AlfalfaGEDB | MS.gene95802

M. sativa cultivar XinJiangDaYe / MS.gene95802

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95802.t1	XP_013469780.1	73.1	182	10	2	2	144	1	182	2.70E-61	245

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95802.t1	P05332	34.2	149	82	4	16	150	30	176	6.3e-11	68.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95802.t1	A0A072VQW9	73.1	182	10	2	2	144	1	182	1.9e-61	245.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene001038	MS.gene95802	PPI
MS.gene95802	MS.gene21830	PPI
MS.gene21830	MS.gene95802	PPI
MS.gene95802	MS.gene001034	PPI
MS.gene95802	MS.gene070945	PPI
MS.gene29937	MS.gene95802	PPI
MS.gene95802	MS.gene22888	PPI
MS.gene95802	MS.gene071361	PPI
MS.gene95802	MS.gene043813	PPI
MS.gene006418	MS.gene95802	PPI
MS.gene95802	MS.gene21831	PPI
MS.gene95802	MS.gene77154	PPI
MS.gene024854	MS.gene95802	PPI
MS.gene95802	MS.gene34371	PPI
MS.gene95802	MS.gene006417	PPI
MS.gene95802	MS.gene21827	PPI
MS.gene95802	MS.gene070944	PPI
MS.gene95802	MS.gene95801	PPI
MS.gene001033	MS.gene95802	PPI
MS.gene49468	MS.gene95802	PPI
MS.gene95802	MS.gene49470	PPI
MS.gene95802	MS.gene49468	PPI
MS.gene95802	MS.gene024859	PPI
MS.gene34370	MS.gene95802	PPI
MS.gene95802	MS.gene001032	PPI
MS.gene49472	MS.gene95802	PPI
MS.gene95802	MS.gene49471	PPI
MS.gene95802	MS.gene024857	PPI
MS.gene95802	MS.gene024612	PPI
MS.gene95802	MS.gene006418	PPI
MS.gene77154	MS.gene95802	PPI
MS.gene95802	MS.gene001037	PPI
MS.gene95802	MS.gene21833	PPI
MS.gene95802	MS.gene001038	PPI
MS.gene95802	MS.gene071357	PPI
MS.gene95802	MS.gene050493	PPI
MS.gene95802	MS.gene21828	PPI
MS.gene21833	MS.gene95802	PPI
MS.gene043815	MS.gene95802	PPI
MS.gene95802	MS.gene001033	PPI
MS.gene95802	MS.gene024854	PPI
MS.gene024858	MS.gene95802	PPI
MS.gene95802	MS.gene04530	PPI
MS.gene95802	MS.gene74082	PPI
MS.gene024855	MS.gene95802	PPI
MS.gene95802	MS.gene024614	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95802.t1	MTR_1g103110	73.077	182	10	2	2	144	1	182	5.47e-86	251
MS.gene95802.t1	MTR_1g103150	67.582	182	20	2	2	144	1	182	5.94e-81	238
MS.gene95802.t1	MTR_5g006470	64.748	139	40	1	15	144	42	180	1.22e-60	186
MS.gene95802.t1	MTR_5g006450	63.121	141	41	2	15	144	42	182	4.72e-58	180
MS.gene95802.t1	MTR_5g006520	60.284	141	45	2	15	144	1760	1900	5.42e-51	175
MS.gene95802.t1	MTR_5g006510	56.338	142	48	4	15	144	44	183	1.08e-47	154
MS.gene95802.t1	MTR_5g006460	58.621	145	43	5	15	144	43	185	1.20e-47	154
MS.gene95802.t1	MTR_7g114410	41.259	143	70	3	13	144	27	166	1.46e-29	107
MS.gene95802.t1	MTR_3g463610	38.356	146	73	3	13	144	26	168	5.19e-29	107
MS.gene95802.t1	MTR_1g103320	55.340	103	36	2	24	116	11	113	1.98e-28	102
MS.gene95802.t1	MTR_7g114410	41.353	133	64	3	13	134	27	156	1.71e-26	99.0
MS.gene95802.t1	MTR_7g024760	36.879	141	77	4	13	144	27	164	4.41e-21	85.5
MS.gene95802.t1	MTR_7g024780	37.692	130	70	3	24	144	38	165	4.70e-21	85.1
MS.gene95802.t1	MTR_7g024765	36.170	141	78	4	13	144	27	164	3.09e-20	83.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95802.t1	AT2G32030	48.551	138	57	4	17	144	47	180	9.58e-40	133
MS.gene95802.t1	AT2G32020	49.640	139	56	4	16	144	41	175	1.72e-39	132
MS.gene95802.t1	AT3G22560	40.411	146	71	4	15	147	28	170	4.58e-25	95.9

