AlfalfaGEDB | MS.gene024614

M. sativa cultivar XinJiangDaYe / MS.gene024614

KEGG: -
GO: -
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024614.t1	XP_003626377.2	97.2	176	5	0	1	176	1	176	5.00E-95	357.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024614.t1	O34569	29.7	175	115	4	4	172	5	177	8.3e-14	78.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024614.t1	G7L0S4	97.2	176	5	0	1	176	1	176	3.6e-95	357.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene024614.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene024855	MS.gene024614	PPI
MS.gene77154	MS.gene024614	PPI
MS.gene024614	MS.gene071357	PPI
MS.gene043813	MS.gene024614	PPI
MS.gene34371	MS.gene024614	PPI
MS.gene001038	MS.gene024614	PPI
MS.gene49470	MS.gene024614	PPI
MS.gene21830	MS.gene024614	PPI
MS.gene21833	MS.gene024614	PPI
MS.gene82121	MS.gene024614	PPI
MS.gene001034	MS.gene024614	PPI
MS.gene21831	MS.gene024614	PPI
MS.gene95801	MS.gene024614	PPI
MS.gene024858	MS.gene024614	PPI
MS.gene024859	MS.gene024614	PPI
MS.gene006418	MS.gene024614	PPI
MS.gene83474	MS.gene024614	PPI
MS.gene024854	MS.gene024614	PPI
MS.gene024614	MS.gene72809	PPI
MS.gene028111	MS.gene024614	PPI
MS.gene001037	MS.gene024614	PPI
MS.gene006417	MS.gene024614	PPI
MS.gene001033	MS.gene024614	PPI
MS.gene21827	MS.gene024614	PPI
MS.gene49472	MS.gene024614	PPI
MS.gene49468	MS.gene024614	PPI
MS.gene024614	MS.gene024857	PPI
MS.gene024614	MS.gene070944	PPI
MS.gene49471	MS.gene024614	PPI
MS.gene071361	MS.gene024614	PPI
MS.gene070945	MS.gene024614	PPI
MS.gene024614	MS.gene001032	PPI
MS.gene043815	MS.gene024614	PPI
MS.gene21828	MS.gene024614	PPI
MS.gene95802	MS.gene024614	PPI
MS.gene34370	MS.gene024614	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024614.t1	MTR_7g114410	97.159	176	5	0	1	176	1	176	1.28e-126	353
MS.gene024614.t1	MTR_7g114410	96.795	156	5	0	1	156	1	156	2.51e-111	314
MS.gene024614.t1	MTR_3g463610	62.573	171	61	1	4	171	3	173	6.65e-80	237
MS.gene024614.t1	MTR_7g024780	58.523	176	72	1	1	176	1	175	4.49e-71	213
MS.gene024614.t1	MTR_7g024765	61.905	168	62	2	7	174	7	172	2.62e-67	203
MS.gene024614.t1	MTR_7g024760	59.195	174	69	2	1	174	1	172	5.04e-67	202
MS.gene024614.t1	MTR_1g103150	46.784	171	86	2	4	171	19	187	6.40e-48	154
MS.gene024614.t1	MTR_1g103110	45.614	171	88	2	4	171	19	187	7.23e-47	152
MS.gene024614.t1	MTR_5g006450	42.442	172	96	1	3	171	16	187	7.72e-47	152
MS.gene024614.t1	MTR_5g006520	44.444	171	92	1	4	171	1735	1905	2.64e-45	159
MS.gene024614.t1	MTR_5g006470	43.787	169	94	1	4	171	17	185	1.20e-44	146
MS.gene024614.t1	MTR_5g006510	42.442	172	93	3	4	171	19	188	8.21e-42	139
MS.gene024614.t1	MTR_5g006460	43.429	175	90	5	4	171	18	190	8.33e-42	139

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024614.t1	AT3G22560	54.819	166	73	1	8	171	7	172	6.89e-63	192
MS.gene024614.t1	AT2G32020	48.276	174	86	2	1	173	12	182	2.75e-57	178
MS.gene024614.t1	AT2G32030	47.305	167	84	2	8	173	24	187	4.05e-57	178

