AlfalfaGEDB | MS.gene071361

M. sativa cultivar XinJiangDaYe / MS.gene071361

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071361.t1	XP_003610737.1	88.1	160	5	1	1	146	1	160	8.10E-72	279.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071361.t1	P05332	29.7	148	87	3	15	146	2	148	7.0e-08	58.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071361.t1	G7K3P2	88.1	160	5	1	1	146	1	160	5.8e-72	279.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene071361.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene071361	MS.gene070944	PPI
MS.gene070945	MS.gene071361	PPI
MS.gene95802	MS.gene071361	PPI
MS.gene34370	MS.gene071361	PPI
MS.gene071361	MS.gene49470	PPI
MS.gene49472	MS.gene071361	PPI
MS.gene024855	MS.gene071361	PPI
MS.gene071361	MS.gene006417	PPI
MS.gene071361	MS.gene74082	PPI
MS.gene071361	MS.gene024857	PPI
MS.gene77154	MS.gene071361	PPI
MS.gene071361	MS.gene95801	PPI
MS.gene001038	MS.gene071361	PPI
MS.gene071361	MS.gene001037	PPI
MS.gene21830	MS.gene071361	PPI
MS.gene21833	MS.gene071361	PPI
MS.gene043815	MS.gene071361	PPI
MS.gene071361	MS.gene071357	PPI
MS.gene071361	MS.gene21828	PPI
MS.gene29937	MS.gene071361	PPI
MS.gene024858	MS.gene071361	PPI
MS.gene024859	MS.gene071361	PPI
MS.gene071361	MS.gene001032	PPI
MS.gene006418	MS.gene071361	PPI
MS.gene071361	MS.gene49471	PPI
MS.gene024854	MS.gene071361	PPI
MS.gene071361	MS.gene050493	PPI
MS.gene071361	MS.gene22888	PPI
MS.gene001033	MS.gene071361	PPI
MS.gene071361	MS.gene04530	PPI
MS.gene49468	MS.gene071361	PPI
MS.gene071361	MS.gene043813	PPI
MS.gene071361	MS.gene024614	PPI
MS.gene071361	MS.gene21831	PPI
MS.gene071361	MS.gene024612	PPI
MS.gene071361	MS.gene34371	PPI
MS.gene071361	MS.gene001034	PPI
MS.gene071361	MS.gene21827	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071361.t1	MTR_5g006450	88.125	160	5	1	1	146	1	160	1.83e-98	281
MS.gene071361.t1	MTR_5g006470	73.418	158	30	1	1	146	1	158	9.87e-77	226
MS.gene071361.t1	MTR_5g006520	73.125	160	29	1	1	146	1719	1878	2.49e-74	241
MS.gene071361.t1	MTR_5g006510	66.460	161	33	4	1	146	7	161	1.29e-65	198
MS.gene071361.t1	MTR_5g006460	67.879	165	32	6	1	146	1	163	6.59e-65	196
MS.gene071361.t1	MTR_1g103110	61.875	160	47	2	1	146	1	160	1.40e-63	193
MS.gene071361.t1	MTR_1g103150	59.375	160	51	2	1	146	1	160	3.00e-61	187
MS.gene071361.t1	MTR_7g114410	39.007	141	75	2	16	145	3	143	1.47e-33	115
MS.gene071361.t1	MTR_7g114410	39.007	141	75	2	16	145	3	143	2.74e-33	115
MS.gene071361.t1	MTR_3g463610	36.364	143	77	2	17	145	3	145	1.00e-29	107
MS.gene071361.t1	MTR_7g024760	40.146	137	71	2	20	146	7	142	6.03e-29	104
MS.gene071361.t1	MTR_7g024765	37.226	137	75	2	20	146	7	142	5.55e-28	102
MS.gene071361.t1	MTR_7g024780	36.170	141	78	2	17	146	4	143	1.05e-24	93.6
MS.gene071361.t1	MTR_1g103320	50.000	76	26	1	53	116	13	88	6.44e-15	66.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071361.t1	AT2G32030	48.026	152	62	3	3	145	14	157	5.02e-45	145
MS.gene071361.t1	AT2G32020	53.731	134	53	2	21	145	19	152	8.11e-44	142
MS.gene071361.t1	AT3G22560	34.965	143	80	3	17	146	3	145	8.84e-25	94.0

