AlfalfaGEDB | MS.gene071357

M. sativa cultivar XinJiangDaYe / MS.gene071357

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071357.t1	XP_003610737.1	85.1	174	25	1	1	173	1	174	5.40E-75	290.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071357.t1	G7K3P2	85.1	174	25	1	1	173	1	174	3.9e-75	290.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene071357.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene001034	MS.gene071357	PPI
MS.gene21831	MS.gene071357	PPI
MS.gene95801	MS.gene071357	PPI
MS.gene024614	MS.gene071357	PPI
MS.gene29937	MS.gene071357	PPI
MS.gene024858	MS.gene071357	PPI
MS.gene006418	MS.gene071357	PPI
MS.gene070944	MS.gene071357	PPI
MS.gene050493	MS.gene071357	PPI
MS.gene21827	MS.gene071357	PPI
MS.gene74082	MS.gene071357	PPI
MS.gene024854	MS.gene071357	PPI
MS.gene071357	MS.gene024857	PPI
MS.gene001037	MS.gene071357	PPI
MS.gene006417	MS.gene071357	PPI
MS.gene49471	MS.gene071357	PPI
MS.gene001033	MS.gene071357	PPI
MS.gene071361	MS.gene071357	PPI
MS.gene49468	MS.gene071357	PPI
MS.gene04530	MS.gene071357	PPI
MS.gene22888	MS.gene071357	PPI
MS.gene001032	MS.gene071357	PPI
MS.gene043813	MS.gene071357	PPI
MS.gene024857	MS.gene071357	PPI
MS.gene070945	MS.gene071357	PPI
MS.gene21833	MS.gene071357	PPI
MS.gene043815	MS.gene071357	PPI
MS.gene21828	MS.gene071357	PPI
MS.gene95802	MS.gene071357	PPI
MS.gene34370	MS.gene071357	PPI
MS.gene49472	MS.gene071357	PPI
MS.gene024859	MS.gene071357	PPI
MS.gene024855	MS.gene071357	PPI
MS.gene77154	MS.gene071357	PPI
MS.gene024612	MS.gene071357	PPI
MS.gene34371	MS.gene071357	PPI
MS.gene001038	MS.gene071357	PPI
MS.gene49470	MS.gene071357	PPI
MS.gene21830	MS.gene071357	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071357.t1	MTR_5g006450	85.057	174	25	1	1	173	1	174	4.34e-103	294
MS.gene071357.t1	MTR_5g006470	69.540	174	50	2	1	173	1	172	4.93e-76	225
MS.gene071357.t1	MTR_5g006520	72.561	164	44	1	1	163	1719	1882	1.73e-74	243
MS.gene071357.t1	MTR_5g006510	68.293	164	44	4	1	162	7	164	8.32e-68	205
MS.gene071357.t1	MTR_5g006460	68.452	168	45	6	1	162	1	166	6.30e-66	200
MS.gene071357.t1	MTR_1g103110	59.394	165	62	3	1	162	1	163	1.33e-60	186
MS.gene071357.t1	MTR_1g103150	56.970	165	66	3	1	162	1	163	1.71e-57	179
MS.gene071357.t1	MTR_7g114410	35.294	136	85	1	16	151	3	135	3.59e-27	100
MS.gene071357.t1	MTR_7g114410	35.294	136	85	1	16	151	3	135	7.07e-27	100
MS.gene071357.t1	MTR_3g463610	33.813	139	92	0	17	155	3	141	1.62e-26	100
MS.gene071357.t1	MTR_1g103320	44.538	119	49	3	53	170	13	115	3.26e-23	89.0
MS.gene071357.t1	MTR_7g024765	32.026	153	98	3	20	171	7	154	5.32e-22	87.8
MS.gene071357.t1	MTR_7g024760	36.765	136	81	3	20	155	7	137	1.11e-21	87.0
MS.gene071357.t1	MTR_7g024780	32.026	153	99	3	17	168	4	152	4.47e-20	82.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071357.t1	AT2G32030	46.104	154	68	4	3	155	14	153	9.03e-40	133
MS.gene071357.t1	AT2G32020	47.794	136	64	2	21	155	19	148	3.77e-36	124
MS.gene071357.t1	AT3G22560	33.557	149	93	3	21	166	7	152	1.68e-20	84.0

