AlfalfaGEDB | MS.gene21831

M. sativa cultivar XinJiangDaYe / MS.gene21831

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21831.t1	AES93696.1	78.8	179	33	4	1	175	1	178	2.30E-68	268.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21831.t1	P05332	30.4	158	97	6	15	165	2	153	5.9e-09	62.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21831.t1	G7K3P3	78.8	179	33	4	1	175	1	178	1.6e-68	268.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene21831.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene77154	MS.gene21831	PPI
MS.gene21831	MS.gene071357	PPI
MS.gene043813	MS.gene21831	PPI
MS.gene34371	MS.gene21831	PPI
MS.gene070945	MS.gene21831	PPI
MS.gene21831	MS.gene04530	PPI
MS.gene21833	MS.gene21831	PPI
MS.gene043815	MS.gene21831	PPI
MS.gene21831	MS.gene024614	PPI
MS.gene21828	MS.gene21831	PPI
MS.gene95802	MS.gene21831	PPI
MS.gene34370	MS.gene21831	PPI
MS.gene49472	MS.gene21831	PPI
MS.gene024859	MS.gene21831	PPI
MS.gene024855	MS.gene21831	PPI
MS.gene21831	MS.gene001034	PPI
MS.gene024612	MS.gene21831	PPI
MS.gene001038	MS.gene21831	PPI
MS.gene21831	MS.gene22888	PPI
MS.gene21830	MS.gene21831	PPI
MS.gene001034	MS.gene21831	PPI
MS.gene95801	MS.gene21831	PPI
MS.gene29937	MS.gene21831	PPI
MS.gene024858	MS.gene21831	PPI
MS.gene006418	MS.gene21831	PPI
MS.gene050493	MS.gene21831	PPI
MS.gene21827	MS.gene21831	PPI
MS.gene21831	MS.gene070944	PPI
MS.gene024854	MS.gene21831	PPI
MS.gene21831	MS.gene49470	PPI
MS.gene001037	MS.gene21831	PPI
MS.gene006417	MS.gene21831	PPI
MS.gene001033	MS.gene21831	PPI
MS.gene21831	MS.gene001032	PPI
MS.gene071361	MS.gene21831	PPI
MS.gene21831	MS.gene74082	PPI
MS.gene49468	MS.gene21831	PPI
MS.gene21831	MS.gene49471	PPI
MS.gene21831	MS.gene024857	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21831.t1	MTR_5g006460	78.771	179	33	4	1	175	1	178	3.03e-93	269
MS.gene21831.t1	MTR_5g006510	71.591	176	45	2	1	176	7	177	4.13e-85	249
MS.gene21831.t1	MTR_5g006450	67.978	178	53	3	1	176	1	176	3.35e-76	226
MS.gene21831.t1	MTR_5g006470	67.045	176	54	3	1	175	1	173	1.45e-75	224
MS.gene21831.t1	MTR_5g006520	65.341	176	59	2	1	175	1719	1893	6.89e-69	226
MS.gene21831.t1	MTR_1g103110	59.172	169	61	5	1	165	1	165	2.33e-58	181
MS.gene21831.t1	MTR_1g103150	55.866	179	71	5	1	175	1	175	1.89e-57	179
MS.gene21831.t1	MTR_7g024780	38.217	157	93	2	16	172	3	155	6.14e-30	108
MS.gene21831.t1	MTR_7g114410	36.943	157	96	1	17	173	4	157	2.70e-29	106
MS.gene21831.t1	MTR_7g114410	37.584	149	90	1	17	165	4	149	1.88e-28	104
MS.gene21831.t1	MTR_7g024760	40.278	144	79	4	20	162	7	144	3.72e-28	103
MS.gene21831.t1	MTR_7g024765	38.312	154	88	5	20	172	7	154	8.75e-28	102
MS.gene21831.t1	MTR_3g463610	36.242	149	95	0	17	165	3	151	2.42e-25	97.4
MS.gene21831.t1	MTR_1g103320	52.439	82	37	1	50	131	10	89	1.08e-19	80.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21831.t1	AT2G32020	48.077	156	70	6	20	172	18	165	2.16e-36	125
MS.gene21831.t1	AT2G32030	48.387	155	69	6	21	172	24	170	3.22e-35	122
MS.gene21831.t1	AT3G22560	33.987	153	94	3	16	165	2	150	1.50e-21	86.7