Find 37 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCAAGGTCAGAAAGATTA+AGG	0.227074	2.1:+34165408	None:intergenic
CTCTTCTTTGTCACGTGGTT+TGG	0.270188	2.1:+34165463	None:intergenic
TGTTGGGTCTCAAAGAGTTT+TGG	0.282550	2.1:-34165046	MS.gene071361:CDS
ATGTTCTAGGCTCTAAATAT+TGG	0.292142	2.1:-34165170	MS.gene071361:CDS
GCAGAACTTGGCTATGTTCT+AGG	0.302641	2.1:-34165183	MS.gene071361:CDS
TAAGGGGTCGGAGTGTGATT+TGG	0.307480	2.1:+34165426	None:intergenic
TGTTCTAGGCTCTAAATATT+GGG	0.316974	2.1:-34165169	MS.gene071361:CDS
AAGGGGTCGGAGTGTGATTT+GGG	0.320072	2.1:+34165427	None:intergenic
TGCATCAATGATCGTGCAAT+TGG	0.335248	2.1:-34165257	MS.gene071361:CDS
GCTCTAAATATTGGGGTAAA+GGG	0.356917	2.1:-34165161	MS.gene071361:CDS
ATATAGCTACTAAGTTTCTA+TGG	0.387531	2.1:-34165292	MS.gene071361:CDS
GGCTCTAAATATTGGGGTAA+AGG	0.422886	2.1:-34165162	MS.gene071361:CDS
ATTCTGTGAGTTGTCTTACT+TGG	0.446468	2.1:-34165100	MS.gene071361:CDS
GTTGATGCCATCATCCTTGC+TGG	0.459050	2.1:+34165325	None:intergenic
AGTGGCCAAGTTCTGCTCTT+GGG	0.462852	2.1:-34165357	MS.gene071361:CDS
AAGTGGCCAAGTTCTGCTCT+TGG	0.474319	2.1:-34165358	MS.gene071361:CDS
AGGAACAAATGTGCAGAACT+TGG	0.481600	2.1:-34165195	MS.gene071361:CDS
GTGAGTTGTCTTACTTGGAA+AGG	0.487957	2.1:-34165095	MS.gene071361:CDS
GCTTGAAGCTTTAGTTGATG+TGG	0.488968	2.1:-34165073	MS.gene071361:CDS
TTCTGACCTTGATGATCTCA+TGG	0.490514	2.1:-34165399	MS.gene071361:CDS
ATCAAGGTCAGAAAGATTAA+GGG	0.492658	2.1:+34165409	None:intergenic
TAGTTGATGTGGAAAATGTT+GGG	0.494202	2.1:-34165062	MS.gene071361:CDS
GCATCAATGATCGTGCAATT+GGG	0.508704	2.1:-34165256	MS.gene071361:intron
TTAGTTGATGTGGAAAATGT+TGG	0.523001	2.1:-34165063	MS.gene071361:CDS
ACCTTGATGATCTCATGGTA+TGG	0.538119	2.1:-34165394	MS.gene071361:CDS
TGTTGTGAAACAAGTGGTTA+AGG	0.545474	2.1:-34165127	MS.gene071361:CDS
CTCTACGCCAGCAAGGATGA+TGG	0.563263	2.1:-34165332	MS.gene071361:CDS
CTCTAAATATTGGGGTAAAG+GGG	0.564900	2.1:-34165160	MS.gene071361:CDS
GAGTTCCCAAGAGCAGAACT+TGG	0.571214	2.1:+34165352	None:intergenic
ATTCTCTCTTCTTTGTCACG+TGG	0.580393	2.1:+34165458	None:intergenic
GGTCAGAAAGATTAAGGGGT+CGG	0.585362	2.1:+34165414	None:intergenic
CACTTGTTTCACAACACACG+TGG	0.596860	2.1:+34165134	None:intergenic
GTTCTAGGCTCTAAATATTG+GGG	0.619040	2.1:-34165168	MS.gene071361:CDS
TCCATACCATGAGATCATCA+AGG	0.632686	2.1:+34165393	None:intergenic
TCAAGGTCAGAAAGATTAAG+GGG	0.633672	2.1:+34165410	None:intergenic
TTGGGAACTCTACGCCAGCA+AGG	0.634354	2.1:-34165339	MS.gene071361:CDS
CACGTGTGTTGTGAAACAAG+TGG	0.736637	2.1:-34165133	MS.gene071361:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATATAGCTACTAAGTTTCTA+TGG	-	chr2.1:34165219-34165238	MS.gene071361:intron	25.0%
	ATCAAGGTCAGAAAGATTAA+GGG	+	chr2.1:34165105-34165124	None:intergenic	30.0%
	TTTGTTCCTTGATTTATCAC+AGG	+	chr2.1:34165305-34165324	None:intergenic	30.0%
!	TAGTTGATGTGGAAAATGTT+GGG	-	chr2.1:34165449-34165468	MS.gene071361:CDS	30.0%
!	TTAGTTGATGTGGAAAATGT+TGG	-	chr2.1:34165448-34165467	MS.gene071361:CDS	30.0%
!!	ATGTTCTAGGCTCTAAATAT+TGG	-	chr2.1:34165341-34165360	MS.gene071361:CDS	30.0%
!!	TGTTCTAGGCTCTAAATATT+GGG	-	chr2.1:34165342-34165361	MS.gene071361:CDS	30.0%
	AGTTGACCTGTGATAAATCA+AGG	-	chr2.1:34165296-34165315	MS.gene071361:CDS	35.0%
