AlfalfaGEDB | MS.gene001038

M. sativa cultivar XinJiangDaYe / MS.gene001038

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001038.t1	XP_024639963.1	92.6	190	14	0	1	190	1	190	2.30E-95	358.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001038.t1	P05332	31.8	173	107	3	21	187	8	175	4.1e-16	86.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001038.t1	G7K3P8	92.6	190	14	0	1	190	1719	1908	1.7e-95	358.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene001038.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene001038	MS.gene001033	PPI
MS.gene001038	MS.gene95802	PPI
MS.gene001038	MS.gene024854	PPI
MS.gene29937	MS.gene001038	PPI
MS.gene024858	MS.gene001038	PPI
MS.gene001038	MS.gene22888	PPI
MS.gene001038	MS.gene04530	PPI
MS.gene024855	MS.gene001038	PPI
MS.gene006418	MS.gene001038	PPI
MS.gene001038	MS.gene024614	PPI
MS.gene001038	MS.gene024612	PPI
MS.gene001038	MS.gene21830	PPI
MS.gene001038	MS.gene34371	PPI
MS.gene001038	MS.gene001034	PPI
MS.gene001038	MS.gene070945	PPI
MS.gene001038	MS.gene071361	PPI
MS.gene001038	MS.gene043813	PPI
MS.gene001038	MS.gene21831	PPI
MS.gene001033	MS.gene001038	PPI
MS.gene001038	MS.gene77154	PPI
MS.gene024854	MS.gene001038	PPI
MS.gene001038	MS.gene006417	PPI
MS.gene001038	MS.gene21827	PPI
MS.gene001038	MS.gene024857	PPI
MS.gene001038	MS.gene070944	PPI
MS.gene001038	MS.gene95801	PPI
MS.gene49468	MS.gene001038	PPI
MS.gene001038	MS.gene49470	PPI
MS.gene001038	MS.gene49468	PPI
MS.gene95802	MS.gene001038	PPI
MS.gene001038	MS.gene024859	PPI
MS.gene34370	MS.gene001038	PPI
MS.gene001038	MS.gene001032	PPI
MS.gene001038	MS.gene74082	PPI
MS.gene49472	MS.gene001038	PPI
MS.gene001038	MS.gene49471	PPI
MS.gene001038	MS.gene006418	PPI
MS.gene77154	MS.gene001038	PPI
MS.gene001038	MS.gene001037	PPI
MS.gene001038	MS.gene21833	PPI
MS.gene21830	MS.gene001038	PPI
MS.gene001038	MS.gene071357	PPI
MS.gene001038	MS.gene050493	PPI
MS.gene001038	MS.gene21828	PPI
MS.gene21833	MS.gene001038	PPI
MS.gene043815	MS.gene001038	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001038.t1	MTR_5g006520	92.632	190	14	0	1	190	1719	1908	1.55e-121	378
MS.gene001038.t1	MTR_5g006450	78.421	190	41	0	1	190	1	190	1.49e-107	306
MS.gene001038.t1	MTR_5g006470	75.789	190	44	1	1	190	1	188	3.98e-103	295
MS.gene001038.t1	MTR_5g006460	70.256	195	51	5	1	190	1	193	7.91e-91	264
MS.gene001038.t1	MTR_5g006510	67.539	191	55	3	1	190	7	191	4.71e-87	254
MS.gene001038.t1	MTR_1g103110	61.458	192	70	2	1	190	1	190	5.91e-83	244
MS.gene001038.t1	MTR_1g103150	59.375	192	74	2	1	190	1	190	6.23e-80	236
MS.gene001038.t1	MTR_7g114410	42.105	171	96	1	17	187	4	171	2.76e-47	153
MS.gene001038.t1	MTR_3g463610	38.953	172	105	0	16	187	2	173	9.44e-45	147
MS.gene001038.t1	MTR_7g114410	41.667	156	88	1	17	172	4	156	3.30e-42	139
MS.gene001038.t1	MTR_7g024760	41.071	168	94	3	20	187	7	169	2.35e-38	130
MS.gene001038.t1	MTR_7g024780	39.080	174	96	3	17	187	4	170	3.37e-37	127
MS.gene001038.t1	MTR_7g024765	39.881	168	96	3	20	187	7	169	1.82e-35	122
MS.gene001038.t1	MTR_1g103320	55.556	81	34	1	51	131	11	89	6.23e-22	86.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene001038.t1	AT2G32030	51.190	168	75	3	21	187	24	185	2.45e-58	181
MS.gene001038.t1	AT2G32020	46.561	189	84	4	2	187	6	180	3.15e-54	171
MS.gene001038.t1	AT3G22560	40.237	169	96	2	21	187	7	172	3.01e-37	127

