AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene001033

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001033.t1 MTR_5g006460 83.041 171 24 4 1 167 1 170 1.82e-95 277
MS.gene001033.t1 MTR_5g006510 76.647 167 34 2 1 167 7 168 1.69e-88 259
MS.gene001033.t1 MTR_5g006450 72.781 169 42 3 1 167 1 167 1.74e-79 236
MS.gene001033.t1 MTR_5g006470 70.414 169 44 4 1 167 1 165 1.72e-76 229
MS.gene001033.t1 MTR_5g006520 67.262 168 53 2 1 167 1719 1885 6.86e-68 226
MS.gene001033.t1 MTR_1g103110 59.649 171 61 5 1 167 1 167 2.20e-61 191
MS.gene001033.t1 MTR_1g103150 58.480 171 63 5 1 167 1 167 2.09e-60 188
MS.gene001033.t1 MTR_7g024780 41.830 153 85 2 16 168 3 151 1.74e-31 114
MS.gene001033.t1 MTR_7g114410 40.397 151 87 1 17 167 4 151 3.70e-31 112
MS.gene001033.t1 MTR_7g114410 40.397 151 87 1 17 167 4 151 5.52e-31 112
MS.gene001033.t1 MTR_7g024760 42.282 149 79 5 20 167 7 149 3.25e-30 110
MS.gene001033.t1 MTR_7g024765 41.333 150 81 5 20 168 7 150 6.15e-30 110
MS.gene001033.t1 MTR_3g463610 38.411 151 93 0 17 167 3 153 5.47e-29 108
MS.gene001033.t1 MTR_1g103320 51.220 82 38 1 50 131 10 89 2.48e-18 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001033.t1 AT2G32020 51.316 152 63 6 20 168 18 161 4.43e-39 133
MS.gene001033.t1 AT2G32030 47.222 180 77 8 21 197 24 188 1.59e-38 132
MS.gene001033.t1 AT3G22560 35.484 155 93 3 16 167 2 152 7.16e-23 91.7

Find 52 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCAAATGTGCAGAAAT+TGG 0.173070 5.4:-2218139 MS.gene001033:CDS
ATGAAAAGGTAGCCAAGTTC+TGG 0.273400 5.4:-2218306 MS.gene001033:CDS
CTCTTCATTGGCACATGGTT+TGG 0.274557 5.4:+2218408 None:intergenic
GCTTCAAGCCTTTCCAAGTA+TGG 0.276638 5.4:+2218031 None:intergenic
ATGTTCTAGGCTCTAAATAT+TGG 0.292142 5.4:-2218114 MS.gene001033:CDS
TGTTCTAGGCTCTAAATATT+GGG 0.316974 5.4:-2218113 MS.gene001033:CDS
TTGTTGATGTGGATAATTTA+TGG 0.328127 5.4:-2218006 MS.gene001033:CDS
TGCCATAATGATCATGCTAT+TGG 0.333891 5.4:-2218202 MS.gene001033:CDS
ACCCAATAGCATGATCATTA+TGG 0.341789 5.4:+2218200 None:intergenic
GCAGAAATTGGGTATGTTCT+AGG 0.344662 5.4:-2218127 MS.gene001033:CDS
CAAATCAATGATCTCTTCAT+TGG 0.363988 5.4:+2218396 None:intergenic
CCTTCGTCTTTGCTAGTGTA+AGG 0.376096 5.4:+2218277 None:intergenic
TGTGAAGCTTGCTGCTGAAC+AGG 0.399282 5.4:+2215386 None:intergenic
ATTTGTGCGCGTAGGTGATA+TGG 0.401373 5.4:+2215296 None:intergenic
GGCTCTAAATATTGGGGTAA+AGG 0.406739 5.4:-2218106 MS.gene001033:CDS
ATTCAGTGAATTTCCATACT+TGG 0.413337 5.4:-2218044 MS.gene001033:CDS
CACGTTTGCTGTGAAACAAA+TGG 0.422199 5.4:-2218077 MS.gene001033:CDS
GCTCTAAATATTGGGGTAAA+GGG 0.428389 5.4:-2218105 MS.gene001033:CDS
TTCTGACCTTGATGATGTAA+TGG 0.440976 5.4:-2218344 MS.gene001033:CDS
CAAAGAGTAGTTGAGAAAGT+TGG 0.450786 5.4:-2217980 MS.gene001033:CDS
GAAGATGTCGGAGTGTGATT+TGG 0.453912 5.4:+2218371 None:intergenic
AAATCAAATGTGCAGAAATT+GGG 0.456981 5.4:-2218138 MS.gene001033:CDS
TGCTGTGAAACAAATGGTTA+AGG 0.473285 5.4:-2218071 MS.gene001033:CDS
GCCATAATGATCATGCTATT+GGG 0.478546 5.4:-2218201 MS.gene001033:CDS
TGAAAAGGTAGCCAAGTTCT+GGG 0.482152 5.4:-2218305 MS.gene001033:CDS
GTGAATTTCCATACTTGGAA+AGG 0.490341 5.4:-2218039 MS.gene001033:CDS
GCTTGCTGCTGAACAGGAAA+CGG 0.499122 5.4:+2215392 None:intergenic
