AlfalfaGEDB | MS.gene043815

M. sativa cultivar XinJiangDaYe / MS.gene043815

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043815.t1	XP_003610737.1	95.3	190	9	0	1	190	1	190	1.00E-95	359.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043815.t1	P05332	31.2	176	110	3	15	184	2	172	9.5e-13	75.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043815.t1	G7K3P2	95.3	190	9	0	1	190	1	190	7.5e-96	359.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene043815.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene043815	MS.gene21830	PPI
MS.gene043815	MS.gene050493	PPI
MS.gene043815	MS.gene001034	PPI
MS.gene043815	MS.gene024855	PPI
MS.gene043815	MS.gene070945	PPI
MS.gene043815	MS.gene024854	PPI
MS.gene043815	MS.gene22888	PPI
MS.gene043815	MS.gene04530	PPI
MS.gene043815	MS.gene043813	PPI
MS.gene043815	MS.gene21831	PPI
MS.gene043815	MS.gene024612	PPI
MS.gene043815	MS.gene34371	PPI
MS.gene043815	MS.gene006417	PPI
MS.gene043815	MS.gene49472	PPI
MS.gene043815	MS.gene21827	PPI
MS.gene043815	MS.gene024858	PPI
MS.gene043815	MS.gene070944	PPI
MS.gene043815	MS.gene071361	PPI
MS.gene043815	MS.gene34370	PPI
MS.gene024855	MS.gene043815	PPI
MS.gene043815	MS.gene49470	PPI
MS.gene043815	MS.gene77154	PPI
MS.gene043815	MS.gene29937	PPI
MS.gene043815	MS.gene49468	PPI
MS.gene043815	MS.gene74082	PPI
MS.gene043815	MS.gene024857	PPI
MS.gene043815	MS.gene95801	PPI
MS.gene043815	MS.gene001037	PPI
MS.gene043815	MS.gene21833	PPI
MS.gene024858	MS.gene043815	PPI
MS.gene043815	MS.gene071357	PPI
MS.gene043815	MS.gene21828	PPI
MS.gene043815	MS.gene001033	PPI
MS.gene043815	MS.gene024859	PPI
MS.gene043815	MS.gene95802	PPI
MS.gene043815	MS.gene001032	PPI
MS.gene043815	MS.gene49471	PPI
MS.gene043815	MS.gene006418	PPI
MS.gene043815	MS.gene024614	PPI
MS.gene043815	MS.gene001038	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043815.t1	MTR_5g006450	95.263	190	9	0	1	190	1	190	3.03e-131	366
MS.gene043815.t1	MTR_5g006470	77.895	190	40	1	1	190	1	188	1.11e-101	291
MS.gene043815.t1	MTR_5g006520	79.474	190	39	0	1	190	1719	1908	1.30e-98	312
MS.gene043815.t1	MTR_5g006520	53.333	75	12	1	97	171	1655	1706	9.81e-13	66.2
MS.gene043815.t1	MTR_5g006510	74.346	191	42	3	1	190	7	191	1.58e-96	278
MS.gene043815.t1	MTR_5g006460	73.846	195	44	5	1	190	1	193	3.02e-92	267
MS.gene043815.t1	MTR_1g103110	63.542	192	66	2	1	190	1	190	1.54e-84	248
MS.gene043815.t1	MTR_1g103150	59.896	192	73	2	1	190	1	190	5.80e-79	234
MS.gene043815.t1	MTR_7g114410	42.105	171	96	1	17	187	4	171	1.66e-45	148
MS.gene043815.t1	MTR_3g463610	38.728	173	106	0	17	189	3	175	2.38e-43	144
MS.gene043815.t1	MTR_7g114410	42.308	156	87	1	17	172	4	156	3.76e-42	139
MS.gene043815.t1	MTR_7g024760	41.667	168	93	3	20	187	7	169	4.69e-38	129
MS.gene043815.t1	MTR_7g024780	39.535	172	100	2	16	187	3	170	2.71e-37	127
MS.gene043815.t1	MTR_7g024765	39.286	168	97	3	20	187	7	169	4.18e-36	124
MS.gene043815.t1	MTR_1g103320	55.128	78	33	1	53	130	13	88	3.59e-20	81.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043815.t1	AT2G32030	49.457	184	78	4	5	187	16	185	8.50e-59	182
MS.gene043815.t1	AT2G32020	50.595	168	76	2	21	187	19	180	1.22e-53	169
MS.gene043815.t1	AT3G22560	39.080	174	101	2	16	187	2	172	4.50e-37	127

