AlfalfaGEDB | MS.gene070945

M. sativa cultivar XinJiangDaYe / MS.gene070945

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070945.t1	XP_013469780.1	73.6	182	9	2	2	144	1	182	5.20E-62	247.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070945.t1	P05332	34.2	149	82	4	16	150	30	176	6.1e-11	68.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070945.t1	A0A072VQW9	73.6	182	9	2	2	144	1	182	3.7e-62	247.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene21833	MS.gene070945	PPI
MS.gene043815	MS.gene070945	PPI
MS.gene95802	MS.gene070945	PPI
MS.gene070945	MS.gene22888	PPI
MS.gene070945	MS.gene071361	PPI
MS.gene49472	MS.gene070945	PPI
MS.gene024858	MS.gene070945	PPI
MS.gene070945	MS.gene043813	PPI
MS.gene024859	MS.gene070945	PPI
MS.gene070945	MS.gene21831	PPI
MS.gene024855	MS.gene070945	PPI
MS.gene070945	MS.gene024612	PPI
MS.gene070945	MS.gene34371	PPI
MS.gene001038	MS.gene070945	PPI
MS.gene070945	MS.gene006417	PPI
MS.gene21830	MS.gene070945	PPI
MS.gene070945	MS.gene21827	PPI
MS.gene070945	MS.gene070944	PPI
MS.gene29937	MS.gene070945	PPI
MS.gene006418	MS.gene070945	PPI
MS.gene070945	MS.gene49470	PPI
MS.gene024854	MS.gene070945	PPI
MS.gene070945	MS.gene74082	PPI
MS.gene070945	MS.gene49471	PPI
MS.gene070945	MS.gene024857	PPI
MS.gene070945	MS.gene95801	PPI
MS.gene070945	MS.gene001037	PPI
MS.gene001033	MS.gene070945	PPI
MS.gene070945	MS.gene071357	PPI
MS.gene070945	MS.gene21828	PPI
MS.gene49468	MS.gene070945	PPI
MS.gene070945	MS.gene024859	PPI
MS.gene070945	MS.gene001032	PPI
MS.gene34370	MS.gene070945	PPI
MS.gene070945	MS.gene04530	PPI
MS.gene070945	MS.gene024614	PPI
MS.gene77154	MS.gene070945	PPI
MS.gene070945	MS.gene050493	PPI
MS.gene070945	MS.gene001034	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070945.t1	MTR_1g103110	73.626	182	9	2	2	144	1	182	5.87e-87	253
MS.gene070945.t1	MTR_1g103150	67.582	182	20	2	2	144	1	182	2.33e-81	239
MS.gene070945.t1	MTR_5g006470	64.748	139	40	1	15	144	42	180	1.05e-60	186
MS.gene070945.t1	MTR_5g006450	63.121	141	41	2	15	144	42	182	4.58e-58	180
MS.gene070945.t1	MTR_5g006460	58.621	145	43	5	15	144	43	185	8.56e-48	154
MS.gene070945.t1	MTR_5g006510	56.338	142	48	4	15	144	44	183	9.49e-48	154
MS.gene070945.t1	MTR_5g006520	60.284	141	45	2	15	144	1760	1900	1.71e-47	165
MS.gene070945.t1	MTR_1g103320	56.311	103	35	2	24	116	11	113	1.87e-29	104
MS.gene070945.t1	MTR_7g114410	41.259	143	70	3	13	144	27	166	2.09e-29	106
MS.gene070945.t1	MTR_3g463610	38.356	146	73	3	13	144	26	168	5.77e-29	106
MS.gene070945.t1	MTR_7g114410	41.985	131	62	3	15	134	29	156	2.11e-26	98.6
MS.gene070945.t1	MTR_7g024760	36.879	141	77	4	13	144	27	164	2.58e-21	85.9
MS.gene070945.t1	MTR_7g024780	37.692	130	70	3	24	144	38	165	2.81e-21	85.5
MS.gene070945.t1	MTR_7g024765	36.170	141	78	4	13	144	27	164	2.00e-20	83.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070945.t1	AT2G32030	48.551	138	57	4	17	144	47	180	1.38e-39	133
MS.gene070945.t1	AT2G32020	50.000	138	55	4	17	144	42	175	1.55e-39	132
MS.gene070945.t1	AT3G22560	40.411	146	71	4	15	147	28	170	6.87e-25	95.1

