AlfalfaGEDB | MS.gene95801

M. sativa cultivar XinJiangDaYe / MS.gene95801

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95801.t1	XP_013469781.1	96.8	185	6	0	1	185	1	185	1.00E-95	359.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95801.t1	P05332	33.9	168	102	3	23	184	8	172	3.3e-18	93.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95801.t1	A0A072VP71	96.8	185	6	0	1	185	1	185	7.4e-96	359.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene95801.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene95801	MS.gene071357	PPI
MS.gene001033	MS.gene95801	PPI
MS.gene21827	MS.gene95801	PPI
MS.gene95801	MS.gene22888	PPI
MS.gene024854	MS.gene95801	PPI
MS.gene95801	MS.gene04530	PPI
MS.gene95801	MS.gene024614	PPI
MS.gene006417	MS.gene95801	PPI
MS.gene95801	MS.gene001034	PPI
MS.gene071361	MS.gene95801	PPI
MS.gene49468	MS.gene95801	PPI
MS.gene21828	MS.gene95801	PPI
MS.gene95802	MS.gene95801	PPI
MS.gene34370	MS.gene95801	PPI
MS.gene95801	MS.gene043813	PPI
MS.gene95801	MS.gene21831	PPI
MS.gene49472	MS.gene95801	PPI
MS.gene77154	MS.gene95801	PPI
MS.gene043813	MS.gene95801	PPI
MS.gene34371	MS.gene95801	PPI
MS.gene001038	MS.gene95801	PPI
MS.gene070945	MS.gene95801	PPI
MS.gene95801	MS.gene024857	PPI
MS.gene21830	MS.gene95801	PPI
MS.gene21833	MS.gene95801	PPI
MS.gene95801	MS.gene070944	PPI
MS.gene043815	MS.gene95801	PPI
MS.gene024858	MS.gene95801	PPI
MS.gene024859	MS.gene95801	PPI
MS.gene024855	MS.gene95801	PPI
MS.gene95801	MS.gene49470	PPI
MS.gene006418	MS.gene95801	PPI
MS.gene024612	MS.gene95801	PPI
MS.gene050493	MS.gene95801	PPI
MS.gene95801	MS.gene001032	PPI
MS.gene95801	MS.gene74082	PPI
MS.gene95801	MS.gene49471	PPI
MS.gene29937	MS.gene95801	PPI
MS.gene001037	MS.gene95801	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95801.t1	MTR_1g103150	96.757	185	6	0	1	185	1	185	7.81e-133	370
MS.gene95801.t1	MTR_1g103110	84.865	185	28	0	1	185	1	185	4.55e-114	322
MS.gene95801.t1	MTR_5g006470	62.766	188	68	1	1	188	1	186	3.17e-81	239
MS.gene95801.t1	MTR_5g006450	62.903	186	65	2	1	184	1	184	1.13e-80	238
MS.gene95801.t1	MTR_5g006520	60.000	190	72	2	1	188	1719	1906	4.18e-72	236
MS.gene95801.t1	MTR_5g006460	58.763	194	71	6	1	188	1	191	1.35e-68	207
MS.gene95801.t1	MTR_5g006510	52.356	191	86	3	1	188	1	189	8.31e-67	202
MS.gene95801.t1	MTR_7g114410	48.521	169	82	2	19	185	4	169	1.25e-48	156
MS.gene95801.t1	MTR_3g463610	44.186	172	88	2	19	185	3	171	7.05e-46	150
MS.gene95801.t1	MTR_7g114410	48.718	156	75	2	19	172	4	156	3.60e-44	144
MS.gene95801.t1	MTR_7g024765	42.683	164	91	3	22	185	7	167	4.77e-38	129
MS.gene95801.t1	MTR_7g024760	42.424	165	92	3	22	186	7	168	1.14e-37	128
MS.gene95801.t1	MTR_7g024780	39.881	168	99	2	17	184	2	167	2.33e-37	127
MS.gene95801.t1	MTR_1g103320	57.282	103	43	1	53	154	11	113	1.30e-33	116