TCCCTCCGCAGTGTATTCAC+CGG 0.533039 5.4:+2215442 None:intergenic
CCCTCCGCAGTGTATTCACC+GGG 0.566810 5.4:+2215443 None:intergenic
GCTTGAAGCTCTTGTTGATG+TGG 0.580383 5.4:-2218017 MS.gene001033:CDS
CCCGGTGAATACACTGCGGA+GGG 0.583486 5.4:-2215443 MS.gene001033:CDS
TTGTAGGCTTAAGAAACGCC+CGG 0.586951 5.4:-2215461 MS.gene001033:intron
ACGGCGTGGAATTTGGCTCT+CGG 0.591742 5.4:+2215357 None:intergenic
GTGGCAGCAGACTGATGAAA+AGG 0.594266 5.4:-2218320 MS.gene001033:CDS
GCCACACCATTACATCATCA+AGG 0.594693 5.4:+2218338 None:intergenic
ATGATCTCTTCATTGGCACA+TGG 0.598564 5.4:+2218403 None:intergenic
GATATGGTGTACTGTAGACT+TGG 0.616292 5.4:+2215312 None:intergenic
AAAAGGTAGCCAAGTTCTGG+GGG 0.620200 5.4:-2218303 MS.gene001033:CDS
ATGGTGTACTGTAGACTTGG+AGG 0.620908 5.4:+2215315 None:intergenic
GAAAAGGTAGCCAAGTTCTG+GGG 0.624494 5.4:-2218304 MS.gene001033:CDS
GATATGCCTCACAGCAAGAA+CGG 0.626057 5.4:-2215421 MS.gene001033:CDS
GTTCTAGGCTCTAAATATTG+GGG 0.628624 5.4:-2218112 MS.gene001033:CDS
GGTCAGAAAGATGAAGATGT+CGG 0.630507 5.4:+2218359 None:intergenic
GCCCGGTGAATACACTGCGG+AGG 0.637373 5.4:-2215444 MS.gene001033:CDS
TAGATCATGACGTGAACTCG+AGG 0.652639 5.4:+2218165 None:intergenic
CATTTGTTTCACAGCAAACG+TGG 0.659491 5.4:+2218078 None:intergenic
ACATCACCGTTCTTGCTGTG+AGG 0.674400 5.4:+2215415 None:intergenic
ACCTTGATGATGTAATGGTG+TGG 0.697032 5.4:-2218339 MS.gene001033:CDS
GAGCTGTCATTTGTGCGCGT+AGG 0.698927 5.4:+2215288 None:intergenic
CCTTACACTAGCAAAGACGA+AGG 0.715657 5.4:-2218277 MS.gene001033:CDS
AAAGGTAGCCAAGTTCTGGG+GGG 0.779565 5.4:-2218302 MS.gene001033:CDS
AACGCCCGGTGAATACACTG+CGG 0.820971 5.4:-2215447 MS.gene001033:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTACTTTTTTATTAATTTAC+TGG - chr5.4:2215956-2215975 MS.gene001033:intron 10.0%
!! AGGTTAATTAAATTAAAATG+TGG + chr5.4:2217658-2217677 None:intergenic 15.0%
!! TTATGGATTTCTTTATATTT+AGG + chr5.4:2216310-2216329 None:intergenic 15.0%
!!! ACTAATTTTTTGTTATCAAT+GGG + chr5.4:2217083-2217102 None:intergenic 15.0%
!!! ATTTTGTATTATTGGTATTA+TGG + chr5.4:2216422-2216441 None:intergenic 15.0%
!! AAACAAAACAAAGAATTGAT+TGG - chr5.4:2217987-2218006 MS.gene001033:CDS 20.0%
!! AAGTAATTTATGAGAATGTT+AGG - chr5.4:2217504-2217523 MS.gene001033:intron 20.0%
!! AATATGTAATCTTAAACCTT+TGG + chr5.4:2218051-2218070 None:intergenic 20.0%
!! ATATTTCATGAAATAAACCA+TGG + chr5.4:2217805-2217824 None:intergenic 20.0%
!! ATTCAGATTAAATTTCTCAT+TGG - chr5.4:2216619-2216638 MS.gene001033:intron 20.0%
!! TCATAATTGGATAAATACTT+TGG + chr5.4:2216234-2216253 None:intergenic 20.0%
!! TGCAAAAAATTTCAGTTATT+TGG + chr5.4:2216968-2216987 None:intergenic 20.0%
!!! ACATGTCTATTTTGTATTAT+TGG + chr5.4:2216430-2216449 None:intergenic 20.0%
!!! ATTTAATCTGAATTTTGTTG+TGG + chr5.4:2216612-2216631 None:intergenic 20.0%
!!! CACTAATTTTTTGTTATCAA+TGG + chr5.4:2217084-2217103 None:intergenic 20.0%
!!! CATTGATAACAAAAAATTAG+TGG - chr5.4:2217082-2217101 MS.gene001033:intron 20.0%
! AAAACAAAGAATTGATTGGA+TGG - chr5.4:2217991-2218010 MS.gene001033:CDS 25.0%
! AAAATATTATCGCATTGAGA+AGG - chr5.4:2216542-2216561 MS.gene001033:intron 25.0%
! AAAATCAAATGTGCAGAAAT+TGG - chr5.4:2215585-2215604 MS.gene001033:intron 25.0%
