AlfalfaGEDB | MS.gene34370

M. sativa cultivar XinJiangDaYe / MS.gene34370

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34370.t1	XP_013469781.1	95.8	190	8	0	1	190	1	190	4.20E-97	364

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34370.t1	P05332	34.5	171	103	3	23	187	8	175	1.0e-19	98.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34370.t1	A0A072VP71	95.8	190	8	0	1	190	1	190	3.1e-97	364.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene34370.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene34370	MS.gene21830	PPI
MS.gene34370	MS.gene34371	PPI
MS.gene34370	MS.gene001034	PPI
MS.gene34370	MS.gene49472	PPI
MS.gene34370	MS.gene21827	PPI
MS.gene34370	MS.gene070944	PPI
MS.gene34370	MS.gene071361	PPI
MS.gene49472	MS.gene34370	PPI
MS.gene34370	MS.gene21831	PPI
MS.gene34370	MS.gene49470	PPI
MS.gene34370	MS.gene77154	PPI
MS.gene34370	MS.gene49468	PPI
MS.gene34370	MS.gene006417	PPI
MS.gene34370	MS.gene024857	PPI
MS.gene043815	MS.gene34370	PPI
MS.gene34370	MS.gene95801	PPI
MS.gene34370	MS.gene001037	PPI
MS.gene29937	MS.gene34370	PPI
MS.gene024858	MS.gene34370	PPI
MS.gene34370	MS.gene21833	PPI
MS.gene024855	MS.gene34370	PPI
MS.gene34370	MS.gene024859	PPI
MS.gene34370	MS.gene95802	PPI
MS.gene34370	MS.gene001032	PPI
MS.gene34370	MS.gene74082	PPI
MS.gene34370	MS.gene49471	PPI
MS.gene34370	MS.gene006418	PPI
MS.gene34370	MS.gene001038	PPI
MS.gene34370	MS.gene071357	PPI
MS.gene34370	MS.gene050493	PPI
MS.gene34370	MS.gene21828	PPI
MS.gene34370	MS.gene070945	PPI
MS.gene34370	MS.gene001033	PPI
MS.gene34370	MS.gene024854	PPI
MS.gene34370	MS.gene22888	PPI
MS.gene34370	MS.gene04530	PPI
MS.gene34370	MS.gene043813	PPI
MS.gene34370	MS.gene024614	PPI
MS.gene34370	MS.gene024612	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34370.t1	MTR_1g103150	95.789	190	8	0	1	190	1	190	1.92e-134	374
MS.gene34370.t1	MTR_1g103110	82.632	190	33	0	1	190	1	190	1.70e-115	326
MS.gene34370.t1	MTR_5g006470	62.632	190	69	1	1	190	1	188	3.68e-83	244
MS.gene34370.t1	MTR_5g006450	61.458	192	70	2	1	190	1	190	1.28e-81	240
MS.gene34370.t1	MTR_5g006520	59.896	192	73	2	1	190	1719	1908	1.28e-73	241
MS.gene34370.t1	MTR_5g006460	58.163	196	73	6	1	190	1	193	1.15e-70	213
MS.gene34370.t1	MTR_5g006510	52.332	193	87	3	1	190	1	191	1.87e-68	207
MS.gene34370.t1	MTR_7g114410	47.368	171	85	2	19	187	4	171	1.71e-49	158
MS.gene34370.t1	MTR_3g463610	43.750	176	91	2	19	189	3	175	4.03e-46	151
MS.gene34370.t1	MTR_7g114410	48.077	156	76	2	19	172	4	156	1.88e-44	145
MS.gene34370.t1	MTR_7g024760	42.169	166	93	2	22	187	7	169	1.60e-39	133
MS.gene34370.t1	MTR_7g024765	42.169	166	93	3	22	187	7	169	1.42e-38	130
MS.gene34370.t1	MTR_7g024780	39.181	171	102	2	17	187	2	170	4.21e-38	129
MS.gene34370.t1	MTR_1g103320	56.311	103	44	1	53	154	11	113	1.46e-32	113

