AlfalfaGEDB | MS.gene043813

M. sativa cultivar XinJiangDaYe / MS.gene043813

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043813.t1	XP_003610737.1	84.5	174	26	1	1	173	1	174	4.20E-75	290.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043813.t1	G7K3P2	84.5	174	26	1	1	173	1	174	3.0e-75	290.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene043813.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene77154	MS.gene043813	PPI
MS.gene043813	MS.gene04530	PPI
MS.gene34371	MS.gene043813	PPI
MS.gene043813	MS.gene024614	PPI
MS.gene043813	MS.gene21831	PPI
MS.gene070945	MS.gene043813	PPI
MS.gene21833	MS.gene043813	PPI
MS.gene043815	MS.gene043813	PPI
MS.gene21828	MS.gene043813	PPI
MS.gene95802	MS.gene043813	PPI
MS.gene49472	MS.gene043813	PPI
MS.gene024859	MS.gene043813	PPI
MS.gene024855	MS.gene043813	PPI
MS.gene043813	MS.gene070944	PPI
MS.gene024612	MS.gene043813	PPI
MS.gene001038	MS.gene043813	PPI
MS.gene043813	MS.gene49470	PPI
MS.gene21830	MS.gene043813	PPI
MS.gene95801	MS.gene043813	PPI
MS.gene043813	MS.gene74082	PPI
MS.gene29937	MS.gene043813	PPI
MS.gene024858	MS.gene043813	PPI
MS.gene043813	MS.gene024857	PPI
MS.gene006418	MS.gene043813	PPI
MS.gene043813	MS.gene95801	PPI
MS.gene050493	MS.gene043813	PPI
MS.gene21827	MS.gene043813	PPI
MS.gene024854	MS.gene043813	PPI
MS.gene043813	MS.gene071357	PPI
MS.gene043813	MS.gene001032	PPI
MS.gene001037	MS.gene043813	PPI
MS.gene043813	MS.gene49471	PPI
MS.gene006417	MS.gene043813	PPI
MS.gene001033	MS.gene043813	PPI
MS.gene071361	MS.gene043813	PPI
MS.gene49468	MS.gene043813	PPI
MS.gene043813	MS.gene001034	PPI
MS.gene34370	MS.gene043813	PPI
MS.gene043813	MS.gene22888	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043813.t1	MTR_5g006450	84.483	174	26	1	1	173	1	174	5.64e-103	294
MS.gene043813.t1	MTR_5g006470	68.966	174	51	2	1	173	1	172	7.15e-76	225
MS.gene043813.t1	MTR_5g006520	71.951	164	45	1	1	163	1719	1882	5.37e-74	241
MS.gene043813.t1	MTR_5g006510	68.293	164	44	4	1	162	7	164	2.79e-68	206
MS.gene043813.t1	MTR_5g006460	68.452	168	45	6	1	162	1	166	2.66e-66	201
MS.gene043813.t1	MTR_1g103110	59.394	165	62	3	1	162	1	163	1.33e-60	186
MS.gene043813.t1	MTR_1g103150	56.970	165	66	3	1	162	1	163	2.35e-57	178
MS.gene043813.t1	MTR_7g114410	35.294	136	85	1	16	151	3	135	3.19e-27	100
MS.gene043813.t1	MTR_7g114410	35.294	136	85	1	16	151	3	135	7.15e-27	100
MS.gene043813.t1	MTR_3g463610	33.813	139	92	0	17	155	3	141	1.42e-26	100
MS.gene043813.t1	MTR_7g024765	32.026	153	98	3	20	171	7	154	2.80e-22	88.6
MS.gene043813.t1	MTR_1g103320	42.857	119	51	3	53	170	13	115	5.73e-22	85.9
MS.gene043813.t1	MTR_7g024760	36.765	136	81	3	20	155	7	137	1.07e-21	87.0
MS.gene043813.t1	MTR_7g024780	32.026	153	99	3	17	168	4	152	1.75e-20	83.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043813.t1	AT2G32030	46.104	154	68	4	3	155	14	153	1.22e-39	133
MS.gene043813.t1	AT2G32020	47.794	136	64	2	21	155	19	148	6.21e-36	123
MS.gene043813.t1	AT3G22560	33.793	145	90	3	21	162	7	148	1.14e-20	84.3