! AAATCAAATGTGCAGAAATT+GGG - chr5.4:2215586-2215605 MS.gene001033:intron 25.0%
! AATCTCCTATCAAGTAATTA+AGG - chr5.4:2216027-2216046 MS.gene001033:intron 25.0%
! ACACATTTCAGATAAATTCA+TGG + chr5.4:2217533-2217552 None:intergenic 25.0%
! ACATTAAAGTTATAGCTACT+TGG - chr5.4:2218171-2218190 MS.gene001033:CDS 25.0%
! AGATTGTACGATGATATATT+TGG + chr5.4:2216012-2216031 None:intergenic 25.0%
! ATGGTTATTTACACCTTATA+TGG + chr5.4:2216403-2216422 None:intergenic 25.0%
! CAAAATCATCTCTTTAACTA+TGG - chr5.4:2216656-2216675 MS.gene001033:intron 25.0%
! CAAAATTGAGAAAAGATTAG+AGG - chr5.4:2215886-2215905 MS.gene001033:intron 25.0%
! GACAAATTCAAACTTACATT+TGG - chr5.4:2217683-2217702 MS.gene001033:intron 25.0%
! GCAGAATTGAAATAAAAACA+GGG - chr5.4:2216198-2216217 MS.gene001033:intron 25.0%
! TTGTTGATGTGGATAATTTA+TGG - chr5.4:2215718-2215737 MS.gene001033:intron 25.0%
!! AAAGAGATGATTTTGCAAAA+TGG + chr5.4:2216651-2216670 None:intergenic 25.0%
!! AAGATCTTATGATAACGTAT+AGG - chr5.4:2216461-2216480 MS.gene001033:intron 25.0%
!! AAGTTTGAATTTGTCACTTA+AGG + chr5.4:2217678-2217697 None:intergenic 25.0%
!! ATAAATACTTTGGTTTAAGC+AGG + chr5.4:2216224-2216243 None:intergenic 25.0%
!! CATGACAATGTAATGTTTTA+TGG + chr5.4:2216327-2216346 None:intergenic 25.0%
!! TCTCAATGCGATAATATTTT+TGG + chr5.4:2216542-2216561 None:intergenic 25.0%
!! TTATTAATTTACTGGTTGTC+CGG - chr5.4:2215964-2215983 MS.gene001033:intron 25.0%
!! TTGGGATTTTTATATACATC+CGG - chr5.4:2217348-2217367 MS.gene001033:intron 25.0%
!!! AAAGTTGGTTTTCAAAAAGA+GGG - chr5.4:2215759-2215778 MS.gene001033:intron 25.0%
!!! CACATGAGGTTTATTTTAAA+AGG - chr5.4:2216577-2216596 MS.gene001033:intron 25.0%
!!! GCAATTTTTGAGTTATTTTC+TGG + chr5.4:2217728-2217747 None:intergenic 25.0%
AAAATTAGTGGCAGAAATGA+TGG - chr5.4:2217094-2217113 MS.gene001033:intron 30.0%
AACCATGCTTGAATGTATTT+GGG - chr5.4:2217330-2217349 MS.gene001033:intron 30.0%
AACTAAAAAGAGTTCCCAAT+AGG - chr5.4:2216154-2216173 MS.gene001033:intron 30.0%
ACGATGATATATTTGGATCA+TGG + chr5.4:2216005-2216024 None:intergenic 30.0%
ATGGTTCAATTAGATTGAAC+CGG + chr5.4:2215986-2216005 None:intergenic 30.0%
ATTCAGTGAATTTCCATACT+TGG - chr5.4:2215680-2215699 MS.gene001033:intron 30.0%
CAAATCAATGATCTCTTCAT+TGG + chr5.4:2215331-2215350 None:intergenic 30.0%
CAATGCCTTAATTACTTGAT+AGG + chr5.4:2216035-2216054 None:intergenic 30.0%
CATCTGCAGTTCAAATATAT+TGG + chr5.4:2217831-2217850 None:intergenic 30.0%
CATGCAAAATAATACTACCA+TGG - chr5.4:2218086-2218105 MS.gene001033:CDS 30.0%
GGACATCTTAAAGAAATAGT+TGG - chr5.4:2217292-2217311 MS.gene001033:intron 30.0%
GGCAGAATTGAAATAAAAAC+AGG - chr5.4:2216197-2216216 MS.gene001033:intron 30.0%
GTTGATTGTACATCAAACAA+AGG - chr5.4:2216176-2216195 MS.gene001033:intron 30.0%
TAAAATAAACCTCATGTGCA+AGG + chr5.4:2216575-2216594 None:intergenic 30.0%
TATCCAATTATGAGTGCATA+TGG - chr5.4:2216241-2216260 MS.gene001033:intron 30.0%
TATTTGAACTGCAGATGTAT+TGG - chr5.4:2217834-2217853 MS.gene001033:intron 30.0%
TCATTTCATGCACTAATTTG+AGG + chr5.4:2216772-2216791 None:intergenic 30.0%
TGATGTACAATCAACCTATT+GGG + chr5.4:2216171-2216190 None:intergenic 30.0%
TGCACTAACTACAAATTATG+TGG - chr5.4:2217158-2217177 MS.gene001033:intron 30.0%
