AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024858

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024858.t1 MTR_5g006460 84.211 171 22 4 1 167 1 170 3.21e-97 281
MS.gene024858.t1 MTR_5g006510 76.647 167 34 2 1 167 7 168 6.76e-88 258
MS.gene024858.t1 MTR_5g006450 72.781 169 42 3 1 167 1 167 4.60e-79 235
MS.gene024858.t1 MTR_5g006470 71.598 169 42 4 1 167 1 165 2.29e-78 233
MS.gene024858.t1 MTR_5g006520 67.857 168 52 2 1 167 1719 1885 5.08e-68 226
MS.gene024858.t1 MTR_1g103110 60.234 171 60 5 1 167 1 167 1.19e-61 191
MS.gene024858.t1 MTR_1g103150 59.064 171 62 5 1 167 1 167 1.90e-60 188
MS.gene024858.t1 MTR_7g024780 41.176 153 86 2 16 168 3 151 1.62e-31 114
MS.gene024858.t1 MTR_7g114410 39.735 151 88 1 17 167 4 151 1.82e-31 113
MS.gene024858.t1 MTR_7g114410 39.735 151 88 1 17 167 4 151 2.75e-31 113
MS.gene024858.t1 MTR_7g024760 40.940 149 81 4 20 167 7 149 3.55e-30 110
MS.gene024858.t1 MTR_7g024765 40.667 150 82 5 20 168 7 150 8.67e-30 109
MS.gene024858.t1 MTR_3g463610 37.748 151 94 0 17 167 3 153 8.48e-28 105
MS.gene024858.t1 MTR_1g103320 52.439 82 37 1 50 131 10 89 2.67e-19 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024858.t1 AT2G32020 51.316 152 63 6 20 168 18 161 4.78e-39 133
MS.gene024858.t1 AT2G32030 47.778 180 76 8 21 197 24 188 6.45e-38 130
MS.gene024858.t1 AT3G22560 35.484 155 93 3 16 167 2 152 8.84e-23 91.3

Find 50 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAATCAAATGTGCAGAAAT+TGG 0.218349 5.3:-1582757 MS.gene024858:CDS
ATGAAAAGGTAGCCAAGTTC+TGG 0.273400 5.3:-1582924 MS.gene024858:CDS
CTCTTCATTGGCACATGGTT+TGG 0.274557 5.3:+1583026 None:intergenic
GCTTCAAGCCTTTCCAAGTA+TGG 0.276638 5.3:+1582649 None:intergenic
ATGTTCTAGGCTCTAAATAT+TGG 0.292142 5.3:-1582732 MS.gene024858:CDS
TGTTCTAGGCTCTAAATATT+GGG 0.316974 5.3:-1582731 MS.gene024858:CDS
TTGTTGATGTGGATAATTTA+TGG 0.328127 5.3:-1582624 MS.gene024858:CDS
TGCCATAATGATCATGCTAT+TGG 0.333891 5.3:-1582820 MS.gene024858:CDS
ACCCAATAGCATGATCATTA+TGG 0.341789 5.3:+1582818 None:intergenic
GCAGAAATTGGGTATGTTCT+AGG 0.344662 5.3:-1582745 MS.gene024858:CDS
CAAATCAATGATCTCTTCAT+TGG 0.363988 5.3:+1583014 None:intergenic
CCTTCGTCTTTGCTAGTGTA+AGG 0.376096 5.3:+1582895 None:intergenic
TGTGAAGCTTGCTGCTGAAC+AGG 0.399282 5.3:+1580657 None:intergenic
ATTTGTGCGCGTAGGTGATA+TGG 0.401373 5.3:+1580567 None:intergenic
GGCTCTAAATATTGGGGTAA+AGG 0.406739 5.3:-1582724 MS.gene024858:CDS
ATTCAGTGAATTTCCATACT+TGG 0.413337 5.3:-1582662 MS.gene024858:CDS
CACGTTTGCTGTGAAACAAA+TGG 0.422199 5.3:-1582695 MS.gene024858:CDS
GCTCTAAATATTGGGGTAAA+GGG 0.428389 5.3:-1582723 MS.gene024858:CDS
TTCTGACCTTGATGATGTAA+TGG 0.440976 5.3:-1582962 MS.gene024858:CDS
CAAAGAGTAGTTGAGAAAGT+TGG 0.450786 5.3:-1582598 MS.gene024858:CDS
