AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71534


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 31 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATGTGGCACAAGAGTTTA+AGG 0.257673 7.2:+88607060 MS.gene71534:intron
AATCGATGTTGATGAATTAT+CGG 0.348841 7.2:+88606453 MS.gene71534:CDS
TGTGGTTGACTTTACTTGCT+TGG 0.384738 7.2:-88606054 None:intergenic
AAAGAGGTTGATAAGGTTGT+TGG 0.418770 7.2:+88607121 MS.gene71534:CDS
GCTGTCTTTGTGCTGAATGT+TGG 0.441277 7.2:-88606086 None:intergenic
TTAGCCATGGCTTGAAGAAT+CGG 0.442279 7.2:-88606403 None:intergenic
CGTTTGTGTTGTTGAAGAAT+GGG 0.453169 7.2:+88607098 MS.gene71534:CDS
ACGTTTGTGTTGTTGAAGAA+TGG 0.456074 7.2:+88607097 MS.gene71534:CDS
TTCTCCTCCTTAGTCGGCAG+TGG 0.459284 7.2:+88606122 MS.gene71534:CDS
TAAGGTTGTTGGAGCAAAGA+AGG 0.462298 7.2:+88607132 MS.gene71534:CDS
TACGGGGCCACTGCCGACTA+AGG 0.463959 7.2:-88606129 None:intergenic
TTCAGCACAAAGACAGCAAT+GGG 0.470933 7.2:+88606092 MS.gene71534:CDS
TGATTGATTTCTCTGCTACA+TGG 0.471205 7.2:+88606356 MS.gene71534:CDS
TGATGATTCTGATGACTGTA+CGG 0.487264 7.2:-88606147 None:intergenic
GAATGGGAAAGAGGTTGATA+AGG 0.491854 7.2:+88607114 MS.gene71534:CDS
GATGATTCTGATGACTGTAC+GGG 0.500909 7.2:-88606146 None:intergenic
GATTTCTCTGCTACATGGTG+TGG 0.519111 7.2:+88606361 MS.gene71534:CDS
GGCACAAGAGTTTAAGGTGC+AGG 0.519916 7.2:+88607066 MS.gene71534:CDS
ATCAGTGAATTCATTAGCCA+TGG 0.533887 7.2:-88606416 None:intergenic
GTGTGGACCTTGTAAAATGA+TGG 0.541235 7.2:+88606378 MS.gene71534:CDS
CCTTCCACTCATCTGCTCGC+TGG 0.549073 7.2:+88606189 MS.gene71534:CDS
GTTGTTGAAGAATGGGAAAG+AGG 0.553595 7.2:+88607105 MS.gene71534:CDS
AGATGAGTGGAAGGTCTTGA+CGG 0.570930 7.2:-88606180 None:intergenic
GAATGTTGGAGTTGTTCTTG+TGG 0.588851 7.2:-88606072 None:intergenic
GGGGCCACTGCCGACTAAGG+AGG 0.594415 7.2:-88606126 None:intergenic
GTTGCCAGCGAGCAGATGAG+TGG 0.605035 7.2:-88606193 None:intergenic
TTCTTCAACAACACAAACGT+TGG 0.610426 7.2:-88607094 None:intergenic
ATTCAGCACAAAGACAGCAA+TGG 0.613497 7.2:+88606091 MS.gene71534:CDS
CCAGCGAGCAGATGAGTGGA+AGG 0.627549 7.2:-88606189 None:intergenic
GGAACCGATTCTTCAAGCCA+TGG 0.630372 7.2:+88606399 MS.gene71534:CDS
ATGATTCTGATGACTGTACG+GGG 0.715538 7.2:-88606145 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAAATAGAAAAATAAAA+TGG - chr7.2:88606291-88606310 None:intergenic 10.0%
!!! CATTTTATTTTTCTATTTTT+TGG + chr7.2:88606289-88606308 MS.gene71534:intron 10.0%
!!! TCATTTTTACAAATTATTAT+CGG + chr7.2:88606954-88606973 MS.gene71534:intron 10.0%
