AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070944


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070944.t1 MTR_1g103150 96.757 185 6 0 1 185 1 185 7.81e-133 370
MS.gene070944.t1 MTR_1g103110 84.865 185 28 0 1 185 1 185 4.55e-114 322
MS.gene070944.t1 MTR_5g006470 62.766 188 68 1 1 188 1 186 3.17e-81 239
MS.gene070944.t1 MTR_5g006450 62.903 186 65 2 1 184 1 184 1.13e-80 238
MS.gene070944.t1 MTR_5g006520 60.000 190 72 2 1 188 1719 1906 4.18e-72 236
MS.gene070944.t1 MTR_5g006460 58.763 194 71 6 1 188 1 191 1.35e-68 207
MS.gene070944.t1 MTR_5g006510 52.356 191 86 3 1 188 1 189 8.31e-67 202
MS.gene070944.t1 MTR_7g114410 48.521 169 82 2 19 185 4 169 1.25e-48 156
MS.gene070944.t1 MTR_3g463610 44.186 172 88 2 19 185 3 171 7.05e-46 150
MS.gene070944.t1 MTR_7g114410 48.718 156 75 2 19 172 4 156 3.60e-44 144
MS.gene070944.t1 MTR_7g024765 42.683 164 91 3 22 185 7 167 4.77e-38 129
MS.gene070944.t1 MTR_7g024760 42.424 165 92 3 22 186 7 168 1.14e-37 128
MS.gene070944.t1 MTR_7g024780 39.881 168 99 2 17 184 2 167 2.33e-37 127
MS.gene070944.t1 MTR_1g103320 57.282 103 43 1 53 154 11 113 1.30e-33 116
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070944.t1 AT2G32020 52.727 165 73 3 22 185 18 178 1.25e-54 172
MS.gene070944.t1 AT2G32030 46.196 184 94 3 3 185 4 183 2.12e-54 171
MS.gene070944.t1 AT3G22560 41.420 169 92 2 18 182 2 167 4.51e-36 124

