AlfalfaGEDB | MS.gene49468

M. sativa cultivar XinJiangDaYe / MS.gene49468

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49468.t1	XP_024639963.1	92.6	190	14	0	1	190	1	190	2.30E-95	358.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49468.t1	P05332	31.8	173	107	3	21	187	8	175	4.1e-16	86.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49468.t1	G7K3P8	92.6	190	14	0	1	190	1719	1908	1.7e-95	358.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene49468.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049567	MS.gene49468	0.820066	8.35E-53	-1.69E-46
MS.gene049669	MS.gene49468	0.809064	2.28E-50	-1.69E-46
MS.gene050780	MS.gene49468	0.841622	4.25E-58	-1.69E-46
MS.gene051492	MS.gene49468	0.851552	8.24E-61	-1.69E-46
MS.gene051744	MS.gene49468	0.835231	1.89E-56	-1.69E-46
MS.gene052586	MS.gene49468	0.80425	2.36E-49	-1.69E-46
MS.gene052867	MS.gene49468	0.808915	2.45E-50	-1.69E-46
MS.gene053094	MS.gene49468	0.81604	6.79E-52	-1.69E-46
MS.gene053641	MS.gene49468	0.815404	9.41E-52	-1.69E-46
MS.gene053643	MS.gene49468	0.824109	9.65E-54	-1.69E-46
MS.gene05414	MS.gene49468	0.831387	1.72E-55	-1.69E-46
MS.gene05415	MS.gene49468	0.809452	1.88E-50	-1.69E-46
MS.gene054236	MS.gene49468	0.82258	2.20E-53	-1.69E-46
MS.gene054576	MS.gene49468	0.826748	2.29E-54	-1.69E-46
MS.gene05468	MS.gene49468	0.821967	3.05E-53	-1.69E-46
MS.gene054775	MS.gene49468	0.809444	1.89E-50	-1.69E-46
MS.gene05510	MS.gene49468	0.800135	1.66E-48	-1.69E-46
MS.gene055701	MS.gene49468	0.801244	9.87E-49	-1.69E-46
MS.gene055778	MS.gene49468	0.800844	1.19E-48	-1.69E-46
MS.gene055818	MS.gene49468	0.813247	2.82E-51	-1.69E-46
MS.gene056366	MS.gene49468	0.801302	9.60E-49	-1.69E-46
MS.gene057026	MS.gene49468	0.800463	1.42E-48	-1.69E-46
MS.gene058477	MS.gene49468	0.830632	2.63E-55	-1.69E-46
MS.gene060998	MS.gene49468	0.802464	5.54E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene49468	MS.gene21827	PPI
MS.gene49468	MS.gene070944	PPI
MS.gene001033	MS.gene49468	PPI
MS.gene49468	MS.gene49470	PPI
MS.gene49468	MS.gene77154	PPI
MS.gene49468	MS.gene024859	PPI
MS.gene49468	MS.gene74082	PPI
MS.gene34370	MS.gene49468	PPI
MS.gene49468	MS.gene49471	PPI
MS.gene49468	MS.gene024857	PPI
MS.gene49468	MS.gene006418	PPI
MS.gene49472	MS.gene49468	PPI
MS.gene49468	MS.gene95801	PPI
MS.gene77154	MS.gene49468	PPI
MS.gene49468	MS.gene001037	PPI
MS.gene49468	MS.gene21833	PPI
MS.gene49468	MS.gene071357	PPI
MS.gene49468	MS.gene21828	PPI
MS.gene49468	MS.gene001033	PPI
MS.gene49468	MS.gene95802	PPI
MS.gene21833	MS.gene49468	PPI
MS.gene49468	MS.gene024854	PPI
MS.gene043815	MS.gene49468	PPI
MS.gene49468	MS.gene001032	PPI
MS.gene95802	MS.gene49468	PPI
MS.gene49468	MS.gene04530	PPI
MS.gene024858	MS.gene49468	PPI
MS.gene49468	MS.gene024614	PPI
MS.gene49468	MS.gene024612	PPI
MS.gene024855	MS.gene49468	PPI
MS.gene49468	MS.gene001038	PPI
MS.gene49468	MS.gene21830	PPI
MS.gene49468	MS.gene050493	PPI
MS.gene49468	MS.gene001034	PPI
MS.gene001038	MS.gene49468	PPI
MS.gene49468	MS.gene070945	PPI
MS.gene21830	MS.gene49468	PPI
MS.gene49468	MS.gene22888	PPI
MS.gene49468	MS.gene071361	PPI
MS.gene29937	MS.gene49468	PPI
MS.gene49468	MS.gene043813	PPI
MS.gene49468	MS.gene21831	PPI
MS.gene006418	MS.gene49468	PPI
MS.gene49468	MS.gene34371	PPI
MS.gene49468	MS.gene006417	PPI
MS.gene024854	MS.gene49468	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49468.t1	MTR_5g006520	92.632	190	14	0	1	190	1719	1908	1.55e-121	378
MS.gene49468.t1	MTR_5g006450	78.421	190	41	0	1	190	1	190	1.49e-107	306
MS.gene49468.t1	MTR_5g006470	75.789	190	44	1	1	190	1	188	3.98e-103	295
MS.gene49468.t1	MTR_5g006460	70.256	195	51	5	1	190	1	193	7.91e-91	264
MS.gene49468.t1	MTR_5g006510	67.539	191	55	3	1	190	7	191	4.71e-87	254
MS.gene49468.t1	MTR_1g103110	61.458	192	70	2	1	190	1	190	5.91e-83	244
MS.gene49468.t1	MTR_1g103150	59.375	192	74	2	1	190	1	190	6.23e-80	236
MS.gene49468.t1	MTR_7g114410	42.105	171	96	1	17	187	4	171	2.76e-47	153
MS.gene49468.t1	MTR_3g463610	38.953	172	105	0	16	187	2	173	9.44e-45	147
MS.gene49468.t1	MTR_7g114410	41.667	156	88	1	17	172	4	156	3.30e-42	139
MS.gene49468.t1	MTR_7g024760	41.071	168	94	3	20	187	7	169	2.35e-38	130
MS.gene49468.t1	MTR_7g024780	39.080	174	96	3	17	187	4	170	3.37e-37	127
MS.gene49468.t1	MTR_7g024765	39.881	168	96	3	20	187	7	169	1.82e-35	122
MS.gene49468.t1	MTR_1g103320	55.556	81	34	1	51	131	11	89	6.23e-22	86.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49468.t1	AT2G32030	51.190	168	75	3	21	187	24	185	2.45e-58	181
MS.gene49468.t1	AT2G32020	46.561	189	84	4	2	187	6	180	3.15e-54	171
MS.gene49468.t1	AT3G22560	40.237	169	96	2	21	187	7	172	3.01e-37	127