TTGATGTACAATCAACCTAT+TGG + chr5.4:2216172-2216191 None:intergenic 30.0%
! TCATTCTCTATGACTTTTAC+AGG + chr5.4:2217756-2217775 None:intergenic 30.0%
! TGCTCTGAATTATGTGTAAA+TGG + chr5.4:2217431-2217450 None:intergenic 30.0%
! TTTGAGGTTATCAATGAGAT+TGG + chr5.4:2216756-2216775 None:intergenic 30.0%
!! ATGTTCTAGGCTCTAAATAT+TGG - chr5.4:2215610-2215629 MS.gene001033:intron 30.0%
!! GGCATTGATTTAGATTTACT+AGG - chr5.4:2216048-2216067 MS.gene001033:intron 30.0%
!! TGTTCTAGGCTCTAAATATT+GGG - chr5.4:2215611-2215630 MS.gene001033:intron 30.0%
!!! ACTATTTTGATACCGTCTAA+TGG + chr5.4:2216487-2216506 None:intergenic 30.0%
!!! ATTTTTTGCACAGTTAACCA+TGG - chr5.4:2216978-2216997 MS.gene001033:intron 30.0%
!!! GAAAGTTGGTTTTCAAAAAG+AGG - chr5.4:2215758-2215777 MS.gene001033:intron 30.0%
AAATGCTTCCACATGAATTG+AGG - chr5.4:2217038-2217057 MS.gene001033:intron 35.0%
AAGCCTACAAACAATGTGAA+TGG + chr5.4:2218253-2218272 None:intergenic 35.0%
AATGACGACTAAACATCATC+GGG - chr5.4:2217771-2217790 MS.gene001033:intron 35.0%
AATGAGCAGGTTTAATTGAG+AGG - chr5.4:2217209-2217228 MS.gene001033:intron 35.0%
ACACAGAAGCAAAGTTACAT+CGG - chr5.4:2218145-2218164 MS.gene001033:CDS 35.0%
ACCCAATAGCATGATCATTA+TGG + chr5.4:2215527-2215546 None:intergenic 35.0%
ACTTGGTTATGCTCTTTACT+GGG - chr5.4:2218188-2218207 MS.gene001033:CDS 35.0%
AGAACCTTCTTACATGTAGA+AGG - chr5.4:2216931-2216950 MS.gene001033:intron 35.0%
AGCCTACAAACAATGTGAAT+GGG + chr5.4:2218252-2218271 None:intergenic 35.0%
ATAACGTATAGGCCATTAGA+CGG - chr5.4:2216472-2216491 MS.gene001033:intron 35.0%
ATAATGAGCAGGTTTACTTG+AGG - chr5.4:2216881-2216900 MS.gene001033:intron 35.0%
ATACAAACTGCCATTCAGAA+AGG - chr5.4:2217595-2217614 MS.gene001033:intron 35.0%
ATCCCAAATACATTCAAGCA+TGG + chr5.4:2217335-2217354 None:intergenic 35.0%
ATGAGCAGGTTTAATTGAGA+GGG - chr5.4:2217210-2217229 MS.gene001033:intron 35.0%
ATTATCGCATTGAGAAGGAT+GGG - chr5.4:2216547-2216566 MS.gene001033:intron 35.0%
CAAAGAGTAGTTGAGAAAGT+TGG - chr5.4:2215744-2215763 MS.gene001033:intron 35.0%
CAACCATGCTTGAATGTATT+TGG - chr5.4:2217329-2217348 MS.gene001033:intron 35.0%
CAGAGCAGAACATTAATTAC+CGG - chr5.4:2217444-2217463 MS.gene001033:intron 35.0%
CAGTAAGGCAAATCCATATA+AGG - chr5.4:2216387-2216406 MS.gene001033:intron 35.0%
GAACCTTCTTACATGTAGAA+GGG - chr5.4:2216932-2216951 MS.gene001033:intron 35.0%
GAATGACGACTAAACATCAT+CGG - chr5.4:2217770-2217789 MS.gene001033:intron 35.0%
GCCATAATGATCATGCTATT+GGG - chr5.4:2215523-2215542 MS.gene001033:intron 35.0%
GTGAATTTCCATACTTGGAA+AGG - chr5.4:2215685-2215704 MS.gene001033:intron 35.0%
TAATGAGCAGGTTTACTTGA+GGG - chr5.4:2216882-2216901 MS.gene001033:intron 35.0%
TACTTGGTTATGCTCTTTAC+TGG - chr5.4:2218187-2218206 MS.gene001033:CDS 35.0%
TATTATCGCATTGAGAAGGA+TGG - chr5.4:2216546-2216565 MS.gene001033:intron 35.0%
TGCCATAATGATCATGCTAT+TGG - chr5.4:2215522-2215541 MS.gene001033:intron 35.0%
TGCTGTGAAACAAATGGTTA+AGG - chr5.4:2215653-2215672 MS.gene001033:intron 35.0%
TGGGAACTTTCCAATATATG+AGG - chr5.4:2217255-2217274 MS.gene001033:intron 35.0%
TTCAAAAAGAGGGAGTTCTT+AGG - chr5.4:2215769-2215788 MS.gene001033:intron 35.0%
TTCCCATTCACATTGTTTGT+AGG - chr5.4:2218247-2218266 MS.gene001033:CDS 35.0%