Find 38 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCTAAGAATTCCTTCCTTC+TGG	0.242611	5.4:+2237546	None:intergenic
ACAGAACTTGCCTATGTTCT+AGG	0.250597	5.4:-2237719	MS.gene001038:CDS
GTTGATGCCTTGTTCTTTGC+TGG	0.260002	5.4:+2237862	None:intergenic
ATGTTCTAGGATCTAAATAT+TGG	0.302360	5.4:-2237706	MS.gene001038:CDS
CCTTGTTCTTTGCTGGTGTA+TGG	0.318147	5.4:+2237869	None:intergenic
TGTTCTAGGATCTAAATATT+GGG	0.318462	5.4:-2237705	MS.gene001038:CDS
TGCCTTAATGATCGTGCTAT+TGG	0.357294	5.4:-2237794	MS.gene001038:CDS
ATATACCAAACAAATTTCTA+TGG	0.388477	5.4:-2237829	MS.gene001038:CDS
TCCAGAAGGAAGGAATTCTT+AGG	0.397920	5.4:-2237547	MS.gene001038:CDS
GGCCAATAGCACGATCATTA+AGG	0.403869	5.4:+2237792	None:intergenic
TTGCACCATAGAAATTTGTT+TGG	0.404833	5.4:+2237824	None:intergenic
TTGTTGATGTTGAAAATGTT+GGG	0.408191	5.4:-2237598	MS.gene001038:CDS
GAAAAGGTCGGAGTGCGATT+TGG	0.410859	5.4:+2237963	None:intergenic
CCAAACAAATAAATCATAGT+TGG	0.419913	5.4:+2237459	None:intergenic
TTCTGACCTTGATGAGCTCC+TGG	0.433077	5.4:-2237936	MS.gene001038:CDS
TTTGTTGATGTTGAAAATGT+TGG	0.442167	5.4:-2237599	MS.gene001038:CDS
AGTGGCCAAGTTCTGCTCTT+GGG	0.462852	5.4:-2237894	MS.gene001038:CDS
AAGTGGCCAAGTTCTGCTCT+TGG	0.474319	5.4:-2237895	MS.gene001038:CDS
GGATCTAAATATTGGGGTAA+AGG	0.475846	5.4:-2237698	MS.gene001038:CDS
AGGAAGTATTTGTTCATGAA+AGG	0.475944	5.4:-2237527	MS.gene001038:CDS
TTTCATCAGCTCTCCATACC+AGG	0.488476	5.4:+2237918	None:intergenic
CAAAGAGTGTTGGAGAAAGC+TGG	0.497277	5.4:-2237572	MS.gene001038:CDS
TCAAGGTCAGAAAGATGAAA+AGG	0.513299	5.4:+2237947	None:intergenic
ATATTTAGATCCTAGAACAT+AGG	0.519203	5.4:+2237709	None:intergenic
ACCTTGATGAGCTCCTGGTA+TGG	0.539184	5.4:-2237931	MS.gene001038:CDS
GATCACCTCATGATAAAAGC+AGG	0.555612	5.4:-2237751	MS.gene001038:CDS
TCCATACCAGGAGCTCATCA+AGG	0.558283	5.4:+2237930	None:intergenic
TGTTGGGTCTCAAAGAGTGT+TGG	0.566749	5.4:-2237582	MS.gene001038:CDS
TGGTTCCCAAGAGCAGAACT+TGG	0.574822	5.4:+2237889	None:intergenic
GTTCTAGGATCTAAATATTG+GGG	0.601221	5.4:-2237704	MS.gene001038:CDS
CCATACACCAGCAAAGAACA+AGG	0.601334	5.4:-2237869	MS.gene001038:CDS
GGAGAAAGCTGGTTTCCAGA+AGG	0.617484	5.4:-2237561	MS.gene001038:CDS
AACTTGTTTCACAACATACG+TGG	0.623050	5.4:+2237670	None:intergenic
AAAGCTGGTTTCCAGAAGGA+AGG	0.623258	5.4:-2237557	MS.gene001038:CDS
GGTCAGAAAGATGAAAAGGT+CGG	0.638104	5.4:+2237951	None:intergenic
TGTTGTGAAACAAGTTGTCA+AGG	0.644645	5.4:-2237663	MS.gene001038:CDS
TTTATTTCTTCCAAACCAGG+TGG	0.661410	5.4:-2238010	MS.gene001038:CDS
ATCTTGATTTCAGAGAAACA+CGG	0.706701	5.4:+2237771	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATACCAAACAAATTTCTA+TGG	-	chr5.4:2237665-2237684	MS.gene001038:CDS	20.0%
!	ATATTTAGATCCTAGAACAT+AGG	+	chr5.4:2237788-2237807	None:intergenic	25.0%
!	TTGTTGATGTTGAAAATGTT+GGG	-	chr5.4:2237896-2237915	MS.gene001038:CDS	25.0%
!	TTTGTTGATGTTGAAAATGT+TGG	-	chr5.4:2237895-2237914	MS.gene001038:CDS	25.0%
!!!	ATGTTCTAGGATCTAAATAT+TGG	-	chr5.4:2237788-2237807	MS.gene001038:CDS	25.0%
!!!	TGTTCTAGGATCTAAATATT+GGG	-	chr5.4:2237789-2237808	MS.gene001038:CDS	25.0%
