AlfalfaGEDB | MS.gene006417

M. sativa cultivar XinJiangDaYe / MS.gene006417

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006417.t1	XP_013469780.1	84.7	190	5	1	2	167	1	190	2.30E-78	301.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006417.t1	P05332	32.0	169	86	2	24	164	8	175	1.2e-14	81.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006417.t1	A0A072VQW9	84.7	190	5	1	2	167	1	190	1.6e-78	301.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene006417.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene006417	MS.gene49470	PPI
MS.gene006417	MS.gene74082	PPI
MS.gene006417	MS.gene49471	PPI
MS.gene006417	MS.gene024857	PPI
MS.gene071361	MS.gene006417	PPI
MS.gene070945	MS.gene006417	PPI
MS.gene006417	MS.gene95801	PPI
MS.gene043815	MS.gene006417	PPI
MS.gene21828	MS.gene006417	PPI
MS.gene95802	MS.gene006417	PPI
MS.gene34370	MS.gene006417	PPI
MS.gene49472	MS.gene006417	PPI
MS.gene006417	MS.gene071357	PPI
MS.gene024859	MS.gene006417	PPI
MS.gene024855	MS.gene006417	PPI
MS.gene77154	MS.gene006417	PPI
MS.gene024612	MS.gene006417	PPI
MS.gene006417	MS.gene001032	PPI
MS.gene34371	MS.gene006417	PPI
MS.gene006417	MS.gene04530	PPI
MS.gene001038	MS.gene006417	PPI
MS.gene006417	MS.gene024614	PPI
MS.gene21830	MS.gene006417	PPI
MS.gene21833	MS.gene006417	PPI
MS.gene29937	MS.gene006417	PPI
MS.gene024858	MS.gene006417	PPI
MS.gene006417	MS.gene050493	PPI
MS.gene006417	MS.gene001034	PPI
MS.gene006418	MS.gene006417	PPI
MS.gene006417	MS.gene22888	PPI
MS.gene006417	MS.gene043813	PPI
MS.gene024854	MS.gene006417	PPI
MS.gene006417	MS.gene21831	PPI
MS.gene001037	MS.gene006417	PPI
MS.gene006417	MS.gene21827	PPI
MS.gene001033	MS.gene006417	PPI
MS.gene006417	MS.gene070944	PPI
MS.gene21827	MS.gene006417	PPI
MS.gene49468	MS.gene006417	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006417.t1	MTR_1g103110	84.737	190	5	1	2	167	1	190	3.76e-110	311
MS.gene006417.t1	MTR_1g103150	75.263	190	23	1	2	167	1	190	9.66e-98	280
MS.gene006417.t1	MTR_5g006450	58.854	192	51	2	2	167	1	190	1.02e-74	222
MS.gene006417.t1	MTR_5g006470	60.000	190	50	2	2	167	1	188	1.52e-74	221
MS.gene006417.t1	MTR_5g006520	56.477	193	56	2	1	167	1718	1908	1.39e-67	222
MS.gene006417.t1	MTR_5g006460	57.143	196	51	6	2	167	1	193	4.61e-66	200
MS.gene006417.t1	MTR_5g006510	51.546	194	59	5	1	167	6	191	6.02e-62	189
MS.gene006417.t1	MTR_7g114410	42.262	168	74	1	20	164	4	171	5.02e-41	136
MS.gene006417.t1	MTR_3g463610	38.728	173	80	1	20	166	3	175	4.86e-39	132
MS.gene006417.t1	MTR_7g114410	42.484	153	65	1	20	149	4	156	2.57e-36	123
MS.gene006417.t1	MTR_7g024760	41.718	163	74	2	23	164	7	169	5.43e-36	123
MS.gene006417.t1	MTR_7g024765	39.877	163	77	1	23	164	7	169	2.99e-35	121
MS.gene006417.t1	MTR_7g024780	37.870	169	83	3	18	164	2	170	1.85e-33	117
MS.gene006417.t1	MTR_1g103320	42.718	103	34	3	54	131	11	113	1.80e-14	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006417.t1	AT2G32020	48.466	163	63	1	23	164	18	180	1.42e-51	163
MS.gene006417.t1	AT2G32030	43.478	184	82	2	2	164	3	185	1.83e-50	160
MS.gene006417.t1	AT3G22560	38.596	171	80	1	19	164	2	172	6.36e-36	123