GAAGATGTCGGAGTGTGATT+TGG 0.453912 5.3:+1582989 None:intergenic
GAATCAAATGTGCAGAAATT+GGG 0.456981 5.3:-1582756 MS.gene024858:CDS
TGCTGTGAAACAAATGGTTA+AGG 0.473285 5.3:-1582689 MS.gene024858:CDS
GCCATAATGATCATGCTATT+GGG 0.478546 5.3:-1582819 MS.gene024858:CDS
TGAAAAGGTAGCCAAGTTCT+GGG 0.482152 5.3:-1582923 MS.gene024858:CDS
GTGAATTTCCATACTTGGAA+AGG 0.490341 5.3:-1582657 MS.gene024858:CDS
GCTTGCTGCTGAACAGGAAA+CGG 0.499122 5.3:+1580663 None:intergenic
ATGGTGTACTGTAGACTTGT+AGG 0.501883 5.3:+1580586 None:intergenic
TCCCTCCGCAGTGTATTCAC+CGG 0.533039 5.3:+1580713 None:intergenic
CCCTCCGCAGTGTATTCACC+GGG 0.566810 5.3:+1580714 None:intergenic
GCTTGAAGCTCTTGTTGATG+TGG 0.580383 5.3:-1582635 MS.gene024858:CDS
CCCGGTGAATACACTGCGGA+GGG 0.583486 5.3:-1580714 MS.gene024858:CDS
TTGTAGGCTTAAGAAACGCC+CGG 0.586951 5.3:-1580732 MS.gene024858:intron
ACGGCGTGGAATTTGGCTCT+CGG 0.591742 5.3:+1580628 None:intergenic
GTGGCAGCAGACTGATGAAA+AGG 0.594266 5.3:-1582938 MS.gene024858:CDS
GCCACACCATTACATCATCA+AGG 0.594693 5.3:+1582956 None:intergenic
ATGATCTCTTCATTGGCACA+TGG 0.598564 5.3:+1583021 None:intergenic
AAAAGGTAGCCAAGTTCTGG+GGG 0.620200 5.3:-1582921 MS.gene024858:CDS
GAAAAGGTAGCCAAGTTCTG+GGG 0.624494 5.3:-1582922 MS.gene024858:CDS
GATATGCCTCACAGCAAGAA+CGG 0.626057 5.3:-1580692 MS.gene024858:CDS
GTTCTAGGCTCTAAATATTG+GGG 0.628624 5.3:-1582730 MS.gene024858:CDS
GGTCAGAAAGATGAAGATGT+CGG 0.630507 5.3:+1582977 None:intergenic
GCCCGGTGAATACACTGCGG+AGG 0.637373 5.3:-1580715 MS.gene024858:CDS
CATTTGTTTCACAGCAAACG+TGG 0.659491 5.3:+1582696 None:intergenic
ACATCACCGTTCTTGCTGTG+AGG 0.674400 5.3:+1580686 None:intergenic
ACCTTGATGATGTAATGGTG+TGG 0.697032 5.3:-1582957 MS.gene024858:CDS
GAGCTGTCATTTGTGCGCGT+AGG 0.698927 5.3:+1580559 None:intergenic
CCTTACACTAGCAAAGACGA+AGG 0.715657 5.3:-1582895 MS.gene024858:CDS
AAAGGTAGCCAAGTTCTGGG+GGG 0.779565 5.3:-1582920 MS.gene024858:CDS
AACGCCCGGTGAATACACTG+CGG 0.820971 5.3:-1580718 MS.gene024858:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGGATTTCTTTATATTT+AGG + chr5.3:1581592-1581611 None:intergenic 15.0%
!!! ACTAATTTTTTGTTATCAAT+GGG + chr5.3:1582370-1582389 None:intergenic 15.0%
!!! ATTTTGTATTATTGGTATTA+TGG + chr5.3:1581704-1581723 None:intergenic 15.0%
!! AAGATCTTATGATAACATAT+AGG - chr5.3:1581746-1581765 MS.gene024858:intron 20.0%
!! ATTCAGATTAAATTTCTCAT+TGG - chr5.3:1581906-1581925 MS.gene024858:intron 20.0%
!! TCATAATTGGATAAATACTT+TGG + chr5.3:1581516-1581535 None:intergenic 20.0%
!! TGCAAAAAATTTCAGTTATT+TGG + chr5.3:1582255-1582274 None:intergenic 20.0%
!!! ACATGTCTATTTTGTATTAT+TGG + chr5.3:1581712-1581731 None:intergenic 20.0%
!!! ATTTAATCTGAATTTTGTTG+TGG + chr5.3:1581899-1581918 None:intergenic 20.0%