	ATCTTGATTTCAGAGAAACA+CGG	+	chr5.4:2237726-2237745	None:intergenic	30.0%
!	TTGCACCATAGAAATTTGTT+TGG	+	chr5.4:2237673-2237692	None:intergenic	30.0%
!!	ACTTTTATTTCTTCCAAACC+AGG	-	chr5.4:2237481-2237500	MS.gene001038:CDS	30.0%
!!	AGGAAGTATTTGTTCATGAA+AGG	-	chr5.4:2237967-2237986	MS.gene001038:CDS	30.0%
!!	GTTCTAGGATCTAAATATTG+GGG	-	chr5.4:2237790-2237809	MS.gene001038:CDS	30.0%
	GGATCTAAATATTGGGGTAA+AGG	-	chr5.4:2237796-2237815	MS.gene001038:CDS	35.0%
	TCAAGGTCAGAAAGATGAAA+AGG	+	chr5.4:2237550-2237569	None:intergenic	35.0%
	TGTTGTGAAACAAGTTGTCA+AGG	-	chr5.4:2237831-2237850	MS.gene001038:CDS	35.0%
!	AACTTGTTTCACAACATACG+TGG	+	chr5.4:2237827-2237846	None:intergenic	35.0%
!	TTTATTTCTTCCAAACCAGG+TGG	-	chr5.4:2237484-2237503	MS.gene001038:CDS	35.0%
!!	TTGTTCCTGCTTTTATCATG+AGG	+	chr5.4:2237751-2237770	None:intergenic	35.0%
	ACAGAACTTGCCTATGTTCT+AGG	-	chr5.4:2237775-2237794	MS.gene001038:CDS	40.0%
	ATGGAGAGCTGATGAAAAAG+TGG	-	chr5.4:2237582-2237601	MS.gene001038:CDS	40.0%
	GATCACCTCATGATAAAAGC+AGG	-	chr5.4:2237743-2237762	MS.gene001038:CDS	40.0%
	GGTCAGAAAGATGAAAAGGT+CGG	+	chr5.4:2237546-2237565	None:intergenic	40.0%
	TCCAGAAGGAAGGAATTCTT+AGG	-	chr5.4:2237947-2237966	MS.gene001038:CDS	40.0%
	TCCTAAGAATTCCTTCCTTC+TGG	+	chr5.4:2237951-2237970	None:intergenic	40.0%
	TGCCTTAATGATCGTGCTAT+TGG	-	chr5.4:2237700-2237719	MS.gene001038:CDS	40.0%
	CCATACACCAGCAAAGAACA+AGG	-	chr5.4:2237625-2237644	MS.gene001038:CDS	45.0%
	CCTTGTTCTTTGCTGGTGTA+TGG	+	chr5.4:2237628-2237647	None:intergenic	45.0%
	GGCCAATAGCACGATCATTA+AGG	+	chr5.4:2237705-2237724	None:intergenic	45.0%
	TTTCATCAGCTCTCCATACC+AGG	+	chr5.4:2237579-2237598	None:intergenic	45.0%
!	AAAGCTGGTTTCCAGAAGGA+AGG	-	chr5.4:2237937-2237956	MS.gene001038:CDS	45.0%
!	ATGCTCTTTTCATTGCCACC+TGG	+	chr5.4:2237502-2237521	None:intergenic	45.0%
!	CTTTTCATTGCCACCTGGTT+TGG	+	chr5.4:2237497-2237516	None:intergenic	45.0%
!	GTTGATGCCTTGTTCTTTGC+TGG	+	chr5.4:2237635-2237654	None:intergenic	45.0%
!	TGTTGGGTCTCAAAGAGTGT+TGG	-	chr5.4:2237912-2237931	MS.gene001038:CDS	45.0%
!!	CAAAGAGTGTTGGAGAAAGC+TGG	-	chr5.4:2237922-2237941	MS.gene001038:CDS	45.0%
	AAGTGGCCAAGTTCTGCTCT+TGG	-	chr5.4:2237599-2237618	MS.gene001038:CDS	50.0%
	ACCTTGATGAGCTCCTGGTA+TGG	-	chr5.4:2237563-2237582	MS.gene001038:CDS	50.0%
	AGTGGCCAAGTTCTGCTCTT+GGG	-	chr5.4:2237600-2237619	MS.gene001038:CDS	50.0%
	GAAAAGGTCGGAGTGCGATT+TGG	+	chr5.4:2237534-2237553	None:intergenic	50.0%
	TCCATACCAGGAGCTCATCA+AGG	+	chr5.4:2237567-2237586	None:intergenic	50.0%
	TGGTTCCCAAGAGCAGAACT+TGG	+	chr5.4:2237608-2237627	None:intergenic	50.0%
	TTCTGACCTTGATGAGCTCC+TGG	-	chr5.4:2237558-2237577	MS.gene001038:CDS	50.0%
!	GGAGAAAGCTGGTTTCCAGA+AGG	-	chr5.4:2237933-2237952	MS.gene001038:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	2237472	2238044	2237472	ID=MS.gene001038
chr5.4	mRNA	2237472	2238044	2237472	ID=MS.gene001038.t1;Parent=MS.gene001038
chr5.4	exon	2237472	2238044	2237472	ID=MS.gene001038.t1.exon1;Parent=MS.gene001038.t1
chr5.4	CDS	2237472	2238044	2237472	ID=cds.MS.gene001038.t1;Parent=MS.gene001038.t1