Find 33 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTTCTTTAGCATCTGATT+TGG	0.255230	1.3:+70869975	None:intergenic
CCGTCTTCTTTGTTTGTATA+AGG	0.260514	1.3:+70869838	None:intergenic
TGCTATGAGCTCCAAGTATT+GGG	0.261207	1.3:-70869682	MS.gene006417:CDS
TTCAGATCTTGATGATGTTT+TGG	0.285412	1.3:-70869905	MS.gene006417:CDS
GAGATTTGCTTGCTATGTTT+TGG	0.329625	1.3:+70869803	None:intergenic
AAATGGGTCGAAGAGATATT+TGG	0.334810	1.3:+70869932	None:intergenic
TTTAGCATCTGATTTGGAAT+AGG	0.367598	1.3:+70869981	None:intergenic
ATCAAGATCTGAAAGACAAA+TGG	0.391931	1.3:+70869915	None:intergenic
AATACCTTTGCCCCAATACT+TGG	0.403153	1.3:+70869671	None:intergenic
CAGAGGGTGTTGGAGAAGGC+TGG	0.411839	1.3:-70869549	MS.gene006417:CDS
ACATAGCAAGCAAATCTCTA+TGG	0.412043	1.3:-70869798	MS.gene006417:intron
AGGAACAAATCTGCTGAACT+TGG	0.442081	1.3:-70869708	MS.gene006417:CDS
ATGCTATGAGCTCCAAGTAT+TGG	0.448265	1.3:-70869683	MS.gene006417:CDS
CTTGAGCTTCAAGCCGCTCC+AGG	0.458231	1.3:+70869595	None:intergenic
TTCTCAGAGGGTGTTGGAGA+AGG	0.470170	1.3:-70869553	MS.gene006417:CDS
CAGAAAATATCTGTTCATCA+AGG	0.472630	1.3:-70869505	MS.gene006417:CDS
GCTTCAAGCCGCTCCAGGTA+TGG	0.487071	1.3:+70869600	None:intergenic
TCAAGATCTGAAAGACAAAT+GGG	0.525500	1.3:+70869916	None:intergenic
AGGTTCCCAAGTGCAATACT+TGG	0.527166	1.3:+70869858	None:intergenic
AGCTCCAAGTATTGGGGCAA+AGG	0.537498	1.3:-70869675	MS.gene006417:CDS
TAGAAAATGTGGCTTCTCAG+AGG	0.540854	1.3:-70869566	MS.gene006417:CDS
ATTTAAAGAGTTTCCATACC+TGG	0.542410	1.3:-70869613	MS.gene006417:CDS
TGTGGCTTCTCAGAGGGTGT+TGG	0.549890	1.3:-70869559	MS.gene006417:CDS
AGAAAATATCTGTTCATCAA+GGG	0.554840	1.3:-70869504	MS.gene006417:CDS
TCAAGTTGATGTAGAAAATG+TGG	0.572167	1.3:-70869577	MS.gene006417:CDS
AAGAGTTTCCATACCTGGAG+CGG	0.585589	1.3:-70869608	MS.gene006417:CDS
CACACTTGTTGTCAAACAAG+TGG	0.596019	1.3:-70869646	MS.gene006417:CDS
AAGTGGCCAAGTATTGCACT+TGG	0.601796	1.3:-70869864	MS.gene006417:CDS
AGAAAATGTGGCTTCTCAGA+GGG	0.613906	1.3:-70869565	MS.gene006417:CDS
CACTTGTTTGACAACAAGTG+TGG	0.622968	1.3:+70869647	None:intergenic
CCTTATACAAACAAAGAAGA+CGG	0.623002	1.3:-70869838	MS.gene006417:CDS
GCTATGAGCTCCAAGTATTG+GGG	0.656803	1.3:-70869681	MS.gene006417:CDS
AGTGGCCAAGTATTGCACTT+GGG	0.670287	1.3:-70869863	MS.gene006417:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGAAAATATCTGTTCATCAA+GGG	-	chr1.3:70869945-70869964	MS.gene006417:CDS	25.0%
	ATCAAGATCTGAAAGACAAA+TGG	+	chr1.3:70869537-70869556	None:intergenic	30.0%
	ATTTAAAGAGTTTCCATACC+TGG	-	chr1.3:70869836-70869855	MS.gene006417:CDS	30.0%
	CAGAAAATATCTGTTCATCA+AGG	-	chr1.3:70869944-70869963	MS.gene006417:CDS	30.0%
	CCTTATACAAACAAAGAAGA+CGG	-	chr1.3:70869611-70869630	MS.gene006417:CDS	30.0%
	TCAAGATCTGAAAGACAAAT+GGG	+	chr1.3:70869536-70869555	None:intergenic	30.0%
!!	TCAAGTTGATGTAGAAAATG+TGG	-	chr1.3:70869872-70869891	MS.gene006417:CDS	30.0%
!!	TGTATCGATTTCTTTGTGTT+CGG	-	chr1.3:70869710-70869729	MS.gene006417:intron	30.0%
!!	TTTAGCATCTGATTTGGAAT+AGG	+	chr1.3:70869471-70869490	None:intergenic	30.0%
!!!	TTCAGATCTTGATGATGTTT+TGG	-	chr1.3:70869544-70869563	MS.gene006417:CDS	30.0%
	AAATGGGTCGAAGAGATATT+TGG	+	chr1.3:70869520-70869539	None:intergenic	35.0%
