AlfalfaGEDB | MS.gene024859

M. sativa cultivar XinJiangDaYe / MS.gene024859

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024859.t1	XP_003610737.1	93.7	190	12	0	1	190	1	190	1.30E-93	352.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024859.t1	P05332	31.8	179	111	3	15	187	2	175	8.6e-14	78.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024859.t1	G7K3P2	93.7	190	12	0	1	190	1	190	9.2e-94	352.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene024859.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene024859	MS.gene050493	PPI
MS.gene024859	MS.gene001034	PPI
MS.gene024859	MS.gene070945	PPI
MS.gene001033	MS.gene024859	PPI
MS.gene024859	MS.gene22888	PPI
MS.gene024859	MS.gene04530	PPI
MS.gene49468	MS.gene024859	PPI
MS.gene024859	MS.gene043813	PPI
MS.gene024859	MS.gene024614	PPI
MS.gene024859	MS.gene21831	PPI
MS.gene024859	MS.gene024612	PPI
MS.gene024854	MS.gene024859	PPI
MS.gene024859	MS.gene34371	PPI
MS.gene024859	MS.gene006417	PPI
MS.gene024859	MS.gene21827	PPI
MS.gene024859	MS.gene070944	PPI
MS.gene024859	MS.gene071361	PPI
MS.gene95802	MS.gene024859	PPI
MS.gene34370	MS.gene024859	PPI
MS.gene024859	MS.gene49470	PPI
MS.gene49472	MS.gene024859	PPI
MS.gene77154	MS.gene024859	PPI
MS.gene024859	MS.gene74082	PPI
MS.gene024859	MS.gene024857	PPI
MS.gene001038	MS.gene024859	PPI
MS.gene024859	MS.gene95801	PPI
MS.gene070945	MS.gene024859	PPI
MS.gene21830	MS.gene024859	PPI
MS.gene024859	MS.gene001037	PPI
MS.gene21833	MS.gene024859	PPI
MS.gene043815	MS.gene024859	PPI
MS.gene024859	MS.gene071357	PPI
MS.gene024859	MS.gene21828	PPI
MS.gene29937	MS.gene024859	PPI
MS.gene024858	MS.gene024859	PPI
MS.gene024855	MS.gene024859	PPI
MS.gene006418	MS.gene024859	PPI
MS.gene024859	MS.gene001032	PPI
MS.gene024859	MS.gene49471	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024859.t1	MTR_5g006450	93.684	190	12	0	1	190	1	190	3.14e-129	361
MS.gene024859.t1	MTR_5g006470	76.842	190	42	1	1	190	1	188	1.11e-101	291
MS.gene024859.t1	MTR_5g006520	77.895	190	42	0	1	190	1719	1908	7.35e-98	310
MS.gene024859.t1	MTR_5g006520	52.000	75	13	1	97	171	1655	1706	2.64e-12	65.1
MS.gene024859.t1	MTR_5g006510	73.298	191	44	3	1	190	7	191	1.05e-94	273
MS.gene024859.t1	MTR_5g006460	73.333	195	45	5	1	190	1	193	1.77e-93	270
MS.gene024859.t1	MTR_1g103110	64.583	192	64	2	1	190	1	190	2.20e-85	250
MS.gene024859.t1	MTR_1g103150	60.417	192	72	2	1	190	1	190	2.37e-79	234
MS.gene024859.t1	MTR_7g114410	41.279	172	98	1	16	187	3	171	1.35e-45	148
MS.gene024859.t1	MTR_3g463610	39.766	171	103	0	17	187	3	173	1.64e-44	147
MS.gene024859.t1	MTR_7g114410	40.764	157	90	1	16	172	3	156	1.45e-41	137
MS.gene024859.t1	MTR_7g024760	41.071	168	94	3	20	187	7	169	2.46e-37	127
MS.gene024859.t1	MTR_7g024780	39.181	171	100	2	17	187	4	170	1.18e-36	125
MS.gene024859.t1	MTR_7g024765	38.690	168	98	3	20	187	7	169	1.30e-35	123
MS.gene024859.t1	MTR_1g103320	55.128	78	33	1	53	130	13	88	2.15e-20	82.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024859.t1	AT2G32030	51.786	168	74	3	21	187	24	185	1.60e-58	182
MS.gene024859.t1	AT2G32020	51.190	168	75	2	21	187	19	180	2.37e-54	171
MS.gene024859.t1	AT3G22560	38.235	170	100	2	20	187	6	172	2.44e-35	122