Find 30 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTTCTTTAGCATCTGATT+TGG	0.255230	1.4:+79760585	None:intergenic
CCGTCTTCTTTGTTTGTATA+AGG	0.260514	1.4:+79760447	None:intergenic
TGCTATGAGCTCCAAGTATT+GGG	0.261207	1.4:-79760289	MS.gene070945:CDS
GAGATTTGCTTGCTATGTTT+TGG	0.329625	1.4:+79760412	None:intergenic
TTTAGCATCTGATTTGGAAT+AGG	0.367598	1.4:+79760591	None:intergenic
AATACCTTTGCCCCAATACT+TGG	0.403153	1.4:+79760278	None:intergenic
TGCCTTCGCGATCGAGCAAT+CGG	0.411073	1.4:-79760372	MS.gene070945:CDS
CAGAGGGTGTTGGAGAAGGC+TGG	0.411839	1.4:-79760102	MS.gene070945:CDS
ACATAGCAAGCAAATCTCTA+TGG	0.412043	1.4:-79760407	MS.gene070945:CDS
AGGAACAAATCTGCTGAACT+TGG	0.442081	1.4:-79760315	MS.gene070945:CDS
ATGCTATGAGCTCCAAGTAT+TGG	0.448265	1.4:-79760290	MS.gene070945:CDS
CTTGAGCTTCAAGCCGCTCC+AGG	0.458231	1.4:+79760148	None:intergenic
TTCTCAGAGGGTGTTGGAGA+AGG	0.470170	1.4:-79760106	MS.gene070945:CDS
CAGAAAATATCTGTTCATCA+AGG	0.472630	1.4:-79760058	MS.gene070945:CDS
GCTTCAAGCCGCTCCAGGTA+TGG	0.487071	1.4:+79760153	None:intergenic
AGGTTCCCAAGTGCAATACT+TGG	0.527166	1.4:+79760467	None:intergenic
AGCTCCAAGTATTGGGGCAA+AGG	0.537498	1.4:-79760282	MS.gene070945:intron
TAGAAAATGTGGCTTCTCAG+AGG	0.540854	1.4:-79760119	MS.gene070945:CDS
ATTTAAAGAGTTTCCATACC+TGG	0.542410	1.4:-79760166	MS.gene070945:CDS
GCCTTCGCGATCGAGCAATC+GGG	0.544699	1.4:-79760371	MS.gene070945:CDS
TGTGGCTTCTCAGAGGGTGT+TGG	0.549890	1.4:-79760112	MS.gene070945:CDS
AGAAAATATCTGTTCATCAA+GGG	0.554840	1.4:-79760057	MS.gene070945:CDS
TCAAGTTGATGTAGAAAATG+TGG	0.572167	1.4:-79760130	MS.gene070945:CDS
AAGAGTTTCCATACCTGGAG+CGG	0.585589	1.4:-79760161	MS.gene070945:CDS
AAGTGGCCAAGTATTGCACT+TGG	0.601796	1.4:-79760473	MS.gene070945:CDS
AGAAAATGTGGCTTCTCAGA+GGG	0.613906	1.4:-79760118	MS.gene070945:CDS
CCTTATACAAACAAAGAAGA+CGG	0.623002	1.4:-79760447	MS.gene070945:CDS
ACCCGATTGCTCGATCGCGA+AGG	0.639229	1.4:+79760370	None:intergenic
GCTATGAGCTCCAAGTATTG+GGG	0.656803	1.4:-79760288	MS.gene070945:CDS
AGTGGCCAAGTATTGCACTT+GGG	0.670287	1.4:-79760472	MS.gene070945:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTAGATCAAATATATCAG+TGG	+	chr1.4:79760598-79760617	None:intergenic	20.0%
!	AGAAAATATCTGTTCATCAA+GGG	-	chr1.4:79760501-79760520	MS.gene070945:intron	25.0%
!!!	TATCGATTTCTTTTTGTGTT+CGG	-	chr1.4:79760212-79760231	MS.gene070945:intron	25.0%
	ATTTAAAGAGTTTCCATACC+TGG	-	chr1.4:79760392-79760411	MS.gene070945:CDS	30.0%
	CAAGATCTGAAAGACTAATT+GGG	+	chr1.4:79760035-79760054	None:intergenic	30.0%
	CAGAAAATATCTGTTCATCA+AGG	-	chr1.4:79760500-79760519	MS.gene070945:intron	30.0%
	CCTTATACAAACAAAGAAGA+CGG	-	chr1.4:79760111-79760130	MS.gene070945:CDS	30.0%
	TCAAGATCTGAAAGACTAAT+TGG	+	chr1.4:79760036-79760055	None:intergenic	30.0%
!!	TCAAGTTGATGTAGAAAATG+TGG	-	chr1.4:79760428-79760447	MS.gene070945:CDS	30.0%
!!	TTTAGCATCTGATTTGGAAT+AGG	+	chr1.4:79759970-79759989	None:intergenic	30.0%
!!!	TTCAGATCTTGATGATGTTT+TGG	-	chr1.4:79760044-79760063	MS.gene070945:CDS	30.0%
	AATTGGGTCGAAGAGATATT+TGG	+	chr1.4:79760019-79760038	None:intergenic	35.0%
	ACATAGCAAGCAAATCTCTA+TGG	-	chr1.4:79760151-79760170	MS.gene070945:CDS	35.0%
	CAAGGGAAAAAGTAGAGATA+TGG	-	chr1.4:79760518-79760537	MS.gene070945:intron	35.0%
	CTCTTCTTTAGCATCTGATT+TGG	+	chr1.4:79759976-79759995	None:intergenic	35.0%