Find 43 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCAAGGTCAGAAAGATTA+AGG	0.227074	2.1:+34189289	None:intergenic
CTCTTCTTTGTCACGTGGTT+TGG	0.270188	2.1:+34189344	None:intergenic
ATGTTCTAGGCTCTAAATAT+TGG	0.292142	2.1:-34189050	MS.gene071357:CDS
GCAGAACTTGGCTATGTTCT+AGG	0.302641	2.1:-34189063	MS.gene071357:CDS
TAAGGGGTCGGAGTGTGATT+TGG	0.307480	2.1:+34189307	None:intergenic
TGTTCTAGGCTCTAAATATT+GGG	0.316974	2.1:-34189049	MS.gene071357:CDS
AAGGGGTCGGAGTGTGATTT+GGG	0.320072	2.1:+34189308	None:intergenic
ATATAGCTTCAAAGTTTCTA+TGG	0.332117	2.1:-34189173	MS.gene071357:CDS
TCCATCAATGATCGTGCAAT+TGG	0.335248	2.1:-34189138	MS.gene071357:CDS
CCCAATTGCACGATCATTGA+TGG	0.338330	2.1:+34189137	None:intergenic
AATGTTGGGTCTCAGAGTTT+TGG	0.354229	2.1:-34188928	MS.gene071357:CDS
GCTCTAAATATTGGGGTAAA+GGG	0.356917	2.1:-34189041	MS.gene071357:CDS
GGCTCTAAATATTGGGGTAA+AGG	0.422886	2.1:-34189042	MS.gene071357:CDS
AGTTGCCAAGTTCTGCTCTT+GGG	0.428431	2.1:-34189238	MS.gene071357:CDS
GTTGATGCCATCATCCTTGC+TGG	0.429573	2.1:+34189206	None:intergenic
CACTTGTTTCACAACACACT+TGG	0.439429	2.1:+34189014	None:intergenic
ATTCTGTGAGTTGTCTTACT+TGG	0.446468	2.1:-34188980	MS.gene071357:CDS
AAGTTGCCAAGTTCTGCTCT+TGG	0.477212	2.1:-34189239	MS.gene071357:CDS
AGGAAATATTTGTTCATGAA+AGG	0.477898	2.1:-34188873	MS.gene071357:CDS
CCATCAATGATCGTGCAATT+GGG	0.483539	2.1:-34189137	MS.gene071357:CDS
GTGAGTTGTCTTACTTGGAA+AGG	0.487957	2.1:-34188975	MS.gene071357:CDS
GCTTGAAGCTTTAGTTGATG+TGG	0.488968	2.1:-34188953	MS.gene071357:CDS
TTCTGACCTTGATGATCTCA+TGG	0.490514	2.1:-34189280	MS.gene071357:CDS
ATCAAGGTCAGAAAGATTAA+GGG	0.492658	2.1:+34189290	None:intergenic
TAGTTGATGTGGAAAATGTT+GGG	0.494202	2.1:-34188942	MS.gene071357:CDS
TCGTTCCTTGATTTATCACC+AGG	0.504570	2.1:+34189090	None:intergenic
GTTCACCTGGTGATAAATCA+AGG	0.507986	2.1:-34189095	MS.gene071357:CDS
TTAGTTGATGTGGAAAATGT+TGG	0.523001	2.1:-34188943	MS.gene071357:CDS
AGGAACGAATGTGCAGAACT+TGG	0.523520	2.1:-34189075	MS.gene071357:CDS
ACCTTGATGATCTCATGGTA+TGG	0.538119	2.1:-34189275	MS.gene071357:CDS
TGTTGTGAAACAAGTGGTTA+AGG	0.541729	2.1:-34189007	MS.gene071357:CDS
TCTCTATCCTCAAGTTCACC+TGG	0.545696	2.1:-34189108	MS.gene071357:CDS
CTCTACACCAGCAAGGATGA+TGG	0.559987	2.1:-34189213	MS.gene071357:CDS
CTCTAAATATTGGGGTAAAG+GGG	0.564900	2.1:-34189040	MS.gene071357:CDS
GAGTTCCCAAGAGCAGAACT+TGG	0.571214	2.1:+34189233	None:intergenic
TTTATCACCAGGTGAACTTG+AGG	0.579933	2.1:+34189101	None:intergenic
ATTCTCTCTTCTTTGTCACG+TGG	0.580393	2.1:+34189339	None:intergenic
GGTCAGAAAGATTAAGGGGT+CGG	0.585362	2.1:+34189295	None:intergenic
GTTCTAGGCTCTAAATATTG+GGG	0.619040	2.1:-34189048	MS.gene071357:CDS
TCCATACCATGAGATCATCA+AGG	0.632686	2.1:+34189274	None:intergenic
TCAAGGTCAGAAAGATTAAG+GGG	0.633672	2.1:+34189291	None:intergenic
TTGGGAACTCTACACCAGCA+AGG	0.679749	2.1:-34189220	MS.gene071357:CDS
CAAGTGTGTTGTGAAACAAG+TGG	0.740021	2.1:-34189013	MS.gene071357:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGGAAATATTTGTTCATGAA+AGG	-	chr2.1:34189357-34189376	MS.gene071357:CDS	25.0%
!	ATATAGCTTCAAAGTTTCTA+TGG	-	chr2.1:34189057-34189076	MS.gene071357:CDS	25.0%
	ATCAAGGTCAGAAAGATTAA+GGG	+	chr2.1:34188943-34188962	None:intergenic	30.0%
!	AAAGTTGGTTTCCAAAAAGA+GGG	-	chr2.1:34189327-34189346	MS.gene071357:CDS	30.0%
!	TAGTTGATGTGGAAAATGTT+GGG	-	chr2.1:34189288-34189307	MS.gene071357:CDS	30.0%
!	TTAGTTGATGTGGAAAATGT+TGG	-	chr2.1:34189287-34189306	MS.gene071357:CDS	30.0%
!!	ATGTTCTAGGCTCTAAATAT+TGG	-	chr2.1:34189180-34189199	MS.gene071357:CDS	30.0%