Find 49 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAAATCAATTCTCTCTTCTT+TGG	0.250774	5.3:+1573065	None:intergenic
ATGTTCTAGGCTCTAAATAT+TGG	0.292142	5.3:-1572783	MS.gene024859:CDS
TCCTTAGAACTCCCTCCTTT+TGG	0.297796	5.3:+1572623	None:intergenic
GCAGAACTTGGCTATGTTCT+AGG	0.302641	5.3:-1572796	MS.gene024859:CDS
TGTTCTAGGCTCTAAATATT+GGG	0.316974	5.3:-1572782	MS.gene024859:CDS
GAGTTCCCAAGAGCAGAATT+TGG	0.329740	5.3:+1572966	None:intergenic
GAAGGGCTCGAAGTGTGATT+TGG	0.338927	5.3:+1573040	None:intergenic
GCTCTAAATATTGGGGTAAA+GGG	0.356917	5.3:-1572774	MS.gene024859:CDS
AAGTTCTGCACATTTGTTCC+TGG	0.375848	5.3:+1572810	None:intergenic
CTAAACAATTGAATTATAGT+TGG	0.392086	5.3:+1572536	None:intergenic
ATATAGCTACCAAGTTTCTA+TGG	0.402105	5.3:-1572906	MS.gene024859:CDS
TGCATCAATGATCGTGCAAT+TGG	0.403589	5.3:-1572871	MS.gene024859:CDS
AAGGGCTCGAAGTGTGATTT+GGG	0.403990	5.3:+1573041	None:intergenic
TCCAAAAGGAGGGAGTTCTA+AGG	0.410439	5.3:-1572624	MS.gene024859:CDS
GGCTCTAAATATTGGGGTAA+AGG	0.422886	5.3:-1572775	MS.gene024859:CDS
AAGTGGCCAAATTCTGCTCT+TGG	0.423997	5.3:-1572972	MS.gene024859:CDS
GTTGATGCCATCATCCTTGC+TGG	0.429573	5.3:+1572939	None:intergenic
TGCTGGGTCTCAAAGAGTGC+TGG	0.460731	5.3:-1572659	MS.gene024859:CDS
AGTGGCCAAATTCTGCTCTT+GGG	0.474265	5.3:-1572971	MS.gene024859:CDS
AGGAACAAATGTGCAGAACT+TGG	0.475881	5.3:-1572808	MS.gene024859:CDS
AGGAAATATTTGTTCATGAA+AGG	0.480337	5.3:-1572604	MS.gene024859:CDS
GCTTGAAGCTTTAGTTGATG+TGG	0.488968	5.3:-1572686	MS.gene024859:CDS
TGCTTTGCACCATAGAAACT+TGG	0.496119	5.3:+1572897	None:intergenic
TTCTGACCTTGATGATCTCA+TGG	0.505164	5.3:-1573013	MS.gene024859:CDS
CATCAAGGTCAGAAAGTTGA+AGG	0.505953	5.3:+1573022	None:intergenic
TGTTGTGAAACAAGTTGTTA+AGG	0.509712	5.3:-1572740	MS.gene024859:CDS
TTGTTCCTGGATTTATCACC+AGG	0.514781	5.3:+1572823	None:intergenic
ATTCTGTGAGTTGACTTACT+TGG	0.516104	5.3:-1572713	MS.gene024859:CDS
CAAAGAGTGCTGGAGAAAGC+TGG	0.517457	5.3:-1572649	MS.gene024859:CDS
TTAGTTGATGTGGAAAATGC+TGG	0.527178	5.3:-1572676	MS.gene024859:CDS
GTGAGTTGACTTACTTGGAA+AGG	0.541309	5.3:-1572708	MS.gene024859:CDS
GTTCACCTGGTGATAAATCC+AGG	0.542743	5.3:-1572828	MS.gene024859:CDS
TCTCTATCCTCAAGTTCACC+TGG	0.545696	5.3:-1572841	MS.gene024859:CDS
CTCTACACCAGCAAGGATGA+TGG	0.559987	5.3:-1572946	MS.gene024859:CDS
GAAAGCTGGTTTCCAAAAGG+AGG	0.562242	5.3:-1572635	MS.gene024859:CDS
CTCTAAATATTGGGGTAAAG+GGG	0.564900	5.3:-1572773	MS.gene024859:CDS
GCATCAATGATCGTGCAATT+GGG	0.567462	5.3:-1572870	MS.gene024859:CDS