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34370.t1	AT2G32030	46.237	186	95	3	3	187	4	185	8.64e-56	175
MS.gene34370.t1	AT2G32020	51.497	167	76	3	22	187	18	180	1.39e-54	172
MS.gene34370.t1	AT3G22560	39.655	174	98	2	18	187	2	172	7.04e-36	124

Find 48 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTGGTTGATGCTGCATTTA+AGG	0.177178	1.2:-76764488	MS.gene34370:CDS
TTCTTTGAGCTCCATGTATT+GGG	0.213171	1.2:-76764542	MS.gene34370:CDS
CTCTTCCTTGGCATCTGATT+TGG	0.234671	1.2:+76764837	None:intergenic
ATTCTTTGAGCTCCATGTAT+TGG	0.293943	1.2:-76764543	MS.gene34370:CDS
TGCCTTCGCGATCGAGCAAT+TGG	0.297095	1.2:-76764625	MS.gene34370:CDS
GCCTCAAGCCTCTCCAAGTA+TGG	0.329009	1.2:+76764460	None:intergenic
GAGATTTGCTTGCTATGTTT+TGG	0.329625	1.2:+76764665	None:intergenic
GGGCAAAGGGATTGCCACTA+TGG	0.352044	1.2:-76764521	MS.gene34370:CDS
TCAAGATCAGAAAGACTAAT+TGG	0.352059	1.2:+76764778	None:intergenic
CCTTATACAAACAAAGAAAA+AGG	0.352282	1.2:-76764700	MS.gene34370:CDS
CTTGGCATCTGATTTGGAAT+AGG	0.366431	1.2:+76764843	None:intergenic
AATTGGGTGTACCGAGTTCC+AGG	0.375481	1.2:-76764608	MS.gene34370:CDS
CAGAAAATATTTGTTCATCA+AGG	0.394310	1.2:-76764365	MS.gene34370:CDS
ATTTAAGGAGTTTCCATACT+TGG	0.408342	1.2:-76764473	MS.gene34370:CDS
ACATAGCAAGCAAATCTCTA+TGG	0.412043	1.2:-76764660	MS.gene34370:CDS
TACCGAGTTCCAGGTGTATT+CGG	0.413001	1.2:-76764599	MS.gene34370:CDS
CAAAGGGTGTTGGAGAAGGC+TGG	0.447279	1.2:-76764409	MS.gene34370:CDS
TAATTGGTCGAAGAGAGATT+TGG	0.457252	1.2:+76764794	None:intergenic
CGGGACAAATCTGCTCAACT+TGG	0.459831	1.2:-76764568	MS.gene34370:CDS
GCCTTCGCGATCGAGCAATT+GGG	0.464374	1.2:-76764624	MS.gene34370:CDS
AGCTCCATGTATTGGGGCAA+AGG	0.494926	1.2:-76764535	MS.gene34370:CDS
AATCCCTTTGCCCCAATACA+TGG	0.496567	1.2:+76764531	None:intergenic
AGAAAATATTTGTTCATCAA+GGG	0.502192	1.2:-76764364	MS.gene34370:CDS
CACTTGTTTCACAACCATAG+TGG	0.504807	1.2:+76764507	None:intergenic
AACAACAGAATACTCTTCCT+TGG	0.516633	1.2:+76764825	None:intergenic
GCTCCATGTATTGGGGCAAA+GGG	0.517436	1.2:-76764534	MS.gene34370:CDS
ATCTTGATGATGTTATGTTG+TGG	0.524636	1.2:-76764762	MS.gene34370:CDS
AGGTTCCCAAGTGCAATACT+TGG	0.527166	1.2:+76764720	None:intergenic
CAAGTTGAGCAGATTTGTCC+CGG	0.527345	1.2:+76764569	None:intergenic
AGGTGTATTCGGATAGATGC+CGG	0.527922	1.2:-76764588	MS.gene34370:CDS
TGTGGCTTCACAAAGGGTGT+TGG	0.533258	1.2:-76764419	MS.gene34370:CDS
TTCACAAAGGGTGTTGGAGA+AGG	0.546969	1.2:-76764413	MS.gene34370:CDS
TCCATACTTGGAGAGGCTTG+AGG	0.557426	1.2:-76764461	MS.gene34370:CDS
GCTTGAGGCTCGAGTTGATG+TGG	0.569120	1.2:-76764446	MS.gene34370:CDS
GGCATCTATCCGAATACACC+TGG	0.571236	1.2:+76764590	None:intergenic