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95801.t1	AT2G32020	52.727	165	73	3	22	185	18	178	1.25e-54	172
MS.gene95801.t1	AT2G32030	46.196	184	94	3	3	185	4	183	2.12e-54	171
MS.gene95801.t1	AT3G22560	41.420	169	92	2	18	182	2	167	4.51e-36	124

Find 47 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGAAAATATCTGTTCATTA+AGG	0.174922	1.1:-74014587	MS.gene95801:CDS
TGTGGTTGAAGCTGCATTTA+AGG	0.195666	1.1:-74014710	MS.gene95801:CDS
TTCTTTGAGCTCCATGTATT+GGG	0.213171	1.1:-74014764	MS.gene95801:CDS
CTCTTCTTTAGCATCTGATT+TGG	0.248198	1.1:+74015059	None:intergenic
ATTCTTTGAGCTCCATGTAT+TGG	0.293943	1.1:-74014765	MS.gene95801:CDS
GCCTCAAGCCTCTCCAAGTA+TGG	0.329009	1.1:+74014682	None:intergenic
GAGATTTGCTTGCTATGTTT+TGG	0.329625	1.1:+74014887	None:intergenic
CCTTATACAAACAAAGAAAA+AGG	0.343403	1.1:-74014922	MS.gene95801:CDS
TGCCTTCGCGATAGAGCAAT+TGG	0.346097	1.1:-74014847	MS.gene95801:CDS
GGGCAAAGGGATTGCCACTA+TGG	0.352044	1.1:-74014743	MS.gene95801:CDS
TCAAGATCAGAAAGACTAAT+TGG	0.352059	1.1:+74015000	None:intergenic
TTTAGCATCTGATTTGGAAT+AGG	0.367598	1.1:+74015065	None:intergenic
AATTGGGTGTACCGAGTTCC+AGG	0.375481	1.1:-74014830	MS.gene95801:CDS
AGAAAATATCTGTTCATTAA+GGG	0.384110	1.1:-74014586	MS.gene95801:CDS
ATTTAAGGAGTTTCCATACT+TGG	0.408342	1.1:-74014695	MS.gene95801:CDS
ACATAGCAAGCAAATCTCTA+TGG	0.412043	1.1:-74014882	MS.gene95801:CDS
CAAAGGGTGTTGGAGAAGGC+TGG	0.437360	1.1:-74014631	MS.gene95801:CDS
TACCGAGTTCCAGGTGTGTT+CGG	0.452367	1.1:-74014821	MS.gene95801:CDS
TAATTGGTCGAAGAGAGATT+TGG	0.457252	1.1:+74015016	None:intergenic
CGGGACAAATCTGCTCAACT+TGG	0.459831	1.1:-74014790	MS.gene95801:CDS
GGCATCTATCCGAACACACC+TGG	0.486937	1.1:+74014812	None:intergenic
AGCTCCATGTATTGGGGCAA+AGG	0.494926	1.1:-74014757	MS.gene95801:CDS
AATCCCTTTGCCCCAATACA+TGG	0.496567	1.1:+74014753	None:intergenic
AGGTGTGTTCGGATAGATGC+CGG	0.506000	1.1:-74014810	MS.gene95801:CDS
TTCTCAAAGGGTGTTGGAGA+AGG	0.511828	1.1:-74014635	MS.gene95801:CDS
GCCTTCGCGATAGAGCAATT+GGG	0.515394	1.1:-74014846	MS.gene95801:CDS
GCTCCATGTATTGGGGCAAA+GGG	0.517436	1.1:-74014756	MS.gene95801:CDS
ATCTTGATGATGTTATGTTG+TGG	0.524636	1.1:-74014984	MS.gene95801:CDS
AGGTTCCCAAGTGCAATACT+TGG	0.527166	1.1:+74014942	None:intergenic
CAAGTTGAGCAGATTTGTCC+CGG	0.527345	1.1:+74014791	None:intergenic
GAAAATATCTGTTCATTAAG+GGG	0.536787	1.1:-74014585	MS.gene95801:CDS
TGTGGCTTCTCAAAGGGTGT+TGG	0.546349	1.1:-74014641	MS.gene95801:CDS
TCCATACTTGGAGAGGCTTG+AGG	0.557426	1.1:-74014683	MS.gene95801:CDS
CACATGTTTCACAACCATAG+TGG	0.561077	1.1:+74014729	None:intergenic
GCTTGAGGCTCGAGTTGATG+TGG	0.569120	1.1:-74014668	MS.gene95801:CDS
