AlfalfaGEDB | MS.gene49472

M. sativa cultivar XinJiangDaYe / MS.gene49472

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49472.t1	XP_003610737.1	93.7	190	12	0	1	190	1	190	1.30E-93	352.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49472.t1	P05332	31.8	179	111	3	15	187	2	175	8.6e-14	78.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49472.t1	G7K3P2	93.7	190	12	0	1	190	1	190	9.2e-94	352.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene49472.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene34370	MS.gene49472	PPI
MS.gene49472	MS.gene21830	PPI
MS.gene49472	MS.gene34371	PPI
MS.gene49472	MS.gene001034	PPI
MS.gene49472	MS.gene070945	PPI
MS.gene49472	MS.gene21827	PPI
MS.gene49472	MS.gene071361	PPI
MS.gene49472	MS.gene34370	PPI
MS.gene49472	MS.gene043813	PPI
MS.gene49472	MS.gene21831	PPI
MS.gene043815	MS.gene49472	PPI
MS.gene49472	MS.gene77154	PPI
MS.gene49472	MS.gene49468	PPI
MS.gene29937	MS.gene49472	PPI
MS.gene49472	MS.gene006417	PPI
MS.gene024858	MS.gene49472	PPI
MS.gene024855	MS.gene49472	PPI
MS.gene49472	MS.gene024857	PPI
MS.gene49472	MS.gene070944	PPI
MS.gene49472	MS.gene95801	PPI
MS.gene49472	MS.gene49471	PPI
MS.gene49472	MS.gene49470	PPI
MS.gene49472	MS.gene024614	PPI
MS.gene49472	MS.gene024859	PPI
MS.gene49472	MS.gene95802	PPI
MS.gene49472	MS.gene001032	PPI
MS.gene49472	MS.gene74082	PPI
MS.gene49472	MS.gene006418	PPI
MS.gene49472	MS.gene001037	PPI
MS.gene49472	MS.gene21833	PPI
MS.gene49472	MS.gene001038	PPI
MS.gene49472	MS.gene071357	PPI
MS.gene49472	MS.gene050493	PPI
MS.gene49472	MS.gene21828	PPI
MS.gene49472	MS.gene001033	PPI
MS.gene49472	MS.gene024854	PPI
MS.gene49472	MS.gene22888	PPI
MS.gene49472	MS.gene04530	PPI
MS.gene49472	MS.gene024612	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49472.t1	MTR_5g006450	93.684	190	12	0	1	190	1	190	3.14e-129	361
MS.gene49472.t1	MTR_5g006470	76.842	190	42	1	1	190	1	188	1.11e-101	291
MS.gene49472.t1	MTR_5g006520	77.895	190	42	0	1	190	1719	1908	7.35e-98	310
MS.gene49472.t1	MTR_5g006520	52.000	75	13	1	97	171	1655	1706	2.64e-12	65.1
MS.gene49472.t1	MTR_5g006510	73.298	191	44	3	1	190	7	191	1.05e-94	273
MS.gene49472.t1	MTR_5g006460	73.333	195	45	5	1	190	1	193	1.77e-93	270
MS.gene49472.t1	MTR_1g103110	64.583	192	64	2	1	190	1	190	2.20e-85	250
MS.gene49472.t1	MTR_1g103150	60.417	192	72	2	1	190	1	190	2.37e-79	234
MS.gene49472.t1	MTR_7g114410	41.279	172	98	1	16	187	3	171	1.35e-45	148
MS.gene49472.t1	MTR_3g463610	39.766	171	103	0	17	187	3	173	1.64e-44	147
MS.gene49472.t1	MTR_7g114410	40.764	157	90	1	16	172	3	156	1.45e-41	137
MS.gene49472.t1	MTR_7g024760	41.071	168	94	3	20	187	7	169	2.46e-37	127
MS.gene49472.t1	MTR_7g024780	39.181	171	100	2	17	187	4	170	1.18e-36	125
MS.gene49472.t1	MTR_7g024765	38.690	168	98	3	20	187	7	169	1.30e-35	123
MS.gene49472.t1	MTR_1g103320	55.128	78	33	1	53	130	13	88	2.15e-20	82.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49472.t1	AT2G32030	51.786	168	74	3	21	187	24	185	1.60e-58	182
MS.gene49472.t1	AT2G32020	51.190	168	75	2	21	187	19	180	2.37e-54	171
MS.gene49472.t1	AT3G22560	38.235	170	100	2	20	187	6	172	2.44e-35	122

