Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45776.t1 | XP_013445083.1 | 98.7 | 313 | 4 | 0 | 1 | 313 | 1 | 313 | 8.90E-169 | 602.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45776.t1 | P51074 | 68.2 | 314 | 99 | 1 | 1 | 313 | 1 | 314 | 2.8e-118 | 426.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45776.t1 | O65848 | 98.7 | 313 | 4 | 0 | 1 | 313 | 1 | 313 | 6.4e-169 | 602.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene45776 | -0.803322 | 3.68E-49 | -1.69E-46 |
| MS.gene049611 | MS.gene45776 | 0.814533 | 1.47E-51 | -1.69E-46 |
| MS.gene050170 | MS.gene45776 | -0.860439 | 2.06E-63 | -1.69E-46 |
| MS.gene050323 | MS.gene45776 | -0.819562 | 1.09E-52 | -1.69E-46 |
| MS.gene050338 | MS.gene45776 | 0.819134 | 1.36E-52 | -1.69E-46 |
| MS.gene050444 | MS.gene45776 | -0.810782 | 9.71E-51 | -1.69E-46 |
| MS.gene050496 | MS.gene45776 | -0.858092 | 1.04E-62 | -1.69E-46 |
| MS.gene050504 | MS.gene45776 | -0.819768 | 9.77E-53 | -1.69E-46 |
| MS.gene050577 | MS.gene45776 | -0.816742 | 4.73E-52 | -1.69E-46 |
| MS.gene050612 | MS.gene45776 | -0.804754 | 1.86E-49 | -1.69E-46 |
| MS.gene050675 | MS.gene45776 | -0.804644 | 1.96E-49 | -1.69E-46 |
| MS.gene050699 | MS.gene45776 | -0.806113 | 9.63E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene45776 | -0.838973 | 2.09E-57 | -1.69E-46 |
| MS.gene050871 | MS.gene45776 | -0.838677 | 2.49E-57 | -1.69E-46 |
| MS.gene050904 | MS.gene45776 | -0.803811 | 2.92E-49 | -1.69E-46 |
| MS.gene05104 | MS.gene45776 | 0.819956 | 8.85E-53 | -1.69E-46 |
| MS.gene051138 | MS.gene45776 | 0.821863 | 3.22E-53 | -1.69E-46 |
| MS.gene051268 | MS.gene45776 | -0.832866 | 7.40E-56 | -1.69E-46 |
| MS.gene051552 | MS.gene45776 | 0.8044 | 2.20E-49 | -1.69E-46 |
| MS.gene051651 | MS.gene45776 | 0.828993 | 6.60E-55 | -1.69E-46 |
| MS.gene051973 | MS.gene45776 | 0.816499 | 5.36E-52 | -1.69E-46 |
| MS.gene052129 | MS.gene45776 | 0.828123 | 1.07E-54 | -1.69E-46 |
| MS.gene052164 | MS.gene45776 | -0.814905 | 1.21E-51 | -1.69E-46 |
| MS.gene052494 | MS.gene45776 | 0.831781 | 1.37E-55 | -1.69E-46 |
| MS.gene052503 | MS.gene45776 | -0.816057 | 6.73E-52 | -1.69E-46 |
| MS.gene052606 | MS.gene45776 | 0.808808 | 2.58E-50 | -1.69E-46 |
| MS.gene052823 | MS.gene45776 | -0.824618 | 7.32E-54 | -1.69E-46 |
| MS.gene052825 | MS.gene45776 | -0.823203 | 1.57E-53 | -1.69E-46 |
| MS.gene053097 | MS.gene45776 | 0.839042 | 2.01E-57 | -1.69E-46 |
| MS.gene053203 | MS.gene45776 | -0.817039 | 4.05E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene45776 | 0.812769 | 3.59E-51 | -1.69E-46 |
| MS.gene053479 | MS.gene45776 | -0.830876 | 2.29E-55 | -1.69E-46 |
| MS.gene053824 | MS.gene45776 | -0.819182 | 1.33E-52 | -1.69E-46 |
| MS.gene054344 | MS.gene45776 | -0.818548 | 1.85E-52 | -1.69E-46 |
| MS.gene054706 | MS.gene45776 | 0.836844 | 7.37E-57 | -1.69E-46 |
| MS.gene05470 | MS.gene45776 | -0.855918 | 4.57E-62 | -1.69E-46 |
| MS.gene054954 | MS.gene45776 | -0.808543 | 2.94E-50 | -1.69E-46 |
| MS.gene055158 | MS.gene45776 | 0.823419 | 1.40E-53 | -1.69E-46 |
| MS.gene055251 | MS.gene45776 | 0.811646 | 6.31E-51 | -1.69E-46 |
| MS.gene055502 | MS.gene45776 | -0.800559 | 1.36E-48 | -1.69E-46 |
