AlfalfaGEDB | MS.gene04530

M. sativa cultivar XinJiangDaYe / MS.gene04530

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04530.t1	KEH34246.1	97.2	178	5	0	1	178	1	178	3.50E-93	350.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04530.t1	P05332	35.9	170	104	3	7	173	8	175	9.1e-18	91.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04530.t1	A0A072UXR7	97.2	178	5	0	1	178	1	178	2.5e-93	350.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene04530.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene043813	MS.gene04530	PPI
MS.gene34371	MS.gene04530	PPI
MS.gene001038	MS.gene04530	PPI
MS.gene21830	MS.gene04530	PPI
MS.gene21833	MS.gene04530	PPI
MS.gene043815	MS.gene04530	PPI
MS.gene001034	MS.gene04530	PPI
MS.gene21831	MS.gene04530	PPI
MS.gene95801	MS.gene04530	PPI
MS.gene024858	MS.gene04530	PPI
MS.gene024859	MS.gene04530	PPI
MS.gene024855	MS.gene04530	PPI
MS.gene006418	MS.gene04530	PPI
MS.gene83474	MS.gene04530	PPI
MS.gene49470	MS.gene04530	PPI
MS.gene82121	MS.gene04530	PPI
MS.gene04530	MS.gene024857	PPI
MS.gene028111	MS.gene04530	PPI
MS.gene001037	MS.gene04530	PPI
MS.gene006417	MS.gene04530	PPI
MS.gene070944	MS.gene04530	PPI
MS.gene001033	MS.gene04530	PPI
MS.gene21827	MS.gene04530	PPI
MS.gene49468	MS.gene04530	PPI
MS.gene04530	MS.gene071357	PPI
MS.gene024854	MS.gene04530	PPI
MS.gene001032	MS.gene04530	PPI
MS.gene49471	MS.gene04530	PPI
MS.gene071361	MS.gene04530	PPI
MS.gene72809	MS.gene04530	PPI
MS.gene070945	MS.gene04530	PPI
MS.gene21828	MS.gene04530	PPI
MS.gene95802	MS.gene04530	PPI
MS.gene34370	MS.gene04530	PPI
MS.gene49472	MS.gene04530	PPI
MS.gene77154	MS.gene04530	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04530.t1	MTR_3g463610	97.191	178	5	0	1	178	1	178	1.40e-127	357
MS.gene04530.t1	MTR_7g114410	62.573	171	61	1	3	173	4	171	4.15e-80	236
MS.gene04530.t1	MTR_7g114410	63.462	156	54	1	3	158	4	156	6.48e-74	219
MS.gene04530.t1	MTR_7g024765	51.190	168	77	2	6	173	7	169	1.78e-54	171
MS.gene04530.t1	MTR_7g024760	51.190	168	77	2	6	173	7	169	1.14e-52	166
MS.gene04530.t1	MTR_7g024780	48.538	171	84	2	3	173	4	170	6.36e-52	164
MS.gene04530.t1	MTR_1g103110	43.750	176	91	2	3	175	19	189	6.26e-48	154
MS.gene04530.t1	MTR_1g103150	43.750	176	91	2	3	175	19	189	1.66e-46	150
MS.gene04530.t1	MTR_5g006450	39.306	173	105	0	3	175	17	189	3.64e-44	145
MS.gene04530.t1	MTR_5g006520	41.860	172	100	0	2	173	1734	1905	5.15e-43	152
MS.gene04530.t1	MTR_5g006510	41.860	172	97	3	3	173	19	188	3.43e-41	137
MS.gene04530.t1	MTR_5g006470	40.230	174	100	2	3	175	17	187	9.15e-39	131
MS.gene04530.t1	MTR_5g006460	41.379	174	98	3	3	173	18	190	2.79e-38	130

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04530.t1	AT2G32030	47.337	169	82	2	7	174	24	186	4.06e-58	180
MS.gene04530.t1	AT3G22560	49.714	175	83	3	1	173	1	172	1.85e-55	173
MS.gene04530.t1	AT2G32020	46.746	169	83	2	7	174	19	181	3.64e-51	162