AGGAGTTTCCATACTTGGAG+AGG	0.574247	1.2:-76764468	MS.gene34370:CDS
GGTGTATTCGGATAGATGCC+GGG	0.580751	1.2:-76764587	MS.gene34370:CDS
CTATTCCAAATCAGATGCCA+AGG	0.586783	1.2:-76764842	MS.gene34370:CDS
TCTTTGAGCTCCATGTATTG+GGG	0.587210	1.2:-76764541	MS.gene34370:CDS
AAGTGGCCAAGTATTGCACT+TGG	0.595225	1.2:-76764726	MS.gene34370:CDS
GGAAAATGTGGCTTCACAAA+GGG	0.602523	1.2:-76764425	MS.gene34370:CDS
GTGGACTAGTGATGCAAAAG+TGG	0.603948	1.2:-76764743	MS.gene34370:CDS
ATCCGAATACACCTGGAACT+CGG	0.612102	1.2:+76764597	None:intergenic
ACCCAATTGCTCGATCGCGA+AGG	0.639639	1.2:+76764623	None:intergenic
TGGAAAATGTGGCTTCACAA+AGG	0.640244	1.2:-76764426	MS.gene34370:CDS
TCGAGTTGATGTGGAAAATG+TGG	0.669414	1.2:-76764437	MS.gene34370:CDS
AGTGGCCAAGTATTGCACTT+GGG	0.670287	1.2:-76764725	MS.gene34370:CDS
CACTATGGTTGTGAAACAAG+TGG	0.680187	1.2:-76764506	MS.gene34370:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGAAAATATTTGTTCATCAA+GGG	-	chr1.2:76764804-76764823	MS.gene34370:CDS	20.0%
!	CAGAAAATATTTGTTCATCA+AGG	-	chr1.2:76764803-76764822	MS.gene34370:CDS	25.0%
!	CCTTATACAAACAAAGAAAA+AGG	-	chr1.2:76764468-76764487	MS.gene34370:CDS	25.0%
!!!	CCTTTTTCTTTGTTTGTATA+AGG	+	chr1.2:76764471-76764490	None:intergenic	25.0%
	ATTTAAGGAGTTTCCATACT+TGG	-	chr1.2:76764695-76764714	MS.gene34370:CDS	30.0%
	TCAAGATCAGAAAGACTAAT+TGG	+	chr1.2:76764393-76764412	None:intergenic	30.0%
!!	ATCTTGATGATGTTATGTTG+TGG	-	chr1.2:76764406-76764425	MS.gene34370:CDS	30.0%
	AACAACAGAATACTCTTCCT+TGG	+	chr1.2:76764346-76764365	None:intergenic	35.0%
	ACATAGCAAGCAAATCTCTA+TGG	-	chr1.2:76764508-76764527	MS.gene34370:CDS	35.0%
	ATTCTTTGAGCTCCATGTAT+TGG	-	chr1.2:76764625-76764644	MS.gene34370:CDS	35.0%
	TAATTGGTCGAAGAGAGATT+TGG	+	chr1.2:76764377-76764396	None:intergenic	35.0%
	TTCTTTGAGCTCCATGTATT+GGG	-	chr1.2:76764626-76764645	MS.gene34370:CDS	35.0%
!!!	GAGATTTGCTTGCTATGTTT+TGG	+	chr1.2:76764506-76764525	None:intergenic	35.0%
	CACTATGGTTGTGAAACAAG+TGG	-	chr1.2:76764662-76764681	MS.gene34370:CDS	40.0%
	CACTTGTTTCACAACCATAG+TGG	+	chr1.2:76764664-76764683	None:intergenic	40.0%
	CTATTCCAAATCAGATGCCA+AGG	-	chr1.2:76764326-76764345	MS.gene34370:CDS	40.0%
	GGAAAATGTGGCTTCACAAA+GGG	-	chr1.2:76764743-76764762	MS.gene34370:CDS	40.0%
	TCTTTGAGCTCCATGTATTG+GGG	-	chr1.2:76764627-76764646	MS.gene34370:CDS	40.0%
	TGGAAAATGTGGCTTCACAA+AGG	-	chr1.2:76764742-76764761	MS.gene34370:CDS	40.0%
!	AGTGGTTGATGCTGCATTTA+AGG	-	chr1.2:76764680-76764699	MS.gene34370:CDS	40.0%
!!	CTTGGCATCTGATTTGGAAT+AGG	+	chr1.2:76764328-76764347	None:intergenic	40.0%
!!	TCGAGTTGATGTGGAAAATG+TGG	-	chr1.2:76764731-76764750	MS.gene34370:CDS	40.0%
	AATCCCTTTGCCCCAATACA+TGG	+	chr1.2:76764640-76764659	None:intergenic	45.0%