Find 47 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAAAATATCTGTTCATTA+AGG 0.174922 1.4:-79769323 MS.gene070944:CDS
TGTGGTTGAAGCTGCATTTA+AGG 0.195666 1.4:-79769446 MS.gene070944:CDS
TTCTTTGAGCTCCATGTATT+GGG 0.213171 1.4:-79769500 MS.gene070944:CDS
CTCTTCTTTAGCATCTGATT+TGG 0.248198 1.4:+79769795 None:intergenic
ATTCTTTGAGCTCCATGTAT+TGG 0.293943 1.4:-79769501 MS.gene070944:CDS
GCCTCAAGCCTCTCCAAGTA+TGG 0.329009 1.4:+79769418 None:intergenic
GAGATTTGCTTGCTATGTTT+TGG 0.329625 1.4:+79769623 None:intergenic
CCTTATACAAACAAAGAAAA+AGG 0.343403 1.4:-79769658 MS.gene070944:CDS
TGCCTTCGCGATAGAGCAAT+TGG 0.346097 1.4:-79769583 MS.gene070944:CDS
GGGCAAAGGGATTGCCACTA+TGG 0.352044 1.4:-79769479 MS.gene070944:CDS
TCAAGATCAGAAAGACTAAT+TGG 0.352059 1.4:+79769736 None:intergenic
TTTAGCATCTGATTTGGAAT+AGG 0.367598 1.4:+79769801 None:intergenic
AATTGGGTGTACCGAGTTCC+AGG 0.375481 1.4:-79769566 MS.gene070944:CDS
AGAAAATATCTGTTCATTAA+GGG 0.384110 1.4:-79769322 MS.gene070944:CDS
ATTTAAGGAGTTTCCATACT+TGG 0.408342 1.4:-79769431 MS.gene070944:CDS
ACATAGCAAGCAAATCTCTA+TGG 0.412043 1.4:-79769618 MS.gene070944:CDS
CAAAGGGTGTTGGAGAAGGC+TGG 0.437360 1.4:-79769367 MS.gene070944:CDS
TACCGAGTTCCAGGTGTGTT+CGG 0.452367 1.4:-79769557 MS.gene070944:CDS
TAATTGGTCGAAGAGAGATT+TGG 0.457252 1.4:+79769752 None:intergenic
CGGGACAAATCTGCTCAACT+TGG 0.459831 1.4:-79769526 MS.gene070944:CDS
GGCATCTATCCGAACACACC+TGG 0.486937 1.4:+79769548 None:intergenic
AGCTCCATGTATTGGGGCAA+AGG 0.494926 1.4:-79769493 MS.gene070944:CDS
AATCCCTTTGCCCCAATACA+TGG 0.496567 1.4:+79769489 None:intergenic
AGGTGTGTTCGGATAGATGC+CGG 0.506000 1.4:-79769546 MS.gene070944:CDS
TTCTCAAAGGGTGTTGGAGA+AGG 0.511828 1.4:-79769371 MS.gene070944:CDS
GCCTTCGCGATAGAGCAATT+GGG 0.515394 1.4:-79769582 MS.gene070944:CDS
GCTCCATGTATTGGGGCAAA+GGG 0.517436 1.4:-79769492 MS.gene070944:CDS
ATCTTGATGATGTTATGTTG+TGG 0.524636 1.4:-79769720 MS.gene070944:CDS
AGGTTCCCAAGTGCAATACT+TGG 0.527166 1.4:+79769678 None:intergenic
CAAGTTGAGCAGATTTGTCC+CGG 0.527345 1.4:+79769527 None:intergenic
GAAAATATCTGTTCATTAAG+GGG 0.536787 1.4:-79769321 MS.gene070944:CDS
TGTGGCTTCTCAAAGGGTGT+TGG 0.546349 1.4:-79769377 MS.gene070944:CDS
TCCATACTTGGAGAGGCTTG+AGG 0.557426 1.4:-79769419 MS.gene070944:CDS
CACATGTTTCACAACCATAG+TGG 0.561077 1.4:+79769465 None:intergenic
GCTTGAGGCTCGAGTTGATG+TGG 0.569120 1.4:-79769404 MS.gene070944:CDS
TGGAAAATGTGGCTTCTCAA+AGG 0.572207 1.4:-79769384 MS.gene070944:CDS
AGGAGTTTCCATACTTGGAG+AGG 0.574247 1.4:-79769426 MS.gene070944:CDS
ATCCGAACACACCTGGAACT+CGG 0.577234 1.4:+79769555 None:intergenic
TCTTTGAGCTCCATGTATTG+GGG 0.587210 1.4:-79769499 MS.gene070944:CDS
GGAAAATGTGGCTTCTCAAA+GGG 0.588500 1.4:-79769383 MS.gene070944:CDS
AAGTGGCCAAGTATTGCACT+TGG 0.595225 1.4:-79769684 MS.gene070944:CDS
GGTGTGTTCGGATAGATGCC+GGG 0.599178 1.4:-79769545 MS.gene070944:CDS
GTGGACTAGTGATGCAAAAG+TGG 0.603948 1.4:-79769701 MS.gene070944:CDS
ACCCAATTGCTCTATCGCGA+AGG 0.616201 1.4:+79769581 None:intergenic
TCGAGTTGATGTGGAAAATG+TGG 0.669414 1.4:-79769395 MS.gene070944:CDS
AGTGGCCAAGTATTGCACTT+GGG 0.670287 1.4:-79769683 MS.gene070944:CDS
CACTATGGTTGTGAAACATG+TGG 0.680456 1.4:-79769464 MS.gene070944:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAATATCTGTTCATTAA+GGG - chr1.4:79769768-79769787 MS.gene070944:CDS 20.0%
! CAGAAAATATCTGTTCATTA+AGG - chr1.4:79769767-79769786 MS.gene070944:CDS 25.0%
! CCTTATACAAACAAAGAAAA+AGG - chr1.4:79769432-79769451 MS.gene070944:CDS 25.0%
! GAAAATATCTGTTCATTAAG+GGG - chr1.4:79769769-79769788 MS.gene070944:CDS 25.0%
!!! CCTTTTTCTTTGTTTGTATA+AGG + chr1.4:79769435-79769454 None:intergenic 25.0%
ATTTAAGGAGTTTCCATACT+TGG - chr1.4:79769659-79769678 MS.gene070944:CDS 30.0%
TCAAGATCAGAAAGACTAAT+TGG + chr1.4:79769357-79769376 None:intergenic 30.0%