!!	TGTTCTAGGCTCTAAATATT+GGG	-	chr2.1:34189181-34189200	MS.gene071357:CDS	30.0%
	ATTCTGTGAGTTGTCTTACT+TGG	-	chr2.1:34189250-34189269	MS.gene071357:CDS	35.0%
	CATCAAGGTCAGAAAGATTA+AGG	+	chr2.1:34188944-34188963	None:intergenic	35.0%
	CTCTAAATATTGGGGTAAAG+GGG	-	chr2.1:34189190-34189209	MS.gene071357:CDS	35.0%
	TCAAGGTCAGAAAGATTAAG+GGG	+	chr2.1:34188942-34188961	None:intergenic	35.0%
	TGTTGTGAAACAAGTGGTTA+AGG	-	chr2.1:34189223-34189242	MS.gene071357:CDS	35.0%
!	GAAAGTTGGTTTCCAAAAAG+AGG	-	chr2.1:34189326-34189345	MS.gene071357:CDS	35.0%
!	GCTCTAAATATTGGGGTAAA+GGG	-	chr2.1:34189189-34189208	MS.gene071357:CDS	35.0%
!!	GTTCTAGGCTCTAAATATTG+GGG	-	chr2.1:34189182-34189201	MS.gene071357:CDS	35.0%
	ATTCTCTCTTCTTTGTCACG+TGG	+	chr2.1:34188894-34188913	None:intergenic	40.0%
	CAAGTGTGTTGTGAAACAAG+TGG	-	chr2.1:34189217-34189236	MS.gene071357:CDS	40.0%
	CACTTGTTTCACAACACACT+TGG	+	chr2.1:34189219-34189238	None:intergenic	40.0%
	CCATCAATGATCGTGCAATT+GGG	-	chr2.1:34189093-34189112	MS.gene071357:CDS	40.0%
	GTGAGTTGTCTTACTTGGAA+AGG	-	chr2.1:34189255-34189274	MS.gene071357:CDS	40.0%
	GTTCACCTGGTGATAAATCA+AGG	-	chr2.1:34189135-34189154	MS.gene071357:CDS	40.0%
	TCCATACCATGAGATCATCA+AGG	+	chr2.1:34188959-34188978	None:intergenic	40.0%
	TCCATCAATGATCGTGCAAT+TGG	-	chr2.1:34189092-34189111	MS.gene071357:CDS	40.0%
	TCGTTCCTTGATTTATCACC+AGG	+	chr2.1:34189143-34189162	None:intergenic	40.0%
	TTTATCACCAGGTGAACTTG+AGG	+	chr2.1:34189132-34189151	None:intergenic	40.0%
!	AATGTTGGGTCTCAGAGTTT+TGG	-	chr2.1:34189302-34189321	MS.gene071357:CDS	40.0%
!	ACCTTGATGATCTCATGGTA+TGG	-	chr2.1:34188955-34188974	MS.gene071357:CDS	40.0%
!	GCTTGAAGCTTTAGTTGATG+TGG	-	chr2.1:34189277-34189296	MS.gene071357:CDS	40.0%
!	TTCTGACCTTGATGATCTCA+TGG	-	chr2.1:34188950-34188969	MS.gene071357:CDS	40.0%
!!	GGCTCTAAATATTGGGGTAA+AGG	-	chr2.1:34189188-34189207	MS.gene071357:CDS	40.0%
!!!	CTCAGAGTTTTGGAGAAAGT+TGG	-	chr2.1:34189312-34189331	MS.gene071357:CDS	40.0%
	AAGTTGCCAAGTTCTGCTCT+TGG	-	chr2.1:34188991-34189010	MS.gene071357:CDS	45.0%
	AGGAACGAATGTGCAGAACT+TGG	-	chr2.1:34189155-34189174	MS.gene071357:CDS	45.0%
	AGTTGCCAAGTTCTGCTCTT+GGG	-	chr2.1:34188992-34189011	MS.gene071357:CDS	45.0%
	CCCAATTGCACGATCATTGA+TGG	+	chr2.1:34189096-34189115	None:intergenic	45.0%
	CTCTTCTTTGTCACGTGGTT+TGG	+	chr2.1:34188889-34188908	None:intergenic	45.0%
	GCAGAACTTGGCTATGTTCT+AGG	-	chr2.1:34189167-34189186	MS.gene071357:CDS	45.0%
	GGTCAGAAAGATTAAGGGGT+CGG	+	chr2.1:34188938-34188957	None:intergenic	45.0%
	TCCAAAAAGAGGGAGTTCTG+AGG	-	chr2.1:34189337-34189356	MS.gene071357:CDS	45.0%
	TCTCTATCCTCAAGTTCACC+TGG	-	chr2.1:34189122-34189141	MS.gene071357:CDS	45.0%
!	TCCTCAGAACTCCCTCTTTT+TGG	+	chr2.1:34189341-34189360	None:intergenic	45.0%
	AAGGGGTCGGAGTGTGATTT+GGG	+	chr2.1:34188925-34188944	None:intergenic	50.0%
	CTCTACACCAGCAAGGATGA+TGG	-	chr2.1:34189017-34189036	MS.gene071357:CDS	50.0%
	GAGTTCCCAAGAGCAGAACT+TGG	+	chr2.1:34189000-34189019	None:intergenic	50.0%
	GTTGATGCCATCATCCTTGC+TGG	+	chr2.1:34189027-34189046	None:intergenic	50.0%
	TAAGGGGTCGGAGTGTGATT+TGG	+	chr2.1:34188926-34188945	None:intergenic	50.0%
	TTGGGAACTCTACACCAGCA+AGG	-	chr2.1:34189010-34189029	MS.gene071357:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	34188864	34189388	34188864	ID=MS.gene071357
chr2.1	mRNA	34188864	34189388	34188864	ID=MS.gene071357.t1;Parent=MS.gene071357
chr2.1	exon	34188864	34189388	34188864	ID=MS.gene071357.t1.exon1;Parent=MS.gene071357.t1
chr2.1	CDS	34188864	34189388	34188864	ID=cds.MS.gene071357.t1;Parent=MS.gene071357.t1

