AlfalfaGEDB | MS.gene006418

M. sativa cultivar XinJiangDaYe / MS.gene006418

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006418.t1	XP_013469781.1	96.8	185	6	0	2	186	1	185	1.00E-95	359.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006418.t1	P05332	33.9	168	102	3	24	185	8	172	3.3e-18	93.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006418.t1	A0A072VP71	96.8	185	6	0	2	186	1	185	7.5e-96	359.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene006418.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene006418	MS.gene001037	PPI
MS.gene006418	MS.gene21833	PPI
MS.gene006418	MS.gene001038	PPI
MS.gene006418	MS.gene071357	PPI
MS.gene006418	MS.gene050493	PPI
MS.gene006418	MS.gene21828	PPI
MS.gene006418	MS.gene001033	PPI
MS.gene006418	MS.gene95802	PPI
MS.gene006418	MS.gene024854	PPI
MS.gene006418	MS.gene22888	PPI
MS.gene006418	MS.gene04530	PPI
MS.gene001033	MS.gene006418	PPI
MS.gene006418	MS.gene024614	PPI
MS.gene006418	MS.gene024612	PPI
MS.gene49468	MS.gene006418	PPI
MS.gene024854	MS.gene006418	PPI
MS.gene006418	MS.gene21830	PPI
MS.gene006418	MS.gene001034	PPI
MS.gene006418	MS.gene070945	PPI
MS.gene006418	MS.gene071361	PPI
MS.gene006418	MS.gene043813	PPI
MS.gene006418	MS.gene21831	PPI
MS.gene006418	MS.gene77154	PPI
MS.gene95802	MS.gene006418	PPI
MS.gene34370	MS.gene006418	PPI
MS.gene006418	MS.gene34371	PPI
MS.gene006418	MS.gene006417	PPI
MS.gene49472	MS.gene006418	PPI
MS.gene006418	MS.gene21827	PPI
MS.gene006418	MS.gene024857	PPI
MS.gene77154	MS.gene006418	PPI
MS.gene006418	MS.gene070944	PPI
MS.gene006418	MS.gene95801	PPI
MS.gene001038	MS.gene006418	PPI
MS.gene21830	MS.gene006418	PPI
MS.gene21833	MS.gene006418	PPI
MS.gene006418	MS.gene49470	PPI
MS.gene043815	MS.gene006418	PPI
MS.gene006418	MS.gene49468	PPI
MS.gene006418	MS.gene024859	PPI
MS.gene29937	MS.gene006418	PPI
MS.gene006418	MS.gene001032	PPI
MS.gene024858	MS.gene006418	PPI
MS.gene006418	MS.gene74082	PPI
MS.gene006418	MS.gene49471	PPI
MS.gene024855	MS.gene006418	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006418.t1	MTR_1g103150	96.757	185	6	0	2	186	1	185	6.90e-133	370
MS.gene006418.t1	MTR_1g103110	84.865	185	28	0	2	186	1	185	3.68e-114	323
MS.gene006418.t1	MTR_5g006470	62.766	188	68	1	2	189	1	186	3.92e-81	239
MS.gene006418.t1	MTR_5g006450	62.903	186	65	2	2	185	1	184	1.24e-80	238
MS.gene006418.t1	MTR_5g006520	59.686	191	73	2	1	189	1718	1906	4.14e-72	236
MS.gene006418.t1	MTR_5g006460	58.763	194	71	6	2	189	1	191	1.63e-68	207
MS.gene006418.t1	MTR_5g006510	52.356	191	86	3	2	189	1	189	8.26e-67	203
MS.gene006418.t1	MTR_7g114410	48.235	170	83	2	20	187	4	170	1.20e-48	156
MS.gene006418.t1	MTR_3g463610	44.186	172	88	2	20	186	3	171	6.91e-46	150
MS.gene006418.t1	MTR_7g114410	48.718	156	75	2	20	173	4	156	4.34e-44	144
MS.gene006418.t1	MTR_7g024765	42.683	164	91	3	23	186	7	167	4.57e-38	129
MS.gene006418.t1	MTR_7g024760	42.424	165	92	3	23	187	7	168	1.19e-37	128
MS.gene006418.t1	MTR_7g024780	39.881	168	99	2	18	185	2	167	2.18e-37	127
MS.gene006418.t1	MTR_1g103320	57.282	103	43	1	54	155	11	113	1.22e-33	116

