AlfalfaGEDB | MS.gene001037

M. sativa cultivar XinJiangDaYe / MS.gene001037

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001037.t1	XP_003610742.1	87	193	23	1	1	193	1	191	9.60E-87	329.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001037.t1	P05332	31.3	179	111	4	19	190	2	175	2.9e-15	83.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001037.t1	G7K3P7	87.0	193	23	1	1	193	1	191	6.9e-87	329.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene001037.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene29937	MS.gene001037	PPI
MS.gene024858	MS.gene001037	PPI
MS.gene006418	MS.gene001037	PPI
MS.gene001037	MS.gene49470	PPI
MS.gene001037	MS.gene001032	PPI
MS.gene024854	MS.gene001037	PPI
MS.gene001037	MS.gene74082	PPI
MS.gene001037	MS.gene49471	PPI
MS.gene001033	MS.gene001037	PPI
MS.gene001037	MS.gene071357	PPI
MS.gene001037	MS.gene050493	PPI
MS.gene071361	MS.gene001037	PPI
MS.gene001037	MS.gene21828	PPI
MS.gene49468	MS.gene001037	PPI
MS.gene001037	MS.gene22888	PPI
MS.gene34370	MS.gene001037	PPI
MS.gene001037	MS.gene04530	PPI
MS.gene001037	MS.gene024614	PPI
MS.gene77154	MS.gene001037	PPI
MS.gene34371	MS.gene001037	PPI
MS.gene001037	MS.gene34371	PPI
MS.gene001037	MS.gene001034	PPI
MS.gene070945	MS.gene001037	PPI
MS.gene21833	MS.gene001037	PPI
MS.gene043815	MS.gene001037	PPI
MS.gene21828	MS.gene001037	PPI
MS.gene95802	MS.gene001037	PPI
MS.gene001037	MS.gene043813	PPI
MS.gene49472	MS.gene001037	PPI
MS.gene001037	MS.gene21831	PPI
MS.gene024859	MS.gene001037	PPI
MS.gene024855	MS.gene001037	PPI
MS.gene024612	MS.gene001037	PPI
MS.gene001037	MS.gene006417	PPI
MS.gene001038	MS.gene001037	PPI
MS.gene21830	MS.gene001037	PPI
MS.gene001037	MS.gene21827	PPI
MS.gene001037	MS.gene024857	PPI
MS.gene001037	MS.gene070944	PPI
MS.gene001037	MS.gene95801	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001037.t1	MTR_5g006510	87.047	193	23	1	1	193	1	191	4.28e-121	341
MS.gene001037.t1	MTR_5g006460	80.311	193	30	3	9	193	1	193	2.10e-107	306
MS.gene001037.t1	MTR_5g006470	69.634	191	49	4	9	193	1	188	1.90e-88	258
MS.gene001037.t1	MTR_5g006450	70.157	191	50	3	9	193	1	190	6.36e-88	257
MS.gene001037.t1	MTR_5g006520	66.146	192	58	3	8	193	1718	1908	3.01e-76	249
MS.gene001037.t1	MTR_1g103110	60.989	182	64	4	16	193	12	190	7.75e-73	219
MS.gene001037.t1	MTR_1g103150	59.091	176	67	3	20	193	18	190	4.33e-70	212
MS.gene001037.t1	MTR_7g114410	43.605	172	91	3	21	190	4	171	6.89e-42	140
MS.gene001037.t1	MTR_7g024760	46.429	168	84	4	24	190	7	169	3.75e-41	138
MS.gene001037.t1	MTR_7g024780	44.767	172	90	3	20	190	3	170	5.25e-41	137
MS.gene001037.t1	MTR_3g463610	41.520	171	99	1	21	190	3	173	5.76e-40	136
MS.gene001037.t1	MTR_7g114410	43.671	158	83	3	21	176	4	157	3.30e-38	130
MS.gene001037.t1	MTR_7g024765	44.379	169	86	4	24	190	7	169	9.79e-38	129
MS.gene001037.t1	MTR_1g103320	50.000	82	39	1	53	134	10	89	1.63e-17	75.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001037.t1	AT2G32030	48.187	193	87	6	1	190	3	185	3.93e-53	169
MS.gene001037.t1	AT2G32020	51.176	170	73	5	24	190	18	180	5.79e-51	163
MS.gene001037.t1	AT3G22560	38.286	175	100	4	20	190	2	172	1.02e-32	116