GGAGAAAGCTGGTTTCCAAA+AGG	0.570344	5.3:-1572638	MS.gene024859:CDS
TTTATCACCAGGTGAACTTG+AGG	0.573291	5.3:+1572834	None:intergenic
TAGTTGATGTGGAAAATGCT+GGG	0.573363	5.3:-1572675	MS.gene024859:CDS
ATTCTCTCTTCTTTGGCACG+TGG	0.591899	5.3:+1573072	None:intergenic
ACCTTGATGATCTCATGGTG+TGG	0.606125	5.3:-1573008	MS.gene024859:CDS
AACTTGTTTCACAACACATG+TGG	0.607173	5.3:+1572747	None:intergenic
GTTCTAGGCTCTAAATATTG+GGG	0.619040	5.3:-1572781	MS.gene024859:CDS
TCCACACCATGAGATCATCA+AGG	0.646997	5.3:+1573007	None:intergenic
ATCAAGGTCAGAAAGTTGAA+GGG	0.653293	5.3:+1573023	None:intergenic
AAAGCTGGTTTCCAAAAGGA+GGG	0.657724	5.3:-1572634	MS.gene024859:CDS
GAGATCAGTAGAAAGAACAC+TGG	0.658551	5.3:+1572558	None:intergenic
TTGGGAACTCTACACCAGCA+AGG	0.679749	5.3:-1572953	MS.gene024859:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGGAAATATTTGTTCATGAA+AGG	-	chr5.3:1573044-1573063	MS.gene024859:CDS	25.0%
!	TAAATCAATTCTCTCTTCTT+TGG	+	chr5.3:1572586-1572605	None:intergenic	25.0%
	ATATAGCTACCAAGTTTCTA+TGG	-	chr5.3:1572742-1572761	MS.gene024859:CDS	30.0%
	TGTTGTGAAACAAGTTGTTA+AGG	-	chr5.3:1572908-1572927	MS.gene024859:CDS	30.0%
!!	ATGTTCTAGGCTCTAAATAT+TGG	-	chr5.3:1572865-1572884	MS.gene024859:CDS	30.0%
!!	TGTTCTAGGCTCTAAATATT+GGG	-	chr5.3:1572866-1572885	MS.gene024859:CDS	30.0%
	ATCAAGGTCAGAAAGTTGAA+GGG	+	chr5.3:1572628-1572647	None:intergenic	35.0%
	CTCTAAATATTGGGGTAAAG+GGG	-	chr5.3:1572875-1572894	MS.gene024859:CDS	35.0%
!	AACTTGTTTCACAACACATG+TGG	+	chr5.3:1572904-1572923	None:intergenic	35.0%
!	ATTCTGTGAGTTGACTTACT+TGG	-	chr5.3:1572935-1572954	MS.gene024859:CDS	35.0%
!	GCTCTAAATATTGGGGTAAA+GGG	-	chr5.3:1572874-1572893	MS.gene024859:CDS	35.0%
!	TAGTTGATGTGGAAAATGCT+GGG	-	chr5.3:1572973-1572992	MS.gene024859:CDS	35.0%
!	TTAGTTGATGTGGAAAATGC+TGG	-	chr5.3:1572972-1572991	MS.gene024859:CDS	35.0%
!!	GTTCTAGGCTCTAAATATTG+GGG	-	chr5.3:1572867-1572886	MS.gene024859:CDS	35.0%
	AAGTTCTGCACATTTGTTCC+TGG	+	chr5.3:1572841-1572860	None:intergenic	40.0%
	AGGAACAAATGTGCAGAACT+TGG	-	chr5.3:1572840-1572859	MS.gene024859:CDS	40.0%
	CATCAAGGTCAGAAAGTTGA+AGG	+	chr5.3:1572629-1572648	None:intergenic	40.0%
	GAGATCAGTAGAAAGAACAC+TGG	+	chr5.3:1573093-1573112	None:intergenic	40.0%
	GCATCAATGATCGTGCAATT+GGG	-	chr5.3:1572778-1572797	MS.gene024859:CDS	40.0%
	GTGGACAACTGATGAAAAAG+TGG	-	chr5.3:1572659-1572678	MS.gene024859:CDS	40.0%
	TGCATCAATGATCGTGCAAT+TGG	-	chr5.3:1572777-1572796	MS.gene024859:CDS	40.0%