	ATGGACAACTGATGAAAAAG+TGG	-	chr2.1:34165136-34165155	MS.gene071361:CDS	35.0%
	ATTCTGTGAGTTGTCTTACT+TGG	-	chr2.1:34165411-34165430	MS.gene071361:CDS	35.0%
	ATTTATCACAGGTCAACTTG+AGG	+	chr2.1:34165294-34165313	None:intergenic	35.0%
	CATCAAGGTCAGAAAGATTA+AGG	+	chr2.1:34165106-34165125	None:intergenic	35.0%
	CTCTAAATATTGGGGTAAAG+GGG	-	chr2.1:34165351-34165370	MS.gene071361:CDS	35.0%
	TCAAGGTCAGAAAGATTAAG+GGG	+	chr2.1:34165104-34165123	None:intergenic	35.0%
	TGTTGTGAAACAAGTGGTTA+AGG	-	chr2.1:34165384-34165403	MS.gene071361:CDS	35.0%
!	GCTCTAAATATTGGGGTAAA+GGG	-	chr2.1:34165350-34165369	MS.gene071361:CDS	35.0%
!!	GTTCTAGGCTCTAAATATTG+GGG	-	chr2.1:34165343-34165362	MS.gene071361:CDS	35.0%
	AGGAACAAATGTGCAGAACT+TGG	-	chr2.1:34165316-34165335	MS.gene071361:CDS	40.0%
	ATTCTCTCTTCTTTGTCACG+TGG	+	chr2.1:34165056-34165075	None:intergenic	40.0%
	GCATCAATGATCGTGCAATT+GGG	-	chr2.1:34165255-34165274	MS.gene071361:intron	40.0%
	GTGAGTTGTCTTACTTGGAA+AGG	-	chr2.1:34165416-34165435	MS.gene071361:CDS	40.0%
	TCCATACCATGAGATCATCA+AGG	+	chr2.1:34165121-34165140	None:intergenic	40.0%
	TGCATCAATGATCGTGCAAT+TGG	-	chr2.1:34165254-34165273	MS.gene071361:intron	40.0%
!	ACCTTGATGATCTCATGGTA+TGG	-	chr2.1:34165117-34165136	MS.gene071361:CDS	40.0%
!	GCTTGAAGCTTTAGTTGATG+TGG	-	chr2.1:34165438-34165457	MS.gene071361:CDS	40.0%
!	TGTTGGGTCTCAAAGAGTTT+TGG	-	chr2.1:34165465-34165484	MS.gene071361:CDS	40.0%
!	TTCTGACCTTGATGATCTCA+TGG	-	chr2.1:34165112-34165131	MS.gene071361:CDS	40.0%
!!	GGCTCTAAATATTGGGGTAA+AGG	-	chr2.1:34165349-34165368	MS.gene071361:CDS	40.0%
!!!	CAAAGAGTTTTGGAGAAAGC+TGG	-	chr2.1:34165475-34165494	MS.gene071361:CDS	40.0%
	CACGTGTGTTGTGAAACAAG+TGG	-	chr2.1:34165378-34165397	MS.gene071361:CDS	45.0%
	CACTTGTTTCACAACACACG+TGG	+	chr2.1:34165380-34165399	None:intergenic	45.0%
	CTCTTCTTTGTCACGTGGTT+TGG	+	chr2.1:34165051-34165070	None:intergenic	45.0%
	GCAGAACTTGGCTATGTTCT+AGG	-	chr2.1:34165328-34165347	MS.gene071361:CDS	45.0%
	GGTCAGAAAGATTAAGGGGT+CGG	+	chr2.1:34165100-34165119	None:intergenic	45.0%
	AAGGGGTCGGAGTGTGATTT+GGG	+	chr2.1:34165087-34165106	None:intergenic	50.0%
	AAGTGGCCAAGTTCTGCTCT+TGG	-	chr2.1:34165153-34165172	MS.gene071361:CDS	50.0%
	AGTGGCCAAGTTCTGCTCTT+GGG	-	chr2.1:34165154-34165173	MS.gene071361:CDS	50.0%
	GAGTTCCCAAGAGCAGAACT+TGG	+	chr2.1:34165162-34165181	None:intergenic	50.0%
	GTTGATGCCATCATCCTTGC+TGG	+	chr2.1:34165189-34165208	None:intergenic	50.0%
	TAAGGGGTCGGAGTGTGATT+TGG	+	chr2.1:34165088-34165107	None:intergenic	50.0%
	CTCTACGCCAGCAAGGATGA+TGG	-	chr2.1:34165179-34165198	MS.gene071361:CDS	55.0%
	TTGGGAACTCTACGCCAGCA+AGG	-	chr2.1:34165172-34165191	MS.gene071361:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	34165026	34165507	34165026	ID=MS.gene071361
chr2.1	mRNA	34165026	34165507	34165026	ID=MS.gene071361.t1;Parent=MS.gene071361
chr2.1	exon	34165257	34165507	34165257	ID=MS.gene071361.t1.exon1;Parent=MS.gene071361.t1
chr2.1	CDS	34165257	34165507	34165257	ID=cds.MS.gene071361.t1;Parent=MS.gene071361.t1
chr2.1	exon	34165026	34165215	34165026	ID=MS.gene071361.t1.exon2;Parent=MS.gene071361.t1
chr2.1	CDS	34165026	34165215	34165026	ID=cds.MS.gene071361.t1;Parent=MS.gene071361.t1