TTTGCCTTACTGATACTTCT+TGG + chr5.4:2216379-2216398 None:intergenic 35.0%
! ATGGTTCGTTTAAGTTTCCA+TGG + chr5.4:2218106-2218125 None:intergenic 35.0%
! CATGGTTTAATTTCGCATCA+AGG + chr5.4:2216696-2216715 None:intergenic 35.0%
! GATGAAATGAGCATTGTATG+TGG + chr5.4:2217894-2217913 None:intergenic 35.0%
! GCTCTAAATATTGGGGTAAA+GGG - chr5.4:2215619-2215638 MS.gene001033:intron 35.0%
! GTACTCTTTTATCTGTTCAG+TGG - chr5.4:2217856-2217875 MS.gene001033:intron 35.0%
! GTTTAATTTCGCATCAAGGA+TGG + chr5.4:2216692-2216711 None:intergenic 35.0%
! TAGACAGTCTAAGGTGTAAT+TGG + chr5.4:2216352-2216371 None:intergenic 35.0%
!! ATGGTTTAGTTTGTCAGCAA+GGG + chr5.4:2217016-2217035 None:intergenic 35.0%
!! GTTCTAGGCTCTAAATATTG+GGG - chr5.4:2215612-2215631 MS.gene001033:intron 35.0%
!! TTCTGACCTTGATGATGTAA+TGG - chr5.4:2215380-2215399 MS.gene001033:CDS 35.0%
!! TTGAAAGTACTTGTAGCCAA+AGG - chr5.4:2218032-2218051 MS.gene001033:CDS 35.0%
!!! CTTTGTTTTGTTTCCTCATC+TGG + chr5.4:2217980-2217999 None:intergenic 35.0%
!!! TGACTGAAGTCTTTTTTGCA+CGG + chr5.4:2218389-2218408 None:intergenic 35.0%
AACCTGCTCATTATGACAGA+TGG + chr5.4:2216875-2216894 None:intergenic 40.0%
AATGAGCAGGTTTACTTGAG+GGG - chr5.4:2216883-2216902 MS.gene001033:intron 40.0%
AGCATGCACTACATCTTATG+TGG - chr5.4:2216832-2216851 MS.gene001033:intron 40.0%
AGTCTTCTGTTACAATGAGC+AGG - chr5.4:2217196-2217215 MS.gene001033:intron 40.0%
ATACATCCGGTCTAAACCAT+GGG - chr5.4:2217361-2217380 MS.gene001033:intron 40.0%
ATATGAGGTTGCTTGCAGAA+GGG - chr5.4:2217270-2217289 MS.gene001033:intron 40.0%
ATCCATCTGTCATAATGAGC+AGG - chr5.4:2216870-2216889 MS.gene001033:intron 40.0%
ATGAAAAGGTAGCCAAGTTC+TGG - chr5.4:2215418-2215437 MS.gene001033:CDS 40.0%
ATGATCTCTTCATTGGCACA+TGG + chr5.4:2215324-2215343 None:intergenic 40.0%
CATAATCCCATGGTTTAGAC+CGG + chr5.4:2217370-2217389 None:intergenic 40.0%
CATTTGTTTCACAGCAAACG+TGG + chr5.4:2215649-2215668 None:intergenic 40.0%
CCTTACTGATACTTCTTGGA+CGG + chr5.4:2216375-2216394 None:intergenic 40.0%
CTGAATTCTTCTGCTGCTAT+TGG + chr5.4:2216719-2216738 None:intergenic 40.0%
CTGCAAGCAACCTCATATAT+TGG + chr5.4:2217268-2217287 None:intergenic 40.0%
CTGCCATATGCACTCATAAT+TGG + chr5.4:2216247-2216266 None:intergenic 40.0%
CTTGCTGACAAACTAAACCA+TGG - chr5.4:2217015-2217034 MS.gene001033:intron 40.0%
GATATGGTGTACTGTAGACT+TGG + chr5.4:2218415-2218434 None:intergenic 40.0%
GCAGAAATTGGGTATGTTCT+AGG - chr5.4:2215597-2215616 MS.gene001033:intron 40.0%
GCTCGACTCATGTATACTAT+GGG + chr5.4:2217396-2217415 None:intergenic 40.0%
GGTCAGAAAGATGAAGATGT+CGG + chr5.4:2215368-2215387 None:intergenic 40.0%
TATACATCCGGTCTAAACCA+TGG - chr5.4:2217360-2217379 MS.gene001033:intron 40.0%
TATATGAGGTTGCTTGCAGA+AGG - chr5.4:2217269-2217288 MS.gene001033:intron 40.0%
TGAAAAGGTAGCCAAGTTCT+GGG - chr5.4:2215419-2215438 MS.gene001033:CDS 40.0%
TGCTCGACTCATGTATACTA+TGG + chr5.4:2217397-2217416 None:intergenic 40.0%
TTCACTGAATCGATGAATGC+CGG + chr5.4:2217466-2217485 None:intergenic 40.0%
TTTGTTTCCTCATCTGGTGT+AGG + chr5.4:2217974-2217993 None:intergenic 40.0%
! GCACTTTTCCTCAATTCATG+TGG + chr5.4:2217049-2217068 None:intergenic 40.0%
!! ACCTTGATGATGTAATGGTG+TGG - chr5.4:2215385-2215404 MS.gene001033:CDS 40.0%