Find 38 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCTAAGAATTCCTTCCTTC+TGG	0.242611	5.1:+1264138	None:intergenic
ACAGAACTTGCCTATGTTCT+AGG	0.250597	5.1:-1264311	MS.gene49468:CDS
GTTGATGCCTTGTTCTTTGC+TGG	0.260002	5.1:+1264454	None:intergenic
ATGTTCTAGGATCTAAATAT+TGG	0.302360	5.1:-1264298	MS.gene49468:CDS
CCTTGTTCTTTGCTGGTGTA+TGG	0.318147	5.1:+1264461	None:intergenic
TGTTCTAGGATCTAAATATT+GGG	0.318462	5.1:-1264297	MS.gene49468:CDS
TGCCTTAATGATCGTGCTAT+TGG	0.357294	5.1:-1264386	MS.gene49468:CDS
ATATACCAAACAAATTTCTA+TGG	0.388477	5.1:-1264421	MS.gene49468:CDS
TCCAGAAGGAAGGAATTCTT+AGG	0.397920	5.1:-1264139	MS.gene49468:CDS
GGCCAATAGCACGATCATTA+AGG	0.403869	5.1:+1264384	None:intergenic
TTGCACCATAGAAATTTGTT+TGG	0.404833	5.1:+1264416	None:intergenic
TTGTTGATGTTGAAAATGTT+GGG	0.408191	5.1:-1264190	MS.gene49468:CDS
GAAAAGGTCGGAGTGCGATT+TGG	0.410859	5.1:+1264555	None:intergenic
CCAAACAAATAAATCATAGT+TGG	0.419913	5.1:+1264051	None:intergenic
TTCTGACCTTGATGAGCTCC+TGG	0.433077	5.1:-1264528	MS.gene49468:CDS
TTTGTTGATGTTGAAAATGT+TGG	0.442167	5.1:-1264191	MS.gene49468:CDS
AGTGGCCAAGTTCTGCTCTT+GGG	0.462852	5.1:-1264486	MS.gene49468:CDS
AAGTGGCCAAGTTCTGCTCT+TGG	0.474319	5.1:-1264487	MS.gene49468:CDS
GGATCTAAATATTGGGGTAA+AGG	0.475846	5.1:-1264290	MS.gene49468:CDS
AGGAAGTATTTGTTCATGAA+AGG	0.475944	5.1:-1264119	MS.gene49468:CDS
TTTCATCAGCTCTCCATACC+AGG	0.488476	5.1:+1264510	None:intergenic
CAAAGAGTGTTGGAGAAAGC+TGG	0.497277	5.1:-1264164	MS.gene49468:CDS
TCAAGGTCAGAAAGATGAAA+AGG	0.513299	5.1:+1264539	None:intergenic
ATATTTAGATCCTAGAACAT+AGG	0.519203	5.1:+1264301	None:intergenic
ACCTTGATGAGCTCCTGGTA+TGG	0.539184	5.1:-1264523	MS.gene49468:CDS
GATCACCTCATGATAAAAGC+AGG	0.555612	5.1:-1264343	MS.gene49468:CDS
TCCATACCAGGAGCTCATCA+AGG	0.558283	5.1:+1264522	None:intergenic
TGTTGGGTCTCAAAGAGTGT+TGG	0.566749	5.1:-1264174	MS.gene49468:CDS
TGGTTCCCAAGAGCAGAACT+TGG	0.574822	5.1:+1264481	None:intergenic
GTTCTAGGATCTAAATATTG+GGG	0.601221	5.1:-1264296	MS.gene49468:CDS
