AlfalfaGEDB | MS.gene024854

M. sativa cultivar XinJiangDaYe / MS.gene024854

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024854.t1	XP_024639963.1	92.6	190	14	0	1	190	1	190	2.30E-95	358.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024854.t1	P05332	31.8	173	107	3	21	187	8	175	4.1e-16	86.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024854.t1	G7K3P8	92.6	190	14	0	1	190	1719	1908	1.7e-95	358.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene024854.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene001038	MS.gene024854	PPI
MS.gene21830	MS.gene024854	PPI
MS.gene024854	MS.gene74082	PPI
MS.gene21833	MS.gene024854	PPI
MS.gene043815	MS.gene024854	PPI
MS.gene024854	MS.gene024857	PPI
MS.gene024854	MS.gene95801	PPI
MS.gene29937	MS.gene024854	PPI
MS.gene024858	MS.gene024854	PPI
MS.gene024854	MS.gene001037	PPI
MS.gene024854	MS.gene21833	PPI
MS.gene006418	MS.gene024854	PPI
MS.gene024854	MS.gene071357	PPI
MS.gene024854	MS.gene21828	PPI
MS.gene024854	MS.gene001033	PPI
MS.gene024854	MS.gene024859	PPI
MS.gene024854	MS.gene95802	PPI
MS.gene024854	MS.gene001032	PPI
MS.gene024854	MS.gene49471	PPI
MS.gene024854	MS.gene006418	PPI
MS.gene024854	MS.gene024614	PPI
MS.gene024854	MS.gene001038	PPI
MS.gene001033	MS.gene024854	PPI
MS.gene024854	MS.gene21830	PPI
MS.gene024854	MS.gene050493	PPI
MS.gene49468	MS.gene024854	PPI
MS.gene024854	MS.gene001034	PPI
MS.gene024854	MS.gene070945	PPI
MS.gene024854	MS.gene22888	PPI
MS.gene024854	MS.gene04530	PPI
MS.gene024854	MS.gene071361	PPI
MS.gene024854	MS.gene043813	PPI
MS.gene024854	MS.gene21831	PPI
MS.gene024854	MS.gene024612	PPI
MS.gene024854	MS.gene34371	PPI
MS.gene024854	MS.gene006417	PPI
MS.gene024854	MS.gene21827	PPI
MS.gene95802	MS.gene024854	PPI
MS.gene024854	MS.gene070944	PPI
MS.gene34370	MS.gene024854	PPI
MS.gene49472	MS.gene024854	PPI
MS.gene024855	MS.gene024854	PPI
MS.gene77154	MS.gene024854	PPI
MS.gene024854	MS.gene49470	PPI
MS.gene024854	MS.gene77154	PPI
MS.gene024854	MS.gene49468	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024854.t1	MTR_5g006520	92.632	190	14	0	1	190	1719	1908	1.55e-121	378
MS.gene024854.t1	MTR_5g006450	78.421	190	41	0	1	190	1	190	1.49e-107	306
MS.gene024854.t1	MTR_5g006470	75.789	190	44	1	1	190	1	188	3.98e-103	295
MS.gene024854.t1	MTR_5g006460	70.256	195	51	5	1	190	1	193	7.91e-91	264
MS.gene024854.t1	MTR_5g006510	67.539	191	55	3	1	190	7	191	4.71e-87	254
MS.gene024854.t1	MTR_1g103110	61.458	192	70	2	1	190	1	190	5.91e-83	244
MS.gene024854.t1	MTR_1g103150	59.375	192	74	2	1	190	1	190	6.23e-80	236
MS.gene024854.t1	MTR_7g114410	42.105	171	96	1	17	187	4	171	2.76e-47	153
MS.gene024854.t1	MTR_3g463610	38.953	172	105	0	16	187	2	173	9.44e-45	147
MS.gene024854.t1	MTR_7g114410	41.667	156	88	1	17	172	4	156	3.30e-42	139
MS.gene024854.t1	MTR_7g024760	41.071	168	94	3	20	187	7	169	2.35e-38	130
MS.gene024854.t1	MTR_7g024780	39.080	174	96	3	17	187	4	170	3.37e-37	127
MS.gene024854.t1	MTR_7g024765	39.881	168	96	3	20	187	7	169	1.82e-35	122
MS.gene024854.t1	MTR_1g103320	55.556	81	34	1	51	131	11	89	6.23e-22	86.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024854.t1	AT2G32030	51.190	168	75	3	21	187	24	185	2.45e-58	181
MS.gene024854.t1	AT2G32020	46.561	189	84	4	2	187	6	180	3.15e-54	171
MS.gene024854.t1	AT3G22560	40.237	169	96	2	21	187	7	172	3.01e-37	127

