AlfalfaGEDB | MS.gene001032

M. sativa cultivar XinJiangDaYe / MS.gene001032

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001032.t1	XP_003610737.1	93.2	190	13	0	1	190	1	190	2.80E-93	351.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001032.t1	P05332	31.8	179	111	3	15	187	2	175	3.8e-14	79.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001032.t1	G7K3P2	93.2	190	13	0	1	190	1	190	2.0e-93	351.3

TFs/TRs:

Gene ID	Type	Classification
MS.gene001032.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene74082	MS.gene001032	PPI
MS.gene49470	MS.gene001032	PPI
MS.gene001037	MS.gene001032	PPI
MS.gene070944	MS.gene001032	PPI
MS.gene001033	MS.gene001032	PPI
MS.gene001032	MS.gene070944	PPI
MS.gene21827	MS.gene001032	PPI
MS.gene024854	MS.gene001032	PPI
MS.gene006417	MS.gene001032	PPI
MS.gene49471	MS.gene001032	PPI
MS.gene001032	MS.gene024857	PPI
MS.gene071361	MS.gene001032	PPI
MS.gene49468	MS.gene001032	PPI
MS.gene21828	MS.gene001032	PPI
MS.gene95802	MS.gene001032	PPI
MS.gene34370	MS.gene001032	PPI
MS.gene001032	MS.gene071357	PPI
MS.gene49472	MS.gene001032	PPI
MS.gene77154	MS.gene001032	PPI
MS.gene043813	MS.gene001032	PPI
MS.gene34371	MS.gene001032	PPI
MS.gene001032	MS.gene04530	PPI
MS.gene001038	MS.gene001032	PPI
MS.gene070945	MS.gene001032	PPI
MS.gene21830	MS.gene001032	PPI
MS.gene21833	MS.gene001032	PPI
MS.gene043815	MS.gene001032	PPI
MS.gene001034	MS.gene001032	PPI
MS.gene21831	MS.gene001032	PPI
MS.gene95801	MS.gene001032	PPI
MS.gene024614	MS.gene001032	PPI
MS.gene29937	MS.gene001032	PPI
MS.gene024858	MS.gene001032	PPI
MS.gene024859	MS.gene001032	PPI
MS.gene024855	MS.gene001032	PPI
MS.gene006418	MS.gene001032	PPI
MS.gene024612	MS.gene001032	PPI
MS.gene050493	MS.gene001032	PPI
MS.gene001032	MS.gene22888	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001032.t1	MTR_5g006450	93.158	190	13	0	1	190	1	190	1.74e-128	359
MS.gene001032.t1	MTR_5g006470	76.842	190	42	1	1	190	1	188	2.76e-101	290
MS.gene001032.t1	MTR_5g006520	77.895	190	42	0	1	190	1719	1908	1.31e-97	310
MS.gene001032.t1	MTR_5g006520	50.667	75	14	1	97	171	1655	1706	6.22e-12	63.9
MS.gene001032.t1	MTR_5g006510	73.298	191	44	3	1	190	7	191	1.50e-94	273
MS.gene001032.t1	MTR_5g006460	72.821	195	46	5	1	190	1	193	7.78e-93	269
MS.gene001032.t1	MTR_1g103110	63.542	192	66	2	1	190	1	190	2.82e-84	247
MS.gene001032.t1	MTR_1g103150	59.375	192	74	2	1	190	1	190	2.35e-78	232
MS.gene001032.t1	MTR_7g114410	41.279	172	98	1	16	187	3	171	2.96e-45	147
MS.gene001032.t1	MTR_3g463610	39.181	171	104	0	17	187	3	173	8.70e-44	145
MS.gene001032.t1	MTR_7g114410	40.764	157	90	1	16	172	3	156	2.87e-41	137
MS.gene001032.t1	MTR_7g024760	40.476	168	95	3	20	187	7	169	1.15e-36	125
MS.gene001032.t1	MTR_7g024780	38.012	171	102	2	17	187	4	170	1.93e-35	122
MS.gene001032.t1	MTR_7g024765	38.095	168	99	3	20	187	7	169	6.38e-35	121
MS.gene001032.t1	MTR_1g103320	55.128	78	33	1	53	130	13	88	1.93e-20	82.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001032.t1	AT2G32030	51.190	168	75	3	21	187	24	185	6.68e-58	180
MS.gene001032.t1	AT2G32020	50.595	168	76	2	21	187	19	180	1.04e-53	169
MS.gene001032.t1	AT3G22560	37.647	170	101	2	20	187	6	172	9.13e-35	121