| MS.gene055577 | MS.gene45776 | -0.82485 | 6.45E-54 | -1.69E-46 |
| MS.gene055748 | MS.gene45776 | -0.816043 | 6.78E-52 | -1.69E-46 |
| MS.gene055751 | MS.gene45776 | -0.816829 | 4.52E-52 | -1.69E-46 |
| MS.gene05589 | MS.gene45776 | -0.861122 | 1.28E-63 | -1.69E-46 |
| MS.gene056198 | MS.gene45776 | 0.892139 | 2.09E-74 | -1.69E-46 |
| MS.gene056387 | MS.gene45776 | -0.86884 | 4.84E-66 | -1.69E-46 |
| MS.gene056504 | MS.gene45776 | -0.845215 | 4.67E-59 | -1.69E-46 |
| MS.gene056590 | MS.gene45776 | 0.805134 | 1.55E-49 | -1.69E-46 |
| MS.gene056591 | MS.gene45776 | 0.804677 | 1.93E-49 | -1.69E-46 |
| MS.gene056694 | MS.gene45776 | 0.808785 | 2.61E-50 | -1.69E-46 |
| MS.gene056697 | MS.gene45776 | 0.819474 | 1.14E-52 | -1.69E-46 |
| MS.gene056748 | MS.gene45776 | 0.821495 | 3.92E-53 | -1.69E-46 |
| MS.gene056856 | MS.gene45776 | 0.836179 | 1.09E-56 | -1.69E-46 |
| MS.gene056857 | MS.gene45776 | 0.805956 | 1.04E-49 | -1.69E-46 |
| MS.gene056988 | MS.gene45776 | 0.815439 | 9.24E-52 | -1.69E-46 |
| MS.gene057261 | MS.gene45776 | 0.805117 | 1.56E-49 | -1.69E-46 |
| MS.gene057658 | MS.gene45776 | -0.803164 | 3.97E-49 | -1.69E-46 |
| MS.gene057717 | MS.gene45776 | 0.831839 | 1.33E-55 | -1.69E-46 |
| MS.gene057768 | MS.gene45776 | 0.813396 | 2.61E-51 | -1.69E-46 |
| MS.gene057831 | MS.gene45776 | -0.800834 | 1.20E-48 | -1.69E-46 |
| MS.gene058068 | MS.gene45776 | 0.821395 | 4.13E-53 | -1.69E-46 |
| MS.gene058157 | MS.gene45776 | -0.820874 | 5.45E-53 | -1.69E-46 |
| MS.gene058268 | MS.gene45776 | 0.831683 | 1.45E-55 | -1.69E-46 |
| MS.gene058269 | MS.gene45776 | 0.82696 | 2.04E-54 | -1.69E-46 |
| MS.gene058334 | MS.gene45776 | 0.840836 | 6.84E-58 | -1.69E-46 |
| MS.gene05837 | MS.gene45776 | -0.80236 | 5.82E-49 | -1.69E-46 |
| MS.gene058382 | MS.gene45776 | 0.825374 | 4.85E-54 | -1.69E-46 |
| MS.gene058418 | MS.gene45776 | 0.836268 | 1.03E-56 | -1.69E-46 |
| MS.gene058452 | MS.gene45776 | 0.813348 | 2.68E-51 | -1.69E-46 |
| MS.gene058544 | MS.gene45776 | -0.808378 | 3.19E-50 | -1.69E-46 |
| MS.gene058603 | MS.gene45776 | 0.816643 | 4.98E-52 | -1.69E-46 |
| MS.gene058838 | MS.gene45776 | -0.808543 | 2.94E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene45776 | -0.814949 | 1.19E-51 | -1.69E-46 |
| MS.gene059023 | MS.gene45776 | -0.828322 | 9.59E-55 | -1.69E-46 |
| MS.gene059070 | MS.gene45776 | 0.800722 | 1.26E-48 | -1.69E-46 |
| MS.gene05926 | MS.gene45776 | 0.84624 | 2.46E-59 | -1.69E-46 |
| MS.gene059315 | MS.gene45776 | 0.806152 | 9.45E-50 | -1.69E-46 |
| MS.gene05960 | MS.gene45776 | 0.807082 | 6.01E-50 | -1.69E-46 |
| MS.gene059677 | MS.gene45776 | -0.814672 | 1.37E-51 | -1.69E-46 |
| MS.gene059703 | MS.gene45776 | 0.804856 | 1.77E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene45776 | -0.807759 | 4.32E-50 | -1.69E-46 |
| MS.gene06000 | MS.gene45776 | -0.855057 | 8.15E-62 | -1.69E-46 |
| MS.gene06027 | MS.gene45776 | -0.809785 | 1.59E-50 | -1.69E-46 |
| MS.gene060405 | MS.gene45776 | 0.851371 | 9.27E-61 | -1.69E-46 |
| MS.gene060762 | MS.gene45776 | -0.822288 | 2.57E-53 | -1.69E-46 |
| MS.gene060778 | MS.gene45776 | -0.832651 | 8.37E-56 | -1.69E-46 |
| MS.gene06077 | MS.gene45776 | -0.824781 | 6.70E-54 | -1.69E-46 |