Find 30 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTTCTTTAGCATCTGATT+TGG	0.255230	1.1:+74004806	None:intergenic
CCGTCTTCTTTGTTTGTATA+AGG	0.260514	1.1:+74004668	None:intergenic
TGCTATGAGCTCCAAGTATT+GGG	0.261207	1.1:-74004510	MS.gene95802:CDS
GAGATTTGCTTGCTATGTTT+TGG	0.329625	1.1:+74004633	None:intergenic
TTTAGCATCTGATTTGGAAT+AGG	0.367598	1.1:+74004812	None:intergenic
AATACCTTTGCCCCAATACT+TGG	0.403153	1.1:+74004499	None:intergenic
TGCCTTCGCGATCGAGCAAT+CGG	0.411073	1.1:-74004593	MS.gene95802:CDS
CAGAGGGTGTTGGAGAAGGC+TGG	0.411839	1.1:-74004323	MS.gene95802:CDS
ACATAGCAAGCAAATCTCTA+TGG	0.412043	1.1:-74004628	MS.gene95802:CDS
AGGAACAAATCTGCTGAACT+TGG	0.442081	1.1:-74004536	MS.gene95802:CDS
ATGCTATGAGCTCCAAGTAT+TGG	0.448265	1.1:-74004511	MS.gene95802:CDS
CTTGAGCTTCAAGCCGCTCC+AGG	0.458231	1.1:+74004369	None:intergenic
TTCTCAGAGGGTGTTGGAGA+AGG	0.470170	1.1:-74004327	MS.gene95802:CDS
CAGAAAATATCTGTTCATCA+AGG	0.472630	1.1:-74004279	MS.gene95802:CDS
GCTTCAAGCCGCTCCAGGTA+TGG	0.487071	1.1:+74004374	None:intergenic
AGGTTCCCAAGTGCAATACT+TGG	0.527166	1.1:+74004688	None:intergenic
AGCTCCAAGTATTGGGGCAA+AGG	0.537498	1.1:-74004503	MS.gene95802:intron
TAGAAAATGTGGCTTCTCAG+AGG	0.540854	1.1:-74004340	MS.gene95802:CDS
ATTTAAAGAGTTTCCATACC+TGG	0.542410	1.1:-74004387	MS.gene95802:CDS
GCCTTCGCGATCGAGCAATC+GGG	0.544699	1.1:-74004592	MS.gene95802:CDS
TGTGGCTTCTCAGAGGGTGT+TGG	0.549890	1.1:-74004333	MS.gene95802:CDS
AGAAAATATCTGTTCATCAA+GGG	0.554840	1.1:-74004278	MS.gene95802:CDS
TCAAGTTGATGTAGAAAATG+TGG	0.572167	1.1:-74004351	MS.gene95802:CDS
AAGAGTTTCCATACCTGGAG+CGG	0.585589	1.1:-74004382	MS.gene95802:CDS
AAGTGGCCAAGTATTGCACT+TGG	0.601796	1.1:-74004694	MS.gene95802:CDS
AGAAAATGTGGCTTCTCAGA+GGG	0.613906	1.1:-74004339	MS.gene95802:CDS
CCTTATACAAACAAAGAAGA+CGG	0.623002	1.1:-74004668	MS.gene95802:CDS
ACCCGATTGCTCGATCGCGA+AGG	0.639229	1.1:+74004591	None:intergenic
GCTATGAGCTCCAAGTATTG+GGG	0.656803	1.1:-74004509	MS.gene95802:CDS
AGTGGCCAAGTATTGCACTT+GGG	0.670287	1.1:-74004693	MS.gene95802:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATCAATTTCTTTTTGTGTT+CGG	-	chr1.1:74004415-74004434	MS.gene95802:intron	20.0%
!!!	TTTTAGATCAAATATATCAG+TGG	+	chr1.1:74004801-74004820	None:intergenic	20.0%
!	AGAAAATATCTGTTCATCAA+GGG	-	chr1.1:74004704-74004723	MS.gene95802:intron	25.0%
	ATTTAAAGAGTTTCCATACC+TGG	-	chr1.1:74004595-74004614	MS.gene95802:CDS	30.0%
	CAAGATCTGAAAGACTAATT+GGG	+	chr1.1:74004238-74004257	None:intergenic	30.0%
	CAGAAAATATCTGTTCATCA+AGG	-	chr1.1:74004703-74004722	MS.gene95802:intron	30.0%
	CCTTATACAAACAAAGAAGA+CGG	-	chr1.1:74004314-74004333	MS.gene95802:CDS	30.0%
	TCAAGATCTGAAAGACTAAT+TGG	+	chr1.1:74004239-74004258	None:intergenic	30.0%
!!	TCAAGTTGATGTAGAAAATG+TGG	-	chr1.1:74004631-74004650	MS.gene95802:CDS	30.0%
!!	TTTAGCATCTGATTTGGAAT+AGG	+	chr1.1:74004173-74004192	None:intergenic	30.0%
!!!	TTCAGATCTTGATGATGTTT+TGG	-	chr1.1:74004247-74004266	MS.gene95802:CDS	30.0%
	AATTGGGTCGAAGAGATATT+TGG	+	chr1.1:74004222-74004241	None:intergenic	35.0%
	ACATAGCAAGCAAATCTCTA+TGG	-	chr1.1:74004354-74004373	MS.gene95802:CDS	35.0%
	CAAGGGAAAAAGTAGAGATA+TGG	-	chr1.1:74004721-74004740	MS.gene95802:intron	35.0%
	CTCTTCTTTAGCATCTGATT+TGG	+	chr1.1:74004179-74004198	None:intergenic	35.0%