Find 38 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCTAAGAATTCCTTCCTTC+TGG	0.242611	5.3:+1598399	None:intergenic
ACAGAACTTGCCTATGTTCT+AGG	0.250597	5.3:-1598572	MS.gene024854:CDS
GTTGATGCCTTGTTCTTTGC+TGG	0.260002	5.3:+1598715	None:intergenic
ATGTTCTAGGATCTAAATAT+TGG	0.302360	5.3:-1598559	MS.gene024854:CDS
CCTTGTTCTTTGCTGGTGTA+TGG	0.318147	5.3:+1598722	None:intergenic
TGTTCTAGGATCTAAATATT+GGG	0.318462	5.3:-1598558	MS.gene024854:CDS
TGCCTTAATGATCGTGCTAT+TGG	0.357294	5.3:-1598647	MS.gene024854:CDS
ATATACCAAACAAATTTCTA+TGG	0.388477	5.3:-1598682	MS.gene024854:CDS
TCCAGAAGGAAGGAATTCTT+AGG	0.397920	5.3:-1598400	MS.gene024854:CDS
GGCCAATAGCACGATCATTA+AGG	0.403869	5.3:+1598645	None:intergenic
TTGCACCATAGAAATTTGTT+TGG	0.404833	5.3:+1598677	None:intergenic
TTGTTGATGTTGAAAATGTT+GGG	0.408191	5.3:-1598451	MS.gene024854:CDS
GAAAAGGTCGGAGTGCGATT+TGG	0.410859	5.3:+1598816	None:intergenic
CCAAACAAATAAATCATAGT+TGG	0.419913	5.3:+1598312	None:intergenic
TTCTGACCTTGATGAGCTCC+TGG	0.433077	5.3:-1598789	MS.gene024854:CDS
TTTGTTGATGTTGAAAATGT+TGG	0.442167	5.3:-1598452	MS.gene024854:CDS
AGTGGCCAAGTTCTGCTCTT+GGG	0.462852	5.3:-1598747	MS.gene024854:CDS
AAGTGGCCAAGTTCTGCTCT+TGG	0.474319	5.3:-1598748	MS.gene024854:CDS
GGATCTAAATATTGGGGTAA+AGG	0.475846	5.3:-1598551	MS.gene024854:CDS
AGGAAGTATTTGTTCATGAA+AGG	0.475944	5.3:-1598380	MS.gene024854:CDS
TTTCATCAGCTCTCCATACC+AGG	0.488476	5.3:+1598771	None:intergenic
CAAAGAGTGTTGGAGAAAGC+TGG	0.497277	5.3:-1598425	MS.gene024854:CDS
TCAAGGTCAGAAAGATGAAA+AGG	0.513299	5.3:+1598800	None:intergenic
ATATTTAGATCCTAGAACAT+AGG	0.519203	5.3:+1598562	None:intergenic
ACCTTGATGAGCTCCTGGTA+TGG	0.539184	5.3:-1598784	MS.gene024854:CDS
GATCACCTCATGATAAAAGC+AGG	0.555612	5.3:-1598604	MS.gene024854:CDS
TCCATACCAGGAGCTCATCA+AGG	0.558283	5.3:+1598783	None:intergenic
TGTTGGGTCTCAAAGAGTGT+TGG	0.566749	5.3:-1598435	MS.gene024854:CDS
TGGTTCCCAAGAGCAGAACT+TGG	0.574822	5.3:+1598742	None:intergenic
GTTCTAGGATCTAAATATTG+GGG	0.601221	5.3:-1598557	MS.gene024854:CDS
CCATACACCAGCAAAGAACA+AGG	0.601334	5.3:-1598722	MS.gene024854:CDS
GGAGAAAGCTGGTTTCCAGA+AGG	0.617484	5.3:-1598414	MS.gene024854:CDS
AACTTGTTTCACAACATACG+TGG	0.623050	5.3:+1598523	None:intergenic
AAAGCTGGTTTCCAGAAGGA+AGG	0.623258	5.3:-1598410	MS.gene024854:CDS
GGTCAGAAAGATGAAAAGGT+CGG	0.638104	5.3:+1598804	None:intergenic
TGTTGTGAAACAAGTTGTCA+AGG	0.644645	5.3:-1598516	MS.gene024854:CDS
TTTATTTCTTCCAAACCAGG+TGG	0.661410	5.3:-1598863	MS.gene024854:CDS
ATCTTGATTTCAGAGAAACA+CGG	0.706701	5.3:+1598624	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATACCAAACAAATTTCTA+TGG	-	chr5.3:1598518-1598537	MS.gene024854:CDS	20.0%
!	ATATTTAGATCCTAGAACAT+AGG	+	chr5.3:1598641-1598660	None:intergenic	25.0%
!	TTGTTGATGTTGAAAATGTT+GGG	-	chr5.3:1598749-1598768	MS.gene024854:CDS	25.0%
!	TTTGTTGATGTTGAAAATGT+TGG	-	chr5.3:1598748-1598767	MS.gene024854:CDS	25.0%
!!!	ATGTTCTAGGATCTAAATAT+TGG	-	chr5.3:1598641-1598660	MS.gene024854:CDS	25.0%
!!!	TGTTCTAGGATCTAAATATT+GGG	-	chr5.3:1598642-1598661	MS.gene024854:CDS	25.0%