!!! CACTAATTTTTTGTTATCAA+TGG + chr5.3:1582371-1582390 None:intergenic 20.0%
!!! CATTGATAACAAAAAATTAG+TGG - chr5.3:1582369-1582388 MS.gene024858:intron 20.0%
!!! TTATTAATTTATTGGTTGTC+CGG - chr5.3:1581235-1581254 MS.gene024858:intron 20.0%
! AAAATATTATCGCATTGAGA+AGG - chr5.3:1581829-1581848 MS.gene024858:intron 25.0%
! AATCTCCTATCAAGTAATTA+AGG - chr5.3:1581298-1581317 MS.gene024858:intron 25.0%
! AGATTGTACGATGATATATT+TGG + chr5.3:1581283-1581302 None:intergenic 25.0%
! ATGGTTATTTACACCTTATA+TGG + chr5.3:1581685-1581704 None:intergenic 25.0%
! CAAAATCATCTCTTTAACTA+TGG - chr5.3:1581943-1581962 MS.gene024858:intron 25.0%
! CAAAATTGAGAAAAGATTAG+AGG - chr5.3:1581157-1581176 MS.gene024858:intron 25.0%
! GCAGAATTGAAATAAAAACA+GGG - chr5.3:1581480-1581499 MS.gene024858:intron 25.0%
! TAAGTAAAGTTATAGCTACT+TGG - chr5.3:1582789-1582808 MS.gene024858:CDS 25.0%
! TTGTTGATGTGGATAATTTA+TGG - chr5.3:1580989-1581008 MS.gene024858:intron 25.0%
!! AAAGAGATGATTTTGCAAAA+TGG + chr5.3:1581938-1581957 None:intergenic 25.0%
!! AGGAAGTATGTATTCTTTAA+AGG - chr5.3:1581060-1581079 MS.gene024858:intron 25.0%
!! ATAAATACTTTGGTTTAAGC+AGG + chr5.3:1581506-1581525 None:intergenic 25.0%
!! CATGACAATGTAATGTTTTA+TGG + chr5.3:1581609-1581628 None:intergenic 25.0%
!! TCTCAATGCGATAATATTTT+TGG + chr5.3:1581829-1581848 None:intergenic 25.0%
!!! AAAGTTGGTTTTCAAAAAGA+GGG - chr5.3:1581030-1581049 MS.gene024858:intron 25.0%
!!! CACATGAGGTTTATTTTAAA+AGG - chr5.3:1581864-1581883 MS.gene024858:intron 25.0%
AACCATGCTTGAATGTATTT+GGG - chr5.3:1582616-1582635 MS.gene024858:CDS 30.0%
ACGATGATATATTTGGATCA+TGG + chr5.3:1581276-1581295 None:intergenic 30.0%
AGAATCAAATGTGCAGAAAT+TGG - chr5.3:1580856-1580875 MS.gene024858:intron 30.0%
ATAACATATAGGCCATTAGA+CGG - chr5.3:1581757-1581776 MS.gene024858:intron 30.0%
ATGGTTCAATTAGATTGAAC+CGG + chr5.3:1581257-1581276 None:intergenic 30.0%
ATTCAGTGAATTTCCATACT+TGG - chr5.3:1580951-1580970 MS.gene024858:intron 30.0%
CAAATCAATGATCTCTTCAT+TGG + chr5.3:1580602-1580621 None:intergenic 30.0%
CAATGCCTTAATTACTTGAT+AGG + chr5.3:1581306-1581325 None:intergenic 30.0%
CAGTAAGGAAAATCCATATA+AGG - chr5.3:1581669-1581688 MS.gene024858:intron 30.0%
GAATCAAATGTGCAGAAATT+GGG - chr5.3:1580857-1580876 MS.gene024858:intron 30.0%
GATAACAAAAAATTAGTGGC+AGG - chr5.3:1582373-1582392 MS.gene024858:intron 30.0%
GGACATCTTAAAGAAATAGT+TGG - chr5.3:1582579-1582598 MS.gene024858:CDS 30.0%
GGCAGAATTGAAATAAAAAC+AGG - chr5.3:1581479-1581498 MS.gene024858:intron 30.0%
GTTGATTGTACATCAAACAA+AGG - chr5.3:1581458-1581477 MS.gene024858:intron 30.0%
TAAAATAAACCTCATGTGCA+AGG + chr5.3:1581862-1581881 None:intergenic 30.0%
TATCCAATTATGAGTGCATA+TGG - chr5.3:1581523-1581542 MS.gene024858:intron 30.0%
TCATTTCATGCACTAATTTG+AGG + chr5.3:1582059-1582078 None:intergenic 30.0%