Find 38 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGAAGTATGTATTCTTTAA+AGG	0.161729	5.2:-1235077	MS.gene21831:CDS
AGAATCAAATGTGCAGAAAT+TGG	0.218349	5.2:-1235280	MS.gene21831:CDS
ATGAAAAGGTAGCCAAGTTC+TGG	0.273400	5.2:-1235447	MS.gene21831:CDS
CTCTTCATTGGCACATGGTT+TGG	0.276019	5.2:+1235549	None:intergenic
GCTTCAAGCCTTTCCAAGTA+TGG	0.276638	5.2:+1235172	None:intergenic
ATGTTCTAGGCTCTAAATAT+TGG	0.292142	5.2:-1235255	MS.gene21831:CDS
TGTTCTAGGCTCTAAATATT+GGG	0.316974	5.2:-1235254	MS.gene21831:CDS
TTGTTGATGTGGATAATTTA+TGG	0.328127	5.2:-1235147	MS.gene21831:CDS
TGCCATAATGATCATGCTAT+TGG	0.333891	5.2:-1235343	MS.gene21831:CDS
ACCCAATAGCATGATCATTA+TGG	0.341789	5.2:+1235341	None:intergenic
GCAGAAATTGGGTATGTTCT+AGG	0.344662	5.2:-1235268	MS.gene21831:CDS
CCTTCGTCTTTGCTAGTGTA+AGG	0.376096	5.2:+1235418	None:intergenic
CAAATCAATGACCTCTTCAT+TGG	0.385362	5.2:+1235537	None:intergenic
GGCTCTAAATATTGGGGTAA+AGG	0.406739	5.2:-1235247	MS.gene21831:CDS
ATTCAGTGAATTTCCATACT+TGG	0.413337	5.2:-1235185	MS.gene21831:CDS
CACGTTTGCTGTGAAACAAA+TGG	0.422199	5.2:-1235218	MS.gene21831:CDS
GCTCTAAATATTGGGGTAAA+GGG	0.428389	5.2:-1235246	MS.gene21831:CDS
TTCTGACCTTGATGATGTAA+TGG	0.440976	5.2:-1235485	MS.gene21831:CDS
CAAAGAGTAGTTGAGAAAGT+TGG	0.450786	5.2:-1235121	MS.gene21831:CDS
GAAGATGTCGGAGTGTGATT+TGG	0.453912	5.2:+1235512	None:intergenic
GAATCAAATGTGCAGAAATT+GGG	0.456981	5.2:-1235279	MS.gene21831:CDS
TGCTGTGAAACAAATGGTTA+AGG	0.473285	5.2:-1235212	MS.gene21831:CDS
GCCATAATGATCATGCTATT+GGG	0.478546	5.2:-1235342	MS.gene21831:CDS
TGAAAAGGTAGCCAAGTTCT+GGG	0.482152	5.2:-1235446	MS.gene21831:CDS
GTGAATTTCCATACTTGGAA+AGG	0.490341	5.2:-1235180	MS.gene21831:CDS
GCTTGAAGCTCTTGTTGATG+TGG	0.580383	5.2:-1235158	MS.gene21831:CDS
CAAACCATGTGCCAATGAAG+AGG	0.589377	5.2:-1235548	MS.gene21831:CDS
GTGGCAGCAGACTGATGAAA+AGG	0.594266	5.2:-1235461	MS.gene21831:CDS
GCCACACCATTACATCATCA+AGG	0.594693	5.2:+1235479	None:intergenic
ATGACCTCTTCATTGGCACA+TGG	0.616227	5.2:+1235544	None:intergenic
AAAAGGTAGCCAAGTTCTGG+GGG	0.620200	5.2:-1235444	MS.gene21831:CDS
GAAAAGGTAGCCAAGTTCTG+GGG	0.624494	5.2:-1235445	MS.gene21831:CDS
GTTCTAGGCTCTAAATATTG+GGG	0.628624	5.2:-1235253	MS.gene21831:CDS
GGTCAGAAAGATGAAGATGT+CGG	0.630507	5.2:+1235500	None:intergenic
CATTTGTTTCACAGCAAACG+TGG	0.659491	5.2:+1235219	None:intergenic
ACCTTGATGATGTAATGGTG+TGG	0.697032	5.2:-1235480	MS.gene21831:CDS
CCTTACACTAGCAAAGACGA+AGG	0.715657	5.2:-1235418	MS.gene21831:CDS
AAAGGTAGCCAAGTTCTGGG+GGG	0.779565	5.2:-1235443	MS.gene21831:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTGTTGATGTGGATAATTTA+TGG	-	chr5.2:1235484-1235503	MS.gene21831:CDS	25.0%
!!	AGGAAGTATGTATTCTTTAA+AGG	-	chr5.2:1235554-1235573	MS.gene21831:CDS	25.0%
	AGAATCAAATGTGCAGAAAT+TGG	-	chr5.2:1235351-1235370	MS.gene21831:CDS	30.0%
	ATTCAGTGAATTTCCATACT+TGG	-	chr5.2:1235446-1235465	MS.gene21831:CDS	30.0%
	GAATCAAATGTGCAGAAATT+GGG	-	chr5.2:1235352-1235371	MS.gene21831:CDS	30.0%
	TTTCAAAAAGAGGAGTTCTT+AGG	-	chr5.2:1235534-1235553	MS.gene21831:CDS	30.0%