	TGCTTTGCACCATAGAAACT+TGG	+	chr5.3:1572754-1572773	None:intergenic	40.0%
	TTGTTCCTGGATTTATCACC+AGG	+	chr5.3:1572828-1572847	None:intergenic	40.0%
	TTTATCACCAGGTGAACTTG+AGG	+	chr5.3:1572817-1572836	None:intergenic	40.0%
!	AAAGCTGGTTTCCAAAAGGA+GGG	-	chr5.3:1573014-1573033	MS.gene024859:CDS	40.0%
!	GCTTGAAGCTTTAGTTGATG+TGG	-	chr5.3:1572962-1572981	MS.gene024859:CDS	40.0%
!	GTGAGTTGACTTACTTGGAA+AGG	-	chr5.3:1572940-1572959	MS.gene024859:CDS	40.0%
!	TTCTGACCTTGATGATCTCA+TGG	-	chr5.3:1572635-1572654	MS.gene024859:CDS	40.0%
!!	GGCTCTAAATATTGGGGTAA+AGG	-	chr5.3:1572873-1572892	MS.gene024859:CDS	40.0%
	AAGGGCTCGAAGTGTGATTT+GGG	+	chr5.3:1572610-1572629	None:intergenic	45.0%
	AAGTGGCCAAATTCTGCTCT+TGG	-	chr5.3:1572676-1572695	MS.gene024859:CDS	45.0%
	AGTGGCCAAATTCTGCTCTT+GGG	-	chr5.3:1572677-1572696	MS.gene024859:CDS	45.0%
	ATTCTCTCTTCTTTGGCACG+TGG	+	chr5.3:1572579-1572598	None:intergenic	45.0%
	GAGTTCCCAAGAGCAGAATT+TGG	+	chr5.3:1572685-1572704	None:intergenic	45.0%
	GCAGAACTTGGCTATGTTCT+AGG	-	chr5.3:1572852-1572871	MS.gene024859:CDS	45.0%
	GTTCACCTGGTGATAAATCC+AGG	-	chr5.3:1572820-1572839	MS.gene024859:CDS	45.0%
	TCCAAAAGGAGGGAGTTCTA+AGG	-	chr5.3:1573024-1573043	MS.gene024859:CDS	45.0%
	TCCACACCATGAGATCATCA+AGG	+	chr5.3:1572644-1572663	None:intergenic	45.0%
	TCTCTATCCTCAAGTTCACC+TGG	-	chr5.3:1572807-1572826	MS.gene024859:CDS	45.0%
!	ACCTTGATGATCTCATGGTG+TGG	-	chr5.3:1572640-1572659	MS.gene024859:CDS	45.0%
!	GAAAGCTGGTTTCCAAAAGG+AGG	-	chr5.3:1573013-1573032	MS.gene024859:CDS	45.0%
!	GGAGAAAGCTGGTTTCCAAA+AGG	-	chr5.3:1573010-1573029	MS.gene024859:CDS	45.0%
!	TCCTTAGAACTCCCTCCTTT+TGG	+	chr5.3:1573028-1573047	None:intergenic	45.0%
	CTCTACACCAGCAAGGATGA+TGG	-	chr5.3:1572702-1572721	MS.gene024859:CDS	50.0%
	GAAGGGCTCGAAGTGTGATT+TGG	+	chr5.3:1572611-1572630	None:intergenic	50.0%
	GTTGATGCCATCATCCTTGC+TGG	+	chr5.3:1572712-1572731	None:intergenic	50.0%
	TTGGGAACTCTACACCAGCA+AGG	-	chr5.3:1572695-1572714	MS.gene024859:CDS	50.0%
!!	CAAAGAGTGCTGGAGAAAGC+TGG	-	chr5.3:1572999-1573018	MS.gene024859:CDS	50.0%
!	TGCTGGGTCTCAAAGAGTGC+TGG	-	chr5.3:1572989-1573008	MS.gene024859:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	1572549	1573121	1572549	ID=MS.gene024859
chr5.3	mRNA	1572549	1573121	1572549	ID=MS.gene024859.t1;Parent=MS.gene024859
chr5.3	exon	1572549	1573121	1572549	ID=MS.gene024859.t1.exon1;Parent=MS.gene024859.t1
chr5.3	CDS	1572549	1573121	1572549	ID=cds.MS.gene024859.t1;Parent=MS.gene024859.t1