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006418.t1	AT2G32020	52.727	165	73	3	23	186	18	178	1.27e-54	172
MS.gene006418.t1	AT2G32030	45.989	187	96	3	1	186	1	183	2.19e-54	171
MS.gene006418.t1	AT3G22560	41.420	169	92	2	19	183	2	167	4.18e-36	124

Find 47 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGAAAATATCTGTTCATTA+AGG	0.174922	1.3:-70871354	MS.gene006418:CDS
TGTGGTTGAAGCTGCATTTA+AGG	0.195666	1.3:-70871477	MS.gene006418:CDS
TTCTTTGAGCTCCATGTATT+GGG	0.213171	1.3:-70871531	MS.gene006418:CDS
CTCTTCTTTAGCATCTGATT+TGG	0.248198	1.3:+70871826	None:intergenic
ATTCTTTGAGCTCCATGTAT+TGG	0.293943	1.3:-70871532	MS.gene006418:CDS
GCCTCAAGCCTCTCCAAGTA+TGG	0.329009	1.3:+70871449	None:intergenic
GAGATTTGCTTGCTATGTTT+TGG	0.329625	1.3:+70871654	None:intergenic
CCTTATACAAACAAAGAAAA+AGG	0.343403	1.3:-70871689	MS.gene006418:CDS
TGCCTTCGCGATAGAGCAAT+TGG	0.346097	1.3:-70871614	MS.gene006418:CDS
GGGCAAAGGGATTGCCACTA+TGG	0.352044	1.3:-70871510	MS.gene006418:CDS
TCAAGATCAGAAAGACTAAT+TGG	0.352059	1.3:+70871767	None:intergenic
TTTAGCATCTGATTTGGAAT+AGG	0.367598	1.3:+70871832	None:intergenic
AATTGGGTGTACCGAGTTCC+AGG	0.375481	1.3:-70871597	MS.gene006418:CDS
AGAAAATATCTGTTCATTAA+GGG	0.384110	1.3:-70871353	MS.gene006418:CDS
ATTTAAGGAGTTTCCATACT+TGG	0.408342	1.3:-70871462	MS.gene006418:CDS
ACATAGCAAGCAAATCTCTA+TGG	0.412043	1.3:-70871649	MS.gene006418:CDS
CAAAGGGTGTTGGAGAAGGC+TGG	0.437360	1.3:-70871398	MS.gene006418:CDS
TACCGAGTTCCAGGTGTGTT+CGG	0.452367	1.3:-70871588	MS.gene006418:CDS
TAATTGGTCGAAGAGAGATT+TGG	0.457252	1.3:+70871783	None:intergenic
CGGGACAAATCTGCTCAACT+TGG	0.459831	1.3:-70871557	MS.gene006418:CDS
GGCATCTATCCGAACACACC+TGG	0.486937	1.3:+70871579	None:intergenic
AGCTCCATGTATTGGGGCAA+AGG	0.494926	1.3:-70871524	MS.gene006418:CDS
AATCCCTTTGCCCCAATACA+TGG	0.496567	1.3:+70871520	None:intergenic
AGGTGTGTTCGGATAGATGC+CGG	0.506000	1.3:-70871577	MS.gene006418:CDS
TTCTCAAAGGGTGTTGGAGA+AGG	0.511828	1.3:-70871402	MS.gene006418:CDS
GCCTTCGCGATAGAGCAATT+GGG	0.515394	1.3:-70871613	MS.gene006418:CDS
GCTCCATGTATTGGGGCAAA+GGG	0.517436	1.3:-70871523	MS.gene006418:CDS
ATCTTGATGATGTTATGTTG+TGG	0.524636	1.3:-70871751	MS.gene006418:CDS
AGGTTCCCAAGTGCAATACT+TGG	0.527166	1.3:+70871709	None:intergenic
CAAGTTGAGCAGATTTGTCC+CGG	0.527345	1.3:+70871558	None:intergenic
GAAAATATCTGTTCATTAAG+GGG	0.536787	1.3:-70871352	MS.gene006418:CDS
TGTGGCTTCTCAAAGGGTGT+TGG	0.546349	1.3:-70871408	MS.gene006418:CDS
TCCATACTTGGAGAGGCTTG+AGG	0.557426	1.3:-70871450	MS.gene006418:CDS
CACATGTTTCACAACCATAG+TGG	0.561077	1.3:+70871496	None:intergenic
GCTTGAGGCTCGAGTTGATG+TGG	0.569120	1.3:-70871435	MS.gene006418:CDS