Find 36 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTTGATGTGGATAATTTA+AGG	0.203228	5.4:-2233385	MS.gene001037:CDS
CAAATCAATGATCTCTTCTT+TGG	0.204931	5.4:+2233772	None:intergenic
GTTGATGCCTTGGTCTTTGC+TGG	0.262298	5.4:+2233649	None:intergenic
GCTTGAAGCCTTTCCAAGTA+TGG	0.276638	5.4:+2233410	None:intergenic
TGCCATAACGATCATGCTAT+TGG	0.288303	5.4:-2233581	MS.gene001037:CDS
GCAGAACTTGGGTATGTTCT+AGG	0.307665	5.4:-2233506	MS.gene001037:CDS
ATGTTCTAGGCTCTAAATAC+TGG	0.313478	5.4:-2233493	MS.gene001037:CDS
CCTTGGTCTTTGCTGGTGTA+AGG	0.327596	5.4:+2233656	None:intergenic
GAAAAGATCGGAGTGTGATT+TGG	0.386444	5.4:+2233747	None:intergenic
ACCTCGATGATCTCATGGTT+TGG	0.401354	5.4:-2233715	MS.gene001037:CDS
GGCTCTAAATACTGGGGTAA+AGG	0.409375	5.4:-2233485	MS.gene001037:CDS
ATTCAGTGAATTTCCATACT+TGG	0.413337	5.4:-2233423	MS.gene001037:CDS
GCCATAACGATCATGCTATT+GGG	0.423146	5.4:-2233580	MS.gene001037:CDS
TTCTGACCTCGATGATCTCA+TGG	0.433346	5.4:-2233720	MS.gene001037:CDS
GCTCTAAATACTGGGGTAAA+GGG	0.442106	5.4:-2233484	MS.gene001037:CDS
TGAGTTCACTTGATGATAAG+AGG	0.442621	5.4:-2233541	MS.gene001037:CDS
CAAAGAGTAGTTGAGAAAGT+TGG	0.450786	5.4:-2233359	MS.gene001037:CDS
AGGATCGCATGTGCAGAACT+TGG	0.454084	5.4:-2233518	MS.gene001037:CDS
ACCCAATAGCATGATCGTTA+TGG	0.457877	5.4:+2233579	None:intergenic
CTACTGATCTTAAACTAATG+AGG	0.474389	5.4:-2233256	MS.gene001037:CDS
GTAAGGTTCACCCCAGAACT+TGG	0.480302	5.4:+2233673	None:intergenic
TAAACTAATGAGGATGTTCA+AGG	0.483858	5.4:-2233246	MS.gene001037:CDS
GTGAATTTCCATACTTGGAA+AGG	0.490341	5.4:-2233418	MS.gene001037:CDS
TGCTGTGAAACAAATGGTTA+AGG	0.500098	5.4:-2233450	MS.gene001037:CDS
TGTTCTAGGCTCTAAATACT+GGG	0.526941	5.4:-2233492	MS.gene001037:CDS
CTTATCATCAAGTGAACTCA+AGG	0.556721	5.4:+2233544	None:intergenic
GCTTCAAGCTCTTGTTGATG+TGG	0.560091	5.4:-2233396	MS.gene001037:CDS
GGATCGCATGTGCAGAACTT+GGG	0.585194	5.4:-2233517	MS.gene001037:CDS
GGTCAGAAAGATGAAAAGAT+CGG	0.592412	5.4:+2233735	None:intergenic
CATTTGTTTCACAGCACATG+TGG	0.625218	5.4:+2233457	None:intergenic
TAATGAGGATGTTCAAGGTG+AGG	0.626914	5.4:-2233241	MS.gene001037:CDS
CACATGTGCTGTGAAACAAA+TGG	0.647600	5.4:-2233456	MS.gene001037:CDS
GTTCACTTGATGATAAGAGG+AGG	0.664638	5.4:-2233538	MS.gene001037:CDS
CCTTACACCAGCAAAGACCA+AGG	0.673207	5.4:-2233656	MS.gene001037:CDS
GTTCTAGGCTCTAAATACTG+GGG	0.712321	5.4:-2233491	MS.gene001037:CDS
GCCAAACCATGAGATCATCG+AGG	0.781144	5.4:+2233714	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CATCAACTTCATTAAAAATA+TGG	-	chr5.4:2233402-2233421	MS.gene001037:CDS	20.0%
!	TTGTTGATGTGGATAATTTA+AGG	-	chr5.4:2233650-2233669	MS.gene001037:CDS	25.0%
!!!	AAAGTTGGTTTTCAAAAAGA+GGG	-	chr5.4:2233691-2233710	MS.gene001037:CDS	25.0%
	ATTCAGTGAATTTCCATACT+TGG	-	chr5.4:2233612-2233631	MS.gene001037:CDS	30.0%
	CAAATCAATGATCTCTTCTT+TGG	+	chr5.4:2233266-2233285	None:intergenic	30.0%
	CTACTGATCTTAAACTAATG+AGG	-	chr5.4:2233779-2233798	MS.gene001037:CDS	30.0%
	TAAACTAATGAGGATGTTCA+AGG	-	chr5.4:2233789-2233808	MS.gene001037:CDS	30.0%
	TCATTAAAAATATGGCAAGC+AGG	-	chr5.4:2233410-2233429	MS.gene001037:CDS	30.0%
!!!	GAAAGTTGGTTTTCAAAAAG+AGG	-	chr5.4:2233690-2233709	MS.gene001037:CDS	30.0%