Find 48 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGTTCTAGGCTCTAAATAT+TGG	0.292142	5.4:-2206754	MS.gene001032:CDS
TCCTTAGAACTCCCTCCTTT+TGG	0.297796	5.4:+2206594	None:intergenic
GCAGAACTTGGCTATGTTCT+AGG	0.302641	5.4:-2206767	MS.gene001032:CDS
TGTTCTAGGCTCTAAATATT+GGG	0.316974	5.4:-2206753	MS.gene001032:CDS
GAGTTCCCAAGAGCAGAATT+TGG	0.329740	5.4:+2206937	None:intergenic
GAAGGGTTCGAAGTGTGATT+TGG	0.336631	5.4:+2207011	None:intergenic
AAGGGTTCGAAGTGTGATTT+GGG	0.345813	5.4:+2207012	None:intergenic
GCTCTAAATATTGGGGTAAA+GGG	0.356917	5.4:-2206745	MS.gene001032:CDS
AAGTTCTGCACATTTGTTCC+TGG	0.375848	5.4:+2206781	None:intergenic
CTAAACAATTGAATTATAGT+TGG	0.392086	5.4:+2206507	None:intergenic
ATATAGCTACCAAGTTTCTA+TGG	0.402105	5.4:-2206877	MS.gene001032:CDS
TGCATCAATGATCGTGCAAT+TGG	0.403589	5.4:-2206842	MS.gene001032:CDS
TCCAAAAGGAGGGAGTTCTA+AGG	0.410439	5.4:-2206595	MS.gene001032:CDS
GGCTCTAAATATTGGGGTAA+AGG	0.422886	5.4:-2206746	MS.gene001032:CDS
AAGTGGCCAAATTCTGCTCT+TGG	0.423997	5.4:-2206943	MS.gene001032:CDS
GTTGATGCCATCATCCTTGC+TGG	0.429573	5.4:+2206910	None:intergenic
ATTCTGTGAGTTGTCTTACT+TGG	0.446468	5.4:-2206684	MS.gene001032:CDS
AGTGGCCAAATTCTGCTCTT+GGG	0.474265	5.4:-2206942	MS.gene001032:CDS
AGGAACAAATGTGCAGAACT+TGG	0.475881	5.4:-2206779	MS.gene001032:CDS
TGCTGGGTCTCAAAGAGTGC+TGG	0.477108	5.4:-2206630	MS.gene001032:CDS
CATCAAGGTCAGAAAGTTGA+AGG	0.479974	5.4:+2206993	None:intergenic
AGGAAATATTTGTTCATGAA+AGG	0.480337	5.4:-2206575	MS.gene001032:CDS
GTGAGTTGTCTTACTTGGAA+AGG	0.487957	5.4:-2206679	MS.gene001032:CDS
GCTTGAAGCTTTAGTTGATG+TGG	0.488968	5.4:-2206657	MS.gene001032:CDS
TGCTTTGCACCATAGAAACT+TGG	0.496119	5.4:+2206868	None:intergenic
TTCTGACCTTGATGATCTCA+TGG	0.505164	5.4:-2206984	MS.gene001032:CDS
TGTTGTGAAACAAGTTGTTA+AGG	0.509712	5.4:-2206711	MS.gene001032:CDS
TTGTTCCTGGATTTATCACC+AGG	0.514781	5.4:+2206794	None:intergenic
TTAGTTGATGTGGAAAATGC+TGG	0.527178	5.4:-2206647	MS.gene001032:CDS
GGATAAAGCTGGTTTCCAAA+AGG	0.538652	5.4:-2206609	MS.gene001032:CDS
GTTCACCTGGTGATAAATCC+AGG	0.542743	5.4:-2206799	MS.gene001032:CDS
TCTCTATCCTCAAGTTCACC+TGG	0.545696	5.4:-2206812	MS.gene001032:CDS
CAAAGAGTGCTGGATAAAGC+TGG	0.554974	5.4:-2206620	MS.gene001032:CDS
CTCTACACCAGCAAGGATGA+TGG	0.559987	5.4:-2206917	MS.gene001032:CDS
CTCTAAATATTGGGGTAAAG+GGG	0.564900	5.4:-2206744	MS.gene001032:CDS
GCATCAATGATCGTGCAATT+GGG	0.567462	5.4:-2206841	MS.gene001032:CDS
TTTATCACCAGGTGAACTTG+AGG	0.573291	5.4:+2206805	None:intergenic
TAGTTGATGTGGAAAATGCT+GGG	0.573363	5.4:-2206646	MS.gene001032:CDS
TAAAGCTGGTTTCCAAAAGG+AGG	0.595377	5.4:-2206606	MS.gene001032:CDS
ACCTTGATGATCTCATGGTG+TGG	0.606125	5.4:-2206979	MS.gene001032:CDS
ATTCTCTCTTCTTTGCTACG+TGG	0.606655	5.4:+2207043	None:intergenic
AACTTGTTTCACAACACATG+TGG	0.607173	5.4:+2206718	None:intergenic
GTTCTAGGCTCTAAATATTG+GGG	0.619040	5.4:-2206752	MS.gene001032:CDS
ATCAAGGTCAGAAAGTTGAA+GGG	0.644369	5.4:+2206994	None:intergenic
TCCACACCATGAGATCATCA+AGG	0.646997	5.4:+2206978	None:intergenic
GAGATCAGTAGAAAGAACAC+TGG	0.658551	5.4:+2206529	None:intergenic
AAAGCTGGTTTCCAAAAGGA+GGG	0.662462	5.4:-2206605	MS.gene001032:CDS
TTGGGAACTCTACACCAGCA+AGG	0.679749	5.4:-2206924	MS.gene001032:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGGAAATATTTGTTCATGAA+AGG	-	chr5.4:2207015-2207034	MS.gene001032:CDS	25.0%
	ATATAGCTACCAAGTTTCTA+TGG	-	chr5.4:2206713-2206732	MS.gene001032:CDS	30.0%
	TGTTGTGAAACAAGTTGTTA+AGG	-	chr5.4:2206879-2206898	MS.gene001032:CDS	30.0%
!!	ATGTTCTAGGCTCTAAATAT+TGG	-	chr5.4:2206836-2206855	MS.gene001032:CDS	30.0%
!!	TGTTCTAGGCTCTAAATATT+GGG	-	chr5.4:2206837-2206856	MS.gene001032:CDS	30.0%
