833:CDS
GTTCTAGGCTCTAAATATTG+GGG	0.619040	5.2:-1213602	MS.gene21833:CDS
CAAAGAGTGCTGGAGAAAGG+TGG	0.629282	5.2:-1213470	MS.gene21833:CDS
TCCACACCATGAGATCATCA+AGG	0.646997	5.2:+1213828	None:intergenic
AAAGGTGGTTTCCAAAAGGA+GGG	0.651209	5.2:-1213455	MS.gene21833:CDS
ATCAAGGTCAGAAAGTTGAA+GGG	0.653293	5.2:+1213844	None:intergenic
GAGATCAGTAGAAAGAACAC+TGG	0.658551	5.2:+1213379	None:intergenic
TTGGGAACTCTACACCAGCA+AGG	0.679749	5.2:-1213774	MS.gene21833:CDS
CACATGTGTTGTGAAACAAG+TGG	0.736637	5.2:-1213567	MS.gene21833:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGGAAATATTTGTTCATGAA+AGG	-	chr5.2:1213865-1213884	MS.gene21833:CDS	25.0%
!	TAAATCAATTCTCTCTTCTT+TGG	+	chr5.2:1213407-1213426	None:intergenic	25.0%
	ATATAGCTACCAAGTTTCTA+TGG	-	chr5.2:1213563-1213582	MS.gene21833:CDS	30.0%
!!	ATGTTCTAGGCTCTAAATAT+TGG	-	chr5.2:1213686-1213705	MS.gene21833:CDS	30.0%
!!	TGTTCTAGGCTCTAAATATT+GGG	-	chr5.2:1213687-1213706	MS.gene21833:CDS	30.0%
	ATCAAGGTCAGAAAGTTGAA+GGG	+	chr5.2:1213449-1213468	None:intergenic	35.0%
	CTCTAAATATTGGGGTAAAG+GGG	-	chr5.2:1213696-1213715	MS.gene21833:CDS	35.0%
	GCATCAATGATCATGCAATT+GGG	-	chr5.2:1213599-1213618	MS.gene21833:CDS	35.0%
	GTGAGTTGTCTTACTTGAAA+AGG	-	chr5.2:1213761-1213780	MS.gene21833:CDS	35.0%
	TGCATCAATGATCATGCAAT+TGG	-	chr5.2:1213598-1213617	MS.gene21833:CDS	35.0%
	TGTTGTGAAACAAGTGGTTA+AGG	-	chr5.2:1213729-1213748	MS.gene21833:CDS	35.0%
	TTGTTCCTAGATTTATCACC+AGG	+	chr5.2:1213649-1213668	None:intergenic	35.0%
!	GCTCTAAATATTGGGGTAAA+GGG	-	chr5.2:1213695-1213714	MS.gene21833:CDS	35.0%
!	TAGTTGATGTGGAAAATGCT+GGG	-	chr5.2:1213794-1213813	MS.gene21833:CDS	35.0%
!	TTAGTTGATGTGGAAAATGC+TGG	-	chr5.2:1213793-1213812	MS.gene21833:CDS	35.0%
!!	GTTCTAGGCTCTAAATATTG+GGG	-	chr5.2:1213688-1213707	MS.gene21833:CDS	35.0%
	AAAGGTGGTTTCCAAAAGGA+GGG	-	chr5.2:1213835-1213854	MS.gene21833:CDS	40.0%
	AGGAACAAATGTGCAGAACT+TGG	-	chr5.2:1213661-1213680	MS.gene21833:CDS	40.0%
	ATTCTCTCTTCTTTGGCACA+TGG	+	chr5.2:1213400-1213419	None:intergenic	40.0%
	CACATGTGTTGTGAAACAAG+TGG	-	chr5.2:1213723-1213742	MS.gene21833:CDS	40.0%
	CACTTGTTTCACAACACATG+TGG	+	chr5.2:1213725-1213744	None:intergenic	40.0%
	CATCAAGGTCAGAAAGTTGA+AGG	+	chr5.2:1213450-1213469	None:intergenic	40.0%
	GAGATCAGTAGAAAGAACAC+TGG	+	chr5.2:1213914-1213933	None:intergenic	40.0%
	GTGGACAACTGATGAAAAAG+TGG	-	chr5.2:1213480-1213499	MS.gene21833:CDS	40.0%
	GTTCACCTGGTGATAAATCT+AGG	-	chr5.2:1213641-1213660	MS.gene21833:CDS	40.0%
	TGCTTTGCACCATAGAAACT+TGG	+	chr5.2:1213575-1213594	None:intergenic	40.0%
	TTTATCACCAGGTGAACTTG+AGG	+	chr5.2:1213638-1213657	None:intergenic	40.0%
!	GCTTGAAGCTTTAGTTGATG+TGG	-	chr5.2:1213783-1213802	MS.gene21833:CDS	40.0%
!	TTCTGACCTTGATGATCTCA+TGG	-	chr5.2:1213456-1213475	MS.gene21833:CDS	40.0%
!!	GGCTCTAAATATTGGGGTAA+AGG	-	chr5.2:1213694-1213713	MS.gene21833:CDS	40.0%
	AAGTGGCCAAATTCTGCTCT+TGG	-	chr5.2:1213497-1213516	MS.gene21833:CDS	45.0%
	AGTGGCCAAATTCTGCTCTT+GGG	-	chr5.2:1213498-1213517	MS.gene21833:CDS	45.0%
	GAAAGGTGGTTTCCAAAAGG+AGG	-	chr5.2:1213834-1213853	MS.gene21833:CDS	45.0%
	GAGTTCCCAAGAGCAGAATT+TGG	+	chr5.2:1213506-1213525	None:intergenic	45.0%
	GCAGAACTTGGCTATGTTCT+AGG	-	chr5.2:1213673-1213692	MS.gene21833:CDS	45.0%
	GGAGAAAGGTGGTTTCCAAA+AGG	-	chr5.2:1213831-1213850	MS.gene21833:CDS	45.0%
	TCCAAAAGGAGGGAGTTCTA+AGG	-	chr5.2:1213845-1213864	MS.gene21833:CDS	45.0%
	TCCACACCATGAGATCATCA+AGG	+	chr5.2:1213465-1213484	None:intergenic	45.0%
	TCTCTATCCTCAAGTTCACC+TGG	-	chr5.2:1213628-1213647	MS.gene21833:CDS	45.0%
!	ACCTTGATGATCTCATGGTG+TGG	-	chr5.2:1213461-1213480	MS.gene21833:CDS	45.0%
!	TCCTTAGAACTCCCTCCTTT+TGG	+	chr5.2:1213849-1213868	None:intergenic	45.0%
!!	TCTCAAAGAGTGCTGGAGAA+AGG	-	chr5.2:1213817-1213836	MS.gene21833:CDS	45.0%
	AAGGGCTCGGAGTGTGATTT+GGG	+	chr5.2:1213431-1213450	None:intergenic	50.0%
	CTCTACACCAGCAAGGATGA+TGG	-	chr5.2:1213523-1213542	MS.gene21833:CDS	50.0%
	GGTCAGAAAGTTGAAGGGCT+CGG	+	chr5.2:1213444-1213463	None:intergenic	50.0%
	GTTGATGCCATCATCCTTGC+TGG	+	chr5.2:1213533-1213552	None:intergenic	50.0%
	TTGGGAACTCTACACCAGCA+AGG	-	chr5.2:1213516-1213535	MS.gene21833:CDS	50.0%
!!	CAAAGAGTGCTGGAGAAAGG+TGG	-	chr5.2:1213820-1213839	MS.gene21833:CDS	50.0%
	GAAGGGCTCGGAGTGTGATT+TGG	+	chr5.2:1213432-1213451	None:intergenic	55.0%
!	TGCTGGGTCTCAAAGAGTGC+TGG	-	chr5.2:1213810-1213829	MS.gene21833:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	1213370	1213942	1213370	ID=MS.gene21833
chr5.2	mRNA	1213370	1213942	1213370	ID=MS.gene21833.t1;Parent=MS.gene21833
chr5.2	exon	1213370	1213942	1213370	ID=MS.gene21833.t1.exon1;Parent=MS.gene21833.t1
chr5.2	CDS	1213370	1213942	1213370	ID=cds.MS.gene21833.t1;Parent=MS.gene21833.t1