Find 41 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTGTTGCTTCTAACTATT+GGG	0.152318	7.3:-2484609	MS.gene024614:CDS
TTCTGATTTGTTAAGGTTGC+AGG	0.201561	7.3:-2484528	MS.gene024614:CDS
ATGCTGTTGCTTCTAACTAT+TGG	0.211993	7.3:-2484610	MS.gene024614:CDS
CAAGGCTTCTCAAAGAGTTT+TGG	0.245031	7.3:-2484486	MS.gene024614:CDS
AGACATCTTTGATGAAAGTT+AGG	0.305845	7.3:+2484741	None:intergenic
CAAAGGTACCATCATGGATT+TGG	0.335385	7.3:-2484414	MS.gene024614:CDS
AATAGATCATAAAGAGTAAC+AGG	0.344936	7.3:+2484337	None:intergenic
CGTTGACGATTTCATGTTAT+GGG	0.361603	7.3:-2484825	MS.gene024614:CDS
TTCTAAGAACACCCTCTCTA+AGG	0.383141	7.3:+2484450	None:intergenic
ACGTTGACGATTTCATGTTA+TGG	0.384226	7.3:-2484826	MS.gene024614:CDS
TCAACGTCTGTTAGCTTGAA+TGG	0.418956	7.3:+2484842	None:intergenic
GACGATTTCATGTTATGGGC+AGG	0.454598	7.3:-2484821	MS.gene024614:CDS
GACATCTTTGATGAAAGTTA+GGG	0.457791	7.3:+2484742	None:intergenic
CAGGAGACAAAATAATCAAC+AGG	0.459432	7.3:+2484356	None:intergenic
TGGCAAAGGTGGATCTATCT+AGG	0.472926	7.3:-2484877	MS.gene024614:CDS
CGATTTCATGTTATGGGCAG+GGG	0.502678	7.3:-2484819	MS.gene024614:CDS
GCTTCTAACTATTGGGGTCA+AGG	0.506483	7.3:-2484602	MS.gene024614:CDS
ATCGAATGACGCCAAGGGTG+AGG	0.527528	7.3:+2484713	None:intergenic
TGTCTCGACGACCGTTCCAT+CGG	0.528351	7.3:-2484689	MS.gene024614:CDS
GCTGTTGCTTCTAACTATTG+GGG	0.550437	7.3:-2484608	MS.gene024614:CDS
AGAAAGTATACTTATATCAA+AGG	0.562044	7.3:-2484431	MS.gene024614:CDS
ATCCTTGGTCAGCTGATGAT+AGG	0.563579	7.3:-2484652	MS.gene024614:CDS
CTTCTAACTATTGGGGTCAA+GGG	0.563961	7.3:-2484601	MS.gene024614:CDS
TTTGACCAACATGGCAAAGG+TGG	0.564105	7.3:-2484888	None:intergenic
GATGGAAAACTTGCAATTCG+AGG	0.572925	7.3:-2484772	MS.gene024614:CDS
ACGATTTCATGTTATGGGCA+GGG	0.580454	7.3:-2484820	MS.gene024614:CDS
GATAGGTGCAAAGCTGACAT+AGG	0.586688	7.3:-2484635	MS.gene024614:CDS
ATGGAAAACTTGCAATTCGA+GGG	0.591968	7.3:-2484771	MS.gene024614:CDS
ATGTCTGCATACCTCACCCT+TGG	0.593939	7.3:-2484724	MS.gene024614:CDS
TTATATCAAAGGTACCATCA+TGG	0.596125	7.3:-2484420	MS.gene024614:CDS
AGACAGATCGAATGACGCCA+AGG	0.596639	7.3:+2484707	None:intergenic
TCATTTGACCAACATGGCAA+AGG	0.601751	7.3:-2484891	None:intergenic
AAAGGCTGGGTTCCTTAGAG+AGG	0.612674	7.3:-2484462	MS.gene024614:CDS
ATACACAGAAACAAAACCGA+TGG	0.615405	7.3:+2484673	None:intergenic
AAAGACAGCCAAATCCATGA+TGG	0.623533	7.3:+2484406	None:intergenic
GACAGATCGAATGACGCCAA+GGG	0.628805	7.3:+2484708	None:intergenic
CAGAAACAAAACCGATGGAA+CGG	0.641063	7.3:+2484678	None:intergenic
CACCTATCATCAGCTGACCA+AGG	0.642563	7.3:+2484650	None:intergenic
AAGGCTGGGTTCCTTAGAGA+GGG	0.646930	7.3:-2484461	MS.gene024614:CDS
GAGACAAAATAATCAACAGG+AGG	0.670315	7.3:+2484359	None:intergenic
ATCTATCTAGGATATCTCTG+AGG	0.735761	7.3:-2484865	MS.gene024614:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGAAAGTATACTTATATCAA+AGG	-	chr7.3:2484790-2484809	MS.gene024614:CDS	20.0%
!!!	ATGATTTTTCTGATTTGTTA+AGG	-	chr7.3:2484686-2484705	MS.gene024614:CDS	20.0%
	AGACATCTTTGATGAAAGTT+AGG	+	chr7.3:2484483-2484502	None:intergenic	30.0%
	GACATCTTTGATGAAAGTTA+GGG	+	chr7.3:2484482-2484501	None:intergenic	30.0%
!	TTTTACTTACGTGGATAACA+AGG	-	chr7.3:2484717-2484736	MS.gene024614:CDS	30.0%
!!	TTATATCAAAGGTACCATCA+TGG	-	chr7.3:2484801-2484820	MS.gene024614:CDS	30.0%
!!!	AGCAATTTTGATTGCTTTTG+TGG	+	chr7.3:2484650-2484669	None:intergenic	30.0%
!!!	TTCTCAAAGAGTTTTGGAAA+AGG	-	chr7.3:2484741-2484760	MS.gene024614:CDS	30.0%