Find 45 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTTTAGCTGCTAACTATT+GGG	0.244053	3.4:+58973576	MS.gene04530:CDS
ATGCTTTAGCTGCTAACTAT+TGG	0.297415	3.4:+58973575	MS.gene04530:CDS
TTTCAAGGATATCCCTGATT+TGG	0.317746	3.4:+58973645	MS.gene04530:CDS
TGGAAAAGGTTGGGTTCCTT+AGG	0.326822	3.4:+58973719	MS.gene04530:CDS
GCTTGTATCCTAACCAAATC+AGG	0.336296	3.4:-58973658	None:intergenic
CTGAGGGTCTTGGAAAAGGT+TGG	0.345366	3.4:+58973709	MS.gene04530:CDS
AGTTTGATTGTCAATGCATA+TGG	0.363098	3.4:-58973482	None:intergenic
TGAGGGTCTTGGAAAAGGTT+GGG	0.395764	3.4:+58973710	MS.gene04530:CDS
AGGATATCCCTGATTTGGTT+AGG	0.407728	3.4:+58973650	MS.gene04530:CDS
TCAAGCTTCTCTGAGGGTCT+TGG	0.408935	3.4:+58973699	MS.gene04530:CDS
TTCTCTGAGGGTCTTGGAAA+AGG	0.416298	3.4:+58973705	MS.gene04530:CDS
TACAGCAACTTTCACTGCTT+GGG	0.434215	3.4:-58973614	None:intergenic
TTCATATCAATTTCTCCAGC+TGG	0.435051	3.4:+58973511	MS.gene04530:CDS
GGAAAAGGTTGGGTTCCTTA+GGG	0.443455	3.4:+58973720	MS.gene04530:CDS
TCAATTTCTCCAGCTGGATC+TGG	0.461550	3.4:+58973517	MS.gene04530:CDS
GGTGAGGAATGCACACATCT+TGG	0.477512	3.4:-58973447	None:intergenic
ATACAGCAACTTTCACTGCT+TGG	0.488169	3.4:-58973615	None:intergenic
AGAAAGTATTCGTATGTCAA+AGG	0.504196	3.4:+58973754	MS.gene04530:CDS
GATCTGGTGATGATGATGAT+AGG	0.507122	3.4:+58973533	MS.gene04530:CDS
ATGTGTGCATTCCTCACCCC+TGG	0.509851	3.4:+58973452	MS.gene04530:CDS
AATGCATATGGATCGAGTCC+AGG	0.509920	3.4:-58973470	None:intergenic
GAAAGTATTCGTATGTCAAA+GGG	0.510894	3.4:+58973755	MS.gene04530:CDS
GACGATTTCATGTTAGTAGC+AGG	0.511049	3.4:+58973355	MS.gene04530:CDS
AATGAGTGTCCTCTGACAAA+AGG	0.523146	3.4:-58973804	None:intergenic
ATGGATCGAGTCCAGGGGTG+AGG	0.540320	3.4:-58973463	None:intergenic
GCTGCTAACTATTGGGGTCA+AGG	0.542202	3.4:+58973583	MS.gene04530:CDS
TGCATTGACAATCAAACTAT+CGG	0.542819	3.4:+58973487	MS.gene04530:CDS
GCTTTAGCTGCTAACTATTG+GGG	0.545318	3.4:+58973577	MS.gene04530:CDS
GATAGGTGTAGGGCTGAGAT+TGG	0.546473	3.4:+58973550	MS.gene04530:CDS
GTATGTCAAAGGGAGAGTTA+AGG	0.550149	3.4:+58973765	MS.gene04530:CDS
AAAGGTTGGGTTCCTTAGGG+AGG	0.565250	3.4:+58973723	MS.gene04530:CDS
ATGCATATGGATCGAGTCCA+GGG	0.566733	3.4:-58973469	None:intergenic
TCATCATCACCAGATCCAGC+TGG	0.579658	3.4:-58973526	None:intergenic
CTTGTATCCTAACCAAATCA+GGG	0.587926	3.4:-58973657	None:intergenic
TTCTGAGTATACCCTCCCTA+AGG	0.601810	3.4:-58973735	None:intergenic
TGGAGAATCAAGCTTCTCTG+AGG	0.619862	3.4:+58973692	MS.gene04530:CDS
AAGGTTGGGTTCCTTAGGGA+GGG	0.621218	3.4:+58973724	MS.gene04530:CDS
GTGATGATGATGATAGGTGT+AGG	0.623007	3.4:+58973539	MS.gene04530:CDS
GAGTGACAAACTATACTAGA+CGG	0.627131	3.4:+58973386	MS.gene04530:CDS
TGATGATGATGATAGGTGTA+GGG	0.628441	3.4:+58973540	MS.gene04530:CDS
TCGACGTCACTCAATTCGAA+TGG	0.631370	3.4:-58973334	None:intergenic
ACTCAATTCGAATGGCCGCA+AGG	0.642027	3.4:-58973326	None:intergenic
GGAGAATCAAGCTTCTCTGA+GGG	0.652700	3.4:+58973693	MS.gene04530:CDS
TGCATATGGATCGAGTCCAG+GGG	0.689645	3.4:-58973468	None:intergenic
ACTTGTCAAGAATCACCTTG+CGG	0.733741	3.4:+58973311	MS.gene04530:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGTTTGATTGTCAATGCATA+TGG	-	chr3.4:58973485-58973504	None:intergenic	30.0%
!	TGCATTGACAATCAAACTAT+CGG	+	chr3.4:58973487-58973506	MS.gene04530:CDS	30.0%
!!	AGAAAGTATTCGTATGTCAA+AGG	+	chr3.4:58973754-58973773	MS.gene04530:CDS	30.0%
!!	GAAAGTATTCGTATGTCAAA+GGG	+	chr3.4:58973755-58973774	MS.gene04530:CDS	30.0%
!!!	ACGGAAAAGTTTTGTTTCAA+GGG	+	chr3.4:58973405-58973424	MS.gene04530:CDS	30.0%
!!!	TATTTTTAGCCTTTTGTCAG+AGG	+	chr3.4:58973795-58973814	MS.gene04530:CDS	30.0%
	ATGCTTTAGCTGCTAACTAT+TGG	+	chr3.4:58973575-58973594	MS.gene04530:CDS	35.0%