TGATGTACAATCAACCTATT+GGG + chr5.3:1581453-1581472 None:intergenic 30.0%
TGCACTAACTACAAATTATG+TGG - chr5.3:1582445-1582464 MS.gene024858:intron 30.0%
TTGATGTACAATCAACCTAT+TGG + chr5.3:1581454-1581473 None:intergenic 30.0%
! TGCTCTGAATTATGTGTAAA+TGG + chr5.3:1582717-1582736 None:intergenic 30.0%
! TTTGAGGTTATCAATGAGAT+TGG + chr5.3:1582043-1582062 None:intergenic 30.0%
! TTTTCCTTACTGATACTTCT+TGG + chr5.3:1581661-1581680 None:intergenic 30.0%
!! ATGTTCTAGGCTCTAAATAT+TGG - chr5.3:1580881-1580900 MS.gene024858:intron 30.0%
!! GGCATTGATTTAGATTTACT+AGG - chr5.3:1581319-1581338 MS.gene024858:intron 30.0%
!! TGTTCTAGGCTCTAAATATT+GGG - chr5.3:1580882-1580901 MS.gene024858:intron 30.0%
!! TTGGGAGTTTTATATACATC+CGG - chr5.3:1582634-1582653 MS.gene024858:CDS 30.0%
!!! ACTATTTTGATACCGTCTAA+TGG + chr5.3:1581772-1581791 None:intergenic 30.0%
!!! ATTTTTTGCACAGTTAACCA+TGG - chr5.3:1582265-1582284 MS.gene024858:intron 30.0%
!!! GAAAGTTGGTTTTCAAAAAG+AGG - chr5.3:1581029-1581048 MS.gene024858:intron 30.0%
AAAATTAGTGGCAGGAATGA+TGG - chr5.3:1582381-1582400 MS.gene024858:intron 35.0%
AAATGCTTCCACATGAATTG+AGG - chr5.3:1582325-1582344 MS.gene024858:intron 35.0%
AACTAAGAAGAGTTCCCAAT+AGG - chr5.3:1581436-1581455 MS.gene024858:intron 35.0%
AAGCCTACAAACAATGTGAA+TGG + chr5.3:1582871-1582890 None:intergenic 35.0%
AATGAGCAGGTTTAATTGAG+AGG - chr5.3:1582496-1582515 MS.gene024858:intron 35.0%
ACCCAATAGCATGATCATTA+TGG + chr5.3:1580798-1580817 None:intergenic 35.0%
AGAACCTTCTTACATGTAGA+AGG - chr5.3:1582218-1582237 MS.gene024858:intron 35.0%
AGCCTACAAACAATGTGAAT+GGG + chr5.3:1582870-1582889 None:intergenic 35.0%
ATAATGAGCAGGTTTACTTG+AGG - chr5.3:1582168-1582187 MS.gene024858:intron 35.0%
ATGAGCAGGTTTAATTGAGA+GGG - chr5.3:1582497-1582516 MS.gene024858:intron 35.0%
ATTATCGCATTGAGAAGGAT+GGG - chr5.3:1581834-1581853 MS.gene024858:intron 35.0%
CAAAGAGTAGTTGAGAAAGT+TGG - chr5.3:1581015-1581034 MS.gene024858:intron 35.0%
CAACCATGCTTGAATGTATT+TGG - chr5.3:1582615-1582634 MS.gene024858:CDS 35.0%
CAGAGCAGAACATTAATTAC+CGG - chr5.3:1582730-1582749 MS.gene024858:CDS 35.0%
GAACCTTCTTACATGTAGAA+GGG - chr5.3:1582219-1582238 MS.gene024858:intron 35.0%
GCCATAATGATCATGCTATT+GGG - chr5.3:1580794-1580813 MS.gene024858:intron 35.0%
GTGAATTTCCATACTTGGAA+AGG - chr5.3:1580956-1580975 MS.gene024858:intron 35.0%
TAATGAGCAGGTTTACTTGA+GGG - chr5.3:1582169-1582188 MS.gene024858:intron 35.0%
TATTATCGCATTGAGAAGGA+TGG - chr5.3:1581833-1581852 MS.gene024858:intron 35.0%
TGCCATAATGATCATGCTAT+TGG - chr5.3:1580793-1580812 MS.gene024858:intron 35.0%
TGCTGTGAAACAAATGGTTA+AGG - chr5.3:1580924-1580943 MS.gene024858:intron 35.0%
TGGGAACTTTCCAATATATG+AGG - chr5.3:1582542-1582561 MS.gene024858:intron 35.0%