CCATACACCAGCAAAGAACA+AGG	0.601334	5.1:-1264461	MS.gene49468:CDS
GGAGAAAGCTGGTTTCCAGA+AGG	0.617484	5.1:-1264153	MS.gene49468:CDS
AACTTGTTTCACAACATACG+TGG	0.623050	5.1:+1264262	None:intergenic
AAAGCTGGTTTCCAGAAGGA+AGG	0.623258	5.1:-1264149	MS.gene49468:CDS
GGTCAGAAAGATGAAAAGGT+CGG	0.638104	5.1:+1264543	None:intergenic
TGTTGTGAAACAAGTTGTCA+AGG	0.644645	5.1:-1264255	MS.gene49468:CDS
TTTATTTCTTCCAAACCAGG+TGG	0.661410	5.1:-1264602	MS.gene49468:CDS
ATCTTGATTTCAGAGAAACA+CGG	0.706701	5.1:+1264363	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATACCAAACAAATTTCTA+TGG	-	chr5.1:1264257-1264276	MS.gene49468:CDS	20.0%
!	ATATTTAGATCCTAGAACAT+AGG	+	chr5.1:1264380-1264399	None:intergenic	25.0%
!	TTGTTGATGTTGAAAATGTT+GGG	-	chr5.1:1264488-1264507	MS.gene49468:CDS	25.0%
!	TTTGTTGATGTTGAAAATGT+TGG	-	chr5.1:1264487-1264506	MS.gene49468:CDS	25.0%
!!!	ATGTTCTAGGATCTAAATAT+TGG	-	chr5.1:1264380-1264399	MS.gene49468:CDS	25.0%
!!!	TGTTCTAGGATCTAAATATT+GGG	-	chr5.1:1264381-1264400	MS.gene49468:CDS	25.0%
	ATCTTGATTTCAGAGAAACA+CGG	+	chr5.1:1264318-1264337	None:intergenic	30.0%
!	TTGCACCATAGAAATTTGTT+TGG	+	chr5.1:1264265-1264284	None:intergenic	30.0%
!!	ACTTTTATTTCTTCCAAACC+AGG	-	chr5.1:1264073-1264092	MS.gene49468:CDS	30.0%
!!	AGGAAGTATTTGTTCATGAA+AGG	-	chr5.1:1264559-1264578	MS.gene49468:CDS	30.0%
!!	GTTCTAGGATCTAAATATTG+GGG	-	chr5.1:1264382-1264401	MS.gene49468:CDS	30.0%
	GGATCTAAATATTGGGGTAA+AGG	-	chr5.1:1264388-1264407	MS.gene49468:CDS	35.0%
	TCAAGGTCAGAAAGATGAAA+AGG	+	chr5.1:1264142-1264161	None:intergenic	35.0%
	TGTTGTGAAACAAGTTGTCA+AGG	-	chr5.1:1264423-1264442	MS.gene49468:CDS	35.0%
!	AACTTGTTTCACAACATACG+TGG	+	chr5.1:1264419-1264438	None:intergenic	35.0%
!	TTTATTTCTTCCAAACCAGG+TGG	-	chr5.1:1264076-1264095	MS.gene49468:CDS	35.0%
!!	TTGTTCCTGCTTTTATCATG+AGG	+	chr5.1:1264343-1264362	None:intergenic	35.0%
	ACAGAACTTGCCTATGTTCT+AGG	-	chr5.1:1264367-1264386	MS.gene49468:CDS	40.0%
	ATGGAGAGCTGATGAAAAAG+TGG	-	chr5.1:1264174-1264193	MS.gene49468:CDS	40.0%
	GATCACCTCATGATAAAAGC+AGG	-	chr5.1:1264335-1264354	MS.gene49468:CDS	40.0%
