AlfalfaGEDB | MS.gene77154

M. sativa cultivar XinJiangDaYe / MS.gene77154

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77154.t1	XP_003610737.1	85.1	174	25	1	1	173	1	174	2.40E-75	291.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77154.t1	G7K3P2	85.1	174	25	1	1	173	1	174	1.8e-75	291.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene77154.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene77154	MS.gene043813	PPI
MS.gene77154	MS.gene024614	PPI
MS.gene77154	MS.gene21831	PPI
MS.gene77154	MS.gene024612	PPI
MS.gene77154	MS.gene21830	PPI
MS.gene77154	MS.gene34371	PPI
MS.gene77154	MS.gene001034	PPI
MS.gene77154	MS.gene21827	PPI
MS.gene49468	MS.gene77154	PPI
MS.gene77154	MS.gene070944	PPI
MS.gene95802	MS.gene77154	PPI
MS.gene34370	MS.gene77154	PPI
MS.gene77154	MS.gene071361	PPI
MS.gene49472	MS.gene77154	PPI
MS.gene77154	MS.gene49470	PPI
MS.gene77154	MS.gene49468	PPI
MS.gene77154	MS.gene006417	PPI
MS.gene77154	MS.gene74082	PPI
MS.gene001038	MS.gene77154	PPI
MS.gene77154	MS.gene024857	PPI
MS.gene21830	MS.gene77154	PPI
MS.gene21833	MS.gene77154	PPI
MS.gene043815	MS.gene77154	PPI
MS.gene77154	MS.gene95801	PPI
MS.gene77154	MS.gene001037	PPI
MS.gene77154	MS.gene21833	PPI
MS.gene024858	MS.gene77154	PPI
MS.gene024855	MS.gene77154	PPI
MS.gene006418	MS.gene77154	PPI
MS.gene77154	MS.gene024859	PPI
MS.gene77154	MS.gene95802	PPI
MS.gene77154	MS.gene001032	PPI
MS.gene77154	MS.gene49471	PPI
MS.gene77154	MS.gene006418	PPI
MS.gene29937	MS.gene77154	PPI
MS.gene77154	MS.gene001038	PPI
MS.gene77154	MS.gene071357	PPI
MS.gene77154	MS.gene050493	PPI
MS.gene77154	MS.gene21828	PPI
MS.gene77154	MS.gene070945	PPI
MS.gene001033	MS.gene77154	PPI
MS.gene77154	MS.gene001033	PPI
MS.gene77154	MS.gene024854	PPI
MS.gene77154	MS.gene22888	PPI
MS.gene77154	MS.gene04530	PPI
MS.gene024854	MS.gene77154	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77154.t1	MTR_5g006450	85.057	174	25	1	1	173	1	174	2.35e-103	295
MS.gene77154.t1	MTR_5g006470	69.540	174	50	2	1	173	1	172	2.01e-76	226
MS.gene77154.t1	MTR_5g006520	72.561	164	44	1	1	163	1719	1882	1.83e-74	243
MS.gene77154.t1	MTR_5g006510	68.293	164	44	4	1	162	7	164	7.79e-68	205
MS.gene77154.t1	MTR_5g006460	68.452	168	45	6	1	162	1	166	5.23e-66	200
MS.gene77154.t1	MTR_1g103110	59.394	165	62	3	1	162	1	163	1.12e-60	187
MS.gene77154.t1	MTR_1g103150	56.970	165	66	3	1	162	1	163	1.39e-57	179
MS.gene77154.t1	MTR_7g114410	35.294	136	85	1	16	151	3	135	3.51e-27	100
MS.gene77154.t1	MTR_7g114410	35.294	136	85	1	16	151	3	135	7.46e-27	100
MS.gene77154.t1	MTR_3g463610	33.813	139	92	0	17	155	3	141	1.55e-26	100
MS.gene77154.t1	MTR_1g103320	44.538	119	49	3	53	170	13	115	4.36e-23	88.6
MS.gene77154.t1	MTR_7g024765	32.026	153	98	3	20	171	7	154	2.69e-22	88.6
MS.gene77154.t1	MTR_7g024760	36.765	136	81	3	20	155	7	137	1.06e-21	87.0
MS.gene77154.t1	MTR_7g024780	32.026	153	99	3	17	168	4	152	1.93e-20	83.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77154.t1	AT2G32030	46.104	154	68	4	3	155	14	153	8.11e-40	134
MS.gene77154.t1	AT2G32020	47.794	136	64	2	21	155	19	148	3.38e-36	124
MS.gene77154.t1	AT3G22560	33.793	145	90	3	21	162	7	148	1.87e-20	83.6