TTCAAAAAGAGGGAGTTCTT+AGG - chr5.3:1581040-1581059 MS.gene024858:intron 35.0%
TTCCCATTCACATTGTTTGT+AGG - chr5.3:1582865-1582884 MS.gene024858:CDS 35.0%
! CATGGTTTAATTTCGCATCA+AGG + chr5.3:1581983-1582002 None:intergenic 35.0%
! GCTCTAAATATTGGGGTAAA+GGG - chr5.3:1580890-1580909 MS.gene024858:intron 35.0%
! GTTTAATTTCGCATCAAGGA+TGG + chr5.3:1581979-1581998 None:intergenic 35.0%
! TAGACAGTCTAAGGTGTAAT+TGG + chr5.3:1581634-1581653 None:intergenic 35.0%
!! ATGGTTTAGTTTGTCAGCAA+GGG + chr5.3:1582303-1582322 None:intergenic 35.0%
!! GTTCTAGGCTCTAAATATTG+GGG - chr5.3:1580883-1580902 MS.gene024858:intron 35.0%
!! TTCTGACCTTGATGATGTAA+TGG - chr5.3:1580651-1580670 MS.gene024858:CDS 35.0%
!!! TGACTGAAGTCTTTTTTGCA+CGG + chr5.3:1583007-1583026 None:intergenic 35.0%
AACCTGCTCATTATGACAGA+TGG + chr5.3:1582162-1582181 None:intergenic 40.0%
AATGAGCAGGTTTACTTGAG+GGG - chr5.3:1582170-1582189 MS.gene024858:intron 40.0%
ACTTGGTTGTGCTCTTTACT+GGG - chr5.3:1582806-1582825 MS.gene024858:CDS 40.0%
AGCATGCACTACATCTTATG+TGG - chr5.3:1582119-1582138 MS.gene024858:intron 40.0%
AGTCTTCTGTTACAATGAGC+AGG - chr5.3:1582483-1582502 MS.gene024858:intron 40.0%
ATACATCCGGTCTAAACCAT+GGG - chr5.3:1582647-1582666 MS.gene024858:CDS 40.0%
ATATGAGGTTGCTTGCAGAA+GGG - chr5.3:1582557-1582576 MS.gene024858:intron 40.0%
ATCCATCTGTCATAATGAGC+AGG - chr5.3:1582157-1582176 MS.gene024858:intron 40.0%
ATGAAAAGGTAGCCAAGTTC+TGG - chr5.3:1580689-1580708 MS.gene024858:CDS 40.0%
ATGATCTCTTCATTGGCACA+TGG + chr5.3:1580595-1580614 None:intergenic 40.0%
ATGGTGTACTGTAGACTTGT+AGG + chr5.3:1583030-1583049 None:intergenic 40.0%
CATAATCCCATGGTTTAGAC+CGG + chr5.3:1582656-1582675 None:intergenic 40.0%
CATTTGTTTCACAGCAAACG+TGG + chr5.3:1580920-1580939 None:intergenic 40.0%
CCTTACTGATACTTCTTGGA+CGG + chr5.3:1581657-1581676 None:intergenic 40.0%
CTCCCAAATACATTCAAGCA+TGG + chr5.3:1582621-1582640 None:intergenic 40.0%
CTGAATTCTTCTGCTGCTAT+TGG + chr5.3:1582006-1582025 None:intergenic 40.0%
CTGCAAGCAACCTCATATAT+TGG + chr5.3:1582555-1582574 None:intergenic 40.0%
CTGCCATATGCACTCATAAT+TGG + chr5.3:1581529-1581548 None:intergenic 40.0%
CTTGCTGACAAACTAAACCA+TGG - chr5.3:1582302-1582321 MS.gene024858:intron 40.0%
GCAGAAATTGGGTATGTTCT+AGG - chr5.3:1580868-1580887 MS.gene024858:intron 40.0%
GCTCGACTCATGTATACTAT+GGG + chr5.3:1582682-1582701 None:intergenic 40.0%
GGTCAGAAAGATGAAGATGT+CGG + chr5.3:1580639-1580658 None:intergenic 40.0%
TACTTGGTTGTGCTCTTTAC+TGG - chr5.3:1582805-1582824 MS.gene024858:CDS 40.0%
TATACATCCGGTCTAAACCA+TGG - chr5.3:1582646-1582665 MS.gene024858:CDS 40.0%
TATATGAGGTTGCTTGCAGA+AGG - chr5.3:1582556-1582575 MS.gene024858:intron 40.0%
TGAAAAGGTAGCCAAGTTCT+GGG - chr5.3:1580690-1580709 MS.gene024858:CDS 40.0%
TGCTCGACTCATGTATACTA+TGG + chr5.3:1582683-1582702 None:intergenic 40.0%