Find 49 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAAATCAATTCTCTCTTCTT+TGG	0.250774	5.1:+1234381	None:intergenic
ATGTTCTAGGCTCTAAATAT+TGG	0.292142	5.1:-1234099	MS.gene49472:CDS
TCCTTAGAACTCCCTCCTTT+TGG	0.297796	5.1:+1233939	None:intergenic
GCAGAACTTGGCTATGTTCT+AGG	0.302641	5.1:-1234112	MS.gene49472:CDS
TGTTCTAGGCTCTAAATATT+GGG	0.316974	5.1:-1234098	MS.gene49472:CDS
GAGTTCCCAAGAGCAGAATT+TGG	0.329740	5.1:+1234282	None:intergenic
GAAGGGCTCGAAGTGTGATT+TGG	0.338927	5.1:+1234356	None:intergenic
GCTCTAAATATTGGGGTAAA+GGG	0.356917	5.1:-1234090	MS.gene49472:CDS
AAGTTCTGCACATTTGTTCC+TGG	0.375848	5.1:+1234126	None:intergenic
CTAAACAATTGAATTATAGT+TGG	0.392086	5.1:+1233852	None:intergenic
ATATAGCTACCAAGTTTCTA+TGG	0.402105	5.1:-1234222	MS.gene49472:CDS
TGCATCAATGATCGTGCAAT+TGG	0.403589	5.1:-1234187	MS.gene49472:CDS
AAGGGCTCGAAGTGTGATTT+GGG	0.403990	5.1:+1234357	None:intergenic
TCCAAAAGGAGGGAGTTCTA+AGG	0.410439	5.1:-1233940	MS.gene49472:CDS
GGCTCTAAATATTGGGGTAA+AGG	0.422886	5.1:-1234091	MS.gene49472:CDS
AAGTGGCCAAATTCTGCTCT+TGG	0.423997	5.1:-1234288	MS.gene49472:CDS
GTTGATGCCATCATCCTTGC+TGG	0.429573	5.1:+1234255	None:intergenic
TGCTGGGTCTCAAAGAGTGC+TGG	0.460731	5.1:-1233975	MS.gene49472:CDS
AGTGGCCAAATTCTGCTCTT+GGG	0.474265	5.1:-1234287	MS.gene49472:CDS
AGGAACAAATGTGCAGAACT+TGG	0.475881	5.1:-1234124	MS.gene49472:CDS
AGGAAATATTTGTTCATGAA+AGG	0.480337	5.1:-1233920	MS.gene49472:CDS
GCTTGAAGCTTTAGTTGATG+TGG	0.488968	5.1:-1234002	MS.gene49472:CDS
TGCTTTGCACCATAGAAACT+TGG	0.496119	5.1:+1234213	None:intergenic
TTCTGACCTTGATGATCTCA+TGG	0.505164	5.1:-1234329	MS.gene49472:CDS
CATCAAGGTCAGAAAGTTGA+AGG	0.505953	5.1:+1234338	None:intergenic
TGTTGTGAAACAAGTTGTTA+AGG	0.509712	5.1:-1234056	MS.gene49472:CDS
TTGTTCCTGGATTTATCACC+AGG	0.514781	5.1:+1234139	None:intergenic
ATTCTGTGAGTTGACTTACT+TGG	0.516104	5.1:-1234029	MS.gene49472:CDS
CAAAGAGTGCTGGAGAAAGC+TGG	0.517457	5.1:-1233965	MS.gene49472:CDS
TTAGTTGATGTGGAAAATGC+TGG	0.527178	5.1:-1233992	MS.gene49472:CDS
GTGAGTTGACTTACTTGGAA+AGG	0.541309	5.1:-1234024	MS.gene49472:CDS
GTTCACCTGGTGATAAATCC+AGG	0.542743	5.1:-1234144	MS.gene49472:CDS
TCTCTATCCTCAAGTTCACC+TGG	0.545696	5.1:-1234157	MS.gene49472:CDS
CTCTACACCAGCAAGGATGA+TGG	0.559987	5.1:-1234262	MS.gene49472:CDS
GAAAGCTGGTTTCCAAAAGG+AGG	0.562242	5.1:-1233951	MS.gene49472:CDS
CTCTAAATATTGGGGTAAAG+GGG	0.564900	5.1:-1234089	MS.gene49472:CDS
GCATCAATGATCGTGCAATT+GGG	0.567462	5.1:-1234186	MS.gene49472:CDS