Find 43 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCAAGGTCAGAAAGATTA+AGG	0.227074	2.4:+32047839	None:intergenic
CTCTTCTTTGTCACGTGGTT+TGG	0.270188	2.4:+32047894	None:intergenic
ATGTTCTAGGCTCTAAATAT+TGG	0.292142	2.4:-32047600	MS.gene77154:CDS
GCAGAACTTGGCTATGTTCT+AGG	0.302641	2.4:-32047613	MS.gene77154:CDS
TAAGGGGTCGGAGTGTGATT+TGG	0.307480	2.4:+32047857	None:intergenic
TGTTCTAGGCTCTAAATATT+GGG	0.316974	2.4:-32047599	MS.gene77154:CDS
AAGGGGTCGGAGTGTGATTT+GGG	0.320072	2.4:+32047858	None:intergenic
ATATAGCTTCAAAGTTTCTA+TGG	0.332117	2.4:-32047723	MS.gene77154:CDS
TCCATCAATGATCGTGCAAT+TGG	0.335248	2.4:-32047688	MS.gene77154:CDS
CCCAATTGCACGATCATTGA+TGG	0.338330	2.4:+32047687	None:intergenic
AATGTTGGGTCTCAGAGTTT+TGG	0.354229	2.4:-32047478	MS.gene77154:CDS
GCTCTAAATATTGGGGTAAA+GGG	0.356917	2.4:-32047591	MS.gene77154:CDS
GGCTCTAAATATTGGGGTAA+AGG	0.422886	2.4:-32047592	MS.gene77154:CDS
AGTTGCCAAGTTCTGCTCTT+GGG	0.428431	2.4:-32047788	MS.gene77154:CDS
GTTGATGCCATCATCCTTGC+TGG	0.429573	2.4:+32047756	None:intergenic
CACTTGTTTCACAACACACT+TGG	0.439429	2.4:+32047564	None:intergenic
ATTCTGTGAGTTGTCTTACT+TGG	0.446468	2.4:-32047530	MS.gene77154:CDS
AAGTTGCCAAGTTCTGCTCT+TGG	0.477212	2.4:-32047789	MS.gene77154:CDS
AGGAAATATTTGTTCATGAA+AGG	0.480337	2.4:-32047423	MS.gene77154:CDS
CCATCAATGATCGTGCAATT+GGG	0.483539	2.4:-32047687	MS.gene77154:CDS
GTGAGTTGTCTTACTTGGAA+AGG	0.487957	2.4:-32047525	MS.gene77154:CDS
GCTTGAAGCTTTAGTTGATG+TGG	0.488968	2.4:-32047503	MS.gene77154:CDS
TTCTGACCTTGATGATCTCA+TGG	0.490514	2.4:-32047830	MS.gene77154:CDS
ATCAAGGTCAGAAAGATTAA+GGG	0.492658	2.4:+32047840	None:intergenic
TAGTTGATGTGGAAAATGTT+GGG	0.494202	2.4:-32047492	MS.gene77154:CDS
TCGTTCCTTGATTTATCACC+AGG	0.504570	2.4:+32047640	None:intergenic
GTTCACCTGGTGATAAATCA+AGG	0.507986	2.4:-32047645	MS.gene77154:CDS
TTAGTTGATGTGGAAAATGT+TGG	0.523001	2.4:-32047493	MS.gene77154:CDS
AGGAACGAATGTGCAGAACT+TGG	0.523520	2.4:-32047625	MS.gene77154:CDS
ACCTTGATGATCTCATGGTA+TGG	0.538119	2.4:-32047825	MS.gene77154:CDS
TGTTGTGAAACAAGTGGTTA+AGG	0.541729	2.4:-32047557	MS.gene77154:CDS
TCTCTATCCTCAAGTTCACC+TGG	0.545696	2.4:-32047658	MS.gene77154:CDS
CTCTACACCAGCAAGGATGA+TGG	0.559987	2.4:-32047763	MS.gene77154:CDS
CTCTAAATATTGGGGTAAAG+GGG	0.564900	2.4:-32047590	MS.gene77154:CDS
GAGTTCCCAAGAGCAGAACT+TGG	0.571214	2.4:+32047783	None:intergenic
TTTATCACCAGGTGAACTTG+AGG	0.579933	2.4:+32047651	None:intergenic
ATTCTCTCTTCTTTGTCACG+TGG	0.580393	2.4:+32047889	None:intergenic
GGTCAGAAAGATTAAGGGGT+CGG	0.585362	2.4:+32047845	None:intergenic
GTTCTAGGCTCTAAATATTG+GGG	0.619040	2.4:-32047598	MS.gene77154:CDS
TCCATACCATGAGATCATCA+AGG	0.632686	2.4:+32047824	None:intergenic
TCAAGGTCAGAAAGATTAAG+GGG	0.633672	2.4:+32047841	None:intergenic
TTGGGAACTCTACACCAGCA+AGG	0.679749	2.4:-32047770	MS.gene77154:CDS
CAAGTGTGTTGTGAAACAAG+TGG	0.740021	2.4:-32047563	MS.gene77154:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGGAAATATTTGTTCATGAA+AGG	-	chr2.4:32047907-32047926	MS.gene77154:CDS	25.0%
!	ATATAGCTTCAAAGTTTCTA+TGG	-	chr2.4:32047607-32047626	MS.gene77154:CDS	25.0%
	ATCAAGGTCAGAAAGATTAA+GGG	+	chr2.4:32047493-32047512	None:intergenic	30.0%
!	TAGTTGATGTGGAAAATGTT+GGG	-	chr2.4:32047838-32047857	MS.gene77154:CDS	30.0%
!	TTAGTTGATGTGGAAAATGT+TGG	-	chr2.4:32047837-32047856	MS.gene77154:CDS	30.0%
!!	ATGTTCTAGGCTCTAAATAT+TGG	-	chr2.4:32047730-32047749	MS.gene77154:CDS	30.0%
!!	TGTTCTAGGCTCTAAATATT+GGG	-	chr2.4:32047731-32047750	MS.gene77154:CDS	30.0%