	ATCAAGGTCAGAAAGTTGAA+GGG	+	chr5.4:2206599-2206618	None:intergenic	35.0%
	ATTCTGTGAGTTGTCTTACT+TGG	-	chr5.4:2206906-2206925	MS.gene001032:CDS	35.0%
	CTCTAAATATTGGGGTAAAG+GGG	-	chr5.4:2206846-2206865	MS.gene001032:CDS	35.0%
!	AACTTGTTTCACAACACATG+TGG	+	chr5.4:2206875-2206894	None:intergenic	35.0%
!	GCTCTAAATATTGGGGTAAA+GGG	-	chr5.4:2206845-2206864	MS.gene001032:CDS	35.0%
!	TAGTTGATGTGGAAAATGCT+GGG	-	chr5.4:2206944-2206963	MS.gene001032:CDS	35.0%
!	TTAGTTGATGTGGAAAATGC+TGG	-	chr5.4:2206943-2206962	MS.gene001032:CDS	35.0%
!!	GTTCTAGGCTCTAAATATTG+GGG	-	chr5.4:2206838-2206857	MS.gene001032:CDS	35.0%
	AAGGGTTCGAAGTGTGATTT+GGG	+	chr5.4:2206581-2206600	None:intergenic	40.0%
	AAGTTCTGCACATTTGTTCC+TGG	+	chr5.4:2206812-2206831	None:intergenic	40.0%
	AGGAACAAATGTGCAGAACT+TGG	-	chr5.4:2206811-2206830	MS.gene001032:CDS	40.0%
	ATTCTCTCTTCTTTGCTACG+TGG	+	chr5.4:2206550-2206569	None:intergenic	40.0%
	CATCAAGGTCAGAAAGTTGA+AGG	+	chr5.4:2206600-2206619	None:intergenic	40.0%
	GAGATCAGTAGAAAGAACAC+TGG	+	chr5.4:2207064-2207083	None:intergenic	40.0%
	GCATCAATGATCGTGCAATT+GGG	-	chr5.4:2206749-2206768	MS.gene001032:CDS	40.0%
	GTGAGTTGTCTTACTTGGAA+AGG	-	chr5.4:2206911-2206930	MS.gene001032:CDS	40.0%
	GTGGACAACTGATGAAAAAG+TGG	-	chr5.4:2206630-2206649	MS.gene001032:CDS	40.0%
	TGCATCAATGATCGTGCAAT+TGG	-	chr5.4:2206748-2206767	MS.gene001032:CDS	40.0%
	TGCTTTGCACCATAGAAACT+TGG	+	chr5.4:2206725-2206744	None:intergenic	40.0%
	TTGTTCCTGGATTTATCACC+AGG	+	chr5.4:2206799-2206818	None:intergenic	40.0%
	TTTATCACCAGGTGAACTTG+AGG	+	chr5.4:2206788-2206807	None:intergenic	40.0%
!	AAAGCTGGTTTCCAAAAGGA+GGG	-	chr5.4:2206985-2207004	MS.gene001032:CDS	40.0%
!	GCTTGAAGCTTTAGTTGATG+TGG	-	chr5.4:2206933-2206952	MS.gene001032:CDS	40.0%
!	GGATAAAGCTGGTTTCCAAA+AGG	-	chr5.4:2206981-2207000	MS.gene001032:CDS	40.0%
!	TAAAGCTGGTTTCCAAAAGG+AGG	-	chr5.4:2206984-2207003	MS.gene001032:CDS	40.0%
!	TTCTGACCTTGATGATCTCA+TGG	-	chr5.4:2206606-2206625	MS.gene001032:CDS	40.0%
!!	GGCTCTAAATATTGGGGTAA+AGG	-	chr5.4:2206844-2206863	MS.gene001032:CDS	40.0%
	AAGTGGCCAAATTCTGCTCT+TGG	-	chr5.4:2206647-2206666	MS.gene001032:CDS	45.0%
	AGTGGCCAAATTCTGCTCTT+GGG	-	chr5.4:2206648-2206667	MS.gene001032:CDS	45.0%
	GAAGGGTTCGAAGTGTGATT+TGG	+	chr5.4:2206582-2206601	None:intergenic	45.0%
	GAGTTCCCAAGAGCAGAATT+TGG	+	chr5.4:2206656-2206675	None:intergenic	45.0%
	GCAGAACTTGGCTATGTTCT+AGG	-	chr5.4:2206823-2206842	MS.gene001032:CDS	45.0%
	GTTCACCTGGTGATAAATCC+AGG	-	chr5.4:2206791-2206810	MS.gene001032:CDS	45.0%
	TCCAAAAGGAGGGAGTTCTA+AGG	-	chr5.4:2206995-2207014	MS.gene001032:CDS	45.0%
	TCCACACCATGAGATCATCA+AGG	+	chr5.4:2206615-2206634	None:intergenic	45.0%
	TCTCTATCCTCAAGTTCACC+TGG	-	chr5.4:2206778-2206797	MS.gene001032:CDS	45.0%
!	ACCTTGATGATCTCATGGTG+TGG	-	chr5.4:2206611-2206630	MS.gene001032:CDS	45.0%
!	TCCTTAGAACTCCCTCCTTT+TGG	+	chr5.4:2206999-2207018	None:intergenic	45.0%
!!	CAAAGAGTGCTGGATAAAGC+TGG	-	chr5.4:2206970-2206989	MS.gene001032:CDS	45.0%
	CTCTACACCAGCAAGGATGA+TGG	-	chr5.4:2206673-2206692	MS.gene001032:CDS	50.0%
	GTTGATGCCATCATCCTTGC+TGG	+	chr5.4:2206683-2206702	None:intergenic	50.0%
	TTGGGAACTCTACACCAGCA+AGG	-	chr5.4:2206666-2206685	MS.gene001032:CDS	50.0%
!	TGCTGGGTCTCAAAGAGTGC+TGG	-	chr5.4:2206960-2206979	MS.gene001032:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	2206520	2207092	2206520	ID=MS.gene001032
chr5.4	mRNA	2206520	2207092	2206520	ID=MS.gene001032.t1;Parent=MS.gene001032
chr5.4	exon	2206520	2207092	2206520	ID=MS.gene001032.t1.exon1;Parent=MS.gene001032.t1
chr5.4	CDS	2206520	2207092	2206520	ID=cds.MS.gene001032.t1;Parent=MS.gene001032.t1