	GGTCAGAAAGATGAAAAGGT+CGG	+	chr5.1:1264138-1264157	None:intergenic	40.0%
	TCCAGAAGGAAGGAATTCTT+AGG	-	chr5.1:1264539-1264558	MS.gene49468:CDS	40.0%
	TCCTAAGAATTCCTTCCTTC+TGG	+	chr5.1:1264543-1264562	None:intergenic	40.0%
	TGCCTTAATGATCGTGCTAT+TGG	-	chr5.1:1264292-1264311	MS.gene49468:CDS	40.0%
	CCATACACCAGCAAAGAACA+AGG	-	chr5.1:1264217-1264236	MS.gene49468:CDS	45.0%
	CCTTGTTCTTTGCTGGTGTA+TGG	+	chr5.1:1264220-1264239	None:intergenic	45.0%
	GGCCAATAGCACGATCATTA+AGG	+	chr5.1:1264297-1264316	None:intergenic	45.0%
	TTTCATCAGCTCTCCATACC+AGG	+	chr5.1:1264171-1264190	None:intergenic	45.0%
!	AAAGCTGGTTTCCAGAAGGA+AGG	-	chr5.1:1264529-1264548	MS.gene49468:CDS	45.0%
!	ATGCTCTTTTCATTGCCACC+TGG	+	chr5.1:1264094-1264113	None:intergenic	45.0%
!	CTTTTCATTGCCACCTGGTT+TGG	+	chr5.1:1264089-1264108	None:intergenic	45.0%
!	GTTGATGCCTTGTTCTTTGC+TGG	+	chr5.1:1264227-1264246	None:intergenic	45.0%
!	TGTTGGGTCTCAAAGAGTGT+TGG	-	chr5.1:1264504-1264523	MS.gene49468:CDS	45.0%
!!	CAAAGAGTGTTGGAGAAAGC+TGG	-	chr5.1:1264514-1264533	MS.gene49468:CDS	45.0%
	AAGTGGCCAAGTTCTGCTCT+TGG	-	chr5.1:1264191-1264210	MS.gene49468:CDS	50.0%
	ACCTTGATGAGCTCCTGGTA+TGG	-	chr5.1:1264155-1264174	MS.gene49468:CDS	50.0%
	AGTGGCCAAGTTCTGCTCTT+GGG	-	chr5.1:1264192-1264211	MS.gene49468:CDS	50.0%
	GAAAAGGTCGGAGTGCGATT+TGG	+	chr5.1:1264126-1264145	None:intergenic	50.0%
	TCCATACCAGGAGCTCATCA+AGG	+	chr5.1:1264159-1264178	None:intergenic	50.0%
	TGGTTCCCAAGAGCAGAACT+TGG	+	chr5.1:1264200-1264219	None:intergenic	50.0%
	TTCTGACCTTGATGAGCTCC+TGG	-	chr5.1:1264150-1264169	MS.gene49468:CDS	50.0%
!	GGAGAAAGCTGGTTTCCAGA+AGG	-	chr5.1:1264525-1264544	MS.gene49468:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	1264064	1264636	1264064	ID=MS.gene49468
chr5.1	mRNA	1264064	1264636	1264064	ID=MS.gene49468.t1;Parent=MS.gene49468
chr5.1	exon	1264064	1264636	1264064	ID=MS.gene49468.t1.exon1;Parent=MS.gene49468.t1
chr5.1	CDS	1264064	1264636	1264064	ID=cds.MS.gene49468.t1;Parent=MS.gene49468.t1