	ATTCTGTGAGTTGTCTTACT+TGG	-	chr2.4:32047800-32047819	MS.gene77154:CDS	35.0%
	CATCAAGGTCAGAAAGATTA+AGG	+	chr2.4:32047494-32047513	None:intergenic	35.0%
	CTCTAAATATTGGGGTAAAG+GGG	-	chr2.4:32047740-32047759	MS.gene77154:CDS	35.0%
	TCAAGGTCAGAAAGATTAAG+GGG	+	chr2.4:32047492-32047511	None:intergenic	35.0%
	TGTTGTGAAACAAGTGGTTA+AGG	-	chr2.4:32047773-32047792	MS.gene77154:CDS	35.0%
!	AAAGCTGGTTTCCAAAAAGA+GGG	-	chr2.4:32047877-32047896	MS.gene77154:CDS	35.0%
!	GCTCTAAATATTGGGGTAAA+GGG	-	chr2.4:32047739-32047758	MS.gene77154:CDS	35.0%
!!	GTTCTAGGCTCTAAATATTG+GGG	-	chr2.4:32047732-32047751	MS.gene77154:CDS	35.0%
	ATTCTCTCTTCTTTGTCACG+TGG	+	chr2.4:32047444-32047463	None:intergenic	40.0%
	CAAGTGTGTTGTGAAACAAG+TGG	-	chr2.4:32047767-32047786	MS.gene77154:CDS	40.0%
	CACTTGTTTCACAACACACT+TGG	+	chr2.4:32047769-32047788	None:intergenic	40.0%
	CCATCAATGATCGTGCAATT+GGG	-	chr2.4:32047643-32047662	MS.gene77154:CDS	40.0%
	GTGAGTTGTCTTACTTGGAA+AGG	-	chr2.4:32047805-32047824	MS.gene77154:CDS	40.0%
	GTTCACCTGGTGATAAATCA+AGG	-	chr2.4:32047685-32047704	MS.gene77154:CDS	40.0%
	TCCAAAAAGAGGGAGTTCTA+AGG	-	chr2.4:32047887-32047906	MS.gene77154:CDS	40.0%
	TCCATACCATGAGATCATCA+AGG	+	chr2.4:32047509-32047528	None:intergenic	40.0%
	TCCATCAATGATCGTGCAAT+TGG	-	chr2.4:32047642-32047661	MS.gene77154:CDS	40.0%
	TCGTTCCTTGATTTATCACC+AGG	+	chr2.4:32047693-32047712	None:intergenic	40.0%
	TTTATCACCAGGTGAACTTG+AGG	+	chr2.4:32047682-32047701	None:intergenic	40.0%
!	AATGTTGGGTCTCAGAGTTT+TGG	-	chr2.4:32047852-32047871	MS.gene77154:CDS	40.0%
!	ACCTTGATGATCTCATGGTA+TGG	-	chr2.4:32047505-32047524	MS.gene77154:CDS	40.0%
!	GAAAGCTGGTTTCCAAAAAG+AGG	-	chr2.4:32047876-32047895	MS.gene77154:CDS	40.0%
!	GCTTGAAGCTTTAGTTGATG+TGG	-	chr2.4:32047827-32047846	MS.gene77154:CDS	40.0%
!	TCCTTAGAACTCCCTCTTTT+TGG	+	chr2.4:32047891-32047910	None:intergenic	40.0%
!	TTCTGACCTTGATGATCTCA+TGG	-	chr2.4:32047500-32047519	MS.gene77154:CDS	40.0%
!!	GGCTCTAAATATTGGGGTAA+AGG	-	chr2.4:32047738-32047757	MS.gene77154:CDS	40.0%
	AAGTTGCCAAGTTCTGCTCT+TGG	-	chr2.4:32047541-32047560	MS.gene77154:CDS	45.0%
	AGGAACGAATGTGCAGAACT+TGG	-	chr2.4:32047705-32047724	MS.gene77154:CDS	45.0%
	AGTTGCCAAGTTCTGCTCTT+GGG	-	chr2.4:32047542-32047561	MS.gene77154:CDS	45.0%
	CCCAATTGCACGATCATTGA+TGG	+	chr2.4:32047646-32047665	None:intergenic	45.0%
	CTCTTCTTTGTCACGTGGTT+TGG	+	chr2.4:32047439-32047458	None:intergenic	45.0%
	GCAGAACTTGGCTATGTTCT+AGG	-	chr2.4:32047717-32047736	MS.gene77154:CDS	45.0%
	GGTCAGAAAGATTAAGGGGT+CGG	+	chr2.4:32047488-32047507	None:intergenic	45.0%
	TCTCTATCCTCAAGTTCACC+TGG	-	chr2.4:32047672-32047691	MS.gene77154:CDS	45.0%
!!!	CTCAGAGTTTTGGAGAAAGC+TGG	-	chr2.4:32047862-32047881	MS.gene77154:CDS	45.0%
	AAGGGGTCGGAGTGTGATTT+GGG	+	chr2.4:32047475-32047494	None:intergenic	50.0%
	CTCTACACCAGCAAGGATGA+TGG	-	chr2.4:32047567-32047586	MS.gene77154:CDS	50.0%
	GAGTTCCCAAGAGCAGAACT+TGG	+	chr2.4:32047550-32047569	None:intergenic	50.0%
	GTTGATGCCATCATCCTTGC+TGG	+	chr2.4:32047577-32047596	None:intergenic	50.0%
	TAAGGGGTCGGAGTGTGATT+TGG	+	chr2.4:32047476-32047495	None:intergenic	50.0%
	TTGGGAACTCTACACCAGCA+AGG	-	chr2.4:32047560-32047579	MS.gene77154:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	32047414	32047938	32047414	ID=MS.gene77154
chr2.4	mRNA	32047414	32047938	32047414	ID=MS.gene77154.t1;Parent=MS.gene77154
chr2.4	exon	32047414	32047938	32047414	ID=MS.gene77154.t1.exon1;Parent=MS.gene77154.t1
chr2.4	CDS	32047414	32047938	32047414	ID=cds.MS.gene77154.t1;Parent=MS.gene77154.t1