TGGAAAATGTGGCTTCTCAA+AGG	0.572207	1.3:-70871415	MS.gene006418:CDS
AGGAGTTTCCATACTTGGAG+AGG	0.574247	1.3:-70871457	MS.gene006418:CDS
ATCCGAACACACCTGGAACT+CGG	0.577234	1.3:+70871586	None:intergenic
TCTTTGAGCTCCATGTATTG+GGG	0.587210	1.3:-70871530	MS.gene006418:CDS
GGAAAATGTGGCTTCTCAAA+GGG	0.588500	1.3:-70871414	MS.gene006418:CDS
AAGTGGCCAAGTATTGCACT+TGG	0.595225	1.3:-70871715	MS.gene006418:CDS
GGTGTGTTCGGATAGATGCC+GGG	0.599178	1.3:-70871576	MS.gene006418:CDS
GTGGACTAGTGATGCAAAAG+TGG	0.603948	1.3:-70871732	MS.gene006418:CDS
ACCCAATTGCTCTATCGCGA+AGG	0.616201	1.3:+70871612	None:intergenic
TCGAGTTGATGTGGAAAATG+TGG	0.669414	1.3:-70871426	MS.gene006418:CDS
AGTGGCCAAGTATTGCACTT+GGG	0.670287	1.3:-70871714	MS.gene006418:CDS
CACTATGGTTGTGAAACATG+TGG	0.680456	1.3:-70871495	MS.gene006418:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGAAAATATCTGTTCATTAA+GGG	-	chr1.3:70871802-70871821	MS.gene006418:CDS	20.0%
!	CAGAAAATATCTGTTCATTA+AGG	-	chr1.3:70871801-70871820	MS.gene006418:CDS	25.0%
!	CCTTATACAAACAAAGAAAA+AGG	-	chr1.3:70871466-70871485	MS.gene006418:CDS	25.0%
!	GAAAATATCTGTTCATTAAG+GGG	-	chr1.3:70871803-70871822	MS.gene006418:CDS	25.0%
!!!	CCTTTTTCTTTGTTTGTATA+AGG	+	chr1.3:70871469-70871488	None:intergenic	25.0%
	ATTTAAGGAGTTTCCATACT+TGG	-	chr1.3:70871693-70871712	MS.gene006418:CDS	30.0%
	TCAAGATCAGAAAGACTAAT+TGG	+	chr1.3:70871391-70871410	None:intergenic	30.0%
!!	ATCTTGATGATGTTATGTTG+TGG	-	chr1.3:70871404-70871423	MS.gene006418:CDS	30.0%
!!	TTTAGCATCTGATTTGGAAT+AGG	+	chr1.3:70871326-70871345	None:intergenic	30.0%
	ACATAGCAAGCAAATCTCTA+TGG	-	chr1.3:70871506-70871525	MS.gene006418:CDS	35.0%
	ATTCTTTGAGCTCCATGTAT+TGG	-	chr1.3:70871623-70871642	MS.gene006418:CDS	35.0%
	CTCTTCTTTAGCATCTGATT+TGG	+	chr1.3:70871332-70871351	None:intergenic	35.0%
	TAATTGGTCGAAGAGAGATT+TGG	+	chr1.3:70871375-70871394	None:intergenic	35.0%
	TTCTTTGAGCTCCATGTATT+GGG	-	chr1.3:70871624-70871643	MS.gene006418:CDS	35.0%
!!!	GAGATTTGCTTGCTATGTTT+TGG	+	chr1.3:70871504-70871523	None:intergenic	35.0%
	CACATGTTTCACAACCATAG+TGG	+	chr1.3:70871662-70871681	None:intergenic	40.0%
	CACTATGGTTGTGAAACATG+TGG	-	chr1.3:70871660-70871679	MS.gene006418:CDS	40.0%
	GGAAAATGTGGCTTCTCAAA+GGG	-	chr1.3:70871741-70871760	MS.gene006418:CDS	40.0%
	TCTTTGAGCTCCATGTATTG+GGG	-	chr1.3:70871625-70871644	MS.gene006418:CDS	40.0%
	TGGAAAATGTGGCTTCTCAA+AGG	-	chr1.3:70871740-70871759	MS.gene006418:CDS	40.0%
	TGTGGTTGAAGCTGCATTTA+AGG	-	chr1.3:70871678-70871697	MS.gene006418:CDS	40.0%
!!	TCGAGTTGATGTGGAAAATG+TGG	-	chr1.3:70871729-70871748	MS.gene006418:CDS	40.0%