Gene Sequence

>MS.gene024859

ATGGAGAGAACTTCTATTTCTTTCGAACCACGTGCCAAAGAAGAGAGAATTGATTTAACCCAAATCACACTTCGAGCCCTTCAACTTTCTGACCTTGATGATCTCATGGTGTGGACAACTGATGAAAAAGTGGCCAAATTCTGCTCTTGGGAACTCTACACCAGCAAGGATGATGGCATCAACTTCATTGAAAATATAGCTACCAAGTTTCTATGGTGCAAAGCAATATGCATCAATGATCGTGCAATTGGGTGTGTTTCTCTATCCTCAAGTTCACCTGGTGATAAATCCAGGAACAAATGTGCAGAACTTGGCTATGTTCTAGGCTCTAAATATTGGGGTAAAGGGGTTGCCACATGTGTTGTGAAACAAGTTGTTAAGGTTGCATTCTGTGAGTTGACTTACTTGGAAAGGCTTGAAGCTTTAGTTGATGTGGAAAATGCTGGGTCTCAAAGAGTGCTGGAGAAAGCTGGTTTCCAAAAGGAGGGAGTTCTAAGGAAATATTTGTTCATGAAAGGAAAAAGTAGAGATATGATCATGTCCAGTGTTCTTTCTACTGATCTCCAACTATAA

Protein sequence

>MS.gene024859.t1

MERTSISFEPRAKEERIDLTQITLRALQLSDLDDLMVWTTDEKVAKFCSWELYTSKDDGINFIENIATKFLWCKAICINDRAIGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVATCVVKQVVKVAFCELTYLERLEALVDVENAGSQRVLEKAGFQKEGVLRKYLFMKGKSRDMIMSSVLSTDLQL