Find 44 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCAAGGTCAGAAAGATTA+AGG	0.227074	2.2:+27837120	None:intergenic
TGTTGGGTCTCAAAGAGTTT+TGG	0.282550	2.2:-27836757	MS.gene043815:CDS
ATGTTCTAGGCTCTAAATAT+TGG	0.292142	2.2:-27836881	MS.gene043815:CDS
GCAGAACTTGGCTATGTTCT+AGG	0.302641	2.2:-27836894	MS.gene043815:CDS
TAAGGGGTCGGAGTGTGATT+TGG	0.307480	2.2:+27837138	None:intergenic
CTCTTCTTTGTCACGTGGTT+TGG	0.315246	2.2:+27837175	None:intergenic
TGTTCTAGGCTCTAAATATT+GGG	0.316974	2.2:-27836880	MS.gene043815:CDS
AAGGGGTCGGAGTGTGATTT+GGG	0.320072	2.2:+27837139	None:intergenic
GCTCTAAATATTGGGGTAAA+GGG	0.356917	2.2:-27836872	MS.gene043815:CDS
ATATAGCTACTAAGTTTCTA+TGG	0.387531	2.2:-27837004	MS.gene043815:CDS
CCAAACAATTGAATTATAGT+TGG	0.393357	2.2:+27836634	None:intergenic
TGCATCAATGATCGTGCAAT+TGG	0.403589	2.2:-27836969	MS.gene043815:CDS
GGCTCTAAATATTGGGGTAA+AGG	0.422886	2.2:-27836873	MS.gene043815:CDS
TGTTGTGAAGCAAGTGGTTA+AGG	0.443085	2.2:-27836838	MS.gene043815:CDS
ATTCTGTGAGTTGTCTTACT+TGG	0.446468	2.2:-27836811	MS.gene043815:CDS
GTTGATGCCATCATCCTTGC+TGG	0.459050	2.2:+27837037	None:intergenic
AGTGGCCAAGTTCTGCTCTT+GGG	0.462852	2.2:-27837069	MS.gene043815:CDS
AAGTGGCCAAGTTCTGCTCT+TGG	0.474319	2.2:-27837070	MS.gene043815:CDS
AGGAAATATTTGTTCATGAA+AGG	0.480337	2.2:-27836702	MS.gene043815:CDS
AGGAACAAATGTGCAGAACT+TGG	0.481600	2.2:-27836906	MS.gene043815:CDS
GTGAGTTGTCTTACTTGGAA+AGG	0.487957	2.2:-27836806	MS.gene043815:CDS
GCTTGAAGCTTTAGTTGATG+TGG	0.488968	2.2:-27836784	MS.gene043815:CDS
TTCTGACCTTGATGATCTCA+TGG	0.490514	2.2:-27837111	MS.gene043815:CDS
ATCAAGGTCAGAAAGATTAA+GGG	0.492658	2.2:+27837121	None:intergenic
TAGTTGATGTGGAAAATGTT+GGG	0.494202	2.2:-27836773	MS.gene043815:CDS
TTGTTCCTTGATTTATCACC+AGG	0.502130	2.2:+27836921	None:intergenic
GTTCACCTGGTGATAAATCA+AGG	0.507986	2.2:-27836926	MS.gene043815:CDS
TTAGTTGATGTGGAAAATGT+TGG	0.523001	2.2:-27836774	MS.gene043815:CDS
ACCTTGATGATCTCATGGTA+TGG	0.538119	2.2:-27837106	MS.gene043815:CDS
TCTCTATCCTCAAGTTCACC+TGG	0.545696	2.2:-27836939	MS.gene043815:CDS
CTCTACGCCAGCAAGGATGA+TGG	0.563263	2.2:-27837044	MS.gene043815:CDS
CTCTAAATATTGGGGTAAAG+GGG	0.564900	2.2:-27836871	MS.gene043815:CDS
GCATCAATGATCGTGCAATT+GGG	0.567462	2.2:-27836968	MS.gene043815:CDS
GAGTTCCCAAGAGCAGAACT+TGG	0.571214	2.2:+27837064	None:intergenic
TTTATCACCAGGTGAACTTG+AGG	0.579933	2.2:+27836932	None:intergenic
GGTCAGAAAGATTAAGGGGT+CGG	0.585362	2.2:+27837126	None:intergenic
ATTATCTCTTCTTTGTCACG+TGG	0.596771	2.2:+27837170	None:intergenic
GTTCTAGGCTCTAAATATTG+GGG	0.619040	2.2:-27836879	MS.gene043815:CDS
TCCATACCATGAGATCATCA+AGG	0.632686	2.2:+27837105	None:intergenic
GAGATCAGTAAAGAGAACAC+TGG	0.633341	2.2:+27836656	None:intergenic
TCAAGGTCAGAAAGATTAAG+GGG	0.633672	2.2:+27837122	None:intergenic
TTGGGAACTCTACGCCAGCA+AGG	0.634354	2.2:-27837051	MS.gene043815:CDS
CACTTGCTTCACAACACACG+TGG	0.636822	2.2:+27836845	None:intergenic
CACGTGTGTTGTGAAGCAAG+TGG	0.722407	2.2:-27836844	MS.gene043815:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGGAAATATTTGTTCATGAA+AGG	-	chr2.2:27837142-27837161	MS.gene043815:CDS	25.0%
!	ATATAGCTACTAAGTTTCTA+TGG	-	chr2.2:27836840-27836859	MS.gene043815:CDS	25.0%
	ATCAAGGTCAGAAAGATTAA+GGG	+	chr2.2:27836726-27836745	None:intergenic	30.0%
!	TAGTTGATGTGGAAAATGTT+GGG	-	chr2.2:27837071-27837090	MS.gene043815:CDS	30.0%
!	TTAGTTGATGTGGAAAATGT+TGG	-	chr2.2:27837070-27837089	MS.gene043815:CDS	30.0%
!!	ATGTTCTAGGCTCTAAATAT+TGG	-	chr2.2:27836963-27836982	MS.gene043815:CDS	30.0%
!!	TGTTCTAGGCTCTAAATATT+GGG	-	chr2.2:27836964-27836983	MS.gene043815:CDS	30.0%