| MS.gene060982 | MS.gene45776 | -0.830988 | 2.15E-55 | -1.69E-46 |
| MS.gene061044 | MS.gene45776 | 0.844285 | 8.31E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45776.t1 | MTR_8g038210 | 98.722 | 313 | 4 | 0 | 1 | 313 | 1 | 313 | 0.0 | 628 |
| MS.gene45776.t1 | MTR_8g038210 | 94.888 | 313 | 16 | 0 | 1 | 313 | 1 | 313 | 0.0 | 602 |
| MS.gene45776.t1 | MTR_8g038220 | 76.752 | 314 | 72 | 1 | 1 | 313 | 1 | 314 | 2.15e-178 | 495 |
| MS.gene45776.t1 | MTR_8g038220 | 75.796 | 314 | 74 | 2 | 1 | 313 | 1 | 313 | 1.14e-174 | 486 |
| MS.gene45776.t1 | MTR_3g018780 | 71.975 | 314 | 87 | 1 | 1 | 313 | 1 | 314 | 1.48e-166 | 465 |
| MS.gene45776.t1 | MTR_3g018790 | 68.790 | 314 | 97 | 1 | 1 | 313 | 1 | 314 | 5.80e-159 | 446 |
| MS.gene45776.t1 | MTR_8g038180 | 70.513 | 312 | 91 | 1 | 1 | 311 | 1 | 312 | 6.59e-158 | 443 |
| MS.gene45776.t1 | MTR_3g018790 | 67.769 | 242 | 77 | 1 | 73 | 313 | 19 | 260 | 5.15e-117 | 338 |
| MS.gene45776.t1 | MTR_0276s0050 | 51.429 | 315 | 151 | 2 | 1 | 313 | 1 | 315 | 2.31e-101 | 300 |
| MS.gene45776.t1 | MTR_2g031980 | 45.687 | 313 | 169 | 1 | 1 | 312 | 1 | 313 | 7.66e-99 | 293 |
| MS.gene45776.t1 | MTR_5g063670 | 47.436 | 312 | 162 | 2 | 1 | 310 | 1 | 312 | 2.03e-97 | 290 |
| MS.gene45776.t1 | MTR_3g018920 | 57.143 | 210 | 59 | 1 | 1 | 210 | 1 | 179 | 1.81e-79 | 239 |
| MS.gene45776.t1 | MTR_8g038150 | 40.823 | 316 | 182 | 2 | 1 | 311 | 1 | 316 | 7.65e-76 | 235 |
| MS.gene45776.t1 | MTR_8g107640 | 36.825 | 315 | 197 | 2 | 1 | 313 | 1 | 315 | 1.01e-68 | 217 |
| MS.gene45776.t1 | MTR_1g033560 | 37.460 | 315 | 193 | 4 | 1 | 312 | 1 | 314 | 5.04e-66 | 210 |
| MS.gene45776.t1 | MTR_6g071605 | 37.500 | 312 | 193 | 2 | 1 | 310 | 1 | 312 | 5.29e-65 | 207 |
| MS.gene45776.t1 | MTR_6g071615 | 37.179 | 312 | 194 | 2 | 1 | 310 | 1 | 312 | 2.05e-62 | 201 |
| MS.gene45776.t1 | MTR_1g112520 | 34.615 | 312 | 202 | 2 | 1 | 310 | 1 | 312 | 3.98e-58 | 189 |
| MS.gene45776.t1 | MTR_6g071595 | 35.235 | 298 | 192 | 1 | 14 | 310 | 15 | 312 | 4.11e-56 | 184 |
| MS.gene45776.t1 | MTR_8g038170 | 36.392 | 316 | 175 | 7 | 13 | 310 | 4 | 311 | 3.77e-50 | 169 |
| MS.gene45776.t1 | MTR_8g038170 | 36.392 | 316 | 175 | 7 | 13 | 310 | 4 | 311 | 3.77e-50 | 169 |
| MS.gene45776.t1 | MTR_3g018925 | 70.103 | 97 | 29 | 0 | 217 | 313 | 13 | 109 | 3.65e-40 | 136 |
| MS.gene45776.t1 | MTR_6g071595 | 33.191 | 235 | 156 | 1 | 14 | 247 | 15 | 249 | 1.38e-39 | 140 |
| MS.gene45776.t1 | MTR_6g028030 | 26.923 | 312 | 211 | 7 | 8 | 308 | 2 | 307 | 2.80e-27 | 108 |
| MS.gene45776.t1 | MTR_8g038090 | 50.000 | 72 | 31 | 2 | 243 | 311 | 22 | 91 | 4.72e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45776.t1 | AT5G12380 | 54.984 | 311 | 139 | 1 | 1 | 310 | 1 | 311 | 9.92e-124 | 357 |
| MS.gene45776.t1 | AT5G10230 | 51.592 | 314 | 150 | 2 | 1 | 312 | 1 | 314 | 1.95e-106 | 313 |
| MS.gene45776.t1 | AT1G35720 | 50.789 | 317 | 151 | 4 | 1 | 313 | 1 | 316 | 2.11e-106 | 313 |
| MS.gene45776.t1 | AT5G10220 | 50.316 | 316 | 153 | 3 | 1 | 312 | 1 | 316 | 9.37e-102 | 301 |
| MS.gene45776.t1 | AT5G65020 | 50.799 | 313 | 151 | 3 | 1 | 310 | 1 | 313 | 1.65e-101 | 300 |
| MS.gene45776.t1 | AT5G65020 | 50.347 | 288 | 141 | 2 | 25 | 310 | 11 | 298 | 1.45e-92 | 277 |