!	CCGTCTTCTTTGTTTGTATA+AGG	+	chr1.1:74004317-74004336	None:intergenic	35.0%
!!	ACCACTTTTGACAAAAAGTG+TGG	+	chr1.1:74004566-74004585	None:intergenic	35.0%
!!!	ATCTTGATGATGTTTTGGTG+TGG	-	chr1.1:74004252-74004271	MS.gene95802:CDS	35.0%
!!!	GAGATTTGCTTGCTATGTTT+TGG	+	chr1.1:74004352-74004371	None:intergenic	35.0%
	AATACCTTTGCCCCAATACT+TGG	+	chr1.1:74004486-74004505	None:intergenic	40.0%
	AGAAAATGTGGCTTCTCAGA+GGG	-	chr1.1:74004643-74004662	MS.gene95802:CDS	40.0%
	AGGAACAAATCTGCTGAACT+TGG	-	chr1.1:74004446-74004465	MS.gene95802:intron	40.0%
	ATGCTATGAGCTCCAAGTAT+TGG	-	chr1.1:74004471-74004490	MS.gene95802:intron	40.0%
	GTGGACAAGTGATGAAAAAG+TGG	-	chr1.1:74004271-74004290	MS.gene95802:CDS	40.0%
	TAGAAAATGTGGCTTCTCAG+AGG	-	chr1.1:74004642-74004661	MS.gene95802:CDS	40.0%
	TGCTATGAGCTCCAAGTATT+GGG	-	chr1.1:74004472-74004491	MS.gene95802:intron	40.0%
!	TTTTGTGTTCGGATAGATGC+AGG	-	chr1.1:74004426-74004445	MS.gene95802:intron	40.0%
!!	GCCACACTTTTTGTCAAAAG+TGG	-	chr1.1:74004562-74004581	MS.gene95802:CDS	40.0%
	AAGAGTTTCCATACCTGGAG+CGG	-	chr1.1:74004600-74004619	MS.gene95802:CDS	45.0%
	AGGTTCCCAAGTGCAATACT+TGG	+	chr1.1:74004297-74004316	None:intergenic	45.0%
	TATTGCCACACTGCTGAACT+TGG	-	chr1.1:74004502-74004521	MS.gene95802:intron	45.0%
!	AAGTGGCCAAGTATTGCACT+TGG	-	chr1.1:74004288-74004307	MS.gene95802:CDS	45.0%
!	AGTGGCCAAGTATTGCACTT+GGG	-	chr1.1:74004289-74004308	MS.gene95802:CDS	45.0%
!	GCTATGAGCTCCAAGTATTG+GGG	-	chr1.1:74004473-74004492	MS.gene95802:intron	45.0%
!!!	AAGGCTGGTTTTCAAAGGGA+GGG	-	chr1.1:74004674-74004693	MS.gene95802:CDS	45.0%
!!!	GGAGAAGGCTGGTTTTCAAA+GGG	-	chr1.1:74004670-74004689	MS.gene95802:CDS	45.0%
!!!	TGGAGAAGGCTGGTTTTCAA+AGG	-	chr1.1:74004669-74004688	MS.gene95802:CDS	45.0%
	CATAGCCAAGTTCAGCAGTG+TGG	+	chr1.1:74004510-74004529	None:intergenic	50.0%
!!	AGCTCCAAGTATTGGGGCAA+AGG	-	chr1.1:74004479-74004498	MS.gene95802:intron	50.0%
!!	TTCTCAGAGGGTGTTGGAGA+AGG	-	chr1.1:74004655-74004674	MS.gene95802:CDS	50.0%
!!!	GAAGGCTGGTTTTCAAAGGG+AGG	-	chr1.1:74004673-74004692	MS.gene95802:CDS	50.0%
	TGCCTTCGCGATCGAGCAAT+CGG	-	chr1.1:74004389-74004408	MS.gene95802:CDS	55.0%
!	TGTGGCTTCTCAGAGGGTGT+TGG	-	chr1.1:74004649-74004668	MS.gene95802:CDS	55.0%
	ACCCGATTGCTCGATCGCGA+AGG	+	chr1.1:74004394-74004413	None:intergenic	60.0%
	CTTGAGCTTCAAGCCGCTCC+AGG	+	chr1.1:74004616-74004635	None:intergenic	60.0%
	GCCTTCGCGATCGAGCAATC+GGG	-	chr1.1:74004390-74004409	MS.gene95802:CDS	60.0%
	GCTTCAAGCCGCTCCAGGTA+TGG	+	chr1.1:74004611-74004630	None:intergenic	60.0%
!!	CAGAGGGTGTTGGAGAAGGC+TGG	-	chr1.1:74004659-74004678	MS.gene95802:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	74004151	74004853	74004151	ID=MS.gene95802
chr1.1	mRNA	74004151	74004853	74004151	ID=MS.gene95802.t1;Parent=MS.gene95802
chr1.1	exon	74004805	74004853	74004805	ID=MS.gene95802.t1.exon1;Parent=MS.gene95802.t1
chr1.1	CDS	74004805	74004853	74004805	ID=cds.MS.gene95802.t1;Parent=MS.gene95802.t1
chr1.1	exon	74004504	74004713	74004504	ID=MS.gene95802.t1.exon2;Parent=MS.gene95802.t1
chr1.1	CDS	74004504	74004713	74004504	ID=cds.MS.gene95802.t1;Parent=MS.gene95802.t1
chr1.1	exon	74004151	74004422	74004151	ID=MS.gene95802.t1.exon3;Parent=MS.gene95802.t1
chr1.1	CDS	74004151	74004422	74004151	ID=cds.MS.gene95802.t1;Parent=MS.gene95802.t1