	ACATAGCAAGCAAATCTCTA+TGG	-	chr1.3:70869651-70869670	MS.gene006417:CDS	35.0%
	CAAGGGAAAAAGTAGAGATA+TGG	-	chr1.3:70869962-70869981	MS.gene006417:CDS	35.0%
	CTCTTCTTTAGCATCTGATT+TGG	+	chr1.3:70869477-70869496	None:intergenic	35.0%
!	CCGTCTTCTTTGTTTGTATA+AGG	+	chr1.3:70869614-70869633	None:intergenic	35.0%
!!!	ATCTTGATGATGTTTTGGTG+TGG	-	chr1.3:70869549-70869568	MS.gene006417:CDS	35.0%
!!!	GAGATTTGCTTGCTATGTTT+TGG	+	chr1.3:70869649-70869668	None:intergenic	35.0%
	AATACCTTTGCCCCAATACT+TGG	+	chr1.3:70869781-70869800	None:intergenic	40.0%
	AGAAAATGTGGCTTCTCAGA+GGG	-	chr1.3:70869884-70869903	MS.gene006417:CDS	40.0%
	AGGAACAAATCTGCTGAACT+TGG	-	chr1.3:70869741-70869760	MS.gene006417:intron	40.0%
	ATGCTATGAGCTCCAAGTAT+TGG	-	chr1.3:70869766-70869785	MS.gene006417:intron	40.0%
	CACTTGTTTGACAACAAGTG+TGG	+	chr1.3:70869805-70869824	None:intergenic	40.0%
	GTGGACAAGTGATGAAAAAG+TGG	-	chr1.3:70869568-70869587	MS.gene006417:CDS	40.0%
	TAGAAAATGTGGCTTCTCAG+AGG	-	chr1.3:70869883-70869902	MS.gene006417:CDS	40.0%
	TGCTATGAGCTCCAAGTATT+GGG	-	chr1.3:70869767-70869786	MS.gene006417:intron	40.0%
!!	CACACTTGTTGTCAAACAAG+TGG	-	chr1.3:70869803-70869822	MS.gene006417:CDS	40.0%
	AAGAGTTTCCATACCTGGAG+CGG	-	chr1.3:70869841-70869860	MS.gene006417:CDS	45.0%
	AGGTTCCCAAGTGCAATACT+TGG	+	chr1.3:70869594-70869613	None:intergenic	45.0%
	CTTTGTGTTCGGATAGATGC+AGG	-	chr1.3:70869721-70869740	MS.gene006417:intron	45.0%
!	AAGTGGCCAAGTATTGCACT+TGG	-	chr1.3:70869585-70869604	MS.gene006417:CDS	45.0%
!	AGTGGCCAAGTATTGCACTT+GGG	-	chr1.3:70869586-70869605	MS.gene006417:CDS	45.0%
!	GCTATGAGCTCCAAGTATTG+GGG	-	chr1.3:70869768-70869787	MS.gene006417:intron	45.0%
!!!	AAGGCTGGTTTTCAAAGGGA+GGG	-	chr1.3:70869915-70869934	MS.gene006417:CDS	45.0%
!!!	GGAGAAGGCTGGTTTTCAAA+GGG	-	chr1.3:70869911-70869930	MS.gene006417:CDS	45.0%
!!!	TGGAGAAGGCTGGTTTTCAA+AGG	-	chr1.3:70869910-70869929	MS.gene006417:CDS	45.0%
!!	AGCTCCAAGTATTGGGGCAA+AGG	-	chr1.3:70869774-70869793	MS.gene006417:intron	50.0%
!!	TTCTCAGAGGGTGTTGGAGA+AGG	-	chr1.3:70869896-70869915	MS.gene006417:CDS	50.0%
!!!	GAAGGCTGGTTTTCAAAGGG+AGG	-	chr1.3:70869914-70869933	MS.gene006417:CDS	50.0%
	TGCCTTCGCGATCGAGCAAT+CGG	-	chr1.3:70869686-70869705	MS.gene006417:CDS	55.0%
!	TGTGGCTTCTCAGAGGGTGT+TGG	-	chr1.3:70869890-70869909	MS.gene006417:CDS	55.0%
	ACCCGATTGCTCGATCGCGA+AGG	+	chr1.3:70869691-70869710	None:intergenic	60.0%
	CTTGAGCTTCAAGCCGCTCC+AGG	+	chr1.3:70869857-70869876	None:intergenic	60.0%
	GCCTTCGCGATCGAGCAATC+GGG	-	chr1.3:70869687-70869706	MS.gene006417:CDS	60.0%
	GCTTCAAGCCGCTCCAGGTA+TGG	+	chr1.3:70869852-70869871	None:intergenic	60.0%
!!	CAGAGGGTGTTGGAGAAGGC+TGG	-	chr1.3:70869900-70869919	MS.gene006417:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	70869449	70870022	70869449	ID=MS.gene006417
chr1.3	mRNA	70869449	70870022	70869449	ID=MS.gene006417.t1;Parent=MS.gene006417
chr1.3	exon	70869799	70870022	70869799	ID=MS.gene006417.t1.exon1;Parent=MS.gene006417.t1
chr1.3	CDS	70869799	70870022	70869799	ID=cds.MS.gene006417.t1;Parent=MS.gene006417.t1
chr1.3	exon	70869449	70869728	70869449	ID=MS.gene006417.t1.exon2;Parent=MS.gene006417.t1
chr1.3	CDS	70869449	70869728	70869449	ID=cds.MS.gene006417.t1;Parent=MS.gene006417.t1