Find 42 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCAAGGTCAGAAAGATTA+AGG	0.227074	2.2:+27862183	None:intergenic
CTCTTCTTTGTCACGTGGTT+TGG	0.270188	2.2:+27862238	None:intergenic
ATGTTCTAGGCTCTAAATAT+TGG	0.292142	2.2:-27861944	MS.gene043813:CDS
GCAGAACTTGGCTATGTTCT+AGG	0.302641	2.2:-27861957	MS.gene043813:CDS
TAAGGGGTCGGAGTGTGATT+TGG	0.307480	2.2:+27862201	None:intergenic
TGTTCTAGGCTCTAAATATT+GGG	0.316974	2.2:-27861943	MS.gene043813:CDS
AAGGGGTCGGAGTGTGATTT+GGG	0.320072	2.2:+27862202	None:intergenic
ATATAGCTTCAAAGTTTCTA+TGG	0.332117	2.2:-27862067	MS.gene043813:CDS
CCCAATTGCACGATCATTGA+TGG	0.348489	2.2:+27862031	None:intergenic
AATGTTGGGTCTCAGAGTTT+TGG	0.354229	2.2:-27861822	MS.gene043813:CDS
GCTCTAAATATTGGGGTAAA+GGG	0.356917	2.2:-27861935	MS.gene043813:CDS
AAGTTGCCAAGTTCTGCTTT+TGG	0.388707	2.2:-27862133	MS.gene043813:CDS
TCCATCAATGATCGTGCAAT+TGG	0.408524	2.2:-27862032	MS.gene043813:CDS
GGCTCTAAATATTGGGGTAA+AGG	0.422886	2.2:-27861936	MS.gene043813:CDS
GTTGATGCCATCATCCTTGC+TGG	0.429573	2.2:+27862100	None:intergenic
ATTCTGTGAGTTGTCTTACT+TGG	0.446468	2.2:-27861874	MS.gene043813:CDS
GTGAGTTGTCTTACTTGGAA+AGG	0.487957	2.2:-27861869	MS.gene043813:CDS
GCTTGAAGCTTTAGTTGATG+TGG	0.488968	2.2:-27861847	MS.gene043813:CDS
TTCTGACCTTGATGATCTCA+TGG	0.490514	2.2:-27862174	MS.gene043813:CDS
ATCAAGGTCAGAAAGATTAA+GGG	0.492658	2.2:+27862184	None:intergenic
TAGTTGATGTGGAAAATGTT+GGG	0.494202	2.2:-27861836	MS.gene043813:CDS
TCGTTCCTTGATTTATCACC+AGG	0.504570	2.2:+27861984	None:intergenic
AGGAAATATTTGTTCATGAA+CGG	0.507359	2.2:-27861767	MS.gene043813:CDS
GTTCACCTGGTGATAAATCA+AGG	0.507986	2.2:-27861989	MS.gene043813:CDS
TTAGTTGATGTGGAAAATGT+TGG	0.523001	2.2:-27861837	MS.gene043813:CDS
AGGAACGAATGTGCAGAACT+TGG	0.523520	2.2:-27861969	MS.gene043813:CDS
GAGTTCCCAAAAGCAGAACT+TGG	0.536427	2.2:+27862127	None:intergenic
ACCTTGATGATCTCATGGTA+TGG	0.538119	2.2:-27862169	MS.gene043813:CDS
TCTCTATCCTCAAGTTCACC+TGG	0.545696	2.2:-27862002	MS.gene043813:CDS
CCATCAATGATCGTGCAATT+GGG	0.547158	2.2:-27862031	MS.gene043813:CDS
TGTTGTGAAACAAGTGGTTA+AGG	0.553508	2.2:-27861901	MS.gene043813:CDS
CTCTACACCAGCAAGGATGA+TGG	0.559987	2.2:-27862107	MS.gene043813:CDS
CTCTAAATATTGGGGTAAAG+GGG	0.564900	2.2:-27861934	MS.gene043813:CDS
TTTATCACCAGGTGAACTTG+AGG	0.579933	2.2:+27861995	None:intergenic
ATTCTCTCTTCTTTGTCACG+TGG	0.580393	2.2:+27862233	None:intergenic
GGTCAGAAAGATTAAGGGGT+CGG	0.585362	2.2:+27862189	None:intergenic
CACTTGTTTCACAACACACG+TGG	0.596860	2.2:+27861908	None:intergenic
GTTCTAGGCTCTAAATATTG+GGG	0.619040	2.2:-27861942	MS.gene043813:CDS
TCCATACCATGAGATCATCA+AGG	0.632686	2.2:+27862168	None:intergenic
TCAAGGTCAGAAAGATTAAG+GGG	0.633672	2.2:+27862185	None:intergenic
TTGGGAACTCTACACCAGCA+AGG	0.654912	2.2:-27862114	MS.gene043813:CDS
CACGTGTGTTGTGAAACAAG+TGG	0.736637	2.2:-27861907	MS.gene043813:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGGAAATATTTGTTCATGAA+CGG	-	chr2.2:27862251-27862270	MS.gene043813:CDS	25.0%
!	ATATAGCTTCAAAGTTTCTA+TGG	-	chr2.2:27861951-27861970	MS.gene043813:CDS	25.0%
	ATCAAGGTCAGAAAGATTAA+GGG	+	chr2.2:27861837-27861856	None:intergenic	30.0%
!	TAGTTGATGTGGAAAATGTT+GGG	-	chr2.2:27862182-27862201	MS.gene043813:CDS	30.0%
!	TTAGTTGATGTGGAAAATGT+TGG	-	chr2.2:27862181-27862200	MS.gene043813:CDS	30.0%
!!	ATGTTCTAGGCTCTAAATAT+TGG	-	chr2.2:27862074-27862093	MS.gene043813:CDS	30.0%
!!	TGTTCTAGGCTCTAAATATT+GGG	-	chr2.2:27862075-27862094	MS.gene043813:CDS	30.0%
	ATTCTGTGAGTTGTCTTACT+TGG	-	chr2.2:27862144-27862163	MS.gene043813:CDS	35.0%