	AAGTAACCAAAAACATCCGA+TGG	-	chr7.3:2484431-2484450	MS.gene024614:CDS	35.0%
	ACGTTGACGATTTCATGTTA+TGG	-	chr7.3:2484395-2484414	MS.gene024614:CDS	35.0%
	ATACACAGAAACAAAACCGA+TGG	+	chr7.3:2484551-2484570	None:intergenic	35.0%
	ATGGAAAACTTGCAATTCGA+GGG	-	chr7.3:2484450-2484469	MS.gene024614:CDS	35.0%
	CAGGAGACAAAATAATCAAC+AGG	+	chr7.3:2484868-2484887	None:intergenic	35.0%
	CGTTGACGATTTCATGTTAT+GGG	-	chr7.3:2484396-2484415	MS.gene024614:CDS	35.0%
	GAGACAAAATAATCAACAGG+AGG	+	chr7.3:2484865-2484884	None:intergenic	35.0%
!	AGTTTTCCATCGGATGTTTT+TGG	+	chr7.3:2484440-2484459	None:intergenic	35.0%
!	ATCTATCTAGGATATCTCTG+AGG	-	chr7.3:2484356-2484375	MS.gene024614:CDS	35.0%
!	ATGCTGTTGCTTCTAACTAT+TGG	-	chr7.3:2484611-2484630	MS.gene024614:CDS	35.0%
!	TCGAATTGCAAGTTTTCCAT+CGG	+	chr7.3:2484450-2484469	None:intergenic	35.0%
!	TGCTGTTGCTTCTAACTATT+GGG	-	chr7.3:2484612-2484631	MS.gene024614:CDS	35.0%
!!	TTCTGATTTGTTAAGGTTGC+AGG	-	chr7.3:2484693-2484712	MS.gene024614:CDS	35.0%
!!!	AAAGAGTTTTGGAAAAGGCT+GGG	-	chr7.3:2484746-2484765	MS.gene024614:CDS	35.0%
!!!	GTTTTGTTTCTGTGTATCCT+TGG	-	chr7.3:2484554-2484573	MS.gene024614:CDS	35.0%
	AAAGACAGCCAAATCCATGA+TGG	+	chr7.3:2484818-2484837	None:intergenic	40.0%
	ACGATTTCATGTTATGGGCA+GGG	-	chr7.3:2484401-2484420	MS.gene024614:CDS	40.0%
	CAGAAACAAAACCGATGGAA+CGG	+	chr7.3:2484546-2484565	None:intergenic	40.0%
	CTTCTAACTATTGGGGTCAA+GGG	-	chr7.3:2484620-2484639	MS.gene024614:CDS	40.0%
	GATGGAAAACTTGCAATTCG+AGG	-	chr7.3:2484449-2484468	MS.gene024614:CDS	40.0%
!	CAAGGCTTCTCAAAGAGTTT+TGG	-	chr7.3:2484735-2484754	MS.gene024614:CDS	40.0%
!	GCTGTTGCTTCTAACTATTG+GGG	-	chr7.3:2484613-2484632	MS.gene024614:CDS	40.0%
!	TCAACGTCTGTTAGCTTGAA+TGG	+	chr7.3:2484382-2484401	None:intergenic	40.0%
!	TTCTAAGAACACCCTCTCTA+AGG	+	chr7.3:2484774-2484793	None:intergenic	40.0%
!!	CAAAGGTACCATCATGGATT+TGG	-	chr7.3:2484807-2484826	MS.gene024614:CDS	40.0%
!!!	CAAAGAGTTTTGGAAAAGGC+TGG	-	chr7.3:2484745-2484764	MS.gene024614:CDS	40.0%
	ATCCTTGGTCAGCTGATGAT+AGG	-	chr7.3:2484569-2484588	MS.gene024614:CDS	45.0%
	CGATTTCATGTTATGGGCAG+GGG	-	chr7.3:2484402-2484421	MS.gene024614:CDS	45.0%
	GACGATTTCATGTTATGGGC+AGG	-	chr7.3:2484400-2484419	MS.gene024614:CDS	45.0%
	GATAGGTGCAAAGCTGACAT+AGG	-	chr7.3:2484586-2484605	MS.gene024614:CDS	45.0%
	GCTTCTAACTATTGGGGTCA+AGG	-	chr7.3:2484619-2484638	MS.gene024614:CDS	45.0%
	TGGCAAAGGTGGATCTATCT+AGG	-	chr7.3:2484344-2484363	MS.gene024614:CDS	45.0%
!	GTTGCAGGCTTTTACTTACG+TGG	-	chr7.3:2484708-2484727	MS.gene024614:CDS	45.0%
	AAAGGCTGGGTTCCTTAGAG+AGG	-	chr7.3:2484759-2484778	MS.gene024614:CDS	50.0%
	AAGGCTGGGTTCCTTAGAGA+GGG	-	chr7.3:2484760-2484779	MS.gene024614:CDS	50.0%
	AGACAGATCGAATGACGCCA+AGG	+	chr7.3:2484517-2484536	None:intergenic	50.0%
	ATGTCTGCATACCTCACCCT+TGG	-	chr7.3:2484497-2484516	MS.gene024614:CDS	50.0%
	CACCTATCATCAGCTGACCA+AGG	+	chr7.3:2484574-2484593	None:intergenic	50.0%
	GACAGATCGAATGACGCCAA+GGG	+	chr7.3:2484516-2484535	None:intergenic	50.0%
	ATCGAATGACGCCAAGGGTG+AGG	+	chr7.3:2484511-2484530	None:intergenic	55.0%
	TGTCTCGACGACCGTTCCAT+CGG	-	chr7.3:2484532-2484551	MS.gene024614:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	2484343	2484900	2484343	ID=MS.gene024614
chr7.3	mRNA	2484343	2484900	2484343	ID=MS.gene024614.t1;Parent=MS.gene024614
chr7.3	exon	2484343	2484900	2484343	ID=MS.gene024614.t1.exon1;Parent=MS.gene024614.t1
chr7.3	CDS	2484343	2484900	2484343	ID=cds.MS.gene024614.t1;Parent=MS.gene024614.t1