	ATGGACAACTGATGAAAAAG+TGG	-	chr2.2:27836757-27836776	MS.gene043815:CDS	35.0%
	ATTATCTCTTCTTTGTCACG+TGG	+	chr2.2:27836677-27836696	None:intergenic	35.0%
	ATTCTGTGAGTTGTCTTACT+TGG	-	chr2.2:27837033-27837052	MS.gene043815:CDS	35.0%
	CATCAAGGTCAGAAAGATTA+AGG	+	chr2.2:27836727-27836746	None:intergenic	35.0%
	CTCTAAATATTGGGGTAAAG+GGG	-	chr2.2:27836973-27836992	MS.gene043815:CDS	35.0%
	TCAAGGTCAGAAAGATTAAG+GGG	+	chr2.2:27836725-27836744	None:intergenic	35.0%
	TTGTTCCTTGATTTATCACC+AGG	+	chr2.2:27836926-27836945	None:intergenic	35.0%
!	AAAGCTGGTTTCCAAAAAGA+GGG	-	chr2.2:27837112-27837131	MS.gene043815:CDS	35.0%
!	GCTCTAAATATTGGGGTAAA+GGG	-	chr2.2:27836972-27836991	MS.gene043815:CDS	35.0%
!!	GTTCTAGGCTCTAAATATTG+GGG	-	chr2.2:27836965-27836984	MS.gene043815:CDS	35.0%
	AGGAACAAATGTGCAGAACT+TGG	-	chr2.2:27836938-27836957	MS.gene043815:CDS	40.0%
	GAGATCAGTAAAGAGAACAC+TGG	+	chr2.2:27837191-27837210	None:intergenic	40.0%
	GCATCAATGATCGTGCAATT+GGG	-	chr2.2:27836876-27836895	MS.gene043815:CDS	40.0%
	GTGAGTTGTCTTACTTGGAA+AGG	-	chr2.2:27837038-27837057	MS.gene043815:CDS	40.0%
	GTTCACCTGGTGATAAATCA+AGG	-	chr2.2:27836918-27836937	MS.gene043815:CDS	40.0%
	TCCAAAAAGAGGGAGTTCTA+AGG	-	chr2.2:27837122-27837141	MS.gene043815:CDS	40.0%
	TCCATACCATGAGATCATCA+AGG	+	chr2.2:27836742-27836761	None:intergenic	40.0%
	TGCATCAATGATCGTGCAAT+TGG	-	chr2.2:27836875-27836894	MS.gene043815:CDS	40.0%
	TGTTGTGAAGCAAGTGGTTA+AGG	-	chr2.2:27837006-27837025	MS.gene043815:CDS	40.0%
	TTTATCACCAGGTGAACTTG+AGG	+	chr2.2:27836915-27836934	None:intergenic	40.0%
!	ACCTTGATGATCTCATGGTA+TGG	-	chr2.2:27836738-27836757	MS.gene043815:CDS	40.0%
!	GAAAGCTGGTTTCCAAAAAG+AGG	-	chr2.2:27837111-27837130	MS.gene043815:CDS	40.0%
!	GCTTGAAGCTTTAGTTGATG+TGG	-	chr2.2:27837060-27837079	MS.gene043815:CDS	40.0%
!	TCCTTAGAACTCCCTCTTTT+TGG	+	chr2.2:27837126-27837145	None:intergenic	40.0%
!	TGTTGGGTCTCAAAGAGTTT+TGG	-	chr2.2:27837087-27837106	MS.gene043815:CDS	40.0%
!	TTCTGACCTTGATGATCTCA+TGG	-	chr2.2:27836733-27836752	MS.gene043815:CDS	40.0%
!!	GGCTCTAAATATTGGGGTAA+AGG	-	chr2.2:27836971-27836990	MS.gene043815:CDS	40.0%
!!!	CAAAGAGTTTTGGAGAAAGC+TGG	-	chr2.2:27837097-27837116	MS.gene043815:CDS	40.0%
	CTCTTCTTTGTCACGTGGTT+TGG	+	chr2.2:27836672-27836691	None:intergenic	45.0%
	GCAGAACTTGGCTATGTTCT+AGG	-	chr2.2:27836950-27836969	MS.gene043815:CDS	45.0%
	GGTCAGAAAGATTAAGGGGT+CGG	+	chr2.2:27836721-27836740	None:intergenic	45.0%
	TCTCTATCCTCAAGTTCACC+TGG	-	chr2.2:27836905-27836924	MS.gene043815:CDS	45.0%
	AAGGGGTCGGAGTGTGATTT+GGG	+	chr2.2:27836708-27836727	None:intergenic	50.0%
	AAGTGGCCAAGTTCTGCTCT+TGG	-	chr2.2:27836774-27836793	MS.gene043815:CDS	50.0%
	AGTGGCCAAGTTCTGCTCTT+GGG	-	chr2.2:27836775-27836794	MS.gene043815:CDS	50.0%
	CACGTGTGTTGTGAAGCAAG+TGG	-	chr2.2:27837000-27837019	MS.gene043815:CDS	50.0%
	CACTTGCTTCACAACACACG+TGG	+	chr2.2:27837002-27837021	None:intergenic	50.0%
	GAGTTCCCAAGAGCAGAACT+TGG	+	chr2.2:27836783-27836802	None:intergenic	50.0%
	GTTGATGCCATCATCCTTGC+TGG	+	chr2.2:27836810-27836829	None:intergenic	50.0%
	TAAGGGGTCGGAGTGTGATT+TGG	+	chr2.2:27836709-27836728	None:intergenic	50.0%
	CTCTACGCCAGCAAGGATGA+TGG	-	chr2.2:27836800-27836819	MS.gene043815:CDS	55.0%
	TTGGGAACTCTACGCCAGCA+AGG	-	chr2.2:27836793-27836812	MS.gene043815:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	27836647	27837219	27836647	ID=MS.gene043815
chr2.2	mRNA	27836647	27837219	27836647	ID=MS.gene043815.t1;Parent=MS.gene043815
chr2.2	exon	27836647	27837219	27836647	ID=MS.gene043815.t1.exon1;Parent=MS.gene043815.t1
chr2.2	CDS	27836647	27837219	27836647	ID=cds.MS.gene043815.t1;Parent=MS.gene043815.t1