GGAGAAAGCTGGTTTCCAAA+AGG	0.570344	5.1:-1233954	MS.gene49472:CDS
TTTATCACCAGGTGAACTTG+AGG	0.573291	5.1:+1234150	None:intergenic
TAGTTGATGTGGAAAATGCT+GGG	0.573363	5.1:-1233991	MS.gene49472:CDS
ATTCTCTCTTCTTTGGCACG+TGG	0.591899	5.1:+1234388	None:intergenic
ACCTTGATGATCTCATGGTG+TGG	0.606125	5.1:-1234324	MS.gene49472:CDS
AACTTGTTTCACAACACATG+TGG	0.607173	5.1:+1234063	None:intergenic
GTTCTAGGCTCTAAATATTG+GGG	0.619040	5.1:-1234097	MS.gene49472:CDS
TCCACACCATGAGATCATCA+AGG	0.646997	5.1:+1234323	None:intergenic
ATCAAGGTCAGAAAGTTGAA+GGG	0.653293	5.1:+1234339	None:intergenic
AAAGCTGGTTTCCAAAAGGA+GGG	0.657724	5.1:-1233950	MS.gene49472:CDS
GAGATCAGTAGAAAGAACAC+TGG	0.658551	5.1:+1233874	None:intergenic
TTGGGAACTCTACACCAGCA+AGG	0.679749	5.1:-1234269	MS.gene49472:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGGAAATATTTGTTCATGAA+AGG	-	chr5.1:1234360-1234379	MS.gene49472:CDS	25.0%
!	TAAATCAATTCTCTCTTCTT+TGG	+	chr5.1:1233902-1233921	None:intergenic	25.0%
	ATATAGCTACCAAGTTTCTA+TGG	-	chr5.1:1234058-1234077	MS.gene49472:CDS	30.0%
	TGTTGTGAAACAAGTTGTTA+AGG	-	chr5.1:1234224-1234243	MS.gene49472:CDS	30.0%
!!	ATGTTCTAGGCTCTAAATAT+TGG	-	chr5.1:1234181-1234200	MS.gene49472:CDS	30.0%
!!	TGTTCTAGGCTCTAAATATT+GGG	-	chr5.1:1234182-1234201	MS.gene49472:CDS	30.0%
	ATCAAGGTCAGAAAGTTGAA+GGG	+	chr5.1:1233944-1233963	None:intergenic	35.0%
	CTCTAAATATTGGGGTAAAG+GGG	-	chr5.1:1234191-1234210	MS.gene49472:CDS	35.0%
!	AACTTGTTTCACAACACATG+TGG	+	chr5.1:1234220-1234239	None:intergenic	35.0%
!	ATTCTGTGAGTTGACTTACT+TGG	-	chr5.1:1234251-1234270	MS.gene49472:CDS	35.0%
!	GCTCTAAATATTGGGGTAAA+GGG	-	chr5.1:1234190-1234209	MS.gene49472:CDS	35.0%
!	TAGTTGATGTGGAAAATGCT+GGG	-	chr5.1:1234289-1234308	MS.gene49472:CDS	35.0%
!	TTAGTTGATGTGGAAAATGC+TGG	-	chr5.1:1234288-1234307	MS.gene49472:CDS	35.0%
!!	GTTCTAGGCTCTAAATATTG+GGG	-	chr5.1:1234183-1234202	MS.gene49472:CDS	35.0%
	AAGTTCTGCACATTTGTTCC+TGG	+	chr5.1:1234157-1234176	None:intergenic	40.0%
	AGGAACAAATGTGCAGAACT+TGG	-	chr5.1:1234156-1234175	MS.gene49472:CDS	40.0%
	CATCAAGGTCAGAAAGTTGA+AGG	+	chr5.1:1233945-1233964	None:intergenic	40.0%
	GAGATCAGTAGAAAGAACAC+TGG	+	chr5.1:1234409-1234428	None:intergenic	40.0%
	GCATCAATGATCGTGCAATT+GGG	-	chr5.1:1234094-1234113	MS.gene49472:CDS	40.0%
	GTGGACAACTGATGAAAAAG+TGG	-	chr5.1:1233975-1233994	MS.gene49472:CDS	40.0%
	TGCATCAATGATCGTGCAAT+TGG	-	chr5.1:1234093-1234112	MS.gene49472:CDS	40.0%