Gene Sequence

>MS.gene77154

ATGGAGATAACTTCTATTTCTTCCAAACCACGTGACAAAGAAGAGAGAATTGATCTAACCCAAATCACACTCCGACCCCTTAATCTTTCTGACCTTGATGATCTCATGGTATGGACAACTGATGAAAAAGTTGCCAAGTTCTGCTCTTGGGAACTCTACACCAGCAAGGATGATGGCATCAACTTCATTGAAAATATAGCTTCAAAGTTTCTATGGTGCAAAGCAATATCCATCAATGATCGTGCAATTGGGTGTGTTTCTCTATCCTCAAGTTCACCTGGTGATAAATCAAGGAACGAATGTGCAGAACTTGGCTATGTTCTAGGCTCTAAATATTGGGGTAAAGGGGTTGCCAAGTGTGTTGTGAAACAAGTGGTTAAGGCTGCATTCTGTGAGTTGTCTTACTTGGAAAGGCTTGAAGCTTTAGTTGATGTGGAAAATGTTGGGTCTCAGAGTTTTGGAGAAAGCTGGTTTCCAAAAAGAGGGAGTTCTAAGGAAATATTTGTTCATGAAAGGAAAAAGTAG

Protein sequence

>MS.gene77154.t1

MEITSISSKPRDKEERIDLTQITLRPLNLSDLDDLMVWTTDEKVAKFCSWELYTSKDDGINFIENIASKFLWCKAISINDRAIGCVSLSSSSPGDKSRNECAELGYVLGSKYWGKGVAKCVVKQVVKAAFCELSYLERLEALVDVENVGSQSFGESWFPKRGSSKEIFVHERKK