Gene Sequence

>MS.gene071357

ATGGAGATAACTTCTATTTCTTCCAAACCACGTGACAAAGAAGAGAGAATTGATCTAACCCAAATCACACTCCGACCCCTTAATCTTTCTGACCTTGATGATCTCATGGTATGGACAACTGATGAAAAAGTTGCCAAGTTCTGCTCTTGGGAACTCTACACCAGCAAGGATGATGGCATCAACTTCATTGAAAATATAGCTTCAAAGTTTCTATGGTGCAAAGCAATATCCATCAATGATCGTGCAATTGGGTGTGTTTCTCTATCCTCAAGTTCACCTGGTGATAAATCAAGGAACGAATGTGCAGAACTTGGCTATGTTCTAGGCTCTAAATATTGGGGTAAAGGGGTTGCCAAGTGTGTTGTGAAACAAGTGGTTAAGGCTGCATTCTGTGAGTTGTCTTACTTGGAAAGGCTTGAAGCTTTAGTTGATGTGGAAAATGTTGGGTCTCAGAGTTTTGGAGAAAGTTGGTTTCCAAAAAGAGGGAGTTCTGAGGAAATATTTGTTCATGAAAGGAAAAAGTAG

Protein sequence

>MS.gene071357.t1

MEITSISSKPRDKEERIDLTQITLRPLNLSDLDDLMVWTTDEKVAKFCSWELYTSKDDGINFIENIASKFLWCKAISINDRAIGCVSLSSSSPGDKSRNECAELGYVLGSKYWGKGVAKCVVKQVVKAAFCELSYLERLEALVDVENVGSQSFGESWFPKRGSSEEIFVHERKK