!!! TGATAATAGTAAATTTTAAA+AGG - chr7.2:88606787-88606806 None:intergenic 10.0%
!!! AATTATACAAAAAGCATTTA+AGG + chr7.2:88606742-88606761 MS.gene71534:intron 15.0%
!!! AGTAAATTTTAAAAGGAAAA+AGG - chr7.2:88606780-88606799 None:intergenic 15.0%
!!! ATAATGTTTTAATTTTTGTG+TGG + chr7.2:88607018-88607037 MS.gene71534:intron 15.0%
!!! ATTTTAAAAGGAAAAAGGTT+AGG - chr7.2:88606775-88606794 None:intergenic 20.0%
!!! TTAATTTTTGTGTGGTTTTT+TGG + chr7.2:88607026-88607045 MS.gene71534:intron 20.0%
! ATTGAGATCTAAAGAGATAT+GGG - chr7.2:88606260-88606279 None:intergenic 25.0%
! TATTGAGATCTAAAGAGATA+TGG - chr7.2:88606261-88606280 None:intergenic 25.0%
! TTCACAAACAGAAAATCAAA+AGG - chr7.2:88606331-88606350 None:intergenic 25.0%
! TTCTCAAAGTTTATTATAGC+AGG - chr7.2:88606700-88606719 None:intergenic 25.0%
!! AATCGATGTTGATGAATTAT+CGG + chr7.2:88606453-88606472 MS.gene71534:CDS 25.0%
!! AATTCTGTTATTTGTTGCAA+TGG + chr7.2:88606610-88606629 MS.gene71534:intron 25.0%
!! TGAATCAATTTTTGACCATA+CGG - chr7.2:88606828-88606847 None:intergenic 25.0%
!!! TTTTGATTTTCTGTTTGTGA+AGG + chr7.2:88606330-88606349 MS.gene71534:intron 25.0%
AACACAACAGTTCATCAAAT+TGG - chr7.2:88606493-88606512 None:intergenic 30.0%
ACACAACAGTTCATCAAATT+GGG - chr7.2:88606492-88606511 None:intergenic 30.0%
GAAGGATGAACTTAAAAACA+AGG + chr7.2:88607150-88607169 MS.gene71534:CDS 30.0%
TTAAAAGGAAAAAGGTTAGG+TGG - chr7.2:88606772-88606791 None:intergenic 30.0%
! TGGTCAAAAATTGATTCACT+CGG + chr7.2:88606830-88606849 MS.gene71534:intron 30.0%
! TTCTTTAGATGTTTTTCGTG+TGG + chr7.2:88606649-88606668 MS.gene71534:intron 30.0%
!! ATTTTTGTGTGGTTTTTTGG+TGG + chr7.2:88607029-88607048 MS.gene71534:intron 30.0%
!! TTTTTGTGTGGTTTTTTGGT+GGG + chr7.2:88607030-88607049 MS.gene71534:intron 30.0%
ATCAGTGAATTCATTAGCCA+TGG - chr7.2:88606419-88606438 None:intergenic 35.0%
CACAACAGTTCATCAAATTG+GGG - chr7.2:88606491-88606510 None:intergenic 35.0%
TGATGATTCTGATGACTGTA+CGG - chr7.2:88606150-88606169 None:intergenic 35.0%
TTCTTCAACAACACAAACGT+TGG - chr7.2:88607097-88607116 None:intergenic 35.0%
! ACGTTTGTGTTGTTGAAGAA+TGG + chr7.2:88607097-88607116 MS.gene71534:CDS 35.0%
! CGTTTGTGTTGTTGAAGAAT+GGG + chr7.2:88607098-88607117 MS.gene71534:CDS 35.0%
! CTGTTATTTGTTGCAATGGA+TGG + chr7.2:88606614-88606633 MS.gene71534:intron 35.0%
! TGATTGATTTCTCTGCTACA+TGG + chr7.2:88606356-88606375 MS.gene71534:CDS 35.0%