	AGGAGTTTCCATACTTGGAG+AGG	-	chr1.2:76764700-76764719	MS.gene34370:CDS	45.0%
	AGGTGTATTCGGATAGATGC+CGG	-	chr1.2:76764580-76764599	MS.gene34370:CDS	45.0%
	AGGTTCCCAAGTGCAATACT+TGG	+	chr1.2:76764451-76764470	None:intergenic	45.0%
	ATCCGAATACACCTGGAACT+CGG	+	chr1.2:76764574-76764593	None:intergenic	45.0%
	CTCTTCCTTGGCATCTGATT+TGG	+	chr1.2:76764334-76764353	None:intergenic	45.0%
	TACCGAGTTCCAGGTGTATT+CGG	-	chr1.2:76764569-76764588	MS.gene34370:CDS	45.0%
!	AAGTGGCCAAGTATTGCACT+TGG	-	chr1.2:76764442-76764461	MS.gene34370:CDS	45.0%
!	AGTGGCCAAGTATTGCACTT+GGG	-	chr1.2:76764443-76764462	MS.gene34370:CDS	45.0%
!	CAAGTTGAGCAGATTTGTCC+CGG	+	chr1.2:76764602-76764621	None:intergenic	45.0%
!!	GTGGACTAGTGATGCAAAAG+TGG	-	chr1.2:76764425-76764444	MS.gene34370:CDS	45.0%
!!	TTCACAAAGGGTGTTGGAGA+AGG	-	chr1.2:76764755-76764774	MS.gene34370:CDS	45.0%
!!!	AAGGCTGGTTTTGAAAGGGA+AGG	-	chr1.2:76764774-76764793	MS.gene34370:CDS	45.0%
!!!	GGAGAAGGCTGGTTTTGAAA+GGG	-	chr1.2:76764770-76764789	MS.gene34370:CDS	45.0%
!!!	TGGAGAAGGCTGGTTTTGAA+AGG	-	chr1.2:76764769-76764788	MS.gene34370:CDS	45.0%
	AATTGGGTGTACCGAGTTCC+AGG	-	chr1.2:76764560-76764579	MS.gene34370:CDS	50.0%
	AGCTCCATGTATTGGGGCAA+AGG	-	chr1.2:76764633-76764652	MS.gene34370:CDS	50.0%
	CGGGACAAATCTGCTCAACT+TGG	-	chr1.2:76764600-76764619	MS.gene34370:CDS	50.0%
	GCTCCATGTATTGGGGCAAA+GGG	-	chr1.2:76764634-76764653	MS.gene34370:CDS	50.0%
	GGCATCTATCCGAATACACC+TGG	+	chr1.2:76764581-76764600	None:intergenic	50.0%
	GGTGTATTCGGATAGATGCC+GGG	-	chr1.2:76764581-76764600	MS.gene34370:CDS	50.0%
	TCCATACTTGGAGAGGCTTG+AGG	-	chr1.2:76764707-76764726	MS.gene34370:CDS	50.0%
!	TGTGGCTTCACAAAGGGTGT+TGG	-	chr1.2:76764749-76764768	MS.gene34370:CDS	50.0%
	ACCCAATTGCTCGATCGCGA+AGG	+	chr1.2:76764548-76764567	None:intergenic	55.0%
	GCCTCAAGCCTCTCCAAGTA+TGG	+	chr1.2:76764711-76764730	None:intergenic	55.0%
	GCCTTCGCGATCGAGCAATT+GGG	-	chr1.2:76764544-76764563	MS.gene34370:CDS	55.0%
	GGGCAAAGGGATTGCCACTA+TGG	-	chr1.2:76764647-76764666	MS.gene34370:CDS	55.0%
	TGCCTTCGCGATCGAGCAAT+TGG	-	chr1.2:76764543-76764562	MS.gene34370:CDS	55.0%
!!	CAAAGGGTGTTGGAGAAGGC+TGG	-	chr1.2:76764759-76764778	MS.gene34370:CDS	55.0%
!!	GCTTGAGGCTCGAGTTGATG+TGG	-	chr1.2:76764722-76764741	MS.gene34370:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	76764309	76764881	76764309	ID=MS.gene34370
chr1.2	mRNA	76764309	76764881	76764309	ID=MS.gene34370.t1;Parent=MS.gene34370
chr1.2	exon	76764309	76764881	76764309	ID=MS.gene34370.t1.exon1;Parent=MS.gene34370.t1
chr1.2	CDS	76764309	76764881	76764309	ID=cds.MS.gene34370.t1;Parent=MS.gene34370.t1