Gene Sequence

>MS.gene001038

ATGGAGGAAACTTTTATTTCTTCCAAACCAGGTGGCAATGAAAAGAGCATTGATTTGAACCAAATCGCACTCCGACCTTTTCATCTTTCTGACCTTGATGAGCTCCTGGTATGGAGAGCTGATGAAAAAGTGGCCAAGTTCTGCTCTTGGGAACCATACACCAGCAAAGAACAAGGCATCAACTTCATTGAAAATATACCAAACAAATTTCTATGGTGCAAAGCAATATGCCTTAATGATCGTGCTATTGGCCGTGTTTCTCTGAAATCAAGATCACCTCATGATAAAAGCAGGAACAAAACTACAGAACTTGCCTATGTTCTAGGATCTAAATATTGGGGTAAAGGAATTGCCACGTATGTTGTGAAACAAGTTGTCAAGGTTGCATTCAGTGAGTTGTCACACTTAGAAAGAGTTGAAGCTTTTGTTGATGTTGAAAATGTTGGGTCTCAAAGAGTGTTGGAGAAAGCTGGTTTCCAGAAGGAAGGAATTCTTAGGAAGTATTTGTTCATGAAAGGAAAAAGTAGAGATATGATCATATTCAGTGTTCTTTCTAATGATCTCCAACTATGA

Protein sequence

>MS.gene001038.t1

MEETFISSKPGGNEKSIDLNQIALRPFHLSDLDELLVWRADEKVAKFCSWEPYTSKEQGINFIENIPNKFLWCKAICLNDRAIGRVSLKSRSPHDKSRNKTTELAYVLGSKYWGKGIATYVVKQVVKVAFSELSHLERVEAFVDVENVGSQRVLEKAGFQKEGILRKYLFMKGKSRDMIIFSVLSNDLQL