Gene Sequence

>MS.gene043815

ATGGAGATAATTTCTATTTCTTCCAAACCACGTGACAAAGAAGAGATAATTGATCTAACCCAAATCACACTCCGACCCCTTAATCTTTCTGACCTTGATGATCTCATGGTATGGACAACTGATGAAAAAGTGGCCAAGTTCTGCTCTTGGGAACTCTACGCCAGCAAGGATGATGGCATCAACTTCATTGAAAATATAGCTACTAAGTTTCTATGGTGCAAAGCAATATGCATCAATGATCGTGCAATTGGGTGTGTTTCTCTATCCTCAAGTTCACCTGGTGATAAATCAAGGAACAAATGTGCAGAACTTGGCTATGTTCTAGGCTCTAAATATTGGGGTAAAGGGGTTGCCACGTGTGTTGTGAAGCAAGTGGTTAAGGTTGCATTCTGTGAGTTGTCTTACTTGGAAAGGCTTGAAGCTTTAGTTGATGTGGAAAATGTTGGGTCTCAAAGAGTTTTGGAGAAAGCTGGTTTCCAAAAAGAGGGAGTTCTAAGGAAATATTTGTTCATGAAAGGAAAAAGTAGAGATATGATCATCTCCAGTGTTCTCTTTACTGATCTCCAACTATAA

Protein sequence

>MS.gene043815.t1

MEIISISSKPRDKEEIIDLTQITLRPLNLSDLDDLMVWTTDEKVAKFCSWELYASKDDGINFIENIATKFLWCKAICINDRAIGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVATCVVKQVVKVAFCELSYLERLEALVDVENVGSQRVLEKAGFQKEGVLRKYLFMKGKSRDMIISSVLFTDLQL