| MS.gene45776.t1 | AT2G38760 | 41.509 | 318 | 178 | 3 | 1 | 310 | 1 | 318 | 8.71e-79 | 243 |
| MS.gene45776.t1 | AT1G68090 | 35.463 | 313 | 199 | 3 | 1 | 310 | 1 | 313 | 1.94e-59 | 193 |
| MS.gene45776.t1 | AT2G38750 | 33.442 | 308 | 179 | 7 | 25 | 313 | 19 | 319 | 5.27e-43 | 150 |
| MS.gene45776.t1 | AT2G38750 | 33.442 | 308 | 179 | 7 | 25 | 313 | 2 | 302 | 6.45e-43 | 150 |
| MS.gene45776.t1 | AT1G68090 | 34.400 | 250 | 161 | 3 | 1 | 247 | 1 | 250 | 3.27e-42 | 146 |
Find 68 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTCAGTTTGTGCCAATTT+TGG | 0.171052 | 8.3:-58641714 | None:intergenic |
| TTAATCTTTACAGAAGCTTT+TGG | 0.285693 | 8.3:+58641945 | MS.gene45776:intron |
| TGGGGCTAGTGACTGCATTT+AGG | 0.296540 | 8.3:+58641670 | MS.gene45776:CDS |
| GCACTCCTTGGAGGAAGATT+TGG | 0.307016 | 8.3:+58641440 | MS.gene45776:CDS |
| TGATGAGATCAATCCAAAAT+TGG | 0.309418 | 8.3:+58641701 | MS.gene45776:CDS |
| CGTAAGCACGTCTCACATTA+AGG | 0.309809 | 8.3:-58641404 | None:intergenic |
| TGCGGAACGTGATGCTGTTT+TGG | 0.338707 | 8.3:+58641305 | MS.gene45776:CDS |
| CTCACATTAAGGAGCTCTTC+GGG | 0.341349 | 8.3:-58641393 | None:intergenic |
| AAGTTTCTTCTCACTCTCTT+GGG | 0.347125 | 8.3:+58642419 | MS.gene45776:CDS |
| CCTTGTAGTCAAGATCCTTA+TGG | 0.357139 | 8.3:-58641787 | None:intergenic |
| CAGCATCTTCATTGGGAGAA+TGG | 0.357218 | 8.3:-58640758 | None:intergenic |
| AATGGTTGCTGGGAGCAATA+AGG | 0.357762 | 8.3:-58640740 | None:intergenic |
| GAAGTTTCTTCTCACTCTCT+TGG | 0.372788 | 8.3:+58642418 | MS.gene45776:CDS |
| GTAATCCCCTGAAGTTTCCT+TGG | 0.400457 | 8.3:-58642393 | None:intergenic |
| GTTGTGGCCAAGGAAACTTC+AGG | 0.410461 | 8.3:+58642386 | MS.gene45776:CDS |
| TAGAGCTTCAGCATCTTCAT+TGG | 0.413067 | 8.3:-58640766 | None:intergenic |
| AGATCCTTATGGCTTCTTCA+TGG | 0.424810 | 8.3:-58641776 | None:intergenic |
| TCTCACATTAAGGAGCTCTT+CGG | 0.434874 | 8.3:-58641394 | None:intergenic |
| TTCATTGGGAGAATGGTTGC+TGG | 0.439937 | 8.3:-58640751 | None:intergenic |
| AGAGCTTCAGCATCTTCATT+GGG | 0.446820 | 8.3:-58640765 | None:intergenic |
| AAGGACTTGCCTGGCGAAGA+TGG | 0.449423 | 8.3:-58641479 | None:intergenic |
| TGAGATCAAAGGAGACTTTG+AGG | 0.477521 | 8.3:+58641130 | MS.gene45776:CDS |
| ATGGTTGCTGGGAGCAATAA+GGG | 0.478955 | 8.3:-58640739 | None:intergenic |
| TACCTCTGGCCATCTTCGCC+AGG | 0.485111 | 8.3:+58641470 | MS.gene45776:CDS |
| TCTTGAGCATGTTGTGGCCA+AGG | 0.497223 | 8.3:+58642376 | MS.gene45776:CDS |
| TGAGGATCTTATCAAACGAT+TGG | 0.500715 | 8.3:+58641103 | MS.gene45776:CDS |
| AAATTGGCACAAACTGAAGC+TGG | 0.505178 | 8.3:+58641717 | MS.gene45776:CDS |
| CAAACGCTACAAGCACTCCT+TGG | 0.517630 | 8.3:+58641428 | MS.gene45776:CDS |
| CCATAAGGATCTTGACTACA+AGG | 0.517780 | 8.3:+58641787 | MS.gene45776:CDS |
| GATTTGGCTGCACATACCTC+TGG | 0.527814 | 8.3:+58641456 | MS.gene45776:CDS |
| CGAGATGCAATCAAGAAGGT+TGG | 0.532695 | 8.3:+58642257 | MS.gene45776:CDS |
| GTGACTGCATTTAGGTATGT+TGG | 0.533407 | 8.3:+58641678 | MS.gene45776:CDS |
| AGTTTCTTCTCACTCTCTTG+GGG | 0.540899 | 8.3:+58642420 | MS.gene45776:CDS |
| GAAGCCATGAAGAAGCCATA+AGG | 0.541126 | 8.3:+58641772 | MS.gene45776:CDS |
| CAAATCAGAAAAGCTTATGA+AGG | 0.541965 | 8.3:+58641071 | MS.gene45776:CDS |
| TCATGTTAAGTACTATGAAA+AGG | 0.546698 | 8.3:+58642026 | MS.gene45776:CDS |