!! ATCTTGATGATGTTATGTTG+TGG - chr1.4:79769370-79769389 MS.gene070944:CDS 30.0%
!! TTTAGCATCTGATTTGGAAT+AGG + chr1.4:79769292-79769311 None:intergenic 30.0%
ACATAGCAAGCAAATCTCTA+TGG - chr1.4:79769472-79769491 MS.gene070944:CDS 35.0%
ATTCTTTGAGCTCCATGTAT+TGG - chr1.4:79769589-79769608 MS.gene070944:CDS 35.0%
CTCTTCTTTAGCATCTGATT+TGG + chr1.4:79769298-79769317 None:intergenic 35.0%
TAATTGGTCGAAGAGAGATT+TGG + chr1.4:79769341-79769360 None:intergenic 35.0%
TTCTTTGAGCTCCATGTATT+GGG - chr1.4:79769590-79769609 MS.gene070944:CDS 35.0%
!!! GAGATTTGCTTGCTATGTTT+TGG + chr1.4:79769470-79769489 None:intergenic 35.0%
CACATGTTTCACAACCATAG+TGG + chr1.4:79769628-79769647 None:intergenic 40.0%
CACTATGGTTGTGAAACATG+TGG - chr1.4:79769626-79769645 MS.gene070944:CDS 40.0%
GGAAAATGTGGCTTCTCAAA+GGG - chr1.4:79769707-79769726 MS.gene070944:CDS 40.0%
TCTTTGAGCTCCATGTATTG+GGG - chr1.4:79769591-79769610 MS.gene070944:CDS 40.0%
TGGAAAATGTGGCTTCTCAA+AGG - chr1.4:79769706-79769725 MS.gene070944:CDS 40.0%
TGTGGTTGAAGCTGCATTTA+AGG - chr1.4:79769644-79769663 MS.gene070944:CDS 40.0%
!! TCGAGTTGATGTGGAAAATG+TGG - chr1.4:79769695-79769714 MS.gene070944:CDS 40.0%
AATCCCTTTGCCCCAATACA+TGG + chr1.4:79769604-79769623 None:intergenic 45.0%
AGGAGTTTCCATACTTGGAG+AGG - chr1.4:79769664-79769683 MS.gene070944:CDS 45.0%
AGGTTCCCAAGTGCAATACT+TGG + chr1.4:79769415-79769434 None:intergenic 45.0%
! AAGTGGCCAAGTATTGCACT+TGG - chr1.4:79769406-79769425 MS.gene070944:CDS 45.0%
! AGTGGCCAAGTATTGCACTT+GGG - chr1.4:79769407-79769426 MS.gene070944:CDS 45.0%
! CAAGTTGAGCAGATTTGTCC+CGG + chr1.4:79769566-79769585 None:intergenic 45.0%
!! GTGGACTAGTGATGCAAAAG+TGG - chr1.4:79769389-79769408 MS.gene070944:CDS 45.0%
!! TTCTCAAAGGGTGTTGGAGA+AGG - chr1.4:79769719-79769738 MS.gene070944:CDS 45.0%
!!! AAGGCTGGTTTTGAAAGGGA+AGG - chr1.4:79769738-79769757 MS.gene070944:CDS 45.0%
!!! GGAGAAGGCTGGTTTTGAAA+GGG - chr1.4:79769734-79769753 MS.gene070944:CDS 45.0%
!!! TGGAGAAGGCTGGTTTTGAA+AGG - chr1.4:79769733-79769752 MS.gene070944:CDS 45.0%
AATTGGGTGTACCGAGTTCC+AGG - chr1.4:79769524-79769543 MS.gene070944:CDS 50.0%
ACCCAATTGCTCTATCGCGA+AGG + chr1.4:79769512-79769531 None:intergenic 50.0%
AGCTCCATGTATTGGGGCAA+AGG - chr1.4:79769597-79769616 MS.gene070944:CDS 50.0%
AGGTGTGTTCGGATAGATGC+CGG - chr1.4:79769544-79769563 MS.gene070944:CDS 50.0%
ATCCGAACACACCTGGAACT+CGG + chr1.4:79769538-79769557 None:intergenic 50.0%
CGGGACAAATCTGCTCAACT+TGG - chr1.4:79769564-79769583 MS.gene070944:CDS 50.0%
GCCTTCGCGATAGAGCAATT+GGG - chr1.4:79769508-79769527 MS.gene070944:CDS 50.0%
GCTCCATGTATTGGGGCAAA+GGG - chr1.4:79769598-79769617 MS.gene070944:CDS 50.0%
TACCGAGTTCCAGGTGTGTT+CGG - chr1.4:79769533-79769552 MS.gene070944:CDS 50.0%
TCCATACTTGGAGAGGCTTG+AGG - chr1.4:79769671-79769690 MS.gene070944:CDS 50.0%
TGCCTTCGCGATAGAGCAAT+TGG - chr1.4:79769507-79769526 MS.gene070944:CDS 50.0%
! TGTGGCTTCTCAAAGGGTGT+TGG - chr1.4:79769713-79769732 MS.gene070944:CDS 50.0%
GCCTCAAGCCTCTCCAAGTA+TGG + chr1.4:79769675-79769694 None:intergenic 55.0%
GGCATCTATCCGAACACACC+TGG + chr1.4:79769545-79769564 None:intergenic 55.0%
GGGCAAAGGGATTGCCACTA+TGG - chr1.4:79769611-79769630 MS.gene070944:CDS 55.0%
GGTGTGTTCGGATAGATGCC+GGG - chr1.4:79769545-79769564 MS.gene070944:CDS 55.0%
!! CAAAGGGTGTTGGAGAAGGC+TGG - chr1.4:79769723-79769742 MS.gene070944:CDS 55.0%
!! GCTTGAGGCTCGAGTTGATG+TGG - chr1.4:79769686-79769705 MS.gene070944:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 79769273 79769839 79769273 ID=MS.gene070944
chr1.4 mRNA 79769273 79769839 79769273 ID=MS.gene070944.t1;Parent=MS.gene070944
chr1.4 exon 79769273 79769839 79769273 ID=MS.gene070944.t1.exon1;Parent=MS.gene070944.t1
chr1.4 CDS 79769273 79769839 79769273 ID=cds.MS.gene070944.t1;Parent=MS.gene070944.t1
Gene Sequence