	AATCCCTTTGCCCCAATACA+TGG	+	chr1.3:70871638-70871657	None:intergenic	45.0%
	AGGAGTTTCCATACTTGGAG+AGG	-	chr1.3:70871698-70871717	MS.gene006418:CDS	45.0%
	AGGTTCCCAAGTGCAATACT+TGG	+	chr1.3:70871449-70871468	None:intergenic	45.0%
!	AAGTGGCCAAGTATTGCACT+TGG	-	chr1.3:70871440-70871459	MS.gene006418:CDS	45.0%
!	AGTGGCCAAGTATTGCACTT+GGG	-	chr1.3:70871441-70871460	MS.gene006418:CDS	45.0%
!	CAAGTTGAGCAGATTTGTCC+CGG	+	chr1.3:70871600-70871619	None:intergenic	45.0%
!!	GTGGACTAGTGATGCAAAAG+TGG	-	chr1.3:70871423-70871442	MS.gene006418:CDS	45.0%
!!	TTCTCAAAGGGTGTTGGAGA+AGG	-	chr1.3:70871753-70871772	MS.gene006418:CDS	45.0%
!!!	AAGGCTGGTTTTGAAAGGGA+AGG	-	chr1.3:70871772-70871791	MS.gene006418:CDS	45.0%
!!!	GGAGAAGGCTGGTTTTGAAA+GGG	-	chr1.3:70871768-70871787	MS.gene006418:CDS	45.0%
!!!	TGGAGAAGGCTGGTTTTGAA+AGG	-	chr1.3:70871767-70871786	MS.gene006418:CDS	45.0%
	AATTGGGTGTACCGAGTTCC+AGG	-	chr1.3:70871558-70871577	MS.gene006418:CDS	50.0%
	ACCCAATTGCTCTATCGCGA+AGG	+	chr1.3:70871546-70871565	None:intergenic	50.0%
	AGCTCCATGTATTGGGGCAA+AGG	-	chr1.3:70871631-70871650	MS.gene006418:CDS	50.0%
	AGGTGTGTTCGGATAGATGC+CGG	-	chr1.3:70871578-70871597	MS.gene006418:CDS	50.0%
	ATCCGAACACACCTGGAACT+CGG	+	chr1.3:70871572-70871591	None:intergenic	50.0%
	CGGGACAAATCTGCTCAACT+TGG	-	chr1.3:70871598-70871617	MS.gene006418:CDS	50.0%
	GCCTTCGCGATAGAGCAATT+GGG	-	chr1.3:70871542-70871561	MS.gene006418:CDS	50.0%
	GCTCCATGTATTGGGGCAAA+GGG	-	chr1.3:70871632-70871651	MS.gene006418:CDS	50.0%
	TACCGAGTTCCAGGTGTGTT+CGG	-	chr1.3:70871567-70871586	MS.gene006418:CDS	50.0%
	TCCATACTTGGAGAGGCTTG+AGG	-	chr1.3:70871705-70871724	MS.gene006418:CDS	50.0%
	TGCCTTCGCGATAGAGCAAT+TGG	-	chr1.3:70871541-70871560	MS.gene006418:CDS	50.0%
!	TGTGGCTTCTCAAAGGGTGT+TGG	-	chr1.3:70871747-70871766	MS.gene006418:CDS	50.0%
	GCCTCAAGCCTCTCCAAGTA+TGG	+	chr1.3:70871709-70871728	None:intergenic	55.0%
	GGCATCTATCCGAACACACC+TGG	+	chr1.3:70871579-70871598	None:intergenic	55.0%
	GGGCAAAGGGATTGCCACTA+TGG	-	chr1.3:70871645-70871664	MS.gene006418:CDS	55.0%
	GGTGTGTTCGGATAGATGCC+GGG	-	chr1.3:70871579-70871598	MS.gene006418:CDS	55.0%
!!	CAAAGGGTGTTGGAGAAGGC+TGG	-	chr1.3:70871757-70871776	MS.gene006418:CDS	55.0%
!!	GCTTGAGGCTCGAGTTGATG+TGG	-	chr1.3:70871720-70871739	MS.gene006418:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	70871304	70871873	70871304	ID=MS.gene006418
chr1.3	mRNA	70871304	70871873	70871304	ID=MS.gene006418.t1;Parent=MS.gene006418
chr1.3	exon	70871304	70871873	70871304	ID=MS.gene006418.t1.exon1;Parent=MS.gene006418.t1
chr1.3	CDS	70871304	70871873	70871304	ID=cds.MS.gene006418.t1;Parent=MS.gene006418.t1