	TGCTTTGCACCATAGAAACT+TGG	+	chr5.1:1234070-1234089	None:intergenic	40.0%
	TTGTTCCTGGATTTATCACC+AGG	+	chr5.1:1234144-1234163	None:intergenic	40.0%
	TTTATCACCAGGTGAACTTG+AGG	+	chr5.1:1234133-1234152	None:intergenic	40.0%
!	AAAGCTGGTTTCCAAAAGGA+GGG	-	chr5.1:1234330-1234349	MS.gene49472:CDS	40.0%
!	GCTTGAAGCTTTAGTTGATG+TGG	-	chr5.1:1234278-1234297	MS.gene49472:CDS	40.0%
!	GTGAGTTGACTTACTTGGAA+AGG	-	chr5.1:1234256-1234275	MS.gene49472:CDS	40.0%
!	TTCTGACCTTGATGATCTCA+TGG	-	chr5.1:1233951-1233970	MS.gene49472:CDS	40.0%
!!	GGCTCTAAATATTGGGGTAA+AGG	-	chr5.1:1234189-1234208	MS.gene49472:CDS	40.0%
	AAGGGCTCGAAGTGTGATTT+GGG	+	chr5.1:1233926-1233945	None:intergenic	45.0%
	AAGTGGCCAAATTCTGCTCT+TGG	-	chr5.1:1233992-1234011	MS.gene49472:CDS	45.0%
	AGTGGCCAAATTCTGCTCTT+GGG	-	chr5.1:1233993-1234012	MS.gene49472:CDS	45.0%
	ATTCTCTCTTCTTTGGCACG+TGG	+	chr5.1:1233895-1233914	None:intergenic	45.0%
	GAGTTCCCAAGAGCAGAATT+TGG	+	chr5.1:1234001-1234020	None:intergenic	45.0%
	GCAGAACTTGGCTATGTTCT+AGG	-	chr5.1:1234168-1234187	MS.gene49472:CDS	45.0%
	GTTCACCTGGTGATAAATCC+AGG	-	chr5.1:1234136-1234155	MS.gene49472:CDS	45.0%
	TCCAAAAGGAGGGAGTTCTA+AGG	-	chr5.1:1234340-1234359	MS.gene49472:CDS	45.0%
	TCCACACCATGAGATCATCA+AGG	+	chr5.1:1233960-1233979	None:intergenic	45.0%
	TCTCTATCCTCAAGTTCACC+TGG	-	chr5.1:1234123-1234142	MS.gene49472:CDS	45.0%
!	ACCTTGATGATCTCATGGTG+TGG	-	chr5.1:1233956-1233975	MS.gene49472:CDS	45.0%
!	GAAAGCTGGTTTCCAAAAGG+AGG	-	chr5.1:1234329-1234348	MS.gene49472:CDS	45.0%
!	GGAGAAAGCTGGTTTCCAAA+AGG	-	chr5.1:1234326-1234345	MS.gene49472:CDS	45.0%
!	TCCTTAGAACTCCCTCCTTT+TGG	+	chr5.1:1234344-1234363	None:intergenic	45.0%
	CTCTACACCAGCAAGGATGA+TGG	-	chr5.1:1234018-1234037	MS.gene49472:CDS	50.0%
	GAAGGGCTCGAAGTGTGATT+TGG	+	chr5.1:1233927-1233946	None:intergenic	50.0%
	GTTGATGCCATCATCCTTGC+TGG	+	chr5.1:1234028-1234047	None:intergenic	50.0%
	TTGGGAACTCTACACCAGCA+AGG	-	chr5.1:1234011-1234030	MS.gene49472:CDS	50.0%
!!	CAAAGAGTGCTGGAGAAAGC+TGG	-	chr5.1:1234315-1234334	MS.gene49472:CDS	50.0%
!	TGCTGGGTCTCAAAGAGTGC+TGG	-	chr5.1:1234305-1234324	MS.gene49472:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	1233865	1234437	1233865	ID=MS.gene49472
chr5.1	mRNA	1233865	1234437	1233865	ID=MS.gene49472.t1;Parent=MS.gene49472
chr5.1	exon	1233865	1234437	1233865	ID=MS.gene49472.t1.exon1;Parent=MS.gene49472.t1
chr5.1	CDS	1233865	1234437	1233865	ID=cds.MS.gene49472.t1;Parent=MS.gene49472.t1