>MS.gene070944

ATGGAAGAAACTGCTACCTATTCCAAATCAGATGCTAAAGAAGAGTATTCTGTTGTTGACTTGAGCCAAATCTCTCTTCGACCAATTAGTCTTTCTGATCTTGATGATGTTATGTTGTGGACTAGTGATGCAAAAGTGGCCAAGTATTGCACTTGGGAACCTTATACAAACAAAGAAAAAGGCATTGACTTCATCCAAAACATAGCAAGCAAATCTCTATGGTTCAGAGCAATTTGCCTTCGCGATAGAGCAATTGGGTGTACCGAGTTCCAGGTGTGTTCGGATAGATGCCGGGACAAATCTGCTCAACTTGGCTATTCTTTGAGCTCCATGTATTGGGGCAAAGGGATTGCCACTATGGTTGTGAAACATGTGGTTGAAGCTGCATTTAAGGAGTTTCCATACTTGGAGAGGCTTGAGGCTCGAGTTGATGTGGAAAATGTGGCTTCTCAAAGGGTGTTGGAGAAGGCTGGTTTTGAAAGGGAAGGTGTTCTCAGAAAATATCTGTTCATTAAGGGGAAAAGTATAGATATTGTTATGTTCAGTATTCTCTCAGTGAAGTTATAA

Protein sequence

>MS.gene070944.t1

MEETATYSKSDAKEEYSVVDLSQISLRPISLSDLDDVMLWTSDAKVAKYCTWEPYTNKEKGIDFIQNIASKSLWFRAICLRDRAIGCTEFQVCSDRCRDKSAQLGYSLSSMYWGKGIATMVVKHVVEAAFKEFPYLERLEARVDVENVASQRVLEKAGFEREGVLRKYLFIKGKSIDIVMFSILSVKL