Gene Sequence

>MS.gene006418

ATGATGGAAGAAACTGCTACCTATTCCAAATCAGATGCTAAAGAAGAGTATTCTGTTGTTGACTTGAGCCAAATCTCTCTTCGACCAATTAGTCTTTCTGATCTTGATGATGTTATGTTGTGGACTAGTGATGCAAAAGTGGCCAAGTATTGCACTTGGGAACCTTATACAAACAAAGAAAAAGGCATTGACTTCATCCAAAACATAGCAAGCAAATCTCTATGGTTCAGAGCAATTTGCCTTCGCGATAGAGCAATTGGGTGTACCGAGTTCCAGGTGTGTTCGGATAGATGCCGGGACAAATCTGCTCAACTTGGCTATTCTTTGAGCTCCATGTATTGGGGCAAAGGGATTGCCACTATGGTTGTGAAACATGTGGTTGAAGCTGCATTTAAGGAGTTTCCATACTTGGAGAGGCTTGAGGCTCGAGTTGATGTGGAAAATGTGGCTTCTCAAAGGGTGTTGGAGAAGGCTGGTTTTGAAAGGGAAGGTGTTCTCAGAAAATATCTGTTCATTAAGGGGAAAAGTATAGATATTGTTATGTTCAGTATTCTCTCAGTGAAGTTATAA

Protein sequence

>MS.gene006418.t1

MMEETATYSKSDAKEEYSVVDLSQISLRPISLSDLDDVMLWTSDAKVAKYCTWEPYTNKEKGIDFIQNIASKSLWFRAICLRDRAIGCTEFQVCSDRCRDKSAQLGYSLSSMYWGKGIATMVVKHVVEAAFKEFPYLERLEARVDVENVASQRVLEKAGFEREGVLRKYLFIKGKSIDIVMFSILSVKL