Gene Sequence

>MS.gene49472

ATGGAGAGAACTTCTATTTCTTTCGAACCACGTGCCAAAGAAGAGAGAATTGATTTAACCCAAATCACACTTCGAGCCCTTCAACTTTCTGACCTTGATGATCTCATGGTGTGGACAACTGATGAAAAAGTGGCCAAATTCTGCTCTTGGGAACTCTACACCAGCAAGGATGATGGCATCAACTTCATTGAAAATATAGCTACCAAGTTTCTATGGTGCAAAGCAATATGCATCAATGATCGTGCAATTGGGTGTGTTTCTCTATCCTCAAGTTCACCTGGTGATAAATCCAGGAACAAATGTGCAGAACTTGGCTATGTTCTAGGCTCTAAATATTGGGGTAAAGGGGTTGCCACATGTGTTGTGAAACAAGTTGTTAAGGTTGCATTCTGTGAGTTGACTTACTTGGAAAGGCTTGAAGCTTTAGTTGATGTGGAAAATGCTGGGTCTCAAAGAGTGCTGGAGAAAGCTGGTTTCCAAAAGGAGGGAGTTCTAAGGAAATATTTGTTCATGAAAGGAAAAAGTAGAGATATGATCATGTCCAGTGTTCTTTCTACTGATCTCCAACTATAA

Protein sequence

>MS.gene49472.t1

MERTSISFEPRAKEERIDLTQITLRALQLSDLDDLMVWTTDEKVAKFCSWELYTSKDDGINFIENIATKFLWCKAICINDRAIGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVATCVVKQVVKVAFCELTYLERLEALVDVENAGSQRVLEKAGFQKEGVLRKYLFMKGKSRDMIMSSVLSTDLQL