!! CACGTTTGCTGTGAAACAAA+TGG - chr5.4:2215647-2215666 MS.gene001033:intron 40.0%
!! CATGGTTTAGTTTGTCAGCA+AGG + chr5.4:2217017-2217036 None:intergenic 40.0%
!! GCATTCATCGATTCAGTGAA+TGG - chr5.4:2217466-2217485 MS.gene001033:intron 40.0%
!! GGCTCTAAATATTGGGGTAA+AGG - chr5.4:2215618-2215637 MS.gene001033:intron 40.0%
!! TTCATGTGGAAGCATTTCCA+TGG + chr5.4:2217035-2217054 None:intergenic 40.0%
ATGGTGTACTGTAGACTTGG+AGG + chr5.4:2218412-2218431 None:intergenic 45.0%
ATGTAAGAAGGTTCTCACCC+TGG + chr5.4:2216926-2216945 None:intergenic 45.0%
ATTTGTGCGCGTAGGTGATA+TGG + chr5.4:2218431-2218450 None:intergenic 45.0%
CAAATAGCTGTCAATGCCTG+TGG + chr5.4:2217634-2217653 None:intergenic 45.0%
CAACCTTACAAACACACCCA+GGG - chr5.4:2216906-2216925 MS.gene001033:intron 45.0%
CCTTACACTAGCAAAGACGA+AGG - chr5.4:2215447-2215466 MS.gene001033:CDS 45.0%
CCTTCGTCTTTGCTAGTGTA+AGG + chr5.4:2215450-2215469 None:intergenic 45.0%
CGGTCTAAACCATGGGATTA+TGG - chr5.4:2217368-2217387 MS.gene001033:intron 45.0%
CTACACAAATGAGGCACACT+TGG - chr5.4:2216270-2216289 MS.gene001033:intron 45.0%
CTCTTCATTGGCACATGGTT+TGG + chr5.4:2215319-2215338 None:intergenic 45.0%
GAAAAGGTAGCCAAGTTCTG+GGG - chr5.4:2215420-2215439 MS.gene001033:CDS 45.0%
GAACCTTAGAAACCACCTCA+GGG - chr5.4:2217232-2217251 MS.gene001033:intron 45.0%
GAAGATGTCGGAGTGTGATT+TGG + chr5.4:2215356-2215375 None:intergenic 45.0%
GATATGCCTCACAGCAAGAA+CGG - chr5.4:2218303-2218322 MS.gene001033:CDS 45.0%
GCCACACCATTACATCATCA+AGG + chr5.4:2215389-2215408 None:intergenic 45.0%
GCTTCAAGCCTTTCCAAGTA+TGG + chr5.4:2215696-2215715 None:intergenic 45.0%
GCTTGAAGCTCTTGTTGATG+TGG - chr5.4:2215707-2215726 MS.gene001033:intron 45.0%
GTGCCCTTCTACATGTAAGA+AGG + chr5.4:2216938-2216957 None:intergenic 45.0%
TAGATCATGACGTGAACTCG+AGG + chr5.4:2215562-2215581 None:intergenic 45.0%
TATGAGGTTGCTTGCAGAAG+GGG - chr5.4:2217271-2217290 MS.gene001033:intron 45.0%
TGGCAGCTTCTACACAAATG+AGG - chr5.4:2216261-2216280 MS.gene001033:intron 45.0%
TGTAAGAAGGTTCTCACCCT+GGG + chr5.4:2216925-2216944 None:intergenic 45.0%
TTCTTCTGCTGCTATTGGCA+TGG + chr5.4:2216714-2216733 None:intergenic 45.0%
TTGTAGGCTTAAGAAACGCC+CGG - chr5.4:2218263-2218282 MS.gene001033:CDS 45.0%
! AAAAGGTAGCCAAGTTCTGG+GGG - chr5.4:2215421-2215440 MS.gene001033:CDS 45.0%
! TCTTTACTGGGCATTGCATG+AGG - chr5.4:2218200-2218219 MS.gene001033:CDS 45.0%
! TTTTTGACCAGTGCGAAGCA+CGG + chr5.4:2216082-2216101 None:intergenic 45.0%
! TTTTTGCACGGCGTGGAATT+TGG + chr5.4:2218377-2218396 None:intergenic 45.0%
!! CCGTCCAAGAAGTATCAGTA+AGG - chr5.4:2216372-2216391 MS.gene001033:intron 45.0%
!! GTTTAGTTTGTCAGCAAGGG+CGG + chr5.4:2217013-2217032 None:intergenic 45.0%
AATGCCTGTGGTAAGGGATG+AGG + chr5.4:2217622-2217641 None:intergenic 50.0%
AGCTGTCAATGCCTGTGGTA+AGG + chr5.4:2217629-2217648 None:intergenic 50.0%
ATCATCGGGCATCAGATCCA+TGG - chr5.4:2217785-2217804 MS.gene001033:intron 50.0%
ATTGGAAAGTTCCCACCCTG+AGG + chr5.4:2217250-2217269 None:intergenic 50.0%
CACTTTGCCTACACCAGATG+AGG - chr5.4:2217964-2217983 MS.gene001033:CDS 50.0%
CGAACCATGAGCTAAAGCAG+CGG - chr5.4:2218118-2218137 MS.gene001033:CDS 50.0%
CTTAGAAACCACCTCAGGGT+GGG - chr5.4:2217236-2217255 MS.gene001033:intron 50.0%