Gene Sequence

>MS.gene95802

ATGATGGAAAAAACTGCTACCTATTCCAAATCAGATGCTAAAGAAGAGTGTTCTGTTGTTGACTTGAGCCAAATATCTCTTCGACCCAATTAGTCTTTCAGATCTTGATGATGTTTTGGTGTGGACAAGTGATGAAAAAGTGGCCAAGTATTGCACTTGGGAACCTTATACAAACAAAGAAGACGGCATTGACTTCATCCAAAACATAGCAAGCAAATCTCTATGGTTCAGAGCAATTTGCCTTCGCGATCGAGCAATCGGGTGTATCAATTTCTTTTTGTGTTCGGATAGATGCAGGAACAAATCTGCTGAACTTGGCTATGCTATGAGCTCCAAGTATTGGGGCAAAGGTATTGCCACACTGCTGAACTTGGCTATGCTATGAGCTCCAAGTATTGGGGCAAAGGTATTGCCACACTTTTTGTCAAAAGTGGTTGAAGCTGCATTTAAAGAGTTTCCATACCTGGAGCGGCTTGAAGCTCAAGTTGATGTAGAAAATGTGGCTTCTCAGAGGGTGTTGGAGAAGGCTGGTTTTCAAAGGGAGGGTGTTCTCAGAAAATATCTGTTCATCAAGGGAAAAAGTAGAGATATGGTTATGTTCTCTCAAGTGAAGTTCAAATTTGATCTGTTTGTTTTCAAGTATGTGACCACTGATATATTTGATCTAAAACATCTTCAGTATTTTCAAGTATGTGACCACTGA

Protein sequence

>MS.gene95802.t1

MMEKTATYSKSDAKEELAKYCTWEPYTNKEDGIDFIQNIASKSLWFRAICLRDRAIGCINFFLCSDRCRNKSAELGYAMSSKYWGKVVEAAFKEFPYLERLEAQVDVENVASQRVLEKAGFQREGVLRKYLFIKGKSRDMVMFSQVKFKFDLFVFKYVTTDIFDLKHLQYFQVCDH