	CATCAAGGTCAGAAAGATTA+AGG	+	chr2.2:27861838-27861857	None:intergenic	35.0%
	CTCTAAATATTGGGGTAAAG+GGG	-	chr2.2:27862084-27862103	MS.gene043813:CDS	35.0%
	TCAAGGTCAGAAAGATTAAG+GGG	+	chr2.2:27861836-27861855	None:intergenic	35.0%
	TGTTGTGAAACAAGTGGTTA+AGG	-	chr2.2:27862117-27862136	MS.gene043813:CDS	35.0%
!	AAAGCTGGTTTCCAAAAAGA+GGG	-	chr2.2:27862221-27862240	MS.gene043813:CDS	35.0%
!	GCTCTAAATATTGGGGTAAA+GGG	-	chr2.2:27862083-27862102	MS.gene043813:CDS	35.0%
!!	GTTCTAGGCTCTAAATATTG+GGG	-	chr2.2:27862076-27862095	MS.gene043813:CDS	35.0%
	ATTCTCTCTTCTTTGTCACG+TGG	+	chr2.2:27861788-27861807	None:intergenic	40.0%
	CCATCAATGATCGTGCAATT+GGG	-	chr2.2:27861987-27862006	MS.gene043813:CDS	40.0%
	GTGAGTTGTCTTACTTGGAA+AGG	-	chr2.2:27862149-27862168	MS.gene043813:CDS	40.0%
	GTTCACCTGGTGATAAATCA+AGG	-	chr2.2:27862029-27862048	MS.gene043813:CDS	40.0%
	TCCAAAAAGAGGGAGTTCTA+AGG	-	chr2.2:27862231-27862250	MS.gene043813:CDS	40.0%
	TCCATACCATGAGATCATCA+AGG	+	chr2.2:27861853-27861872	None:intergenic	40.0%
	TCCATCAATGATCGTGCAAT+TGG	-	chr2.2:27861986-27862005	MS.gene043813:CDS	40.0%
	TCGTTCCTTGATTTATCACC+AGG	+	chr2.2:27862037-27862056	None:intergenic	40.0%
	TTTATCACCAGGTGAACTTG+AGG	+	chr2.2:27862026-27862045	None:intergenic	40.0%
!	AAGTTGCCAAGTTCTGCTTT+TGG	-	chr2.2:27861885-27861904	MS.gene043813:CDS	40.0%
!	AATGTTGGGTCTCAGAGTTT+TGG	-	chr2.2:27862196-27862215	MS.gene043813:CDS	40.0%
!	ACCTTGATGATCTCATGGTA+TGG	-	chr2.2:27861849-27861868	MS.gene043813:CDS	40.0%
!	GAAAGCTGGTTTCCAAAAAG+AGG	-	chr2.2:27862220-27862239	MS.gene043813:CDS	40.0%
!	GCTTGAAGCTTTAGTTGATG+TGG	-	chr2.2:27862171-27862190	MS.gene043813:CDS	40.0%
!	TCCTTAGAACTCCCTCTTTT+TGG	+	chr2.2:27862235-27862254	None:intergenic	40.0%
!	TTCTGACCTTGATGATCTCA+TGG	-	chr2.2:27861844-27861863	MS.gene043813:CDS	40.0%
!!	AGTTGCCAAGTTCTGCTTTT+GGG	-	chr2.2:27861886-27861905	MS.gene043813:CDS	40.0%
!!	GGCTCTAAATATTGGGGTAA+AGG	-	chr2.2:27862082-27862101	MS.gene043813:CDS	40.0%
	AGGAACGAATGTGCAGAACT+TGG	-	chr2.2:27862049-27862068	MS.gene043813:CDS	45.0%
	CACGTGTGTTGTGAAACAAG+TGG	-	chr2.2:27862111-27862130	MS.gene043813:CDS	45.0%
	CACTTGTTTCACAACACACG+TGG	+	chr2.2:27862113-27862132	None:intergenic	45.0%
	CCCAATTGCACGATCATTGA+TGG	+	chr2.2:27861990-27862009	None:intergenic	45.0%
	CTCTTCTTTGTCACGTGGTT+TGG	+	chr2.2:27861783-27861802	None:intergenic	45.0%
	GAGTTCCCAAAAGCAGAACT+TGG	+	chr2.2:27861894-27861913	None:intergenic	45.0%
	GCAGAACTTGGCTATGTTCT+AGG	-	chr2.2:27862061-27862080	MS.gene043813:CDS	45.0%
	GGTCAGAAAGATTAAGGGGT+CGG	+	chr2.2:27861832-27861851	None:intergenic	45.0%
	TCTCTATCCTCAAGTTCACC+TGG	-	chr2.2:27862016-27862035	MS.gene043813:CDS	45.0%
!!!	CTCAGAGTTTTGGAGAAAGC+TGG	-	chr2.2:27862206-27862225	MS.gene043813:CDS	45.0%
	AAGGGGTCGGAGTGTGATTT+GGG	+	chr2.2:27861819-27861838	None:intergenic	50.0%
	CTCTACACCAGCAAGGATGA+TGG	-	chr2.2:27861911-27861930	MS.gene043813:CDS	50.0%
	GTTGATGCCATCATCCTTGC+TGG	+	chr2.2:27861921-27861940	None:intergenic	50.0%
	TAAGGGGTCGGAGTGTGATT+TGG	+	chr2.2:27861820-27861839	None:intergenic	50.0%
	TTGGGAACTCTACACCAGCA+AGG	-	chr2.2:27861904-27861923	MS.gene043813:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	27861758	27862282	27861758	ID=MS.gene043813
chr2.2	mRNA	27861758	27862282	27861758	ID=MS.gene043813.t1;Parent=MS.gene043813
chr2.2	exon	27861758	27862282	27861758	ID=MS.gene043813.t1.exon1;Parent=MS.gene043813.t1
chr2.2	CDS	27861758	27862282	27861758	ID=cds.MS.gene043813.t1;Parent=MS.gene043813.t1