!	CCGTCTTCTTTGTTTGTATA+AGG	+	chr1.4:79760114-79760133	None:intergenic	35.0%
!!	ACCACTTTTGACAAAAAGTG+TGG	+	chr1.4:79760363-79760382	None:intergenic	35.0%
!!!	ATCTTGATGATGTTTTGGTG+TGG	-	chr1.4:79760049-79760068	MS.gene070945:CDS	35.0%
!!!	GAGATTTGCTTGCTATGTTT+TGG	+	chr1.4:79760149-79760168	None:intergenic	35.0%
	AATACCTTTGCCCCAATACT+TGG	+	chr1.4:79760283-79760302	None:intergenic	40.0%
	AGAAAATGTGGCTTCTCAGA+GGG	-	chr1.4:79760440-79760459	MS.gene070945:CDS	40.0%
	AGGAACAAATCTGCTGAACT+TGG	-	chr1.4:79760243-79760262	MS.gene070945:intron	40.0%
	ATGCTATGAGCTCCAAGTAT+TGG	-	chr1.4:79760268-79760287	MS.gene070945:intron	40.0%
	GTGGACAAGTGATGAAAAAG+TGG	-	chr1.4:79760068-79760087	MS.gene070945:CDS	40.0%
	TAGAAAATGTGGCTTCTCAG+AGG	-	chr1.4:79760439-79760458	MS.gene070945:CDS	40.0%
	TGCTATGAGCTCCAAGTATT+GGG	-	chr1.4:79760269-79760288	MS.gene070945:intron	40.0%
!	TTTTGTGTTCGGATAGATGC+AGG	-	chr1.4:79760223-79760242	MS.gene070945:intron	40.0%
!!	GCCACACTTTTTGTCAAAAG+TGG	-	chr1.4:79760359-79760378	MS.gene070945:CDS	40.0%
	AAGAGTTTCCATACCTGGAG+CGG	-	chr1.4:79760397-79760416	MS.gene070945:CDS	45.0%
	AGGTTCCCAAGTGCAATACT+TGG	+	chr1.4:79760094-79760113	None:intergenic	45.0%
	TATTGCCACACTGCTGAACT+TGG	-	chr1.4:79760299-79760318	MS.gene070945:CDS	45.0%
!	AAGTGGCCAAGTATTGCACT+TGG	-	chr1.4:79760085-79760104	MS.gene070945:CDS	45.0%
!	AGTGGCCAAGTATTGCACTT+GGG	-	chr1.4:79760086-79760105	MS.gene070945:CDS	45.0%
!	GCTATGAGCTCCAAGTATTG+GGG	-	chr1.4:79760270-79760289	MS.gene070945:intron	45.0%
!!!	AAGGCTGGTTTTCAAAGGGA+GGG	-	chr1.4:79760471-79760490	MS.gene070945:CDS	45.0%
!!!	GGAGAAGGCTGGTTTTCAAA+GGG	-	chr1.4:79760467-79760486	MS.gene070945:CDS	45.0%
!!!	TGGAGAAGGCTGGTTTTCAA+AGG	-	chr1.4:79760466-79760485	MS.gene070945:CDS	45.0%
	CATAGCCAAGTTCAGCAGTG+TGG	+	chr1.4:79760307-79760326	None:intergenic	50.0%
!!	AGCTCCAAGTATTGGGGCAA+AGG	-	chr1.4:79760276-79760295	MS.gene070945:intron	50.0%
!!	TTCTCAGAGGGTGTTGGAGA+AGG	-	chr1.4:79760452-79760471	MS.gene070945:CDS	50.0%
!!!	GAAGGCTGGTTTTCAAAGGG+AGG	-	chr1.4:79760470-79760489	MS.gene070945:CDS	50.0%
	TGCCTTCGCGATCGAGCAAT+CGG	-	chr1.4:79760186-79760205	MS.gene070945:intron	55.0%
!	TGTGGCTTCTCAGAGGGTGT+TGG	-	chr1.4:79760446-79760465	MS.gene070945:CDS	55.0%
	ACCCGATTGCTCGATCGCGA+AGG	+	chr1.4:79760191-79760210	None:intergenic	60.0%
	CTTGAGCTTCAAGCCGCTCC+AGG	+	chr1.4:79760413-79760432	None:intergenic	60.0%
	GCCTTCGCGATCGAGCAATC+GGG	-	chr1.4:79760187-79760206	MS.gene070945:intron	60.0%
	GCTTCAAGCCGCTCCAGGTA+TGG	+	chr1.4:79760408-79760427	None:intergenic	60.0%
!!	CAGAGGGTGTTGGAGAAGGC+TGG	-	chr1.4:79760456-79760475	MS.gene070945:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	79759948	79760632	79759948	ID=MS.gene070945
chr1.4	mRNA	79759948	79760632	79759948	ID=MS.gene070945.t1;Parent=MS.gene070945
chr1.4	exon	79760584	79760632	79760584	ID=MS.gene070945.t1.exon1;Parent=MS.gene070945.t1
chr1.4	CDS	79760584	79760632	79760584	ID=cds.MS.gene070945.t1;Parent=MS.gene070945.t1
chr1.4	exon	79760283	79760492	79760283	ID=MS.gene070945.t1.exon2;Parent=MS.gene070945.t1
chr1.4	CDS	79760283	79760492	79760283	ID=cds.MS.gene070945.t1;Parent=MS.gene070945.t1
chr1.4	exon	79759948	79760201	79759948	ID=MS.gene070945.t1.exon3;Parent=MS.gene070945.t1
chr1.4	CDS	79759948	79760201	79759948	ID=cds.MS.gene070945.t1;Parent=MS.gene070945.t1

