AlfalfaGEDB | MS.gene77154

M. sativa cultivar XinJiangDaYe / MS.gene77154

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77154.t1	XP_003610737.1	85.1	174	25	1	1	173	1	174	2.40E-75	291.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77154.t1	G7K3P2	85.1	174	25	1	1	173	1	174	1.8e-75	291.6

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene77154.t1	TR	GNAT

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene001033	MS.gene77154	PPI
MS.gene001038	MS.gene77154	PPI
MS.gene006418	MS.gene77154	PPI
MS.gene024854	MS.gene77154	PPI
MS.gene024855	MS.gene77154	PPI
MS.gene024858	MS.gene77154	PPI
MS.gene043815	MS.gene77154	PPI
MS.gene21830	MS.gene77154	PPI
MS.gene21833	MS.gene77154	PPI
MS.gene29937	MS.gene77154	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77154.t1	MTR_5g006450	85.057	174	25	1	1	173	1	174	2.35e-103	295
MS.gene77154.t1	MTR_5g006470	69.540	174	50	2	1	173	1	172	2.01e-76	226
MS.gene77154.t1	MTR_5g006520	72.561	164	44	1	1	163	1719	1882	1.83e-74	243
MS.gene77154.t1	MTR_5g006510	68.293	164	44	4	1	162	7	164	7.79e-68	205
MS.gene77154.t1	MTR_5g006460	68.452	168	45	6	1	162	1	166	5.23e-66	200
MS.gene77154.t1	MTR_1g103110	59.394	165	62	3	1	162	1	163	1.12e-60	187
MS.gene77154.t1	MTR_1g103150	56.970	165	66	3	1	162	1	163	1.39e-57	179
MS.gene77154.t1	MTR_7g114410	35.294	136	85	1	16	151	3	135	3.51e-27	100
MS.gene77154.t1	MTR_7g114410	35.294	136	85	1	16	151	3	135	7.46e-27	100
MS.gene77154.t1	MTR_3g463610	33.813	139	92	0	17	155	3	141	1.55e-26	100

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77154.t1	AT2G32030	46.104	154	68	4	3	155	14	153	8.11e-40	134
MS.gene77154.t1	AT2G32020	47.794	136	64	2	21	155	19	148	3.38e-36	124
MS.gene77154.t1	AT3G22560	33.793	145	90	3	21	162	7	148	1.87e-20	83.6

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Find 43 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGGGTCGGAGTGTGATTT+GGG	0.320072	2.4:+32047858	None:intergenic
AAGTTGCCAAGTTCTGCTCT+TGG	0.477212	2.4:-32047789	MS.gene77154:CDS
AATGTTGGGTCTCAGAGTTT+TGG	0.354229	2.4:-32047478	MS.gene77154:CDS
ACCTTGATGATCTCATGGTA+TGG	0.538119	2.4:-32047825	MS.gene77154:CDS
AGGAAATATTTGTTCATGAA+AGG	0.480337	2.4:-32047423	MS.gene77154:CDS
AGGAACGAATGTGCAGAACT+TGG	0.523520	2.4:-32047625	MS.gene77154:CDS
AGTTGCCAAGTTCTGCTCTT+GGG	0.428431	2.4:-32047788	MS.gene77154:CDS
ATATAGCTTCAAAGTTTCTA+TGG	0.332117	2.4:-32047723	MS.gene77154:CDS
ATCAAGGTCAGAAAGATTAA+GGG	0.492658	2.4:+32047840	None:intergenic
ATGTTCTAGGCTCTAAATAT+TGG	0.292142	2.4:-32047600	MS.gene77154:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCAAGGTCAGAAAGATTAA+GGG	+	chr2.4:32047493-32047512	None:intergenic	30.0%
ATTCTGTGAGTTGTCTTACT+TGG	-	chr2.4:32047800-32047819	MS.gene77154:CDS	35.0%
CATCAAGGTCAGAAAGATTA+AGG	+	chr2.4:32047494-32047513	None:intergenic	35.0%
CTCTAAATATTGGGGTAAAG+GGG	-	chr2.4:32047740-32047759	MS.gene77154:CDS	35.0%
TCAAGGTCAGAAAGATTAAG+GGG	+	chr2.4:32047492-32047511	None:intergenic	35.0%
TGTTGTGAAACAAGTGGTTA+AGG	-	chr2.4:32047773-32047792	MS.gene77154:CDS	35.0%
ATTCTCTCTTCTTTGTCACG+TGG	+	chr2.4:32047444-32047463	None:intergenic	40.0%
CAAGTGTGTTGTGAAACAAG+TGG	-	chr2.4:32047767-32047786	MS.gene77154:CDS	40.0%
CACTTGTTTCACAACACACT+TGG	+	chr2.4:32047769-32047788	None:intergenic	40.0%
CCATCAATGATCGTGCAATT+GGG	-	chr2.4:32047643-32047662	MS.gene77154:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	32047414	32047938	32047414	ID=MS.gene77154
chr2.4	mRNA	32047414	32047938	32047414	ID=MS.gene77154.t1;Parent=MS.gene77154
chr2.4	exon	32047414	32047938	32047414	ID=MS.gene77154.t1.exon1;Parent=MS.gene77154.t1
chr2.4	CDS	32047414	32047938	32047414	ID=cds.MS.gene77154.t1;Parent=MS.gene77154.t1

Gene Sequence

>MS.gene77154

ATGGAGATAACTTCTATTTCTTCCAAACCACGTGACAAAGAAGAGAGAATTGATCTAACCCAAATCACACTCCGACCCCTTAATCTTTCTGACCTTGATGATCTCATGGTATGGACAACTGATGAAAAAGTTGCCAAGTTCTGCTCTTGGGAACTCTACACCAGCAAGGATGATGGCATCAACTTCATTGAAAATATAGCTTCAAAGTTTCTATGGTGCAAAGCAATATCCATCAATGATCGTGCAATTGGGTGTGTTTCTCTATCCTCAAGTTCACCTGGTGATAAATCAAGGAACGAATGTGCAGAACTTGGCTATGTTCTAGGCTCTAAATATTGGGGTAAAGGGGTTGCCAAGTGTGTTGTGAAACAAGTGGTTAAGGCTGCATTCTGTGAGTTGTCTTACTTGGAAAGGCTTGAAGCTTTAGTTGATGTGGAAAATGTTGGGTCTCAGAGTTTTGGAGAAAGCTGGTTTCCAAAAAGAGGGAGTTCTAAGGAAATATTTGTTCATGAAAGGAAAAAGTAG

Protein sequence

>MS.gene77154.t1

MEITSISSKPRDKEERIDLTQITLRPLNLSDLDDLMVWTTDEKVAKFCSWELYTSKDDGINFIENIASKFLWCKAISINDRAIGCVSLSSSSPGDKSRNECAELGYVLGSKYWGKGVAKCVVKQVVKAAFCELSYLERLEALVDVENVGSQSFGESWFPKRGSSKEIFVHERKK