TTCACTGAATCGATGAATGC+CGG + chr5.3:1582752-1582771 None:intergenic 40.0%
! GCACTTTTCCTCAATTCATG+TGG + chr5.3:1582336-1582355 None:intergenic 40.0%
!! ACCTTGATGATGTAATGGTG+TGG - chr5.3:1580656-1580675 MS.gene024858:CDS 40.0%
!! CACGTTTGCTGTGAAACAAA+TGG - chr5.3:1580918-1580937 MS.gene024858:intron 40.0%
!! CATGGTTTAGTTTGTCAGCA+AGG + chr5.3:1582304-1582323 None:intergenic 40.0%
!! GCATTCATCGATTCAGTGAA+TGG - chr5.3:1582752-1582771 MS.gene024858:CDS 40.0%
!! GGCTCTAAATATTGGGGTAA+AGG - chr5.3:1580889-1580908 MS.gene024858:intron 40.0%
!! TTCATGTGGAAGCATTTCCA+TGG + chr5.3:1582322-1582341 None:intergenic 40.0%
ATGTAAGAAGGTTCTCACCC+TGG + chr5.3:1582213-1582232 None:intergenic 45.0%
ATTTGTGCGCGTAGGTGATA+TGG + chr5.3:1583049-1583068 None:intergenic 45.0%
CAACCTTACAAACACACCCA+GGG - chr5.3:1582193-1582212 MS.gene024858:intron 45.0%
CCTTACACTAGCAAAGACGA+AGG - chr5.3:1580718-1580737 MS.gene024858:CDS 45.0%
CCTTCGTCTTTGCTAGTGTA+AGG + chr5.3:1580721-1580740 None:intergenic 45.0%
CGGTCTAAACCATGGGATTA+TGG - chr5.3:1582654-1582673 MS.gene024858:CDS 45.0%
CTACACAAATGAGGCACACT+TGG - chr5.3:1581552-1581571 MS.gene024858:intron 45.0%
CTCTTCATTGGCACATGGTT+TGG + chr5.3:1580590-1580609 None:intergenic 45.0%
GAAAAGGTAGCCAAGTTCTG+GGG - chr5.3:1580691-1580710 MS.gene024858:CDS 45.0%
GAACCTTAGAAACCACCTCA+GGG - chr5.3:1582519-1582538 MS.gene024858:intron 45.0%
GAAGATGTCGGAGTGTGATT+TGG + chr5.3:1580627-1580646 None:intergenic 45.0%
GATATGCCTCACAGCAAGAA+CGG - chr5.3:1582921-1582940 MS.gene024858:CDS 45.0%
GCCACACCATTACATCATCA+AGG + chr5.3:1580660-1580679 None:intergenic 45.0%
GCTTCAAGCCTTTCCAAGTA+TGG + chr5.3:1580967-1580986 None:intergenic 45.0%
GCTTGAAGCTCTTGTTGATG+TGG - chr5.3:1580978-1580997 MS.gene024858:intron 45.0%
GTGCCCTTCTACATGTAAGA+AGG + chr5.3:1582225-1582244 None:intergenic 45.0%
TATGAGGTTGCTTGCAGAAG+GGG - chr5.3:1582558-1582577 MS.gene024858:intron 45.0%
TGGCAGCTTCTACACAAATG+AGG - chr5.3:1581543-1581562 MS.gene024858:intron 45.0%
TGTAAGAAGGTTCTCACCCT+GGG + chr5.3:1582212-1582231 None:intergenic 45.0%
TTCTTCTGCTGCTATTGGCA+TGG + chr5.3:1582001-1582020 None:intergenic 45.0%
TTGTAGGCTTAAGAAACGCC+CGG - chr5.3:1582881-1582900 MS.gene024858:CDS 45.0%
! AAAAGGTAGCCAAGTTCTGG+GGG - chr5.3:1580692-1580711 MS.gene024858:CDS 45.0%
! TCTTTACTGGGCATTGCATG+AGG - chr5.3:1582818-1582837 MS.gene024858:CDS 45.0%
! TTTTTGCACGGCGTGGAATT+TGG + chr5.3:1582995-1583014 None:intergenic 45.0%
!! CCGTCCAAGAAGTATCAGTA+AGG - chr5.3:1581654-1581673 MS.gene024858:intron 45.0%
!! GTTTAGTTTGTCAGCAAGGG+CGG + chr5.3:1582300-1582319 None:intergenic 45.0%
!!! TTACTTTTTTATTAATTTAT+TGG - chr5.3:1581227-1581246 MS.gene024858:intron 5.0%
ATTGGAAAGTTCCCACCCTG+AGG + chr5.3:1582537-1582556 None:intergenic 50.0%