Gene Sequence

>MS.gene024614

ATGGCAAAGGTGGATCTATCTAGGATATCTCTGAGGCCATTCAAGCTAACAGACGTTGACGATTTCATGTTATGGGCAGGGGACGATGAAGTAACCAAAAACATCCGATGGAAAACTTGCAATTCGAGGGAAGAAGCCCTAACTTTCATCAAAGATGTCTGCATACCTCACCCTTGGCGTCATTCGATCTGTCTCGACGACCGTTCCATCGGTTTTGTTTCTGTGTATCCTTGGTCAGCTGATGATAGGTGCAAAGCTGACATAGGTTATGCTGTTGCTTCTAACTATTGGGGTCAAGGGATAGCCACAAAAGCAATCAAAATTGCTCTGTCTCAAGTGTTTAATGATTTTTCTGATTTGTTAAGGTTGCAGGCTTTTACTTACGTGGATAACAAGGCTTCTCAAAGAGTTTTGGAAAAGGCTGGGTTCCTTAGAGAGGGTGTTCTTAGAAAGTATACTTATATCAAAGGTACCATCATGGATTTGGCTGTCTTTAGTTTTTTGTCAACAGATGAAATTCCTCCTGTTGATTATTTTGTCTCCTGTTACTCTTTATGA

Protein sequence

>MS.gene024614.t1

MAKVDLSRISLRPFKLTDVDDFMLWAGDDEVTKNIRWKTCNSREEALTFIKDVCIPHPWRHSICLDDRSIGFVSVYPWSADDRCKADIGYAVASNYWGQGIATKAIKIALSQVFNDFSDLLRLQAFTYVDNKASQRVLEKAGFLREGVLRKYTYIKGTIMDLAVFSFLSTDEIPPVDYFVSCYSL