CTTGGACGGTAGACAGTCTA+AGG + chr5.4:2216361-2216380 None:intergenic 50.0%
GCAACCTTACAAACACACCC+AGG - chr5.4:2216905-2216924 MS.gene001033:intron 50.0%
GCATGAGGTAATCGTCAAGC+TGG - chr5.4:2218215-2218234 MS.gene001033:CDS 50.0%
GCTGTCAATGCCTGTGGTAA+GGG + chr5.4:2217628-2217647 None:intergenic 50.0%
GGAACCTTAGAAACCACCTC+AGG - chr5.4:2217231-2217250 MS.gene001033:intron 50.0%
GGGATGAGGTCCTTTCTGAA+TGG + chr5.4:2217608-2217627 None:intergenic 50.0%
GTGGCAGCAGACTGATGAAA+AGG - chr5.4:2215404-2215423 MS.gene001033:CDS 50.0%
TCACCCTGGGTGTGTTTGTA+AGG + chr5.4:2216912-2216931 None:intergenic 50.0%
TCTGGTGTAGGCAAAGTGAC+AGG + chr5.4:2217962-2217981 None:intergenic 50.0%
! ACATCACCGTTCTTGCTGTG+AGG + chr5.4:2218312-2218331 None:intergenic 50.0%
! CTATGGGTGCCATAATCCCA+TGG + chr5.4:2217380-2217399 None:intergenic 50.0%
! GCTTGCTGCTGAACAGGAAA+CGG + chr5.4:2218335-2218354 None:intergenic 50.0%
! TGATCCGCTGCTTTAGCTCA+TGG + chr5.4:2218125-2218144 None:intergenic 50.0%
! TTTTGACCAGTGCGAAGCAC+GGG + chr5.4:2216081-2216100 None:intergenic 50.0%
!! AAAGGTAGCCAAGTTCTGGG+GGG - chr5.4:2215422-2215441 MS.gene001033:CDS 50.0%
!! TGTGAAGCTTGCTGCTGAAC+AGG + chr5.4:2218341-2218360 None:intergenic 50.0%
!!! GAAGTCTTTTTTGCACGGCG+TGG + chr5.4:2218384-2218403 None:intergenic 50.0%
AACGCCCGGTGAATACACTG+CGG - chr5.4:2218277-2218296 MS.gene001033:CDS 55.0%
AGGACCTCATCCCTTACCAC+AGG - chr5.4:2217615-2217634 MS.gene001033:intron 55.0%
CCACCCTGAGGTGGTTTCTA+AGG + chr5.4:2217238-2217257 None:intergenic 55.0%
CCTTAGAAACCACCTCAGGG+TGG - chr5.4:2217235-2217254 MS.gene001033:intron 55.0%
GTAAGGTTCCCCCCAGAACT+TGG + chr5.4:2215433-2215452 None:intergenic 55.0%
TCCCTCCGCAGTGTATTCAC+CGG + chr5.4:2218285-2218304 None:intergenic 55.0%
! GGATGGGTTCCTTGCACATG+AGG - chr5.4:2216563-2216582 MS.gene001033:intron 55.0%
!! ACGGCGTGGAATTTGGCTCT+CGG + chr5.4:2218370-2218389 None:intergenic 55.0%
CCCGGTGAATACACTGCGGA+GGG - chr5.4:2218281-2218300 MS.gene001033:CDS 60.0%
CCCTCCGCAGTGTATTCACC+GGG + chr5.4:2218284-2218303 None:intergenic 60.0%
GGAAAGTTCCCACCCTGAGG+TGG + chr5.4:2217247-2217266 None:intergenic 60.0%
AAGGGCGGTGCATGCTGCCA+TGG + chr5.4:2216998-2217017 None:intergenic 65.0%
GCCCGGTGAATACACTGCGG+AGG - chr5.4:2218280-2218299 MS.gene001033:CDS 65.0%
CGCGAACCCGTGCTTCGCAC+TGG - chr5.4:2216072-2216091 MS.gene001033:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 2215294 2218452 2215294 ID=MS.gene001033
chr5.4 mRNA 2215294 2218452 2215294 ID=MS.gene001033.t1;Parent=MS.gene001033
chr5.4 exon 2217953 2218452 2217953 ID=MS.gene001033.t1.exon1;Parent=MS.gene001033.t1
chr5.4 CDS 2217953 2218452 2217953 ID=cds.MS.gene001033.t1;Parent=MS.gene001033.t1
chr5.4 exon 2215294 2215477 2215294 ID=MS.gene001033.t1.exon2;Parent=MS.gene001033.t1
chr5.4 CDS 2215294 2215477 2215294 ID=cds.MS.gene001033.t1;Parent=MS.gene001033.t1
Gene Sequence

>MS.gene001033

