AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44058


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGTAGCAGTCCCTCTGGC+AGG 0.468310 1.3:-57947055 MS.gene44058:CDS
TTTCAGATTCGTACCTTCCT+CGG 0.487984 1.3:+57946974 None:intergenic
CTCTGATGGTATAGCTACAA+AGG 0.532244 1.3:-57947267 MS.gene44058:CDS
ATATTTGACTTCTCCGAGGA+AGG 0.549457 1.3:-57946987 None:intergenic
GTGGATTGTAGCAGTCCCTC+TGG 0.554454 1.3:-57947059 MS.gene44058:CDS
GGTGCCTCAAATTTCTCTGA+TGG 0.559962 1.3:-57947281 MS.gene44058:CDS
TATACCATCAGAGAAATTTG+AGG 0.562160 1.3:+57947277 None:intergenic
ATCAGATTTCTCCCTGCCAG+AGG 0.577582 1.3:+57947043 None:intergenic
TCAGATTTCTCCCTGCCAGA+GGG 0.589490 1.3:+57947044 None:intergenic
AGCAGAAGTTACTACTGATG+TGG 0.591016 1.3:-57947078 MS.gene44058:CDS
TTGTAGCAGTCCCTCTGGCA+GGG 0.602924 1.3:-57947054 MS.gene44058:CDS
GGTGATAATGATGACATGCT+TGG 0.621093 1.3:-57947014 MS.gene44058:CDS
AGGGAGAAATCTGATGAAGA+TGG 0.631588 1.3:-57947035 MS.gene44058:CDS
AAGGTTAAATCATCAGAACA+TGG 0.666854 1.3:-57947248 MS.gene44058:CDS
GAATATGCATGTTACTTGTG+AGG 0.697394 1.3:-57947180 MS.gene44058:CDS
TGATATATTTGACTTCTCCG+AGG 0.718503 1.3:-57946991 MS.gene44058:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTCAATTCAAGTTTTAG+TGG - chr1.3:57947056-57947075 MS.gene44058:CDS 20.0%
!!! TATGATCAATTTTTTCAAGA+GGG - chr1.3:57947134-57947153 MS.gene44058:CDS 20.0%
! TGAAAAAATTGATCATAGTC+TGG + chr1.3:57947131-57947150 None:intergenic 25.0%
!!! CTATGATCAATTTTTTCAAG+AGG - chr1.3:57947133-57947152 MS.gene44058:CDS 25.0%
AAGGTTAAATCATCAGAACA+TGG - chr1.3:57947020-57947039 MS.gene44058:CDS 30.0%
TATACCATCAGAGAAATTTG+AGG + chr1.3:57946994-57947013 None:intergenic 30.0%
! GAATATGCATGTTACTTGTG+AGG - chr1.3:57947088-57947107 MS.gene44058:CDS 35.0%
! TGATATATTTGACTTCTCCG+AGG - chr1.3:57947277-57947296 MS.gene44058:CDS 35.0%
AGCAGAAGTTACTACTGATG+TGG - chr1.3:57947190-57947209 MS.gene44058:CDS 40.0%
AGGGAGAAATCTGATGAAGA+TGG - chr1.3:57947233-57947252 MS.gene44058:CDS 40.0%
CTCTGATGGTATAGCTACAA+AGG - chr1.3:57947001-57947020 MS.gene44058:CDS 40.0%
! ATATTTGACTTCTCCGAGGA+AGG - chr1.3:57947281-57947300 MS.gene44058:CDS 40.0%
! GGTGATAATGATGACATGCT+TGG - chr1.3:57947254-57947273 MS.gene44058:CDS 40.0%
ATCAGATTTCTCCCTGCCAG+AGG + chr1.3:57947228-57947247 None:intergenic 50.0%
TCAGATTTCTCCCTGCCAGA+GGG + chr1.3:57947227-57947246 None:intergenic 50.0%
ATTGTAGCAGTCCCTCTGGC+AGG - chr1.3:57947213-57947232 MS.gene44058:CDS 55.0%
TTGTAGCAGTCCCTCTGGCA+GGG - chr1.3:57947214-57947233 MS.gene44058:CDS 55.0%
! GTGGATTGTAGCAGTCCCTC+TGG - chr1.3:57947209-57947228 MS.gene44058:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 57946988 57947302 57946988 ID=MS.gene44058
chr1.3 mRNA 57946988 57947302 57946988 ID=MS.gene44058.t1;Parent=MS.gene44058
chr1.3 exon 57946988 57947302 57946988 ID=MS.gene44058.t1.exon1;Parent=MS.gene44058.t1
chr1.3 CDS 57946988 57947302 57946988 ID=cds.MS.gene44058.t1;Parent=MS.gene44058.t1
Gene Sequence

>MS.gene44058

GTGCCTCAAATTTCTCTGATGGTATAGCTACAAAGGTTAAATCATCAGAACATGGAAAAGTCAGTGACAATTTCAATTCAAGTTTTAGTGGTTCAGATATGAATATGCATGTTACTTGTGAGGAGTCAATACGTGACTCGCCAGACTATGATCAATTTTTTCAAGAGGGTTATTGTAAAGCATCAGTTGACTGTCATGAATCAGCAGAAGTTACTACTGATGTGGATTGTAGCAGTCCCTCTGGCAGGGAGAAATCTGATGAAGATGGTGATAATGATGACATGCTTGGTGATATATTTGACTTCTCCGAGGAAG

Protein sequence

>MS.gene44058.t1

VPQISLMVULQRLNHQNMEKSVTISIQVLVVQIUICMLLVRSQYVTRQTMINFFKRVIVKHQLTVMNQQKLLLMWIVAVPLAGRNLMKMVIMMTCLVIYLTSPRK