! TGATTTTCTGTTTGTGAAGG+TGG + chr7.2:88606333-88606352 MS.gene71534:intron 35.0%
!! AAAGAGGTTGATAAGGTTGT+TGG + chr7.2:88607121-88607140 MS.gene71534:CDS 35.0%
!! ATCGGTTCCATCATTTTACA+AGG - chr7.2:88606388-88606407 None:intergenic 35.0%
!! TCATTTTTCTCCTCCTTAGT+CGG + chr7.2:88606116-88606135 MS.gene71534:CDS 35.0%
!!! GGTTTTTTGGTGGGAATTAT+AGG + chr7.2:88607039-88607058 MS.gene71534:intron 35.0%
AAAATTTCGCACCCACACTT+CGG + chr7.2:88606527-88606546 MS.gene71534:intron 40.0%
ATGATTCTGATGACTGTACG+GGG - chr7.2:88606148-88606167 None:intergenic 40.0%
ATTCAGCACAAAGACAGCAA+TGG + chr7.2:88606091-88606110 MS.gene71534:CDS 40.0%
GAATGTTGGAGTTGTTCTTG+TGG - chr7.2:88606075-88606094 None:intergenic 40.0%
GATGATTCTGATGACTGTAC+GGG - chr7.2:88606149-88606168 None:intergenic 40.0%
GTGTGGACCTTGTAAAATGA+TGG + chr7.2:88606378-88606397 MS.gene71534:CDS 40.0%
GTTGTTGAAGAATGGGAAAG+AGG + chr7.2:88607105-88607124 MS.gene71534:CDS 40.0%
TAAGGTTGTTGGAGCAAAGA+AGG + chr7.2:88607132-88607151 MS.gene71534:CDS 40.0%
TTAGCCATGGCTTGAAGAAT+CGG - chr7.2:88606406-88606425 None:intergenic 40.0%
TTCAGCACAAAGACAGCAAT+GGG + chr7.2:88606092-88606111 MS.gene71534:CDS 40.0%
TTGGTGGGAATTATAGGATG+TGG + chr7.2:88607045-88607064 MS.gene71534:intron 40.0%
TTTGACCATACGGTCTAACA+TGG - chr7.2:88606818-88606837 None:intergenic 40.0%
! TGTGGTTGACTTTACTTGCT+TGG - chr7.2:88606057-88606076 None:intergenic 40.0%
!! GAATGGGAAAGAGGTTGATA+AGG + chr7.2:88607114-88607133 MS.gene71534:CDS 40.0%
AGATGAGTGGAAGGTCTTGA+CGG - chr7.2:88606183-88606202 None:intergenic 45.0%
CTTGTCCATGTTAGACCGTA+TGG + chr7.2:88606810-88606829 MS.gene71534:intron 45.0%
GATTTCTCTGCTACATGGTG+TGG + chr7.2:88606361-88606380 MS.gene71534:CDS 45.0%
GCTGTCTTTGTGCTGAATGT+TGG - chr7.2:88606089-88606108 None:intergenic 45.0%
GGATGTGGCACAAGAGTTTA+AGG + chr7.2:88607060-88607079 MS.gene71534:intron 45.0%
! GATATGGGTTTTACGAGACG+AGG - chr7.2:88606245-88606264 None:intergenic 45.0%
! GGTTCAAAAGCACAAAGCGT+AGG + chr7.2:88607171-88607190 MS.gene71534:CDS 45.0%
AAGCCTTCTCTCCGAAGTGT+GGG - chr7.2:88606541-88606560 None:intergenic 50.0%
TAAGCCTTCTCTCCGAAGTG+TGG - chr7.2:88606542-88606561 None:intergenic 50.0%
! GGAACCGATTCTTCAAGCCA+TGG + chr7.2:88606399-88606418 MS.gene71534:CDS 50.0%
! GGCACAAGAGTTTAAGGTGC+AGG + chr7.2:88607066-88607085 MS.gene71534:CDS 50.0%
TTCTCCTCCTTAGTCGGCAG+TGG + chr7.2:88606122-88606141 MS.gene71534:CDS 55.0%
CCAGCGAGCAGATGAGTGGA+AGG - chr7.2:88606192-88606211 None:intergenic 60.0%