Gene Sequence

>MS.gene071361

ATGGAGATAACTTCTATTTCTTCCAAACCACGTGACAAAGAAGAGAGAATTGATCTAACCCAAATCACACTCCGACCCCTTAATCTTTCTGACCTTGATGATCTCATGGTATGGACAACTGATGAAAAAGTGGCCAAGTTCTGCTCTTGGGAACTCTACGCCAGCAAGGATGATGGCATCAACTTCATTGAAAATATAGCTACTAAGTTTCTATGGTGCAAAGCAATATGCATCAATGATCGTGCAATTGGGTATGTTTCTCTATCCTCAAGTTGACCTGTGATAAATCAAGGAACAAATGTGCAGAACTTGGCTATGTTCTAGGCTCTAAATATTGGGGTAAAGGGGTTGCCACGTGTGTTGTGAAACAAGTGGTTAAGGTTGCATTCTGTGAGTTGTCTTACTTGGAAAGGCTTGAAGCTTTAGTTGATGTGGAAAATGTTGGGTCTCAAAGAGTTTTGGAGAAAGCTGGTTTCCAATAA

Protein sequence

>MS.gene071361.t1

MEITSISSKPRDKEERIDLTQITLRPLNLSDLDDLMVWTTDEKVAKFCSWELYASKDDGINFIENIATKFLWCKAICINDRAIGNKCAELGYVLGSKYWGKGVATCVVKQVVKVAFCELSYLERLEALVDVENVGSQRVLEKAGFQ