Gene Sequence

>MS.gene34370

ATGGAAGAAACTACTACCTATTCCAAATCAGATGCCAAGGAAGAGTATTCTGTTGTTGACTTGAGCCAAATCTCTCTTCGACCAATTAGTCTTTCTGATCTTGATGATGTTATGTTGTGGACTAGTGATGCAAAAGTGGCCAAGTATTGCACTTGGGAACCTTATACAAACAAAGAAAAAGGTATTGACTTCATCCAAAACATAGCAAGCAAATCTCTATGGTTCAGAGCAATTTGCCTTCGCGATCGAGCAATTGGGTGTACCGAGTTCCAGGTGTATTCGGATAGATGCCGGGACAAATCTGCTCAACTTGGCTATTCTTTGAGCTCCATGTATTGGGGCAAAGGGATTGCCACTATGGTTGTGAAACAAGTGGTTGATGCTGCATTTAAGGAGTTTCCATACTTGGAGAGGCTTGAGGCTCGAGTTGATGTGGAAAATGTGGCTTCACAAAGGGTGTTGGAGAAGGCTGGTTTTGAAAGGGAAGGTATTCTCAGAAAATATTTGTTCATCAAGGGAAAAAGTATAGATATTGTTATGTTCAGTATTCTCTCAAGTGAAGTTAAACTTTGA

Protein sequence

>MS.gene34370.t1

MEETTTYSKSDAKEEYSVVDLSQISLRPISLSDLDDVMLWTSDAKVAKYCTWEPYTNKEKGIDFIQNIASKSLWFRAICLRDRAIGCTEFQVYSDRCRDKSAQLGYSLSSMYWGKGIATMVVKQVVDAAFKEFPYLERLEARVDVENVASQRVLEKAGFEREGILRKYLFIKGKSIDIVMFSILSSEVKL