!!!	TTTTAATGAAGTTGATGCCT+TGG	+	chr5.4:2233399-2233418	None:intergenic	30.0%
	CAAAGAGTAGTTGAGAAAGT+TGG	-	chr5.4:2233676-2233695	MS.gene001037:CDS	35.0%
	CTTATCATCAAGTGAACTCA+AGG	+	chr5.4:2233494-2233513	None:intergenic	35.0%
	GGTCAGAAAGATGAAAAGAT+CGG	+	chr5.4:2233303-2233322	None:intergenic	35.0%
	GTGAATTTCCATACTTGGAA+AGG	-	chr5.4:2233617-2233636	MS.gene001037:CDS	35.0%
	TGCTGTGAAACAAATGGTTA+AGG	-	chr5.4:2233585-2233604	MS.gene001037:CDS	35.0%
	TTCAAAAAGAGGGAGTTCTT+AGG	-	chr5.4:2233701-2233720	MS.gene001037:CDS	35.0%
	TTGGCATACTGATGAAAAAG+TGG	-	chr5.4:2233339-2233358	MS.gene001037:CDS	35.0%
!	TGAGTTCACTTGATGATAAG+AGG	-	chr5.4:2233494-2233513	MS.gene001037:CDS	35.0%
!!	ATGTTCTAGGCTCTAAATAC+TGG	-	chr5.4:2233542-2233561	MS.gene001037:CDS	35.0%
!!	TGTTCTAGGCTCTAAATACT+GGG	-	chr5.4:2233543-2233562	MS.gene001037:CDS	35.0%
	ACCCAATAGCATGATCGTTA+TGG	+	chr5.4:2233459-2233478	None:intergenic	40.0%
	ATGAAAAAGTGGCCAAGTTC+TGG	-	chr5.4:2233350-2233369	MS.gene001037:CDS	40.0%
	CACATGTGCTGTGAAACAAA+TGG	-	chr5.4:2233579-2233598	MS.gene001037:CDS	40.0%
	CATTTGTTTCACAGCACATG+TGG	+	chr5.4:2233581-2233600	None:intergenic	40.0%
	GAAAAGATCGGAGTGTGATT+TGG	+	chr5.4:2233291-2233310	None:intergenic	40.0%
	GCCATAACGATCATGCTATT+GGG	-	chr5.4:2233455-2233474	MS.gene001037:CDS	40.0%
	TAATGAGGATGTTCAAGGTG+AGG	-	chr5.4:2233794-2233813	MS.gene001037:CDS	40.0%
	TGAAAAAGTGGCCAAGTTCT+GGG	-	chr5.4:2233351-2233370	MS.gene001037:CDS	40.0%
	TGCCATAACGATCATGCTAT+TGG	-	chr5.4:2233454-2233473	MS.gene001037:CDS	40.0%
!	GCTCTAAATACTGGGGTAAA+GGG	-	chr5.4:2233551-2233570	MS.gene001037:CDS	40.0%
!	GTTCACTTGATGATAAGAGG+AGG	-	chr5.4:2233497-2233516	MS.gene001037:CDS	40.0%
!!	GTTCTAGGCTCTAAATACTG+GGG	-	chr5.4:2233544-2233563	MS.gene001037:CDS	40.0%
	ACCTCGATGATCTCATGGTT+TGG	-	chr5.4:2233320-2233339	MS.gene001037:CDS	45.0%
	GAAAAAGTGGCCAAGTTCTG+GGG	-	chr5.4:2233352-2233371	MS.gene001037:CDS	45.0%
	GCAGAACTTGGGTATGTTCT+AGG	-	chr5.4:2233529-2233548	MS.gene001037:CDS	45.0%
	GCTTCAAGCTCTTGTTGATG+TGG	-	chr5.4:2233639-2233658	MS.gene001037:CDS	45.0%
	GCTTGAAGCCTTTCCAAGTA+TGG	+	chr5.4:2233628-2233647	None:intergenic	45.0%
	TTCTGACCTCGATGATCTCA+TGG	-	chr5.4:2233315-2233334	MS.gene001037:CDS	45.0%
!!	GGCTCTAAATACTGGGGTAA+AGG	-	chr5.4:2233550-2233569	MS.gene001037:CDS	45.0%
	AGGATCGCATGTGCAGAACT+TGG	-	chr5.4:2233517-2233536	MS.gene001037:CDS	50.0%
	CCTTACACCAGCAAAGACCA+AGG	-	chr5.4:2233379-2233398	MS.gene001037:CDS	50.0%
	GCCAAACCATGAGATCATCG+AGG	+	chr5.4:2233324-2233343	None:intergenic	50.0%
	GGATCGCATGTGCAGAACTT+GGG	-	chr5.4:2233518-2233537	MS.gene001037:CDS	50.0%
	GTAAGGTTCACCCCAGAACT+TGG	+	chr5.4:2233365-2233384	None:intergenic	50.0%
!	CCTTGGTCTTTGCTGGTGTA+AGG	+	chr5.4:2233382-2233401	None:intergenic	50.0%
!	GTTGATGCCTTGGTCTTTGC+TGG	+	chr5.4:2233389-2233408	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	2233217	2233840	2233217	ID=MS.gene001037
chr5.4	mRNA	2233217	2233840	2233217	ID=MS.gene001037.t1;Parent=MS.gene001037
chr5.4	exon	2233217	2233840	2233217	ID=MS.gene001037.t1.exon1;Parent=MS.gene001037.t1
chr5.4	CDS	2233217	2233840	2233217	ID=cds.MS.gene001037.t1;Parent=MS.gene001037.t1