TGGAAAATGTGGCTTCTCAA+AGG	0.572207	1.1:-74014648	MS.gene95801:CDS
AGGAGTTTCCATACTTGGAG+AGG	0.574247	1.1:-74014690	MS.gene95801:CDS
ATCCGAACACACCTGGAACT+CGG	0.577234	1.1:+74014819	None:intergenic
TCTTTGAGCTCCATGTATTG+GGG	0.587210	1.1:-74014763	MS.gene95801:CDS
GGAAAATGTGGCTTCTCAAA+GGG	0.588500	1.1:-74014647	MS.gene95801:CDS
AAGTGGCCAAGTATTGCACT+TGG	0.595225	1.1:-74014948	MS.gene95801:CDS
GGTGTGTTCGGATAGATGCC+GGG	0.599178	1.1:-74014809	MS.gene95801:CDS
GTGGACTAGTGATGCAAAAG+TGG	0.603948	1.1:-74014965	MS.gene95801:CDS
ACCCAATTGCTCTATCGCGA+AGG	0.616201	1.1:+74014845	None:intergenic
TCGAGTTGATGTGGAAAATG+TGG	0.669414	1.1:-74014659	MS.gene95801:CDS
AGTGGCCAAGTATTGCACTT+GGG	0.670287	1.1:-74014947	MS.gene95801:CDS
CACTATGGTTGTGAAACATG+TGG	0.680456	1.1:-74014728	MS.gene95801:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGAAAATATCTGTTCATTAA+GGG	-	chr1.1:74015032-74015051	MS.gene95801:CDS	20.0%
!	CAGAAAATATCTGTTCATTA+AGG	-	chr1.1:74015031-74015050	MS.gene95801:CDS	25.0%
!	CCTTATACAAACAAAGAAAA+AGG	-	chr1.1:74014696-74014715	MS.gene95801:CDS	25.0%
!	GAAAATATCTGTTCATTAAG+GGG	-	chr1.1:74015033-74015052	MS.gene95801:CDS	25.0%
!!!	CCTTTTTCTTTGTTTGTATA+AGG	+	chr1.1:74014699-74014718	None:intergenic	25.0%
	ATTTAAGGAGTTTCCATACT+TGG	-	chr1.1:74014923-74014942	MS.gene95801:CDS	30.0%
	TCAAGATCAGAAAGACTAAT+TGG	+	chr1.1:74014621-74014640	None:intergenic	30.0%
!!	ATCTTGATGATGTTATGTTG+TGG	-	chr1.1:74014634-74014653	MS.gene95801:CDS	30.0%
!!	TTTAGCATCTGATTTGGAAT+AGG	+	chr1.1:74014556-74014575	None:intergenic	30.0%
	ACATAGCAAGCAAATCTCTA+TGG	-	chr1.1:74014736-74014755	MS.gene95801:CDS	35.0%
	ATTCTTTGAGCTCCATGTAT+TGG	-	chr1.1:74014853-74014872	MS.gene95801:CDS	35.0%
	CTCTTCTTTAGCATCTGATT+TGG	+	chr1.1:74014562-74014581	None:intergenic	35.0%
	TAATTGGTCGAAGAGAGATT+TGG	+	chr1.1:74014605-74014624	None:intergenic	35.0%
	TTCTTTGAGCTCCATGTATT+GGG	-	chr1.1:74014854-74014873	MS.gene95801:CDS	35.0%
!!!	GAGATTTGCTTGCTATGTTT+TGG	+	chr1.1:74014734-74014753	None:intergenic	35.0%
	CACATGTTTCACAACCATAG+TGG	+	chr1.1:74014892-74014911	None:intergenic	40.0%
	CACTATGGTTGTGAAACATG+TGG	-	chr1.1:74014890-74014909	MS.gene95801:CDS	40.0%
	GGAAAATGTGGCTTCTCAAA+GGG	-	chr1.1:74014971-74014990	MS.gene95801:CDS	40.0%
	TCTTTGAGCTCCATGTATTG+GGG	-	chr1.1:74014855-74014874	MS.gene95801:CDS	40.0%
	TGGAAAATGTGGCTTCTCAA+AGG	-	chr1.1:74014970-74014989	MS.gene95801:CDS	40.0%
	TGTGGTTGAAGCTGCATTTA+AGG	-	chr1.1:74014908-74014927	MS.gene95801:CDS	40.0%
!!	TCGAGTTGATGTGGAAAATG+TGG	-	chr1.1:74014959-74014978	MS.gene95801:CDS	40.0%