Gene Sequence

>MS.gene001032

ATGGAGAGAACTTCTATTTCTTTCGAACCACGTAGCAAAGAAGAGAGAATTGATTTAACCCAAATCACACTTCGAACCCTTCAACTTTCTGACCTTGATGATCTCATGGTGTGGACAACTGATGAAAAAGTGGCCAAATTCTGCTCTTGGGAACTCTACACCAGCAAGGATGATGGCATCAACTTCATTGAAAATATAGCTACCAAGTTTCTATGGTGCAAAGCAATATGCATCAATGATCGTGCAATTGGGTGTGTTTCTCTATCCTCAAGTTCACCTGGTGATAAATCCAGGAACAAATGTGCAGAACTTGGCTATGTTCTAGGCTCTAAATATTGGGGTAAAGGGGTTGCCACATGTGTTGTGAAACAAGTTGTTAAGGTTGCATTCTGTGAGTTGTCTTACTTGGAAAGGCTTGAAGCTTTAGTTGATGTGGAAAATGCTGGGTCTCAAAGAGTGCTGGATAAAGCTGGTTTCCAAAAGGAGGGAGTTCTAAGGAAATATTTGTTCATGAAAGGAAAAAGTAGAGATATGATCATGTCCAGTGTTCTTTCTACTGATCTCCAACTATAA

Protein sequence

>MS.gene001032.t1

MERTSISFEPRSKEERIDLTQITLRTLQLSDLDDLMVWTTDEKVAKFCSWELYTSKDDGINFIENIATKFLWCKAICINDRAIGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVATCVVKQVVKVAFCELSYLERLEALVDVENAGSQRVLDKAGFQKEGVLRKYLFMKGKSRDMIMSSVLSTDLQL