ATGGAAGAGACTTCTATTTTCTCCAAACCATGTGCCAATGAAGAGATCATTGATTTGAACCAAATCACACTCCGACATCTTCATCTTTCTGACCTTGATGATGTAATGGTGTGGCAGCAGACTGATGAAAAGGTAGCCAAGTTCTGGGGGGAACCTTACACTAGCAAAGACGAAGGCATCAACTTCATTGAAAATATAGAAAGTGATTATCTACGTTGCGAAGCAATATGCCATAATGATCATGCTATTGGGTGTATTAAGTTGTCCTCGAGTTCACGTCATGATCTAAGCAAAATCAAATGTGCAGAAATTGGGTATGTTCTAGGCTCTAAATATTGGGGTAAAGGGATTACCACGTTTGCTGTGAAACAAATGGTTAAGGTTGCATTCAGTGAATTTCCATACTTGGAAAGGCTTGAAGCTCTTGTTGATGTGGATAATTTATGGTCGCAAAGAGTAGTTGAGAAAGTTGGTTTTCAAAAAGAGGGAGTTCTTAGGAAGTATGTATTTTTTAAAGAAAAAAGCAGAGATATGATCATGTTCAGTCTTCTTTCTACTGATCTTAAACTATGATTAAATTAAATATATGTATCAAAATTGAGAAAAGATTAGAGGACACTTTGCATTTATCATTTAATTTTTTACTTTTTAAACTTTTTTTTTTACTTTTTTATTAATTTACTGGTTGTCCGGTTCAATCTAATTGAACCATGATCCAAATATATCATCGTACAATCTCCTATCAAGTAATTAAGGCATTGATTTAGATTTACTAGGTCGCGAACCCGTGCTTCGCACTGGTCAAAAAAATATACATGACAAAGTCATTGTCAATTTGCATAAAAATAACAAAATAAGATAACTAAAAAGAGTTCCCAATAGGTTGATTGTACATCAAACAAAGGCAGAATTGAAATAAAAACAGGGCCTGCTTAAACCAAAGTATTTATCCAATTATGAGTGCATATGGCAGCTTCTACACAAATGAGGCACACTTGGCATACTGATAATTGCCTAAATATAAAGAAATCCATAAAACATTACATTGTCATGCACCAATTACACCTTAGACTGTCTACCGTCCAAGAAGTATCAGTAAGGCAAATCCATATAAGGTGTAAATAACCATAATACCAATAATACAAAATAGACATGTTAATTGATTTGAAGATCTTATGATAACGTATAGGCCATTAGACGGTATCAAAATAGTCATGACTTAATTTGCTATATTTTTTTTTATAACCAAAAATATTATCGCATTGAGAAGGATGGGTTCCTTGCACATGAGGTTTATTTTAAAAGGTTTACGTTACCACAACAAAATTCAGATTAAATTTCTCATTGGTTGTGACCATTTTGCAAAATCATCTCTTTAACTATGGCAGCAAATAGCCATCCTTGATGCGAAATTAAACCATGCCAATAGCAGCAGAAGAATTCAGATATGTGATGAGCACCAATCTCATTGATAACCTCAAATTAGTGCATGAAATGATGATTGCTTAAATAAAAAGAAAGCAATGACATCATAGAATAGCATGCACTACATCTTATGTGGCTAAACGTGAAAACAATCCATCTGTCATAATGAGCAGGTTTACTTGAGGGGCAACCTTACAAACACACCCAGGGTGAGAACCTTCTTACATGTAGAAGGGCACACTTTAACCAAATAACTGAAATTTTTTGCACAGTTAACCATGGCAGCATGCACCGCCCTTGCTGACAAACTAAACCATGGAAATGCTTCCACATGAATTGAGGAAAAGTGCTGAGTATGTATCCCATTGATAACAAAAAATTAGTGGCAGAAATGATGGTTGCTTAAATAAAAAGATAAACATGACAACATAAAATAGCGTGCACTAACTACAAATTATGTGGCTGAGCATGAATGCAAGTCTTCTGTTACAATGAGCAGGTTTAATTGAGAGGGAACCTTAGAAACCACCTCAGGGTGGGAACTTTCCAATATATGAGGTTGCTTGCAGAAGGGGACATCTTAAAGAAATAGTTGGAAATTTGTACAACACAACCATGCTTGAATGTATTTGGGATTTTTATATACATCCGGTCTAAACCATGGGATTATGGCACCCATAGTATACATGAGTCGAGCATCGTGATGTATCCATTTACACATAATTCAGAGCAGAACATTAATTACCGGCATTCATCGATTCAGTGAATGGTTTGATGCAACAGATAAGTAATTTATGAGAATGTTAGGTGTCCATGAATTTATCTGAAATGTGTTTGATCATGATATCTGATATACATCTGTAATTTTTTAAAGTTATACAAACTGCCATTCAGAAAGGACCTCATCCCTTACCACAGGCATTGACAGCTATTTGACCACATTTTAATTTAATTAACCTTAAGTGACAAATTCAAACTTACATTTGGTTAGTCACGAACAATGTCTCCAGAAAATAACTCAAAAATTGCATAATCCTGTAAAAGTCATAGAGAATGACGACTAAACATCATCGGGCATCAGATCCATGGTTTATTTCATGAAATATGATCCAATATATTTGAACTGCAGATGTATTGGTACTCTTTTATCTGTTCAGTGGATGCAAGCAATACCACATACAATGCTCATTTCATCAATTAGCAAGAGAGTTTCTTATTCAGAGTGTTGTATAACAACGCACCTGTCACTTTGCCTACACCAGATGAGGAAACAAAACAAAGAATTGATTGGATGGAAAGAAATCTAACATTCATTGAAAGTACTTGTAGCCAAAGGTTTAAGATTACATATTCTGACATAAAACATTCATGCAAAATAATACTACCATGGAAACTTAAACGAACCATGAGCTAAAGCAGCGGATCAACACAGAAGCAAAGTTACATCGGTAGACATTAAAGTTATAGCTACTTGGTTATGCTCTTTACTGGGCATTGCATGAGGTAATCGTCAAGCTGGTACGAATATTTCCCATTCACATTGTTTGTAGGCTTAAGAAACGCCCGGTGAATACACTGCGGAGGGATATGCCTCACAGCAAGAACGGTGATGTCCGTTTCCTGTTCAGCAGCAAGCTTCACAGCATATCCGAGAGCCAAATTCCACGCCGTGCAAAAAAGACTTCAGTCACCTCCAAGTCTACAGTACACCATATCACCTACGCGCACAAATGA
Protein sequence

>MS.gene001033.t1

MEETSIFSKPCANEEIIDLNQITLRHLHLSDLDDVMVWQQTDEKVAKFWGEPYTSKDEGINFIENIESDYLRCEAICHNDHAIGCIKLSSSSRHDLSKIKCAEIGYVLGSKYWGKGITTFAVKQMVKVAFSEFPYLERLEALVDVDNLWSQRVVEKVGFQKEGVLRKLKKRPVNTLRRDMPHSKNGDVRFLFSSKLHSISESQIPRRAKKTSVTSKSTVHHITYAHK