	CTTGTATCCTAACCAAATCA+GGG	-	chr3.4:58973660-58973679	None:intergenic	35.0%
	GAGTGACAAACTATACTAGA+CGG	+	chr3.4:58973386-58973405	MS.gene04530:CDS	35.0%
	TGCTTTAGCTGCTAACTATT+GGG	+	chr3.4:58973576-58973595	MS.gene04530:CDS	35.0%
	TTCATATCAATTTCTCCAGC+TGG	+	chr3.4:58973511-58973530	MS.gene04530:CDS	35.0%
	TTTCAAGGATATCCCTGATT+TGG	+	chr3.4:58973645-58973664	MS.gene04530:CDS	35.0%
!	TGATGATGATGATAGGTGTA+GGG	+	chr3.4:58973540-58973559	MS.gene04530:CDS	35.0%
!	TGCTGTATCACAAGTTTTCA+AGG	+	chr3.4:58973630-58973649	MS.gene04530:CDS	35.0%
!!!	GACGGAAAAGTTTTGTTTCA+AGG	+	chr3.4:58973404-58973423	MS.gene04530:CDS	35.0%
!!!	GATACAAGCTTTTGTTTCTG+TGG	+	chr3.4:58973672-58973691	MS.gene04530:CDS	35.0%
	AATGAGTGTCCTCTGACAAA+AGG	-	chr3.4:58973807-58973826	None:intergenic	40.0%
	ACTTGTCAAGAATCACCTTG+CGG	+	chr3.4:58973311-58973330	MS.gene04530:CDS	40.0%
	ATACAGCAACTTTCACTGCT+TGG	-	chr3.4:58973618-58973637	None:intergenic	40.0%
	GACGATTTCATGTTAGTAGC+AGG	+	chr3.4:58973355-58973374	MS.gene04530:CDS	40.0%
	GCTTGTATCCTAACCAAATC+AGG	-	chr3.4:58973661-58973680	None:intergenic	40.0%
	GCTTTAGCTGCTAACTATTG+GGG	+	chr3.4:58973577-58973596	MS.gene04530:CDS	40.0%
	GTATGTCAAAGGGAGAGTTA+AGG	+	chr3.4:58973765-58973784	MS.gene04530:CDS	40.0%
	TACAGCAACTTTCACTGCTT+GGG	-	chr3.4:58973617-58973636	None:intergenic	40.0%
!	GATCTGGTGATGATGATGAT+AGG	+	chr3.4:58973533-58973552	MS.gene04530:CDS	40.0%
!	GTGATGATGATGATAGGTGT+AGG	+	chr3.4:58973539-58973558	MS.gene04530:CDS	40.0%
!!	AGGATATCCCTGATTTGGTT+AGG	+	chr3.4:58973650-58973669	MS.gene04530:CDS	40.0%
	AATGCATATGGATCGAGTCC+AGG	-	chr3.4:58973473-58973492	None:intergenic	45.0%
	ATGCATATGGATCGAGTCCA+GGG	-	chr3.4:58973472-58973491	None:intergenic	45.0%
	GGAAAAGGTTGGGTTCCTTA+GGG	+	chr3.4:58973720-58973739	MS.gene04530:CDS	45.0%
	GGAGAATCAAGCTTCTCTGA+GGG	+	chr3.4:58973693-58973712	MS.gene04530:CDS	45.0%
	TCAATTTCTCCAGCTGGATC+TGG	+	chr3.4:58973517-58973536	MS.gene04530:CDS	45.0%
	TCGACGTCACTCAATTCGAA+TGG	-	chr3.4:58973337-58973356	None:intergenic	45.0%
	TGAGGGTCTTGGAAAAGGTT+GGG	+	chr3.4:58973710-58973729	MS.gene04530:CDS	45.0%
	TGGAAAAGGTTGGGTTCCTT+AGG	+	chr3.4:58973719-58973738	MS.gene04530:CDS	45.0%
	TGGAGAATCAAGCTTCTCTG+AGG	+	chr3.4:58973692-58973711	MS.gene04530:CDS	45.0%
	TTCTGAGTATACCCTCCCTA+AGG	-	chr3.4:58973738-58973757	None:intergenic	45.0%
!	TTCTCTGAGGGTCTTGGAAA+AGG	+	chr3.4:58973705-58973724	MS.gene04530:CDS	45.0%
	AAAGGTTGGGTTCCTTAGGG+AGG	+	chr3.4:58973723-58973742	MS.gene04530:CDS	50.0%
	AAGGTTGGGTTCCTTAGGGA+GGG	+	chr3.4:58973724-58973743	MS.gene04530:CDS	50.0%
	ACTCAATTCGAATGGCCGCA+AGG	-	chr3.4:58973329-58973348	None:intergenic	50.0%
	CTGAGGGTCTTGGAAAAGGT+TGG	+	chr3.4:58973709-58973728	MS.gene04530:CDS	50.0%
	GATAGGTGTAGGGCTGAGAT+TGG	+	chr3.4:58973550-58973569	MS.gene04530:CDS	50.0%
	GCTGCTAACTATTGGGGTCA+AGG	+	chr3.4:58973583-58973602	MS.gene04530:CDS	50.0%
	GGTGAGGAATGCACACATCT+TGG	-	chr3.4:58973450-58973469	None:intergenic	50.0%
	TCATCATCACCAGATCCAGC+TGG	-	chr3.4:58973529-58973548	None:intergenic	50.0%
	TGCATATGGATCGAGTCCAG+GGG	-	chr3.4:58973471-58973490	None:intergenic	50.0%
!	TCAAGCTTCTCTGAGGGTCT+TGG	+	chr3.4:58973699-58973718	MS.gene04530:CDS	50.0%
	ATGTGTGCATTCCTCACCCC+TGG	+	chr3.4:58973452-58973471	MS.gene04530:CDS	55.0%
	ATGGATCGAGTCCAGGGGTG+AGG	-	chr3.4:58973466-58973485	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	58973301	58973837	58973301	ID=MS.gene04530
chr3.4	mRNA	58973301	58973837	58973301	ID=MS.gene04530.t1;Parent=MS.gene04530
chr3.4	exon	58973301	58973837	58973301	ID=MS.gene04530.t1.exon1;Parent=MS.gene04530.t1
chr3.4	CDS	58973301	58973837	58973301	ID=cds.MS.gene04530.t1;Parent=MS.gene04530.t1