CTTAGAAACCACCTCAGGGT+GGG - chr5.3:1582523-1582542 MS.gene024858:intron 50.0%
CTTGGACGGTAGACAGTCTA+AGG + chr5.3:1581643-1581662 None:intergenic 50.0%
GCAACCTTACAAACACACCC+AGG - chr5.3:1582192-1582211 MS.gene024858:intron 50.0%
GCATGAGGTAATCGTCAAGC+TGG - chr5.3:1582833-1582852 MS.gene024858:CDS 50.0%
GGAACCTTAGAAACCACCTC+AGG - chr5.3:1582518-1582537 MS.gene024858:intron 50.0%
GTGGCAGCAGACTGATGAAA+AGG - chr5.3:1580675-1580694 MS.gene024858:CDS 50.0%
TCACCCTGGGTGTGTTTGTA+AGG + chr5.3:1582199-1582218 None:intergenic 50.0%
! ACATCACCGTTCTTGCTGTG+AGG + chr5.3:1582930-1582949 None:intergenic 50.0%
! CTATGGGTGCCATAATCCCA+TGG + chr5.3:1582666-1582685 None:intergenic 50.0%
! GCTTGCTGCTGAACAGGAAA+CGG + chr5.3:1582953-1582972 None:intergenic 50.0%
!! AAAGGTAGCCAAGTTCTGGG+GGG - chr5.3:1580693-1580712 MS.gene024858:CDS 50.0%
!! TGTGAAGCTTGCTGCTGAAC+AGG + chr5.3:1582959-1582978 None:intergenic 50.0%
!!! GAAGTCTTTTTTGCACGGCG+TGG + chr5.3:1583002-1583021 None:intergenic 50.0%
AACGCCCGGTGAATACACTG+CGG - chr5.3:1582895-1582914 MS.gene024858:CDS 55.0%
ACGTTGACCAGTGCGAAGCA+CGG + chr5.3:1581353-1581372 None:intergenic 55.0%
CCACCCTGAGGTGGTTTCTA+AGG + chr5.3:1582525-1582544 None:intergenic 55.0%
CCTTAGAAACCACCTCAGGG+TGG - chr5.3:1582522-1582541 MS.gene024858:intron 55.0%
GTAAGGTTCCCCCCAGAACT+TGG + chr5.3:1580704-1580723 None:intergenic 55.0%
TCCCTCCGCAGTGTATTCAC+CGG + chr5.3:1582903-1582922 None:intergenic 55.0%
! GGATGGGTTCCTTGCACATG+AGG - chr5.3:1581850-1581869 MS.gene024858:intron 55.0%
!! ACGGCGTGGAATTTGGCTCT+CGG + chr5.3:1582988-1583007 None:intergenic 55.0%
CCCGGTGAATACACTGCGGA+GGG - chr5.3:1582899-1582918 MS.gene024858:CDS 60.0%
CCCTCCGCAGTGTATTCACC+GGG + chr5.3:1582902-1582921 None:intergenic 60.0%
CGTTGACCAGTGCGAAGCAC+GGG + chr5.3:1581352-1581371 None:intergenic 60.0%
GGAAAGTTCCCACCCTGAGG+TGG + chr5.3:1582534-1582553 None:intergenic 60.0%
AAGGGCGGTGCATGCTGCCA+TGG + chr5.3:1582285-1582304 None:intergenic 65.0%
GCCCGGTGAATACACTGCGG+AGG - chr5.3:1582898-1582917 MS.gene024858:CDS 65.0%
CGCGAACCCGTGCTTCGCAC+TGG - chr5.3:1581343-1581362 MS.gene024858:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 1580565 1583070 1580565 ID=MS.gene024858
chr5.3 mRNA 1580565 1583070 1580565 ID=MS.gene024858.t1;Parent=MS.gene024858
chr5.3 exon 1582571 1583070 1582571 ID=MS.gene024858.t1.exon1;Parent=MS.gene024858.t1
chr5.3 CDS 1582571 1583070 1582571 ID=cds.MS.gene024858.t1;Parent=MS.gene024858.t1
chr5.3 exon 1580565 1580748 1580565 ID=MS.gene024858.t1.exon2;Parent=MS.gene024858.t1
chr5.3 CDS 1580565 1580748 1580565 ID=cds.MS.gene024858.t1;Parent=MS.gene024858.t1
Gene Sequence

>MS.gene024858