| ACGCTACAAGCACTCCTTGG+AGG | 0.551063 | 8.3:+58641431 | MS.gene45776:CDS |
| GAGTCAGTGAAAGAGAAGAA+AGG | 0.551826 | 8.3:+58641750 | MS.gene45776:CDS |
| AAAACTGTGATAACAATACT+TGG | 0.555304 | 8.3:+58641023 | MS.gene45776:CDS |
| TTGTGGCCAAGGAAACTTCA+GGG | 0.559821 | 8.3:+58642387 | MS.gene45776:CDS |
| TTTATTATATACCTTAGTGA+TGG | 0.564240 | 8.3:-58641865 | None:intergenic |
| TCATTGGGAGAATGGTTGCT+GGG | 0.565172 | 8.3:-58640750 | None:intergenic |
| TGTTCTTCTTGAGCATGTTG+TGG | 0.565750 | 8.3:+58642370 | MS.gene45776:CDS |
| TCATGGTACTTCCATCACTA+AGG | 0.567292 | 8.3:+58641854 | MS.gene45776:CDS |
| AGATGCTGAAGCTCTAAGAA+AGG | 0.567867 | 8.3:+58640773 | MS.gene45776:CDS |
| ACGGGTGAGCGCATCCTCAT+CGG | 0.568327 | 8.3:-58642282 | None:intergenic |
| TTATGAAGGAATTTATAATG+AGG | 0.573543 | 8.3:+58641085 | MS.gene45776:CDS |
| AAAACAGCATCACGTTCCGC+AGG | 0.584641 | 8.3:-58641303 | None:intergenic |
| AACAGAAAGCTGTGTACCGT+TGG | 0.585865 | 8.3:+58641271 | MS.gene45776:intron |
| GCAAATGTTGCTATCAAGAG+TGG | 0.587800 | 8.3:+58641327 | MS.gene45776:CDS |
| CGATTGGAATCTGAGATCAA+AGG | 0.597023 | 8.3:+58641119 | MS.gene45776:CDS |
| TGCAGCCAAATCTTCCTCCA+AGG | 0.611461 | 8.3:-58641445 | None:intergenic |
| CACATTAAGGAGCTCTTCGG+GGG | 0.612141 | 8.3:-58641391 | None:intergenic |
| CCGTTGGATACTTGAACCTG+CGG | 0.614913 | 8.3:+58641287 | MS.gene45776:CDS |
| GAGCTCTGCTCACAATCACA+CGG | 0.617837 | 8.3:-58642301 | None:intergenic |
| GCTCTAAGAAAGGCTTTCGA+AGG | 0.621766 | 8.3:+58640783 | MS.gene45776:CDS |
| ATGATCATTGAAGCAACGAA+TGG | 0.624429 | 8.3:-58642007 | None:intergenic |
| AGGATCTGATGAATTTCAGA+AGG | 0.633929 | 8.3:+58641972 | MS.gene45776:CDS |
| TACCATGAGTCTCTCTGTAG+CGG | 0.635249 | 8.3:-58641839 | None:intergenic |
| GGTGCGAGATGCAATCAAGA+AGG | 0.643809 | 8.3:+58642253 | MS.gene45776:CDS |
| TCACATTAAGGAGCTCTTCG+GGG | 0.651841 | 8.3:-58641392 | None:intergenic |
| CAAGAAGGTTGGAACCGATG+AGG | 0.660964 | 8.3:+58642268 | MS.gene45776:CDS |
| GTTTCTTCTCACTCTCTTGG+GGG | 0.667702 | 8.3:+58642421 | MS.gene45776:CDS |
| AGCTCTGCTCACAATCACAC+GGG | 0.668833 | 8.3:-58642300 | None:intergenic |
| CCGCAGGTTCAAGTATCCAA+CGG | 0.670302 | 8.3:-58641287 | None:intergenic |
| TGTGGCCAAGGAAACTTCAG+GGG | 0.678511 | 8.3:+58642388 | MS.gene45776:CDS |
| TGCCTGGCGAAGATGGCCAG+AGG | 0.681136 | 8.3:-58641472 | None:intergenic |
| AACCGCTACAGAGAGACTCA+TGG | 0.682685 | 8.3:+58641837 | MS.gene45776:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGTTGTATTAAAATTGA+TGG | + | chr8.3:58640966-58640985 | MS.gene45776:intron | 15.0% |
| !! | ATCTATAAACAAGAAAAAAG+GGG | - | chr8.3:58640818-58640837 | None:intergenic | 20.0% |
| !! | CAATATGCAATATTATATAG+TGG | + | chr8.3:58641521-58641540 | MS.gene45776:intron | 20.0% |
| !! | CGATTACATATATGAAAATT+AGG | + | chr8.3:58641573-58641592 | MS.gene45776:intron | 20.0% |
| !! | GATCTATAAACAAGAAAAAA+GGG | - | chr8.3:58640819-58640838 | None:intergenic | 20.0% |
| !! | TGATCTATAAACAAGAAAAA+AGG | - | chr8.3:58640820-58640839 | None:intergenic | 20.0% |
| !! | TTATGAAGGAATTTATAATG+AGG | + | chr8.3:58641085-58641104 | MS.gene45776:CDS | 20.0% |
| !! | TTTATTATATACCTTAGTGA+TGG | - | chr8.3:58641868-58641887 | None:intergenic | 20.0% |