Gene Sequence

>MS.gene006417

ATGATGGAAAAAACTGCTACCTATTCCAAATCAGATGCTAAAGAAGAGTGTTCTGTAGTTGACTTGAGCCAAATATCTCTTCGACCCATTTGTCTTTCAGATCTTGATGATGTTTTGGTGTGGACAAGTGATGAAAAAGTGGCCAAGTATTGCACTTGGGAACCTTATACAAACAAAGAAGACGGCATTGACTTCATCCAAAACATAGCAAGCAAATCTCTATGGTTCAGAGCAATTTGCCTTCGCGATCGAGCAATCGGGTGTATCGATTTCTTTGTGTTCGGATAGATGCAGGAACAAATCTGCTGAACTTGGCTATGCTATGAGCTCCAAGTATTGGGGCAAAGGTATTGCCACACTTGTTGTCAAACAAGTGGTTGAAGCTGCATTTAAAGAGTTTCCATACCTGGAGCGGCTTGAAGCTCAAGTTGATGTAGAAAATGTGGCTTCTCAGAGGGTGTTGGAGAAGGCTGGTTTTCAAAGGGAGGGTGTTCTCAGAAAATATCTGTTCATCAAGGGAAAAAGTAGAGATATGGTTATGTTCAGTATTCTCTCAAGTGAAGTTCAAATTTGA

Protein sequence

>MS.gene006417.t1

MMEKTATYSKSDAKEECSVVDLSQISLRPICLSDLDDVLVWTSDEKVAKYCTWEPYTNKEDGIDFIQNIASKSLWNKSAELGYAMSSKYWGKGIATLVVKQVVEAAFKEFPYLERLEAQVDVENVASQRVLEKAGFQREGVLRKYLFIKGKSRDMVMFSILSSEVQI