Gene Sequence

>MS.gene21833

ATGGAAAGAACTTCTATTTCTTTCGAACCATGTGCCAAAGAAGAGAGAATTGATTTAACCCAAATCACACTCCGAGCCCTTCAACTTTCTGACCTTGATGATCTCATGGTGTGGACAACTGATGAAAAAGTGGCCAAATTCTGCTCTTGGGAACTCTACACCAGCAAGGATGATGGCATCAACTTCATTGAAAATATAGCTACCAAGTTTCTATGGTGCAAAGCAATATGCATCAATGATCATGCAATTGGGTGTGTTTCTCTATCCTCAAGTTCACCTGGTGATAAATCTAGGAACAAATGTGCAGAACTTGGCTATGTTCTAGGCTCTAAATATTGGGGTAAAGGGGTTGCCACATGTGTTGTGAAACAAGTGGTTAAGGTTGCATTCTGTGAGTTGTCTTACTTGAAAAGGCTTGAAGCTTTAGTTGATGTGGAAAATGCTGGGTCTCAAAGAGTGCTGGAGAAAGGTGGTTTCCAAAAGGAGGGAGTTCTAAGGAAATATTTGTTCATGAAAGGAAAAAGTAGAGATATGATCATGTCCAGTGTTCTTTCTACTGATCTCCAACTATAA

Protein sequence

>MS.gene21833.t1

MERTSISFEPCAKEERIDLTQITLRALQLSDLDDLMVWTTDEKVAKFCSWELYTSKDDGINFIENIATKFLWCKAICINDHAIGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVATCVVKQVVKVAFCELSYLKRLEALVDVENAGSQRVLEKGGFQKEGVLRKYLFMKGKSRDMIMSSVLSTDLQL