Gene Sequence

>MS.gene04530

ATGGCGATGAACTTGTCAAGAATCACCTTGCGGCCATTCGAATTGAGTGACGTCGACGATTTCATGTTAGTAGCAGGTGATGATAGAGTGACAAACTATACTAGACGGAAAAGTTTTGTTTCAAGGGAACAAGCATTGTGTTACATCCAAGATGTGTGCATTCCTCACCCCTGGACTCGATCCATATGCATTGACAATCAAACTATCGGATTCATATCAATTTCTCCAGCTGGATCTGGTGATGATGATGATAGGTGTAGGGCTGAGATTGGCTATGCTTTAGCTGCTAACTATTGGGGTCAAGGTATATGCACCCAAGCAGTGAAAGTTGCTGTATCACAAGTTTTCAAGGATATCCCTGATTTGGTTAGGATACAAGCTTTTGTTTCTGTGGAGAATCAAGCTTCTCTGAGGGTCTTGGAAAAGGTTGGGTTCCTTAGGGAGGGTATACTCAGAAAGTATTCGTATGTCAAAGGGAGAGTTAAGGATGTAGCTATTTTTAGCCTTTTGTCAGAGGACACTCATTCTCTACAGTAA

Protein sequence

>MS.gene04530.t1

MAMNLSRITLRPFELSDVDDFMLVAGDDRVTNYTRRKSFVSREQALCYIQDVCIPHPWTRSICIDNQTIGFISISPAGSGDDDDRCRAEIGYALAANYWGQGICTQAVKVAVSQVFKDIPDLVRIQAFVSVENQASLRVLEKVGFLREGILRKYSYVKGRVKDVAIFSLLSEDTHSLQ