	AATCCCTTTGCCCCAATACA+TGG	+	chr1.1:74014868-74014887	None:intergenic	45.0%
	AGGAGTTTCCATACTTGGAG+AGG	-	chr1.1:74014928-74014947	MS.gene95801:CDS	45.0%
	AGGTTCCCAAGTGCAATACT+TGG	+	chr1.1:74014679-74014698	None:intergenic	45.0%
!	AAGTGGCCAAGTATTGCACT+TGG	-	chr1.1:74014670-74014689	MS.gene95801:CDS	45.0%
!	AGTGGCCAAGTATTGCACTT+GGG	-	chr1.1:74014671-74014690	MS.gene95801:CDS	45.0%
!	CAAGTTGAGCAGATTTGTCC+CGG	+	chr1.1:74014830-74014849	None:intergenic	45.0%
!!	GTGGACTAGTGATGCAAAAG+TGG	-	chr1.1:74014653-74014672	MS.gene95801:CDS	45.0%
!!	TTCTCAAAGGGTGTTGGAGA+AGG	-	chr1.1:74014983-74015002	MS.gene95801:CDS	45.0%
!!!	AAGGCTGGTTTTGAAAGGGA+AGG	-	chr1.1:74015002-74015021	MS.gene95801:CDS	45.0%
!!!	GGAGAAGGCTGGTTTTGAAA+GGG	-	chr1.1:74014998-74015017	MS.gene95801:CDS	45.0%
!!!	TGGAGAAGGCTGGTTTTGAA+AGG	-	chr1.1:74014997-74015016	MS.gene95801:CDS	45.0%
	AATTGGGTGTACCGAGTTCC+AGG	-	chr1.1:74014788-74014807	MS.gene95801:CDS	50.0%
	ACCCAATTGCTCTATCGCGA+AGG	+	chr1.1:74014776-74014795	None:intergenic	50.0%
	AGCTCCATGTATTGGGGCAA+AGG	-	chr1.1:74014861-74014880	MS.gene95801:CDS	50.0%
	AGGTGTGTTCGGATAGATGC+CGG	-	chr1.1:74014808-74014827	MS.gene95801:CDS	50.0%
	ATCCGAACACACCTGGAACT+CGG	+	chr1.1:74014802-74014821	None:intergenic	50.0%
	CGGGACAAATCTGCTCAACT+TGG	-	chr1.1:74014828-74014847	MS.gene95801:CDS	50.0%
	GCCTTCGCGATAGAGCAATT+GGG	-	chr1.1:74014772-74014791	MS.gene95801:CDS	50.0%
	GCTCCATGTATTGGGGCAAA+GGG	-	chr1.1:74014862-74014881	MS.gene95801:CDS	50.0%
	TACCGAGTTCCAGGTGTGTT+CGG	-	chr1.1:74014797-74014816	MS.gene95801:CDS	50.0%
	TCCATACTTGGAGAGGCTTG+AGG	-	chr1.1:74014935-74014954	MS.gene95801:CDS	50.0%
	TGCCTTCGCGATAGAGCAAT+TGG	-	chr1.1:74014771-74014790	MS.gene95801:CDS	50.0%
!	TGTGGCTTCTCAAAGGGTGT+TGG	-	chr1.1:74014977-74014996	MS.gene95801:CDS	50.0%
	GCCTCAAGCCTCTCCAAGTA+TGG	+	chr1.1:74014939-74014958	None:intergenic	55.0%
	GGCATCTATCCGAACACACC+TGG	+	chr1.1:74014809-74014828	None:intergenic	55.0%
	GGGCAAAGGGATTGCCACTA+TGG	-	chr1.1:74014875-74014894	MS.gene95801:CDS	55.0%
	GGTGTGTTCGGATAGATGCC+GGG	-	chr1.1:74014809-74014828	MS.gene95801:CDS	55.0%
!!	CAAAGGGTGTTGGAGAAGGC+TGG	-	chr1.1:74014987-74015006	MS.gene95801:CDS	55.0%
!!	GCTTGAGGCTCGAGTTGATG+TGG	-	chr1.1:74014950-74014969	MS.gene95801:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	74014537	74015103	74014537	ID=MS.gene95801
chr1.1	mRNA	74014537	74015103	74014537	ID=MS.gene95801.t1;Parent=MS.gene95801
chr1.1	exon	74014537	74015103	74014537	ID=MS.gene95801.t1.exon1;Parent=MS.gene95801.t1
chr1.1	CDS	74014537	74015103	74014537	ID=cds.MS.gene95801.t1;Parent=MS.gene95801.t1