| !!! | ACATTTTATGATCATATTGT+AGG | + | chr8.3:58642229-58642248 | MS.gene45776:intron | 20.0% |
| !!! | ATGTTTTTCAATGTTAATCA+TGG | - | chr8.3:58641158-58641177 | None:intergenic | 20.0% |
| ! | AAAACTGTGATAACAATACT+TGG | + | chr8.3:58641023-58641042 | MS.gene45776:CDS | 25.0% |
| ! | AAAGCTTCTGTAAAGATTAA+AGG | - | chr8.3:58641945-58641964 | None:intergenic | 25.0% |
| ! | AAGCTTCTGTAAAGATTAAA+GGG | - | chr8.3:58641944-58641963 | None:intergenic | 25.0% |
| ! | AGATATAATCTATAGGACTA+TGG | - | chr8.3:58640845-58640864 | None:intergenic | 25.0% |
| ! | CATATTGTTAGAAGAGTTAA+TGG | - | chr8.3:58641508-58641527 | None:intergenic | 25.0% |
| ! | TCATGACAGATATAATCTAT+AGG | - | chr8.3:58640852-58640871 | None:intergenic | 25.0% |
| ! | TCATGTTAAGTACTATGAAA+AGG | + | chr8.3:58642026-58642045 | MS.gene45776:CDS | 25.0% |
| ! | TGTGATATTTCTTGAAATCA+TGG | + | chr8.3:58641608-58641627 | MS.gene45776:intron | 25.0% |
| ! | TTAGAAGAGTTAATGGAAAA+AGG | - | chr8.3:58641501-58641520 | None:intergenic | 25.0% |
| !! | CTAGCTAAACATTTTATGAA+TGG | - | chr8.3:58642134-58642153 | None:intergenic | 25.0% |
| !! | TTAATCTTTACAGAAGCTTT+TGG | + | chr8.3:58641945-58641964 | MS.gene45776:intron | 25.0% |
| !! | TTTTATGATCATATTGTAGG+TGG | + | chr8.3:58642232-58642251 | MS.gene45776:intron | 25.0% |
| !!! | ATATAGTGGTTTTAAAATCG+CGG | + | chr8.3:58641535-58641554 | MS.gene45776:intron | 25.0% |
| !!! | ATGATTCATTTTTGTAGGAT+GGG | + | chr8.3:58640989-58641008 | MS.gene45776:intron | 25.0% |
| CAAAAACTACAATGTCATTG+TGG | + | chr8.3:58641350-58641369 | MS.gene45776:CDS | 30.0% | |
| CAAATCAGAAAAGCTTATGA+AGG | + | chr8.3:58641071-58641090 | MS.gene45776:CDS | 30.0% | |
| TGATGAGATCAATCCAAAAT+TGG | + | chr8.3:58641701-58641720 | MS.gene45776:CDS | 30.0% | |
| ! | ATCATGGTTTCTTGATTTTG+TGG | + | chr8.3:58641624-58641643 | MS.gene45776:intron | 30.0% |
| !! | AAACATCTGAGATGACTTTT+AGG | - | chr8.3:58642334-58642353 | None:intergenic | 30.0% |
| !! | GATGATTCATTTTTGTAGGA+TGG | + | chr8.3:58640988-58641007 | MS.gene45776:intron | 30.0% |
| !! | GATGGATGATTCATTTTTGT+AGG | + | chr8.3:58640984-58641003 | MS.gene45776:intron | 30.0% |
| !! | TGATTCATTTTTGTAGGATG+GGG | + | chr8.3:58640990-58641009 | MS.gene45776:intron | 30.0% |
| AAGTTTCTTCTCACTCTCTT+GGG | + | chr8.3:58642419-58642438 | MS.gene45776:CDS | 35.0% | |
| AGGATCTGATGAATTTCAGA+AGG | + | chr8.3:58641972-58641991 | MS.gene45776:CDS | 35.0% | |
| ATGATCATTGAAGCAACGAA+TGG | - | chr8.3:58642010-58642029 | None:intergenic | 35.0% | |
| TGAGGATCTTATCAAACGAT+TGG | + | chr8.3:58641103-58641122 | MS.gene45776:CDS | 35.0% | |
| ! | TTACAGAAGCTTTTGGATGA+AGG | + | chr8.3:58641952-58641971 | MS.gene45776:intron | 35.0% |
| !!! | ATTTTTCGAGCAGCTTTTAG+TGG | + | chr8.3:58641650-58641669 | MS.gene45776:intron | 35.0% |
| !!! | TTTTTCGAGCAGCTTTTAGT+GGG | + | chr8.3:58641651-58641670 | MS.gene45776:intron | 35.0% |
| AAATTGGCACAAACTGAAGC+TGG | + | chr8.3:58641717-58641736 | MS.gene45776:CDS | 40.0% | |
| AGAGCTTCAGCATCTTCATT+GGG | - | chr8.3:58640768-58640787 | None:intergenic | 40.0% | |
| AGATCCTTATGGCTTCTTCA+TGG | - | chr8.3:58641779-58641798 | None:intergenic | 40.0% | |