Gene Sequence

>MS.gene070945

ATGATGGAAAAAACTGCTACCTATTCCAAATCAGATGCTAAAGAAGAGTGTTCTGTTGTTGACTTGAGCCAAATATCTCTTCGACCCAATTAGTCTTTCAGATCTTGATGATGTTTTGGTGTGGACAAGTGATGAAAAAGTGGCCAAGTATTGCACTTGGGAACCTTATACAAACAAAGAAGACGGCATTGACTTCATCCAAAACATAGCAAGCAAATCTCTATGGTTCAGAGCAATTTGCCTTCGCGATCGAGCAATCGGGTGTATCGATTTCTTTTTGTGTTCGGATAGATGCAGGAACAAATCTGCTGAACTTGGCTATGCTATGAGCTCCAAGTATTGGGGCAAAGGTATTGCCACACTGCTGAACTTGGCTATGCTATGAGCTCCAAGTATTGGGGCAAAGGTATTGCCACACTTTTTGTCAAAAGTGGTTGAAGCTGCATTTAAAGAGTTTCCATACCTGGAGCGGCTTGAAGCTCAAGTTGATGTAGAAAATGTGGCTTCTCAGAGGGTGTTGGAGAAGGCTGGTTTTCAAAGGGAGGGTGTTCTCAGAAAATATCTGTTCATCAAGGGAAAAAGTAGAGATATGGTTATGTTCTCTCAAGTGAAGTTCAAATTTGATCTGTTTGTTTTCAAGTATGTGACCACTGATATATTTGATCTAAAACATCTTCAGTATTAG

Protein sequence

>MS.gene070945.t1

MMEKTATYSKSDAKEELAKYCTWEPYTNKEDGIDFIQNIASKSLWFRAICLRDRAIGCIDFFLCSDRCRNKSAELGYAMSSKYWGKVVEAAFKEFPYLERLEAQVDVENVASQRVLEKAGFQREGVLRKYLFIKGKSRDMVMFSQVKFKFDLFVFKYVTTDIFDLKHLQY