Gene Sequence

>MS.gene49468

ATGGAGGAAACTTTTATTTCTTCCAAACCAGGTGGCAATGAAAAGAGCATTGATTTGAACCAAATCGCACTCCGACCTTTTCATCTTTCTGACCTTGATGAGCTCCTGGTATGGAGAGCTGATGAAAAAGTGGCCAAGTTCTGCTCTTGGGAACCATACACCAGCAAAGAACAAGGCATCAACTTCATTGAAAATATACCAAACAAATTTCTATGGTGCAAAGCAATATGCCTTAATGATCGTGCTATTGGCCGTGTTTCTCTGAAATCAAGATCACCTCATGATAAAAGCAGGAACAAAACTACAGAACTTGCCTATGTTCTAGGATCTAAATATTGGGGTAAAGGAATTGCCACGTATGTTGTGAAACAAGTTGTCAAGGTTGCATTCAGTGAGTTGTCACACTTAGAAAGAGTTGAAGCTTTTGTTGATGTTGAAAATGTTGGGTCTCAAAGAGTGTTGGAGAAAGCTGGTTTCCAGAAGGAAGGAATTCTTAGGAAGTATTTGTTCATGAAAGGAAAAAGTAGAGATATGATCATATTCAGTGTTCTTTCTAATGATCTCCAACTATGA

Protein sequence

>MS.gene49468.t1

MEETFISSKPGGNEKSIDLNQIALRPFHLSDLDELLVWRADEKVAKFCSWEPYTSKEQGINFIENIPNKFLWCKAICLNDRAIGRVSLKSRSPHDKSRNKTTELAYVLGSKYWGKGIATYVVKQVVKVAFSELSHLERVEAFVDVENVGSQRVLEKAGFQKEGILRKYLFMKGKSRDMIIFSVLSNDLQL