!!	ATGTTCTAGGCTCTAAATAT+TGG	-	chr5.2:1235376-1235395	MS.gene21831:CDS	30.0%
!!	TGTTCTAGGCTCTAAATATT+GGG	-	chr5.2:1235377-1235396	MS.gene21831:CDS	30.0%
!!!	GAAAGTTGGTTTTCAAAAAG+AGG	-	chr5.2:1235524-1235543	MS.gene21831:CDS	30.0%
	ACCCAATAGCATGATCATTA+TGG	+	chr5.2:1235293-1235312	None:intergenic	35.0%
	CAAAGAGTAGTTGAGAAAGT+TGG	-	chr5.2:1235510-1235529	MS.gene21831:CDS	35.0%
	CAAATCAATGACCTCTTCAT+TGG	+	chr5.2:1235097-1235116	None:intergenic	35.0%
	GCCATAATGATCATGCTATT+GGG	-	chr5.2:1235289-1235308	MS.gene21831:CDS	35.0%
	GTGAATTTCCATACTTGGAA+AGG	-	chr5.2:1235451-1235470	MS.gene21831:CDS	35.0%
	TGCCATAATGATCATGCTAT+TGG	-	chr5.2:1235288-1235307	MS.gene21831:CDS	35.0%
	TGCTGTGAAACAAATGGTTA+AGG	-	chr5.2:1235419-1235438	MS.gene21831:CDS	35.0%
!	GCTCTAAATATTGGGGTAAA+GGG	-	chr5.2:1235385-1235404	MS.gene21831:CDS	35.0%
!!	GTTCTAGGCTCTAAATATTG+GGG	-	chr5.2:1235378-1235397	MS.gene21831:CDS	35.0%
!!	TTCTGACCTTGATGATGTAA+TGG	-	chr5.2:1235146-1235165	MS.gene21831:CDS	35.0%
	ATGAAAAGGTAGCCAAGTTC+TGG	-	chr5.2:1235184-1235203	MS.gene21831:CDS	40.0%
	CATTTGTTTCACAGCAAACG+TGG	+	chr5.2:1235415-1235434	None:intergenic	40.0%
	GCAGAAATTGGGTATGTTCT+AGG	-	chr5.2:1235363-1235382	MS.gene21831:CDS	40.0%
	GGTCAGAAAGATGAAGATGT+CGG	+	chr5.2:1235134-1235153	None:intergenic	40.0%
	TGAAAAGGTAGCCAAGTTCT+GGG	-	chr5.2:1235185-1235204	MS.gene21831:CDS	40.0%
!!	ACCTTGATGATGTAATGGTG+TGG	-	chr5.2:1235151-1235170	MS.gene21831:CDS	40.0%
!!	CACGTTTGCTGTGAAACAAA+TGG	-	chr5.2:1235413-1235432	MS.gene21831:CDS	40.0%
!!	GGCTCTAAATATTGGGGTAA+AGG	-	chr5.2:1235384-1235403	MS.gene21831:CDS	40.0%
	ATGACCTCTTCATTGGCACA+TGG	+	chr5.2:1235090-1235109	None:intergenic	45.0%
	CAAACCATGTGCCAATGAAG+AGG	-	chr5.2:1235083-1235102	MS.gene21831:CDS	45.0%
	CCTTACACTAGCAAAGACGA+AGG	-	chr5.2:1235213-1235232	MS.gene21831:CDS	45.0%
	CCTTCGTCTTTGCTAGTGTA+AGG	+	chr5.2:1235216-1235235	None:intergenic	45.0%
	CTCTTCATTGGCACATGGTT+TGG	+	chr5.2:1235085-1235104	None:intergenic	45.0%
	GAAAAGGTAGCCAAGTTCTG+GGG	-	chr5.2:1235186-1235205	MS.gene21831:CDS	45.0%
	GAAGATGTCGGAGTGTGATT+TGG	+	chr5.2:1235122-1235141	None:intergenic	45.0%
	GCCACACCATTACATCATCA+AGG	+	chr5.2:1235155-1235174	None:intergenic	45.0%
	GCTTCAAGCCTTTCCAAGTA+TGG	+	chr5.2:1235462-1235481	None:intergenic	45.0%
	GCTTGAAGCTCTTGTTGATG+TGG	-	chr5.2:1235473-1235492	MS.gene21831:CDS	45.0%
!	AAAAGGTAGCCAAGTTCTGG+GGG	-	chr5.2:1235187-1235206	MS.gene21831:CDS	45.0%
	GTGGCAGCAGACTGATGAAA+AGG	-	chr5.2:1235170-1235189	MS.gene21831:CDS	50.0%
!!	AAAGGTAGCCAAGTTCTGGG+GGG	-	chr5.2:1235188-1235207	MS.gene21831:CDS	50.0%
	GTAAGGTTCCCCCCAGAACT+TGG	+	chr5.2:1235199-1235218	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	1235060	1235593	1235060	ID=MS.gene21831
chr5.2	mRNA	1235060	1235593	1235060	ID=MS.gene21831.t1;Parent=MS.gene21831
chr5.2	exon	1235060	1235593	1235060	ID=MS.gene21831.t1.exon1;Parent=MS.gene21831.t1
chr5.2	CDS	1235060	1235593	1235060	ID=cds.MS.gene21831.t1;Parent=MS.gene21831.t1