Gene Sequence

>MS.gene001037

ATGGAGCAAAACACAAGTATTATGATAGAGCAACTTTCTATTTCTTCCAAAGAAGAGATCATTGATTTGAACCAAATCACACTCCGATCTTTTCATCTTTCTGACCTCGATGATCTCATGGTTTGGCATACTGATGAAAAAGTGGCCAAGTTCTGGGGTGAACCTTACACCAGCAAAGACCAAGGCATCAACTTCATTAAAAATATGGCAAGCAGGTATCTAAGTTGCAAAGCAATATGCCATAACGATCATGCTATTGGGTGTATTAAGTTGTCCTTGAGTTCACTTGATGATAAGAGGAGGATCGCATGTGCAGAACTTGGGTATGTTCTAGGCTCTAAATACTGGGGTAAAGGGATTGCCACATGTGCTGTGAAACAAATGGTTAAGGTTGCATTCAGTGAATTTCCATACTTGGAAAGGCTTCAAGCTCTTGTTGATGTGGATAATTTAAGGTCGCAAAGAGTAGTTGAGAAAGTTGGTTTTCAAAAAGAGGGAGTTCTTAGGAAGTATGTATTTTTTAAAGAAAAAAGCAGAGATATGATCATGTTCAGTCTTCTTTCTACTGATCTTAAACTAATGAGGATGTTCAAGGTGAGGAACTTATATATTGCTGAAATGTAA

Protein sequence

>MS.gene001037.t1

MEQNTSIMIEQLSISSKEEIIDLNQITLRSFHLSDLDDLMVWHTDEKVAKFWGEPYTSKDQGINFIKNMASRYLSCKAICHNDHAIGCIKLSLSSLDDKRRIACAELGYVLGSKYWGKGIATCAVKQMVKVAFSEFPYLERLQALVDVDNLRSQRVVEKVGFQKEGVLRKYVFFKEKSRDMIMFSLLSTDLKLMRMFKVRNLYIAEM