| AGTTTCTTCTCACTCTCTTG+GGG | + | chr8.3:58642420-58642439 | MS.gene45776:CDS | 40.0% | |
| CCATAAGGATCTTGACTACA+AGG | + | chr8.3:58641787-58641806 | MS.gene45776:CDS | 40.0% | |
| CCTTGTAGTCAAGATCCTTA+TGG | - | chr8.3:58641790-58641809 | None:intergenic | 40.0% | |
| GAAGTTTCTTCTCACTCTCT+TGG | + | chr8.3:58642418-58642437 | MS.gene45776:CDS | 40.0% | |
| GAGTCAGTGAAAGAGAAGAA+AGG | + | chr8.3:58641750-58641769 | MS.gene45776:CDS | 40.0% | |
| GCAAATGTTGCTATCAAGAG+TGG | + | chr8.3:58641327-58641346 | MS.gene45776:CDS | 40.0% | |
| GTGACTGCATTTAGGTATGT+TGG | + | chr8.3:58641678-58641697 | MS.gene45776:CDS | 40.0% | |
| TAATGGAAAAAGGACTTGCC+TGG | - | chr8.3:58641491-58641510 | None:intergenic | 40.0% | |
| TAGAGCTTCAGCATCTTCAT+TGG | - | chr8.3:58640769-58640788 | None:intergenic | 40.0% | |
| TCATGGTACTTCCATCACTA+AGG | + | chr8.3:58641854-58641873 | MS.gene45776:CDS | 40.0% | |
| TCTCACATTAAGGAGCTCTT+CGG | - | chr8.3:58641397-58641416 | None:intergenic | 40.0% | |
| TGAGATCAAAGGAGACTTTG+AGG | + | chr8.3:58641130-58641149 | MS.gene45776:CDS | 40.0% | |
| TGTTCTTCTTGAGCATGTTG+TGG | + | chr8.3:58642370-58642389 | MS.gene45776:CDS | 40.0% | |
| ! | CGATTGGAATCTGAGATCAA+AGG | + | chr8.3:58641119-58641138 | MS.gene45776:CDS | 40.0% |
| ! | GCTTCAGTTTGTGCCAATTT+TGG | - | chr8.3:58641717-58641736 | None:intergenic | 40.0% |
| !! | AGATGCTGAAGCTCTAAGAA+AGG | + | chr8.3:58640773-58640792 | MS.gene45776:CDS | 40.0% |
| !! | TTTTCGAGCAGCTTTTAGTG+GGG | + | chr8.3:58641652-58641671 | MS.gene45776:intron | 40.0% |
| AACAGAAAGCTGTGTACCGT+TGG | + | chr8.3:58641271-58641290 | MS.gene45776:intron | 45.0% | |
| AATGGTTGCTGGGAGCAATA+AGG | - | chr8.3:58640743-58640762 | None:intergenic | 45.0% | |
| ATGGTTGCTGGGAGCAATAA+GGG | - | chr8.3:58640742-58640761 | None:intergenic | 45.0% | |
| CAGCATCTTCATTGGGAGAA+TGG | - | chr8.3:58640761-58640780 | None:intergenic | 45.0% | |
| CGAGATGCAATCAAGAAGGT+TGG | + | chr8.3:58642257-58642276 | MS.gene45776:CDS | 45.0% | |
| CGTAAGCACGTCTCACATTA+AGG | - | chr8.3:58641407-58641426 | None:intergenic | 45.0% | |
| CTCACATTAAGGAGCTCTTC+GGG | - | chr8.3:58641396-58641415 | None:intergenic | 45.0% | |
| GAAGCCATGAAGAAGCCATA+AGG | + | chr8.3:58641772-58641791 | MS.gene45776:CDS | 45.0% | |
| GTAATCCCCTGAAGTTTCCT+TGG | - | chr8.3:58642396-58642415 | None:intergenic | 45.0% | |
| GTTTCTTCTCACTCTCTTGG+GGG | + | chr8.3:58642421-58642440 | MS.gene45776:CDS | 45.0% | |
| TACCATGAGTCTCTCTGTAG+CGG | - | chr8.3:58641842-58641861 | None:intergenic | 45.0% | |
| TCACATTAAGGAGCTCTTCG+GGG | - | chr8.3:58641395-58641414 | None:intergenic | 45.0% | |
| TCATTGGGAGAATGGTTGCT+GGG | - | chr8.3:58640753-58640772 | None:intergenic | 45.0% | |
| TTCATTGGGAGAATGGTTGC+TGG | - | chr8.3:58640754-58640773 | None:intergenic | 45.0% | |
| TTGTGGCCAAGGAAACTTCA+GGG | + | chr8.3:58642387-58642406 | MS.gene45776:CDS | 45.0% | |
| !! | GCTCTAAGAAAGGCTTTCGA+AGG | + | chr8.3:58640783-58640802 | MS.gene45776:CDS | 45.0% |
| AAAACAGCATCACGTTCCGC+AGG | - | chr8.3:58641306-58641325 | None:intergenic | 50.0% | |
| AACCGCTACAGAGAGACTCA+TGG | + | chr8.3:58641837-58641856 | MS.gene45776:CDS | 50.0% | |
| AGCTCTGCTCACAATCACAC+GGG | - | chr8.3:58642303-58642322 | None:intergenic | 50.0% | |