	ATCTTGATTTCAGAGAAACA+CGG	+	chr5.3:1598579-1598598	None:intergenic	30.0%
!	TTGCACCATAGAAATTTGTT+TGG	+	chr5.3:1598526-1598545	None:intergenic	30.0%
!!	ACTTTTATTTCTTCCAAACC+AGG	-	chr5.3:1598334-1598353	MS.gene024854:CDS	30.0%
!!	AGGAAGTATTTGTTCATGAA+AGG	-	chr5.3:1598820-1598839	MS.gene024854:CDS	30.0%
!!	GTTCTAGGATCTAAATATTG+GGG	-	chr5.3:1598643-1598662	MS.gene024854:CDS	30.0%
	GGATCTAAATATTGGGGTAA+AGG	-	chr5.3:1598649-1598668	MS.gene024854:CDS	35.0%
	TCAAGGTCAGAAAGATGAAA+AGG	+	chr5.3:1598403-1598422	None:intergenic	35.0%
	TGTTGTGAAACAAGTTGTCA+AGG	-	chr5.3:1598684-1598703	MS.gene024854:CDS	35.0%
!	AACTTGTTTCACAACATACG+TGG	+	chr5.3:1598680-1598699	None:intergenic	35.0%
!	TTTATTTCTTCCAAACCAGG+TGG	-	chr5.3:1598337-1598356	MS.gene024854:CDS	35.0%
!!	TTGTTCCTGCTTTTATCATG+AGG	+	chr5.3:1598604-1598623	None:intergenic	35.0%
	ACAGAACTTGCCTATGTTCT+AGG	-	chr5.3:1598628-1598647	MS.gene024854:CDS	40.0%
	ATGGAGAGCTGATGAAAAAG+TGG	-	chr5.3:1598435-1598454	MS.gene024854:CDS	40.0%
	GATCACCTCATGATAAAAGC+AGG	-	chr5.3:1598596-1598615	MS.gene024854:CDS	40.0%
	GGTCAGAAAGATGAAAAGGT+CGG	+	chr5.3:1598399-1598418	None:intergenic	40.0%
	TCCAGAAGGAAGGAATTCTT+AGG	-	chr5.3:1598800-1598819	MS.gene024854:CDS	40.0%
	TCCTAAGAATTCCTTCCTTC+TGG	+	chr5.3:1598804-1598823	None:intergenic	40.0%
	TGCCTTAATGATCGTGCTAT+TGG	-	chr5.3:1598553-1598572	MS.gene024854:CDS	40.0%
	CCATACACCAGCAAAGAACA+AGG	-	chr5.3:1598478-1598497	MS.gene024854:CDS	45.0%
	CCTTGTTCTTTGCTGGTGTA+TGG	+	chr5.3:1598481-1598500	None:intergenic	45.0%
	GGCCAATAGCACGATCATTA+AGG	+	chr5.3:1598558-1598577	None:intergenic	45.0%
	TTTCATCAGCTCTCCATACC+AGG	+	chr5.3:1598432-1598451	None:intergenic	45.0%
!	AAAGCTGGTTTCCAGAAGGA+AGG	-	chr5.3:1598790-1598809	MS.gene024854:CDS	45.0%
!	ATGCTCTTTTCATTGCCACC+TGG	+	chr5.3:1598355-1598374	None:intergenic	45.0%
!	CTTTTCATTGCCACCTGGTT+TGG	+	chr5.3:1598350-1598369	None:intergenic	45.0%
!	GTTGATGCCTTGTTCTTTGC+TGG	+	chr5.3:1598488-1598507	None:intergenic	45.0%
!	TGTTGGGTCTCAAAGAGTGT+TGG	-	chr5.3:1598765-1598784	MS.gene024854:CDS	45.0%
!!	CAAAGAGTGTTGGAGAAAGC+TGG	-	chr5.3:1598775-1598794	MS.gene024854:CDS	45.0%
	AAGTGGCCAAGTTCTGCTCT+TGG	-	chr5.3:1598452-1598471	MS.gene024854:CDS	50.0%
	ACCTTGATGAGCTCCTGGTA+TGG	-	chr5.3:1598416-1598435	MS.gene024854:CDS	50.0%
	AGTGGCCAAGTTCTGCTCTT+GGG	-	chr5.3:1598453-1598472	MS.gene024854:CDS	50.0%
	GAAAAGGTCGGAGTGCGATT+TGG	+	chr5.3:1598387-1598406	None:intergenic	50.0%
	TCCATACCAGGAGCTCATCA+AGG	+	chr5.3:1598420-1598439	None:intergenic	50.0%
	TGGTTCCCAAGAGCAGAACT+TGG	+	chr5.3:1598461-1598480	None:intergenic	50.0%
	TTCTGACCTTGATGAGCTCC+TGG	-	chr5.3:1598411-1598430	MS.gene024854:CDS	50.0%
!	GGAGAAAGCTGGTTTCCAGA+AGG	-	chr5.3:1598786-1598805	MS.gene024854:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	1598325	1598897	1598325	ID=MS.gene024854
chr5.3	mRNA	1598325	1598897	1598325	ID=MS.gene024854.t1;Parent=MS.gene024854
chr5.3	exon	1598325	1598897	1598325	ID=MS.gene024854.t1.exon1;Parent=MS.gene024854.t1
chr5.3	CDS	1598325	1598897	1598325	ID=cds.MS.gene024854.t1;Parent=MS.gene024854.t1