CCTTCCACTCATCTGCTCGC+TGG + chr7.2:88606189-88606208 MS.gene71534:CDS 60.0%
GCACCCACACTTCGGAGAGA+AGG + chr7.2:88606535-88606554 MS.gene71534:intron 60.0%
GTTGCCAGCGAGCAGATGAG+TGG - chr7.2:88606196-88606215 None:intergenic 60.0%
! TACGGGGCCACTGCCGACTA+AGG - chr7.2:88606132-88606151 None:intergenic 65.0%
! GGGGCCACTGCCGACTAAGG+AGG - chr7.2:88606129-88606148 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 88606045 88607192 88606045 ID=MS.gene71534
chr7.2 mRNA 88606045 88607192 88606045 ID=MS.gene71534.t1;Parent=MS.gene71534
chr7.2 exon 88606045 88606250 88606045 ID=MS.gene71534.t1.exon1;Parent=MS.gene71534.t1
chr7.2 CDS 88606045 88606250 88606045 ID=cds.MS.gene71534.t1;Parent=MS.gene71534.t1
chr7.2 exon 88606352 88606474 88606352 ID=MS.gene71534.t1.exon2;Parent=MS.gene71534.t1
chr7.2 CDS 88606352 88606474 88606352 ID=cds.MS.gene71534.t1;Parent=MS.gene71534.t1
chr7.2 exon 88607061 88607192 88607061 ID=MS.gene71534.t1.exon3;Parent=MS.gene71534.t1
chr7.2 CDS 88607061 88607192 88607061 ID=cds.MS.gene71534.t1;Parent=MS.gene71534.t1
Gene Sequence

>MS.gene71534

GTGTAGTAACCAAGCAAGTAAAGTCAACCACAAGAACAACTCCAACATTCAGCACAAAGACAGCAATGGGATCATTTTTCTCCTCCTTAGTCGGCAGTGGCCCCGTACAGTCATCAGAATCATCAGAGAGTTCTTCCGTCAAGACCTTCCACTCATCTGCTCGCTGGCAACTTCACTTCAACGAACTCAAAGATTCTCCTCGTCTCGTAAAACCCATATCTCTTTAGATCTCAATAATTTTAACCATTTTATTTTTCTATTTTTTGGATCTGAAATTCTGAATCCTTTTGATTTTCTGTTTGTGAAGGTGGTGATTGATTTCTCTGCTACATGGTGTGGACCTTGTAAAATGATGGAACCGATTCTTCAAGCCATGGCTAATGAATTCACTGATGTTGAATTCATCAAAATCGATGTTGATGAATTATCGGTAATAATCTTATCCCCAATTTGATGAACTGTTGTGTTTTTAATTTCTGTAAAAAATTTCGCACCCACACTTCGGAGAGAAGGCTTATCACACTCGTGAGCATCTCATACAACACTTACACATGTGATCACATTCAATTCTGTTATTTGTTGCAATGGATGGAAACTAGTACATTTCTTTAGATGTTTTTCGTGTGGTTCTTAACTAAAAAATACATTTTTGCCTGCTATAATAAACTTTGAGAAAATCATGAGTAGTTTAATTCAGAATTATACAAAAAGCATTTAAGGAAATCCACCTAACCTTTTTCCTTTTAAAATTTACTATTATCATGTCTTGTCCATGTTAGACCGTATGGTCAAAAATTGATTCACTCGGAGTCGATGTGTCATTATTTGCATCAATGCTTTGTAGCATATTTATTACGTGTTTGTATTGTATCATAGTACAATACTAACACATGATTACATTCAATTATGTCATTTTTACAAATTATTATCGGTGTAGATGTGTTAATATGTGTTATTGCTTCTTAGCATATTTATAATGTTTTAATTTTTGTGTGGTTTTTTGGTGGGAATTATAGGATGTGGCACAAGAGTTTAAGGTGCAGGCTATGCCAACGTTTGTGTTGTTGAAGAATGGGAAAGAGGTTGATAAGGTTGTTGGAGCAAAGAAGGATGAACTTAAAAACAAGGTTCAAAAGCACAAAGCGTAG

Protein sequence

>MS.gene71534.t1

VUUPSKUSQPQEQLQHSAQRQQWDHFSPPUSAVAPYSHQNHQRVLPSRPSTHLLAGNFTSTNSKILLVSWULISLLHGVDLVKUWNRFFKPWLMNSLMLNSSKSMLMNYRMWHKSLRCRLCQRLCCURMGKRLIRLLEQRRMNLKTRFKSTKR