Gene Sequence

>MS.gene95801

ATGGAAGAAACTGCTACCTATTCCAAATCAGATGCTAAAGAAGAGTATTCTGTTGTTGACTTGAGCCAAATCTCTCTTCGACCAATTAGTCTTTCTGATCTTGATGATGTTATGTTGTGGACTAGTGATGCAAAAGTGGCCAAGTATTGCACTTGGGAACCTTATACAAACAAAGAAAAAGGCATTGACTTCATCCAAAACATAGCAAGCAAATCTCTATGGTTCAGAGCAATTTGCCTTCGCGATAGAGCAATTGGGTGTACCGAGTTCCAGGTGTGTTCGGATAGATGCCGGGACAAATCTGCTCAACTTGGCTATTCTTTGAGCTCCATGTATTGGGGCAAAGGGATTGCCACTATGGTTGTGAAACATGTGGTTGAAGCTGCATTTAAGGAGTTTCCATACTTGGAGAGGCTTGAGGCTCGAGTTGATGTGGAAAATGTGGCTTCTCAAAGGGTGTTGGAGAAGGCTGGTTTTGAAAGGGAAGGTGTTCTCAGAAAATATCTGTTCATTAAGGGGAAAAGTATAGATATTGTTATGTTCAGTATTCTCTCAGTGAAGTTATAA

Protein sequence

>MS.gene95801.t1

MEETATYSKSDAKEEYSVVDLSQISLRPISLSDLDDVMLWTSDAKVAKYCTWEPYTNKEKGIDFIQNIASKSLWFRAICLRDRAIGCTEFQVCSDRCRDKSAQLGYSLSSMYWGKGIATMVVKHVVEAAFKEFPYLERLEARVDVENVASQRVLEKAGFEREGVLRKYLFIKGKSIDIVMFSILSVKL