Gene Sequence

>MS.gene024854

ATGGAGGAAACTTTTATTTCTTCCAAACCAGGTGGCAATGAAAAGAGCATTGATTTGAACCAAATCGCACTCCGACCTTTTCATCTTTCTGACCTTGATGAGCTCCTGGTATGGAGAGCTGATGAAAAAGTGGCCAAGTTCTGCTCTTGGGAACCATACACCAGCAAAGAACAAGGCATCAACTTCATTGAAAATATACCAAACAAATTTCTATGGTGCAAAGCAATATGCCTTAATGATCGTGCTATTGGCCGTGTTTCTCTGAAATCAAGATCACCTCATGATAAAAGCAGGAACAAAACTACAGAACTTGCCTATGTTCTAGGATCTAAATATTGGGGTAAAGGAATTGCCACGTATGTTGTGAAACAAGTTGTCAAGGTTGCATTCAGTGAGTTGTCACACTTAGAAAGAGTTGAAGCTTTTGTTGATGTTGAAAATGTTGGGTCTCAAAGAGTGTTGGAGAAAGCTGGTTTCCAGAAGGAAGGAATTCTTAGGAAGTATTTGTTCATGAAAGGAAAAAGTAGAGATATGATCATATTCAGTGTTCTTTCTAATGATCTCCAACTATGA

Protein sequence

>MS.gene024854.t1

MEETFISSKPGGNEKSIDLNQIALRPFHLSDLDELLVWRADEKVAKFCSWEPYTSKEQGINFIENIPNKFLWCKAICLNDRAIGRVSLKSRSPHDKSRNKTTELAYVLGSKYWGKGIATYVVKQVVKVAFSELSHLERVEAFVDVENVGSQRVLEKAGFQKEGILRKYLFMKGKSRDMIIFSVLSNDLQL