Gene Sequence

>MS.gene21831

ATGGAAGAGACTTCTATTTTCTCCAAACCATGTGCCAATGAAGAGGTCATTGATTTGAACCAAATCACACTCCGACATCTTCATCTTTCTGACCTTGATGATGTAATGGTGTGGCAGCAGACTGATGAAAAGGTAGCCAAGTTCTGGGGGGAACCTTACACTAGCAAAGACGAAGGCATCAACTTCATTGAAAATATAGAAAGTGATTATCTACGTTGCGAAGCAATATGCCATAATGATCATGCTATTGGGTGTATTAAGTTGTTCTCGAGTTCACGTCATGATCTAAGCAGAATCAAATGTGCAGAAATTGGGTATGTTCTAGGCTCTAAATATTGGGGTAAAGGGATTACCACGTTTGCTGTGAAACAAATGGTTAAGGTTGCATTCAGTGAATTTCCATACTTGGAAAGGCTTGAAGCTCTTGTTGATGTGGATAATTTATGGTCGCAAAGAGTAGTTGAGAAAGTTGGTTTTCAAAAAGAGGAGTTCTTAGGAAGTATGTATTCTTTAAAGGAAAAAGCAGAGATATGA

Protein sequence

>MS.gene21831.t1

MEETSIFSKPCANEEVIDLNQITLRHLHLSDLDDVMVWQQTDEKVAKFWGEPYTSKDEGINFIENIESDYLRCEAICHNDHAIGCIKLFSSSRHDLSRIKCAEIGYVLGSKYWGKGITTFAVKQMVKVAFSEFPYLERLEALVDVDNLWSQRVVEKVGFQKEEFLGSMYSLKEKAEI