Gene Sequence

>MS.gene043813

ATGGAGATAACTTCTATTTCTTCCAAACCACGTGACAAAGAAGAGAGAATTGATCTAACCCAAATCACACTCCGACCCCTTAATCTTTCTGACCTTGATGATCTCATGGTATGGACAACTGATGAAAAAGTTGCCAAGTTCTGCTTTTGGGAACTCTACACCAGCAAGGATGATGGCATCAACTTCATTGAAAATATAGCTTCAAAGTTTCTATGGTGCAAAGCAATGTCCATCAATGATCGTGCAATTGGGTGTGTTTCTCTATCCTCAAGTTCACCTGGTGATAAATCAAGGAACGAATGTGCAGAACTTGGCTATGTTCTAGGCTCTAAATATTGGGGTAAAGGGGTTGCCACGTGTGTTGTGAAACAAGTGGTTAAGGCTGCATTCTGTGAGTTGTCTTACTTGGAAAGGCTTGAAGCTTTAGTTGATGTGGAAAATGTTGGGTCTCAGAGTTTTGGAGAAAGCTGGTTTCCAAAAAGAGGGAGTTCTAAGGAAATATTTGTTCATGAACGGAAAAAGTAG

Protein sequence

>MS.gene043813.t1

MEITSISSKPRDKEERIDLTQITLRPLNLSDLDDLMVWTTDEKVAKFCFWELYTSKDDGINFIENIASKFLWCKAMSINDRAIGCVSLSSSSPGDKSRNECAELGYVLGSKYWGKGVATCVVKQVVKAAFCELSYLERLEALVDVENVGSQSFGESWFPKRGSSKEIFVHERKK