| CAAACGCTACAAGCACTCCT+TGG | + | chr8.3:58641428-58641447 | MS.gene45776:CDS | 50.0% | |
| CAAGAAGGTTGGAACCGATG+AGG | + | chr8.3:58642268-58642287 | MS.gene45776:CDS | 50.0% | |
| CACATTAAGGAGCTCTTCGG+GGG | - | chr8.3:58641394-58641413 | None:intergenic | 50.0% | |
| CCGCAGGTTCAAGTATCCAA+CGG | - | chr8.3:58641290-58641309 | None:intergenic | 50.0% | |
| CCGTTGGATACTTGAACCTG+CGG | + | chr8.3:58641287-58641306 | MS.gene45776:CDS | 50.0% | |
| GAGCTCTGCTCACAATCACA+CGG | - | chr8.3:58642304-58642323 | None:intergenic | 50.0% | |
| GATTTGGCTGCACATACCTC+TGG | + | chr8.3:58641456-58641475 | MS.gene45776:CDS | 50.0% | |
| GCACTCCTTGGAGGAAGATT+TGG | + | chr8.3:58641440-58641459 | MS.gene45776:CDS | 50.0% | |
| GGTGCGAGATGCAATCAAGA+AGG | + | chr8.3:58642253-58642272 | MS.gene45776:CDS | 50.0% | |
| GTTGTGGCCAAGGAAACTTC+AGG | + | chr8.3:58642386-58642405 | MS.gene45776:CDS | 50.0% | |
| TCTTGAGCATGTTGTGGCCA+AGG | + | chr8.3:58642376-58642395 | MS.gene45776:CDS | 50.0% | |
| TGCAGCCAAATCTTCCTCCA+AGG | - | chr8.3:58641448-58641467 | None:intergenic | 50.0% | |
| TGTGGCCAAGGAAACTTCAG+GGG | + | chr8.3:58642388-58642407 | MS.gene45776:CDS | 50.0% | |
| ! | TGGGGCTAGTGACTGCATTT+AGG | + | chr8.3:58641670-58641689 | MS.gene45776:CDS | 50.0% |
| !! | TGCGGAACGTGATGCTGTTT+TGG | + | chr8.3:58641305-58641324 | MS.gene45776:CDS | 50.0% |
| AAGGACTTGCCTGGCGAAGA+TGG | - | chr8.3:58641482-58641501 | None:intergenic | 55.0% | |
| ACGCTACAAGCACTCCTTGG+AGG | + | chr8.3:58641431-58641450 | MS.gene45776:CDS | 55.0% | |
| ACGGGTGAGCGCATCCTCAT+CGG | - | chr8.3:58642285-58642304 | None:intergenic | 60.0% | |
| TACCTCTGGCCATCTTCGCC+AGG | + | chr8.3:58641470-58641489 | MS.gene45776:CDS | 60.0% | |
| TGCCTGGCGAAGATGGCCAG+AGG | - | chr8.3:58641475-58641494 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 58640732 | 58642454 | 58640732 | ID=MS.gene45776 |
| chr8.3 | mRNA | 58640732 | 58642454 | 58640732 | ID=MS.gene45776.t1;Parent=MS.gene45776 |
| chr8.3 | exon | 58640732 | 58640804 | 58640732 | ID=MS.gene45776.t1.exon1;Parent=MS.gene45776.t1 |
| chr8.3 | CDS | 58640732 | 58640804 | 58640732 | ID=cds.MS.gene45776.t1;Parent=MS.gene45776.t1 |
| chr8.3 | exon | 58641006 | 58641151 | 58641006 | ID=MS.gene45776.t1.exon2;Parent=MS.gene45776.t1 |
| chr8.3 | CDS | 58641006 | 58641151 | 58641006 | ID=cds.MS.gene45776.t1;Parent=MS.gene45776.t1 |
| chr8.3 | exon | 58641276 | 58641491 | 58641276 | ID=MS.gene45776.t1.exon3;Parent=MS.gene45776.t1 |
| chr8.3 | CDS | 58641276 | 58641491 | 58641276 | ID=cds.MS.gene45776.t1;Parent=MS.gene45776.t1 |
| chr8.3 | exon | 58641663 | 58641875 | 58641663 | ID=MS.gene45776.t1.exon4;Parent=MS.gene45776.t1 |
| chr8.3 | CDS | 58641663 | 58641875 | 58641663 | ID=cds.MS.gene45776.t1;Parent=MS.gene45776.t1 |
| chr8.3 | exon | 58641958 | 58642047 | 58641958 | ID=MS.gene45776.t1.exon5;Parent=MS.gene45776.t1 |
| chr8.3 | CDS | 58641958 | 58642047 | 58641958 | ID=cds.MS.gene45776.t1;Parent=MS.gene45776.t1 |
| chr8.3 | exon | 58642251 | 58642454 | 58642251 | ID=MS.gene45776.t1.exon6;Parent=MS.gene45776.t1 |
| chr8.3 | CDS | 58642251 | 58642454 | 58642251 | ID=cds.MS.gene45776.t1;Parent=MS.gene45776.t1 |
| Gene Sequence |
| Protein sequence |