ATGGAAGAGACTTCTATTTTCTCCAAACCATGTGCCAATGAAGAGATCATTGATTTGAACCAAATCACACTCCGACATCTTCATCTTTCTGACCTTGATGATGTAATGGTGTGGCAGCAGACTGATGAAAAGGTAGCCAAGTTCTGGGGGGAACCTTACACTAGCAAAGACGAAGGCATCAACTTCATTGAAAATATAGAAAGTGATTATCTACGTTGCGAAGCAATATGCCATAATGATCATGCTATTGGGTGTATTAAGTTGTTCTCGAGTTCACGTCATGATCTAAGCAGAATCAAATGTGCAGAAATTGGGTATGTTCTAGGCTCTAAATATTGGGGTAAAGGGATTACCACGTTTGCTGTGAAACAAATGGTTAAGGTTGCATTCAGTGAATTTCCATACTTGGAAAGGCTTGAAGCTCTTGTTGATGTGGATAATTTATGGTCGCAAAGAGTAGTTGAGAAAGTTGGTTTTCAAAAAGAGGGAGTTCTTAGGAAGTATGTATTCTTTAAAGGAAAAAGCAGAGATATGATCATGTTCAGTCTTCTTTCTACTGATCTTAAACTATGATTAAATTAAATATATGTATCAAAATTGAGAAAAGATTAGAGGACACTTTGCATTTATCATTTAATTTTTTACTTTTTAAACTTTTTTTTTTACTTTTTTATTAATTTATTGGTTGTCCGGTTCAATCTAATTGAACCATGATCCAAATATATCATCGTACAATCTCCTATCAAGTAATTAAGGCATTGATTTAGATTTACTAGGTCGCGAACCCGTGCTTCGCACTGGTCAACGTTGTCTGCAAAAAATATACATGACAAAGTCATTGTCAATTTGCATAAAAATAACAAAATAAGATAACTAAGAAGAGTTCCCAATAGGTTGATTGTACATCAAACAAAGGCAGAATTGAAATAAAAACAGGGCCTGCTTAAACCAAAGTATTTATCCAATTATGAGTGCATATGGCAGCTTCTACACAAATGAGGCACACTTGGCATACTGATAATTGCCTAAATATAAAGAAATCCATAAAACATTACATTGTCATGCACCAATTACACCTTAGACTGTCTACCGTCCAAGAAGTATCAGTAAGGAAAATCCATATAAGGTGTAAATAACCATAATACCAATAATACAAAATAGACATGTTAATTGATTTGAAGAAGATCTTATGATAACATATAGGCCATTAGACGGTATCAAAATAGTCATGACTTAATTTACTATATTTTTTTTTTTATAACCAAAAATATTATCGCATTGAGAAGGATGGGTTCCTTGCACATGAGGTTTATTTTAAAAGGTTTACGTTACCACAACAAAATTCAGATTAAATTTCTCATTGGTTGTGACCATTTTGCAAAATCATCTCTTTAACTATGGCAGCAAATAGCCATCCTTGATGCGAAATTAAACCATGCCAATAGCAGCAGAAGAATTCAGATATGTGATGAGCACCAATCTCATTGATAACCTCAAATTAGTGCATGAAATGATGATTGCTTAAATAAAAAGAAAGCAATGACATCATAGAATAGCATGCACTACATCTTATGTGGCTAAACGTGAAAACAATCCATCTGTCATAATGAGCAGGTTTACTTGAGGGGCAACCTTACAAACACACCCAGGGTGAGAACCTTCTTACATGTAGAAGGGCACACTTTAACCAAATAACTGAAATTTTTTGCACAGTTAACCATGGCAGCATGCACCGCCCTTGCTGACAAACTAAACCATGGAAATGCTTCCACATGAATTGAGGAAAAGTGCTGAGTATGTATCCCATTGATAACAAAAAATTAGTGGCAGGAATGATGGTTGCTTAAATAAAAAGATAAACATGACAACATAAAATAGCGTGCACTAACTACAAATTATGTGGCTGAGCATGAATGCAAGTCTTCTGTTACAATGAGCAGGTTTAATTGAGAGGGAACCTTAGAAACCACCTCAGGGTGGGAACTTTCCAATATATGAGGTTGCTTGCAGAAGGGGACATCTTAAAGAAATAGTTGGAAATTGTACAACACAACCATGCTTGAATGTATTTGGGAGTTTTATATACATCCGGTCTAAACCATGGGATTATGGCACCCATAGTATACATGAGTCGAGCATCGTGATGTATCCATTTACACATAATTCAGAGCAGAACATTAATTACCGGCATTCATCGATTCAGTGAATGGTTTGATGCAACAGATAAGTAAAGTTATAGCTACTTGGTTGTGCTCTTTACTGGGCATTGCATGAGGTAATCGTCAAGCTGGTACGAATATTTCCCATTCACATTGTTTGTAGGCTTAAGAAACGCCCGGTGAATACACTGCGGAGGGATATGCCTCACAGCAAGAACGGTGATGTCCGTTTCCTGTTCAGCAGCAAGCTTCACAGCATATCCGAGAGCCAAATTCCACGCCGTGCAAAAAAGACTTCAGTCACCTACAAGTCTACAGTACACCATATCACCTACGCGCACAAATGA
Protein sequence

>MS.gene024858.t1

MEETSIFSKPCANEEIIDLNQITLRHLHLSDLDDVMVWQQTDEKVAKFWGEPYTSKDEGINFIENIESDYLRCEAICHNDHAIGCIKLFSSSRHDLSRIKCAEIGYVLGSKYWGKGITTFAVKQMVKVAFSEFPYLERLEALVDVDNLWSQRVVEKVGFQKEGVLRKLKKRPVNTLRRDMPHSKNGDVRFLFSSKLHSISESQIPRRAKKTSVTYKSTVHHITYAHK